CN111419204A - Improved pressure volume catheter experiment method - Google Patents
Improved pressure volume catheter experiment method Download PDFInfo
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- CN111419204A CN111419204A CN202010396583.XA CN202010396583A CN111419204A CN 111419204 A CN111419204 A CN 111419204A CN 202010396583 A CN202010396583 A CN 202010396583A CN 111419204 A CN111419204 A CN 111419204A
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- China
- Prior art keywords
- carotid artery
- catheter
- common carotid
- volume
- head end
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/02—Detecting, measuring or recording pulse, heart rate, blood pressure or blood flow; Combined pulse/heart-rate/blood pressure determination; Evaluating a cardiovascular condition not otherwise provided for, e.g. using combinations of techniques provided for in this group with electrocardiography or electroauscultation; Heart catheters for measuring blood pressure
- A61B5/02028—Determining haemodynamic parameters not otherwise provided for, e.g. cardiac contractility or left ventricular ejection fraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/02—Detecting, measuring or recording pulse, heart rate, blood pressure or blood flow; Combined pulse/heart-rate/blood pressure determination; Evaluating a cardiovascular condition not otherwise provided for, e.g. using combinations of techniques provided for in this group with electrocardiography or electroauscultation; Heart catheters for measuring blood pressure
- A61B5/024—Detecting, measuring or recording pulse rate or heart rate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/02—Detecting, measuring or recording pulse, heart rate, blood pressure or blood flow; Combined pulse/heart-rate/blood pressure determination; Evaluating a cardiovascular condition not otherwise provided for, e.g. using combinations of techniques provided for in this group with electrocardiography or electroauscultation; Heart catheters for measuring blood pressure
- A61B5/026—Measuring blood flow
- A61B5/029—Measuring or recording blood output from the heart, e.g. minute volume
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6846—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
- A61B5/6847—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive mounted on an invasive device
- A61B5/6852—Catheters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
Abstract
The invention discloses an improved pressure volume catheter experiment method, which adopts a catheter insertion mode that a catheter and a blood vessel are ligated after a catheter receptor enters a common carotid artery. Specifically, wear two silk threads from the common carotid artery under, a line that gets to be close to the head end is tied up and is tied up the common carotid artery head end, loose slipknot is made to another line, the artery presss from both sides the nearly heart end that closes the carotid artery, cut a osculum on the nearly heart end of common carotid artery head end line ligature department, insert pressure volume pipe through this incision along the reverse blood flow direction of carotid artery indoor to the left, after pipe pressure and volume sensor all got into the common carotid artery, tight loose slipknot is tied up near the head end, bind the pipe that will insert the common carotid artery with the common carotid artery vascular wall, loosen the artery and press from both sides, the pipe is smooth, continue to insert to the ventricle. The invention solves the problems that although the catheter enters the blood vessel in the traditional method, the blood leakage is generated when the catheter is thinner than the blood vessel, and the blood loss time is too long due to unskilled operation or the fact that the individual difference intubation of the experimental animal cannot be quickly inserted into the ventricle.
Description
Technical Field
The invention relates to an experimental method, in particular to an improved pressure-volume catheter experimental method.
Background
The recently developed pressure-volume catheter system is a preload-independent method for detecting the change of systolic and diastolic function, and has become one of the standard methods for evaluating the function of an experimental animal in vivo L V overseas, however, the measurement procedure of the pressure-volume relationship of L V is complicated and has high technical requirements, thereby limiting the application of the method in domestic basic medical research, so far, a plurality of existing technologies for rat cardiac function measurement, such as echocardiography, magnetic resonance, L angendorff perfusion system and micro fluid pressure limiting device are developed as preload-independent methods, the pressure-volume catheter system can still measure the pressure-volume signal of a rat heart, the pressure-volume signal of the rat is still capable of measuring the instantaneous pressure-volume signal, and the instantaneous pressure-volume signal of the rat is still less than the instantaneous pressure-volume signal of the rat, and the final pressure-volume signal of the rat is estimated by using the pressure-volume curve, the final pressure-volume curve of the rat is estimated by the initial pressure-volume curve, the initial pressure-volume curve of the rat, the rat is estimated by the initial pressure-volume curve, the initial curve of the pressure-volume curve of the heart-volume curve, the curve of the heart-volume curve, the curve of the curve.
The existing pressure volume catheter intubation method is generally carried out in a mode that a catheter enters a left ventricle and then is ligated, due to the existence of an arterial valve, the catheter cannot be directly inserted into the left ventricle from an arterial valve opening in most cases, and multiple times of debugging are needed, so that bleeding and blood leakage of the artery occur from the time when the catheter enters a common carotid artery to the time when the catheter is inserted into the ventricle. Some trials have used silk to block the common carotid blood flow, but before cannulation to the ventricle, there was still time to block due to the cannulation requirement.
Disclosure of Invention
In order to solve the problems, the invention provides an improved pressure volume catheter experiment method, which avoids bleeding in the intubation process, thereby avoiding that data caused by blood loss cannot reflect the heart function in the original state and ensuring the accuracy of the data.
In order to achieve the purpose, the invention adopts the technical scheme that:
an improved pressure-volume catheter experiment method is characterized in that a catheter receptor is not required to be ligated until the catheter receptor completely enters the left ventricle through the common carotid artery, but a catheter insertion mode that the catheter and a blood vessel are ligated after the catheter receptor enters the common carotid artery is adopted. The non-bleeding of the intubation process is realized. Specifically, during the experiment, wear two silk threads from the common carotid artery, get one of them and tie common carotid artery head end near the line of head end, loose slipknot is made to another line, the artery presss from both sides the nearly heart end that closes the carotid artery, cut a osculum on the nearly heart end of common carotid artery head end line ligature department, insert pressure volume pipe along the reverse blood flow direction of carotid artery indoor left through this incision, after pipe pressure and volume sensor all got into the common carotid artery, tight slipknot is tightened to the end near the head, bind pipe and the common carotid artery vascular wall that will insert the common carotid artery, loosen the artery and press from both sides, the pipe is smooth, can continue to insert to the ventricle.
The invention solves the problems that although the catheter is inserted into the blood vessel in the traditional method, the blood leakage is generated because the catheter is thinner than the blood vessel, and the blood loss time is too long because the operation is not skilled or the individual difference intubation of the experimental animal cannot be quickly inserted into the ventricle. The method has wide application range, and all experiments of applying the pressure volume catheter to perform the hemodynamics measurement of the small animals can use or refer to the method for reducing the blood loss problem in the intubation process.
Drawings
FIG. 1 is a schematic diagram of an improved pressure-volume catheter test method according to an embodiment of the present invention;
in the figure: 1-head end silk thread ligation point; 2-incision; 3, binding and fixing points of the catheter and the vessel wall; 4-volume sensor electrode i; 5-a pressure sensor; 6-pressure volume conduit body; 7-volume sensor electrode ii; 8-artery clamp.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention. The embodiment of the invention provides an improved pressure volume catheter experiment method, in the method, the catheter receptor is not required to be ligated until the receptor enters the left ventricle through the common carotid artery, but the catheter and the blood vessel are ligated in a cannula mode after the receptor enters the common carotid artery. Specifically, as shown in fig. 1, during the experiment, two silk threads are threaded from the lower part of the common carotid artery, one of the silk threads close to the head end is taken to tie the head end of the common carotid artery, the other silk thread is tied to a loose slipknot, the proximal end of the carotid artery is clamped by an artery clamp, a small opening is cut at the proximal end of the tying part of the head end line of the common carotid artery, a pressure volume catheter is inserted into the left chamber along the reverse blood flow direction of the common carotid artery through the opening, after the catheter pressure and volume sensors completely enter the common carotid artery, the loose slipknot is tied close to the head end, the catheter inserted into the common carotid artery is tied with the wall of the common carotid artery, the artery clamp is loosened, the catheter is smooth, and the catheter can be.
Examples
Rats were weighed, anesthetized with 3% sodium pentobarbital (40 mg ∙ kg-1 ip), and fixed to the operating table in the supine position. Sterilizing neck skin preparation, making incision in the middle of neck, and separating tracheal cannula. Pressure scaling of the pressure-volume conduit. Separating the right common carotid artery, threading two 4-0 silk threads from the lower part of the common carotid artery, and taking one thread close to the head end to tie the head end of the common carotid artery. The other thread is tied into a loose slipknot. The arterial clamp closes the proximal end of the carotid artery. A small opening is cut at the proximal end of the ligation site of the head end line of the common carotid artery, and a pressure volume catheter is inserted into the left ventricle along the reverse blood flow direction of the carotid artery through the opening. When the pressure and volume sensors of the catheter completely enter the common carotid artery, the loose slipknot is tightened at the end close to the head, and the catheter inserted into the common carotid artery is bound with the wall of the common carotid artery. The arterial clamp is loosened. The catheter is smooth and can be continuously inserted into the ventricle, and the catheter is judged to enter the ventricle according to the pressure waveform change of the physiological recorder. The left ventricular pressure-volume waveform of the anesthetized rat is recorded in real time by using Chart7 software, and the basic curve is recorded for 15 min. Disinfecting the abdominal skin, performing an incision in the middle of the abdomen, quickly closing the inferior vena cava, and recording the change of the waveform of the change of the heart preload. 20 μ l of 30% NaCl solution was injected rapidly from the anterior jugular vein and the pressure-volume waveform change was recorded after injection of hypertonic saline. Heparin anticoagulation blood drawing is carried out, and manual calibration is carried out after the calibration cavity of the calibration kit is filled. A Chart7 software P-V module is applied to draw a pressure volume ring in real time with pressure as the Y axis and volume as the X axis. It can be determined that: heart rate, stroke volume, cardiac output, left ventricular contraction/end diastole pressure, left ventricular contraction/end diastole volume, left ventricular development pressure, dp/dtmax, -dp/dtmin ejection fraction, Tau value, arterial elasticity, stroke work, cardiac efficiency, left ventricular contraction/end diastole pressure volume relation curve slope and other parameters reflecting systolic and diastolic functions, cardiac work and the like.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (2)
1. An improved pressure-volume catheter test method, characterized by: the intubation method is that the catheter and the blood vessel are ligated after the catheter receptor enters the common carotid artery.
2. An improved pressure volume catheter assay method as claimed in claim 1, wherein: during the experiment, wear two silk threads from the common carotid artery, get one of them and tie the common carotid artery head end near the head end, another line is made a loose slipknot, the artery presss from both sides the nearly heart end that closes the carotid artery, cut a osculum on the nearly heart end of common carotid artery head end line ligature department, insert pressure volume pipe through this incision along the reverse blood flow direction of carotid artery indoor to the left, after pipe pressure and volume sensor all got into the common carotid artery, tie loose slipknot near the head end, bind the pipe that will insert the common carotid artery with the common carotid artery vascular wall, loosen the artery and press from both sides, the pipe is smooth, can continue to insert toward the ventricle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010396583.XA CN111419204A (en) | 2020-05-12 | 2020-05-12 | Improved pressure volume catheter experiment method |
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| CN202010396583.XA CN111419204A (en) | 2020-05-12 | 2020-05-12 | Improved pressure volume catheter experiment method |
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| CN111419204A true CN111419204A (en) | 2020-07-17 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002143109A (en) * | 2000-11-13 | 2002-05-21 | Natl Space Development Agency Of Japan | Telemetry system for simultaneous and continuous measurement of ventricular volume and ventricular pressure to be embedded in small animal |
| JP2005253834A (en) * | 2004-03-15 | 2005-09-22 | National Cardiovascular Center | Ventricular volume measuring apparatus and ventricular volume measuring method |
| CN103230308A (en) * | 2013-04-23 | 2013-08-07 | 新乡医学院 | Repeated arterial catheterization method |
| US20140236492A1 (en) * | 2010-08-12 | 2014-08-21 | Heartflow, Inc. | Method and system for patient-specific modeling of blood flow |
| CN107260147A (en) * | 2017-08-02 | 2017-10-20 | 昆明医科大学 | A kind of measuring method of tree shrew blood pressure |
-
2020
- 2020-05-12 CN CN202010396583.XA patent/CN111419204A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002143109A (en) * | 2000-11-13 | 2002-05-21 | Natl Space Development Agency Of Japan | Telemetry system for simultaneous and continuous measurement of ventricular volume and ventricular pressure to be embedded in small animal |
| JP2005253834A (en) * | 2004-03-15 | 2005-09-22 | National Cardiovascular Center | Ventricular volume measuring apparatus and ventricular volume measuring method |
| US20140236492A1 (en) * | 2010-08-12 | 2014-08-21 | Heartflow, Inc. | Method and system for patient-specific modeling of blood flow |
| CN103230308A (en) * | 2013-04-23 | 2013-08-07 | 新乡医学院 | Repeated arterial catheterization method |
| CN107260147A (en) * | 2017-08-02 | 2017-10-20 | 昆明医科大学 | A kind of measuring method of tree shrew blood pressure |
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Application publication date: 20200717 |