CN111363667A - Cell extraction and filtration device and filtration method thereof - Google Patents
Cell extraction and filtration device and filtration method thereof Download PDFInfo
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- CN111363667A CN111363667A CN202010308582.5A CN202010308582A CN111363667A CN 111363667 A CN111363667 A CN 111363667A CN 202010308582 A CN202010308582 A CN 202010308582A CN 111363667 A CN111363667 A CN 111363667A
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- 238000001914 filtration Methods 0.000 title claims abstract description 124
- 238000000605 extraction Methods 0.000 title claims abstract description 58
- 239000007788 liquid Substances 0.000 claims abstract description 63
- 238000003756 stirring Methods 0.000 claims description 90
- 239000007924 injection Substances 0.000 claims description 38
- 238000002347 injection Methods 0.000 claims description 38
- 238000002156 mixing Methods 0.000 claims description 28
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 23
- 239000002699 waste material Substances 0.000 claims description 18
- 239000011259 mixed solution Substances 0.000 claims description 15
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 13
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 13
- 241001330002 Bambuseae Species 0.000 claims description 13
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 13
- 239000011425 bamboo Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 13
- 239000004744 fabric Substances 0.000 claims description 8
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 2
- 210000001519 tissue Anatomy 0.000 description 65
- 239000000203 mixture Substances 0.000 description 11
- 238000003780 insertion Methods 0.000 description 7
- 230000037431 insertion Effects 0.000 description 7
- 238000009434 installation Methods 0.000 description 5
- 238000003915 air pollution Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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Abstract
The invention relates to the technical field of cell extraction and filtration, in particular to a cell extraction and filtration device which comprises a filter cartridge for filtering mixed liquid of cell tissues, wherein a filter opening is formed in the filter cartridge, the cell extraction and filtration device also comprises a filter tank body, a filter cover and a filter screen, the filter tank body is connected with a negative pressure pipe and an extraction pipe, the filter cover is arranged on the filter tank body, the filter cartridge and the filter screen are both connected with the filter cover, the filter cover is provided with a filter opening, the opening of the filter cartridge is communicated with the filter opening, the filter cartridge is positioned in the filter screen, the filter screen is positioned in the filter tank body, a gap is reserved between the filter screen and the filter tank body, the bottom of the filter screen is provided with a discharge hole, the. The purity of the extracted cell tissue is higher.
Description
Technical Field
The invention relates to the technical field of cell extraction and filtration, in particular to a cell extraction and filtration device and a filtration method thereof.
Background
Currently, in biomedical research, in vitro research is often performed using cells as a model. In the research process, filtration and extraction of cells are often involved. Particularly, when some instruments are used for detection, in order to ensure the detection accuracy and the practical safety of the instruments, the cells to be detected need to be filtered and extracted, and large tissues (such as connective tissues) in the cell tissue mixed liquid need to be filtered.
Chinese patent No. CN201924018U discloses a cell filtration device, which comprises a filter cartridge, wherein the open end of the filter cartridge is provided with a brim, and the other end of the filter cartridge is conical; the filter cylinder consists of an annular brim inner ring, a support rod fixedly connected to the brim inner ring and a filter membrane glued on the support rod, wherein the bottom of the filter membrane is conical.
The above prior art solutions have the following drawbacks: when the cell filtering device filters the cell tissue mixed liquid, the cell tissue mixed liquid is directly exposed in the air and is easy to be polluted, so that the purity of the extracted cell tissue is low.
Disclosure of Invention
In view of the shortcomings of the prior art, it is an object of the present invention to provide a cell extraction and filtration device, which can extract cell tissue with high purity.
The above object of the present invention is achieved by the following technical solutions:
the utility model provides a cell extraction filter equipment, including the cartridge filter that is used for filtering cell tissue mixed liquid, be equipped with the filtration opening on the cartridge filter, still including filtering the jar body, filter lid and filter screen, be connected with negative pressure tube and extraction tube on filtering the jar body, filter the lid setting on filtering the jar body, cartridge filter and filter screen all link to each other with filtering the lid, filter and cover and be equipped with the filtration mouth, the nozzle and the filtration mouth intercommunication of cartridge filter, the cartridge filter is in the filter screen, it is internal that the filter screen is in filtering the jar, leave the space between filter screen and the filtering jar body, the filter screen bottom is equipped with the discharge opening, discharge opening intercommunication extraction tube, space intercommunication negative pressure tube between.
Through adopting above-mentioned technical scheme, when drawing cell tissue mixed liquid, earlier to filtering jar internal injection normal saline, the cell tissue mixed liquid that will draw is poured into the cartridge filter again, the cartridge filter is to cell tissue mixed liquid, filter the bold tissue, cell tissue mixed liquid after the filtration is internal at filtering jar, take out waste liquid composition and normal saline through the negative pressure pipe, the rethread extraction tube intercepts the filter screen and removes the cell extraction of waste liquid composition, the filtering process is internal at filtering jar, cell tissue liquid is difficult to by air pollution, cell tissue purity after the extraction is higher.
The present invention in a preferred example may be further configured to: the filtration lid can be dismantled with the filtration jar body and be connected, and the filter screen can be dismantled with the filtration lid and be connected.
Through adopting above-mentioned technical scheme, after filtering, the user can dismantle the filter cover from filtering the jar body, again with the filter screen from filtering the cover dismantlement to wash the filter screen and the filter jar body alone, so that guarantee this filter equipment's clean degree.
The present invention in a preferred example may be further configured to: the filter cover is provided with a first connecting ring and a second connecting ring, the first connecting ring is positioned in the second connecting ring, the filter screen is in threaded connection with the first connecting ring, and the filter tank body is in threaded connection with the second connecting ring.
Through adopting above-mentioned technical scheme, twist and move the filtration jar body and can realize filtering the installation and the dismantlement between the jar body and the filter lid, make the installation of filtering the jar body and dismantle the process swiftly, convenient, twist and move the filter screen and can realize the installation and the dismantlement between filter screen and the filter lid, make the installation of filter screen and dismantle the process swiftly, convenient, bring the convenience for the user.
The present invention in a preferred example may be further configured to: the filter screen includes rack and screen cloth, and the rack links to each other with filtering the lid, and the screen cloth setting is on the rack, and the rack bottom is the toper, and the discharge opening setting is on the rack bottom, and the jar body bottom of filtering sets up to the round platform shape, and negative pressure pipe and extraction tube all link to each other with the horizontal segment that filters jar body bottom.
Through adopting above-mentioned technical scheme, set up the rack bottom into the toper, the extraction of the cell of being convenient for, will filter jar body bottom and set up to the round platform shape, the extraction of the waste liquid composition of being convenient for and normal saline.
The present invention in a preferred example may be further configured to: the extraction tube links to each other bottom the jar body with filtering through the grafting pipe, and the grafting pipe is the round platform shape, and the big aperture end of grafting pipe links to each other with filtering jar body, and the small aperture end of grafting pipe links to each other with the extraction tube, and the rack bottom is equipped with the connecting pipe of intercommunication discharge opening, and the connecting pipe is in the grafting intraductal, connecting pipe and extraction tube intercommunication.
Through adopting above-mentioned technical scheme, it is intraductal that the connecting pipe is in the grafting, conveniently fixes a position the filter screen, has reduced the filter screen and has taken place the possibility of skew in the internal position of filter tank, and in cell accessible discharge opening and connecting pipe directly entered into the extraction tube, makes things convenient for drawing of cell.
The present invention in a preferred example may be further configured to: still include the stirring subassembly, the stirring subassembly includes the (mixing) shaft, stirring vane and stirring cap, the (mixing) shaft rotates with cartridge filter bottom to be connected and runs through a section of thick bamboo bottom, stirring vane sets up on the (mixing) shaft and is in the cartridge filter below, the stirring cap sets up on the cartridge filter and shelters from the nozzle of cartridge filter, be equipped with one end open-ended injection section of thick bamboo on the stirring cap, the injection section of thick bamboo runs through the stirring cap, the injection bobbin base portion can be dismantled with the (mixing) shaft and be connected, the one end that the injection section of thick bamboo lateral.
Through adopting above-mentioned technical scheme, when filtering cell tissue mixed liquid, inject into the cell tissue mixed liquid and pour into a section of thick bamboo into, cell tissue mixed liquid passes through the filling hole and discharges and filter in entering into the cartridge filter, cell tissue mixed liquid after the filtration enters into jar internal, rotate the stirring cap, the stirring cap passes through the (mixing) shaft and drives stirring vane and rotate, stirring vane is more even with the cell tissue mixed liquid stirring after the filtration, be favorable to the purification effect of cell, and the stirring cap shelters from the nozzle of cartridge filter, further improve the seal of this device, cell tissue mixed liquid has further been reduced and has received air pollution's possibility.
The present invention in a preferred example may be further configured to: and the stirring blade is provided with a notch.
Through adopting above-mentioned technical scheme, on the one hand, can alleviate stirring vane's weight, the stirring of edge stirring vane, another is sent out, at the in-process of stirring cell equipment mixed liquid, and cell tissue mixed liquid accessible breach circulation helps the flow of cell tissue mixed liquid.
The present invention in a preferred example may be further configured to: the detachable rotation is connected between (mixing) shaft and the cartridge filter, the elastic clamping pipe that the filter cartridge bottom was equipped with mounting hole and intercommunication mounting hole is equipped with annular protrusion on the intraductal lateral wall of elastic clamping, still is equipped with the joint mouth that the elastic clamping pipe of being convenient for takes place deformation on the elastic clamping pipe, is equipped with the annular groove on the (mixing) shaft, and the (mixing) shaft runs through elastic clamping pipe and mounting hole, and annular protrusion is in the annular groove, annular protrusion and annular groove normal running fit.
Through adopting above-mentioned technical scheme, if cartridge filter or (mixing) shaft are damaged, then can dismantle the change alone with the part of damage, and needn't all change cartridge filter and (mixing) shaft, save the replacement cost.
The present invention in a preferred example may be further configured to: the stirring shaft is provided with a clamping groove, the bottom of the injection cylinder is provided with a clamping hole, a clamping block is arranged in the clamping hole, one end of the stirring shaft connected with the injection cylinder is positioned in the clamping hole, and the clamping block is inserted in the clamping groove.
Through adopting above-mentioned technical scheme, when needs rotate the (mixing) shaft, with the joint hole cover on the (mixing) shaft, and make the joint piece be in the joint inslot, rotate the stirring cap, the stirring cap drives the (mixing) shaft through pouring into a section of thick bamboo and rotates, thereby realize drive (mixing) shaft pivoted purpose, when need not rotate the (mixing) shaft, pulling the stirring cap, make and pour into a section of thick bamboo and (mixing) shaft separation, thereby no longer continue to rotate the (mixing) shaft, and pour into being connected between a section of thick bamboo and the (mixing) shaft, dismantlement process convenient and.
Another object of the present invention is to provide a filtration method of a cell extraction filtration apparatus, comprising the steps of:
s1: injecting physiological saline into the filtering tank body in advance:
s2: injecting the mixed liquid of cell tissues to be extracted into a filter tank body, and filtering the mixed liquid of cell tissues by a filter cylinder;
s3: stirring the mixed solution of the cell tissues and the normal saline together by a stirring component;
s4: connecting a negative pressure device on the negative pressure pipe, and using the negative pressure device to pump out the mixed liquid of the waste liquid components and the normal saline in the filtering tank body;
s5: extracting the filtered cell tissue from which the waste liquid components are removed.
Through adopting above-mentioned technical scheme, when filtering cell tissue mixed liquid, earlier to filtering internal injection normal saline of jar, it is internal to inject the cell tissue mixed liquid that will draw into filtering jar again, the cartridge filter filters cell tissue mixed liquid, the tissue mixed liquid and the normal saline stirring after the rethread stirring subassembly is to the filtration, mix cell tissue mixed liquid and normal saline stirring, take out the mixed liquid of waste liquid composition and normal saline in the jar body of filtering through negative pressure device and negative pressure pipe, the cell tissue who will remove waste liquid composition at last draws, reach the purpose that the cell filtered was drawed.
In summary, the invention includes at least one of the following beneficial technical effects:
1. when extracting the cell tissue mixed solution, firstly injecting normal saline into the filter tank body, then injecting the cell tissue mixed solution to be extracted into the filter cylinder, filtering the cell tissue mixed solution by the filter cylinder, filtering large-block tissues, pumping out waste liquid components and normal saline through the negative pressure pipe after filtering the cell tissue mixed solution in the filter tank body, intercepting the filter screen through the extraction pipe, and extracting cells for removing the waste liquid components, wherein the filtering process is performed in the filter tank body, the cell tissue solution is not easily polluted by air, and the purity of the extracted cell tissue is higher;
2. after the filtration is finished, a user can detach the filter cover from the filter tank body and then detach the filter screen from the filter cover so as to independently clean the filter screen and the filter tank body and ensure the cleanliness of the filter device;
3. set up the rack bottom into the toper, the extraction of the cell of being convenient for, will filter jar body bottom and set up to the round platform shape, the extraction of the waste liquid composition of being convenient for and normal saline.
Drawings
Fig. 1 is a schematic structural diagram for showing a positional relationship between a liquid level line and a filtration tank in embodiment 1.
FIG. 2 is a schematic structural view for showing a positional relationship between a filter screen and a filter tank in embodiment 1;
FIG. 3 is a schematic view of the structure for showing the positional relationship between the negative pressure tube and the extraction tube in example 1;
FIG. 4 is an enlarged view of the portion A of FIG. 2 illustrating the connection between the second connection ring and the filter tank;
FIG. 5 is a schematic view of a filter screen according to embodiment 1;
fig. 6 is a schematic structural diagram for showing the positional relationship between the elastic snap-in pipe and the filter cartridge in embodiment 1;
FIG. 7 is a schematic view of the structure for showing the positional relationship between the center hole and the stirring shaft in example 1;
FIG. 8 is a schematic structural view of FIG. 2 showing the positional relationship between the annular projection and the annular groove;
fig. 9 is a schematic structural diagram for showing a positional relationship between the snap-in block and the snap-in hole in embodiment 1.
In the drawings, 1, a filter cartridge; 2. filtering the tank body; 3. a filter cover; 4. a filter screen; 5. a liquid level line; 6. a support leg; 7. a rib plate; 8. a negative pressure tube; 9. an extraction tube; 10. inserting a pipe; 11. a second connection ring; 12. a net frame; 13. screen cloth; 14. a first connecting ring; 15. a first limit ring; 16. a connecting pipe; 17. a filtration port; 18. circular filter holes; 19. a stirring shaft; 20. a stirring blade; 21. a stirring cap; 22. an elastic clamping pipe; 23. an annular projection; 24. a card interface; 25. an annular groove; 26. a second stop collar; 27. an injection cylinder; 28. a card slot; 29. a clamping hole; 30. a clamping block; 31. an injection hole; 32. a central bore.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1: referring to fig. 1 and 2, a cell extraction and filtration apparatus disclosed in the present invention includes a filter cartridge 1, a filter tank 2, a filter cover 3, a filter screen 4, and a stirring assembly.
Referring to fig. 1 and 2, a liquid level line 5 is arranged on the filtering tank body 2, the filtering tank body 2 is a transparent plastic tank body, a support leg 6 is connected to the filtering tank body 2 so as to support the filtering tank body 2, the filtering tank body 2 can stand on a plane, and a rib plate 7 is connected between the support leg 6 and the filtering tank body 2 so as to improve the stability between the support leg 6 and the filtering tank body 2, in the embodiment, the filtering tank body 2, the support leg 6 and the rib plate 7 are integrally formed; referring to fig. 2 and 3, the bottom of the filtering tank body 2 is set to be in a truncated cone shape, the horizontal part of the bottom of the filtering tank body 2 is connected with a negative pressure pipe 8 and an extraction pipe 9, the extraction pipe 9 is connected with the bottom of the filtering tank body 2 through an insertion pipe 10, the insertion pipe 10 is in a truncated cone shape, the caliber of one end of the insertion pipe 10 is large, the caliber of the other end of the insertion pipe 10 is small, the large-caliber end of the insertion pipe 10 is connected with the filtering tank body 2, the small-caliber end of the insertion pipe 10 is connected with the extraction pipe 9, and the central line of the extraction pipe 9, the central line of the insertion pipe.
Referring to fig. 2 and 4, the filter cover 3 is arranged on the filter tank body 2 and detachably connected with the filter tank body 2, the filter cover 3 is connected with a second connecting ring 11, the second connecting ring 11 is detachably connected with the filter tank body 2 through threads, inner threads are arranged on the inner side wall of the second connecting ring 11, outer threads matched with the inner threads on the second connecting ring 11 are arranged on the outer side wall of the filter tank body 2, the inner threads are in threaded connection with the outer threads, the second connecting ring 11 and the filter cover 3 are integrally formed, and the central line of the second connecting ring 11, the central line of the filter cover 3 and the central line of the filter tank body 2 coincide.
Referring to fig. 4 and 5, the filter screen 4 is detachably connected with the filter cover 3, the filter screen 4 includes a net frame 12 and a net cloth 13, the bottom of the net frame 12 is conical, the net cloth 13 is disposed on the net frame 12, the filter cover 3 is connected with a first connecting ring 14, the first connecting ring 14 is disposed in a second connecting ring 11, the center line of the first connecting ring 14 coincides with the center line of the second connecting ring 11, the net frame 12 of the filter screen 4 is detachably connected with the first connecting ring 14 through a thread, an inner thread is disposed on the inner side wall of the first connecting ring 14, an outer thread matched with the inner thread of the first connecting ring 14 is disposed on the net frame 12, the inner thread is screwed with the outer thread, the net frame 12 is further connected with a first limit ring 15, the first connecting ring 14 abuts, so as to carry on spacingly to first connecting ring 14, in this embodiment, the screen cloth 13 bonds on the rack 12 inside wall through glue, and first connecting ring 14 sets up with filter lid 3 integrated into one piece.
Referring to fig. 2 and 5, filter screen 4 is in filtering jar body 2, leave the space between filter screen 4 and the filtering jar body 2, and filter screen 4 and the space intercommunication negative pressure pipe 8 between the filtering jar body 2, net rack 12 bottom of filter screen 4 is equipped with the discharge opening, net rack 12 bottom still is connected with the connecting pipe 16 of intercommunication discharge opening, connecting pipe 16 and net rack 12 bottom integrated into one piece set up, connecting pipe 16 is in the pipe 10 of pegging graft, connecting pipe 16 and extraction tube 9 intercommunication, and the central line of connecting pipe 16 and the coincidence of the central line of extraction tube 9.
Referring to fig. 2 and 6, the filter cartridge 1 is used for filtering mixed liquid of cell tissues, the filter cartridge 1 is connected with the filter cover 3, the edge of the cartridge opening of the filter cartridge 1 is arranged in an eversion way, and cartridge filter 1 and filter lid 3 integrated into one piece set up, be equipped with the filtration mouth on the filter lid 3, the nozzle and the filtration mouth intercommunication of cartridge filter 1, cartridge filter 1 is in filter screen 4, filter lid 3's central line, the coincidence of central line and cartridge filter 1's central line, be equipped with on the cartridge filter 1 and filter opening 17 and circular filtration hole 18, it is L shape to filter opening 17, the vertical part of filtering opening 17 is separately established on cartridge filter 1 lateral wall, the horizontal part of filtering opening 17 is separately established in cartridge filter 1 bottom, circular filtration hole 18 is seted up in cartridge filter 1 bottom, filter opening 17 and circular filtration hole 18 cooperation use, so that filter the mixed liquid of cell tissue, organize the interception with the bold.
Referring to fig. 2, the stirring assembly includes a stirring shaft 19, a stirring blade 20, and a stirring cap 21.
Referring to fig. 2 and 7, the stirring shaft 19 is provided with a central hole 32 along the length direction thereof so as to reduce the weight of the stirring shaft 19, the stirring shaft 19 is detachably and rotatably connected with the bottom of the filter cartridge 1 and penetrates through the bottom of the filter cartridge 1, referring to fig. 6 and 8, the bottom of the filter cartridge 1 is provided with mounting holes and an elastic clamping tube 22 communicated with the mounting holes, the elastic clamping tube 22 is integrally formed with the filter cartridge 1, an annular protrusion 23 is connected to the inner side wall of one end of the elastic clamping tube 22 away from the filter cartridge 1, the annular protrusion 23 is integrally formed with the elastic clamping tube 22, the filter cartridge 1, the elastic clamping tube 22 and the annular protrusion 23 are made of PE plastic material, the elastic clamping tube 22 is further provided with three clamping ports 24 facilitating deformation of the elastic clamping tube 22, the three clamping ports 24 are arranged in an annular array with the center line of the elastic clamping tube 22 as the center, and the clamping, be equipped with annular groove 25 on the (mixing) shaft 19, the (mixing) shaft 19 runs through elasticity joint pipe 22 and mounting hole, annular bulge 23 is in annular groove 25, annular bulge 23 and annular groove 25 normal running fit, and be connected with second spacing ring 26 on the (mixing) shaft 19, elasticity joint pipe 22 contradicts second spacing ring 26 from the one end of cartridge filter 1 to it is spacing to carry out elasticity joint pipe 22, when installation (mixing) shaft 19, make in annular bulge 23 smooth entering annular groove 25.
Referring to fig. 2, the stirring blade 20 is disposed on the stirring shaft 19 and below the filter cartridge 1, and the stirring blade 20 is disposed in the filter screen 4, and the stirring blade 20 is provided with a notch, so that on one hand, the weight of the stirring blade 20 can be reduced, and on the other hand, the flow of the cell tissue mixed liquid is facilitated.
Referring to fig. 2 and 9, the stirring cap 21 is erected on the filter cartridge 1 and shields the cartridge opening of the filter cartridge 1 and the filter opening of the filter cover 3, the stirring cap 21 is connected with an injection cylinder 27 with one open end, the injection cylinder 27 and the stirring cap 21 are integrally formed, the injection cylinder 27 penetrates through the stirring cap 21, the bottom of the injection cylinder 27 is located in the filter cartridge 1 and detachably connected with the stirring shaft 19, the stirring shaft 19 is provided with a clamping groove 28, the bottom of the injection cylinder 27 is provided with a clamping hole 29, a clamping block 30 is arranged in the clamping hole 29, one end of the stirring shaft 19, which is connected with the injection cylinder 27, is located in the clamping hole 29, the clamping block 30 is inserted in the clamping groove 28, and one end, which is close to the bottom of the injection cylinder 27, of the side wall of the injection cylinder.
The implementation principle of the embodiment is as follows: when extracting the cell tissue mixed solution, two thirds of normal saline is injected into the tank body in advance, then the stirring cap 21 is put on the filter cartridge 1, the clamping block 30 is inserted into the clamping groove 28, the cell tissue mixed solution to be extracted is injected into the injection cylinder 27 through the injector, the cell tissue mixed solution is discharged from the injection cylinder 27 through the injection hole 31 and then enters the filter cartridge 1, the filter cartridge 1 filters the cell tissue mixed solution through the filtering through hole 17 and the circular filtering hole 18, the large block tissue in the cell tissue mixed solution is filtered, the filtered cell tissue mixed solution is in the filter tank body 2, the stirring cap 21 is rotated, the stirring cap 21 drives the injection cylinder 27 to rotate, the injection cylinder 27 drives the stirring shaft 19 to rotate through the matching of the clamping block 30 and the clamping groove 28, the stirring shaft 19 drives the stirring blade 20 to rotate in the rotating process, so as to uniformly stir the filtered cell tissue mixed solution and the normal saline, connect negative pressure equipment on negative pressure pipe 8, in this embodiment, negative pressure equipment is the negative pressure machine, uses the negative pressure machine to take out the mixed liquid of waste liquid composition and normal saline in the jar body 2 of will filtering, takes out the cell tissue extraction of waste liquid composition in will filtering net 4 through extraction tube 9 at last, and the filtering process goes on in filtering jar body 2, and cell tissue liquid is difficult to by air pollution, and the cell tissue purity after the extraction is higher, has guaranteed the aseptic nature of cell tissue.
Example 2: the invention discloses a filtering method of a cell extraction and filtration device, which comprises the following steps:
s1: physiological saline is injected into the filtering tank body 2 in advance:
s2: injecting the mixed liquid of cell tissue to be extracted into the filtering tank body 2 through the injection cylinder 27, and filtering the mixed liquid of cell tissue by the filter cylinder 1;
s3: the cell tissue mixed solution and the normal saline are stirred together by the stirring component, the stirring cap 21 is rotated, the stirring cap 21 drives the injection cylinder 27 to rotate, the injection cylinder 27 drives the stirring shaft 19 to rotate in the rotating process, the stirring shaft 19 drives the stirring blades 20 to rotate, and the cell tissue mixed solution and the normal saline are uniformly stirred by the stirring blades 20;
s4: a negative pressure device is connected on the negative pressure pipe 8, and after the mixture is fully stirred, the mixture of the waste liquid components and the normal saline in the filtering tank body 2 is pumped out by the negative pressure device;
s5: the cells after filtration and removal of the waste components were extracted by syringe.
The implementation process comprises the following steps: when filtering the cell tissue mixed liquid, firstly injecting two thirds of normal saline into the filtering tank body 2, covering the stirring cap 21 on the filter cartridge 1, inserting the clamping block 30 on the injection cylinder 27 into the clamping groove 28 on the stirring shaft 19, injecting the cell tissue mixed liquid to be extracted into the injection cylinder 27 through the injector, allowing the cell tissue mixed liquid to enter the filter cartridge 1 through the injection hole 31, filtering the cell tissue mixed liquid by the filter cartridge 1, rotating the stirring cap 21, driving the injection cylinder 27 to rotate by the stirring cap 21, driving the stirring shaft 19 to rotate during the rotation of the injection cylinder 27, driving the stirring blade 20 to rotate by the stirring shaft 19, uniformly stirring the cell tissue mixed liquid and the normal saline by the stirring blade 20, and extracting the mixed liquid of the waste liquid component and the normal saline in the filtering tank body 2 through the negative pressure device and the negative pressure pipe 8, in the embodiment, the negative pressure device is a negative pressure machine, finally, the cells without the waste liquid components are extracted by the injector to achieve the purpose of cell filtration and extraction, the purity of the extracted cells is higher, and the cells are filtered in the filtering tank body 2, so that the sterility of the cell tissues is ensured, and the possibility of the cell tissues being polluted is reduced.
The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the structure, shape and principle of the invention are covered by the protection scope of the invention.
Claims (10)
1. The utility model provides a cell extraction filter equipment, is equipped with on cartridge filter (1) and filters opening (17), its characterized in that including cartridge filter (1) that is used for filtering cell tissue mixed liquid: still including filtering jar body (2), cross filter lid (3) and filter screen (4), be connected with negative pressure pipe (8) and extraction tube (9) on the filter jar body (2), it sets up on the filter jar body (2) to cross filter lid (3), cartridge filter (1) and filter screen (4) all link to each other with cross filter lid (3), be equipped with the filtration mouth on crossing filter lid (3), the nozzle and the filtration mouth intercommunication of cartridge filter (1), cartridge filter (1) is in filter screen (4), filter screen (4) are in the filter jar body (2), leave the space between filter screen (4) and the filter jar body (2), filter screen (4) bottom is equipped with the discharge opening, discharge opening intercommunication extraction tube (9), space intercommunication negative pressure pipe (8) between filter screen (4) and the filter jar body (2).
2. The cell extraction filter assembly of claim 1, wherein: the filtering cover (3) is detachably connected with the filtering tank body (2), and the filter screen (4) is detachably connected with the filtering cover (3).
3. The cell extraction filter assembly of claim 2, wherein: the filter cover (3) is provided with a first connecting ring (14) and a second connecting ring (11), the first connecting ring (14) is positioned in the second connecting ring (11), the filter screen (4) is in threaded connection with the first connecting ring (14), and the filter tank body (2) is in threaded connection with the second connecting ring (11).
4. The cell extraction filter assembly of claim 1, wherein: the filter screen (4) includes rack (12) and screen cloth (13), and rack (12) link to each other with filtering lid (3), and screen cloth (13) set up on rack (12), and rack (12) bottom is the toper, and the discharge opening setting is on rack (12) bottom, and the filtering tank body (2) bottom sets up to the round platform shape, and negative pressure pipe (8) and extraction tube (9) all link to each other with the horizontal segment that filters tank body (2) bottom.
5. The cell extraction filter assembly of claim 4, wherein: the utility model discloses a filter tank body, including extraction pipe (9), the big aperture end of grafting pipe (10) links to each other with the filtration jar body (2), and the extraction pipe (9) are connected through grafting pipe (10) and filtration jar body (2) bottom, and grafting pipe (10) are round platform shape, and the big aperture end of grafting pipe (10) links to each other with the filtration jar body (2), and the small aperture end of grafting pipe (10) links to each other with extraction pipe (9), and rack (12) bottom is equipped with connecting pipe (16) of intercommunication discharge opening, and connecting pipe (16) are in grafting pipe (10), and connecting pipe (16) and extraction pipe.
6. The cell extraction filter assembly of claim 1, wherein: still include the stirring subassembly, the stirring subassembly includes (mixing) shaft (19), stirring vane (20) and stirring cap (21), stirring shaft (19) rotate with cartridge filter (1) bottom and are connected and run through cartridge filter (1) bottom, stirring vane (20) set up on (mixing) shaft (19) and are in cartridge filter (1) below, stirring cap (21) are set up on cartridge filter (1) and are sheltered from the nozzle of cartridge filter (1), be equipped with one end open-ended injection section of thick bamboo (27) on stirring cap (21), injection section of thick bamboo (27) run through stirring cap (21), injection section of thick bamboo (27) bottom can be dismantled with (mixing) shaft (19) and be connected, the one end that injection section of thick bamboo (27) lateral wall is close to injection section of thick bamboo (27) bottom is equipped with filling hole.
7. The cell extraction filter assembly of claim 6, wherein: the stirring blade (20) is provided with a notch.
8. The cell extraction filter assembly of claim 6, wherein: detachable rotates between (mixing) shaft (19) and the cartridge filter (1) to be connected, cartridge filter (1) bottom is equipped with elasticity joint pipe (22) of mounting hole and intercommunication mounting hole, be equipped with annular protrusion (23) on elasticity joint pipe (22) inside wall, still be equipped with joint mouth (24) that the elasticity joint pipe (22) of being convenient for take place deformation on elasticity joint pipe (22), be equipped with annular groove (25) on (mixing) shaft (19), elastic joint pipe (22) and mounting hole are run through in (mixing) shaft (19), annular protrusion (23) are in annular groove (25), annular protrusion (23) and annular groove (25) normal running fit.
9. The cell extraction filter assembly of claim 6, wherein: the stirring shaft (19) is provided with a clamping groove (28), the bottom of the injection barrel (27) is provided with a clamping hole (29), a clamping block (30) is arranged in the clamping hole (29), one end, connected with the injection barrel (27), of the stirring shaft (19) is located in the clamping hole (29), and the clamping block (30) is inserted into the clamping groove (28).
10. A method of filtering the cell extraction filter device according to any one of claims 1 to 9, wherein: it comprises the following steps:
s1: physiological saline is injected into the filtering tank body (2) in advance:
s2: injecting cell tissue mixed liquor to be extracted into a filtering tank body (2), and filtering the cell tissue mixed liquor by a filter cylinder (1);
s3: stirring the mixed solution of the cell tissues and the normal saline together by a stirring component;
s4: a negative pressure device is connected on the negative pressure pipe (8), and the mixed liquid of the waste liquid components and the normal saline in the filtering tank body (2) is extracted and filtered by the negative pressure device;
s5: extracting the filtered cell tissue from which the waste liquid components are removed.
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| CN202010308582.5A CN111363667A (en) | 2020-04-18 | 2020-04-18 | Cell extraction and filtration device and filtration method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010308582.5A CN111363667A (en) | 2020-04-18 | 2020-04-18 | Cell extraction and filtration device and filtration method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117889485A (en) * | 2024-03-14 | 2024-04-16 | 山西低碳环保产业集团有限公司 | Assembled energy-saving heating and ventilation device and ventilation method thereof |
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Application publication date: 20200703 |