CN111398575B

CN111398575B - Wheel disc type immunohistochemical instrument

Info

Publication number: CN111398575B
Application number: CN202010224450.4A
Authority: CN
Inventors: 郭金城; 吴纯; 宋砚明; 孙晓磊; 刘岩
Original assignee: Suzhou Baidao Medical Technology Co ltd
Current assignee: Suzhou Baidao Medical Technology Co ltd
Priority date: 2020-03-26
Filing date: 2020-03-26
Publication date: 2023-04-11
Anticipated expiration: 2040-03-26
Also published as: CN111398575A

Abstract

The invention relates to the technical field of immunohistochemistry, in particular to a wheel disc type immunohistochemical instrument which comprises a workbench, a wheel disc transport mechanism, a constant temperature and humidity box, a reagent tank assembly and a liquid adding mechanism, wherein the wheel disc transport mechanism comprises a support arranged in the workbench, a wheel disc assembly rotatably arranged on the support and a plurality of supporting plate assemblies used for bearing a slide module. The wheel disc transport mechanism is placed in the constant temperature and humidity box to can carry out the liquid feeding to all slide modules through the liquid feeding opening, thereby can be in the in-process of carrying out periodic transportation to layer board subassembly, accomplish simultaneously and carry out the liquid feeding to all slide modules (the liquid feeding action can carry out many times in a plurality of rotation periods, the reagent of liquid feeding at every turn also can be different) and incubate all slide modules. And moreover, the wheel disc transfer mechanism can reduce the space occupation of the upper table surface of the workbench, and the treatment flux can be greatly improved on the premise of not increasing the volume of the whole machine.

Description

Wheel disc type immunohistochemical instrument

Technical Field

The invention relates to the technical field of immunohistochemistry, in particular to a wheel disc type immunohistochemical instrument.

Background

The immunohistochemical instrument is an immunohistochemical staining system which can complete a large flux from deparaffinization to mounting on a machine. The quantity of the existing machines on the market is about 30-40, but the experimental quantity of most actual users exceeds the quantity of the maximum load of the machines, the daily quantity of some users is even as much as 1000, and one or more machines can not meet the actual requirements at home and abroad.

The slide arrangement mode of the current immunohistochemical instrument is not favorable for utilizing the space inside the instrument, if the flux is increased, the volume of the instrument can be only increased, and the practical condition does not allow the volume of the instrument to be overlarge. The glass slide layout on the existing machine is mostly a plane layout or a vertical frame layout, the plane layout is that the glass slides are directly placed on the corresponding positions of the independent working units one by one manually, and then the glass slides are taken one by one after the completion; the vertical rack layout adopts the glass rack, manually places the glass slides on the corresponding positions of the glass rack one by one, and then inserts the glass rack into the clamping groove. Both methods are complicated, and the space of the instrument cannot be fully utilized, so that the occupied area is large, and high flux cannot be realized.

In addition, when a machine on the market is used for carrying out an experiment, the primary antibody reagent is generally taken at any time, the primary antibody reagent needs to be taken out and put back to a storage place (a 4-degree refrigerator) before and after the experiment, and the searching of the antibody is very troublesome in the operation process.

Disclosure of Invention

Technical problem to be solved

The invention mainly aims to provide a wheel disc type immunohistochemical instrument, and aims to solve the problems of small experimental flux and low automation degree of the conventional machine.

(II) technical scheme

In order to achieve the above object, the present invention provides a wheel disk immunohistochemical apparatus comprising:

a work table;

the wheel disc transferring mechanism comprises a support arranged in the workbench, a wheel disc assembly rotatably arranged on the support and a plurality of supporting plate assemblies for bearing the slide module; the wheel disc assembly comprises a shell rotatably mounted on the bracket, a gear assembly and a central connecting rod, wherein the gear assembly is mounted on the shell and comprises a plurality of gear sets;

the gear set comprises a center fixed gear, a plurality of transition gears and transmission gears which are sequentially arranged, and adjacent gears in the gear set are meshed with each other; the central fixed gear, the transition gear and the transmission gear are rotationally connected with the shell, the central fixed gear is fixedly connected with the central connecting rod, and the central connecting rod is fixed on the bracket; each of said gear sets on the same said housing sharing one of said center fixed gears;

the bracket is provided with a driving mechanism for driving the shell to rotate; the supporting plate components are fixedly connected with the transmission gear, and in the rotating process of the shell, included angles between all the supporting plate components and the horizontal plane are kept unchanged;

the supporting plate assemblies are arranged on the wheel disc assembly at intervals and can rotate around the central axis of the wheel disc assembly along with the wheel disc assembly, and the included angles between all the supporting plate assemblies and the horizontal plane are kept unchanged in the rotating process of the wheel disc assembly;

the constant temperature and humidity box is arranged in the workbench, and the wheel disc transport mechanism is accommodated in the constant temperature and humidity box so as to incubate the slide module on the wheel disc transport mechanism; a liquid feeding opening corresponding to the top of the wheel disc transferring mechanism is formed in the table top of the workbench;

the reagent tank assembly is arranged on the workbench, and a plurality of reagent bottles for storing reagents and a refrigerating unit for refrigerating the reagent bottles are arranged in the reagent tank assembly;

the liquid adding mechanism is positioned above the table top 110 and comprises a Y-axis beam, a liquid adding arm capable of moving on the Y-axis beam and a liquid adding module arranged on the liquid adding arm; the liquid adding module can add the reagent in the reagent bottle to the slide module of the supporting plate assembly through the liquid adding opening.

Optionally, the wheel-disk immunohistochemical apparatus further comprises:

a plurality of immersion tanks disposed on the work stage and capable of containing reagents to immerse the slide modules;

move piece mechanism, move piece mechanism and be located the top of workstation just move piece mechanism include the X axle roof beam, can the Z axle roof beam that moves on the X axle roof beam and set up in move the piece arm on the Z axle roof beam, it can shift to move the piece arm the rim plate transport mechanism on the slide module with soak the inslot the slide module.

Optionally, the slide moving arm includes a telescopic arm disposed on the Z-axis beam and capable of moving along the Z-axis beam, a rotating plate rotatably mounted on the telescopic arm, a rotating motor mounted on the telescopic arm and used for driving the rotating plate to rotate, and a mechanical gripper disposed on the rotating plate and capable of gripping the slide module, wherein a rotation axis of the rotating plate is parallel to the X-axis beam; the mechanical gripper comprises paired fork rods arranged at the opposite ends of the rotating plate and clamping blocks arranged at the free ends of the fork rods, and the clamping blocks can be clamped into clamping grooves of the slide module; the rotating plate is provided with a scanning module.

Optionally, the fork rod is an electric telescopic rod, and a plane where the fork rod is located is parallel to the extending direction of the X-axis beam.

Optionally, a plurality of partition ribs distributed at intervals are arranged on the inner wall of the soaking tank, and the slide module can be clamped between the adjacent partition ribs;

the number of the soaking grooves is multiple, and each soaking groove is provided with a liquid inlet pipe and a liquid discharge pipe.

Optionally, the liquid adding module comprises a mounting seat capable of moving on the liquid adding arm, a mounting rod with one end arranged on the mounting seat, and a plurality of liquid adding needles mounted on the mounting rod, wherein the mounting seat is capable of moving in a vertical direction; the reagent tank assemblies are distributed at intervals along the extension direction of the Y-axis beam, and the liquid adding needle can move along with the liquid adding arm to absorb liquid from the reagent bottle and add the liquid to the slide module of the supporting plate assembly;

and/or the refrigerating unit comprises a radiating fan, a cold-surface radiating fin, a semiconductor refrigerating fin and a hot-surface radiating fin which are sequentially superposed, wherein the radiating fan is close to the bottom of the reagent tank assembly.

Optionally, the housing and the gear assemblies are arranged in pairs, and two corresponding transmission gears in the two gear assemblies are connected through the supporting plate assembly; the two center fixed gears are fixedly connected through the center connecting rod.

Optionally, the pallet assembly comprises a bottom plate, side plates, a slide module and a slide cover plate;

the glass slide module is arranged on the bottom plate, a plurality of grooves for placing the glass slides are formed in the glass slide module, and the glass slide cover plate is arranged above the glass slide module; the two ends of the bottom plate are provided with the side plates, and the side plates are fixedly connected with the transmission gear.

Optionally, the supporting plate assembly further comprises a transmission shaft and a flip motor, the flip motor is installed on the side plates, and two ends of the slide cover plate are connected with an output shaft of the flip motor through the transmission shaft.

(III) advantageous effects

The invention has the beneficial effects that: the wheel disc transport mechanism is placed in the constant temperature and humidity box to can carry out the liquid feeding to all slide modules through the liquid feeding opening, thereby can be in the in-process of carrying out periodic transportation to layer board subassembly, accomplish simultaneously and carry out the liquid feeding to all slide modules (the liquid feeding action can carry out many times in a plurality of rotation periods, the reagent of liquid feeding at every turn also can be different) and incubate all slide modules. Compared with the existing mode that liquid adding and incubation are divided into two independent programs and the slide module needs to be repeatedly transferred between the liquid adding station and the incubation station, the method can greatly simplify the operation flow of immunohistochemistry, reduce the transfer operation steps, reduce the loss caused by improper operation in the transfer process, improve the treatment efficiency and increase the treatment flux. And moreover, the wheel disc transfer mechanism can reduce the space occupation of the upper table surface of the workbench, and the processing flux can be greatly improved on the premise of not increasing the volume of the whole machine.

The liquid feeding arm of the liquid feeding mechanism drives the liquid feeding module to move, so that liquid feeding is carried out on different slide modules, and pollution of reagents such as primary antibody during dripping can be reduced. The common cooperation of the wheel disc transfer mechanism and the liquid feeding arm can reduce manual operation to a greater extent, and the immunohistochemical process is more automatic, so that the machine flux can be greatly increased. In addition, set up reagent groove subassembly on the workstation, realize the low temperature storage to reagent, reagent need not take repeatedly, can promote experimental efficiency by a wide margin. By the technical scheme, the experimental flux can be greatly increased, and the automatic immunohistochemical experimental process can be optimized.

Drawings

FIG. 1 is a schematic structural view of a wheel-disk immunohistochemical apparatus according to the present invention;

FIG. 2 is a schematic structural view of the wheel disk immunohistochemical instrument in FIG. 1 after the box shell and the constant temperature and humidity box are removed;

FIG. 3 is a schematic structural view of the disk-type immunohistochemical apparatus of FIG. 1 in a case-removed state;

FIG. 4 is a schematic structural diagram of the wheel transfer mechanism in FIG. 2;

FIG. 5 is a side view of the wheel disc assembly of FIG. 4;

FIG. 6 is a front view of the wheel transfer mechanism of FIG. 4;

FIG. 7 is an enlarged view taken at A in FIG. 6;

fig. 8 is an enlarged view at B in fig. 6;

FIG. 9 is a schematic structural view of the pallet assembly of FIG. 4;

FIG. 10 is a schematic diagram of the reagent reservoir assembly of FIG. 2;

FIG. 11 is a front cross-sectional view of FIG. 10;

FIG. 12 is a schematic view of the liquid adding mechanism of FIG. 2;

FIG. 13 is a schematic view of the construction of the steeping cistern in FIG. 2;

FIG. 14 is an enlarged view at C of FIG. 13;

FIG. 15 is a schematic structural view of the sheet moving mechanism in FIG. 2;

fig. 16 is an enlarged view of fig. 15 at D.

[ instruction of reference ]

100: a work table; 110: a table top; 120: a column; 130: a foot plate; 140: a liquid feeding opening;

200: a wheel disc transfer mechanism; 1: a wheel disc assembly; 11: a housing; 12: a gear assembly; 121: a gear is fixed at the center; 122: a transition gear; 123: a transmission gear; 13: a central connecting rod; 14: a bearing; 2: a pallet assembly; 21: a base plate; 22: a side plate; 23: a slide module; 231: a card slot; 24: a glass slide; 25: a slide cover plate; 26: a drive shaft; 27: a flip motor; 3: a support; 31: a drive mechanism; 311: a wheel motor; 312: a drive plate; 313: a motor fixing plate;

400: a reagent reservoir assembly; 410: a reagent bottle; 420: a refrigeration unit; 421: a heat-dissipating fan; 422: a cold side heat sink; 423: a semiconductor refrigeration sheet; 424: a hot-side heat sink;

500: a liquid adding mechanism; 510: a Y-axis beam; 520: a liquid adding arm; 530: a liquid adding module; 531: a mounting seat; 532: mounting a rod; 533: a liquid adding needle;

600: a soaking tank; 610: separating the bars;

700: a sheet moving mechanism; 710: an X-axis beam; 720: a Z-axis beam; 730: a sheet moving arm; 731: a telescopic arm; 732: a rotating plate; 733: a rotating electric machine; 734: a mechanical gripper; 735: a fork lever; 736: a clamping block; 737: a scanning module;

800: a constant temperature and humidity chamber; 810: an electrical heating rod;

900: a box shell; 910: a box body; 920: and a box cover.

Detailed Description

For the purpose of better explaining the present invention and to facilitate understanding, the present invention will be described in detail by way of specific embodiments with reference to the accompanying drawings.

It should be noted that all directional indicators (such as up, down, left, right, front, and back \8230;) in the embodiments of the present invention are only used to explain the relative positional relationship between the components, the motion situation, etc. in a specific posture (as shown in the attached drawings), and if the specific posture is changed, the directional indicators are changed accordingly.

In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise explicitly stated or limited, the terms "connected", "fixed", and the like are to be understood broadly, for example, "fixed" may be fixedly connected, may be detachably connected, or may be integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

As shown in fig. 1 to 3, the present invention provides a wheel-disk immunohistochemical apparatus, which includes a work table 100, a wheel disk transport mechanism 200, a constant temperature and humidity chamber 800, a reagent tank assembly 400, and a liquid adding mechanism 500. With reference to fig. 4, the wheel disc transferring mechanism 200 includes a support 3 disposed in the work table 100, a wheel disc assembly 1 rotatably disposed on the support 3, and a plurality of pallet assemblies 2 for carrying the slide modules 23; a plurality of layer board subassembly 2 intervals set up on wheel disc subassembly 1 and can follow wheel disc subassembly 1 and rotate around the central axis of wheel disc subassembly 1, and at wheel disc subassembly 1's rotatory in-process, all layer board subassemblies 2 remain unchanged with the contained angle of horizontal plane. The constant temperature and humidity chamber 800 is arranged in the workbench 100 and below the table top 110 (see fig. 3), and the wheel disc transfer mechanism 200 is accommodated in the constant temperature and humidity chamber 800 (wherein the bracket 3 is fixedly arranged in the constant temperature and humidity chamber 800) to incubate the slide module 23 on the wheel disc transfer mechanism 200; the table top 110 of the workbench 100 is provided with a liquid feeding opening 140 corresponding to the top of the wheel disc transfer mechanism 200. The reagent tank assembly 400 is provided on the work table 100, and a plurality of reagent bottles 410 for storing reagents and a refrigerating unit 420 for refrigerating the reagent bottles 410 are provided in the reagent tank assembly 400. In addition, the liquid adding mechanism 500 is located above the table top 110 and the liquid adding mechanism 500 comprises a Y-axis beam 510, a liquid adding arm 520 capable of moving on the Y-axis beam 510, and a liquid adding module 530 arranged on the liquid adding arm 520; the priming module 530 is capable of adding reagent from the reagent bottles 410 through the priming opening 140 onto the slide module 23 of the pallet assembly 2.

The constant temperature and humidity chamber 800 has a main function of providing a relatively stable constant temperature and humidity environment after the liquid is added to the slide 24 on the slide module 23, so as to complete the incubation of the experimental object on the slide 24. A constant temperature component and a humidifier are arranged in the constant temperature and humidity box 800, and the constant temperature and humidity box 800 realizes the heat preservation function through the constant temperature component inside, so that the internal temperature is kept at about 37 ℃; the humidity control is performed by the humidifier to adjust the internal humidity within a suitable range. The constant temperature component can be a combination of an electric heating rod 810 and a fan or a combination of a semiconductor refrigeration piece and a fan, and the heat surface of the semiconductor refrigeration piece can conduct heat into the constant temperature and humidity box 800; or may be a cold and hot air conditioning system.

In the above embodiment, the wheel disk transfer mechanism 200 is placed in the constant temperature and humidity chamber 800 as a whole, and all the slide modules 23 can be filled with liquid through the liquid filling opening 140, so that in the process of carrying out periodic transfer on the pallet assembly 2, all the slide modules 23 can be filled with liquid (the liquid filling action can be carried out for a plurality of times in a plurality of rotation periods, and the reagent added with liquid at each time can be different) and all the slide modules 23 can be incubated. The liquid adding and incubation of the glass slide 24 are completed on the wheel disc transfer mechanism 200, namely the glass slide 24 can complete the incubation function in the wheel disc transfer mechanism 200, and the efficiency of the whole process can be improved. Compared with the existing mode that liquid adding and incubation are divided into two independent programs and the slide module 23 needs to be repeatedly transferred between the liquid adding station and the incubation station, the invention can greatly simplify the operation flow of immunohistochemistry, reduce the transfer operation steps, reduce the loss caused by improper operation in the transfer process, improve the treatment efficiency and increase the treatment flux. Moreover, the wheel disc transferring mechanism 200 is positioned below the table top 110, so that the space occupation of the table top 110 on the workbench 100 can be reduced, and the processing flux can be greatly improved on the premise of not increasing the volume of the whole machine by using a rotation mode.

The liquid adding arm 520 of the liquid adding mechanism 500 drives the liquid adding module 530 to move, so that liquid is added to different slide modules 23, and pollution of primary antibody reagents during dripping can be reduced. The joint cooperation of the wheel disc transferring mechanism 200 and the liquid adding arm 520 can reduce manual operation to a greater extent, so that the immunohistochemical process is more automated, and the machine flux can be greatly increased. In addition, set up reagent trough subassembly 400 on workstation 100 to realize the low temperature storage to the reagent, the reagent can directly be placed in the whole machine for a long time, need not take repeatedly, can promote experimental efficiency by a wide margin. By the technical scheme, the experimental flux can be greatly increased, and the automatic immunohistochemical experimental process can be optimized.

Referring to fig. 1 again, the wheel-disc immunohistochemistry apparatus may further include a case 900, and the case 900 may wrap the workbench 100 and each functional module disposed on the workbench 100, so as to create a clean environment for experimental operations. The housing 900 includes a housing 910 and a cover 920 hingedly attached to the housing 910, and opening the cover 920 facilitates proper operator intervention for immunohistochemical testing. In addition, referring to fig. 2, the table 100 includes a table top 110 and a foot plate 130 parallel to each other and a plurality of columns 120 connecting the table top 110 and the foot plate 130.

In a preferred embodiment, as shown in fig. 2 and 13 to 16, the carousel immunohistochemistry apparatus further includes a plurality of immersion tanks 600 and a sheet moving mechanism 700, and the immersion tanks 600 are disposed on the work stage 100 and can accommodate reagents to immerse the slide modules 23. The immersion tank 600 can provide reaction environments required for experimental procedures, such as: dewaxing, dewatering, cleaning, etc. Each soaking tank 600 is connected with a liquid inlet pipe and a liquid outlet pipe, and the functions of adding different reagents into the soaking tank 600 and discharging waste liquid in the soaking tank 600 can be realized through pipeline parts configured by the whole machine. In addition, in the preferred embodiment, a plurality of immersion tanks 600 (six in fig. 2) are arranged in the wheel-disk immunohistochemical apparatus, and any one of the immersion tanks 600 can be used as a slide rack for temporarily placing the slide module 23, and the liquid in the immersion tank 600 as the slide rack needs to be drained, so that a plurality of experimental procedures can be performed simultaneously, and the throughput of the whole apparatus can be further improved. Be provided with the subfield muscle 610 of a plurality of interval distributions on soaking groove 600's the inner wall, the subfield muscle 610 all extends along upper and lower direction slope, and slide module 23 can block locates between the adjacent subfield muscle 610 to can place a plurality of slide modules 23 side by side in a soaking groove 600, can place polylith slide 24 (see fig. 13) side by side on every slide module 23, can not influence each other between the adjacent slide module 23, thereby can promote the flux of complete machine by a wide margin.

As shown in fig. 2 and 15, the slide moving mechanism 700 is located above the worktable 100 and the slide moving mechanism 700 includes an X-axis beam 710, a Z-axis beam 720 capable of moving on the X-axis beam 710, and a slide moving arm 730 disposed on the Z-axis beam 720, so that the slide moving arm 730 can move in the X direction and the Z direction to transfer the slide module 23 on the wheel disc transfer mechanism 200 and the slide module 23 in the immersion tank 600, and the automation operation is realized in each flow of the immunohistochemistry experiment, thereby reducing the burden of the operator and improving the processing efficiency of the whole machine. In other embodiments, the slide moving mechanism 700 may have other forms as long as the action of grasping and transporting the slide module 23 is achieved.

It should be noted that, every two of the X-axis beam 710, the Y-axis beam 510, and the Z-axis beam 720 are perpendicular to each other, the X-axis beam 710 corresponds to the X-axis direction, the Y-axis beam 510 corresponds to the Y-axis direction, and the Z-axis beam 720 corresponds to the Z-axis direction, so that a three-axis coordinate system can be formed to facilitate positioning during experimental operation. In the embodiment shown in fig. 2 and 3, the X-axis beam 710 and the Y-axis beam 510 are disposed in a horizontal direction, the Z-axis beam 720 is disposed in a vertical direction, and all of the three are located above the worktable 100 so as to operate each function module disposed on the worktable 100. In addition, the Z-axis beam 720 may be driven by a combination of motors and transmissions (e.g., pulleys), pneumatic or hydraulic cylinders, etc. to reciprocate along the X-axis beam 710, or other known driving means may be used to achieve directional movement of the Z-axis beam 720. The driving method for moving the sheet transfer arm 730 on the Z-axis beam 720 and the driving method for moving the liquid adding arm 520 on the Y-axis beam 510 can be set with reference to the method for moving the Z-axis beam 720 on the X-axis beam 710.

In a preferred embodiment, referring again to fig. 15, the slide moving arm 730 includes a telescopic arm 731 which is provided on the Z-axis beam 720 and can move along the Z-axis beam 720, a rotating plate 732 which is rotatably mounted on the telescopic arm 731, a rotating motor 733 which is mounted on the telescopic arm 731 and drives the rotating plate 732 to rotate (an output shaft of the rotating motor 733 can transmit torque to the rotating plate 732 by a gear transmission manner), and a mechanical gripper 734 which is provided on the rotating plate 732 and can grip the slide module 23, wherein a rotation axis of the rotating plate 732 is parallel to an extending direction of the X-axis beam 710; the mechanical gripper 734 includes a pair of fork bars 735 mounted on opposite ends of the swivel plate 732 and a latch 736 disposed on a free end of the fork bars 735, the latch 736 being capable of latching into a latch slot 231 (see fig. 14) of the slide module 23; the rotating plate 732 is provided with a scanning module 737. The telescopic arm 731 can be extended and retracted in the Y-axis direction, and the rotating plate 732 can be rotated based on the rotation axis, so that the fork bar 735 on the rotating plate 732 can integrally realize spatial movement in six directions and circumferential rotation of 0-90 degrees around the X-axis direction, that is, the change in the angle of the slide module 23 (from vertical to horizontal or from horizontal to vertical) can be realized after the mechanical gripper 734 grasps the slide module 23, so as to meet the transportation requirement of the slide module 23. The slide moving mechanism 700 grabs, rotates and moves the slide module 23, so that the slide module 23 can move in the height direction and among the functional areas; the scanning module 737 may be specifically a camera or other scanner, which can scan and identify the information of the slide module 23 to realize the precise positioning and tracking of each slide module 23, ensure the accuracy of the immunohistochemical experiment, and make the immunohistochemical experiment process traceable.

Moreover, as shown in fig. 16, the fixture block 736 at the free end of the fork bar 735 can be matched with the slot 231 of the slide module 23, the protrusions disposed at two opposite sides of the fixture block 736 can prevent the fixture block 736 from rotating in the slot 231, the slots 231 of the slide module 23 are disposed in pairs, the fork bar 735 is also disposed in pairs, the slots 231 are plugged with the fixture block 736 in a one-to-one correspondence manner, so as to ensure the reliability of grasping the slide module 23, and prevent the slide module 23 from falling off and causing loss during transportation. The grabbing structure is simple, the occupied space is small, and the grabbing device is also suitable for a vertical grabbing mode of plates. In other embodiments, the mechanical grip 734 may take other forms, including, for example, two hinged jaws that open and close to each other to grasp the slide module 23.

The fork bar 735 is an electric telescopic bar or an electric push rod, and a plane where the fork bar 735 is located is parallel to the extending direction of the X-axis beam 710. When fork arm 735 is pegged graft with draw-in groove 231, fork arm 735 can stretch out and draw back according to the demand, guarantees going on smoothly of the process of pegging graft. Specifically, when the fork bar 735 is aligned with the slot 231, if there is only a small difference, the insertion can be completed directly by extending the fork bar 735, without readjusting the state of the entire sheet moving mechanism 700, so that the control requirement for the entire sheet moving mechanism 700 can be simplified.

Further, as shown in fig. 12, the charging module 530 includes a mounting base 531 capable of moving on the charging arm 520, a mounting rod 532 having one end disposed on the mounting base 531, and a plurality of charging needles 533 mounted on the mounting rod 532, wherein the mounting base 531 moves in the vertical direction (Z-axis direction); the reagent tank assemblies 400 are spaced along the extension direction of the Y-axis beam 510, and the filling needles 533 can move along with the filling arm 520 to suck liquid from the reagent bottles 410 and add the liquid to the slide module 23 of the pallet assembly 2. The filling needle 533 can be connected to a corresponding plunger pump (not shown) for squeeze filling. Specifically, the pump head end of the plunger pump is provided with an inlet and an outlet, when the plunger pump works, liquid enters the cavity inside the plunger pump through the inlet and is then discharged through the outlet, and the liquid adding mode is realized by drawing and pulling the push rod in the cavity of the plunger pump to suck and discharge the liquid out of the cavity. The plunger pump can be connected with the upper end of liquid feeding needle 533 through the pipeline respectively to rely on the plunger pump extrusion, come liquid to push the pipeline, get into liquid feeding needle 533 through the pipeline again, realize the dropwise add of liquid, and each primary antibody reagent adopts independent pipeline, need not to wash the needle, also does not have the cross risk. Furthermore, referring to fig. 2, the reagent tank assemblies 400 are distributed at intervals along the extending direction of the Y-axis beam 510, and the liquid adding opening 140 is disposed between the two reagent tank assemblies 400, so that the liquid adding needle 533 can move between the reagent tank assemblies 400 and the wheel disk transportation mechanism 200 to perform liquid sucking and adding actions.

In addition, as shown in fig. 10 and 11, the refrigeration unit 420 includes a heat dissipation fan 421, a cold-side heat dissipation plate 422, a semiconductor refrigeration plate 423, and a hot-side heat dissipation plate 424, which are stacked in this order, and the heat dissipation fan 421 is disposed near the bottom of the reagent tank assembly 400. The refrigeration unit 420 includes a plurality of semiconductor refrigeration sheets 423 distributed in an array, and the semiconductor refrigeration sheets 423 are all used for heating at one side and refrigerating at the other side, in fig. 11, a cold-side heat sink 422 is arranged above the semiconductor refrigeration sheets 423, and a hot-side heat sink 424 is arranged below the semiconductor refrigeration sheets 423, that is, the heat dissipation fan 421 makes cold air generated by the cold side of the semiconductor refrigeration sheets 423 blow cold air into the reagent tank assembly 400 through a through hole at the bottom of the reagent tank assembly 400, and the hot-side heat sink 424 can quickly remove heat generated by the hot side of the semiconductor refrigeration sheets 423. Under the action of the cold-side radiating fins 422 and the semiconductor refrigerating fins 423, the reagent tank assembly 400 can always keep the temperature at about 4 ℃, and the reagent bottles 410 which keep the temperature low can respectively contain various reagents for experiments. Wherein each reagent well assembly 400 may contain a plurality of reagent bottles 410 therein, and an anti-reagent can be stored in the reagent bottles 410.

Alternatively, in other embodiments, refrigeration unit 420 may also be an evaporator of a refrigeration system or the like, so long as low temperatures are maintained within reagent tank assembly 400. Set up the reagent cell subassembly 400 that can carry out cryogenic storage on workstation 100 to realize the long-term low temperature storage to reagent, reagent need not take from the outside repeatedly, thereby can promote experimental efficiency by a wide margin. Moreover, a plurality of reagent bottles 410 in reagent trough subassembly 400 can be respectively the one-to-one corresponding to a plurality of liquid feeding needles 533, and liquid feeding needle 533 can directly drip the reagent of saving in reagent bottle 410 on slide glass 24 through the plunger pump to form linkage operation, thereby make reagent such as anti can swiftly and smoothly drip, in order to shorten experiment operating time and increase experiment flux.

Further, as shown in fig. 4 to 8, the wheel assembly 1 includes a housing 11 rotatably mounted on the stand 3, a gear assembly 12 and a center connection rod 13 both mounted on the housing 11, the gear assembly 12 including a plurality of gear sets. As shown in fig. 5, the gear set includes a center fixed gear 121, a plurality of transition gears 122, and a transmission gear 123 arranged in sequence, and adjacent gears in the gear set are engaged with each other; the center fixed gear 121, the transition gear 122 and the transmission gear 123 are rotationally connected with the shell 11, the center fixed gear 121 is fixedly connected with the center connecting rod 13, and the center connecting rod 13 is fixed on the bracket 3; each gear set on the same housing 11 shares a central fixed gear 121. A plurality of gear sets are arranged on the shell 11, adjacent gears in the gear sets are meshed to form a planetary gear train, and then the supporting plate assembly 2 used for placing the glass slide 24 is fixedly connected to the transmission gear 123 on the outermost circle, so that the space utilization rate is improved, and the flux of the immunohistochemical instrument is greatly improved. The joint of the supporting plate component 2 and the transmission gear 123 is located at the center of the end face of the transmission gear 123, so that the track of the supporting plate component 2 is circular when the supporting plate component moves along with the revolution of the transmission gear 123, and the slide module 23 is convenient to take out and place.

The bracket 3 is provided with a driving mechanism 31 for driving the shell 11 to rotate; the pallet assembly 2 is fixedly connected with the transmission gear 123, and the included angle between all the pallet assemblies 2 and the horizontal plane is kept unchanged in the rotation process of the shell 11. As shown in fig. 6 to 8, the driving mechanism 31 may include a wheel motor 311, a motor fixing plate 313 and a driving plate 312, wherein the wheel motor 311 is fixedly mounted on the bracket 3 through the motor fixing plate 313, the driving plate 312 is fixedly connected with the housing 11, and the driving plate 312 is connected with an output shaft of the wheel motor 311. When the wheel motor 311 drives the housing 11 to rotate through the driving plate 312, the included angle between the slide 24 placed in the tray assembly 2 and the horizontal plane is not changed, and in the preferred embodiment, the included angle between the slide 24 and the horizontal plane is 0-20 degrees, which facilitates the flow of liquid on the slide 24.

Referring to fig. 4 to 6, the housing 11 and the gear assemblies 12 are both arranged in pairs, and two corresponding transmission gears 123 in the two gear assemblies 12 are connected through the supporting plate assembly 2, that is, two ends of the supporting plate assembly 2 are respectively and fixedly connected with the two corresponding transmission gears 123 on the pair of housings 11 in a one-to-one correspondence manner, so that the supporting plate assembly 2 is more firmly installed, and in the process of moving along with the transmission gears 123, the falling-off condition cannot occur, and the paired housings 11 are ensured to keep synchronous movement. The two center fixed gears 121 are fixedly connected by the center connecting rod 13, so that the two center fixed gears 121 are ensured to be stationary at the same time, and further, the movement states of the corresponding pairs of transmission gears 123 at the outermost periphery are ensured to be the same. Further, in order to maintain the synchronized rotation of the paired housings 11, the two housings 11 may be integrated by fixedly mounting a synchronizing lever at the outermost ends of the two housings 11; the two shells 11 can be driven to rotate by belt transmission, and a belt for driving the two shells 11 to rotate is connected to the same wheel motor 311, as long as the effect of synchronous rotation of the two shells 11 can be achieved.

Further, as shown in fig. 9, the blade assembly 2 includes a bottom plate 21, side plates 22, a slide module 23, and a slide cover plate 25. The slide module 23 is arranged on the bottom plate 21, a plurality of grooves for placing the slide 24 are arranged on the slide module 23, and the slide cover plate 25 is arranged above the slide module 23; the two ends of the bottom plate 21 are provided with side plates 22, and the side plates 22 are fixedly connected with the transmission gear 123, so as to facilitate the installation of the pallet assembly 2. The supporting plate component 2 further comprises a transmission shaft 26 and a flip motor 27, the side plates 22 are all provided with the flip motor 27, two ends of the slide cover plate 25 are connected with an output shaft of the flip motor 27 through the transmission shaft 26, the flip motor 27 drives the slide cover plate 25 to rotate, and then the slide cover plate 25 can press the slide module 23, and further the slide 24 can be pressed according to requirements. When needing to take out slide module 23 from bottom plate 21 or when placing slide module 23 on bottom plate 21, only need control slide apron 25 rotation to open to place slide module 23 the corresponding position on bottom plate 21 can, in the same way after slide module 23 is placed on bottom plate 21, only need control slide apron 25 rotation to cover in the top of slide module 23, make the recess on slide module 23 become mutually independent cavity, remove liquid for subsequent liquid feeding and provide operating condition.

An odd number of transition gears 122 are included in any one gear set. On the same housing 11, the gear set is disposed on the housing 11 in an annular array with the central fixed gear 121 as the center, the number of the transition gears 122 is set to be odd, so that the rotation directions of the transmission gear 123 and the housing 11 are opposite, that is, the housing 11 can drive the transmission gear 123 to revolve around the rotation center of the housing 11 when rotating, and the transmission gear 123 can also rotate around the connection part of the transmission gear 123 and the housing 11 (the directions of revolution and rotation are opposite), and meanwhile, the number of teeth of the transmission gear 123 is equal to that of the central fixed gear 121, so that the rotation of the transmission gear 123 can offset the variation of the angle of the revolution driving itself, and the included angle between the bottom plate 21 in the pallet assembly 2 mounted on the transmission gear 123 and the horizontal plane can not be changed, and further, the included angle between the slide glass 24 on the bottom plate 21 and the horizontal plane can not be changed. Meanwhile, the included angle between the bottom plate 21 and the horizontal plane is only related to the installation included angle between the bottom plate 21 and the side plate 22, so that conditions are provided for placing the slide module 23 on the bottom plate 21 and preventing the slide module 23 from falling or falling off in the rotating process. In a more preferred embodiment, the included angle between the bottom plate 21 and the horizontal plane is 0 to 20 degrees, and after the bottom plate 21 has a certain included angle with the horizontal plane, the included angle between the slide glass 24 on the slide module 23 and the horizontal plane is 0 to 20 degrees, so that liquid after subsequent liquid adding can conveniently flow on the slide glass 24.

Further, there are three transition gears 122 in any one gear set, and the center fixed gear 121 is connected to the housing 11 through the bearing 14. The space utilization can be maximally improved by installing three transition gears 122 in the same gear set, and the housing 11 and the center fixed gear 121 are connected by the bearing 14, so that the fixing of the center fixed gear 121 does not affect the rotation of the housing 11.

The technical solution of the present invention will be further explained by describing the operation process of the roulette type immunohistochemical apparatus in detail. The working process of the wheel disk type immunohistochemical instrument comprises the following steps:

1. placing the glass slide 24 into the glass slide module 23, and placing the glass slide module 23 into a glass slide frame after the placing is finished;

2. the slide moving arm 730 moves along the X-axis beam 710 and the Z-axis beam 720, scans codes through the camera to identify the slide 24, sends identification information to an upper computer, and moves the slide module 23 to the soaking tank 600 through the mechanical gripper 734 after the identification is finished; in the plurality of soaking tanks 600, the functions of dewaxing, dewatering, cleaning and the like can be respectively realized, and the slide moving arm 730 can move the slide module 23 to each soaking tank 600 for corresponding functions;

3. after the process of the soaking tank 600 is completed, the slide module 23 is moved to the pallet assembly 2 of the wheel disc transfer mechanism 200 by the slide moving arm 730; the liquid adding arm 520 adds liquid to the slide 24;

4. after adding liquid, the electric heating rod 810 starts incubation when working, and at the moment, the glass slide 24 on the wheel disc transport mechanism 200 in the constant temperature and humidity box 800 is in a constant temperature and humidity environment until incubation is finished;

5. after the incubation is completed, the slide moving arm 730 moves the slide module 23 to the soaking tank 600 for soaking with the cleaning solution;

6. after cleaning, the slide module 23 is moved to the slide rack by the slide moving arm 730 and then air-dried for the next procedure.

It should be understood that the above description of the specific embodiments of the present invention is provided for illustration only, and the purpose of the present invention is to enable those skilled in the art to understand the content of the present invention and to implement the present invention, but the present invention is not limited to the specific embodiments described above. It is intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.

Claims

1. A wheel disk immunohistochemical appearance, characterized in that, wheel disk immunohistochemical appearance includes: a work table;

the gear set comprises a center fixed gear, a plurality of transition gears and a transmission gear which are sequentially arranged, and adjacent gears in the gear set are meshed with each other; the central fixed gear, the transition gear and the transmission gear are rotationally connected with the shell, the central fixed gear is fixedly connected with the central connecting rod, and the central connecting rod is fixed on the bracket; each of said gear sets on the same said housing sharing one of said center fixed gears;

the bracket is provided with a driving mechanism for driving the shell to rotate; the supporting plate components are fixedly connected with the transmission gear, and in the rotation process of the shell, included angles between all the supporting plate components and the horizontal plane are kept unchanged;

the constant temperature and humidity box is arranged in the workbench, and the wheel disc transport mechanism is accommodated in the constant temperature and humidity box so as to incubate the slide module on the wheel disc transport mechanism; a liquid adding opening corresponding to the top of the wheel disc transferring mechanism is formed in the table top of the workbench;

the liquid adding mechanism is positioned above the table board and comprises a Y-axis beam, a liquid adding arm capable of moving on the Y-axis beam and a liquid adding module arranged on the liquid adding arm; the liquid adding module can add the reagent in the reagent bottle to the slide module of the supporting plate assembly through the liquid adding opening.

2. The wheel-disk immunohistochemical apparatus according to claim 1, wherein: the wheel disk type immunohistochemical instrument further comprises:

move a mechanism, it is located to move a mechanism the top of workstation just move a mechanism and include the X axle beam, can the Z axle beam that moves on the X axle beam and set up in move a arm on the Z axle beam, it can shift to move a arm the rim plate transport mechanism on the slide module with soak the inslot the slide module.

3. The wheel-disk immunohistochemical apparatus according to claim 2, wherein: the slide moving arm comprises a telescopic arm which is arranged on the Z-axis beam and can move along the Z-axis beam, a rotating plate which is rotatably arranged on the telescopic arm, a rotating motor which is arranged on the telescopic arm and is used for driving the rotating plate to rotate, and a mechanical gripper which is arranged on the rotating plate and can grab the slide module, wherein the rotating axis of the rotating plate is parallel to the X-axis beam; the mechanical gripper comprises paired fork rods arranged at the opposite ends of the rotating plate and clamping blocks arranged at the free ends of the fork rods, and the clamping blocks can be clamped into clamping grooves of the slide module; the rotating plate is provided with a scanning module.

4. The wheel-disk immunohistochemical apparatus according to claim 3, wherein: the fork rod is an electric telescopic rod, and the plane where the fork rod is located is parallel to the extending direction of the X-axis beam.

5. The wheel-disk immunohistochemical apparatus according to claim 2, wherein: a plurality of partition ribs distributed at intervals are arranged on the inner wall of the soaking groove, and the glass slide module can be clamped between the adjacent partition ribs;

6. The wheel-disk immunohistochemical apparatus according to any one of claims 1 to 5, wherein: the liquid adding module comprises a mounting seat capable of moving on the liquid adding arm, a mounting rod with one end arranged on the mounting seat, and a plurality of liquid adding needles mounted on the mounting rod, wherein the mounting seat can move along the vertical direction; the reagent tank assemblies are distributed at intervals along the extension direction of the Y-axis beam, and the liquid adding needle can move along with the liquid adding arm to absorb liquid from the reagent bottle and add the liquid to the slide module of the supporting plate assembly;

and/or the refrigerating unit comprises a heat radiation fan, a cold surface heat radiation fin, a semiconductor refrigeration fin and a hot surface heat radiation fin which are sequentially overlapped, wherein the heat radiation fan is arranged close to the bottom of the reagent tank assembly.

7. The wheel disk immunohistochemical apparatus according to any one of claims 1 to 5, wherein: the shell and the gear assemblies are arranged in pairs, and two corresponding transmission gears in the two gear assemblies are connected through the supporting plate assembly; the two center fixed gears are fixedly connected through the center connecting rod.

8. The wheel-disk immunohistochemical apparatus according to claim 7, wherein: the supporting plate assembly comprises a bottom plate, a side plate, a slide module and a slide cover plate;

9. The wheel-disk immunohistochemical apparatus according to claim 8, wherein: the supporting plate component further comprises a transmission shaft and a flip motor, the flip motor is mounted on the side plate, and two ends of the slide cover plate are connected with an output shaft of the flip motor through the transmission shaft.