CN111422994B - Application of bacillus pumilus and treatment method of nuclide wastewater or heavy metal wastewater - Google Patents
Application of bacillus pumilus and treatment method of nuclide wastewater or heavy metal wastewater Download PDFInfo
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- CN111422994B CN111422994B CN202010208520.7A CN202010208520A CN111422994B CN 111422994 B CN111422994 B CN 111422994B CN 202010208520 A CN202010208520 A CN 202010208520A CN 111422994 B CN111422994 B CN 111422994B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- G—PHYSICS
- G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
- G21F—PROTECTION AGAINST X-RADIATION, GAMMA RADIATION, CORPUSCULAR RADIATION OR PARTICLE BOMBARDMENT; TREATING RADIOACTIVELY CONTAMINATED MATERIAL; DECONTAMINATION ARRANGEMENTS THEREFOR
- G21F9/00—Treating radioactively contaminated material; Decontamination arrangements therefor
- G21F9/04—Treating liquids
- G21F9/06—Processing
- G21F9/12—Processing by absorption; by adsorption; by ion-exchange
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- G—PHYSICS
- G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
- G21F—PROTECTION AGAINST X-RADIATION, GAMMA RADIATION, CORPUSCULAR RADIATION OR PARTICLE BOMBARDMENT; TREATING RADIOACTIVELY CONTAMINATED MATERIAL; DECONTAMINATION ARRANGEMENTS THEREFOR
- G21F9/00—Treating radioactively contaminated material; Decontamination arrangements therefor
- G21F9/04—Treating liquids
- G21F9/06—Processing
- G21F9/18—Processing by biological processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/006—Radioactive compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
Abstract
The invention discloses an application of bacillus pumilus in nuclide adsorption or heavy metal ion adsorption, wherein the bacillus pumilus is bacillus pumilus S1419, and the strain preservation number is as follows: CGMCC No. 17816. The invention also discloses a treatment method of the nuclide wastewater or heavy metal wastewater, which is to adsorb nuclide or heavy metal ions in the wastewater through the bacillus pumilus S1419. The method can realize in-situ adsorption and enrichment of radioactive elements in the nuclide wastewater, has the advantages of simple operation, low cost, low energy consumption and no secondary pollutant, can effectively realize the reduction of radioactive wastes, has good adsorption effect on the wastewater containing low-concentration cobalt ions, can quickly perform adsorption reaction, can reach adsorption balance in a short time, and has high adsorption efficiency.
Description
Technical Field
The invention relates to the field of microorganism application, in particular to application of bacillus pumilus S1419 in nuclide adsorption or heavy metal ion adsorption and a method for treating nuclide wastewater or heavy metal wastewater.
Background
The two major problems of environmental pollution and energy shortage are becoming more serious, clean Nuclear energy becomes the second most low-carbon power source in the World, and the data published by World Nuclear Association in 2019 in 2 months indicates that: about 450 nuclear reactors have been built globally, 11% of support is provided for world electric power, and the nuclear power supply potential of China is third in the world and is one of the few countries with complete nuclear power industrial systems all over the world. Not only nuclear power, nuclide has promoted the development of scientific research fields such as aerospace nuclear battery, scientific isotope tracing and the like, and agricultural breeding, radiation sterilization and disease treatment in daily life have also been rapidly popularized. In the process of nuclide manufacturing, transportation and application, a small amount of radioactive substances enter a cooling water system due to the breakage of fuel rods and the like, some corrosion products are also activated into radionuclides after entering a reactor core along with cooling water, and meanwhile, a small amount of nuclides are obtained by elution when a shielding transportation container and nuclide related instruments in the nuclide packaging process are cleaned after being used, so that the wastewater containing the nuclides must be effectively treated for the ecological environment and human health around the nuclide application place, and the problem must become a big hot spot problem in the aspect of future environmental treatment.
The radioactive substances difficult to treat in the nuclide wastewater are mostly low-concentration ionic radioactive metals, and the common radioactive substances include five kinds of uranium, iodine, cesium, cobalt and strontium, wherein the five kinds of uranium, iodine, cesium, cobalt and strontium are included58Co、60Co is most widely applied in the industries of industry, agriculture and medical treatment, has a half-life of several to dozens of years, can release gamma rays with great harm, and is inconvenient to store and difficult to process. CN101497483A and CN103617818A use a plurality of physicochemical treatment processes to fix nuclides and heavy metal ions, but not only use a large amount of pyrite powder or a plurality of mixed acids, but also have complicated treatment processes and more required devices, thereby greatly increasing the treatment risk of nuclide wastewater. The modular reactor designed in the CN105719717A nuclear decontamination process uses a multi-membrane process, and the membranes replaced subsequently increase the volume of secondary pollutants, so that the danger of the equipment maintenance process is great. The method has the advantages that the radionuclide wastewater is treated by microorganisms, the in-situ adsorption, enrichment and even oxidation reduction of radioactive elements in the radionuclide wastewater can be realized, the reduction of radioactive wastes can be effectively realized, the bacillus pumilus has stronger ionizing radiation capability and can be used as an indicator for detecting the ionizing radiation sterilization effect in many countries, and if the application of the bacillus pumilus in the radionuclide wastewater treatment can be expanded, the bacillus pumilus has extremely high application valueMeaning.
Disclosure of Invention
The invention aims to provide application of bacillus pumilus and a method for treating nuclide wastewater or heavy metal wastewater, which are used for simply and quickly adsorbing nuclide in wastewater and reducing the concentration of radioactive elements in the wastewater.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention discloses application of bacillus pumilus in nuclide adsorption or heavy metal ion adsorption. The bacillus pumilus is bacillus pumilus S1419, and the strain preservation number is as follows: CGMCC No. 17816.
Further, the nuclide is60Co or58Co, the heavy metal ion is Co2+Ions.
The invention also discloses a method for treating the nuclide wastewater or the heavy metal wastewater, which adopts the bacillus pumilus S1419 to treat the nuclide wastewater, and adsorbs nuclide or heavy metal ions in the wastewater through the bacillus pumilus S1419. The bacillus pumilus strain S1419 has a accession number: CGMCC No. 17816.
The nuclide wastewater or heavy metal wastewater contains Co2+Ions.
Further, the nuclide wastewater or heavy metal wastewater also contains Cr3+、Mn2+、Fe3+One or more of ions.
The nuclide wastewater or heavy metal wastewater treatment method comprises the following steps:
s1, taking a strain of the Bacillus pumilus S1419, inoculating the strain to an LB solid culture medium by using an inoculating loop, and performing static culture in a constant temperature incubator at 25-35 ℃ for 24-25 h.
S2, taking a single colony growing from the solid culture medium by using an inoculating loop, inoculating the single colony to an LB liquid culture medium, and performing shake culture at the temperature of 25-35 ℃ and the rotating speed of 140-160 r/min for 24-25 h to obtain a bacterial liquid.
S3, centrifuging or filtering or naturally settling the obtained bacterial liquid, collecting the bacillus pumilus S1419 thallus precipitated at the bottom of a centrifugal tube, suspending the obtained thallus in an NaCl solution with the same volume and the mass fraction of 0.9-1%, washing off substances adhered to the surfaces of the thallus, and repeatedly washing for a plurality of times.
S4, resuspending the cleaned thallus into nuclide wastewater or heavy metal wastewater, and carrying out adsorption reaction in a temperature control shaking table at the temperature of 25-35 ℃ and the rotating speed of 150-200 r/min.
And S5, after the adsorption reaction, removing the bacillus pumilus in the treated nuclide wastewater or heavy metal wastewater through centrifugation, filtration or natural sedimentation.
Further, the bacteria in step S4 are resuspended in the nuclide wastewater or heavy metal wastewater, and the OD of Bacillus pumilus S1419 in the nuclide wastewater or heavy metal wastewater is adjusted to 1.2-1.3 by a spectrophotometer.
Preferably, said Co2+The initial concentration of the ions is less than or equal to 5 mg/L.
Preferably, the bacterial liquid obtained in the step S3 is centrifuged at 5000-6000 r/min and 20 ℃ for 5-10 min, and then the bacillus pumilus S1419 bacteria precipitated at the bottom of the centrifugal tube are collected.
Further, before the bacteria are put into the nuclide wastewater or heavy metal wastewater in the step S4, adding a NaOH solution into the nuclide wastewater or heavy metal wastewater to adjust the pH value to 5.0-8.1; and step S5, removing the bacillus pumilus in the treated nuclide wastewater or heavy metal wastewater after the adsorption reaction time is 3-5 min.
After the technical scheme is adopted, the invention has the following effects:
1. the invention utilizes the bacillus pumilus S1419 to adsorb and treat the nuclide wastewater or the heavy metal, can realize the in-situ adsorption and enrichment of the radioactive elements, has the advantages of simple operation, low cost and low energy consumption, and can effectively realize the reduction of the radioactive wastes.
2. The method of the invention can treat the nuclide wastewater or the heavy metal wastewater without additional equipment, and after the adsorption reaction, the thalli can be separated by standing, settling, centrifuging, filtering and other methods, thereby having no secondary pollution.
3. The invention selects and uses the preservation number as follows: the Bacillus pumilus S1419 with CGMCC No.17816 still has good adsorption effect on waste water containing low-concentration cobalt ions (the initial concentration is less than or equal to 5.0 mg/L), can quickly perform adsorption reaction, can reach adsorption balance within 3-5 min of extremely short adsorption time, and has high adsorption efficiency.
Drawings
FIG. 1 is a scanning electron micrograph of Bacillus pumilus S1419 strain of the present invention.
FIG. 2 shows the residual concentration of cobalt ions in the reaction solution with time during the adsorption reaction.
FIG. 3 shows the amount of cobalt ions adsorbed by cells with time during the adsorption reaction.
FIG. 4 is a graph showing the effect of different pH on the adsorption of cobalt ions by Bacillus pumilus S1419.
FIG. 5 shows the effect of Bacillus pumilus S1419 on the removal of various ions from a wastewater containing a mimic of the nucleic acid.
FIG. 6 shows the amount of cobalt ions eluted from several cells.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments.
The invention discloses an application of bacillus pumilus in nuclide adsorption or heavy metal ion adsorption, wherein the bacillus pumilus is bacillus pumilus (Bacillus pumilus:)Bacillus pumilus) S1419, which is preserved in the China general microbiological culture Collection center (CGMCC) in 2019, 5 and 16 months, wherein the preservation number is as follows: CGMCC No. 17816.
The invention is directed to nuclides60Co、58Co, and Co2+The adsorption effect of the ions is particularly outstanding.
60Co、58The wastewater of the Co nuclide generally contains chromium ions, manganese ions and iron ions which can be activated besides radioactive cobalt ions, and the Bacillus pumilus S1419 can also be used for treating the heavy metal ions.
The invention discloses a method for treating nuclide wastewater or heavy metal wastewater, which comprises the following steps in detail.
S1. plate activation: inoculating the strain of Bacillus pumilus S1419 to LB solid medium with inoculating loop, and standing in 30 deg.C incubator for 24 hr. The LB solid medium consists of the following components: 10 g/L of peptone, 10 g/L of sodium chloride, 5g/L of yeast extract and 15g/L of agar powder, adjusting the pH value to 6.9-7.1, sterilizing at 121 ℃ for 30min, and cooling to room temperature.
S2, liquid activation: and (3) inoculating a single colony grown from the solid culture medium by using an inoculating loop, inoculating the single colony to an LB liquid culture medium, and performing shake culture at the temperature of 30 ℃ and the rotating speed of 140-150 r/min for 24-25 h to obtain a bacterial liquid. The LB liquid medium consists of the following components: 10 g/L of peptone, 10 g/L of sodium chloride and 5g/L of yeast extract, and adjusting the pH to 6.9-7.1.
And S3, centrifuging the obtained bacterial liquid at 5000-6000 r/min, and centrifuging for 5min at 20 ℃. And collecting the bacillus pumilus S1419 thallus precipitated at the bottom of the centrifuge tube, suspending the thallus obtained by centrifugation in an isometric NaCl solution with the mass fraction of 0.9-1%, washing off substances adhered to the surfaces of the thallus, and repeatedly washing once.
And S4, adding a NaOH solution into the nuclide wastewater or the heavy metal wastewater to adjust the pH value to 5.0-8.1. And (3) resuspending the cleaned thalli into nuclide wastewater or heavy metal wastewater, and adjusting the OD of the bacillus pumilus S1419 in the nuclide wastewater or the heavy metal wastewater to be 1.2-1.3 by using a spectrophotometer. Carrying out adsorption reaction in a temperature control shaking table with the temperature of 25-35 ℃ and the rotating speed of 150-200 r/min.
S5, after the adsorption reaction is carried out for 4-6 min, the treated nuclide wastewater or heavy metal wastewater is centrifuged at 6000-7000 r/min and 5min at 20 ℃ to remove the bacillus pumilus.
To verify the application effect of bacillus pumilus S1419 in nuclide adsorption, the following design experiment was performed.
1. Procedure of experiment
(1) Preparing a reaction solution for simulating nuclide wastewater: 5 mol/L PIPES buffer solution is prepared, and weighed reagents are added into the buffer solution: CoCl2、CrCl3、MnCl4And FeCl3The pH was adjusted to the desired value with a 5 mol/L NaOH solution.
Determining the concentration of the target metal element: and detecting the target metal element by using ICP-OES, and acquiring a standard curve of conversion of a detection signal and concentration according to a series of pre-prepared mixed standard samples with the concentrations of cobalt, chromium, manganese and iron of 0.3, 0.6, 1.0, 2.0, 5.0 and 10.0 mg/L to determine the corresponding concentration of the target metal element.
(2) According to the treatment method, the bacillus pumilus S1419 strain to be used for experiments is activated by a flat plate to observe the colony morphology, then is activated by liquid, centrifuged, washed, and the cleaned thallus is fixed by glutaraldehyde and dehydrated by ethanol and then is observed under a scanning electron microscope.
(3) According to the treatment method, the strain to be tested is cultured under proper conditions, and is washed and then resuspended in the reaction liquid, the initial concentration of the cobalt ions in the adsorbed reaction liquid is 2.5-5.0 mg/L, the initial pH of the reaction liquid is 5.0-8.1, and the adsorption reaction is carried out in a temperature-controlled shaking table with the temperature of 25-35 ℃ and the rotating speed of 150-200 r/min.
Bacillus pumilus S1419 used in the present invention was used as the test strain 4, which was labeled as strain 4 in FIG. 6. Meanwhile, the following three control examples were set to carry out adsorption reactions under the same reaction conditions:
the first comparative example is as follows: using Bacillus subtilis (B), (B)Bacillus subtilis) As an experimental strain 1, the treatment of various heavy metal ions has been reported more. Labeled strain 1 in figure 6.
Comparative example two: using BacillusBacillussp.WS-XY1The gram-positive bacteria separated from the anode chamber of the microbial fuel cell as the experimental strain 2 have electrochemical activity. Labeled strain 2 in fig. 6.
Comparative example three: using Bacillus pumilus (B.) (Bacillus pumilus) S1420 as an experimental strain 3, which was deposited in the common microorganism center of the committee for culture collection of microorganisms of china (No. 1 west way of north chen, haoyang, beijing, institute of microbiology, china academy of sciences) 16/5 in 2019, abbreviated as CGMCC, with the deposition number: CGMCC No. 17817. Labeled strain 3 in figure 6.
The bacterial strain of the invention and the three bacterial strains of the comparative example are respectively activated once by an LB solid culture medium, then a single bacterial colony is selected to be activated for 24-25 h by an LB liquid culture medium, the bacterial strain is collected by centrifugation for 5min at 5000-6000 r/min at 20 ℃, and then NaCl solution with the same volume and mass fraction of 0.9-1% is used for washing off substances adhered to the surface of the bacterial strain. In order to ensure the comparability of the experiment, the cell number of the four strains is regulated to 1.2-1.3 of OD suspended in the reaction solution.
(4) And sampling at different reaction time points (0, 5min,20min,40min and 120 min) in the adsorption reaction process, centrifuging at 6000-7000 r/min and 20 ℃ for 5min, separating the bacillus pumilus S1419, and placing the supernatant sample at 4 ℃ in a dark place. Measuring Co in sample to be measured obtained by adsorption reaction of Bacillus pumilus S14192+Concentration, fig. 2 was obtained. The amount of cobalt ions adsorbed by the cells was calculated and found as shown in FIG. 3.
The initial concentration of cobalt ions in the reaction liquid for adsorption treatment of Bacillus pumilus S1419 was 5.0mg/L, the pH was 5.18, 6.53, and 8.04, the above sampling and detection were performed when the reaction for adsorption of cobalt ions by the mycelia proceeded for 0,5min,20min, 60 min, and 120min, and the adsorption removal ratio of Bacillus pumilus S1419 to cobalt ions was calculated, and FIG. 4 was obtained. Similarly, after the bacillus pumilus S1419 thallus is detected to adsorb and treat the mimic nuclide wastewater for 0min and 120min, the initial Co and the residual Co in the mimic nuclide wastewater2+、Cr3+、 Mn2+And Fe3+ Concentration, difference between the two concentrations, i.e. adsorption of Co by Bacillus pumilus S14192+、Cr3+、Mn2+And Fe3+The contents of the four ions, fig. 5 was obtained.
(5) The initial concentration of Co contained in the adsorbed material is 5mg/L2+Bacillus subtilis (strain 1) after 120min of ionic reaction liquid,Bacillussp.WS-XY1The cells (strain 2), Bacillus pumilus S1420 (strain 3) and Bacillus pumilus S1419 (strain 4) were washed once with 5mmol/L PIPES, then 0.1 mol/L HCl, and the supernatants from the two washes were collected, protected from light, retained at 4 ℃ and eluted to obtain the amount of cobalt ions, as shown in FIG. 6. The interaction of the thalli on cobalt ions is divided into extracellular adsorption and fine entryThe thallus has two aspects. Extracellular adsorption is classified according to the degree of binding tightness: the cobalt ions loosely bound to the bacterial cells can be obtained by washing the adsorbed bacterial cells with PIPES, while the cobalt ions tightly bound to the bacterial cells are obtained by eluting with hydrochloric acid after PIPES elution, and the residual cobalt ion amount is the amount of cobalt ions entering the bacterial cells.
2. Analysis of Experimental results
The strain of the bacillus pumilus S1419 can form two colony forms, namely a semitransparent colony and an opaque colony, after being activated by a flat plate, the generated semitransparent colony is beige, the edge of the semitransparent colony is irregular and serrated, the opaque colony is smaller and smooth, and the middle of the colony is slightly shrunk and protruded; translucent colonies gradually decreased with increasing passage number. As shown in FIG. 1, after Bacillus pumilus S1419 was activated with a liquid, the washed cells were fixed with glutaraldehyde and dehydrated with ethanol, and then observed under a scanning electron microscope to have a short rod shape of about 0.6 to 0.7. mu.m.times.2.0 to 3.0. mu.m.
As can be seen from FIGS. 2 to 4, Bacillus pumilus S1419 can rapidly adsorb low-concentration cobalt ions, and the adsorption balance can be achieved within 3-5 min. For different concentrations of Co2+Ion, Bacillus pumilus S1419 reached equilibrium of adsorption at the first sampling time point, 5min, indicating that the active sites on the surface of Bacillus pumilus S1419 were rapidly available to Co2+And (4) ion reaction. Bacillus pumilus S1419 for Co concentrations of 2.5mg/L and 5mg/L, respectively2+The ion equilibrium adsorption capacity can reach 83.6 mg/g and 174.0 mg/g. When the pH value of the reaction solution is 6.53, the adsorption removal rate of cobalt ions is highest, the adsorption effect is best, the change of the pH value has a slight influence on the adsorption effect of the Bacillus pumilus S1419, but the Bacillus pumilus S1419 can better adsorb the cobalt ions within the pH range of 5.0-8.1.
As can be seen from FIG. 5, the element possibly eluted in the nuclide preparation and application device is mainly Cr3+、Mn2+And Fe3+Three ions, which must ensure that the thallus treatment effect is not interfered by the elements in the treatment of nuclide wastewater, and in the treatment experiment of simulated nuclide wastewater, the bacillus pumilus S1419 is applied to the initial concentrationCo with the degree of 4 mg/L-5 mg/L2+、Cr3+、Mn2+And Fe3+Four ions all have good removal effect, and Cr3+、Mn2+And Fe3+Three ions are not paired with Co2+The removal effect of (2) causes obvious influence, and shows that the bacillus pumilus S1419 can simultaneously remove Co in nuclide wastewater2+、Cr3+、Mn2+、 Fe3+And the like.
As can be seen from FIG. 6, Co not removed by the strain2+The ions remained in the supernatant of the reaction sample solution, and the comparison shows that the Bacillus subtilis (strain 1) has low Co concentration2+The removal effect of the ions is the worst,Bacillussp.WS-XY1(Strain 2) on Low concentration Co2+The removal effect of the ions is slightly improved compared with that of the bacillus subtilis, and the two strains of the bacillus pumilus have low Co concentration2+The ion removal effect is the best. Comparing the removal effect of two strains of Bacillus pumilus, the two strains of Bacillus pumilus can be used for detecting Co2+The site of ion action is mainly located on the surface of the thallus. The removal is mostly extracellular and Co2+Ions are tightly bound, and Bacillus pumilus S1419 and Co are used in the present invention2+The binding of the ion eluted less in PIPES, indicating that it was Co-poor2+The binding effect of the ions is tighter, the desorption is not easy, and the method is more suitable for the adsorption application of nuclide and heavy metal ions.
The above description is only a preferred embodiment of the present invention, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also included in the protection scope of the present invention.
Claims (8)
1. The application of the bacillus pumilus in nuclide adsorption or heavy metal ion adsorption is characterized in that: the bacillus pumilus is bacillus pumilus (B.) (B.)bacillus pumilus) S1419, the strain accession number is: CGMCC No. 17816; the nuclide is60Co or58Co, the heavy metal ion is Co2+Ions.
2. A method for treating nuclide wastewater or heavy metal wastewater is characterized by comprising the following steps: using Bacillus pumilus (B.) (bacillus pumilus) S1419, treating nuclide wastewater or heavy metal wastewater, wherein the nuclide wastewater or the heavy metal wastewater contains Co2+Ions, adsorption of Co in wastewater by Bacillus pumilus S14192+And the strain preservation number of the bacillus pumilus S1419 is as follows: CGMCC No. 17816.
3. The nuclide wastewater or heavy metal wastewater treatment method as set forth in claim 2, characterized in that: the nuclide wastewater or heavy metal wastewater also contains Cr3+、Mn2+、Fe3+One or more of ions.
4. The nuclide wastewater or heavy metal wastewater treatment method as set forth in claim 3, characterized in that: comprises the following steps of (a) carrying out,
s1, inoculating the strain of Bacillus pumilus S1419 to an LB solid culture medium by using an inoculating loop, and performing static culture in a constant temperature incubator at 25-35 ℃ for 24-25 h;
s2, taking a single colony growing from the solid culture medium by using an inoculating loop, inoculating the single colony to an LB liquid culture medium, and performing shake culture for 24-25 h at the temperature of 25-35 ℃ and the rotating speed of 140-160 r/min to obtain a bacterial liquid;
s3, centrifuging or filtering or naturally settling the obtained bacterial liquid, collecting the bacillus pumilus S1419 thallus precipitated at the bottom of a centrifugal tube, suspending the obtained thallus in an NaCl solution with the same volume and the mass fraction of 0.9-1%, washing off substances adhered to the surfaces of the thallus, and repeatedly washing for a plurality of times;
s4, resuspending the cleaned thallus into nuclide wastewater or heavy metal wastewater, and carrying out adsorption reaction in a temperature control shaking table at the temperature of 25-35 ℃ and the rotating speed of 150-200 r/min;
and S5, after the adsorption reaction, removing the bacillus pumilus in the treated nuclide wastewater or heavy metal wastewater through centrifugation, filtration or natural sedimentation.
5. The nuclide wastewater or heavy metal wastewater treatment method as set forth in claim 4, characterized in that: and (3) in the step S4, the thalli are resuspended in the nuclide wastewater or heavy metal wastewater, and the OD of the bacillus pumilus S1419 in the nuclide wastewater or heavy metal wastewater is adjusted to be 1.2-1.3 by a spectrophotometer.
6. The nuclide wastewater or heavy metal wastewater treatment method as set forth in claim 5, characterized in that: said Co2+The initial concentration of the ions is less than or equal to 5 mg/L.
7. The nuclide wastewater or heavy metal wastewater treatment method as set forth in claim 4, characterized in that: and (S3) centrifuging the obtained bacterial liquid at 5000-6000 r/min and 20 ℃ for 5-10 min, and collecting the bacillus pumilus S1419 thallus precipitated at the bottom of the centrifugal tube.
8. The nuclide wastewater or heavy metal wastewater treatment method as set forth in claim 4, characterized in that: before the bacteria are put into the nuclide wastewater or the heavy metal wastewater in the step S4, adding a NaOH solution into the nuclide wastewater or the heavy metal wastewater to adjust the pH to 5.0-8.1; and step S5, removing the bacillus pumilus in the treated nuclide wastewater or heavy metal wastewater after the adsorption reaction time is 3-5 min.
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