CN111437267B - Preparation method of rice bran polysaccharide-based composite nanoparticles - Google Patents
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Abstract
The invention discloses a preparation method of rice bran polysaccharide-based composite nanoparticles, and relates to the technical field of biological medicines. The preparation method of the rice bran polysaccharide-based composite nano-particles comprises the following steps: respectively dissolving rice bran polysaccharide, polylactic acid, N-dicyclohexylcarbodiimide and 4-dimethylaminopyridine in an organic solvent to correspondingly form a rice bran polysaccharide solution, a polylactic acid solution, an N, N-dicyclohexylcarbodiimide solution and a 4-dimethylaminopyridine solution; mixing and reacting the rice bran polysaccharide solution, the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution to form a reaction solution; and performing running water dialysis on the reaction solution, collecting trapped fluid, and drying to obtain the rice bran polysaccharide-based composite nano-particles. The invention aims to provide rice bran polysaccharide-based composite nano particles which can be infused into an organism in an intravenous injection mode.
Description
Technical Field
The invention relates to the technical field of biological medicines, and particularly relates to a preparation method of rice bran polysaccharide-based composite nanoparticles.
Background
The rice bran is a main byproduct in the rice processing industry, a small part of the rice bran is applied to the extraction of rice bran oil, rice bran protein and the like, a large amount of rice bran is directly applied to the feed processing industry every year, and the utilization rate and the added value of the rice bran are reduced. The rice bran polysaccharide is one of main components in rice bran, has various physiological activities, has the effects of improving immunity, resisting tumors and the like, has good market prospect, but has low utilization rate when being directly taken, and limits the application of the rice bran polysaccharide.
Disclosure of Invention
The invention mainly aims to provide a preparation method of rice bran polysaccharide-based composite nanoparticles, and aims to provide rice bran polysaccharide-based composite nanoparticles which can be infused into an organism in an intravenous injection mode.
In order to achieve the above object, the present invention provides a method for preparing rice bran polysaccharide-based composite nanoparticles, comprising the steps of:
respectively dissolving rice bran polysaccharide, polylactic acid, N-dicyclohexylcarbodiimide and 4-dimethylaminopyridine in an organic solvent to correspondingly form a rice bran polysaccharide solution, a polylactic acid solution, an N, N-dicyclohexylcarbodiimide solution and a 4-dimethylaminopyridine solution;
mixing and reacting the rice bran polysaccharide solution, the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution to form a reaction solution;
and performing running water dialysis on the reaction solution, collecting trapped fluid, and drying to obtain the rice bran polysaccharide-based composite nano-particles.
Optionally, the organic solvent comprises dimethyl sulfoxide.
Optionally, the step of taking rice bran polysaccharide, polylactic acid, N-dicyclohexylcarbodiimide and 4-dimethylaminopyridine to dissolve in an organic solvent respectively to form a rice bran polysaccharide solution, a polylactic acid solution, an N, N-dicyclohexylcarbodiimide solution and a 4-dimethylaminopyridine solution respectively comprises the steps of:
taking rice bran polysaccharide, adding an organic solvent, performing ultrasonic dissolution, centrifuging, taking supernate, performing ultrasonic treatment, and filtering by using an organic filter membrane to obtain a rice bran polysaccharide solution, wherein 5ml of the organic solvent is correspondingly added into every 90-110 mg of the rice bran polysaccharide;
adding the polylactic acid into the organic solvent, performing ultrasonic treatment, and filtering by using an organic filter membrane to obtain a polylactic acid solution, wherein 3ml of the organic solvent is correspondingly added into every 45-55 mg of the polylactic acid;
adding N, N-dicyclohexylcarbodiimide into the organic solvent, performing ultrasonic treatment, and filtering by using an organic filter membrane to obtain an N, N-dicyclohexylcarbodiimide solution, wherein 10ml of the organic solvent is correspondingly added into every 18-22 mg of the N, N-dicyclohexylcarbodiimide;
adding 4-dimethylaminopyridine into the organic solvent, performing ultrasonic treatment, and filtering by using an organic filter membrane to obtain a 4-dimethylaminopyridine solution, wherein 10ml of the organic solvent is added into every 8-12 mg of the 4-dimethylaminopyridine.
Optionally, the steps of taking the rice bran polysaccharide, adding an organic solvent, performing ultrasonic dissolution, centrifuging, taking supernate, performing ultrasonic treatment, and then filtering by using an organic filter membrane to obtain the rice bran polysaccharide solution comprise:
taking rice bran polysaccharide, adding an organic solvent, performing ultrasonic dissolution, centrifuging at the rotating speed of 500-1500 r/min for 4-6 min, taking supernate, performing ultrasonic treatment for 8-12 min, and filtering by adopting an organic filter membrane to obtain a rice bran polysaccharide solution.
Optionally, in the step of mixing and reacting the rice bran polysaccharide solution, the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution to form a reaction solution, the volume ratio of the rice bran polysaccharide solution, the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution is 5: 1.8-2.2: 0.5-0.9: 0.4-0.5.
Optionally, the step of mixing and reacting the rice bran polysaccharide solution, the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution to form a reaction solution comprises:
adding the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution into the rice bran polysaccharide solution, and reacting in a constant-temperature shaking table for 2-4 days to form a reaction solution, wherein the rotating speed of the constant-temperature shaking table is 180-220 r/min, and the temperature is 35-45 ℃.
Optionally, in the step of performing running water dialysis on the reaction solution, collecting trapped fluid, and drying to obtain the rice bran polysaccharide-based composite nanoparticles, the molecular weight cut-off of a dialysis bag used in the dialysis is not less than 14000.
Optionally, in the step of performing running water dialysis on the reaction solution, collecting trapped fluid, and drying to obtain the rice bran polysaccharide-based composite nanoparticles, the dialysis time is 2-4 days.
Optionally, in the step of performing running water dialysis on the reaction solution, collecting trapped fluid, and drying to obtain the rice bran polysaccharide-based composite nanoparticles, the particle size of the rice bran polysaccharide-based composite nanoparticles is 300nm to 190 nm.
According to the technical scheme provided by the invention, a rice bran polysaccharide solution, a polylactic acid solution, an N, N-dicyclohexylcarbodiimide solution and a 4-dimethylaminopyridine solution are mixed and reacted to construct rice bran polysaccharide-based composite nanoparticles with rice bran polysaccharide as a carrier, and the rice bran polysaccharide is wrapped by a polylactic acid nano material to form nanoscale particles.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other related drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a schematic flow chart of an embodiment of a method for preparing rice bran polysaccharide-based composite nanoparticles according to the present invention.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments.
It should be noted that those whose specific conditions are not specified in the examples were performed according to the conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially. In addition, the meaning of "and/or" appearing throughout includes three juxtapositions, exemplified by "A and/or B" including either A or B or both A and B. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The rice bran is a main byproduct in the rice processing industry, a small part of the rice bran is applied to the extraction of rice bran oil, rice bran protein and the like, a large amount of rice bran is directly applied to the feed processing industry every year, and the utilization rate and the added value of the rice bran are reduced. The rice bran polysaccharide is one of main components in rice bran, has various physiological activities, has the effects of improving immunity, resisting tumors and the like, has good market prospect, but has low utilization rate when being directly taken, and limits the application of the rice bran polysaccharide.
In view of the above, the invention provides a preparation method of rice bran polysaccharide-based composite nanoparticles, and the rice bran polysaccharide-based composite nanoparticles prepared by the method have small particle size and can be infused into an organism in an intravenous injection mode. Fig. 1 is a view illustrating an embodiment of a method for preparing rice bran polysaccharide-based composite nanoparticles according to the present invention.
Referring to fig. 1, the method for preparing rice bran polysaccharide-based composite nanoparticles includes the following steps:
step S10, rice bran polysaccharide, polylactic acid, N-dicyclohexylcarbodiimide and 4-dimethylaminopyridine are respectively dissolved in an organic solvent to correspondingly form a rice bran polysaccharide solution, a polylactic acid solution, an N, N-dicyclohexylcarbodiimide solution and a 4-dimethylaminopyridine solution.
The organic solvent may be any solvent capable of dissolving the rice bran polysaccharide, the polylactic acid, the N, N-dicyclohexylcarbodiimide, and the 4-dimethylaminopyridine, and may be chloroform, acetone, or the like. Dimethyl sulfoxide is preferred as the organic solvent in this embodiment.
In a specific implementation, step S10 may be implemented according to the following steps:
s11, taking rice bran polysaccharide, adding an organic solvent, performing ultrasonic dissolution, centrifuging, taking supernate, performing ultrasonic treatment, and filtering by adopting an organic filter membrane to obtain a rice bran polysaccharide solution, wherein 5ml of the organic solvent is correspondingly added into every 90-110 mg of the rice bran polysaccharide;
step S12, adding the polylactic acid into the organic solvent, performing ultrasonic treatment, and filtering with an organic filter membrane to obtain a polylactic acid solution, wherein 3ml of the organic solvent is added into every 45-55 mg of the polylactic acid;
s13, adding N, N-dicyclohexylcarbodiimide into the organic solvent, performing ultrasonic treatment, and filtering with an organic filter membrane to obtain an N, N-dicyclohexylcarbodiimide solution, wherein 10ml of the organic solvent is correspondingly added into every 18-22 mg of the N, N-dicyclohexylcarbodiimide;
and step S14, adding 4-dimethylaminopyridine into the organic solvent, performing ultrasonic treatment, and filtering with an organic filter membrane to obtain a 4-dimethylaminopyridine solution, wherein 10ml of the organic solvent is added to 8-12 mg of the 4-dimethylaminopyridine.
In step S11, the processing procedure of the rice bran polysaccharides directly affects the particle size and distribution stability of the finally constructed rice bran polysaccharide-based composite nanoparticles. Based on this, in this embodiment, after the organic solvent is added to the rice bran polysaccharide, the mixture is subjected to ultrasonic treatment until the amount of undissolved solids in the organic solvent is not reduced, and then the mixture is centrifuged at a rotation speed of 500 to 1500r/min for 4 to 6min, then the supernatant is collected, subjected to ultrasonic treatment for 8 to 12min, and finally filtered by an organic filter membrane, so as to obtain the rice bran polysaccharide solution.
Step S20, mixing and reacting the rice bran polysaccharide solution, the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution to form a reaction solution.
Wherein the volume ratio of the rice bran polysaccharide solution to the polylactic acid solution to the N, N-dicyclohexylcarbodiimide solution to the 4-dimethylaminopyridine solution is 5: 1.8-2.2: 0.5-0.9: 0.4-0.5. It should be noted that the concentrations of the four solutions are limited by the mixing ratio of the solutes and the organic solvent in the steps S11 to S14, for example, the rice bran polysaccharide solution is prepared by the step S11: adding 5ml of organic solvent into every 90-110 mg of rice bran polysaccharide, performing ultrasonic dissolution, centrifuging, taking supernate, performing ultrasonic treatment, and filtering by adopting an organic filter membrane to obtain a rice bran polysaccharide solution.
Specifically, step S20 includes:
step S21, adding the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution into the rice bran polysaccharide solution, and reacting in a constant temperature shaking table for 2-4 days to form a reaction solution, wherein the rotation speed of the constant temperature shaking table is 180-220 r/min, and the temperature is 35-45 ℃.
Wherein, one day is 24 hours, and 2-4 hours are 48-96 hours.
And step S30, performing running water dialysis on the reaction solution, collecting trapped fluid, and drying to obtain the rice bran polysaccharide-based composite nano-particles.
Wherein, the molecular weight cut-off of the adopted dialysis bag is not less than 14000, and the dialysis bag with the molecular weight cut-off of 14000 is preferred; the dialysis time is preferably 2 to 4 days. The grain diameter of the rice bran polysaccharide-based composite nano-particles is 300 nm-190 nm.
According to the technical scheme provided by the invention, a rice bran polysaccharide solution, a polylactic acid solution, an N, N-dicyclohexylcarbodiimide solution and a 4-dimethylaminopyridine solution are mixed and reacted, in a mixed system environment, the N, N-dicyclohexylcarbodiimide and the 4-dimethylaminopyridine promote polylactic acid to be connected to the surface of the rice bran polysaccharide, so that the rice bran polysaccharide-based composite nano-particles taking the rice bran polysaccharide as a carrier are constructed, and the rice bran polysaccharide is wrapped by a polylactic acid nano-material, so that rice bran polysaccharide molecules are folded and formed into nano-scale particles, and compared with the rice bran polysaccharide, the particle size is greatly reduced, so that the rice bran polysaccharide can be absorbed by a human body through intravenous injection, the bioavailability of the rice bran polysaccharide is improved, and the application approach of the rice bran polysaccharide is widened.
The technical solutions of the present invention are further described in detail below with reference to specific examples and drawings, it should be understood that the following examples are merely illustrative of the present invention and are not intended to limit the present invention.
Example 1
(1) Taking 100mg of rice bran polysaccharide, adding 5ml of dimethyl sulfoxide, performing ultrasonic treatment for 10min, centrifuging at the rotating speed of 1000r/min for 5min, taking supernate, performing ultrasonic treatment for 10min, and filtering with an organic filter membrane to obtain a rice bran polysaccharide solution;
(2) taking 50mg of polylactic acid, adding 3ml of dimethyl sulfoxide, shaking uniformly, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain a polylactic acid solution;
(3) taking 20mg of N, N-dicyclohexylcarbodiimide, adding 10ml of dimethyl sulfoxide, shaking up, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain an N, N-dicyclohexylcarbodiimide solution;
(4) taking 10mg of 4-dimethylaminopyridine, adding 10ml of dimethyl sulfoxide, shaking up, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain a 4-dimethylaminopyridine solution;
(5) 2ml of polylactic acid solution, 0.7ml of N, N-dicyclohexylcarbodiimide solution and 0.45ml of 4-dimethylaminopyridine solution were added to 5ml of rice bran polysaccharide solution, and the mixture was reacted in a constant temperature shaker at 40 ℃ for 3 days at 200r/min to form a reaction solution.
(6) Dialyzing the reaction solution by flowing water with a dialysis bag with a molecular weight of 14000 for 3 days, collecting trapped fluid, and performing freeze drying treatment to obtain the rice bran polysaccharide-based composite nano-particles.
Example 2
(1) Taking 90mg of rice bran polysaccharide, adding 5ml of dimethyl sulfoxide, performing ultrasonic treatment for 10min, centrifuging at the rotating speed of 1500r/min for 4min, taking supernatant, performing ultrasonic treatment for 8min, and filtering with an organic filter membrane to obtain a rice bran polysaccharide solution;
(2) taking 45mg of polylactic acid, adding 3ml of dimethyl sulfoxide, shaking uniformly, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain a polylactic acid solution;
(3) taking 18mg of N, N-dicyclohexylcarbodiimide, adding 10ml of dimethyl sulfoxide, shaking up, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain an N, N-dicyclohexylcarbodiimide solution;
(4) adding 8mg of 4-dimethylaminopyridine into 10ml of dimethyl sulfoxide, shaking up, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain a 4-dimethylaminopyridine solution;
(5) 1.8ml of polylactic acid solution, 0.5ml of N, N-dicyclohexylcarbodiimide solution and 0.4ml of 4-dimethylaminopyridine solution were added to 5ml of rice bran polysaccharide solution, and reacted in a constant temperature shaker at 35 ℃ for 4 days at 180r/min to form a reaction solution.
(6) Dialyzing the reaction solution with flowing water by using a dialysis bag with the molecular weight of 14000 for 2 days, collecting trapped fluid, and performing freeze drying treatment to obtain the rice bran polysaccharide-based composite nano-particles.
Example 3
(1) Taking 95mg of rice bran polysaccharide, adding 5ml of dimethyl sulfoxide, performing ultrasonic treatment for 10min, centrifuging at the rotating speed of 1300r/min for 4.5min, taking supernatant, performing ultrasonic treatment for 9min, and filtering with an organic filter membrane to obtain a rice bran polysaccharide solution;
(2) adding 48mg of polylactic acid into 3ml of dimethyl sulfoxide, shaking uniformly, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain a polylactic acid solution;
(3) taking 19mg of N, N-dicyclohexylcarbodiimide, adding 10ml of dimethyl sulfoxide, shaking up, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain an N, N-dicyclohexylcarbodiimide solution;
(4) adding 9mg of 4-dimethylaminopyridine into 10ml of dimethyl sulfoxide, shaking up, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain a 4-dimethylaminopyridine solution;
(5) 1.9ml of polylactic acid solution, 0.6ml of N, N-dicyclohexylcarbodiimide solution and 0.42ml of 4-dimethylaminopyridine solution were added to 5ml of rice bran polysaccharide solution, and reacted in a constant temperature shaker at 190r/min and 38 ℃ for 3.5 days to form a reaction solution.
(6) Dialyzing the reaction solution with flowing water by a dialysis bag with a molecular weight of 14000 for 2.5 days, collecting trapped fluid, and performing freeze drying treatment to obtain the rice bran polysaccharide-based composite nano-particles.
Example 4
(1) Taking 105mg of rice bran polysaccharide, adding 5ml of dimethyl sulfoxide, performing ultrasonic treatment for 10min, centrifuging at the rotating speed of 800r/min for 5.5min, taking supernatant, performing ultrasonic treatment for 11min, and filtering with an organic filter membrane to obtain a rice bran polysaccharide solution;
(2) taking 52mg of polylactic acid, adding 3ml of dimethyl sulfoxide, shaking uniformly, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain a polylactic acid solution;
(3) taking 20mg of N, N-dicyclohexylcarbodiimide, adding 10ml of dimethyl sulfoxide, shaking up, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain an N, N-dicyclohexylcarbodiimide solution;
(4) taking 11mg of 4-dimethylaminopyridine, adding 10ml of dimethyl sulfoxide, shaking up, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain a 4-dimethylaminopyridine solution;
(5) 2.1ml of polylactic acid solution, 0.8ml of N, N-dicyclohexylcarbodiimide solution and 0.47ml of 4-dimethylaminopyridine solution were added to 5ml of rice bran polysaccharide solution, and the mixture was reacted in a constant temperature shaker at 42 ℃ for 2.8 days at 210r/min to form a reaction solution.
(6) Dialyzing the reaction solution with flowing water by using a dialysis bag with a molecular weight of 14000 for 2.7 days, collecting trapped fluid, and performing freeze drying treatment to obtain the rice bran polysaccharide-based composite nano-particles.
Example 5
(1) Taking 110mg of rice bran polysaccharide, adding 5ml of dimethyl sulfoxide, performing ultrasonic treatment for 10min, centrifuging at the rotating speed of 500r/min for 6min, taking supernatant, performing ultrasonic treatment for 12min, and filtering with an organic filter membrane to obtain a rice bran polysaccharide solution;
(2) adding 55mg of polylactic acid into 3ml of dimethyl sulfoxide, shaking uniformly, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain a polylactic acid solution;
(3) adding 10ml of dimethyl sulfoxide into 22mg of N, N-dicyclohexylcarbodiimide, shaking up, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain N, N-dicyclohexylcarbodiimide solution;
(4) taking 12mg of 4-dimethylaminopyridine, adding 10ml of dimethyl sulfoxide, shaking up, performing ultrasonic treatment for 10min, and filtering by adopting an organic filter membrane to obtain a 4-dimethylaminopyridine solution;
(5) 2.2ml of polylactic acid solution, 0.9ml of N, N-dicyclohexylcarbodiimide solution and 0.5ml of 4-dimethylaminopyridine solution were added to 5ml of rice bran polysaccharide solution, and the mixture was reacted in a constant temperature shaker at 45 ℃ at 220r/min for 2 days to form a reaction solution.
(6) Dialyzing the reaction solution with flowing water by using a dialysis bag with the molecular weight of 14000 for 4 days, collecting trapped fluid, and performing freeze drying treatment to obtain the rice bran polysaccharide-based composite nano-particles.
Comparative example 1
Compared with the example 1, the steps are the same except that the step (1) is changed into the steps of taking 100mg of rice bran polysaccharide, adding 5ml of dimethyl sulfoxide, carrying out ultrasonic treatment for 10min, centrifuging for 2min at the rotating speed of 300r/min, taking supernate, carrying out ultrasonic treatment for 6min, and then filtering by adopting an organic filter membrane to obtain the rice bran polysaccharide solution.
Comparative example 2
Compared with the example 1, the steps are the same except that the step (1) is changed into the steps of taking 100mg of rice bran polysaccharide, adding 5ml of dimethyl sulfoxide, carrying out ultrasonic treatment for 10min, centrifuging for 7min at the rotating speed of 2000r/min, taking supernate, carrying out ultrasonic treatment for 14min, and then filtering by adopting an organic filter membrane to obtain the rice bran polysaccharide solution.
The examples, the respective proportions and the particle size of the rice bran polysaccharides were measured using a laser particle sizer and recorded as shown in table 1.
TABLE 1 comparison of particle size
| Particle size (nm) | Particle size (nm) | ||
| Example 1 | 278 | Example 5 | 257 |
| Example 2 | 270 | Comparative example 1 | 398 |
| Example 3 | 280 | Comparative example 2 | Instability of the film |
| Example 4 | 285 | Rice bran polysaccharides | 25000Da |
As can be seen from the above table, the rice bran polysaccharide-based composite nanoparticles prepared in each example can form a stable dispersion system, and the particle size reaches the nanometer level, which meets the requirements, while comparative example 2 cannot form a stable dispersion system.
The above is only a preferred embodiment of the present invention, and it is not intended to limit the scope of the invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall be included in the scope of the present invention.
Claims (7)
1. A preparation method of rice bran polysaccharide-based composite nanoparticles is characterized by comprising the following steps:
taking rice bran polysaccharide, adding an organic solvent, performing ultrasonic dissolution, centrifuging at the rotating speed of 500-1500 r/min for 4-6 min, taking supernate, performing ultrasonic treatment for 8-12 min, and filtering by adopting an organic filter membrane to obtain a rice bran polysaccharide solution;
adding the polylactic acid into the organic solvent, performing ultrasonic treatment, and filtering by adopting an organic filter membrane to obtain a polylactic acid solution;
adding N, N-dicyclohexylcarbodiimide into the organic solvent, performing ultrasonic treatment, and filtering by using an organic filter membrane to obtain an N, N-dicyclohexylcarbodiimide solution;
adding 4-dimethylaminopyridine into the organic solvent, performing ultrasonic treatment, and filtering by adopting an organic filter membrane to obtain a 4-dimethylaminopyridine solution;
mixing and reacting the rice bran polysaccharide solution, the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution to form a reaction solution;
and performing running water dialysis on the reaction solution, collecting trapped fluid, and drying to obtain rice bran polysaccharide-based composite nano-particles, wherein the particle size of the rice bran polysaccharide-based composite nano-particles is 257 nm-300 nm.
2. The method for preparing rice bran polysaccharide-based composite nanoparticles according to claim 1, wherein the organic solvent is selected from dimethyl sulfoxide.
3. The method for preparing rice bran polysaccharide-based composite nanoparticles according to claim 1 or 2, wherein 5ml of the organic solvent is added per 90-110 mg of the rice bran polysaccharide;
adding 3ml of the organic solvent into every 45-55 mg of the polylactic acid;
correspondingly adding 10ml of the organic solvent into every 18-22 mg of the N, N-dicyclohexylcarbodiimide;
and correspondingly adding 10ml of the organic solvent into 8-12 mg of the 4-dimethylaminopyridine.
4. The method for preparing rice bran polysaccharide-based composite nanoparticles according to claim 3, wherein in the step of mixing and reacting the rice bran polysaccharide solution, the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution to form a reaction solution, the volume ratio of the rice bran polysaccharide solution to the polylactic acid solution to the N, N-dicyclohexylcarbodiimide solution to the 4-dimethylaminopyridine solution is 5: 1.8-2.2: 0.5-0.9: 0.4-0.5.
5. The method of preparing rice bran polysaccharide-based composite nanoparticles according to claim 1, wherein the step of mixing and reacting the rice bran polysaccharide solution, the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution to form a reaction solution comprises:
adding the polylactic acid solution, the N, N-dicyclohexylcarbodiimide solution and the 4-dimethylaminopyridine solution into the rice bran polysaccharide solution, and reacting in a constant-temperature shaking table for 2-4 days to form a reaction solution, wherein the rotating speed of the constant-temperature shaking table is 180-220 r/min, and the temperature is 35-45 ℃.
6. The method according to claim 1, wherein the reaction solution is subjected to running water dialysis, and the retentate is collected and dried to obtain the rice bran polysaccharide-based composite nanoparticles, and wherein a dialysis bag having a cut-off molecular weight of not less than 14000 is used during the dialysis.
7. The method according to claim 1, wherein the step of subjecting the reaction solution to running water dialysis, collecting the retentate, and drying the retentate to obtain the rice bran polysaccharide-based composite nanoparticles comprises the step of performing dialysis for 2 to 4 days.
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