CN111420113A - Intelligent breathable dressing with antibacterial effect and preparation method thereof - Google Patents
Intelligent breathable dressing with antibacterial effect and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/80—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special chemical form
- A61L2300/802—Additives, excipients, e.g. cyclodextrins, fatty acids, surfactants
Abstract
The invention belongs to the technical field of medical materials, and relates to an intelligent breathable dressing with a bacteriostatic action and a preparation method thereof.A well-accumulated fiber of medical cotton and a soft hydrogel system based on chitosan silk fibroin are adhered together to prepare a substrate by utilizing a material preparation technology and a material preparation method according to the interface and phase action of the material, tea tree oil is selected as an oil phase, hydroxypropyl- β -cyclodextrin is selected as an embedded body, Tween 80 is selected as an emulsion, and a hydroxypropyl- β -cyclodextrin rod-shaped structure inclusion compound is formed by self-assembly to realize the uniform dispersion of the tea tree oil on an organic matrix.
Description
Technical Field
The invention belongs to the technical field of medical materials, and particularly relates to an intelligent breathable dressing with a bacteriostatic action and a preparation method thereof.
Background
Regenerative treatment of skin defects is a central requirement of current tissue engineering. According to the world health organization, it is estimated that 18 million people die of burns every year worldwide, most of which occur in low-income and medium-income countries. The skin, the largest tissue of the human body, is a barrier for maintaining the internal environment stable and preventing the invasion of microorganisms, and plays important roles in thermoregulation, fluid balance, immune monitoring and self-wound healing. However, the problem of wound infection due to metabolic diseases such as trauma, bacterial infection, burns, tumors, and diabetes is becoming serious, and even serious patients may cause life risks. With the accelerated industrialization process in China, the incidence rate of car accidents and burn accidents gradually increases every year, and the base number of patients needing transplantation is large. The report indicates that the clinical requirement of the skin repair material in China is huge, the current market value is more than 250 hundred million yuan, and the annual growth rate is more than 15 percent; the number of patients with burn occupying the hospital is 1 st; transplantation also accounts for the 1 st frequency of tissue transplantation.
The medical cotton is composed of fibrin, the fibrin has a certain function of antibacterial binding matrix, and the compact structure among protein fibers is similar to that of natural silkworm cocoons. Chitosan (Chitosan, CS) consists of the polymerization of glucosamine and N-acetylglucosamine, which are integral parts of mammalian tissues. It is a non-toxic, biocompatible and biodegradable polymer. The existence of a large amount of active-NH on the molecular chain of CS2and-OH, which can be modified by introducing some new groups, so the properties of chitosan are very plastic. CS has excellent biological properties such as good biocompatibility, blood compatibility and the like, and can be prepared into films and drug-loaded coagulantsGlue or ball as the carrier material for slow release of medicine. Silk Fibroin (SF) consists of a protein-based biomacromolecule consisting of 5507 amino acid repeats, consisting of a (Gly-Ala-Gly-Ser) n repeat. It is applied to clinical research in the forms of scaffold, hydrogel, thin film fiber, microsphere, etc. The silk fibroin has good physicochemical property and mechanical property, good water vapor permeability and biodegradability. SF is used as a cell culture carrier, has good biocompatibility and can support the adhesion of cells. SF protein molecules are mutually folded and communicated, which is favorable for transporting cell nutrients and improving the metabolism level of organisms, and is widely applied to various fields of cosmetics, medicines and the like. Tea Tree Oil (TTO) is a purely natural plant essential oil extracted from melaleuca alternifolia leaves by distillation. It is a complex mixture of about 100 different compounds, the main components being monoterpenes and the corresponding alcohols. TTO has a variety of pharmaceutical properties, including antibacterial, antiviral, anti-inflammatory, and analgesic properties. In recent years, it has been widely used as a topical antibacterial agent, such as wound infections, bacterial fungal infections and oral mucosa and blisters and vaginitis.
Patent CN108950713A discloses a method for preparing an antibacterial durable stable viscose fiber material, wherein β -cyclodextrin and tea tree oil are added into a common viscose spinning solution in a microcapsule form, and wet spinning is carried out to uniformly distribute an inclusion compound in the common viscose spinning solution, so that in the using process, tea tree oil is slowly released and can moisturize the skin on one hand, and the bacteriostatic action of the tea tree oil is fully reflected on the other hand, so that the viscose fiber has a good health-care effect.
The invention utilizes the technology and the method of material preparation according to the interface and phase action of the material, designs the component composition, the structural arrangement and the attribute characteristics of the material to be close to human tissues to the greatest extent, so that the material has a better topological structure, more truly simulates the steady-state environment that the human native tissues are mutually connected and interdependent, and the material has the best bioactivity and compatibility.
Disclosure of Invention
The invention aims to provide an intelligent breathable dressing for bacterial infection, wherein a well-accumulated medical cotton fiber and a soft hydrogel system based on chitosan silk fibroin are bonded together to prepare a substrate, and a hydroxypropyl- β -cyclodextrin inclusion compound is formed by self-assembly to realize the uniform dispersion of tea tree oil on an organic matrix, so that the intelligent breathable dressing with an antibacterial effect is prepared in a bionic manner.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing an intelligent breathable dressing with an antibacterial effect comprises the following steps of bonding well-accumulated medical cotton fibers and a soft hydrogel system based on chitosan silk fibroin together to prepare a substrate by utilizing a material preparation technology and a material preparation method according to the interface and phase effects of materials, forming a hydroxypropyl- β -cyclodextrin inclusion compound by self-assembly to realize uniform dispersion of tea tree oil on an organic matrix, and performing bionic preparation on the intelligent breathable dressing with the antibacterial effect, wherein the preparation method specifically comprises the following steps:
the method comprises the following steps:
(1) dissolving silk fibroin in 10ml of deionized water;
(2) after dissolving under magnetic stirring, adding chitosan, fully stirring to uniformly mix the chitosan and the silk fibroin to obtain a substrate solution of chitosan and silk fibroin, and adding a proper amount of acetic acid to make the concentration of the acetic acid be 1 wt%;
(3) dissolving hydroxypropyl- β -cyclodextrin HP- β -CD in deionized water, and treating for 5min with a vortex machine to mix uniformly;
(4) adding tea tree oil into the HP- β -CD solution prepared in the step (3), magnetically stirring for 2h to enable an organic external phase to uniformly wrap the tea tree oil to obtain an oil-in-water tea tree oil primary emulsion, adding Tween 80 into the obtained HP- β -CD primary emulsion under an ultrasonic condition, treating for 10min by using a vortex machine, and carrying out water bath reaction to obtain the HP- β -CD emulsion carried by the tea tree oil;
(5) adding the solution obtained in the step (4) into the solution obtained in the step (2) under the ultrasonic condition, and carrying out ultrasonic treatment for 5min by using a homogenizer;
(6) cutting medical cotton into 2cm round pieces with a mass of 0.1 g;
(7) uniformly soaking 2ml of the product obtained in the step (5) on the medical cotton obtained in the step (6), and drying;
(8) and (5) freeze-drying the product obtained in the step (7) to obtain the medical dressing.
Further, in the step (2), the mass ratio of chitosan to silk fibroin is 1: 1.
Preferably, the mass of the silk fibroin is 0.1-0.2g, and the mass of the chitosan is 0.1-0.2 g.
Further, in the step (3), the concentration of hydroxypropyl- β -cyclodextrin (HP- β -CD) was 100 wt%.
Preferably, 0.8-1.0 g of hydroxypropyl- β -cyclodextrin (HP- β -CD) is dissolved in 0.8-1.0 ml of deionized water.
Further, in the step (4), the concentration of the tween 80 in the tea tree oil primary emulsion is 1wt% -2 wt%.
Preferably, the dosage of the Tween 80 is 0.01-0.02 g.
Further, in the step (4), the mass ratio of the HP- β -CD solution to the tea tree oil is 10:1
Preferably, the amount of the tea tree oil is 0.1-0.2 ml.
Further, in the step (4), the water bath reaction specifically comprises: reacting in water bath at 45 ℃ for 12-24 h.
Further, in the step (4), the tea tree oil is obtained by extracting leaves of melaleuca alternifolia.
Further, in the step (7), the drying is specifically oven drying at 60 ℃ for 12 h.
An intelligent breathable dressing with antibacterial effect prepared by the method.
The invention has the following remarkable advantages:
(1) the material selected by the invention has good biocompatibility and air permeability, is used with a natural product delivery system to realize slow release and interface phase separation, does not influence skin respiration while resisting external bacterial infection, and induces skin regeneration.
(2) The invention bonds a piece of well-accumulated medical cotton fiber and a soft hydrogel system based on chitosan silk fibroin together to prepare a substrate, self-assembles to form a hydroxypropyl- β -cyclodextrin inclusion compound to realize the uniform dispersion of tea tree oil on an organic matrix, and bionically prepares the intelligent breathable dressing with the bacteriostatic action.
(3) The preparation method has the advantages of simple preparation process, convenient operation and low cost.
Drawings
Fig. 1 is an SEM image (1000 ×) of the smart breathable dressing with bacteriostatic effect prepared in example 3.
Fig. 2 is an SEM image (5000 ×) of the intelligent breathable dressing with bacteriostatic effect prepared in example 3.
Fig. 3 is a test chart of the inhibition zone of the co-culture of the intelligent breathable dressing with the antibacterial effect prepared in example 3, escherichia coli and staphylococcus aureus.
Detailed Description
In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the following examples are only examples of the present invention and do not represent the scope of the present invention defined by the claims.
Example 1
A preparation method of an intelligent breathable dressing with a bacteriostatic effect comprises the following steps:
(1) dissolving 0.2g silk fibroin in 10ml deionized water;
(2) after dissolving under magnetic stirring, adding 0.2g of chitosan, fully stirring to uniformly mix the chitosan and the silk fibroin to obtain a substrate solution of chitosan and silk fibroin, and adding a proper amount of acetic acid to make the concentration of the acetic acid be 1 wt%;
(3) dissolving 0.8g hydroxypropyl- β -cyclodextrin (HP- β -CD) in 0.8ml deionized water, and treating with vortex machine for 5min to mix;
(4) adding 0.1ml of tea tree oil solution into the saturated HP- β -CD solution prepared in the step (3), magnetically stirring for 2h to enable an organic external phase to uniformly wrap the tea tree oil to obtain an oil-in-water tea tree oil primary emulsion, slowly dripping 0.01g of Tween 80 into the obtained HP- β -CD primary emulsion under the ultrasonic condition, treating for 10min by using a vortex machine, and reacting in a water bath at 45 ℃ for 24h to obtain the HP- β -CD emulsion carried by the tea tree oil;
(5) slowly dripping the solution obtained in the step (4) into the solution obtained in the step (2) under the ultrasonic condition, and carrying out ultrasonic treatment for 5min by a homogenizer;
(6) cutting medical cotton into 2cm round pieces with a mass of 0.1 g;
(7) uniformly coating 2ml of the product obtained in the step (5) on the medical cotton in the step (6), and drying in a 60 ℃ oven for 12h until the medical cotton is fully absorbed;
(8) and (5) freeze-drying the product obtained in the step (7) to obtain the breathable dressing.
Example 2
A preparation method of an intelligent breathable dressing with a bacteriostatic effect comprises the following steps:
(1) dissolving 0.2g silk fibroin in 10ml deionized water;
(2) after dissolving under magnetic stirring, adding 0.2g of chitosan, fully stirring to uniformly mix the chitosan and the silk fibroin to obtain a substrate solution of chitosan and silk fibroin, and adding a proper amount of acetic acid to make the concentration of the acetic acid be 1 wt%;
(3) dissolving 0.9g hydroxypropyl- β -cyclodextrin (HP- β -CD) in 0.9ml deionized water, and treating with vortex machine for 5min to mix;
(4) adding 0.1ml of tea tree oil solution into the saturated HP- β -CD solution prepared in the step (3), magnetically stirring for 2h to enable an organic external phase to uniformly wrap the tea tree oil to obtain an oil-in-water tea tree oil primary emulsion, slowly dripping 0.01g of Tween 80 into the obtained HP- β -CD primary emulsion under the ultrasonic condition, treating for 10min by using a vortex machine, and reacting in a water bath at 45 ℃ for 24h to obtain the HP- β -CD emulsion carried by the tea tree oil;
(5) slowly dripping the solution obtained in the step (4) into the solution obtained in the step (2) under the ultrasonic condition, and carrying out ultrasonic treatment for 5min by a homogenizer;
(6) cutting medical cotton into 2cm round pieces with a mass of 0.1 g;
(7) uniformly coating 2ml of the product obtained in the step (5) on the medical cotton in the step (6), and drying in a 60 ℃ oven for 12h until the medical cotton is fully absorbed;
(8) and (5) freeze-drying the product obtained in the step (7) to obtain the breathable dressing.
Example 3
A preparation method of an intelligent breathable dressing with a bacteriostatic effect comprises the following steps:
(1) dissolving 0.2g silk fibroin in 10ml deionized water;
(2) after dissolving under magnetic stirring, adding 0.2g of chitosan, fully stirring to uniformly mix the chitosan and the silk fibroin to obtain a substrate solution of chitosan and silk fibroin, and adding a proper amount of acetic acid to make the concentration of the acetic acid be 1 wt%;
(3) dissolving 1.0g hydroxypropyl- β -cyclodextrin (HP- β -CD) in 1.0ml deionized water, and treating for 5min with a vortex machine to mix well;
(4) adding 0.1ml of tea tree oil solution into the saturated HP- β -CD solution prepared in the step (3), magnetically stirring for 2h to enable an organic external phase to uniformly wrap the tea tree oil to obtain an oil-in-water tea tree oil primary emulsion, slowly dripping 0.01g of Tween 80 into the obtained HP- β -CD primary emulsion under the ultrasonic condition, treating for 10min by using a vortex machine, and reacting in a water bath at 45 ℃ for 24h to obtain the HP- β -CD emulsion carried by the tea tree oil;
(5) slowly dripping the solution obtained in the step (4) into the solution obtained in the step (2) under the ultrasonic condition, and carrying out ultrasonic treatment for 5min by a homogenizer;
(6) cutting medical cotton into 2cm round pieces with a mass of 0.1 g;
(7) 9, uniformly coating 2ml of the product obtained in the step (5) on the medical cotton in the step (6), and drying in a 60 ℃ drying oven for 12 hours until the medical cotton is fully absorbed;
(8) and (5) freeze-drying the product obtained in the step (7) to obtain the medical dressing.
An SEM image (1000 ×) of the intelligent breathable dressing with the bacteriostatic action prepared in the embodiment is shown in figure 1. as can be seen from figure 1, the surface of the dressing is rough, and HP- β -CD is orderly stretched on the surface of medical cotton, so that the promotion of cell adhesion in wound repair is facilitated.
In the example, an SEM picture (5000 ×) of the prepared intelligent breathable dressing with the bacteriostatic action is shown in figure 2. as can be seen from figure 2, tea tree oil is wrapped by HP- β -CD, and the HP- β -CD clathrate compound is successfully prepared.
Test of the bacteriostatic zone of the co-culture of the intelligent breathable dressing and bacteria:
respectively preparing bacterial suspension with concentration of 108CFU/ml gram-negative bacteria (G)¯) Escherichia coli (E.coli) And gram-positive bacteria (G)+) Staphylococcus aureus (1)S.aures) Preparing agar plates, dripping 0.1ml of bacteria liquid after each agar plate is solidified, and uniformly coating the bacteria liquid, and then uniformly coating the bacteria inhibiting dressing (1.5 × 1.5.5 cm)2) Placing the mixture in a culture dish, culturing for 24 hours at 37 ℃, observing the size of a bacteriostatic zone, and counting data. The results are shown in FIG. 3. As can be seen from figure 3, after 24 hours, no matter escherichia coli or staphylococcus aureus, an obvious bacteriostatic circle can be observed, and the good bacteriostatic property of the intelligent breathable dressing is proved.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (10)
1. A preparation method of an intelligent breathable dressing with a bacteriostatic action is characterized in that well-accumulated medical cotton fibers and a soft hydrogel system based on chitosan silk fibroin are bonded together to prepare a substrate, a hydroxypropyl- β -cyclodextrin rod-shaped structure inclusion compound is formed by self-assembly to realize uniform dispersion of tea tree oil on an organic matrix, and the intelligent breathable dressing with the bacteriostatic action is prepared in a bionic manner.
2. The preparation method of the intelligent breathable dressing with the bacteriostatic action according to claim 1, characterized in that: the method comprises the following steps:
(1) dissolving silk fibroin in 10ml of deionized water;
(2) after dissolving under magnetic stirring, adding chitosan, fully stirring to uniformly mix the chitosan and the silk fibroin to obtain a substrate solution of chitosan and silk fibroin, and adding a proper amount of acetic acid to make the concentration of the acetic acid be 1 wt%;
(3) dissolving hydroxypropyl- β -cyclodextrin HP- β -CD in deionized water, and treating for 5min with a vortex machine to mix uniformly;
(4) adding tea tree oil into the HP- β -CD solution prepared in the step (3), magnetically stirring for 2h to enable an organic external phase to uniformly wrap the tea tree oil to obtain an oil-in-water tea tree oil primary emulsion, adding Tween 80 into the obtained HP- β -CD primary emulsion under an ultrasonic condition, treating for 10min by using a vortex machine, and carrying out water bath reaction to obtain the HP- β -CD emulsion carried by the tea tree oil;
(5) adding the solution obtained in the step (4) into the solution obtained in the step (2) under the ultrasonic condition, and carrying out ultrasonic treatment for 5min by using a homogenizer;
(6) cutting medical cotton into 2cm round pieces with a mass of 0.1 g;
(7) uniformly soaking 2ml of the product obtained in the step (5) on the medical cotton obtained in the step (6), and drying;
(8) and (5) freeze-drying the product obtained in the step (7) to obtain the medical dressing.
3. The method of claim 2, wherein: in the step (2), the mass ratio of the chitosan to the silk fibroin is 1: 1.
4. The process according to claim 2, wherein the concentration of hydroxypropyl- β -cyclodextrin HP- β -CD in the step (3) is 100% by weight.
5. The method of claim 2, wherein: in the step (4), the concentration of the Tween 80 in the tea tree oil primary emulsion is 1wt% -2 wt%.
6. The method according to claim 2, wherein the ratio of the HP- β -CD solution to the tea tree oil in step (4) is 10:1 by mass.
7. The method of claim 2, wherein: in the step (4), the water bath reaction specifically comprises the following steps: reacting in water bath at 45 ℃ for 12-24 h.
8. The method of claim 2, wherein: in step (4), the tea tree oil is obtained by extracting leaves of melaleuca alternifolia.
9. The method of claim 2, wherein: in the step (7), the drying is specifically drying in an oven at 60 ℃ for 12 h.
10. The intelligent breathable dressing with bacteriostatic action prepared by the preparation method of any one of claims 1-9.
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| CN101138648A (en) * | 2006-09-07 | 2008-03-12 | 电子科技大学中山学院 | Method of preparing antimicrobial hemostasis Chinese medicine-containing biological dressings |
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| CN108836635A (en) * | 2016-02-03 | 2018-11-20 | 丁永新 | anti-inflammatory antibacterial medical dressing |
| CN108950713A (en) * | 2018-05-15 | 2018-12-07 | 钱兴 | A kind of preparation method of the durable stable type viscose material of antibacterial |
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2020
- 2020-05-19 CN CN202010423226.8A patent/CN111420113A/en active Pending
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| CN101138648A (en) * | 2006-09-07 | 2008-03-12 | 电子科技大学中山学院 | Method of preparing antimicrobial hemostasis Chinese medicine-containing biological dressings |
| CN102120043A (en) * | 2011-02-23 | 2011-07-13 | 中国人民解放军军事医学科学院基础医学研究所 | Absorbent cotton gauze containing nanometer silver and chitosan, preparation device as well as method thereof |
| CN108836635A (en) * | 2016-02-03 | 2018-11-20 | 丁永新 | anti-inflammatory antibacterial medical dressing |
| CN108950713A (en) * | 2018-05-15 | 2018-12-07 | 钱兴 | A kind of preparation method of the durable stable type viscose material of antibacterial |
| CN110478391A (en) * | 2019-09-27 | 2019-11-22 | 福州大学 | A kind of tea oil microballoon and preparation method thereof with bacteriostasis |
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Application publication date: 20200717 |