CN111349544A - Algae aeration culture device - Google Patents

Algae aeration culture device Download PDF

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Publication number
CN111349544A
CN111349544A CN202010171366.0A CN202010171366A CN111349544A CN 111349544 A CN111349544 A CN 111349544A CN 202010171366 A CN202010171366 A CN 202010171366A CN 111349544 A CN111349544 A CN 111349544A
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wall
funnel
bottle body
bottle
algae
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CN202010171366.0A
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CN111349544B (en
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石彭灵
刘兰海
杨品红
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Hunan University of Arts and Science
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Hunan University of Arts and Science
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management

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  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses an algae aeration culture device which comprises a bottle body, wherein a bottle mouth is arranged at the top of the bottle body, threads are arranged on the circumferential outer wall of the bottle mouth, an installation ring is in threaded connection with the circumferential position of the bottle mouth, two rubber plugs are arranged on the circumferential inner wall of the installation ring, an air outlet pipe and an air inlet pipe are inserted into the tops of the two rubber plugs, a limiting ring is arranged on the circumferential position of the outer wall of the top of the bottle body, an oil seal cavity is formed between the inner wall of the limiting ring and the outer wall of the bottle mouth, an oil collection shell is arranged on the outer wall of one side of the bottle body, a conveying pump is arranged at the top of the oil collection shell, an extension pipe is arranged on one side of the conveying pump, the other end of the extension. When the oil seal type oil collection device is used, the oil seal cavity ensures the air tightness between the installation ring and the bottle body, and when the installation ring is disassembled, oil in the oil seal cavity is pumped into the oil collection shell through the delivery pump and the extension pipe, so that the cyclic utilization is realized.

Description

Algae aeration culture device
Technical Field
The invention relates to the technical field of algae culture, in particular to an algae aeration culture device.
Background
Algae can be composed of one or a few cells, and many cells aggregate into a tissue-like architecture. The filaments may or may not be branched, some of the algae being unicellular dinoflagellates, others aggregating into populations. The pinus of green algae is formed by interweaving and winding countless branch filaments, the shapes and functions of the filaments at different positions are different, and when most kinds of algae are cultured, good ventilation needs to be ensured, and aseptic air tightness is effectively ensured.
Through search, the Chinese patent with publication number CN205954009U discloses an algae aeration culture device, which comprises a culture bottle for loading algae culture solution; the upper cover is used for being combined with the culture bottle, the top surface of the upper cover is provided with an opening and an air-tight plug arranged between the upper cover and the culture bottle, and the air-tight plug is used for forming a closed culture environment in the culture bottle; for connection to the intake pipe of an air pump. The aeration culture device for algae in the above patent has the following disadvantages: when in use, the air tightness of the bottle mouth is not good enough.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides an algae aeration culture device.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides an algae culture apparatus of ventilating, includes the bottle, and the top of bottle is provided with the bottle neck, and the circumference outer wall of bottle neck is provided with the screw thread, the circumference department threaded connection of bottle neck has the retainer ring, and the circumference inner wall of retainer ring is equipped with two rubber stoppers, two peg graft at the top of rubber stopper has outlet duct and intake pipe, and the circumference department of bottle top outer wall is equipped with the spacing collar, forms the oil blanket chamber between the inner wall of spacing collar and the outer wall of bottle neck, bottle one side outer wall is equipped with the oil-collecting shell, and the top of oil-collecting shell is equipped with the delivery pump, and one side of delivery pump is equipped with the extension pipe, and the other end of extension pipe extends to the.
As a still further scheme of the invention: the circumference outer wall of installing ring is equipped with the apron, and the external diameter of apron and the external diameter looks adaptation of spacing collar.
As a still further scheme of the invention: one side of the outer wall of the top of the bottle body is provided with a groove, and one end, far away from the delivery pump, of the extension pipe extends to the inside of the groove.
As a still further scheme of the invention: a lifting funnel is placed in the bottle body, and the bottom of the air inlet pipe extends to the bottom of the lifting funnel.
As a still further scheme of the invention: the top of lifting funnel circumference outer wall is the arc structure, and the top of lifting funnel circumference outer wall is provided with the cavity.
As a still further scheme of the invention: the intermediate position of lift funnel circumference outer wall is equipped with evenly distributed's connecting block, and the other end of connecting block is equipped with the direction funnel.
As a still further scheme of the invention: the cross section of guide funnel one side is L type structure, and leaves the clearance between the bottom of guide funnel's open-top inner wall and lift funnel circumference outer wall.
As a still further scheme of the invention: the both sides of lift funnel circumference inner wall all are equipped with the arc gag lever post, and the external diameter looks adaptation of the internal diameter of two arc gag lever posts and intake pipe.
As a still further scheme of the invention: one end of the air inlet pipe and one end of the air outlet pipe extending to the top of the bottle body are both provided with one-way valves.
As a still further scheme of the invention: the circumference department of lift funnel bottom is equipped with evenly distributed's connecting rod, and the bottom of connecting rod is equipped with the otter board.
The invention has the beneficial effects that:
1. the conveying pump, the limiting ring and the oil seal cavity are arranged, oil is filled into the oil seal cavity through the conveying pump, the mounting ring is fixed at the top of the bottle body in a threaded mode, the oil seal cavity ensures the air tightness between the mounting ring and the bottle body, and when the mounting ring is disassembled, the oil in the oil seal cavity is pumped into the oil collecting shell through the conveying pump and the extension pipe, so that cyclic utilization is achieved;
2. by arranging the guide funnel, the lifting funnel and the cavity, air injected from the bottom of the air inlet pipe forms bubbles, the guide funnel conducts limiting guide on the bubbles to the cavity, so that the lifting funnel floats upwards due to buoyancy at the cavity, and after the air is injected, the lifting funnel sinks downwards due to gravity, so that slow stirring of nutrient solution in the bottle body is realized, and the uniformity of the nutrient solution is ensured;
3. through setting up connecting rod and otter board, the lift funnel drives the otter board and goes up and down thereupon when going up and down, and the otter board drives the material of deposiing at the bottle bottom inner wall, guarantees the nutrient solution mixing effect.
Drawings
FIG. 1 is a schematic view showing the structure of an algal aeration culture apparatus as set forth in example 1;
FIG. 2 is a cross-sectional side view of the installation ring and the spacing ring of the aeration culture device for algae proposed in example 1;
FIG. 3 is a schematic view showing the overall structure of an algal aeration culture apparatus according to example 1;
FIG. 4 is a sectional view showing the side structure of the elevating funnel of the aeration-cultivation apparatus for algae proposed in example 2.
In the figure: the device comprises a bottle body 1, a screen plate 2, a connecting rod 3, an air inlet pipe 4, a connecting block 5, an arc limiting rod 6, an oil collecting shell 7, a delivery pump 8, an extension pipe 9, a groove 10, a cover plate 11, a rubber plug 12, an air outlet pipe 13, an installation ring 14, a limiting ring 15, an oil seal cavity 16, a lifting funnel 17, a cavity 18 and a guide funnel 19.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.
In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.
In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.
Example 1
Referring to fig. 1-3, an algae aeration culture apparatus comprises a bottle body 1, a bottle mouth is arranged at the top of the bottle body 1, threads are arranged on the circumferential outer wall of the bottle mouth, a mounting ring 14 is in threaded connection with the circumferential position of the bottle mouth, the circumferential inner wall of the mounting ring 14 is connected with two rubber plugs 12 through fastening bolts, an air outlet pipe 13 and an air inlet pipe 4 are inserted into the tops of the two rubber plugs 12, a limiting ring 15 is connected with the circumferential position of the outer wall of the top of the bottle body 1 through fastening bolts, an oil seal cavity 16 is formed between the inner wall of the limiting ring 15 and the outer wall of the bottle mouth, an oil collection shell 7 is connected with the outer wall of one side of the bottle body 1 through fastening bolts, a delivery pump 8 is connected with the top of the oil collection shell 7 through fastening bolts, an extension pipe 9 is connected with one side of the delivery pump 8 through fastening bolts, the other end of the, with the top of installing circle 14 threaded fixation at bottle 1, the gas tightness between installing circle 14 and the bottle 1 is guaranteed in oil blanket chamber 16, when dismantling installing circle 14, takes out the oil in oil blanket chamber 16 to the collection oil shell 7 in through delivery pump 8 and extension pipe 9, realizes cyclic utilization, injects the air into bottle 1 through intake pipe 4 to discharge unnecessary air through outlet duct 13, guarantee to ventilate.
Wherein, the circumference outer wall of installing ring 14 is connected with apron 11 through fastening bolt, and the external diameter looks adaptation of the external diameter of apron 11 and spacing collar 15, and during the installation installing ring 14, apron 11 covers the top in oil blanket chamber 16 sealedly, avoids the oil in the oil blanket chamber 16 to spill over.
Wherein, one side of the outer wall of the top of the bottle body 1 is provided with a groove 10, and one end of the extension pipe 9 far away from the delivery pump 8 extends to the inside of the groove 10, and when oil in the oil seal cavity 16 is extracted through the extension pipe 9, the oil flows into the groove 10, so that the oil pumping is ensured to be clean and efficient.
Wherein, the lift funnel 17 has been placed to the inside of bottle 1, and the bottom of intake pipe 4 extends to the bottom of lift funnel 17, and the top of the outer wall of 17 circumferences of lift funnel is the arc structure, and the top of the outer wall of 17 circumferences of lift funnel is provided with cavity 18.
Wherein, the middle position of the circumference outer wall of the lifting funnel 17 is connected with connecting blocks 5 which are evenly distributed through fastening bolts, and the other end of the connecting blocks 5 is connected with a guide funnel 19 through fastening bolts.
Wherein, the cross section of guide funnel 19 one side is L type structure, and leave the clearance between the open-top inner wall of guide funnel 19 and the bottom of lift funnel 17 circumference outer wall, the air that 4 bottoms of intake pipe injected forms the bubble, guide funnel 19 carries out spacing direction to the bubble to the cavity 18 in, make lift funnel 17 upwards float because of the buoyancy of cavity 18 department, after the completion of injecting the air, lift funnel 17 subsides downwards because of gravity, thereby realize the slow stirring to nutrient solution in the bottle 1, guarantee the nutrient solution homogeneity.
Wherein, the both sides of 17 circumference inner walls of lift funnel are all connected with arc gag lever post 6 through fastening bolt, and the internal diameter of two arc gag lever posts 6 and the external diameter looks adaptation of intake pipe 4, when lift funnel 17 goes up and down, arc gag lever post 6 carries out spacing direction to lift funnel 17, avoid lift funnel 17 and 1 inner wall of bottle to take place to collide with, and when dismantling collar 14, the accessible rotates intake pipe 4, make the bottom of intake pipe 4 stagger with two arc gag lever posts 6, the dismantlement of 14 and intake pipe 4 of collar of easy to assemble, convenient easy to operate.
Wherein, the one end that intake pipe 4 and outlet duct 13 extend to the bottle 1 top all is connected with the check valve through fastening bolt.
The working principle is as follows: oil is injected into the oil seal cavity 16 through the delivery pump 8, the mounting ring 14 is fixed at the top of the bottle body 1 in a threaded manner, the oil seal cavity 16 ensures the air tightness between the mounting ring 14 and the bottle body 1, when the mounting ring 14 is disassembled, oil in the oil seal cavity 16 is pumped into the oil collecting shell 7 through the delivery pump 8 and the extension pipe 9, the cyclic utilization is realized, air is injected into the bottle body 1 through the air inlet pipe 4, redundant air is discharged through the air outlet pipe 13, the ventilation is ensured, when the mounting ring 14 is mounted, the cover plate 11 covers and seals the top of the oil seal cavity 16, the oil in the oil seal cavity 16 is prevented from overflowing, when the oil in the oil seal cavity 16 is pumped through the extension pipe 9, the oil flows into the groove 10, the oil pumping is ensured to be clean and efficient, the air injected into the bottom of the air inlet pipe 4 forms bubbles, the guide funnel 19 conducts limiting and guiding on the bubbles into the cavity 18, after the completion of the injected air, lifting funnel 17 subsides downwards because of gravity, thereby realize the slow stirring to the nutrient solution in bottle 1, guarantee the nutrient solution homogeneous, when lifting funnel 17 goes up and down, arc gag lever post 6 carries out spacing direction to lifting funnel 17, avoid lifting funnel 17 to take place to collide with 1 inner wall of bottle, and when dismantling installation ring 14, the accessible rotates intake pipe 4, make the bottom of intake pipe 4 stagger with two arc gag lever posts 6, the dismantlement of easy-to-assemble ring 14 and intake pipe 4, convenient easy operation.
Example 2
Referring to fig. 4, in this embodiment, compared with embodiment 1, the connecting rods 3 are uniformly distributed at the circumference of the bottom of the lifting funnel 17 and connected by fastening bolts, and the mesh plate 2 is connected to the bottom of the connecting rods 3 by fastening bolts.
The working principle is as follows: the lifting funnel 17 drives the screen plate 2 to lift along with the lifting funnel, and the screen plate 2 drives the substances precipitated on the inner wall of the bottom of the bottle body 1, so that the mixing effect of the nutrient solution is ensured.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (10)

1. An algae aeration culture device comprises a bottle body (1), a bottle mouth is arranged at the top of the bottle body (1), threads are arranged on the circumferential outer wall of the bottle mouth, it is characterized in that the circumference of the bottle mouth is in threaded connection with a mounting ring (14), the circumferential inner wall of the mounting ring (14) is provided with two rubber plugs (12), the tops of the two rubber plugs (12) are inserted with an air outlet pipe (13) and an air inlet pipe (4), a limiting ring (15) is arranged at the circumference of the outer wall of the top of the bottle body (1), an oil seal cavity (16) is formed between the inner wall of the limiting ring (15) and the outer wall of the bottle mouth, an oil collection shell (7) is arranged on the outer wall of one side of the bottle body (1), a delivery pump (8) is arranged at the top of the oil collecting shell (7), an extension pipe (9) is arranged at one side of the delivery pump (8), and the other end of the extension pipe (9) extends to the inside of the limiting ring (15).
2. The aeration culture device for algae according to claim 1, wherein the mounting ring (14) is provided with a cover plate (11) on the outer circumferential wall, and the outer diameter of the cover plate (11) is matched with the outer diameter of the limiting ring (15).
3. An aerobic algae cultivation device according to any one of claims 1-2, wherein a groove (10) is formed in one side of the outer wall of the top of the bottle body (1), and the end of the extension pipe (9) away from the delivery pump (8) extends to the inside of the groove (10).
4. An aerobic algae cultivation device according to claim 3, wherein a lifting funnel (17) is placed inside the bottle body (1) and the bottom of the air inlet pipe (4) extends to the bottom of the lifting funnel (17).
5. The aeration cultivation device for algae according to the claim 4, characterized in that the top of the outer circumferential wall of the lifting funnel (17) is arc-shaped, and the top of the outer circumferential wall of the lifting funnel (17) is provided with a cavity (18).
6. An aeration cultivation device for algae according to claim 5, characterized in that the lifting funnel (17) is provided with evenly distributed connection blocks (5) at the middle position of the circumferential outer wall, and the other end of the connection blocks (5) is provided with a guide funnel (19).
7. The aeration cultivation device for algae according to claim 6, wherein the cross section of one side of the guide funnel (19) is L-shaped, and a gap is left between the inner wall of the top opening of the guide funnel (19) and the bottom of the outer circumferential wall of the lifting funnel (17).
8. The aeration cultivation device for algae according to claim 7, wherein the two sides of the inner wall of the circumference of the lifting funnel (17) are provided with arc-shaped limiting rods (6), and the inner diameters of the two arc-shaped limiting rods (6) are matched with the outer diameter of the air inlet pipe (4).
9. The aeration culture device for algae according to claim 8, wherein one end of the air inlet pipe (4) and one end of the air outlet pipe (13) extending to the top of the bottle body (1) are provided with one-way valves.
10. The aeration cultivation device for algae according to claim 9, wherein the lifting funnel (17) is provided with evenly distributed connecting rods (3) at the bottom circumference, and the bottom of the connecting rods (3) is provided with a mesh plate (2).
CN202010171366.0A 2020-03-12 2020-03-12 Algae aeration culture device Active CN111349544B (en)

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001129575A (en) * 1999-11-04 2001-05-15 Mitamura Setsubi Sekkei Jimusho:Kk Microorganism cultivating apparatus for treating polluted water
US20070091716A1 (en) * 2005-10-26 2007-04-26 Zeikus J G Pneumatic bioreactor
CN102021115A (en) * 2009-09-21 2011-04-20 惠识瑶 Packed bed type cell bioreactor without stirrer and method for cultivating animal cells
CN205954009U (en) * 2016-07-29 2017-02-15 聿新生物科技股份有限公司 Algae aeration culture device
CN208151392U (en) * 2018-03-29 2018-11-27 江西省科星生物工程有限公司 A kind of novel culture bottle
CN208485058U (en) * 2018-07-03 2019-02-12 湖北思搏盈环保科技有限公司 A kind of lye storage device of desulphurization denitration good airproof performance
CN208933388U (en) * 2018-08-03 2019-06-04 博瑞森(天津)生物科技有限公司 A kind of suspending cell culture bottle
CN209115817U (en) * 2018-08-29 2019-07-16 射洪县平安制氧有限责任公司 A kind of sealing device for oxygen production
CN209231159U (en) * 2018-10-24 2019-08-09 大唐华银攸县能源有限公司 One kind being used for thermal power plant's coal powder density on-line measurement device

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001129575A (en) * 1999-11-04 2001-05-15 Mitamura Setsubi Sekkei Jimusho:Kk Microorganism cultivating apparatus for treating polluted water
US20070091716A1 (en) * 2005-10-26 2007-04-26 Zeikus J G Pneumatic bioreactor
CN102021115A (en) * 2009-09-21 2011-04-20 惠识瑶 Packed bed type cell bioreactor without stirrer and method for cultivating animal cells
CN205954009U (en) * 2016-07-29 2017-02-15 聿新生物科技股份有限公司 Algae aeration culture device
CN208151392U (en) * 2018-03-29 2018-11-27 江西省科星生物工程有限公司 A kind of novel culture bottle
CN208485058U (en) * 2018-07-03 2019-02-12 湖北思搏盈环保科技有限公司 A kind of lye storage device of desulphurization denitration good airproof performance
CN208933388U (en) * 2018-08-03 2019-06-04 博瑞森(天津)生物科技有限公司 A kind of suspending cell culture bottle
CN209115817U (en) * 2018-08-29 2019-07-16 射洪县平安制氧有限责任公司 A kind of sealing device for oxygen production
CN209231159U (en) * 2018-10-24 2019-08-09 大唐华银攸县能源有限公司 One kind being used for thermal power plant's coal powder density on-line measurement device

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