CN111346683A - Pipetting device and pipetting method - Google Patents
Pipetting device and pipetting method Download PDFInfo
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- CN111346683A CN111346683A CN202010287059.9A CN202010287059A CN111346683A CN 111346683 A CN111346683 A CN 111346683A CN 202010287059 A CN202010287059 A CN 202010287059A CN 111346683 A CN111346683 A CN 111346683A
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- cavity
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0227—Details of motor drive means
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0237—Details of electronic control, e.g. relating to user interface
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Human Computer Interaction (AREA)
- Magnetically Actuated Valves (AREA)
Abstract
The invention discloses a liquid transfer device and a liquid transfer method. The pipetting device comprises a container with a cavity; the cavity is divided into a first cavity and a second cavity adjacent to the first cavity by a sealing valve; the inner wall of the container is provided with a valve seat for limiting the lowest stroke position of the sealing valve; a power mechanism is arranged in the first cavity and controls the sealing valve to move longitudinally relative to the power mechanism; and a flow passage for communicating the first cavity and the second cavity when the sealing valve leaves the valve seat is arranged between the sealing valve or the inner wall of the cavity and the sealing valve. The invention solves the problems of precision control and abrasion of the piston mechanical structure in the prior art through the change of the pressure difference, and trace liquid taking is completed through the micro-stroke of the movement of the sealing valve.
Description
Technical Field
The invention relates to a liquid transferring device, in particular to a liquid transferring device and a liquid transferring method.
Background
At present, an air displacement type pipettor such as a piston pipette structure needs to precisely position the position of a piston through a mechanical structure so as to meet the requirement of precise pipetting. However, due to inherent limitations of mechanical structures, especially for small liquid transfer amounts, such as 1 μ L liquid transfer amount, the positioning accuracy of the piston needs to be in the order of several micrometers, calculated within 5 mm of the inner diameter of the piston sleeve of the currently available pipettor and 5% of the accuracy, which puts extremely high demands on the angular resolution of the micro stepping or servo motor used for the pipettor, the machining accuracy and backlash of the transmission mechanism, such as a gear or a lead screw, and the backlash of various bearings.
In addition, accurate piston positioning can be realized only by good piston sealing performance. In order to avoid polluting reagents, the piston cannot be lubricated, and due to size limitation, the power of the motor cannot be very high, so that the interference magnitude of the piston cannot be designed to be too large, but the total service life of the instrument needs the piston to be well sealed after being formed by running for millions of times, and the requirements on the material of a sealing ring, the roughness of the machining of the inner wall of the piston sleeve and the like are high.
Disclosure of Invention
The purpose of the invention is as follows: in order to overcome the defects of the prior art, the invention provides a liquid transfer device, which solves the problems of high cost and difficult process of a mechanism for positioning by adopting piston motion in the prior art. The invention also provides a method for pipetting by using the pipetting device.
The technical scheme is as follows: the invention relates to a liquid transfer device, which comprises a container, a liquid storage container and a liquid transfer device, wherein the container is provided with a cavity;
the cavity is divided into a first cavity and a second cavity adjacent to the first cavity by a sealing valve; the inner wall of the container is provided with a valve seat for limiting the lowest stroke position of the sealing valve;
a power mechanism is arranged in the first cavity and controls the sealing valve to move relative to the power mechanism;
and a flow passage for communicating the first cavity and the second cavity when the sealing valve is separated from the valve seat is arranged on the sealing valve or between the inner wall of the cavity and the sealing valve.
The power mechanism is an electromagnetic spring and comprises an electromagnet mainly composed of an iron core and a coil; and a magnet structure is arranged on the end face of the sealing valve opposite to the power mechanism. Preferably, the magnet structure is a permanent magnet.
The sealing valve includes a valve core structurally fixed with the magnet.
The lower end surface of the valve core is protruded to form a circular truncated cone-shaped structure.
The valve seat includes a contact edge forming a sealing surface with the lower end surface of the valve element.
The valve seat is a separator formed by inward projection of the inner circumferential surface of the container; and/or the separating piece is a slope surface formed by inward projection of the inner circumferential surface of the container.
An opening at the lower end of the container is provided with a connector end for mounting a liquid-transfer gun head; and/or the interface end is detachably provided with a pipette tip.
The magnet structure of the sealing valve is embedded at the upper end of the valve core, and a gap capable of forming a flow channel is formed between the outer wall of the upper part of the sealing valve and the inner wall of the container.
The pipetting method of the pipetting device comprises the following steps:
(a) before pipetting: the sealing valve is attached to the valve seat, and a cavity in the container is divided into a first cavity and a second cavity, wherein the first cavity is in a stable negative pressure state, and the second cavity is in external air pressure in a use state; the sealing valve described in the present invention is attached to the valve seat, and in a preferred embodiment, the sealing valve is attached to the valve seat under the repulsive force of the power mechanism.
(b) When in pipetting: when liquid is to be sucked, the lower end part of the pipetting gun head arranged below the pipetting device is contacted with the liquid level, the sealing valve is separated from the valve seat due to stress change and communicated with the first cavity and the second cavity by reducing the repulsion force of the power mechanism to the sealing valve, the pressure in the second cavity is reduced, and the liquid is pressed into the pipetting gun head by external air pressure to take the liquid;
(c) and (6) discharging liquid.
Preferably, in the step (b), the sealing valve moves upwards to separate from the valve seat to communicate the first cavity and the second cavity, the pressure in the second cavity is reduced, and the liquid is pressed into the pipetting gun head arranged below the pipetting device by the atmospheric pressure to take the liquid.
Preferably, the step (b) is: when in pipetting: when liquid with the required liquid transfer amount is to be sucked, the lower end part of the liquid transfer gun head arranged below the liquid transfer device is made to contact with the liquid level, the repulsive force of the power mechanism to the sealing valve is reduced by controlling the current value of the coil on the power mechanism matched with the liquid transfer amount, the sealing valve moves upwards due to stress change and leaves the valve seat to communicate the first cavity and the second cavity, the pressure in the second cavity is reduced, and the liquid with the required liquid transfer amount is pressed into the liquid transfer gun head by external air pressure to take the liquid.
Preferably, the step (b) is: when in pipetting: when liquid with the required liquid transfer amount is to be sucked, the lower end part of the liquid transfer gun head arranged below the liquid transfer device is made to contact with the liquid level, the repulsive force of the power mechanism to the sealing valve is reduced by controlling the current value of the coil on the power mechanism adaptive to the liquid transfer amount, the sealing valve moves upwards along the longitudinal axis of the cavity due to stress change and leaves the valve seat to communicate the first cavity with the second cavity, the pressure in the second cavity is reduced, and the liquid with the required liquid transfer amount is sucked by the liquid transfer gun head arranged below the liquid transfer device to take the liquid.
Has the advantages that: (1) the invention overcomes the precision problem caused by mechanical transmission (such as piston motion) in the prior art, controls the movement of the sealing valve by controlling the stress change of the sealing valve through the power mechanism, and realizes liquid transfer through the pressure change in the upper cavity and the lower cavity of the sealing valve; (2) based on the structure and the structure provided by the invention, the repulsive force of the power mechanism to the sealing valve, which is adaptive to the liquid transfer amount, can be accurately adjusted by controlling the current value of the coil on the power mechanism, so that the movement of the sealing valve is controlled by the change of the stress on the upper surface and the lower surface of the sealing valve, and the accurate liquid transfer of the required liquid transfer amount is realized by the change of the pressure in the upper cavity and the lower cavity; (3) the invention further seals the side wall of the sealing valve with the valve seat, compared with the traditional side wall seal, the movement stroke is small, and the abrasion of the sealing surface of the sealing valve is effectively reduced; (4) according to the invention, the power mechanism is used for controlling the force difference at two sides of the sealing valve to control the movement of the sealing valve, so that the negative pressure in the first cavity and the pressure in the second cavity in the pipetting process do not need to be accurately controlled, only the negative pressure in the first cavity is stable before pipetting is prepared, and compared with the prior art, pressure sensors are not needed in the second cavity and the pipetting gun head, so that the structure is simple and ingenious, and the cost is saved; (5) the structure of the invention overcomes the problem that the liquid may volatilize to cause the pressure above the liquid level to increase and drip during the transferring process when the volatile liquid is transferred in the prior art, when the liquid transferring device provided by the invention is used for transferring liquid, the volatilization of the liquid can cause the pressure in the second cavity to rise, and then the sealing valve can be automatically jacked open to communicate the upper cavity and the lower cavity, thereby reducing the pressure in the second cavity and effectively preventing the liquid dropping during the transferring process, and the structure is reasonable without repeatedly moving the piston to compensate the increased pressure, and the usability is high.
Drawings
FIG. 1 is a schematic sectional view of the structure of embodiment 1 of the present invention;
fig. 2 is a schematic perspective view of the structure of one of the sealing valves used in embodiment 1 of the present invention;
fig. 3 is a schematic cross-sectional view of the sealing valve of fig. 2 when located in a container;
fig. 4 is a schematic sectional view showing a structure in which another sealing valve is used in embodiment 1 of the invention;
fig. 5 is a schematic perspective view of the structure of another sealing valve used in embodiment 1 of the present invention;
fig. 6 is a schematic cross-sectional view of the sealing valve of fig. 5 when located in a container.
Detailed Description
The structure of the invention is further explained in the following with the attached drawings.
Example 1: as shown in FIG. 1, the pipetting device of the invention comprises a container 10, wherein the container 10 is provided with a cavity 100, the container 10 is provided with a cavity 100 extending longitudinally in the embodiment, and the cavity is provided with a longitudinal axis 11.
The cavity 100 is partitioned into a first cavity 101 and a second cavity 102 located below the first cavity 101 by the sealing valve 20, when the pipetting device is equipped with the pipetting tip 60, the second cavity 102 includes a cavity surrounded by the pipetting tip 60, that is, the second cavity 102 is integrally communicated with the cavity of the pipetting tip 60, which is collectively referred to as the second cavity 102 in this embodiment, the second cavity 102 is communicated with the outside when no pipetting is performed, for example, when the pipetting device of this embodiment is used under a standard atmospheric pressure, the pressure of the second cavity 102 when no pipetting is performed is the standard atmospheric pressure.
The power mechanism 30 is disposed inside the first cavity 101, and the power mechanism 30 is configured to control a longitudinal distance (extending direction of the longitudinal axis 11) between the sealing valve 20 and the power mechanism 30, so that the sealing valve 20 is away from the valve seat 40, communicating the first cavity 101 and the second cavity 102, and in other embodiments, the sealing valve 20 is longitudinally movable along the longitudinal axis 11 relative to the power mechanism 30. In this embodiment, the power mechanism 30 is an electromagnetic spring, and the electromagnetic spring mainly includes an electromagnet composed of an iron core 301 and a coil 302 surrounding the iron core 301.
The inner wall of the container 10 is provided with a valve seat 40 for positioning and limiting the lowermost position of the sealing valve 20, and the valve seat 40 has a contact edge 401, and the contact edge 401 contacts with the side wall of the sealing valve 20, and constitutes a separation surface together with the sealing valve 20, dividing the chamber 100 into separate chambers (the first chamber 101 and the second chamber 102 in this embodiment).
The structure of the valve seat 40 forming the contact edge 401 can be various, such as a circular ring structure (torus) horizontally extending from the inner wall of the container 10, or a convex structure extending from the inner wall of the container 10, and the convex shape is not limited. When the contact edge 401 is formed by a ring surface, the ring surface may be arranged perpendicular to the longitudinal axis 11 or non-perpendicular to the longitudinal axis, that is, the contact edge 401 only needs to clamp the sealing valve 20 and form a structure for dividing the chamber 100 together with the sealing valve 20.
In order to control the sealing valve 20 by the power mechanism 30, an end surface of the sealing valve 20 opposite to the power mechanism 30 is a magnet structure 201 (permanent magnet), when the coil 302 is energized, a repulsive force is generated to the magnet structure, the sealing valve 20 is fixed on the valve seat 40 by the repulsive force, and the sealing valve 20 is clamped on the contact edge 401 of the valve seat 40.
Another structure of the sealing valve 20 in this embodiment is that the sealing valve 20 has a valve core 202 embedded with a magnet structure 201, a cavity for accommodating the magnet structure 201 is provided above the valve core 202, and the magnet structure 201 is fixed in the cavity, so that the magnet structure 201 and the valve core 202 form an integrated structure, and in order to further reduce the friction force between the sealing valve 20 and the valve seat 40 and not affect the sealing performance, the structure at the lower end of the sealing valve 20 is preferably a truncated cone-shaped structure, and the truncated cone-shaped surface of the truncated cone-shaped structure and the contact edge 401 form a sealing surface to separate the first cavity 101 from the second cavity 102.
In order to fit the truncated cone-shaped structure of the lower end of the sealing valve 20, a partition 402 formed by the valve seat 40 protruding inward on the inner circumferential surface of the container 10, that is, the sealing valve 20 is caught on the partition 402, and at the same time, a flow passage 103 for air circulation is provided between the upper portion of the sealing valve 20 and the inner wall of the container 10.
Further, the valve seat 40 is a slope surface (an inverted truncated cone-shaped structure matched with the truncated cone-shaped structure at the lower end of the sealing valve 20) formed by inward protrusion of the inner circumferential surface of the container 10, and when the structure is adopted, the structure of the container 10 can be optimized at the same time, that is, the inner diameter of the second cavity 102 is smaller than the inner diameter of the first cavity 101, and the joint of the first cavity 101 and the second cavity 102 forms a slope surface, which is the valve seat 40 structure described in this embodiment, and the truncated cone-shaped structure at the lower end of the sealing valve 20 is clamped on the slope surface, that is, the inverted truncated cone-shaped structure matched with the lower end of the sealing valve 20, and when the structure is adopted, the contact surface between the sealing valve 20 and the.
The structure of the sealing valve 20 is further optimized, that is, the sealing valve 20 is composed of a circular truncated cone structure and a cylindrical structure, the circular truncated cone structure is clamped on the slope surface, the outer diameter of the cylindrical structure (located in the first cavity) is smaller than the inner diameter of the first cavity, and a gap between the cylindrical structure and the inner wall of the first cavity 101 forms a flow passage 103; the valve seat 40 adopts a slope surface structure and a circular truncated cone structure of the sealing valve 20, so that the abrasion can be further reduced, and the service life is prolonged.
The flow passage 103 provided in the sealing valve 20 for communicating the first chamber 101 and the second chamber 102 when the sealing valve is away from the valve seat can also be realized in other ways. As shown in fig. 2, in some embodiments, the sealing valve 20 is composed of a circular truncated cone structure and a cylindrical structure located at the upper part, when the sealing valve 20 is attached to the valve seat 40, the bottom surface of the circular truncated cone structure partially fits on the slope surface, and the cylindrical structure of the sealing valve is recessed in the edge of the cylinder to form a plurality of circumferentially distributed flow channels 103, as shown in fig. 3, the horizontal cross section of the cylindrical structure above the sealing valve 20 is a circular surface with a plurality of small gaps circumferentially distributed on the outer edge, in this embodiment, the outer wall of the cylindrical structure partially fits on the inner wall of the first cavity, and due to the gap structure on the outer edge of the cross section, a gap capable of forming the flow channel 103 is formed between the outer wall of the cylindrical structure and the inner wall of the first cavity of the container, and when the sealing valve is away.
As shown in fig. 4 and 5, in some embodiments, the sealing valve 20 is composed of a circular truncated cone structure and a cylindrical structure located at the upper part, the bottom surface of the circular truncated cone structure partially fits on the slope surface when the sealing valve fits on the valve seat, and a plurality of circular through holes are circumferentially distributed on the circular surface of the cylindrical structure part of the sealing valve far away from the circle center and close to the outer edge, as shown in fig. 6.
Of course, the flow channel 103 may be implemented in other manners as long as the sealing valve can separate the first cavity 101 and the second cavity 102 by the sealing valve when the sealing valve is attached to the valve seat, and the first cavity 101 and the second cavity 102 are communicated by the flow channel 103 when the sealing valve is detached from the valve seat.
In this embodiment, the opening at the lower end of the container 10 is provided with a mouthpiece end 50 for mounting a pipette tip, the mouthpiece end 50 is detachably mounted with the pipette tip 60, and the connection mode between the mouthpiece end 50 and the pipette tip 60 can adopt any structure in the prior art, so that the pipette tip 60 can be detachably sleeved.
The liquid transfer method of the liquid transfer device comprises the following steps:
(a) before pipetting: the sealing valve 20 is attached to the valve seat 40 under the repulsive force of the power mechanism, and divides the cavity in the container 10 into a first cavity 101 and a second cavity 102, wherein the first cavity 101 is in a stable negative pressure state, and the second cavity 102 is external air pressure in a use state, in this embodiment, standard atmospheric pressure;
(b) when in pipetting: when liquid is to be sucked, the lower end part of the pipetting gun head arranged below the pipetting device is contacted with the liquid level, the repulsive force of the power mechanism 30 to the sealing valve 20 is reduced by controlling the current value of a coil on the power mechanism 30 matched with the pipetting quantity, so that the sealing valve 20 moves upwards along the longitudinal axis 11 and is separated from the valve seat 40 due to the change of stress (the pressure difference between the upper cavity and the lower cavity) to communicate the first cavity 101 and the second cavity 102, the pressure in the second cavity is reduced, and the liquid is pressed into the pipetting gun head arranged below the pipetting device by the atmospheric pressure to take the liquid;
(c) and (6) discharging liquid.
The working principle of the liquid-transfering device is as follows:
the first cavity 101 is filled with a stable negative pressure, the iron core 301 and the coil 302 form an electromagnet as a power mechanism, the power mechanism 30 is powered on when preparing to transfer liquid, the electromagnet presses the valve core 202 on the valve seat 40 inside the container 10 through the repulsion force to the magnet structure 20, and the valve core 202 and the valve seat 40 form a sealing surface together through the slope of the valve seat 40, so that the first cavity 101 is completely separated from the second cavity 102. When a specified volume of liquid needs to be sucked, the lower end of the pipette tip 60 is firstly contacted with the liquid level, then the repulsive force is reduced by the current of the control coil 302, the atmospheric pressure in the pipette tip 60 will push the push valve core 202 upwards along the longitudinal axis 11 for a small distance, so that the first cavity 101, the second cavity 102 and the inner cavity of the pipette tip of the container 10 are communicated through the flow channel 103 around the valve core 202, the pressure in the pipette tip 60 is reduced, the liquid is pressed into the pipette tip 60 by the atmospheric pressure, when the pressure difference between the cavity on the liquid column in the pipette tip (i.e. the second cavity 102) and the first cavity 101 of the container 10 is reduced to the sum of the electromagnetic repulsive force and the gravity force applied to the magnet, the sealing valve 20 is closed under the repulsive force of the magnet (when the pressure difference between the first cavity 101 and the second cavity 102 is balanced with the repulsive force of the power mechanism 30 and the gravity force of the sealing valve, the pressure in the first chamber 101 is no longer reduced). The parameters affecting the current are the dimensions of the valve element 202, the dimensions of the core 301 and the coil 302 and the container 10, which are known parameters available before pipetting, so that the pressure in the pipette tip 60 can be indirectly and accurately controlled by controlling the current in the coil 3, and the difference between the pressure in the pipette tip 60 and the atmospheric pressure determines the height of the column of liquid in the pipette tip 60, i.e. the amount of pipetted liquid (the amount of pipetted liquid may also be referred to as the pipetting amount).
The liquid discharging method of the liquid-transfering device of the invention can be realized by the prior art, and is not described in detail, for example, the liquid discharging method can be realized by increasing the pressure in the first cavity 101 (the pressure in the first cavity 101 is greater than the pressure in the second cavity 102, so as to prevent the liquid in the liquid-transfering gun head 60 from flowing backwards), then changing the current direction in the electromagnet coil 302, changing the repulsion force of the electromagnet on the upper end of the sealing valve 20 into the attraction force, separating the sealing valve 20 from the valve seat 40 to communicate the first cavity and the second cavity, and discharging the liquid in the liquid-transfering gun head 60 by the pressure of the first. Alternatively, a bypass (not shown) may be provided in the side wall of the mouthpiece end 50 to communicate with the second chamber 102, the bypass being closed during withdrawal and opened during withdrawal to allow gas to be fed into the second chamber 102 from the bypass to expel liquid from the pipette tip 60. In addition to the above-listed modes, the liquid-transferring device of the present invention can also realize liquid-discharging by adopting other technologies in the prior art.
It can be seen from the structure of the present invention that the opening and closing of the sealing valve 20 is controlled by an electromagnetic structure, and there is no trouble of mechanical positioning, and in addition, because the movement stroke of the sealing valve 20 in the open and closed state is very small, and the sealing of a non-vertical surface (such as an inverted frustum-shaped sealing contact surface) can be realized by the sealing valve 20 and the valve seat slope surface, the side wall of the sealing valve 20 contacting the container 10 is also preferably in clearance fit, so that the running wear of the sealing valve is greatly reduced, and the sealing valve is durable. The valve seat adopts a slope surface structure, so that the slope surface-slope surface sealing can be realized under the action of gravity compaction without interference fit, and the problem of abrasion caused by interference fit when the vertical side surface is sealed in the prior art is further solved.
In addition, the opening and closing of the sealing valve 20 are controlled by adjusting the repulsive force of the magnet, namely adjusting the difference between the bearing forces at the two ends of the valve seat 40, so that the negative pressure in the first cavity and the pressure in the second cavity in the pipetting process do not need to be accurately controlled, the negative pressure in the first cavity is stable only before pipetting is prepared, and compared with the prior art, pressure sensors do not need to be used in the second cavity and the pipetting gun head, so that the structure is more reasonable, and the pipetting gun is simplified and ingenious. If the liquid sucked is volatile, the pressure in the pipette tip 60 above the liquid level rises, the sealing valve 20 can be automatically opened to communicate the upper cavity and the lower cavity to reduce the pressure in the pipette tip above the liquid level, so that liquid dropping in the transfer process is prevented, the problem that the piston needs to be repeatedly moved to compensate the increased pressure in the prior art is effectively solved, and the pipette tip has better usability.
Claims (10)
1. Pipetting device, characterized by comprising a container (10), said container (10) having a cavity (100);
the cavity (100) is divided into a first cavity (101) and a second cavity (102) adjacent to the first cavity (101) by a sealing valve (20); the inner wall of the container (10) is provided with a valve seat (40) for limiting the lowest stroke position of the sealing valve (20);
a power mechanism (30) is arranged in the first cavity (101), and the power mechanism (30) controls the sealing valve (20) to move relative to the power mechanism (30);
and a flow passage (103) which is arranged on the sealing valve (20) or between the inner wall of the cavity (100) and the sealing valve (20) and is communicated with the first cavity (101) and the second cavity (102) when the sealing valve (20) is separated from the valve seat (40).
2. Pipetting device according to claim 1, characterized in that the power mechanism (30) is an electromagnetic spring comprising an electromagnet consisting essentially of an iron core (301) and a coil (302); and a magnet structure (201) is arranged on the end face of the sealing valve (20) opposite to the power mechanism (30).
3. Pipetting device according to claim 2, characterized in that the sealing valve (20) comprises a valve cartridge (202) fixed with the magnet arrangement (201).
4. Pipetting device according to claim 3, characterized in that the lower end face of the valve element (202) is raised in a truncated cone-shaped configuration.
5. Pipetting device according to claim 3, characterized in that the valve seat (40) comprises a contact edge (401) forming a sealing surface with the lower end face of the valve element (202).
6. Pipetting device according to claim 3, characterized in that the valve seat (40) is a partition (402) formed by an inward projection of the inner circumferential surface of the container (10); and/or the separator (402) is a slope surface formed by inward projection of the inner circumferential surface of the container (10).
7. Pipetting device according to claim 1, characterized in that the container (10) is open at its lower end provided with a mouthpiece (50) for mounting a pipette tip; and/or the interface end (50) is detachably provided with a liquid transfer gun head (60).
8. Pipetting device according to claim 3, characterized in that the magnet structure of the sealing valve (20) is embedded in the upper end of the valve cartridge (202), and a gap forming the flow channel (103) is provided between the outer wall of the upper part of the sealing valve (20) and the inner wall of the container (10).
9. A pipetting method for a pipetting device according to claim 1, characterized by comprising the steps of:
(a) before pipetting: the sealing valve (20) is attached to the valve seat (40) to divide a cavity (100) in the container (10) into a first cavity (101) and a second cavity (102), wherein the first cavity (101) is in a stable negative pressure state, and the second cavity (102) is in external air pressure in a use state;
(b) when in pipetting: when liquid is to be sucked, the lower end part of the pipetting gun head arranged below the pipetting device is contacted with the liquid level, the repulsion force of the power mechanism (30) to the sealing valve (20) is reduced, the sealing valve (20) is separated from the valve seat (40) due to stress change, the first cavity (101) and the second cavity (102) are communicated, the pressure in the second cavity (102) is reduced, and the liquid is pressed into the pipetting gun head by external air pressure to take the liquid;
(c) and (6) discharging liquid.
10. A pipetting method for a pipetting device according to claim 9, characterized in that the step (b) is: when in pipetting: when liquid with the required liquid transfer amount is to be sucked, the lower end part of the liquid transfer gun head arranged below the liquid transfer device is made to contact with the liquid level, then the current value of a coil on a power mechanism (30) matched with the liquid transfer amount is controlled, so that the repulsive force of the power mechanism (30) to a sealing valve (20) is reduced, the sealing valve (20) moves upwards due to stress change and is separated from a valve seat (40) to communicate a first cavity (101) and a second cavity (102), the pressure in the second cavity (102) is reduced, and the liquid with the required liquid transfer amount is pressed into the liquid transfer gun head by external air pressure to take the liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010287059.9A CN111346683A (en) | 2020-04-13 | 2020-04-13 | Pipetting device and pipetting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010287059.9A CN111346683A (en) | 2020-04-13 | 2020-04-13 | Pipetting device and pipetting method |
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| Publication Number | Publication Date |
|---|---|
| CN111346683A true CN111346683A (en) | 2020-06-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010287059.9A Pending CN111346683A (en) | 2020-04-13 | 2020-04-13 | Pipetting device and pipetting method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112729958A (en) * | 2021-01-11 | 2021-04-30 | 吉林大学 | Pipetting device and pipetting control method |
| CN113814012A (en) * | 2021-09-16 | 2021-12-21 | 上海陶术生物科技有限公司 | Reagent sample is with stabilizing liquid-transfering equipment |
| WO2022012562A1 (en) * | 2020-07-14 | 2022-01-20 | 广东润鹏生物技术有限公司 | Pipette, sample processing apparatus, and molecular diagnosis system |
-
2020
- 2020-04-13 CN CN202010287059.9A patent/CN111346683A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022012562A1 (en) * | 2020-07-14 | 2022-01-20 | 广东润鹏生物技术有限公司 | Pipette, sample processing apparatus, and molecular diagnosis system |
| CN112729958A (en) * | 2021-01-11 | 2021-04-30 | 吉林大学 | Pipetting device and pipetting control method |
| CN112729958B (en) * | 2021-01-11 | 2023-09-26 | 吉林大学 | Pipetting device and pipetting control method |
| CN113814012A (en) * | 2021-09-16 | 2021-12-21 | 上海陶术生物科技有限公司 | Reagent sample is with stabilizing liquid-transfering equipment |
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