CN111359012A - Artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation and preparation method thereof - Google Patents
Artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation and preparation method thereof Download PDFInfo
- Publication number
- CN111359012A CN111359012A CN202010330989.8A CN202010330989A CN111359012A CN 111359012 A CN111359012 A CN 111359012A CN 202010330989 A CN202010330989 A CN 202010330989A CN 111359012 A CN111359012 A CN 111359012A
- Authority
- CN
- China
- Prior art keywords
- gelatin
- spinning
- ctgf
- kgn
- tendon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/222—Gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0092—Electro-spinning characterised by the electro-spinning apparatus characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F9/00—Artificial filaments or the like of other substances; Manufacture thereof; Apparatus specially adapted for the manufacture of carbon filaments
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/10—Materials or treatment for tissue regeneration for reconstruction of tendons or ligaments
Abstract
The invention belongs to the technical field of surgical patches and provides an artificial rotator cuff patch capable of inducing a tendon-bone gradient structure to form and a preparation method thereof. The preparation method of the artificial rotator cuff patch capable of inducing the tendon-bone gradient structure to form is simple, the obtained three-section artificial rotator cuff patch can promote the tendon-fibrocartilage-bone gradient structure to form, and the three-section artificial rotator cuff patch has good mechanical property, biocompatibility and clinical application prospect.
Description
Technical Field
The invention belongs to the technical field of surgical patches, and particularly relates to an artificial rotator cuff patch capable of inducing a tendon-bone gradient structure to form and a preparation method thereof.
Background
The rotator cuff is an important structure for supporting and stabilizing the glenohumeral joint, and plays an important role in maintaining the sealing function of a shoulder joint cavity, maintaining synovial fluid nutrition articular cartilage and preventing secondary osteoarthritis. Rotator cuff tears are a common disease of the shoulder joint, with increasing incidence with age. Rotator cuff tears are common causes of shoulder joint pain, reduced mobility and reduced function, accounting for about 60% of shoulder lesions. For the patients with ineffective conservative treatment and the patients with large-area rotator cuff injury, the torn rotator cuff is usually repaired by an operation so as to relieve pain and recover the function of the rotator cuff, but the effect after the operation is not ideal, the failure revision rate is 20-70 percent, which is related to the factors of rotator cuff tearing range, tendon contracture, fat infiltration, improper rehabilitation exercise after the operation and the like of the patients, but the tendon-bone interface is not easy to heal after the operation.
The tendon is a weak point in the rotator cuff repair process, especially when the defect is large, the traction in the operation and the attempt to fix the large torn rotator cuff tissue to the original attachment point before the injury are difficult, and at the moment, the rotator cuff structure reinforcement technology is needed to solve the problem of insufficient humeral head coverage when the large rotator cuff is torn, so that the mechanical property of the rotator cuff tissue after the repair operation is ensured, and the stability and the function of the rotator cuff are maintained. The rotator cuff patch reinforcing technology is a commonly used rotator cuff structure reinforcing technology and is particularly suitable for patients with large rotator cuff tear and chronic rotator cuff injury with tendon retraction. With the continuous improvement of tissue engineering technology, the research and development and application of rotator cuff patches enter a new stage, and a plurality of patch materials show good performance, but have certain defects.
Common rotator cuff patch materials include patches of synthetic material and patches of biomaterial. The synthetic material rotator cuff patch comprises a degradable type and a non-degradable type, and the main component is a polymer. Common non-degradable patch synthetic materials comprise polypropylene, polyester, polytetrafluoroethylene, nylon and the like, and the patch has good tensile resistance and provides stable mechanical guarantee for healing of host tissues. The degradable patch synthetic material comprises levorotatory polylactic acid, lactic glycolic acid, polycaprolactone, a copolymer of polypropylene glycol and the like, can be degraded by tissues, and can provide mechanical strength to a certain degree, but degradation products may have certain cytotoxicity. At present, the biocompatibility of the synthetic material rotator cuff patch is poor, and the risk of foreign body rejection reaction of an organism exists after operation, so that the clinical application of the patch is limited to a certain extent.
The biomaterial patch is mostly derived from tissue graft materials, and can be roughly classified into autologous tissue transplantation, allogeneic tissue transplantation, decellularized tissue transplantation, and the like according to the source thereof. The autograft material is mainly derived from fascia lata, tendon of long head of biceps brachii and the like, has the greatest advantage of good biocompatibility, but can bring additional trauma when the material is taken, thus causing supply area damage. The allograft tissue transplantation material is usually derived from allograft rotator cuff, tibialis anterior tendon, patellar tendon, achilles tendon tissue and the like, although the motion amplitude and muscle strength of the shoulder joint after operation are obviously improved, the allograft tissue transplantation material lacks stable mechanical strength, is easy to generate immune rejection reaction after transplantation, has the risk of infectious diseases and has limited sources. The decellularized tissue graft mainly comprises xenogeneic tissue graft and allogeneic tissue graft, such as animal dermis, small intestine, pericardium and the like, and inherent collagen, non-collagen, three-dimensional structure and the like are taken as a bracket to promote postoperative collagen fiber and blood vessel regeneration and functional reconstruction of tendon-bone interface, although the decellularized tissue graft has good biocompatibility and ductility, the mechanical strength of the decellularized tissue graft cannot meet the mechanical load strength of the rotator cuff.
The current rotator cuff patch material limits the clinical application thereof due to factors such as histocompatibility, biomechanical strength, tissue source and safety. In addition, most of the conventional rotator cuff patches lack the function of inducing tissue regeneration, and particularly lack the function of inducing the formation of a tendon-bone gradient structure, so that the rotator cuff patches which can induce tissue regeneration, realize the formation of a tendon-bone (tendon-fibrocartilage-mineralized fibrocartilage-bone) gradient structure, have excellent mechanical strength and good biocompatibility and have important clinical application value for treating rotator cuff injury are designed.
Disclosure of Invention
The present invention has been made to solve the above problems, and an object of the present invention is to provide an artificial rotator cuff patch capable of inducing the formation of a tendon-bone gradient structure, which includes different inducing factors, and performs different induced differentiation on stem cells to form a tendon-bone (tendon-fibrocartilage-mineralized fibrocartilage-bone) gradient structure, and a method for preparing the same.
The invention provides an artificial rotator cuff patch capable of inducing the formation of a tendon-bone gradient structure, which is characterized by comprising the following components: the bone formation promoting section, the cartilage formation promoting section and the tendon regeneration promoting section are connected in sequence, wherein the bone formation promoting section, the cartilage formation promoting section and the tendon regeneration promoting section are respectively woven into a three-dimensional knitting support through CTGF spinning, KGN spinning and nano-hydroxyapatite spinning, the CTGF spinning, the KGN spinning and the nano-hydroxyapatite spinning respectively comprise a core layer consisting of PET yarns and a gelatin outer layer consisting of gelatin wrapped on the PET yarns, CTGF is uniformly distributed in the gelatin outer layer of the CTGF spinning, KGN is uniformly distributed in the gelatin outer layer of the KGN spinning, and nano-hydroxyapatite is uniformly distributed in the gelatin outer layer of the nano-hydroxyapatite spinning.
The artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure provided by the invention can also have the following characteristics: wherein, the longitudinal section of the core layer is circular, the diameter is 200-260 μm, and the thickness of the gelatin outer layer is 80-150 μm.
The artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure provided by the invention can also have the following characteristics: wherein, in the CTGF spinning, the ratio of CTGF to gelatin in the gelatin outer layer is 50 ng/g-100 ng/g, and in the KGN spinning, the ratio of KGN to gelatin in the gelatin outer layer is 0.5 mu mol/100 g-5 mu mol/100 g.
The artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure provided by the invention can also have the following characteristics: wherein the ratio of the nano-hydroxyapatite in the gelatin outer layer to the gelatin is 20-100 mug/g, and the particle size of the nano-hydroxyapatite is 20-70 nm.
The invention also provides a preparation method of the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure, which is characterized by comprising the following steps: step 1, dissolving gelatin in hexafluoroisopropanol to prepare a gelatin solution, and adding CTGF as an induction factor to obtain CTGF gelatin composite spinning solution; step 2, placing the PET yarns in electrospinning nanofiber yarn preparation equipment as a core layer, spraying the CTGF gelatin composite spinning solution onto the PET yarns, twisting the CTGF gelatin composite spinning solution onto the core layer by using a rotating funnel, and collecting the CTGF gelatin composite spinning solution by using a receiving roller to obtain continuous CTGF spinning; step 3, dissolving gelatin in hexafluoroisopropanol to prepare a gelatin solution, and adding KGN as an induction factor to obtain KGN gelatin composite spinning solution; step 4, placing the PET yarns in electrospinning nanofiber yarn preparation equipment as a core layer, spraying KGN gelatin composite spinning solution onto the PET yarns, twisting the KGN gelatin composite spinning solution onto the core layer by using a rotary funnel, and collecting the KGN gelatin composite spinning solution by using a receiving roller to obtain continuous KGN spinning; step 5, dissolving gelatin in hexafluoroisopropanol to prepare a gelatin solution, and adding nano hydroxyapatite serving as an induction factor to obtain a nano hydroxyapatite and gelatin composite spinning solution; step 6, placing the PET yarns in electrospinning nanofiber yarn preparation equipment as a core layer, spraying the nano hydroxyapatite gelatin composite spinning solution onto the PET yarns, twisting the nano hydroxyapatite gelatin composite spinning solution onto the core layer by using a rotating funnel, and collecting the nano hydroxyapatite gelatin composite spinning solution by using a receiving roller to obtain continuous nano hydroxyapatite spinning; and 7, weaving a three-dimensional knitted support consisting of a bone formation promoting section, a cartilage formation promoting section and a tendon regeneration promoting section which are sequentially connected by a knitting process according to the sequence of CTGF spinning, KGN spinning and nano hydroxyapatite spinning, and thus obtaining the artificial rotator cuff patch capable of inducing the tendon-bone gradient structure to form.
The preparation method of the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure provided by the invention can also have the following characteristics: wherein the gelatin is pigskin gelatin, and the content of the gelatin in the gelatin solution is 0.1 g/ml-0.15 g/ml.
The preparation method of the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure provided by the invention can also have the following characteristics: wherein, in the step 1, the content of CTGF in the CTGF gelatin composite spinning solution is 50 ng/g-100 ng/g, in the step 3, the content of KGN in the KGN gelatin composite spinning solution is 0.5 mu mol/100 g-5 mu mol/100g, in the step 5, the grain diameter of nano-hydroxyapatite is 20 nm-70 nm, and the content of nano-hydroxyapatite in the nano-hydroxyapatite gelatin composite spinning solution is 20 mu g/g-100 mu g/g.
The preparation method of the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure provided by the invention can also have the following characteristics: wherein, in the step 7, the knitting process is a interlock texture process.
The preparation method of the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure provided by the invention can also have the following characteristics: the electrostatic spinning process specifically comprises the following steps: place single yarn in electrostatic spinning nanofiber yarn fixer as the sandwich layer, add CTGF gelatin complex spinning liquid, KGN gelatin complex spinning liquid or nanometer hydroxyapatite gelatin complex spinning liquid in the syringe of both sides, then connect the spinning nozzle at both ends about the PET yarn respectively, two syringes are to spouting, utilize rotatory funnel with in the syringe spun CTGF gelatin complex spinning liquid, KGN gelatin complex spinning liquid or arbitrary one in the nanometer hydroxyapatite gelatin complex spinning liquid twists to the PET yarn on, utilize below to receive the arbor wheel and collect continuous double-deck nanofiber yarn respectively, the parameter of electrostatic spinning technology specifically is: applying positive and negative high pressure of 8 KV-10 KV on the spinning nozzle, keeping the speed of the propulsion pump at 1.2 mL/h-1.5 mL/h, the receiving distance at 12 cm-15 cm, the rotating speed of the rotary funnel at 400 rpm-500 rpm, and the rotating speed of the receiving roller at 8 rpm-10 rpm.
Action and Effect of the invention
According to the preparation method of the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure, provided by the invention, PET yarns are selected as an inner layer, gelatin solutions in which inducing factors of CTGF, KGN and nano-hydroxyapatite are respectively dispersed are selected as an outer layer, and the gelatin solutions are sprayed on the PET yarns by using an electrostatic spinning technology to obtain CTGF spinning, KGN spinning and nano-hydroxyapatite spinning, wherein all the three types of spinning are nano-fiber yarns with double-layer structures. Weaving according to the direction of CTGF-KGN-nano hydroxyapatite to obtain a knitted double-layer structure nano fiber yarn support, namely the artificial rotator cuff patch capable of inducing the tendon-bone gradient structure to form. The inner layer of the knitted double-layer structure nanofiber yarn support is made of PET yarns, and the double-layer structure enables mechanical properties to be better. The small molecular active substances CTGF, KGN and nano-hydroxyapatite are slowly released into local tissues along with the slow degradation of gelatin serving as an outer layer material in vivo to play a role in inducing tissue regeneration for a long time, so that the patch has the function of enhancing the mechanical property of the regenerated tissues for a long time. The outer layer is gelatin, in different parts, CTGF, KGN and nano-hydroxyapatite in the gelatin are respectively used as induction factors, and the induction factors can respectively promote the formation of tendon, cartilage and bone along with the degradation of the gelatin, so that the patch has the function of inducing the formation of a gradient structure of a tendon-bone binding area, and is beneficial to the repair of tendon-bone injury and the recovery of normal functions.
The gelatin is a degradation product of collagen fiber, the gelatin is used as a material, the biocompatibility is better, the induction factors are uniformly mixed in the gelatin spinning solution and are distributed more uniformly, the induction factors mixed in the gelatin can be slowly and durably released along with the degradation of the outer layer of the gelatin, and the action effect is more durable and mild.
The normal tendon-bone tissue binding interface region is a transitional region of tendon-fibrocartilage-bone, and is a structure formed by differentiation of stem cells into tenocytes, differentiation of fibrocartilage cells, and differentiation of bone tissue cells, respectively, and in this example, the tendon-bone tissue binding interface region is simulated by regulating the differentiation of stem cells into the above three cells: formation of the transitional region of "tendon-fibrocartilage-bone".
The preparation method of the artificial rotator cuff patch capable of inducing the tendon-bone gradient structure to form is simple, the obtained three-section artificial rotator cuff patch can promote the tendon-fibrocartilage-bone gradient structure to form, and the artificial rotator cuff patch has good mechanical property and biocompatibility and good clinical application prospect.
Drawings
Fig. 1 is a schematic structural view of an artificial rotator cuff patch in an embodiment of the present invention; and
FIG. 2 is a schematic structural view of a single spinning cross-section in an embodiment of the present invention.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure and the preparation method thereof are specifically described below with reference to the embodiment and the accompanying drawings.
The raw materials and reagents used in the examples of the present invention are commercially available.
The raw material sources in the examples are:
porcine skin Gelatin (Gelatin) is type a Gelatin available from Sigma-Aldrich, usa;
hexafluoroisopropanol (HFIP, available from fine chemicals, ltd, stargardt);
CTGF (connective tissue growth factor, available from Abcam, uk);
KGN (Kartogenin, available from MCE, USA);
nano-Hydroxyapatite (HAP), (hydroxyapatate, available from Sigma-Aldrich, usa).
The electrostatic spinning process comprises the following specific steps: placing single yarn in an electrostatic spinning nanofiber yarn fixer as a core layer, adding any one of gelatin composite spinning solution, namely CTGF gelatin composite spinning solution, KGN gelatin composite spinning solution or nano-hydroxyapatite gelatin composite spinning solution into injectors at two sides, then respectively connecting spinning nozzles at the left end and the right end of the PET yarn, oppositely spraying the two injectors, twisting any one of the CTGF gelatin composite spinning solution, KGN gelatin composite spinning solution or nano-hydroxyapatite gelatin composite spinning solution sprayed out of the injectors onto the PET yarn by using a rotary funnel, and respectively collecting continuous double-layer nanofiber yarns by using a lower receiving shaft wheel.
The parameters of the electrostatic spinning process are as follows: applying positive and negative high pressure of 8 KV-10 KV on the spinning nozzle, keeping the speed of the propulsion pump at 1.2 mL/h-1.5 mL/h, the receiving distance at 12 cm-15 cm, the rotating speed of the rotary funnel at 400 rpm-500 rpm, and the rotating speed of the receiving roller at 8 rpm-10 rpm.
< example >
The structure and the preparation process of the artificial rotator cuff patch capable of inducing the tendon-bone gradient structure to form are specifically described in the embodiment.
The preparation method of the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure comprises the following steps:
The specific operation is as follows: the Gelatin is selected from pigskin Gelatin, 1.5g of pigskin Gelatin (Gelatin) is weighed and dissolved in 10mL to prepare Gelatin spinning solution (Ge), wherein the content of the Gelatin is 0.15 g/mL. And then adding the CTGF into a gelatin solution, wherein the content of the CTGF in the CTGF gelatin composite spinning solution is 50 ng/g-100 ng/g. In this example, the CTGF content was 50 ng/g.
The specific operation is as follows: KGN is selected as an induction factor, the KGN content in the KGN gelatin composite spinning solution is 0.5-5 [ mu ] mol/100g, and the rest of the operation is the same as the step 1 and is not repeated. In this example, the KGN content was 3. mu. mol/100 g.
Step 4, preparing the PET-Ge double-layer nanofiber yarn: putting the PET (ethylene terephthalate) yarn into electrospinning nanofiber yarn preparation equipment as a core layer, spraying KGN gelatin composite spinning solution onto the PET (ethylene terephthalate) yarn, twisting the KGN gelatin composite spinning solution onto the PET yarn, namely the core layer, by using a rotating funnel, and collecting by using a receiving roller to obtain continuous KGN spinning.
Step 5, preparing a gelatin composite spinning solution: dissolving gelatin in hexafluoroisopropanol to prepare a gelatin solution, and adding nano hydroxyapatite serving as an induction factor to obtain the nano hydroxyapatite and gelatin composite spinning solution.
The specific operation is as follows: selecting nano-hydroxyapatite with the particle size of 20 nm-70 nm as an induction factor, wherein the content of the nano-hydroxyapatite in the nano-hydroxyapatite gelatin composite spinning solution is 20 mu g/g-100 mu g/g, and the rest operations are the same as the step 1 and are not repeated. In this example, nano hydroxyapatite with a particle size of 50nm was selected and contained in the gelatin composite spinning solution at a content of 50 μ g/g.
Step 6, preparing the PET-Ge double-layer nanofiber yarn: putting the PET (ethylene terephthalate) yarn into electrospinning nanofiber yarn preparation equipment as a core layer, spraying the nano-hydroxyapatite gelatin composite spinning solution onto the PET (ethylene terephthalate) yarn, twisting the nano-hydroxyapatite gelatin composite spinning solution onto the PET yarn, namely the core layer, by using a rotating funnel, and collecting by using a receiving roller to obtain continuous nano-hydroxyapatite spinning.
And 7, weaving a three-dimensional knitted support consisting of a bone formation promoting section, a cartilage formation promoting section and a tendon regeneration promoting section which are sequentially connected by a knitting process according to the sequence of CTGF spinning, KGN spinning and nano hydroxyapatite spinning, and thus obtaining the artificial rotator cuff patch capable of inducing the tendon-bone gradient structure to form.
The specific operation is as follows: the LXC-352CVI computerized flat knitting machine is used for knitting single continuous PET-Ge double-layer nano-fiber yarns into a three-dimensional knitting support by adopting a interlock texture process according to the sequence of CTGF spinning, KGN spinning and nano-hydroxyapatite spinning.
Fig. 1 is a structural view of an artificial rotator cuff patch in an embodiment of the present invention, and fig. 2 is a structural view of a single spun cross-section in an embodiment of the present invention.
As shown in fig. 1 and 2, the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure is a three-dimensional knitted scaffold and consists of a bone formation promoting segment a, a cartilage formation promoting segment B and a tendon regeneration promoting segment C which are sequentially connected.
The bone formation promoting section A, the cartilage formation promoting section B and the tendon regeneration promoting section C are respectively formed by CTGF spinning, KGN spinning and nano hydroxyapatite spinning and woven, and the CTGF spinning, the KGN spinning and the nano hydroxyapatite spinning respectively comprise a core layer 1 formed by PET yarns, a gelatin outer layer 2 formed by gelatin wrapped on the PET yarns and induction factors 3 uniformly dispersed in the gelatin outer layer. The longitudinal section of the core layer 1 is circular, the diameter is 200-260 μm, and the thickness of the gelatin outer layer 2 is 80-150 μm. The inducing factor 3 is granular and is CTGF, KGN and nano hydroxyapatite.
In this embodiment, the core layer 1 is made of a thicker single PET yarn formed by bundling a plurality of PET filaments 11, which is beneficial to increasing the mechanical properties of the single PET yarn.
CTGF is uniformly distributed in the gelatin outer layer of the CTGF spinning, and the ratio of the CTGF to the gelatin is 50 ng/g-100 ng/g. CTGF is an induction factor that promotes differentiation of stem cells toward the tendon and promotes formation of the tendon.
KGN is uniformly distributed in the gelatin outer layer of the KGN spinning, and the ratio of KGN to gelatin is 0.5-5 [ mu ] mol/100 g. KGN is an inducing factor that promotes differentiation of stem cells into cartilage and promotes cartilage formation.
The nanometer hydroxyapatite is uniformly distributed in the gelatin outer layer of the nanometer hydroxyapatite spinning, and the ratio of the nanometer hydroxyapatite to the gelatin is 20-100 mug/g. The nano hydroxyapatite is used as an induction factor, can promote stem cells to differentiate towards the bone direction, and promotes the formation of the bone.
The inner layer of the artificial rotator cuff patch is made of PET material, and the outer layer of the artificial rotator cuff patch is made of gelatin spun on the surface of PET. The differences of the three parts of braided fabrics of the bone formation promoting section A, the cartilage formation promoting section B and the tendon regeneration promoting section C lie in that different induction factors mixed in gelatin spinning solution are different, different induction factors are loaded on patches of different parts, stem cells are respectively subjected to different induced differentiation, the formation of a tendon-bone (tendon-fibrocartilage-bone) gradient structure is realized, and finally a gradient structure close to a natural tendon-bone is formed.
When the operation for repairing the rotator cuff tear is carried out, the artificial rotator cuff patch capable of inducing the tendon-bone gradient structure to form is placed at the rotator cuff tear, so that the bone formation promoting section A is close to the bone end, and the tendon regeneration promoting section C is close to the tendon end. With the degradation of the gelatin on the outer layer, the inducing factors mixed in the gelatin are slowly and durably released, and CTGF, KGN and nano-hydroxyapatite which are used as the inducing factors respectively play respective roles, so that stem cells are respectively differentiated towards the directions of tendon, cartilage and bone to form a gradient structure close to nature, and the rotator cuff repair process is completed. However, due to the characteristics of the induction factors CTGF, KGN and nano-hydroxyapatite, attention needs to be paid to ethylene oxide sterilization during sterilization to avoid the inactivation of the induction factors such as CTGF and the like.
Effects and effects of the embodiments
According to the preparation method of the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure, provided by the embodiment, a PET yarn is selected as an inner layer, gelatin solutions in which inducing factors of CTGF, KGN and nano-hydroxyapatite are respectively dispersed are selected as outer layers, and the gelatin solutions are sprayed on the PET yarn by using an electrostatic spinning technology to obtain CTGF spinning, KGN spinning and nano-hydroxyapatite spinning, wherein all the three types of spinning are nano-fiber yarns with a double-layer structure. Weaving according to the direction of CTGF-KGN-nano hydroxyapatite to obtain a knitted double-layer structure nano fiber yarn support, namely the artificial rotator cuff patch capable of inducing the tendon-bone gradient structure to form. The inner layer of the knitted double-layer structure nanofiber yarn support is made of PET yarns, and the double-layer structure enables mechanical properties to be better. The small molecular active substances CTGF and KGN and the nano-hydroxyapatite in the material are slowly released into local tissues along with the slow degradation of gelatin as an outer layer material in vivo, and play a role in inducing tissue regeneration for a long time, so that the patch has the function of enhancing the mechanical property of the regenerated tissues for a long time. The outer layer is gelatin, in different parts, CTGF, KGN and nano-hydroxyapatite in the gelatin are respectively used as induction factors, and the induction factors can respectively promote the formation of tendon, cartilage and bone along with the degradation of the gelatin, so that the patch has the function of inducing the formation of a gradient structure of a tendon-bone binding area, and is beneficial to the repair of tendon-bone injury and the recovery of normal functions.
The gelatin is a degradation product of collagen fiber, the gelatin is used as a material, the biocompatibility is better, the induction factors are uniformly mixed in the gelatin spinning solution and are distributed more uniformly, the induction factors mixed in the gelatin can be slowly and durably released along with the degradation of the outer layer of the gelatin, and the action effect is more durable and mild.
The normal tendon-bone tissue binding interface region is a transitional region of tendon-fibrocartilage-bone, and is a structure formed by differentiation of stem cells into tenocytes, differentiation of fibrocartilage cells, and differentiation of bone tissue cells, respectively, and in this example, the tendon-bone tissue binding interface region is simulated by regulating the differentiation of stem cells into the above three cells: formation of the transitional region of "tendon-fibrocartilage-bone".
In addition, when the content of gelatin in the gelatin solution is 0.1-0.15 g/ml, namely the concentration is 1-1.5%, the gelling performance is good, and the gelatin solution is not too viscous, so that the electrostatic spinning nozzle is prevented from being blocked, and the normal preparation of the nanofiber is ensured.
In addition, the CTGF content in the CTGF gelatin composite spinning solution is 50 ng/g-100 ng/g, and the CTGF content can exert a relatively ideal effect of promoting the differentiation of cell tenoblasts when the concentration is in the range; the concentration is too low, and the better effect cannot be exerted after the release to the local part; too high a concentration of drug results in too high a concentration of drug around the stent, resulting in cell degeneration or not better results, and drug waste.
In addition, the KGN gelatin composite spinning solution has KGN content of 0.5-5 micromole/100 g, and can promote chondrogenic differentiation of cells in the concentration range; the concentration is too low, and the better effect cannot be exerted after the release to the local part; too high a concentration of drug results in too high a concentration of drug around the stent, resulting in cell degeneration or not better results, and drug waste.
In addition, the particle size of the nano hydroxyapatite is 20-70 nm, and the content of the nano hydroxyapatite in the nano hydroxyapatite gelatin composite spinning solution is 20-100 mug/g. The grain diameter is 20 nm-70 nm, so that the uniform nanofiber scaffold with stable structure can be conveniently spun; within the concentration range, the compound can exert relatively ideal effect of promoting cell osteogenic differentiation; the concentration is too low, and the better effect cannot be exerted after the release to the local part; too high a concentration results in too high a concentration of drug around the stent, resulting in cell degeneration or no better effect and material waste.
The preparation method of the artificial rotator cuff patch capable of inducing the tendon-bone gradient structure to form is simple, the obtained three-section artificial rotator cuff patch can promote the tendon-fibrocartilage-bone gradient structure to form, and the artificial rotator cuff patch has good mechanical property, biocompatibility and good clinical application prospect.
The above embodiments are preferred examples of the present invention, and are not intended to limit the scope of the present invention.
Claims (9)
1. An artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation, comprising:
a bone formation promoting section, a cartilage formation promoting section and a tendon regeneration promoting section which are connected in sequence,
wherein the bone formation promoting section, the cartilage formation promoting section and the tendon regeneration promoting section are respectively woven into a three-dimensional knitted scaffold by CTGF spinning, KGN spinning and nano hydroxyapatite spinning,
the CTGF spinning, the KGN spinning and the nano hydroxyapatite spinning respectively comprise a core layer consisting of PET yarns and a gelatin outer layer consisting of gelatin coated on the PET yarns,
CTGF is uniformly distributed in the gelatin outer layer of the CTGF spinning,
KGN is uniformly distributed in the gelatin outer layer of the KGN spinning,
and nano hydroxyapatite is uniformly distributed in the gelatin outer layer of the nano hydroxyapatite spinning.
2. An artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation according to claim 1 wherein:
wherein the longitudinal section of the core layer is circular, the diameter is 200-260 μm,
the thickness of the gelatin outer layer is 80-150 μm.
3. An artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation according to claim 1 wherein:
wherein, in the CTGF spinning, the ratio of the CTGF to the gelatin in the gelatin outer layer is 50 ng/g-100 ng/g,
in the KGN spinning, the ratio of KGN in the gelatin outer layer to gelatin is 0.5-5 [ mu ] mol/100 g.
4. An artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation according to claim 1 wherein:
wherein the ratio of the nano hydroxyapatite in the gelatin outer layer to the gelatin is 20-100 mug/g, and the particle size of the nano hydroxyapatite is 20-70 nm.
5. A preparation method of the artificial rotator cuff patch capable of inducing the formation of the tendon-bone gradient structure as claimed in any one of claims 1 to 4, which comprises the following steps:
step 1, dissolving the gelatin in hexafluoroisopropanol to prepare a gelatin solution, and adding the CTGF as an induction factor to obtain a CTGF gelatin composite spinning solution;
step 2, placing the PET yarns in electrospinning nanofiber yarn preparation equipment to serve as the core layer, spraying the CTGF gelatin composite spinning solution onto the PET yarns, twisting the CTGF gelatin composite spinning solution onto the core layer by using a rotating funnel, and collecting the CTGF gelatin composite spinning solution by using a receiving roller to obtain continuous CTGF spinning;
step 3, dissolving the gelatin in the hexafluoroisopropanol to prepare the gelatin solution, and adding the KGN as an induction factor to obtain a KGN gelatin composite spinning solution;
step 4, placing the pair of PET yarns in the electrospinning nanofiber yarn preparation equipment as the core layer, spraying the KGN gelatin composite spinning solution onto the PET yarns, twisting the KGN gelatin composite spinning solution onto the core layer by using the rotary hopper, and collecting the KGN gelatin composite spinning solution by using a receiving roller to obtain continuous KGN spinning;
step 5, dissolving the gelatin in the hexafluoroisopropanol to prepare a gelatin solution, and adding the nano-hydroxyapatite as an induction factor to obtain a nano-hydroxyapatite and gelatin composite spinning solution;
step 6, placing the PET yarns in the electrospinning nanofiber yarn preparation equipment as the core layer, spraying the nano hydroxyapatite gelatin composite spinning solution onto the PET yarns, twisting the nano hydroxyapatite gelatin composite spinning solution onto the core layer by using the rotary hopper, and collecting the twisted nano hydroxyapatite gelatin composite spinning solution by using a receiving roller to obtain continuous nano hydroxyapatite spinning;
and 7, weaving a three-dimensional knitted scaffold consisting of a bone formation promoting section, a cartilage formation promoting section and a tendon regeneration promoting section which are sequentially connected by a knitting process according to the sequence of the CTGF spinning, the KGN spinning and the nano hydroxyapatite spinning, namely the artificial rotator cuff patch capable of inducing the tendon-bone gradient structure to form.
6. The method for preparing an artificial rotator cuff patch capable of inducing the formation of a tendon-bone gradient structure according to claim 5, wherein the method comprises the following steps:
wherein the gelatin is pigskin gelatin, and the content of the gelatin in the gelatin solution is 0.1 g/ml-0.15 g/ml.
7. The method for preparing an artificial rotator cuff patch capable of inducing the formation of a tendon-bone gradient structure according to claim 6, wherein the method comprises the following steps:
wherein in the step 1, the CTGF content in the CTGF gelatin composite spinning solution is 50 ng/g-100 ng/g,
in the step 3, the KGN content in the KGN gelatin composite spinning solution is 0.5-5 [ mu ] mol/100g,
in the step 5, the particle size of the nano hydroxyapatite is 20 nm-70 nm, and the content of the nano hydroxyapatite in the nano hydroxyapatite and gelatin composite spinning solution is 20 mug/g-100 mug/g.
8. The method for preparing an artificial rotator cuff patch capable of inducing the formation of a tendon-bone gradient structure according to claim 5, wherein the method comprises the following steps:
wherein, in the step 7, the knitting process is a interlock texture process.
9. The method for preparing an artificial rotator cuff patch capable of inducing the formation of a tendon-bone gradient structure according to claim 5, wherein the method comprises the following steps:
the electrostatic spinning process specifically comprises the following steps: putting a single yarn in an electrostatic spinning nanofiber yarn fixer as a core layer, adding any one of the CTGF gelatin composite spinning solution, the KGN gelatin composite spinning solution or the nano-hydroxyapatite gelatin composite spinning solution into injectors at two sides, then respectively connecting spinning nozzles at the left end and the right end of the PET yarn, spraying the two injectors in opposite directions, twisting any one of the CTGF gelatin composite spinning solution, the KGN gelatin composite spinning solution or the nano-hydroxyapatite gelatin composite spinning solution sprayed out of the injectors onto the PET yarn by using a rotary funnel, respectively collecting continuous double-layer nanofiber yarns by using a lower receiving shaft wheel,
the parameters of the electrostatic spinning process are as follows: applying positive and negative high pressure of 8-10 KV on the spinning nozzle, keeping the speed of a propulsion pump at 1.2-1.5 mL/h, the receiving distance at 12-15 cm, the rotating speed of the rotating funnel at 400-500 rpm, and the rotating speed of the receiving roller at 8-10 rpm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010330989.8A CN111359012A (en) | 2020-04-24 | 2020-04-24 | Artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010330989.8A CN111359012A (en) | 2020-04-24 | 2020-04-24 | Artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111359012A true CN111359012A (en) | 2020-07-03 |
Family
ID=71199987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010330989.8A Pending CN111359012A (en) | 2020-04-24 | 2020-04-24 | Artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111359012A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113171493A (en) * | 2021-04-08 | 2021-07-27 | 中国人民解放军总医院第一医学中心 | Biological patch and preparation method thereof |
| CN114366850A (en) * | 2022-01-19 | 2022-04-19 | 复旦大学附属华山医院 | Artificial rotator cuff patch based on drug-loaded silk protein and preparation method thereof |
| CN114732948A (en) * | 2022-03-25 | 2022-07-12 | 上海工程技术大学 | Rotator cuff patch and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4149277A (en) * | 1977-06-22 | 1979-04-17 | General Atomic Company | Artificial tendon prostheses |
| CN104096272A (en) * | 2013-04-03 | 2014-10-15 | 中国科学院化学研究所 | Postoperation anti-infectious composite electrostatic-spinning nanometer fiber-film sheet for repairing hernia and preparation method thereof |
| CN107636220A (en) * | 2015-06-17 | 2018-01-26 | 广州迈普再生医学科技有限公司 | One kind enhancing electrospun fiber membrane and preparation method thereof |
| CN110331486A (en) * | 2019-07-25 | 2019-10-15 | 东华大学 | A kind of multilayered structure nano-fibre yams knitting tendon scaffold and its preparation and application |
| CN110344151A (en) * | 2019-07-25 | 2019-10-18 | 东华大学 | Simulate the biomimetic scaffolds and preparation method thereof of natural tendon tissue fiber hierarchical structure |
-
2020
- 2020-04-24 CN CN202010330989.8A patent/CN111359012A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4149277A (en) * | 1977-06-22 | 1979-04-17 | General Atomic Company | Artificial tendon prostheses |
| CN104096272A (en) * | 2013-04-03 | 2014-10-15 | 中国科学院化学研究所 | Postoperation anti-infectious composite electrostatic-spinning nanometer fiber-film sheet for repairing hernia and preparation method thereof |
| CN107636220A (en) * | 2015-06-17 | 2018-01-26 | 广州迈普再生医学科技有限公司 | One kind enhancing electrospun fiber membrane and preparation method thereof |
| CN110331486A (en) * | 2019-07-25 | 2019-10-15 | 东华大学 | A kind of multilayered structure nano-fibre yams knitting tendon scaffold and its preparation and application |
| CN110344151A (en) * | 2019-07-25 | 2019-10-18 | 东华大学 | Simulate the biomimetic scaffolds and preparation method thereof of natural tendon tissue fiber hierarchical structure |
Non-Patent Citations (1)
| Title |
|---|
| 陆定贵等: "关节镜下后交叉韧带损伤重建技术研究进展", 《右江医学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113171493A (en) * | 2021-04-08 | 2021-07-27 | 中国人民解放军总医院第一医学中心 | Biological patch and preparation method thereof |
| CN114366850A (en) * | 2022-01-19 | 2022-04-19 | 复旦大学附属华山医院 | Artificial rotator cuff patch based on drug-loaded silk protein and preparation method thereof |
| CN114732948A (en) * | 2022-03-25 | 2022-07-12 | 上海工程技术大学 | Rotator cuff patch and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Silva et al. | Biodegradable polymer nanocomposites for ligament/tendon tissue engineering | |
| US7108721B2 (en) | Tissue regrafting | |
| CN111359012A (en) | Artificial rotator cuff patch capable of inducing tendon-bone gradient structure formation and preparation method thereof | |
| Koh et al. | In vivo study of novel nanofibrous intra-luminal guidance channels to promote nerve regeneration | |
| Saveh-Shemshaki et al. | Nanofiber-based matrices for rotator cuff regenerative engineering | |
| Salehi et al. | Fabrication and characterization of electrospun PLLA/collagen nanofibrous scaffold coated with chitosan to sustain release of aloe vera gel for skin tissue engineering | |
| Tuzlakoglu et al. | Biodegradable polymeric fiber structures in tissue engineering | |
| EP2373354B1 (en) | Tissue repair scaffold | |
| CN111450316B (en) | Integrated bracket for simulating bone-tendon-bone mineralization-non-mineralization gradient structure | |
| Mo et al. | Electrospun nanofibers for tissue engineering | |
| CN108403258B (en) | Dead point reconstruction type artificial rotator cuff patch and manufacturing method thereof | |
| EP1282452A1 (en) | Tissue regrafting | |
| Mao et al. | A systematic review of tissue engineering scaffold in tendon bone healing in vivo | |
| JP2003512896A (en) | Tissue repair | |
| Tang et al. | Functional biomaterials for tendon/ligament repair and regeneration | |
| CN111544654B (en) | Composite artificial ligament and manufacturing method thereof | |
| Obregón et al. | Nanofiber composites in blood vessel tissue engineering | |
| CN114732948A (en) | Rotator cuff patch and preparation method thereof | |
| Song et al. | Bioinspired protein/peptide loaded 3D printed PLGA scaffold promotes bone regeneration | |
| Zhang et al. | Research progress of biodegradable polymers in repairing Achilles tendon injury | |
| JP2019515758A (en) | Osteoinductive fibrous bone chip | |
| Anjana et al. | Nanoengineered biomaterials for tendon/ligament regeneration | |
| Lopiz et al. | Repair of rotator cuff injuries using different composites | |
| CN108744065B (en) | Tissue repair stent and preparation method and application thereof | |
| CN115605236B (en) | Composite product for cartilage damaged bone joint regeneration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200703 |
|
| RJ01 | Rejection of invention patent application after publication |