CN111387350A - Animal feed additive - Google Patents
Animal feed additive Download PDFInfo
- Publication number
- CN111387350A CN111387350A CN202010348013.3A CN202010348013A CN111387350A CN 111387350 A CN111387350 A CN 111387350A CN 202010348013 A CN202010348013 A CN 202010348013A CN 111387350 A CN111387350 A CN 111387350A
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- leather
- weight
- waste
- softened
- protein powder
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/10—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from hair, feathers, horn, skins, leather, bones, or the like
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Treatment And Processing Of Natural Fur Or Leather (AREA)
Abstract
The invention relates to an animal feed additive, which is leather protein powder and is prepared by the following steps: the method comprises the following steps: cleaning waste leather, soaking in warm water of 40-60 deg.C for 3-4h, taking out the soaked waste leather, cutting into pieces, soaking in softening liquid for 5-12h, softening the waste leather, and filtering to obtain softened leather; step two: placing the softened leather obtained in the step one in a hydrolytic enzyme solution, then pouring warm water at 30-40 ℃, and soaking in a rotary drum for 8-10 hours at the temperature of 25-35 ℃ to fully decompose the softened leather to obtain decomposed leather; step three: and D, drying the decomposed leather obtained in the step two in a dryer to obtain the leather protein powder. The leather protein powder is more nutritious because the leather protein is decomposed into micromolecular amino acid by hydrolyzing the softened leather with pectinase, carboxylesterase and lipase, and the leather protein powder is used as an animal feed additive, so that the amino acid content is high and the nutrition is rich.
Description
Technical Field
The invention relates to the technical field of animal feed correlation, in particular to an animal feed additive.
Background
Leather is animal skin which is obtained by physical and chemical processing such as unhairing, tanning and the like and is denatured and not easy to rot; along with the gradual improvement of living standard of people, the demand for leather clothes, leather trousers, leather shoes and leather furniture is larger and larger, the production amount of waste leather is larger and larger, and at present, most of the waste leather is directly thrown away, and only a small part of the waste leather is utilized.
At present, the waste leather is mainly used for animal feed after being treated at home and abroad, and collagen is extracted and used for food additives, pharmaceutical industry, clinical medicine, clinical treatment, cosmetics and the like. In the method for treating the tanning waste, the product still contains a certain amount of chromium and has certain pollution, for example, in an animal feed product prepared by the extract, the chromium is remained and enriched in the animal body after the animal eats and is transmitted through a food chain, and finally, the toxic injury is caused to people and livestock. In addition, the above tanning waste utilization process inevitably causes new environmental pollution, which is also a problem that is not negligible.
Disclosure of Invention
In order to solve the problems, the invention provides an animal feed additive, which can be used for changing waste leather into leather protein powder by an enzymolysis method and has rich amino acid content.
The invention aims to provide an animal feed additive, which is leather protein powder and is prepared by the following steps:
step one, softening: cleaning waste leather, soaking in warm water of 40-60 deg.C for 3-4h, taking out the soaked waste leather, cutting into pieces, soaking in softening liquid for 5-12h, softening the waste leather, and filtering to obtain softened leather;
step two, decomposition: placing the softened leather obtained in the step one in a hydrolytic enzyme solution, then pouring warm water at 30-40 ℃, and soaking in a rotary drum for 8-10 hours at the temperature of 25-35 ℃ to fully decompose the softened leather to obtain decomposed leather;
step three, drying: and D, drying the decomposed leather obtained in the step two in a dryer to obtain the leather protein powder.
In some embodiments of the present invention, the softening liquid in the first step is prepared by mixing 2 to 5 parts by weight of acetic acid solution, 5 to 6 parts by weight of carrageenan, 0.5 to 1 part by weight of squalane, 6 to 7 parts by weight of stearic acid and 50 to 60 parts by weight of water.
In some embodiments of the invention, the acetic acid solution contains species of acetic acid molecules in a concentration of 1-3 mol/L.
In some embodiments of the present invention, the weight ratio of the waste leather, the warm water and the softening liquid in the first step is 1: (5-10): (5-10).
In some embodiments of the present invention, the concentration of the substance of the hydrolase in the second step is 1-6 mol/L.
In some embodiments of the present invention, the weight ratio of the second step softening leather to the hydrolytic enzyme solution is 1: (4-10).
Compared with the prior art, the invention has the beneficial effects that: the leather protein powder is more nutritious because the leather protein is decomposed into micromolecular amino acid by hydrolyzing the softened leather with pectinase, carboxylesterase and lipase, and the leather protein powder is used as an animal feed additive, so that the amino acid content is high and the nutrition is rich. And the preparation process of the leather protein powder is as follows: the softening agent adopted by the invention is prepared by mixing 2-5 parts by weight of acetic acid solution, 5-6 parts by weight of carrageenan, 0.5-1 part by weight of squalane, 6-7 parts by weight of stearic acid and 50-60 parts by weight of water, wherein the acetic acid can effectively remove chromium element in leather products, so that the prepared leather protein powder has little pollution to the environment.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below so that those skilled in the art can better understand the advantages and features of the present invention, and thus the scope of the present invention will be more clearly defined. The embodiments described herein are only a few embodiments of the present invention, rather than all embodiments, and all other embodiments that can be derived by one of ordinary skill in the art without inventive faculty based on the embodiments described herein are intended to fall within the scope of the present invention.
Example 1
An animal feed additive, which is leather protein powder, and the leather protein powder is prepared by the following steps:
step one, softening: cleaning 1kg of waste leather, soaking the waste leather in warm water of 7.5kg at 50 ℃ for 3.5h, taking out the soaked waste leather, cutting the waste leather into pieces, soaking the waste leather in softening liquid of 7.5kg for 9h, softening the waste leather, and filtering to obtain 3.5kg of softened leather;
step two, decomposition, namely putting 3.5kg of the softened leather obtained in the step one into 3 mol/L of 12mg of hydrolytic enzyme (pectinase) solution, then pouring warm water at 35 ℃, and performing rotary drum soaking at the temperature of 20 ℃ for 9 hours to fully decompose the softened leather to obtain 0.75kg of decomposed leather;
step three, drying: drying 0.75kg of decomposed leather obtained in the step two in a dryer at 110 ℃ for 1.5h to obtain leather protein powder;
the softening liquid in the first step is prepared by mixing 3.5 parts by weight of acetic acid solution (the mass concentration of substances containing acetic acid molecules is 2 mol/L), 5.5 parts by weight of carrageenan, 0.75 part by weight of squalane, 6.5 parts by weight of stearic acid and 55 parts by weight of water.
Example 2
An animal feed additive, which is leather protein powder, and the leather protein powder is prepared by the following steps:
step one, softening: cleaning 1kg of waste leather, soaking the waste leather in warm water of 40 ℃ and 5kg for 4h, taking out the soaked waste leather, cutting the waste leather into pieces, soaking the cut waste leather in softening liquid of 5kg for 12h, softening the waste leather, and filtering to obtain 2kg of softened leather;
step two, decomposing, namely putting 2kg of the softened leather obtained in the step one in 6 mol/L and 50mg of hydrolytic enzyme (carboxylesterase) solution, then pouring warm water at 30 ℃, and performing rotary drum soaking at the temperature of 25 ℃ for 10 hours to fully decompose the softened leather to obtain 0.5kg of decomposed leather;
step three, drying: drying 0.5kg of decomposed leather obtained in the step two in a dryer at 100 ℃ for 2h to obtain leather protein powder;
the softening liquid in the first step is prepared by mixing 2 parts by weight of acetic acid solution (the mass concentration of acetic acid molecules is 3 mol/L), 5 parts by weight of carrageenan, 0.5 part by weight of squalane, 6 parts by weight of stearic acid and 60 parts by weight of water.
Example 3
An animal feed additive, which is leather protein powder, and the leather protein powder is prepared by the following steps:
step one, softening: cleaning 1kg of waste leather, soaking the waste leather in 10kg of warm water at 60 ℃ for 3h, taking out the soaked waste leather, cutting the waste leather into pieces, soaking the cut waste leather in 10kg of softening liquid for 5h, softening the waste leather, and filtering to obtain 5kg of softened leather;
step two, decomposing, namely putting 5kg of softened leather obtained in the step one in 1 mol/L, 8mg of hydrolytic enzyme (lipase) solution, then pouring warm water at 40 ℃, and performing rotary drum soaking for 8 hours at 35 ℃ to fully decompose the softened leather to obtain 1kg of decomposed leather;
step three, drying: drying 1kg of decomposed leather obtained in the step two in a dryer at 120 ℃ for 1h to obtain leather protein powder;
the softening liquid in the first step is prepared by mixing 5 parts by weight of acetic acid solution (the mass concentration of acetic acid molecules is 1 mol/L), 6 parts by weight of carrageenan, 1 part by weight of squalane, 7 parts by weight of stearic acid and 50 parts by weight of water.
Example 4
An animal feed additive, which is leather protein powder, and the leather protein powder is prepared by the following steps:
step one, softening: cleaning 1kg of waste leather, soaking the waste leather in 6kg of warm water at 45 ℃ for 3h, taking out the soaked waste leather, cutting the waste leather into pieces, soaking the cut waste leather in 8kg of softening liquid for 5h, softening the waste leather, and filtering to obtain 2.5kg of softened leather;
step two, decomposing, namely putting 2.5kg of the softened leather obtained in the step one into 2 mol/L and 10mg of a solution of a hydrolase (pectinase: carboxylesterase ═ 1: 1), then pouring warm water at 30 ℃, and performing rotary drum soaking for 8 hours at the temperature of 35 ℃ so that the softened leather is fully decomposed to obtain 0.9kg of decomposed leather;
step three, drying: drying 0.9kg of decomposed leather obtained in the step two in a dryer at 120 ℃ for 1h to obtain leather protein powder;
the softening liquid in the first step is prepared by mixing 2 parts by weight of acetic acid solution (the mass concentration of acetic acid molecules is 3 mol/L), 5 parts by weight of carrageenan, 0.5 part by weight of squalane, 6 parts by weight of stearic acid and 58 parts by weight of water.
Example 5
An animal feed additive, which is leather protein powder, and the leather protein powder is prepared by the following steps:
step one, softening: cleaning 1kg of waste leather, soaking the waste leather in warm water of 55 ℃ and 8kg for 4h, taking out the soaked waste leather, cutting the waste leather into pieces, soaking the cut waste leather in softening liquid of 6kg for 10h, softening the waste leather, and filtering to obtain 3kg of softened leather;
step two, decomposing, namely putting 3kg of the softened leather obtained in the step one into 2 mol/L and 30mg of hydrolytic enzyme (pectinase: lipase: 1) solution, then pouring warm water at 40 ℃, and performing rotary drum soaking at the temperature of 25 ℃ for 10 hours to fully decompose the softened leather to obtain 0.56kg of decomposed leather;
step three, drying: drying 0.56kg of decomposed leather obtained in the step two in a dryer at 100 ℃ for 2h to obtain leather protein powder;
the softening liquid in the first step is prepared by mixing 4 parts by weight of acetic acid solution (the mass concentration of acetic acid molecules is 2.5 mol/L), 5 parts by weight of carrageenan, 0.8 part by weight of squalane, 6 parts by weight of stearic acid and 50 parts by weight of water.
Example 6
An animal feed additive, which is leather protein powder, and the leather protein powder is prepared by the following steps:
step one, softening: cleaning 1kg of waste leather, soaking the waste leather in 10kg of warm water at 60 ℃ for 3h, taking out the soaked waste leather, cutting the waste leather into pieces, soaking the cut waste leather in 10kg of softening liquid for 5h, softening the waste leather, and filtering to obtain 5kg of softened leather;
step two, decomposing, namely putting 5kg of softened leather obtained in the step one in 1 mol/L, 8mg of hydrolytic enzyme (lipase) solution, then pouring warm water at 40 ℃, and performing rotary drum soaking for 8 hours at 35 ℃ to fully decompose the softened leather to obtain 1kg of decomposed leather;
step three, drying: drying 1kg of decomposed leather obtained in the step two in a dryer at 120 ℃ for 1h to obtain leather protein powder;
the softening liquid in the first step is prepared by mixing 3.5 parts by weight of acetic acid solution (the mass concentration of substances containing acetic acid molecules is 2 mol/L), 5.5 parts by weight of carrageenan, 0.75 part by weight of squalane, 6.5 parts by weight of stearic acid and 55 parts by weight of water.
Example 7
An animal feed additive, which is leather protein powder, and the leather protein powder is prepared by the following steps:
step one, softening: cleaning 1kg of waste leather, soaking the waste leather in warm water of 7.5kg at 50 ℃ for 3.5h, taking out the soaked waste leather, cutting the waste leather into pieces, soaking the waste leather in softening liquid of 7.5kg for 9h, softening the waste leather, and filtering to obtain 3.5kg of softened leather;
step two, decomposition, namely putting 3.5kg of the softened leather obtained in the step one into 3 mol/L of 12mg of hydrolytic enzyme (pectinase) solution, then pouring warm water at 35 ℃, and performing rotary drum soaking at the temperature of 20 ℃ for 9 hours to fully decompose the softened leather to obtain 0.75kg of decomposed leather;
step three, drying: drying 0.75kg of decomposed leather obtained in the step two in a dryer at 110 ℃ for 1.5h to obtain leather protein powder;
the softening liquid in the first step is prepared by mixing 3.5 parts by weight of acetic acid solution (the mass concentration of substances containing acetic acid molecules is 2 mol/L), 5.5 parts by weight of carrageenan, 0.75 part by weight of squalane, 6.5 parts by weight of stearic acid and 55 parts by weight of water.
Example 8
An animal feed additive, which is leather protein powder, and the leather protein powder is prepared by the following steps:
step one, softening: cleaning 1kg of waste leather, soaking the waste leather in warm water of 7.5kg at 50 ℃ for 3.5h, taking out the soaked waste leather, cutting the waste leather into pieces, soaking the waste leather in softening liquid of 7.5kg for 9h, softening the waste leather, and filtering to obtain 3.5kg of softened leather;
step two, decomposition, namely putting 3.5kg of the softened leather obtained in the step one into 3 mol/L of 12mg of hydrolytic enzyme (pectinase) solution, then pouring warm water at 35 ℃, and performing rotary drum soaking at the temperature of 20 ℃ for 9 hours to fully decompose the softened leather to obtain 0.75kg of decomposed leather;
step three, drying: drying 0.75kg of decomposed leather obtained in the step two in a dryer at 110 ℃ for 1.5h to obtain leather protein powder;
the softening liquid in the first step is prepared by mixing 5 parts by weight of acetic acid solution (the mass concentration of acetic acid molecules is 1 mol/L), 6 parts by weight of carrageenan, 1 part by weight of squalane, 7 parts by weight of stearic acid and 50 parts by weight of water.
Example 9
An animal feed additive, which is leather protein powder, and the leather protein powder is prepared by the following steps:
step one, softening: cleaning 1kg of waste leather, soaking the waste leather in warm water of 40 ℃ and 5kg for 4h, taking out the soaked waste leather, cutting the waste leather into pieces, soaking the cut waste leather in softening liquid of 5kg for 12h, softening the waste leather, and filtering to obtain 2kg of softened leather;
step two, decomposing, namely putting 2kg of the softened leather obtained in the step one in 6 mol/L and 50mg of hydrolytic enzyme (carboxylesterase) solution, then pouring warm water at 30 ℃, and performing rotary drum soaking at the temperature of 25 ℃ for 10 hours to fully decompose the softened leather to obtain 0.5kg of decomposed leather;
step three, drying: drying 0.5kg of decomposed leather obtained in the step two in a dryer at 100 ℃ for 2h to obtain leather protein powder;
the softening liquid in the first step is prepared by mixing 3.5 parts by weight of acetic acid solution (the mass concentration of substances containing acetic acid molecules is 2 mol/L), 5.5 parts by weight of carrageenan, 0.75 part by weight of squalane, 6.5 parts by weight of stearic acid and 55 parts by weight of water.
Examples of the experiments
The contents of chromium and amino acids in the leather protein powder prepared in examples 1-9 were determined, and the details are shown in table 1:
TABLE 1
As shown in Table 1, the leather protein prepared by the method of the invention has no content of chromium element detected, and can be used as an additive of plant fertilizer; and the amino acid content is rich, the nutrition is rich, and the feed additive can be used as an animal feed additive.
The present invention provides only a method for recycling waste leather, and a plurality of methods and ways for implementing the technical scheme, and the above description is only a preferred embodiment of the present invention, it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention, and each component not explicitly described in the embodiment can be implemented by the prior art.
Claims (6)
1. An animal feed additive is characterized in that the additive is leather protein powder, and the leather protein powder is prepared by the following steps:
the method comprises the following steps: cleaning waste leather, soaking in warm water of 40-60 deg.C for 3-4h, taking out the soaked waste leather, cutting into pieces, soaking in softening liquid for 5-12h, softening the waste leather, and filtering to obtain softened leather;
step two: placing the softened leather obtained in the step one in a hydrolytic enzyme solution, then pouring warm water at 30-40 ℃, and soaking in a rotary drum for 8-10 hours at the temperature of 25-35 ℃ to fully decompose the softened leather to obtain decomposed leather;
step three: and D, drying the decomposed leather obtained in the step two in a dryer to obtain the leather protein powder.
2. The animal feed additive according to claim 1, wherein the softening solution in the first step is prepared by mixing 2-5 parts by weight of acetic acid solution, 5-6 parts by weight of carrageenan, 0.5-1 part by weight of squalane, 6-7 parts by weight of stearic acid and 50-60 parts by weight of water.
3. The animal feed additive according to claim 1, wherein the acetic acid solution contains acetic acid molecules in a concentration of 1 to 3 mol/L.
4. The animal feed additive of claim 1, wherein: the weight ratio of the waste leather, the warm water and the softening liquid in the step one is 1: (5-10): (5-10).
5. The animal feed additive of claim 1, wherein the concentration of the hydrolase substance in the hydrolase solution in the second step is 1 to 6 mol/L.
6. The animal feed additive of claim 1, wherein: the weight ratio of the softened leather in the second step to the hydrolytic enzyme solution is 1 kg: (4-10) mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010348013.3A CN111387350A (en) | 2020-04-28 | 2020-04-28 | Animal feed additive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010348013.3A CN111387350A (en) | 2020-04-28 | 2020-04-28 | Animal feed additive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111387350A true CN111387350A (en) | 2020-07-10 |
Family
ID=71412533
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010348013.3A Withdrawn CN111387350A (en) | 2020-04-28 | 2020-04-28 | Animal feed additive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111387350A (en) |
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2020
- 2020-04-28 CN CN202010348013.3A patent/CN111387350A/en not_active Withdrawn
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Application publication date: 20200710 |
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