CN111436390B - Temperature regulation culture system and method for reducing mortality of TiLV to tilapia - Google Patents
Temperature regulation culture system and method for reducing mortality of TiLV to tilapia Download PDFInfo
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- 241000276707 Tilapia Species 0.000 title claims abstract description 46
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 230000001105 regulatory effect Effects 0.000 claims abstract description 19
- 238000009395 breeding Methods 0.000 claims abstract description 13
- 238000009360 aquaculture Methods 0.000 claims abstract description 5
- 244000144974 aquaculture Species 0.000 claims abstract description 5
- 238000004891 communication Methods 0.000 claims abstract description 4
- 238000012545 processing Methods 0.000 claims description 27
- 238000001514 detection method Methods 0.000 claims description 15
- 230000001276 controlling effect Effects 0.000 claims description 12
- 241000251468 Actinopterygii Species 0.000 claims description 11
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
- A01K63/065—Heating or cooling devices
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/20—Control of temperature characterised by the use of electric means with sensing elements having variation of electric or magnetic properties with change of temperature
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a temperature regulation culture system and method for reducing the mortality of TiLV to tilapia, relates to the technical field of aquaculture, and solves the problem of huge economic loss to tilapia culture in the world due to infection, high disease speed and high mortality of lake Luo virus (TiLV), and the key points of the technical scheme are as follows: the device comprises a biosensor, a temperature sensor, a temperature regulating device and a processor module, wherein the biosensor, the temperature sensor and the temperature regulating device are all in communication connection with the processor module; by detecting the Luo lake virus in real time, the temperature change treatment can be carried out on the water body of the culture pond in time, and the favorable conditions of virus replication, breeding and breeding are changed, so that the virus is purified, and the fatality rate of tilapia infected with the Luo lake virus is reduced.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to a temperature regulation culture system and method for reducing the mortality of TiLV to tilapia.
Background
Tilapia mossambica is one of economic fish species of important freshwater aquaculture in the world and is an important source of animal protein in developing countries. Tilapia is originally Africa and belongs to tropical fishes, and the tilapia comprises more than 100 subspecies. Tilapia has the characteristics of fast generation, high yield, food impurities, few diseases, strong fecundity and the like. At present, tilapia is intensively cultured in tropical and subtropical areas such as Guangdong, Guangxi, Hainan and the like in China. However, in recent years, Thai, Israel, Ecuador and Egypt have successively outbreak of massive death of tilapia related to virus diseases, which causes great economic loss to the tilapia breeding industry. Through identification, the pathogens causing the massive death of Tilapia are all lake Luo virus (Tilapia lake virus, TiLV), the Tilapia lake virus is a negative strand RNA virus, and the genome consists of 10 independent gene segments. At present, the functions of viral proteins encoded by 10 fragments of the TiLV virus are not clear.
TiLV has been shown to be an advantageous condition for virus replication, propagation and propagation at 24-30 ℃ (Fathi et al, 2017). The massive death events of the tilapia cultivated in Egypt show that massive death occurs in the middle and large-size cultivation areas of the tilapia nilotica in the summer of 2012-2016 when the temperature of local water in Egypt is 25-30 ℃, the tilapia nilotica is called as a 'summer fish death event', and the tilapia nilotica is identified to be infected with TiLV. Ferguson and the like investigate and research mass death of the tilapia mossambica culture area caused by TiLV infection, and samples of the diseased fish of the Chitrada strain are collected for pathological section in 5 months (air temperature 27 ℃) in 2011, 7 months (air temperature 25 ℃), 11 months (air temperature 25 ℃) and 5 months (air temperature 27 ℃) in 2012, and the results show that the liver and the gastrointestinal tract are main diseased organs. Therefore, the culture temperature (especially in summer) is an important factor for high lethality rate of tilapia infected with Luo lake virus.
In 2017, in 5 months, the virus is detected from the domestic cultivated tilapia for the first time. However, at present, few studies are made on how to prevent and treat the Luo lake virus disease through temperature control in the tilapia culture process. And because the Rou lake virus is infected, the disease is fast to occur and the death rate is high, great loss is caused to the tilapia culture. Therefore, how to research and design a temperature regulation culture system and a method for reducing the mortality of TiLV to tilapia is a problem which is urgently needed to be solved at present.
Disclosure of Invention
The invention aims to provide a temperature-controlled culture system for reducing the death rate of TiLV to tilapia, which can timely carry out temperature-changing treatment on a culture pond water body by detecting the pathogenic bacteria of lake Roots virus in real time, change the favorable conditions of virus replication, breeding and breeding, passivate the virus and further reduce the death rate after infection of lake Roots virus.
The technical purpose of the invention is realized by the following technical scheme: a temperature regulation and control culture system for reducing the mortality rate of TiLV to tilapia comprises a biosensor, a temperature sensor, a temperature regulation device and a processor module, wherein the biosensor, the temperature sensor and the temperature regulation device are all in communication connection with the processor module; wherein the content of the first and second substances,
the biosensor is used for detecting the Luo lake virus in the water body of the tilapia culture pond and transmitting a detection signal to the processor module;
the temperature sensor is used for collecting a real-time temperature value of the water body of the tilapia mossambica culture pond and transmitting the real-time temperature value to the processor module;
the processor module is used for processing the detection signal and controlling the temperature sensor to start according to the processing result; calculating the number of days for temperature regulation and control and the temperature regulation degree of each day according to the initial real-time temperature value and the built-in standard temperature value, and generating a corresponding temperature regulation and control command;
and the temperature regulating device is used for heating the water body of the tilapia culture pond to a standard temperature value according to the temperature regulating and controlling command.
Preferably, the processor module comprises a storage unit, a processing unit, a first calculating unit, a second calculating unit and a selecting unit; wherein the content of the first and second substances,
the storage unit stores standard temperature values of the water body of the tilapia culture pond;
the processing unit is used for judging whether the fish body is infected with the Luo lake virus after signal processing is carried out on the detection signal, and generating a starting command for controlling the starting of the temperature sensor according to the judgment result; generating a temperature regulation and control command according to the operation results of the first calculating unit, the second calculating unit and the selecting unit;
the first calculating unit is used for calculating the number of days for temperature regulation and control according to the initial real-time temperature value and the standard temperature value; the calculation formula of the temperature regulation days is as follows: n ≈ N ═ T (T)0-T1) N is an integer obtained by rounding off the N value; wherein n is the number of days for temperature regulation and control, T0Is a standard temperature value, T1Is an initial real-time temperature value, T0-T1The total temperature regulation value is obtained;
the second calculating unit is used for calculating the temperature regulation degree corresponding to each day according to the total temperature regulation value and the temperature regulation days, and the calculation formula is as follows:
2a1n-(n-1)nd＝2(T0-T1),an＝a1-(n-1)d，a1...anare all positive numbers, d is a positive integer, a1Not more than 5; it is composed ofIn (a)1The degree of temperature regulation for the first day, anThe temperature regulation degree of the nth day;
and the selection unit is used for screening the data set obtained by the second calculation unit according to the standard with the minimum d value and sending the screened data set to the processing unit.
Preferably, the biological sensor and the temperature sensor are both arranged in the depth of one third to one fourth of the water in the culture pond.
Preferably, the temperature regulating device is one or more of a heating submersible pump, an electric heating thermostat and a heat exchanger.
The invention also aims to provide a temperature-controlled breeding method for reducing the mortality of TiLV to tilapia, which comprises the following steps:
s1: detecting the Luo lake virus in the water body of the tilapia culture pond by using a biosensor, and transmitting a detection signal to a processor module;
s2: processing the detection signal, and controlling the temperature sensor to start according to the processing result;
s3: collecting a real-time temperature value of a water body of a tilapia culture pond by using a temperature sensor, and transmitting the real-time temperature value to a processor module;
s4: calculating the number of days for temperature regulation and control and the temperature regulation degree of each day according to the initial real-time temperature value and a standard temperature value built in the processor module, and generating a corresponding temperature regulation and control command;
s5: and raising the temperature of the water body of the tilapia culture pond to a standard temperature value according to the temperature regulation and control command.
Preferably, the method for calculating the temperature regulation number of days and the temperature regulation degree per day specifically comprises the following steps:
s41: calculating the number of days for temperature regulation and control according to the initial real-time temperature value and the standard temperature value; the calculation formula of the temperature regulation days is as follows: n ≈ N ═ T (T)0-T1) N is an integer obtained by rounding off the N value; wherein n is the number of days for temperature regulation and control, T0Is a standard temperature value, T1Is an initial real-time temperature value, T0-T1Is a total ofA temperature regulation value;
s42: and calculating the temperature regulation degree corresponding to each day according to the total temperature regulation value and the temperature regulation days, wherein the calculation formula is as follows:
2a1n-(n-1)nd＝2(T0-T1),an＝a1-(n-1)d，a1...anare all positive numbers, d is a positive integer, a1Not more than 5; wherein, a1The degree of temperature regulation for the first day, anThe temperature regulation degree of the nth day;
s43: and screening the data set obtained by the second computing unit according to the standard with the minimum d value, and sending the screened data set to the processing unit.
Preferably, the standard temperature value T0The temperature was 35 ℃.
Preferably, the temperature of the culture pond is regulated and controlled to be increased in at least two stages within one day, and the interval time between two adjacent stages is at least 4 h.
Compared with the prior art, the invention has the following beneficial effects: by detecting the Rou lake virus pathogens in real time, the temperature of the water body of the culture pond can be raised in time, the favorable conditions of virus replication, breeding and breeding are changed, and the viruses are purified, so that the fatality rate after the Rou lake virus infection is reduced; by the regulation and control mode of regulating and controlling the temperature value to be decreased, emergency measures are quickly and effectively taken for the prevention and the treatment of the Luo lake virus, other diseases caused by sudden change of the temperature of the living environment of the fish body are prevented, the stress resistance of the fish body can be improved by the relatively stable living environment, and favorable conditions are provided for immune mechanism response.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed for the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic diagram showing the operation of embodiment 1 of the present invention;
fig. 2 is a flowchart in embodiment 2 of the present invention.
In the figure: 1. a biosensor; 2. a temperature sensor; 3. a processor module; 31. a storage unit; 32. a processing unit; 33. a first calculation unit; 34. a second calculation unit; 35. a selection unit; 4. a temperature regulating device.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments.
Example 1: a temperature regulation and control culture system for reducing the mortality rate of TiLV to tilapia is shown in figure 1 and comprises a biosensor 1, a temperature sensor 2, a temperature regulation device 4 and a processor module 3, wherein the biosensor 1, the temperature sensor 2 and the temperature regulation device 4 are all in communication connection with the processor module 3. The biosensor 1 is used for detecting the Luo lake virus in the water body of the tilapia culture pond and transmitting a detection signal to the processor module 3; the biosensor 1 comprises at least one biological substance that exerts a sensing effect on lake ro virus, such as: antibodies, enzymes. In the embodiment, the biological substance is colloidal gold antibody prepared from Luo lake virus particles, and once the water in the culture pond has virus, the biosensor 1 can capture and recognize the virus and then transmit signals. The temperature sensor 2 is used for collecting the real-time temperature value of the water body of the tilapia culture pond and transmitting the real-time temperature value to the processor module 3. The processor module 3 is used for processing the detection signal and controlling the temperature sensor 2 to start according to the processing result; and calculating the number of days for temperature regulation and control and the temperature regulation degree of each day according to the initial real-time temperature value and the built-in standard temperature value, and generating a corresponding temperature regulation and control command. The temperature adjusting device 4 is used for raising the temperature of the water body of the tilapia mossambica culture pond to a standard temperature value according to the temperature adjusting and controlling command.
The processor module 3 comprises a storage unit 31, a processing unit 32, a first calculation unit 33, a second calculation unit 34, a selection unit 35. Wherein:
the storage unit 31 stores standard temperature values of the water body of the tilapia culture pond.
The processing unit 32 is used for judging whether the fish body is infected with the lake ro virus after signal processing is carried out on the detection signal, and generating a starting command for controlling the temperature sensor 2 to start according to the judgment result; and generating a temperature regulation and control command according to the operation results of the first calculating unit 33, the second calculating unit 34 and the selecting unit 35.
The first calculating unit 33 is configured to calculate the number of days for temperature regulation according to the initial real-time temperature value and the standard temperature value; the formula for calculating the number of days for temperature regulation is as follows: n ≈ N ═ T (T)0-T1) N is an integer obtained by rounding off the N value; wherein n is the number of days for temperature regulation and control, T0Is a standard temperature value, T1Is an initial real-time temperature value, T0-T1Is the total temperature regulation value.
The second calculating unit 34 is configured to calculate the temperature control degree corresponding to each day according to the total temperature control value and the temperature control days, and the calculation formula is as follows:
2a1n-(n-1)nd＝2(T0-T1),an＝a1-(n-1)d，a1...anare all positive numbers, d is a positive integer, a1Not more than 5; wherein, a1The degree of temperature regulation for the first day, anThe temperature control degree on the nth day.
The selection unit 35 is configured to screen the data set obtained by the second calculation unit 34 by using the criterion that the value d is the smallest, and send the screened data set to the processing unit 32.
In this embodiment, the biosensor 1 and the temperature sensor 2 are both arranged in the water depth of one third to one fourth of the culture pond.
In the present embodiment, the temperature adjusting device 4 is one or more of a heating submersible pump, an electric heating thermostat, and a heat exchanger.
Example 2: a temperature-controlled breeding method for reducing the mortality of TiLV to tilapia as shown in figures 1 and 2 comprises the following steps:
s1: the biosensor 1 is used for detecting the Luo lake virus in the water body of the tilapia culture pond, and a detection signal is transmitted to the processor module 3.
S2: the detection signal is processed, and the temperature sensor 2 is controlled to start according to the processing result.
S3: and the temperature sensor 2 is used for collecting the real-time temperature value of the water body of the tilapia culture pond and transmitting the real-time temperature value to the processor module 3.
S4: and calculating the number of days for temperature regulation and control and the temperature regulation degree of each day according to the initial real-time temperature value and the standard temperature value built in the processor module 3, and generating a corresponding temperature regulation and control command.
S5: and raising the temperature of the water body of the tilapia culture pond to a standard temperature value according to the temperature regulation and control command.
The method for calculating the temperature regulation days and the temperature regulation degrees each day specifically comprises the following steps:
s41: calculating the number of days for temperature regulation and control according to the initial real-time temperature value and the standard temperature value; the formula for calculating the number of days for temperature regulation is as follows: n ≈ N ═ T (T)0-T1) N is an integer obtained by rounding off the N value; wherein n is the number of days for temperature regulation and control, T0Is a standard temperature value, T1Is an initial real-time temperature value, T0-T1Is the total temperature regulation value.
S42: and calculating the temperature regulation degree corresponding to each day according to the total temperature regulation value and the temperature regulation days, wherein the calculation formula is as follows:
2a1n-(n-1)nd＝2(T0-T1),an＝a1-(n-1)d，a1...anare all positive numbers, d is a positive integer, a1Not more than 5; wherein, a1The degree of temperature regulation for the first day, anThe temperature control degree on the nth day.
S43: the data set obtained by the second calculation unit 34 is filtered by the criterion of the smallest value of d, and the filtered data set is sent to the processing unit 32.
Standard temperature value T0The temperature was 35 ℃. The virus is rapidly propagated at 25-30 ℃, so after the virus is detected in the water body of the culture pond, the temperature of the water body needs to be raised to 35 ℃ to passivate the virus, and the replication of the virus is inhibited.
The temperature of the culture pond is regulated and controlled to be increased in at least two stages within one day, and the interval time between two adjacent stages is at least 4 h.
The working principle is as follows: by detecting the Rou lake virus pathogens in real time, the temperature change treatment can be timely carried out on the water body of the culture pond, the favorable conditions of virus replication, breeding and breeding are changed, and the viruses are purified, so that the fatality rate after the Rou lake virus infection is reduced; by the regulation and control mode of regulating and controlling the temperature value to be decreased, emergency measures are quickly and effectively taken for the prevention and the treatment of the Luo lake virus, other diseases caused by sudden change of the temperature of the living environment of the fish body are prevented, the stress resistance of the fish body can be improved by the relatively stable living environment, and favorable conditions are provided for immune mechanism response.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (6)
1. A temperature regulation culture system for reducing the mortality of TiLV to tilapia is characterized in that: the device comprises a biosensor (1), a temperature sensor (2), a temperature regulating device (4) and a processor module (3), wherein the biosensor (1), the temperature sensor (2) and the temperature regulating device (4) are in communication connection with the processor module (3); wherein the content of the first and second substances,
the biosensor (1) is used for detecting the Luo lake virus in the water body of the tilapia culture pond and transmitting a detection signal to the processor module (3);
the temperature sensor (2) is used for collecting a real-time temperature value of the water body of the tilapia mossambica culture pond and transmitting the real-time temperature value to the processor module (3);
the processor module (3) is used for processing the detection signal and controlling the temperature sensor (2) to start according to the processing result; calculating the number of days for temperature regulation and control and the temperature regulation degree of each day according to the initial real-time temperature value and the built-in standard temperature value, and generating a corresponding temperature regulation and control command;
the temperature regulating device (4) is used for heating the water body of the tilapia mossambica culture pond to a standard temperature value according to the temperature regulating command;
the processor module (3) comprises a storage unit (31), a processing unit (32), a first calculating unit (33), a second calculating unit (34) and a selecting unit (35); wherein the content of the first and second substances,
the storage unit (31) stores standard temperature values of the water body of the tilapia culture pond;
the processing unit (32) is used for judging whether the fish body is infected with the lake ro virus after signal processing is carried out on the detection signal, and generating a starting command for controlling the temperature sensor (2) to start according to the judgment result; and generating a temperature regulation and control command according to the operation results of the first calculating unit (33), the second calculating unit (34) and the selecting unit (35);
the first calculating unit (33) is used for calculating the number of days for temperature regulation and control according to the initial real-time temperature value and the standard temperature value; the calculation formula of the temperature regulation days is as follows: n ≈ N ═ T (T)0-T1) N is an integer obtained by rounding off the N value; wherein n is the number of days for temperature regulation and control, T0Is a standard temperature value, T1Is an initial real-time temperature value, T0-T1The total temperature regulation value is obtained;
the second calculating unit (34) is used for calculating the temperature regulation degree corresponding to each day according to the total temperature regulation value and the temperature regulation days, and the calculation formula is as follows:
2a1n-(n-1)nd＝2(T0-T1),an＝a1-(n-1)d，a1...anare all positive numbers, d is a positive integer, a1Not more than 5; wherein, a1The degree of temperature regulation for the first day, anThe temperature regulation degree of the nth day;
the selection unit (35) is used for screening the data set obtained by the second calculation unit (34) by the standard with the minimum d value, and sending the screened data set to the processing unit (32).
2. The temperature-regulated aquaculture system of claim 1 that reduces mortality of TiLV to tilapia, characterized by: the biological sensor (1) and the temperature sensor (2) are both arranged in the depth of one third to one fourth of the water in the culture pond.
3. The temperature-regulated aquaculture system of claim 1 that reduces mortality of TiLV to tilapia, characterized by: the temperature adjusting device (4) is one or more of a heating submersible pump, an electric heating thermostat and a heat exchanger.
4. A temperature regulation culture method for reducing the mortality of TiLV to tilapia is characterized in that: the method comprises the following steps:
s1: the method comprises the following steps of detecting the Rou lake virus in the water body of the tilapia culture pond by using a biosensor (1), and transmitting a detection signal to a processor module (3);
s2: processing the detection signal, and controlling the temperature sensor (2) to start according to the processing result;
s3: collecting a real-time temperature value of a water body of a tilapia mossambica culture pond by using a temperature sensor (2), and transmitting the real-time temperature value to a processor module (3);
s4: calculating the number of days for temperature regulation and control and the temperature regulation degree of each day according to the initial real-time temperature value and a standard temperature value built in the processor module (3), and generating a corresponding temperature regulation and control command;
s5: heating the water body of the tilapia culture pond to a standard temperature value according to the temperature regulation and control command;
the method for calculating the temperature regulation days and the temperature regulation degrees per day specifically comprises the following steps:
s41: calculating the number of days for temperature regulation and control according to the initial real-time temperature value and the standard temperature value; the calculation formula of the temperature regulation days is as follows: n ≈ N ═ T (T)0-T1) N is an integer obtained by rounding off the N value; wherein n is the number of days for temperature regulation and control, T0Is a standard temperature value, T1Is an initial real-time temperature value, T0-T1The total temperature regulation value is obtained;
s42: and calculating the temperature regulation degree corresponding to each day according to the total temperature regulation value and the temperature regulation days, wherein the calculation formula is as follows:
2a1n-(n-1)nd＝2(T0-T1),an＝a1-(n-1)d，a1...anare all positive numbers, d is a positive integer, a1Not more than 5; wherein, a1The degree of temperature regulation for the first day, anThe temperature regulation degree of the nth day;
s43: the data set obtained by the second computing unit (34) is screened according to the standard with the minimum d value, and the screened data set is sent to the processing unit (32).
5. The temperature-regulated breeding method for reducing the mortality of TiLV to tilapia according to claim 4, which is characterized in that: the standard temperature value T0The temperature was 35 ℃.
6. The temperature-regulated breeding method for reducing the mortality of TiLV to tilapia according to claim 4, which is characterized in that: the temperature of the culture pond is regulated and controlled to be increased in at least two stages within one day, and the interval time between two adjacent stages is at least 4 h.
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