CN111349552B - Miniature aerosol virus sampler - Google Patents
Miniature aerosol virus sampler Download PDFInfo
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- CN111349552B CN111349552B CN202010282244.9A CN202010282244A CN111349552B CN 111349552 B CN111349552 B CN 111349552B CN 202010282244 A CN202010282244 A CN 202010282244A CN 111349552 B CN111349552 B CN 111349552B
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- aerosol
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- sampler
- humidification
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- 239000000443 aerosol Substances 0.000 title claims abstract description 80
- 241000700605 Viruses Species 0.000 title claims abstract description 53
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 239000000945 filler Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 8
- 238000000034 method Methods 0.000 description 9
- 238000005070 sampling Methods 0.000 description 7
- 241000711573 Coronaviridae Species 0.000 description 6
- 238000013461 design Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000010419 fine particle Substances 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002498 deadly effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000010460 detection of virus Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 229940059904 light mineral oil Drugs 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application discloses a miniature aerosol virus sampler which comprises a collecting box, wherein a plurality of gas inlets are formed at the top of the collecting box and the top of a side wall of the collecting box, an aerosol humidifying box is arranged in the collecting box, a humidifying channel is formed along the vertical axis of the aerosol humidifying box, a water absorbing filler is formed on the inner wall of the humidifying channel, an aerosol liquid medium collision collector is formed at the bottom of the humidifying channel, a transversely arranged vacuum pump air outlet channel is formed between the aerosol liquid medium collision collector and the bottom of the humidifying channel, and an outflow capillary is formed at the bottom of the aerosol liquid medium collision collector. The application can be used to collect a sample of virus in an aerosol in a liquid medium, and subsequently, the collected sample can be subjected to virus detection using PCR by pretreatment.
Description
Technical Field
The application relates to virus sampling, in particular to a miniature aerosol virus sampler.
Background
Viruses transmitted by aerosols (which may include novel coronaviruses) are severely compromised for human health, presenting a significant challenge to the healthcare system. Today laboratory detection of viruses is mostly focused on respiratory tract samples due to the limitations of sample sampling. Direct detection from air, particularly from aerosol samples, would be the most desirable method.
Furthermore, aerosol transmission is one of the important transmission pathways for bacterial viruses, including novel coronaviruses. The propagation distance of the aerosol is far, and the harm is difficult to prevent and control. Detecting the presence of new coronaviruses in the air is also very difficult due to the limitations of aerosol sampling techniques involving viruses. And thus the true hazard of the new coronavirus aerosol propagation pathway cannot be correctly assessed.
Aerosol sampling techniques containing viruses can be classified into 4 categories according to principle: solid impactor, liquid impactor, filter and electrostatic collector.
Problems with these conventional techniques include: (1) the collection efficiency for fine particles is quite low; (2) viral dehydration caused during collection; (3) damage to the virus at the impact of the solid collector; (4) The virus aerosol collected on the medium is atomized again; (5) the collector is heavy and is not suitable for carrying at any time; (6) the collection flow is small, and the sampling time is long; (7) the use safety is not strong.
Disclosure of Invention
The invention aims to provide a miniature aerosol virus sampler which can be used for collecting a virus sample in aerosol in a liquid medium, and then, the collected sample can be subjected to virus detection by PCR (polymerase chain reaction) through pretreatment or combined with an online biosensor to form a real-time online aerosol virus sampling and detector.
In order to achieve the above purpose, the present invention provides the following technical solutions.
The embodiment of the application discloses a miniature aerosol virus sampler which comprises a collecting box, wherein a plurality of gas inlets are formed at the top of the collecting box and the top of a side wall of the collecting box, an aerosol humidifying box is arranged in the collecting box, a humidifying channel is formed along the vertical axis of the aerosol humidifying box, a water absorbing filler is formed on the inner wall of the humidifying channel, an aerosol liquid medium collision collector is formed at the bottom of the humidifying channel, a transversely arranged vacuum pump air outlet channel is formed between the aerosol liquid medium collision collector and the bottom of the humidifying channel, and an outflow capillary is formed at the bottom of the aerosol liquid medium collision collector.
Preferably, in the micro aerosol virus sampler, a pinhole nozzle is formed at the gas inlet.
Preferably, in the micro aerosol virus sampler, three gas inlets are provided and are respectively disposed at the top of the collection box and at the tops of two opposite side walls.
Preferably, in the micro aerosol virus sampler, the virus-containing aerosol absorbs the moisture in the humidifying channel, becomes larger in size, and is trapped in the aerosol liquid medium collision collector and flows out of the outflow capillary.
Preferably, in the micro aerosol virus sampler described above, the outflow capillary is connected to an in-line biosensor.
Preferably, in the miniature aerosol virus sampler, a thermocouple is wrapped in a shell of the collection box, and the collection box is heated to 60 ℃ at constant temperature.
Preferably, in the micro aerosol virus sampler, the gas flow rate in the vacuum pump air outlet channel is 800-1500L/min.
Compared with the prior art, the virus sampler has the advantages that the designed virus sampler has the following advantages:
(1) The volume is small, the weight is light, and the carrying is convenient;
(2) The aerosol has full particle size and high collection efficiency of fine particles;
(3) The flow is large, and the acquisition time is short;
(4) The safety is strong;
(5) The collected virus has little loss and high activity.
The technical scheme combines with the off-line PCR detection technology, and can be applied to relatively closed public spaces (such as elevators and the like) of medical epidemic prevention forestocks, offices, schools and residential buildings. The sampler can be integrated in a personal biosensor and connected with a smart phone, so that the real-time on-line monitoring of the virus or bacteria concentration in the surrounding environment of the person is possible.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings may be obtained according to the drawings without inventive effort to those skilled in the art.
Fig. 1 is a schematic diagram of a micro aerosol virus sampler in an embodiment of the invention.
Detailed Description
The following detailed description of the technical solutions according to the embodiments of the present invention will be given with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Referring to fig. 1, the micro aerosol virus sampler includes a collection box 100, three gas inlets 101 are formed at the top of the collection box 100 and at the tops of two opposite side walls, an aerosol humidification box 102 is disposed in the collection box 100, a humidification channel 103 is formed along the vertical axis of the aerosol humidification box 102, a water absorbing filler 104 is formed on the inner wall of the humidification channel 103, an aerosol liquid medium collision collector 105 is formed at the bottom of the humidification channel 103, a transversely-disposed vacuum pump outlet channel 106 is formed between the aerosol liquid medium collision collector 105 and the bottom of the humidification channel 103, and an outflow capillary 107 is formed at the bottom of the aerosol liquid medium collision collector 105. A pinhole nozzle is formed at the gas inlet 101. The moisture in the virus-containing aerosol absorption humidification channel 103 becomes larger in size and then is trapped in the aerosol liquid medium impinging collector 105 and flowing out of the outflow capillary 107. The outflow capillary 107 is connected to an in-line biosensor. The housing of the collection box 100 is internally wrapped with a thermocouple 108 and the collection box 100 is heated to 60 ℃ at constant temperature. The flow rate of the gas in the vacuum pump outlet passage 106 is 800-1500L/min.
In the technical scheme, aerosol with viruses passes through 3 nozzles, and aerosol containing viruses and bacteria (with the size of 0.01 to 100 microns) is sucked into a collection box through a high-flow vacuum pump (1000L/min) arranged above the liquid surface of an aerosol liquid medium collision collector. Subsequently, the aerosol passes through an aerosol humidification tank, absorbing the moisture stored in the water-absorbing filler to grow in size. The aerosol after size increase is fed into a liquid medium (light mineral oil of the biomolecular grade) and the aerosol particles with viruses are trapped in the liquid by impact and inertia etc. Finally, the liquid with virus flows out through the capillary tube where the aerosol liquid hits the bottom of the collector. The liquid sample can be directly taken out for off-line PCR detection of the new coronavirus or combined with an on-line biosensor to become a personal portable new coronavirus alarm. The aerosol humidifying box increases the size of the aerosol, thereby greatly improving the collection efficiency. The shell of the collecting box is wrapped by a thermocouple, and the thermocouple is heated at a constant temperature of 60 ℃ to protect the safety of a user.
The innovation points of the technical scheme are as follows:
(1) Portable: small volume, light weight and convenient carrying.
(2) The safety is strong: the human body safety protection shell is adopted to keep the temperature at 60 ℃, so that the safety of a user is comprehensively protected.
(3) The collection efficiency is high: the aerosol humidification growth technology is adopted, so that the collection efficiency of fine particles is greatly improved.
(4) Aerosol size range is full: aerosol as small as 0.01 microns and spray as large as 100 microns can be collected.
(5) The acquisition time is short: the large flow design enables collection to be completed in a short time.
(6) The liquid is used as a virus collection medium, so that the collected virus has small loss and high activity.
The core technology of this scheme is a novel efficient miniature liquid impact virus's that has a plurality of nozzles collector:
(1) Liquid impactors are the most commonly used samplers for collecting aerosolized virus. The use of a liquid as a medium for collection of the virus helps to maintain the activity of the virus, reduce the loss of virus, and facilitate rapid detection. Like other collection means-filters, etc., reagents are required to extract the virus enriched on the surface or on the filter.
(2) Viruses that are infectious by air are present on aerosols of various sizes. The multi-nozzle design in the sampler makes it possible to collect aerosols of a wide range of sizes: aerosol particles as small as 0.01 microns and droplets as large as 100 microns can be collected. Nozzle samplers in the form of holes or slits require a vacuum pump to draw in the aerosol sample. When a sample is drawn into the nozzle device, particles with high inertial impaction will collect in the liquid medium.
(3) Because the content of viruses in aerosol is not high, the existing sampler cannot collect enough samples for subsequent detection. The sampler adopts a large-flow design, so that enough samples can be acquired in a short time for subsequent detection.
(4) Aerosol collection efficiency generally decreases as the size of the aerosol decreases. While smaller size aerosols stay longer in the air and travel longer distances and are therefore more harmful. The design adopts aerosol humidification growth technology, and greatly improves the collection efficiency of pathogens in small-size aerosol which is more harmful to human bodies.
(5) Viruses are deadly pathogens. The design adopts the human body safety protection shell to ensure the safety of a user. The protective shell is set at a constant temperature of 60 ℃, so that the infection of a user of the sampler by pathogens with enriched concentration is avoided.
(6) The sampler adopts a miniature design, has small volume and light weight, and is more convenient to carry about for sampling.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
The foregoing is merely illustrative of the embodiments of this application and it will be appreciated by those skilled in the art that variations and modifications may be made without departing from the principles of the application, and it is intended to cover all modifications and variations as fall within the scope of the application.
Claims (5)
1. The utility model provides a miniature aerosol virus sample thief, its characterized in that includes a collection box, collection box top and lateral wall top are formed with a plurality of gas inlets, collection box inside is provided with aerosol humidification case, aerosol humidification case is formed with the humidification passageway along its vertical axle center, humidification passageway inner wall is formed with the filler that absorbs water, humidification passageway bottom is formed with aerosol liquid medium collision collector, aerosol liquid medium collision collector with be formed with the vacuum pump air outlet channel of horizontal setting between the humidification passageway bottom, aerosol liquid medium collision collector bottom is formed with out the capillary, contains the virus aerosol absorption moisture size grow back is held back in the aerosol liquid medium collision collector in the humidification passageway, follow after the collection out in the capillary, out the capillary is connected to online biosensor.
2. The micro aerosol virus sampler of claim 1, wherein the gas inlet forms a pinhole nozzle.
3. The miniature aerosol virus sampler of claim 1, wherein three gas inlets are provided, one at each of the top of the collection box and the top of the opposite side walls.
4. The miniature aerosol virus sampler of claim 1, wherein a thermocouple is wrapped in the housing of the collection tank and the collection tank is thermostatically heated to 60 ℃.
5. The micro aerosol virus sampler of claim 1, wherein the gas flow rate in the vacuum pump outlet channel is 800-1500L/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202010282244.9A CN111349552B (en) | 2020-04-11 | 2020-04-11 | Miniature aerosol virus sampler |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010282244.9A CN111349552B (en) | 2020-04-11 | 2020-04-11 | Miniature aerosol virus sampler |
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| CN111349552A CN111349552A (en) | 2020-06-30 |
| CN111349552B true CN111349552B (en) | 2024-07-26 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112725160B (en) * | 2021-03-10 | 2025-01-28 | 江苏省人民医院（南京医科大学第一附属医院） | A collection and enrichment system for microbial aerosols |
| CN114018658B (en) * | 2021-11-09 | 2022-06-21 | 中国人民解放军总医院第五医学中心 | An air sample collector for regional infectious disease detection equipment |
| CN115595244A (en) * | 2022-10-14 | 2023-01-13 | 西安建筑科技大学（Cn） | Warm-body dummy, method, apparatus and medium for sampling environmental virus of warm-body dummy |
| CN115902088B (en) * | 2023-03-09 | 2023-05-16 | 合肥泷睿机器人有限责任公司 | Nanometer titanium dioxide photocatalysis screen virus killing performance testing device |
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| CN212669687U (en) * | 2020-04-11 | 2021-03-09 | 苏州智爱环境科技有限公司 | Miniature aerosol virus sampler |
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| WO2009026130A2 (en) * | 2007-08-16 | 2009-02-26 | University Of Florida Research Foundation, Inc. | High-efficiency viable sampler for ultrafine bioaerosols |
| US10859473B2 (en) * | 2016-04-06 | 2020-12-08 | University Of Florida Research Foundation, Inc. | Bioaerosol detection systems and methods of use |
| CN108387410B (en) * | 2018-03-30 | 2024-07-19 | 北京大学 | Wet-wall cyclone type large flow air sampler enhanced by electrostatic field and gravity centrifugal force |
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| CN212669687U (en) * | 2020-04-11 | 2021-03-09 | 苏州智爱环境科技有限公司 | Miniature aerosol virus sampler |
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