CN111387160A - Method for capturing and identifying field arthropods - Google Patents
Method for capturing and identifying field arthropods Download PDFInfo
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- CN111387160A CN111387160A CN202010194181.1A CN202010194181A CN111387160A CN 111387160 A CN111387160 A CN 111387160A CN 202010194181 A CN202010194181 A CN 202010194181A CN 111387160 A CN111387160 A CN 111387160A
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- arthropods
- capturing
- identifying
- sampling
- big data
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/14—Catching by adhesive surfaces
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M23/00—Traps for animals
- A01M23/005—Traps for animals with sticky surfaces
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
Abstract
The invention belongs to the technical field of agriculture, and relates to a method for capturing and identifying field limb animals, which comprises the following steps: 1) preparing a readily degradable pest sticker solution; 2) coating the easily degradable armyworm agent solution prepared in the step 1) on the inner surface of a sampling container; the sampling container comprises a sampling basin and a cover plate matched with the sampling basin; 3) moving the sampling container to the vicinity of the plant, enabling the sampling pot and the cover plate to be matched with each other to clamp the plant and slightly shake the plant, and capturing and collecting arthropods near the plant; 4) and identifying the captured arthropods. The invention provides a field arthropod capturing and identifying method which is free of toxicity to organisms and environment, convenient for capturing arthropods and capable of improving the accuracy of experiments.
Description
Technical Field
The invention belongs to the technical field of agriculture, relates to an animal capturing and identifying method, and particularly relates to a field limb animal capturing and identifying method.
Background
Biodiversity is the condition on which human beings live, is the basis of sustainable development of the economy and society, and is the guarantee of ecological safety and food safety. At present, research and protection on the diversity of the limbs and animals become a hot problem of the current society. The phylum arthropoda is the largest one in the animal world, and about 120 thousands of existing species account for 80% of the whole number of animals, and the distribution is extremely wide, including farmland ecosystem and natural ecosystem closely related to human production. At present, arthropods need to be sampled to obtain relevant parameters of arthropod populations and communities when evaluating the control effect of pesticides on rice pests, prediction and forecast of farmland pests, safety evaluation of transgenic plants and biological diversity of an ecological system. However, arthropod sampling methods are various, and there is still controversy as to which sampling method should be used in field arthropod investigation. The current commonly used field arthropod investigation methods comprise a direct observation method, a trematode method, a pot shooting method, a Marfan net method, a net sweeping method, a trap method and the like, and the following defects generally exist: 1) a lethality survey; 2) difficult to investigate and catch arthropods; 3) the adopted catching device is complex and is not easy to operate; 4) the sampling amount is not complete.
Disclosure of Invention
In order to solve the technical problems in the background art, the invention provides a field arthropod capturing and identifying method which is free of toxicity to organisms and environment, convenient for capturing arthropods and capable of improving the accuracy of experiments.
In order to achieve the purpose, the invention adopts the following technical scheme:
a field arthropod capture identification method, comprising the following steps:
1) preparing a readily degradable pest sticker solution;
2) coating the easily degradable armyworm agent solution prepared in the step 1) on the inner surface of a sampling container; the sampling container comprises a sampling basin and a cover plate matched with the sampling basin;
3) moving the sampling container to the vicinity of the plant, enabling the sampling pot and the cover plate to be matched with each other to clamp the plant and slightly shake the plant, and capturing and collecting arthropods near the plant;
4) and identifying the captured arthropods.
Preferably, the easily degradable adhesive used in step 1) of the present invention is an aqueous solution of natural sugar.
Preferably, the easily degradable adhesive used in step 1) of the present invention is a water saturated solution of natural sugar.
Preferably, the natural sugar used in the present invention is one or a combination of sucrose, glucose, fructose and honey.
Preferably, the identification in step 4) employed in the present invention is a visual identification.
Preferably, the identification in step 4) employed in the present invention further includes big data identification after naked eye identification.
Preferably, the specific implementation manner of big data identification adopted by the invention is as follows: the method comprises the steps of photographing captured arthropods, sending photographing results to a big data processing center or a cloud big data server, and carrying out background big data identification through the big data processing center or cloud big data identification through the cloud big data server.
Preferably, the method for capturing and identifying arthropods in the field used in the present invention further comprises, after step 4):
5) releasing the identified arthropods or carrying out laboratory research on the captured and collected arthropods according to the requirements of subsequent experiments; the specific implementation mode of the release is as follows: after the identification is finished, diluting the inner surface of the sampling container by water, so that the viscosity of the easily degradable armyworm agent solution on the inner surface of the sampling container is reduced, and the stuck animals are released.
Preferably, the method for capturing and identifying arthropods in the field used in the present invention further comprises, after step 5):
6) recycling the easily degradable pest sticker solution on the inner surface of the sampling container; the concrete implementation mode of recycling is as follows: and recovering the diluted easily-degradable armyworm solution, filtering and concentrating for later use.
The invention has the advantages that:
1) the invention adopts various viscous sugars to prepare the easily degradable armyworm agent solution, and the viscosity can be adjusted by changing the concentration of solute according to the requirement; the easily degradable insect sticker solution is formed by mixing water solutions or water solutions of natural substances such as cane sugar, glucose, fructose, honey and the like, belongs to biological and environment-friendly materials, has no toxicity to organisms, can change the concentration of a solute according to needs to adjust the viscosity, and generally adopts a saturated solution; meanwhile, as the easily degradable armyworm solution adopts natural sugar, the sweet taste of the armyworm solution is attractive and comforting to the arthropods;
2) the round pot and the cover plate for storing the arthropods can select different sizes according to different captured sampling objects, so that the arthropods of various types can be conveniently captured by holding the pot and the cover plate, and the operation is convenient;
3) according to the invention, a shooting device can be adopted to shoot and record the captured arthropod, and meanwhile, the shooting information is transmitted back to the big data processing center or the cloud big data server to carry out network big data comparison, so that the real-time performance and the effectiveness of the data are enhanced;
4) the arthropods which are identified can be released without damage, or the arthropods which are difficult to identify can be taken back, so that identification and statistics are convenient, or healthy living arthropods are collected according to the experiment requirements for subsequent experiments; in addition, healthy living arthropods can be captured according to the experiment requirements for subsequent experiments;
5) after the pesticide is used, the easily degradable pest sticker solution can be diluted by adding water, arthropods are released, the diluted easily degradable pest sticker solution is filtered and recovered, and the pesticide is repeatedly used after being concentrated, so that the economy is obvious;
6) the method is remarkably superior to the existing various methods in investigation efficiency, is very suitable for the investigation and research fields of various arthropods, and can remarkably improve the accuracy of the experiment.
Drawings
FIG. 1 is a diagram showing a comparison of different capture identification methods.
Detailed Description
The invention provides a field arthropod capturing and identifying method, which comprises the following steps:
1) preparing an easily degradable insect sticker solution, wherein the easily degradable insect sticker solution is a water solution of natural sugar, especially a water saturated solution of natural sugar; the natural sugar adopted by the invention is one or the combination of sucrose, glucose, fructose and honey;
2) coating the easily degradable armyworm agent solution prepared in the step 1) on the inner surface of a sampling container; the sampling container comprises a sampling basin and a cover plate matched with the sampling basin;
3) moving the sampling container to the vicinity of the plant, enabling the sampling pot and the cover plate to be matched with each other to clamp the plant and slightly shake the plant, and capturing and collecting arthropods near the plant;
4) and (3) identifying the captured arthropods, wherein the identification is visual identification and/or big data identification. When the captured arthropods are familiar varieties, the arthropods can be directly identified by naked eyes and accurate information can be obtained; when the captured arthropods are of an unfamiliar or indeterminate breed, the big data identification can be used directly or carried out again on the basis of the naked eye identification. When big data authentication is adopted, the concrete implementation mode of big data authentication is as follows: the method comprises the steps of photographing captured arthropods, sending photographing results to a big data processing center or a cloud big data server, and carrying out background big data identification through the big data processing center or cloud big data identification through the cloud big data server.
5) Releasing the identified arthropods or carrying out laboratory research on the captured and collected arthropods according to the requirements of subsequent experiments; the specific implementation mode of the birth control is as follows: after the identification is finished, diluting the inner surface of the sampling container by water, so that the viscosity of the easily degradable armyworm agent solution on the inner surface of the sampling container is reduced, and the stuck animals are released.
6) Recycling the easily degradable pest sticker solution on the inner surface of the sampling container; the concrete implementation mode of recycling is as follows: and recovering the diluted easily-degradable armyworm solution, filtering and concentrating for later use.
The insect sticking agent is prepared by uniformly coating water solutions of easily degradable and environment-friendly natural substances, such as cane sugar, glucose, fructose, honey and the like, on the inner wall of a round basin, and catching and collecting wild arthropods by matching with the slapping of a round cover plate and the closed space. And then the cover plate is opened to identify or photograph the stuck animal, and the usable cotton swab difficult to identify on site is picked into the storage tube and is brought back to the laboratory. After the identification is finished, water can be sprayed on the wall of the basin by a watering can, so that the sugar solution is diluted to reduce the viscosity of the sugar solution, and the sugar solution is filtered by a screen mesh to finally release the stuck animals, and the insect sticking agent can be recycled. Compared with the traditional method, the method can be used for capturing the arthropods with strong driving force (difficult to capture) to identify, does not damage the captured objects in the identification process, can be used for capturing the arthropods for various subsequent experiments, belongs to a non-lethal investigation method, can provide more accurate and reliable data for the experiments needing to be investigated for multiple times in the same sample, and can also be used for taking pictures and keeping high-definition animal pictures and keeping or carrying out online big data comparison. The natural armyworm agent has no toxicity to animals, is nutritious and comfortable, can be recycled after being used, can be degraded quickly even if accidentally entering the environment, has no pollution to the environment, and cannot interfere the performance of other experiments.
The method provided by the invention is explained in detail below by taking specific applications as examples:
example (b):
1) test sites and subjects:
test time: 8/12/2019
Test site: the test site is located in provincial agricultural high-tech garden of Zhejiang university in Changxing county of Huzhou of Zhejiang province, and is 30 degrees in north latitude 52 'and 119 degrees in east longitude 37'. Designing a random block: cell area 150m2And 3 repetitions.
Subject: rice Xiushui 134
The management mode is as follows: transplanting in the density of 14 holes/5 day in 6 months and 20 daysm2And 1 seedling per hole. And (4) managing fertilizer and water according to a local conventional mode, carrying out artificial weeding and applying no pesticide.
2) The experimental method comprises the following steps:
2.1) the method adopted by the invention:
the detailed operation flow is as follows: the pest sticking agent with proper concentration (generally saturated solution of corresponding solute) is prepared, after the pest sticking agent reaches a sampling point, the pest sticking agent is uniformly smeared on the inner surface of a sampling basin, a plant to be sampled is selected, a cover plate and the sampling basin are matched to clamp and slightly shake the plant at proper time, some arthropods with strong flying and moving capabilities can fall into the basin firstly and are stuck, then the plant is continuously flapped by the cover plate, and other arthropods can fall into the basin and are stuck. After sufficient collection, the cover plate is opened, arthropods stuck to the bottom of the basin are identified and counted, and the arthropods can be photographed or collected to bring the living bodies back indoors. After the operation is finished, spraying water or slowly pouring the solution into a basin, diluting the insect-sticking agent, releasing the stuck arthropods, and filtering and recovering the liquid for reuse.
2.2) other methods of investigation:
including direct observation, trematode and pot swatter methods all operate according to relevant standards.
3) Results of the experiment
As shown in fig. 1, arthropods investigated using the present invention are significantly higher in both quantity and diversity than other various methods. The method is obviously superior to the existing various methods in investigation efficiency, has various advantages of greenness, no damage and the like, is very suitable for the investigation and research fields of various arthropods, and can obviously improve the accuracy of experiments.
Claims (9)
1. A method for capturing and identifying limb animals in a field is characterized by comprising the following steps: the method for capturing and identifying the field arthropods comprises the following steps:
1) preparing a readily degradable pest sticker solution;
2) coating the easily degradable armyworm agent solution prepared in the step 1) on the inner surface of a sampling container; the sampling container comprises a sampling basin and a cover plate matched with the sampling basin;
3) moving the sampling container to the vicinity of the plant, enabling the sampling pot and the cover plate to be matched with each other to clamp the plant and slightly shake the plant, and capturing and collecting arthropods near the plant;
4) and identifying the captured arthropods.
2. The method for capturing and identifying arthropods in a field according to claim 1, wherein: the easily degradable insect sticking agent in the step 1) is a water solution of natural sugar.
3. The method for capturing and identifying arthropods in the field according to claim 2, wherein: the easily degradable insect sticking agent in the step 1) is a water saturated solution of natural sugar.
4. The method for capturing and identifying arthropods in the field according to claim 3, wherein: the natural sugar is one or combination of sucrose, glucose, fructose and honey.
5. The method for capturing and identifying arthropods in the field according to claim 1 or 2 or 3 or 4, wherein: the identification in the step 4) is visual identification.
6. The method for capturing and identifying arthropods in the field according to claim 5, wherein: the authentication in the step 4) further comprises big data authentication after naked eye authentication.
7. The method for capturing and identifying arthropods in a field according to claim 6, wherein: the specific implementation mode of the big data identification is as follows: the method comprises the steps of photographing captured arthropods, sending photographing results to a big data processing center or a cloud big data server, and carrying out background big data identification through the big data processing center or cloud big data identification through the cloud big data server.
8. The method for capturing and identifying arthropods in the field according to claim 1 or 2 or 3 or 4, wherein: the field arthropod capture identification method further comprises, after step 4):
5) releasing the identified arthropods or carrying out laboratory research on the captured and collected arthropods according to the requirements of subsequent experiments; the specific implementation mode of the release is as follows: after the identification is finished, diluting the inner surface of the sampling container by water, so that the viscosity of the easily degradable armyworm agent solution on the inner surface of the sampling container is reduced, and the stuck animals are released.
9. The method for capturing and identifying arthropods in a field according to claim 8, wherein: the field arthropod capture identification method further comprises, after step 5):
6) recycling the easily degradable pest sticker solution on the inner surface of the sampling container; the concrete implementation mode of recycling is as follows: and recovering the diluted easily-degradable armyworm solution, filtering and concentrating for later use.
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| CN202010194181.1A CN111387160A (en) | 2020-03-18 | 2020-03-18 | Method for capturing and identifying field arthropods |
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| CN202010194181.1A CN111387160A (en) | 2020-03-18 | 2020-03-18 | Method for capturing and identifying field arthropods |
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Citations (6)
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| US20050025357A1 (en) * | 2003-06-13 | 2005-02-03 | Landwehr Val R. | Method and system for detecting and classifying objects in images, such as insects and other arthropods |
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| CN104686473A (en) * | 2015-03-10 | 2015-06-10 | 中国农业科学院植物保护研究所 | Insect quantity survey method |
| CN105724348A (en) * | 2016-01-28 | 2016-07-06 | 福建省农业科学院植物保护研究所 | Rice planthopper field capturing and collecting device and use method thereof |
| CN106132198A (en) * | 2014-02-02 | 2016-11-16 | 西汉姆有限公司 | The attractant in nipa palm source and bait station |
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2020
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Patent Citations (6)
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| US20050025357A1 (en) * | 2003-06-13 | 2005-02-03 | Landwehr Val R. | Method and system for detecting and classifying objects in images, such as insects and other arthropods |
| CN201101078Y (en) * | 2007-09-29 | 2008-08-20 | 孙祥良 | Investigation insect capturing device for homoptera and glossata insect |
| CN102696565A (en) * | 2012-06-04 | 2012-10-03 | 广东省昆虫研究所 | New method for surveying population density of microarthropods such as panonychus citri mcgregor, predatory mites and diaphorina citri kuwayama |
| CN106132198A (en) * | 2014-02-02 | 2016-11-16 | 西汉姆有限公司 | The attractant in nipa palm source and bait station |
| CN104686473A (en) * | 2015-03-10 | 2015-06-10 | 中国农业科学院植物保护研究所 | Insect quantity survey method |
| CN105724348A (en) * | 2016-01-28 | 2016-07-06 | 福建省农业科学院植物保护研究所 | Rice planthopper field capturing and collecting device and use method thereof |
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Application publication date: 20200710 |