CN111398226A - CdTe quantum dot multi-channel fluorescence sensor based on ultraviolet irradiation and application method - Google Patents
CdTe quantum dot multi-channel fluorescence sensor based on ultraviolet irradiation and application method Download PDFInfo
- Publication number
- CN111398226A CN111398226A CN202010151907.3A CN202010151907A CN111398226A CN 111398226 A CN111398226 A CN 111398226A CN 202010151907 A CN202010151907 A CN 202010151907A CN 111398226 A CN111398226 A CN 111398226A
- Authority
- CN
- China
- Prior art keywords
- cdte quantum
- fluorescence
- biochemical substances
- quantum dot
- ultraviolet irradiation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229910004613 CdTe Inorganic materials 0.000 title claims abstract description 41
- 239000002096 quantum dot Substances 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 22
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 19
- 235000000346 sugar Nutrition 0.000 claims description 26
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 claims description 20
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 20
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 20
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 20
- 239000000126 substance Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims description 11
- RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 claims description 10
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims description 10
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- SRBFZHDQGSBBOR-KLVWXMOXSA-N beta-L-arabinopyranose Chemical compound O[C@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-KLVWXMOXSA-N 0.000 claims description 10
- 235000010355 mannitol Nutrition 0.000 claims description 10
- 230000008859 change Effects 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 6
- 230000001678 irradiating effect Effects 0.000 claims description 4
- 229910001385 heavy metal Inorganic materials 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 9
- 238000001514 detection method Methods 0.000 abstract description 9
- 238000003786 synthesis reaction Methods 0.000 abstract description 9
- 238000010438 heat treatment Methods 0.000 abstract description 6
- 238000002360 preparation method Methods 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 abstract description 3
- 239000003054 catalyst Substances 0.000 abstract description 2
- 230000004907 flux Effects 0.000 abstract description 2
- 239000003446 ligand Substances 0.000 abstract description 2
- YKYOUMDCQGMQQO-UHFFFAOYSA-L cadmium dichloride Chemical compound Cl[Cd]Cl YKYOUMDCQGMQQO-UHFFFAOYSA-L 0.000 description 23
- 239000000243 solution Substances 0.000 description 22
- CWERGRDVMFNCDR-UHFFFAOYSA-N thioglycolic acid Chemical compound OC(=O)CS CWERGRDVMFNCDR-UHFFFAOYSA-N 0.000 description 20
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 150000008163 sugars Chemical class 0.000 description 10
- 229910021642 ultra pure water Inorganic materials 0.000 description 8
- 239000012498 ultrapure water Substances 0.000 description 8
- 239000011259 mixed solution Substances 0.000 description 7
- PORWMNRCUJJQNO-UHFFFAOYSA-N tellurium atom Chemical compound [Te] PORWMNRCUJJQNO-UHFFFAOYSA-N 0.000 description 7
- 238000000513 principal component analysis Methods 0.000 description 6
- 229910000033 sodium borohydride Inorganic materials 0.000 description 6
- 239000012279 sodium borohydride Substances 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 239000011261 inert gas Substances 0.000 description 4
- 238000003491 array Methods 0.000 description 3
- -1 cadmium chloride-thioglycolic acid Chemical compound 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- XONPDZSGENTBNJ-UHFFFAOYSA-N molecular hydrogen;sodium Chemical compound [Na].[H][H] XONPDZSGENTBNJ-UHFFFAOYSA-N 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 229910000059 tellane Inorganic materials 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000218378 Magnolia Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000005859 cell recognition Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 150000004678 hydrides Chemical class 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000008932 jinhong Substances 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 229910052714 tellurium Inorganic materials 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a multi-channel fluorescent sensor for identifying saccharides by CdTe quantum dots, a preparation method and an application method thereof. The synthesis of a plurality of sensor units modified by different ligands is not needed, the synthesis route of the double-color CdTe quantum dots is the same, and only the heating time is different; the analysis efficiency and the detection flux of the saccharides can be improved; a sensor array based on ultraviolet light irradiation is constructed, the ultraviolet light is used as a catalyst, the resolution ratio of the sensor array is greatly improved, and the method can be widened to the detection of other analytes.
Description
Technical Field
The invention relates to the field of nano sensors, in particular to the synchronous identification of multiple saccharides by an ultraviolet light guided multi-channel fluorescence sensor array.
Background
Saccharides are main energy substances of organisms, and the abnormal intake and metabolism of saccharides usually cause disorder of biological functions, and saccharides are important in various basic activities of life (such as proliferation and inflammation of cancer cells) as cell recognition information factors, and the change of saccharide content in the organisms has important indication significance for the generation of many diseases, so that the recognition and detection of various saccharides are realized, and the method has important roles in the fields of life science and the like. At present, sugar detection methods mainly comprise mass spectrometry, chromatography, electrochemical methods and the like, but the methods usually need complex pretreatment, expensive instruments or professional technicians and other necessary conditions, so that the application of the methods is limited, and the development of an effective method for rapidly and synchronously identifying multiple sugars at low cost has important significance.
The fluorescent sensor array is used for artificially simulating the olfactory system of the mammal, and can realize the identification of different substances according to fingerprint spectrums formed by response of a plurality of sensing units to fluorescent signals of different degrees of samples, and each target object can generate a unique fingerprint spectrum on the array, thereby realizing the distinguishing identification among the target objects; the sensor array abandons the traditional high-specificity key-lock combination mode, reduces the requirement on a specificity sensing unit, greatly saves the detection cost and time and can detect complex samples. In recent years, fluorescent sensor arrays have been widely used for identification and detection of sugars, proteins, serum, cells, and the like. The improvement of the resolution of the fluorescence sensor array mainly depends on increasing the number of sensing units, but the method involves the synthesis of a plurality of different fluorescent materials, which greatly increases the workload of researchers and makes the array very large. In contrast, if the signal channel of each sensing unit is increased or sensing units with different properties are synthesized by using the same process, the construction of the sensor array can be simplified, and the practicability and miniaturization of the sensor array are promoted. In recent years, a variety of nanomaterials or organic compounds have been developed by researchers for use in the construction of multi-channel sensor arrays, but in general, materials with multi-dimensional sensing mechanisms are still very rare, and often involve complex synthesis processes, and the need to use different instruments for detecting each channel signal limits the development of multi-channel sensor arrays.
Disclosure of Invention
The purpose of the invention is as follows: the invention provides a CdTe quantum dot multi-channel fluorescent sensor based on ultraviolet irradiation and an application and application method thereof, and solves the problems that the improvement of the resolution of the sensor depends on increasing the number of sensing units and greatly increasing the workload of researchers; meanwhile, the problems that materials of a multi-dimensional sensing mechanism are very rare, the synthesis process is complex, and different instruments are required to be used for detecting signals of each channel are solved.
The technical scheme is as follows: according to the CdTe quantum dot multichannel fluorescence sensor based on ultraviolet irradiation, the multichannel fluorescence sensor can emit yellow fluorescence or green fluorescence under the condition of ultraviolet irradiation.
The preparation method of the multi-channel fluorescent sensor for identifying the saccharides by the CdTe quantum dots comprises the following steps:
(1) under the protection of inert gas, tellurium powder and sodium borohydride (NaBH)4) Adding into pure water, mixing and reacting until the solution becomes clear and light pink, and obtaining sodium hydrogen telluride (NaHTe) solution.
(2) Cadmium chloride (CdCl)2·2.5H2O) and thioglycolic acid (TGA) are added into pure water to be mixed evenly, and the pH is adjusted to 9.0-9.4. Introducing inert gas to remove air, reacting and obtaining cadmium chloride-thioglycollic acid (CdCl)2-TGA) solution; among them, the pH is preferably 9.2.
(3) Adding the solution of sodium hydrogen telluride (NaHTe) obtained in the step (1) into the cadmium chloride-thioglycolic acid (CdCl) obtained in the step (2) under the protection of inert gas2-TGA) solution and mixed well to obtain an orange mixed solution.
(4) Dividing the mixed solution into a plurality of parts, respectively heating in an oven at 80-90 ℃ for 85-95min and 100-110min, and respectively obtaining green fluorescent CdTe quantum dots and yellow fluorescent CdTe quantum dots after cooling; wherein, the temperature is preferably 80 ℃, and the heating time is preferably 90min and 105min respectively.
Wherein in the step (1), the mass ratio of the tellurium powder to the sodium borohydride is 1.2:1-1.4: 1. Preferably 1.276: 1.
the concentrations of the tellurium powder and the sodium borohydride in pure water are 0.010-0.013g/ml and 0.08-0.12g/ml respectively. The tellurium powder and sodium borohydride preferably have concentrations of 0.01276g/ml and 0.01 g/ml.
Wherein in the step (2), the molar ratio of the cadmium chloride to the thioglycolic acid is 0.15:1-0.18: 1. Preferably 0.167: 1.
The concentration of the cadmium chloride and the thioglycolic acid in pure water is 0.0020 to 0.0025g/ml and 2.0 to 2.2 mu L/ml respectively, and the concentration of the cadmium chloride and the concentration of the thioglycolic acid are preferably 0.0023g/ml and 2.1 mu L/ml.
The pH was adjusted with sodium hydroxide (NaOH) particles.
In the above method step, the inert gas is high-purity nitrogen.
The CdTe quantum dot multichannel fluorescence sensor based on ultraviolet irradiation is applied to the identification of biochemical substances, the fluorescence color and the fluorescence intensity of the multichannel fluorescence sensor change under the continuous long-time irradiation of ultraviolet light, and different biochemical substances can be combined with the multichannel fluorescence sensor to generate interference of different degrees on the change of the fluorescence color and the fluorescence intensity of the multichannel fluorescence sensor, so that the different biochemical substances can be distinguished and identified.
The biochemical substances independently include saccharides, proteins and heavy metals.
The saccharide comprises one or more of D-mannitol, D- (+) -xylose, L- (+) -arabinose, D-mannose, D- (+) galactose, D-fructose and D-glucose.
The application method of the CdTe quantum dot multichannel fluorescence sensor based on ultraviolet irradiation for identifying biochemical substances comprises the following steps:
(1) dissolving different biochemical substances in pure water respectively to prepare solutions;
(2) mixing yellow and green fluorescent CdTe quantum dots with the solutions prepared in step (1), respectively, irradiating with 312 + -10 nm ultraviolet lamp for 20-30min, observing and recording the fluorescent color and intensity at the same wavelength, using secondary water instead of sugar solution as blank control,
(3) the aim of distinguishing and identifying different biochemical substances is achieved by observing the interference of different biochemical substances on the fluorescence color and the fluorescence intensity to different degrees.
The wavelength of ultraviolet light emitted by the ultraviolet lamp is 312 +/-10 nm.
The ultraviolet irradiation time is 20-30 min.
The carbohydrate comprises one or more of D-mannitol, D- (+) -xylose, L- (+) -arabinose, D-mannose, D- (+) galactose, D-fructose and D-glucose.
The method for identifying the saccharides by the CdTe quantum dot multichannel fluorescent sensor comprises the following steps:
(1) taking different kinds of sugar, respectively dissolving in pure water to obtain sugar solution with concentration of 1.8-2.2mg/ml, wherein the concentration of sugar solution is preferably 2.0mg/m L, and storing at 4 deg.C for use.
(2) Mixing yellow and green fluorescent CdTe quantum dots with the sugar solutions in step (1), respectively, irradiating with a 312 + -10 nm ultraviolet lamp for 20-30min, observing and recording the fluorescent color and intensity at the same wavelength, and using secondary water instead of sugar solution as blank control.
(3) The fluorescence signals were fingerprinted using Origin software and subjected to Principal Component Analysis (PCA) using SPSS software to further differentiate the sugars.
The sugar comprises one or more of D-mannitol, D- (+) -xylose, L- (+) -arabinose, D-mannose, D- (+) galactose, D-fructose and D-glucose.
In the preparation method and the application method, the pure water is ultrapure water.
Has the advantages that: 1. the invention uses the double-color CdTe quantum dots as the sensing units to construct a four-channel sensor array based on three primary colors (RGB) of fluorescence colors and fluorescence intensity (I), thereby realizing the rapid identification and detection of 7 kinds of sugar without expensive instruments or professional operators; 2. the synthesis of a plurality of sensor units modified by different ligands is not needed, the synthesis route of the double-color CdTe quantum dots is the same, and only the heating time is different; 3. the analysis efficiency and the detection flux of the saccharides can be improved; 4. a sensor array based on ultraviolet light irradiation is constructed, the ultraviolet light is used as a catalyst, the resolution ratio of the sensor array is greatly improved, and the method can be widened to the detection of other analytes.
Drawings
FIG. 1 is a graph showing the change of fluorescence color signals of 7 kinds of saccharides mixed with yellow and green fluorescent CdTe quantum dots, respectively, under different times of irradiation of ultraviolet light in example 2 of the present invention, wherein the upper column is a yellow fluorescent CdTe quantum dot sensor and the lower column is a green fluorescent CdTe quantum dot sensor, wherein a is D-mannitol, b is D- (+) -xylose, c is L- (+) -arabinose, D is D-mannose, e is D- (+) galactose, f is D-fructose, g is D-glucose, and h is a blank control;
FIG. 2 is a graph showing the change of fluorescence intensity signals of 7 kinds of saccharides mixed with yellow and green fluorescent CdTe quantum dots, respectively, under different times of irradiation of UV light in example 2 of the present invention, wherein the upper column is a yellow fluorescent CdTe quantum dot sensor and the lower column is a green fluorescent CdTe quantum dot sensor, wherein a is D-mannitol, b is D- (+) -xylose, c is L- (+) -arabinose, D is D-mannose, e is D- (+) galactose, f is D-fructose, g is D-glucose, and h is a blank control;
FIG. 3 is the response spectrum of the fluorescence signal, or "fingerprint", of the mixture of different sugars and yellow-and green-fluorescent CdTe quantum dots, respectively, under the optimum time of UV irradiation in example 2 of the present invention, wherein a: D-mannitol, b: D- (+) -xylose, c: L- (+) -arabinose, D: D-mannose, e: D- (+) galactose, f: D-fructose, g: D-glucose, K represents R of the sugar-CdTe complexG、GG、BG、IG、RY、GY、BY、IY，K0R representing CdTe quantum dotG0、GG0、BG0、IG0、RY0、GY0、BY0、IY0Wherein G and Y represent green and yellow fluorescent CdTe quantum dots, respectively;
FIG. 4 is a graph showing the results of discrimination of 7 different sugars based on Principal Component Analysis (PCA) treatment under optimum time conditions of ultraviolet irradiation in example 2 of the present invention, with a confidence interval of 81.581%, wherein a: D-mannitol, b: D- (+) -xylose, c: L- (+) -arabinose, D: D-mannose, e: D- (+) galactose, f: D-fructose, and g: D-glucose.
Detailed Description
The experimental procedures used in the following examples are conventional unless otherwise specified.
Reagents and consumables used in the following examples are commercially available, unless otherwise specified.
The fluorescence imaging instrument used in the following examples was a UV gel Bio-imaging System (ChemiDoc (TM) P.Biod.rad, America), the secondary water described herein was ultrapure water supplied from an ultrapure water generator (Elix5+ Millio-Q, Millipore, America). the oven used was an electrothermal forced air drying oven (WG L-230B, Tester instruments Inc. of Tianjin, China).
The surfaces of the CdTe quantum dots which emit yellow and green fluorescence are coated with thioglycolic acid and are water-soluble.
Reagent information needed for the synthesis of yellow and green fluorescent CdTe quantum dots in the following examples: tellurium powder, cadmium chloride (CdCl)2·2.5H2O) and thioglycolic acid (TGA) are purchased from Beijing Boyang Hongda science and technology Co., Ltd, sodium hydroxide (NaOH) is purchased from national pharmaceutical group chemical reagent Co., Ltd, and sodium borohydride (NaBH)4) Purchased from Gansu Jinbogao research Biotechnology, Inc., and nitrogen (99.995% pure) purchased from Suzhou Jinhong gas, Inc.
The information on the 7 sugars tested in the following examples, D-mannitol, D- (+) -xylose, L- (+) -arabinose, D-mannose, D- (+) galactose, D-fructose, and D-glucose were all purchased from Michelia biological Limited (Beijing, China) in Beijing Haenchidaceae.
Example 1
The synthesis of CdTe quantum dots emitting yellow and green fluorescence includes the following steps:
(1) placing 10m L ultrapure water in a three-neck round-bottom flask, introducing high-purity nitrogen to remove air, weighing 0.1276g tellurium powder and 0.10g sodium borohydride (NaBH)4) Quickly mixing the solution in ultrapure water, reacting under the protection of high-purity nitrogen until the solution becomes clear and is light pink to obtain a sodium hydrogen telluride (NaHTe) solution.
(2) Then 200m L m ultrapure water is put into a three-neck flask, 0.4567g cadmium chloride (CdCl) is added2·2.5H2O), adding 420 mu L thioglycolic acid (TGA), adjusting pH to 9.2 with sodium hydroxide (NaOH) particles, introducing high-purity nitrogen to remove air to obtain cadmium chloride-thioglycolic acid (CdCl)2-TGA) solution.
(3) Rapid mixing of tellurium under protection of high purity nitrogenSodium hydride (NaHTe) and cadmium chloride-thioglycolic acid (CdCl)2-TGA) solution to obtain an orange mixed solution.
(4) And respectively heating the mixed solution in an oven at 80 ℃ for 90min, cooling to obtain green fluorescent CdTe quantum dots, and heating for 105min to obtain yellow fluorescent CdTe quantum dots.
Example 2
The application method of CdTe quantum dots emitting yellow and green fluorescence for recognizing saccharides includes the following steps:
(1) preparation of sugar solution
In the experiment, 7 different sugars are selected as identification objects, namely D-mannitol, D- (+) -xylose, L- (+) -arabinose, D-mannose, D- (+) -galactose, D-fructose and D-glucose, and the 7 sugars are respectively weighed, respectively dissolved in ultrapure water, uniformly mixed and stored at 4 ℃ for later use, and the concentration of the sugar solution is 2.0mg/m L.
(2) Sensor constructed based on CdTe quantum dots irradiated by ultraviolet light for distinguishing and identifying 7 kinds of sugar
Uniformly mixing a CdTe quantum dot solution and a sugar solution in a certain proportion, placing the mixture in a centrifugal tube to enable the concentration of the final CdTe quantum dot to be 1.2mM and the concentration of sugar to be 1.0mg/m L, irradiating the CdTe-sugar mixed solution by using an ultraviolet lamp with the wavelength of 312 +/-10 nm for different times (0min, 10min, 20min, 30min and 40min), collecting the fluorescence intensity of the mixed solution by using an ultraviolet gel imaging system under the same ultraviolet wavelength, collecting the fluorescence color by using a common camera as shown in figure 2, and using the same amount of ultrapure water to replace the sugar solution as shown in figure 1 as a blank control, selecting the ultraviolet irradiation time with the optimal effect to carry out the next experiment, wherein the optimal time is 20min and 30 min.
(3) Acquisition and processing of multi-channel fluorescence data
Collecting and recording the different signals generated in the step (2), collecting the fluorescence color (R, G, B value) and the intensity value (I value) of the CdTe-sugar mixed solution and the blank control by using Photoshop CS2 software, and taking the signal ratio (K/K) of the signal of each CdTe-sugar sample to the blank0) And (5) further editing. The signal ratio was processed using Origin 9.0 to obtain the ratio of each saccharideFingerprint analysis, as shown in fig. 3, was performed on 7 sugars and the resulting signal ratios were subjected to Principal Component Analysis (PCA) using SPSS 22.0 software to further distinguish the 7 sugars, as shown in fig. 4.
Claims (7)
1. A CdTe quantum dot multichannel fluorescence sensor based on ultraviolet irradiation is characterized in that: the multi-channel fluorescence sensor can emit yellow fluorescence or green fluorescence under the condition of ultraviolet irradiation.
2. An application of a CdTe quantum dot multi-channel fluorescence sensor based on ultraviolet irradiation in identifying biochemical substances is characterized in that: the fluorescence color of the multi-channel fluorescence sensor changes under the irradiation of ultraviolet light, and different biochemical substances can interfere with the change of the fluorescence color to different degrees by combining with the multi-channel fluorescence sensor, so that the different biochemical substances can be distinguished and identified.
3. Use according to claim 2, characterized in that: the biochemical substances independently include saccharides, proteins and heavy metals.
4. The use according to claim 3, wherein the saccharide comprises one or more of D-mannitol, D- (+) -xylose, L- (+) -arabinose, D-mannose, D- (+) galactose, D-fructose and D-glucose.
5. A method for identifying biochemical substances by a CdTe quantum dot multichannel fluorescence sensor based on ultraviolet irradiation is characterized by comprising the following steps:
(1) dissolving several kinds of biochemical substances in pure water to prepare solutions;
(2) mixing yellow and green fluorescent CdTe quantum dots with the solutions prepared in step (1), respectively, irradiating with 312 + -10 nm ultraviolet lamp for 20-30min, observing and recording the fluorescent color and intensity at the same wavelength, using secondary water instead of sugar solution as blank control,
(3) the aim of distinguishing and identifying different biochemical substances is fulfilled by observing the change of the fluorescence color and the interference of different biochemical substances on the fluorescence intensity to different degrees.
6. The method for identifying the biochemical substances by the CdTe quantum dot multichannel fluorescence sensor based on ultraviolet irradiation as claimed in claim 5, wherein: the wavelength of ultraviolet light emitted by the ultraviolet lamp is 312 +/-10 nm.
7. The method for identifying biochemical substances by using the CdTe quantum dot multichannel fluorescence sensor based on ultraviolet irradiation as claimed in claim 5 or 6, wherein: the irradiation time of the ultraviolet lamp is 20-30 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010151907.3A CN111398226A (en) | 2020-03-06 | 2020-03-06 | CdTe quantum dot multi-channel fluorescence sensor based on ultraviolet irradiation and application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010151907.3A CN111398226A (en) | 2020-03-06 | 2020-03-06 | CdTe quantum dot multi-channel fluorescence sensor based on ultraviolet irradiation and application method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111398226A true CN111398226A (en) | 2020-07-10 |
Family
ID=71432301
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010151907.3A Pending CN111398226A (en) | 2020-03-06 | 2020-03-06 | CdTe quantum dot multi-channel fluorescence sensor based on ultraviolet irradiation and application method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111398226A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760096A (en) * | 2021-01-11 | 2021-05-07 | 浙江工业大学 | Design method and application of fluorescent quenching array sensor based on cadmium telluride quantum dots |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105911030A (en) * | 2016-04-05 | 2016-08-31 | 中国科学院合肥物质科学研究院 | Ratio fluorescence sensor and visualized detection method for glucose |
| CN106323927A (en) * | 2016-08-16 | 2017-01-11 | 江苏科技大学 | Multi-channel sensor capable of conducting synchronous detection for multiple protein based on CdTe quantum dot |
| CN110487759A (en) * | 2019-08-22 | 2019-11-22 | 东南大学 | For the double-colored Ratio-type quantum dot aeroge microsensor of glucose and its application |
-
2020
- 2020-03-06 CN CN202010151907.3A patent/CN111398226A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105911030A (en) * | 2016-04-05 | 2016-08-31 | 中国科学院合肥物质科学研究院 | Ratio fluorescence sensor and visualized detection method for glucose |
| CN106323927A (en) * | 2016-08-16 | 2017-01-11 | 江苏科技大学 | Multi-channel sensor capable of conducting synchronous detection for multiple protein based on CdTe quantum dot |
| CN110487759A (en) * | 2019-08-22 | 2019-11-22 | 东南大学 | For the double-colored Ratio-type quantum dot aeroge microsensor of glucose and its application |
Non-Patent Citations (2)
| Title |
|---|
| 苗恒等: "水溶性CdTe量子点的制备和表面修饰及表征", 《广东化工》 * |
| 马琳等: "基于CdTe量子点的三通道传感器对蛋白质的同步识别", 《光谱学与光谱分析》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760096A (en) * | 2021-01-11 | 2021-05-07 | 浙江工业大学 | Design method and application of fluorescent quenching array sensor based on cadmium telluride quantum dots |
| CN112760096B (en) * | 2021-01-11 | 2022-08-12 | 浙江工业大学 | Design method and application of fluorescent quenching array sensor based on cadmium telluride quantum dots |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111579665B (en) | UPLC/HRMS-based metabonomics relative quantitative analysis method | |
| CN102213714A (en) | Detection method for identifying natural taurine and synthesized taurine | |
| CN104830315A (en) | Bivalent copper ion fluorescent probe and preparation method and application thereof | |
| CN111982789B (en) | High-throughput detection method of metal ions and metal nanoparticles based on single-cell enrichment and single-cell mass spectrometry | |
| CN111458314A (en) | Method for identifying production place of dried orange peel based on autofluorescence-quenching fluorescence splicing spectrum | |
| Li et al. | A novel acid-sensitive quantum dot sensor array for the identification of Chinese baijiu | |
| CN111398226A (en) | CdTe quantum dot multi-channel fluorescence sensor based on ultraviolet irradiation and application method | |
| CN111999403A (en) | Gas explosion lung injury diagnosis system, serum marker screening method and lung injury action mechanism research method | |
| Hao et al. | Determination of serum selenium by hydride generation flame atomic absorption spectrometry | |
| CN113861175A (en) | Hg based on naphthalimide2+Fluorescent probe and preparation method thereof | |
| CN112630199B (en) | Alloy nano-cluster-based folic acid content detection method | |
| CN112285346B (en) | High-sensitivity HIV p24 detection method based on quantum dot selective cation exchange reaction | |
| CN101817838B (en) | Cadmium ion probe and synthesis method thereof | |
| CN109596580A (en) | Method based on glutamic-pyruvic transaminase in copper nanocluster fluorescence probe quantitative detection solution | |
| CN116908279A (en) | Method for monitoring metabolite reaction based on matrix-assisted laser desorption ionization mass spectrometry | |
| Udenfriend | Principles of fluorescence | |
| CN109053711B (en) | Probe compound for mercury ion detection and preparation method and application thereof | |
| CN107356577B (en) | Universal sulfotransferase activity analysis method | |
| CN112358485B (en) | Fluorescent probe for visual detection of copper ions in plant root system and preparation method thereof | |
| CN108997255A (en) | A kind of vinyl ethers Hg2+Fluorescence probe and its preparation method and application | |
| CN110526865B (en) | Bimolecular fluorescent compound for detecting HOCl and preparation and application thereof | |
| CN102004090A (en) | Design of off-on fluorescent molecular probe for selective recognition of Cd2+ | |
| Virani et al. | Mass cytometry: an evolution in ICP-MS enabling novel insights in single-cell biology | |
| CN108801961B (en) | Biotin detection method | |
| Chen et al. | Biosensors for single-cell metabolomic characterization |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200710 |