CN111380742A - Sample loading detection system and detection method for blood vitamin D pretreatment - Google Patents
Sample loading detection system and detection method for blood vitamin D pretreatment Download PDFInfo
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- CN111380742A CN111380742A CN202010170581.9A CN202010170581A CN111380742A CN 111380742 A CN111380742 A CN 111380742A CN 202010170581 A CN202010170581 A CN 202010170581A CN 111380742 A CN111380742 A CN 111380742A
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- 238000001514 detection method Methods 0.000 title claims abstract description 54
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 title claims abstract description 35
- 229930003316 Vitamin D Natural products 0.000 title claims abstract description 34
- 235000019166 vitamin D Nutrition 0.000 title claims abstract description 34
- 239000011710 vitamin D Substances 0.000 title claims abstract description 34
- 150000003710 vitamin D derivatives Chemical class 0.000 title claims abstract description 34
- 229940046008 vitamin d Drugs 0.000 title claims abstract description 34
- 239000008280 blood Substances 0.000 title claims abstract description 21
- 210000004369 blood Anatomy 0.000 title claims abstract description 21
- 238000010828 elution Methods 0.000 claims abstract description 71
- 238000005070 sampling Methods 0.000 claims abstract description 45
- 239000000523 sample Substances 0.000 claims description 148
- 238000007789 sealing Methods 0.000 claims description 23
- 239000012156 elution solvent Substances 0.000 claims description 11
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 239000012488 sample solution Substances 0.000 claims description 7
- 238000007405 data analysis Methods 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 239000004020 conductor Substances 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 abstract description 5
- 239000007769 metal material Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108010004103 Chylomicrons Proteins 0.000 description 1
- UCTLRSWJYQTBFZ-UHFFFAOYSA-N Dehydrocholesterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)CCCC(C)C)CCC33)C)C3=CC=C21 UCTLRSWJYQTBFZ-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001841 cholesterols Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 235000005282 vitamin D3 Nutrition 0.000 description 1
- 239000011647 vitamin D3 Substances 0.000 description 1
- 229940021056 vitamin d3 Drugs 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/08—Preparation using an enricher
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/16—Injection
Abstract
The invention discloses a sample loading detection system and a detection method for blood vitamin D pretreatment, wherein the sample loading detection system for blood vitamin D pretreatment comprises: the device comprises a to-be-detected sample placing unit, a sample enrichment sampling needle, a sample elution sample loading needle, a sample elution unit, a single quadrupole rod quality detector and a data processing unit. The sample to be detected containing vitamin D is enriched through the sample enrichment sampling needle, after the sample is enriched through the sample enrichment sampling needle, the sample to be detected is sent to the sample elution sampling needle for elution, the sample is ionized under the action of a high-voltage power supply, the sample subjected to ionization treatment is sent to the single quadrupole rod quality detector for detection, the result is processed and analyzed through the data processing unit, and a detection report is given. The invention can realize the rapid detection of blood vitamin D, realize high-efficiency analysis and accurately and rapidly obtain a detection report.
Description
Technical Field
The invention relates to the field of analysis and detection, in particular to a sample loading detection system and a detection method for blood vitamin D pretreatment.
Background
Vitamin D (VD for short) is a fat-soluble vitamin and belongs to steroid derivatives. In humans, vitamin D plays an important role in maintaining the health of cells and is also a prerequisite for calcium absorption. On one hand, the vitamin D can be from diet, mainly from animal food such as fish liver, egg yolk and the like, and is absorbed from small intestine in the presence of bile after being ingested and is transported into blood in the form of chylomicron; on the other hand, the cholesterol derivative 7-dehydrocholesterol in the human body can be converted into cholecalciferol under the irradiation of sunlight or ultraviolet rays, namely, the endogenous vitamin D.
The current detection methods for vitamin D in blood include high performance liquid chromatography, gas chromatography, spectrophotometry and the like. However, the methods have the problems of poor specificity, complicated sample pretreatment, change of vitamin content in the sample treatment period caused by overlong pretreatment time, long time of the whole detection process, low detection efficiency and the like.
Disclosure of Invention
In order to overcome the defects in the prior art, the embodiment of the invention provides a sample loading detection system and a detection method for blood vitamin D pretreatment, which are used for rapidly detecting vitamin D in a blood sample, reduce the steps of sample pretreatment, and simultaneously can rapidly give a detection report, save the clinical detection time and realize more efficient sample analysis.
The embodiment of the application discloses: a sample loading detection system for blood vitamin D pretreatment, which comprises a sample placing unit to be detected, a sample enrichment sampling needle, a sample elution sample loading needle, a sample elution unit, a single quadrupole rod quality detector and a data processing unit, an inner hole for inserting the sample enrichment sampling needle is arranged in the sample elution loading needle, a sealing piece is arranged at the end part of the sample elution loading needle, the sample elution unit comprises a sample elution solvent and an elution pump which are communicated with the sample elution loading needle, the elution pump is arranged between the sample elution loading needle and the sample elution solvent, filling the sample eluting solvent into the sample eluting and loading needle, connecting the sample eluting and loading needle with a high-voltage power supply, one end of the single quadrupole rod quality detector is connected to the sample eluting and loading needle, and the other end of the single quadrupole rod quality detector is connected to the data processing unit.
Preferably, the surface of the sample enrichment sampling needle is coated with an enrichment material layer.
Preferably, the sample enrichment sampling needle is a solid needle made of a metal material.
Preferably, the material of the sample eluting and loading needle is metal or quartz glass.
Preferably, the diameter of the inner bore of the sample elution sample application needle is larger than the outer diameter of the sample enrichment sample needle.
Preferably, the outer diameter range of the sample enrichment sampling needle is 0.01 mm-1 mm, and the diameter range of an inner hole in the sample elution sampling needle is 0.1 mm-2 mm.
Preferably, the inner diameter of the needle tip of the sample elution loading needle ranges from 0.01mm to 0.1 mm.
Preferably, the sample eluting solvent is methanol.
Preferably, the sealing element is made of a conductive material, the high-voltage power supply is connected to the sealing element, and the voltage range provided by the high-voltage power supply is 500V-5000V.
The application also discloses a detection method for blood vitamin D pretreatment, which comprises the sample loading detection system for blood vitamin D pretreatment, and the monitoring method comprises the following steps:
enriching vitamin D: the sample enrichment sampling needle enters a to-be-detected sample placing unit, and vitamin D in the to-be-detected sample placing unit is adsorbed and enriched;
and (3) elution: filling the sample elution solvent into the sample elution loading needle through the elution pump, sealing the sample elution loading needle by using the sealing piece, extending the sample enrichment sampling needle enriched with vitamin D into the sample elution loading needle through the sealing piece, and eluting the sample solution in the sample elution loading needle;
ionization treatment: the high-voltage power supply transmits the generated high voltage to the sample elution loading needle, and the sample solution in the sample elution loading needle is ionized;
and (3) detection: the sample after ionization processing is directly sent into the single quadrupole mass detector for detection, the detection data of the single quadrupole mass detector are collected through a data acquisition module in a data processing unit and are subjected to data conversion with a data analysis module in the data processing unit, the data are processed and analyzed through the data analysis module in the data processing unit, and finally a detection report is given.
The invention has the following beneficial effects: according to the invention, the vitamin D in the blood can be directly enriched by using the sample enrichment sampling needle, so that the complicated pretreatment step is avoided; after the sample is eluted, ionized and detected, a detection report can be accurately and quickly obtained; the invention has simple design, can realize the rapid detection of blood vitamin D and the high-efficiency analysis, and has industrial utilization value.
In order to make the aforementioned and other objects, features and advantages of the invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a schematic structural diagram in an embodiment of the present invention.
In the figure: 1. a sample enrichment sampling needle; 2. sample elution and loading needle; 3. a sample eluting solvent; 4. an elution pump; 5. a high voltage power supply; 6. a single quadrupole mass detector; 7. a data processing unit; 8. a sample placing unit to be tested; 9. and a seal.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The raw materials, instruments and reagents used in the invention are all commercial products, and can be purchased from the market.
As shown in fig. 1, in a preferred embodiment of the present invention, the blood vitamin D pretreatment sample loading detection system includes a sample placement unit 8 to be detected, a sample enrichment sampling needle 1, a sample elution sampling needle 2, a sample elution unit, a single quadrupole rod quality detector 6 and a data processing unit 7, an inner hole (not shown in the figure) for inserting the sample enrichment sampling needle 1 is disposed in the sample elution sampling needle 2, a sealing member 9 is disposed at an end of the sample elution sampling needle 2, the sample enrichment sampling needle 1 can extend into the inner hole of the sample elution sampling needle 2 through the sealing member 9, the sample elution unit includes a sample elution solvent 3 and an elution pump 4 communicating with the sample elution sampling needle 2, the sample elution solvent 3 is methanol, the elution pump 4 is disposed between the sample elution sampling needle 2 and the sample elution solvent 3, and filling the sample eluting solvent 3 into the sample eluting needle 2; the sample elution sample loading needle 2 is connected with a high-voltage power supply 5, one end of the single quadrupole rod quality detector 6 is connected to the sample elution sample loading needle 2, and the other end of the single quadrupole rod quality detector is connected to the data processing unit 7.
The sample enrichment sampling needle 1 is a solid needle and is coated with an enrichment material layer (not shown in the figure) on the surface thereof, the sample enrichment sampling needle 1 is made of a metal material, the enrichment material layer is conveniently coated on the surface thereof, the material of the sample elution sampling needle 2 is metal, the diameter of an inner hole in the sample elution sampling needle 2 is larger than the outer diameter of the sample enrichment sampling needle 1, the outer diameter of the sample enrichment sampling needle 1 is 0.01mm, the diameter range of the inner hole in the sample elution sampling needle 2 is 0.1mm, and the inner diameter range of the needle point of the sample elution sampling needle 2 is 0.01 mm.
The sealing member 9 is made of a conductive material, the high-voltage power supply 5 is connected to the sealing member 9, and the voltage range provided by the high-voltage power supply 5 is 500V.
In another embodiment, the material of the sample eluting and loading needle 2 is quartz glass.
In another embodiment, the sample enrichment sampling needle 1 has an outer diameter of 1mm, the sample elution sampling needle 2 has an inner bore with a diameter in the range of 2mm, and the sample elution sampling needle 2 has a tip with an inner diameter in the range of 0.1 mm.
In another embodiment, the high voltage power supply 5 provides a voltage in the range of 5000V.
A detection method for blood vitamin D pretreatment comprises the sample loading detection system for blood vitamin D pretreatment, and the monitoring method comprises the following steps:
enriching vitamin D: vertically putting the sample enrichment sampling needle 1 into a horizontally-placed sample placement unit 8 to be detected, and adsorbing and enriching vitamin D of the sample to be detected in the sample placement unit 8 to be detected through an enrichment material on the surface of the sample enrichment sampling needle 1;
and (3) elution: filling the sample elution solvent 3 into the sample elution loading needle 2 through the elution pump 4, sealing the sample elution loading needle by using the sealing piece 9, horizontally extending the sample enrichment sampling needle 1 enriched with vitamin D into the sample elution loading needle 2 through the sealing piece 9, and eluting the sample solution in the sample elution loading needle 3 by using the elution solvent 3 in the sample elution loading needle 2;
ionization treatment: because the sealing member 9 is made of a conductive material and the high voltage power supply 5 is connected to the sealing member 9, the sealing member 9 directly contacts the sample solution in the sample elution loading needle 2, and when the high voltage power supply 5 transmits the generated high voltage to the sample elution loading needle 2 through the sealing member 9, the sample solution in the sample elution loading needle 2 is ionized;
and (3) detection: the ionized sample is directly sent into the single quadrupole mass detector 6 for detection, the detection data of the single quadrupole mass detector 6 are collected through a data acquisition module in a data processing unit 7, data conversion is carried out on the detection data and a data analysis module in the data processing unit 7, the detection data are processed and analyzed through the data analysis module in the data processing unit 7, and finally a detection report is given.
The steps of sample pretreatment in the prior art are reduced, meanwhile, a detection report can be rapidly given, the clinical detection time is saved, and more efficient sample analysis is realized.
The principle and the implementation mode of the invention are explained by applying specific embodiments in the invention, and the description of the embodiments is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (10)
1. A sample loading detection system for blood vitamin D pretreatment is characterized by comprising a sample placing unit to be detected, a sample enrichment sampling needle, a sample elution unit, a single quadrupole rod quality detector and a data processing unit, an inner hole for inserting the sample enrichment sampling needle is arranged in the sample elution loading needle, a sealing piece is arranged at the end part of the sample elution loading needle, the sample elution unit comprises a sample elution solvent and an elution pump which are communicated with the sample elution loading needle, the elution pump is arranged between the sample elution loading needle and the sample elution solvent, filling the sample eluting solvent into the sample eluting and loading needle, connecting the sample eluting and loading needle with a high-voltage power supply, one end of the single quadrupole rod quality detector is connected to the sample eluting and loading needle, and the other end of the single quadrupole rod quality detector is connected to the data processing unit.
2. The loading detection system of claim 1, wherein the sample enrichment sampling needle surface is coated with a layer of enrichment material.
3. The loading detection system of claim 2, wherein the sample enrichment sampling needle is a solid needle made of metal.
4. The system according to claim 1, wherein the sample eluting needle is made of metal or quartz glass.
5. The loading detection system of claim 1, wherein the diameter of the inner bore in the sample eluting loading needle is larger than the outer diameter of the sample enrichment sampling needle.
6. The loading detection system of claim 5, wherein the sample enrichment sampling needle has an outer diameter ranging from 0.01mm to 1mm, and the sample elution loading needle has an inner bore with a diameter ranging from 0.1mm to 2 mm.
7. The loading detection system of claim 1, wherein the sample eluting loading needle tip has an inner diameter in the range of 0.01mm to 0.1 mm.
8. The loading detection system of claim 1, wherein the sample eluting solvent is methanol.
9. The sample loading detection system of claim 1, wherein the sealing member is made of a conductive material, the high voltage power supply is connected to the sealing member, and the voltage supplied by the high voltage power supply ranges from 500V to 5000V.
10. A method for detecting blood vitamin D pretreatment, comprising the blood vitamin D pretreatment sample application detection system according to any one of claims 1 to 9, wherein the monitoring method comprises the following steps:
enriching vitamin D: the sample enrichment sampling needle enters a to-be-detected sample placing unit, and vitamin D in the to-be-detected sample placing unit is adsorbed and enriched;
and (3) elution: filling the sample elution solvent into the sample elution loading needle through the elution pump, sealing the sample elution loading needle by using the sealing piece, extending the sample enrichment sampling needle enriched with vitamin D into the sample elution loading needle through the sealing piece, and eluting the sample solution in the sample elution loading needle;
ionization treatment: the high-voltage power supply transmits the generated high voltage to the sample elution loading needle, and the sample solution in the sample elution loading needle is ionized;
and (3) detection: the sample after ionization processing is directly sent into the single quadrupole mass detector for detection, the detection data of the single quadrupole mass detector are collected through a data acquisition module in a data processing unit and are subjected to data conversion with a data analysis module in the data processing unit, the data are processed and analyzed through the data analysis module in the data processing unit, and finally a detection report is given.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010170581.9A CN111380742A (en) | 2020-03-12 | 2020-03-12 | Sample loading detection system and detection method for blood vitamin D pretreatment |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010170581.9A CN111380742A (en) | 2020-03-12 | 2020-03-12 | Sample loading detection system and detection method for blood vitamin D pretreatment |
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| CN111380742A true CN111380742A (en) | 2020-07-07 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06500403A (en) * | 1991-04-08 | 1994-01-13 | ラ マイナ リミテッド | Biological sample collection test device and collection test method |
| US20110195513A1 (en) * | 2008-02-25 | 2011-08-11 | Waters Technologies Corporation | Methods And Kits For The Determination Of The Presence And Quantity of Vitamin D Analogs In Samples |
| CN102879256A (en) * | 2011-07-11 | 2013-01-16 | 安捷伦科技有限公司 | Apparatus and methods for acquiring analytes from dried biological fluid sample |
| CN106053200A (en) * | 2016-06-29 | 2016-10-26 | 杭州龙鑫科技有限公司 | Urine enrichment device |
| CN205844066U (en) * | 2016-06-07 | 2016-12-28 | 云南中烟工业有限责任公司 | The pretreating device of chemical substance detection in a kind of saliva |
| CN106645374A (en) * | 2016-12-26 | 2017-05-10 | 吉林大学 | Online-internal-extraction electrospray-ionization mass-spectroscopy detection device for rapidly analyzing body fluid samples of human body |
| CN110824076A (en) * | 2019-11-05 | 2020-02-21 | 暨南大学 | Solid phase micro-extraction swab device and method |
-
2020
- 2020-03-12 CN CN202010170581.9A patent/CN111380742A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06500403A (en) * | 1991-04-08 | 1994-01-13 | ラ マイナ リミテッド | Biological sample collection test device and collection test method |
| US20110195513A1 (en) * | 2008-02-25 | 2011-08-11 | Waters Technologies Corporation | Methods And Kits For The Determination Of The Presence And Quantity of Vitamin D Analogs In Samples |
| CN102879256A (en) * | 2011-07-11 | 2013-01-16 | 安捷伦科技有限公司 | Apparatus and methods for acquiring analytes from dried biological fluid sample |
| CN205844066U (en) * | 2016-06-07 | 2016-12-28 | 云南中烟工业有限责任公司 | The pretreating device of chemical substance detection in a kind of saliva |
| CN106053200A (en) * | 2016-06-29 | 2016-10-26 | 杭州龙鑫科技有限公司 | Urine enrichment device |
| CN106645374A (en) * | 2016-12-26 | 2017-05-10 | 吉林大学 | Online-internal-extraction electrospray-ionization mass-spectroscopy detection device for rapidly analyzing body fluid samples of human body |
| CN110824076A (en) * | 2019-11-05 | 2020-02-21 | 暨南大学 | Solid phase micro-extraction swab device and method |
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Application publication date: 20200707 |