CN111329492A - Noninvasive blood lipid detection device and detection method based on near infrared spectrum - Google Patents
Noninvasive blood lipid detection device and detection method based on near infrared spectrum Download PDFInfo
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Abstract
The invention discloses a noninvasive blood lipid detection device and a detection method based on near infrared spectrum, wherein the detection device comprises a broadband light source laser, an optical fiber beam splitter, a first focusing lens, a second focusing lens, a first optical fiber probe, a second optical fiber probe, a third focusing lens, a fourth focusing lens, a first near infrared spectrometer, a second near infrared spectrometer, a lock-in amplifier, a heart rate measuring instrument and a computer; according to the method, a relation curve of the blood lipid characteristic peak relative intensity of the near-infrared transmission spectrum and the blood lipid content is obtained by fitting a large amount of data through the relative value of the blood lipid characteristic peak at 2222nm to the hemoglobin characteristic peak at 2298nm in the human pure blood near-infrared transmission spectrum and the known blood lipid content of a human body, and then the real-time blood lipid content of the human body is obtained through detection. The invention has the advantages of real-time detection, rapidness, safety, environmental protection and the like, and has wide application prospect in the fields of biological non-invasive detection and disease prevention and treatment.
Description
Technical Field
The invention relates to the technical field of biological noninvasive detection, in particular to a noninvasive blood lipid detection device and a detection method based on near infrared spectrum.
Background
In recent years, with the rapid development of economy in China, the living standard of people is continuously improved, and the incidence of a series of diseases such as hyperlipidemia is remarkably increased due to unreasonable diet and living habits and environmental pollution. Hyperlipidemia patients can cause a series of complications due to hyperlipidemia, including a series of serious diseases such as vein sclerosis, coronary heart disease, hypertension, myocardial infarction, cerebral infarction, encephalatrophy and the like. Statistics shows that the number of people died of cardiovascular and cerebrovascular diseases in China is over 200 million, accounts for 40% of the number of the people died of the cardiovascular and cerebrovascular diseases, and is the first cause of death. Epidemiological statistics show that the coronary heart disease accounts for 10 to 20 percent of the death people caused by the heart disease in China; the mortality rate of coronary heart disease in developed countries exceeds the mortality rate of cancer, and in the aged 60-70 years old, the prevalence rate of hypertension is over 20%, and over 50% in the aged 70 years old. Therefore, cardiovascular and cerebrovascular diseases caused by hyperlipidemia are the main killers of human with high morbidity, high disability rate and high mortality, so the method is very important for real-time detection of the blood lipid.
The existing blood fat detection technology mainly adopts an invasive or minimally invasive method, for the method, a large amount of chemical reagents are often needed for detection, the environment is polluted, a large amount of time is consumed, and most importantly, the safety of a patient is possibly affected due to wound infection. At present, biochemical detectors are mostly adopted in the regular hospitals for detecting blood fat, although the method is high in precision, the detection time is long, the cost is high, and the real-time detection of the blood fat cannot be realized. The minimally invasive blood lipid detector in the market usually needs a patient to sample by himself to detect, and is convenient, but low in precision and reliability.
Disclosure of Invention
The invention aims to solve the technical problem of providing a near infrared spectrum-based noninvasive blood lipid detection device and a detection method, which have the advantages of real-time detection, rapidness, safety, environmental protection and the like, aiming at the defects of the prior art.
The technical scheme adopted by the invention for solving the technical problems is as follows: a noninvasive blood lipid detection device based on near infrared spectrum comprises a broadband light source laser, an optical fiber beam splitter, a first focusing lens, a second focusing lens, a first optical fiber probe, a second optical fiber probe, a third focusing lens, a fourth focusing lens, a first near infrared spectrometer, a second near infrared spectrometer, a lock-in amplifier, a heart rate measuring instrument and a computer, wherein the optical fiber beam splitter comprises an input end and two output ends, the output end of the broadband light source laser is connected with the input end of the optical fiber beam splitter through a first optical fiber, one output end of the optical fiber beam splitter is connected with the input end of the first focusing lens through a second optical fiber, the output end of the first focusing lens is connected with the input end of the first optical fiber probe through a third optical fiber, and the other output end of the optical fiber beam splitter is connected with the input end of the second focusing lens through a fourth optical fiber, the output end of the second focusing lens is connected with the input end of the second optical fiber probe through a fifth optical fiber, the first optical fiber probe and the second optical fiber probe are respectively used for collecting near infrared spectrum signals of a human body detection part, the output end of the first optical fiber probe is connected with the input end of the third focusing lens through a sixth optical fiber, the output end of the third focusing lens is connected with the signal input end of the first near infrared spectrometer, the signal output end of the first infrared spectrometer is connected with the signal input end of the computer, the output end of the second optical fiber probe is connected with the input end of the fourth focusing lens through a seventh optical fiber, the output end of the fourth focusing lens is connected with the signal input end of the second near infrared spectrometer, and the signal output end of the second infrared spectrometer is connected with the detection signal input end of the lock-in amplifier, the reference signal input end of the phase-locked amplifier is connected with the signal output end of the heart rate measuring instrument, the heart rate measuring instrument is used for measuring the heart rate of a human body, the amplification signal output end of the phase-locked amplifier is connected with the signal input end of the computer, the trigger signal output end of the computer is connected with the signal input end of the broadband light source laser, and the first near-infrared spectrometer and the second near-infrared spectrometer are respectively used for collecting near-infrared spectrum signals and splitting light to obtain spectrum data and convert the light signals into electric signals.
Preferably, the first optical fiber probe and the second optical fiber probe have the same structure and both comprise a hollow circular truncated cone body and a fifth focusing lens, the hollow circular truncated cone body is an opaque hollow circular truncated cone body, the fifth focusing lens is embedded in a central hole of the hollow circular truncated cone body, the distance between the front end face of the fifth focusing lens and the front end face of the hollow circular truncated cone body is 2-5 mm, a connecting optical fiber is arranged at the rear end of the central hole of the hollow circular truncated cone body in a penetrating manner, the connecting optical fiber is opposite to the rear end of the fifth focusing lens, and the connecting optical fibers in the first optical fiber probe and the second optical fiber probe are respectively connected with the third optical fiber and the fifth optical fiber. The hollow cone has a positioning effect on the fifth focusing lens, and can shield external interference light, so that the signal-to-noise ratio is improved. The fifth focusing lens can improve the convergence of light beams, thereby improving the energy density and the utilization rate of the light beams and improving the signal to noise ratio.
Preferably, the first focusing lens, the second focusing lens, the third focusing lens, the fourth focusing lens and the fifth focusing lens are all broadband achromatic lenses, the numerical apertures of the broadband achromatic lenses are all 0.25, and the focal lengths of the broadband achromatic lenses are all 4 mm.
Preferably, the first optical fiber, the second optical fiber, the third optical fiber, the fourth optical fiber, the fifth optical fiber, the sixth optical fiber, the seventh optical fiber and the connecting optical fiber are all quartz single-mode optical fibers.
Preferably, the broadband light source emitted by the output end of the broadband light source laser is supercontinuum laser, the wavelength range of the laser is 600 nm-2500 nm, and the power of the laser is 500 mv.
Preferably, the optical fiber used by the optical fiber beam splitter is a quartz optical fiber, the transmittance of the quartz optical fiber is more than 98%, the splitting ratio is 50:50, and the deviation of the splitting ratio is +/-4%.
Preferably, the measurement ranges of the first near-infrared spectrometer and the second near-infrared spectrometer are both 500-2500 nm, and the resolutions are both better than 0.5 nm.
Preferably, the measurement range of the heart rate measuring instrument is 40-200 pulse times/minute, and the measurement error is +/-5%.
Preferably, the human body detection part is the middle part of a thumb nail of a human hand.
The non-invasive blood fat detection method implemented by the non-invasive blood fat detection device comprises the following steps:
(1) selecting a plurality of volunteers with different blood lipid contents, extracting blood of the volunteers, and testing and recording the blood lipid content of each volunteer by using a blood lipid biochemical analyzer;
(2) measuring the near-infrared transmission spectrum blood fat characteristic peak of each volunteer, wherein the measuring process comprises the following steps:
(2-1) under the condition that the external environment condition is stable and appropriate, collecting the heart rate of the human body in real time by using a heart rate measuring instrument and inputting a heart rate signal into a reference signal input end of a phase-locked amplifier; selecting a human body detection part, respectively fixing a first optical fiber probe and a second optical fiber probe on the same human body detection part, and respectively contacting the first optical fiber probe and the second optical fiber probe with the same human body detection part;
(2-2) triggering a broadband light source laser, enabling laser to enter an optical fiber beam splitter through a first optical fiber for splitting to obtain two beams, namely a first beam and a second beam, taking the first beam as reference light and the second beam as signal light, enabling the first beam to enter a first focusing lens through a second optical fiber, then enabling the first beam to enter a first optical fiber probe through a third optical fiber, irradiating the first optical fiber probe to a human body detection part, obtaining a near infrared spectrum signal after blood scattering, enabling the near infrared spectrum signal to enter a signal input end of a first near infrared spectrometer after entering a third focusing lens through a sixth optical fiber; after entering a second focusing lens through a fourth optical fiber, a light beam enters a second optical fiber probe through a fifth optical fiber, irradiates a human body detection part through the second optical fiber probe, is scattered by blood to obtain a near infrared spectrum signal, and enters a signal input end of a second near infrared spectrometer after entering a fourth focusing lens through a seventh optical fiber;
(2-3) directly inputting an output signal of the first near-infrared spectrometer into a signal input end of a computer, and processing by the computer to obtain a reference spectrum L10; the output signal of the second near-infrared spectrometer enters the detection signal input end of the lock-in amplifier, meanwhile, the heart rate signal of the human body is used as a reference signal and is input to the lock-in amplifier through the reference signal input end, the lock-in amplifier amplifies the near-infrared light signal in the blood, the spectrum information in the blood is locked, then the spectrum information is input to the signal input end of the computer in a signal form, and the signal spectrum L20 is obtained through the processing of the computer;
(2-4) normalizing the reference spectrum L10 by using a computer with a hemoglobin characteristic peak at 2298nm to obtain a spectrum L1; for the signal spectrum L20, normalizing by a computer by using a hemoglobin characteristic peak at 2298nm to obtain a spectrum L2; subtracting the spectrum L1 from the spectrum L2, and dividing the spectrum L1 by the spectrum L0 of the broadband light source emitted by the output end of the broadband light source laser to obtain a near-infrared transmission spectrum T in the pure blood, namely T (L2-L1)/L0;
(2-5) carrying out normalization processing on the hemoglobin characteristic peak at 2298nm by using the blood lipid characteristic peak at 2222nm in the near-infrared transmission spectrum T by using a computer to obtain the relative intensity of the blood lipid characteristic peak of the human body near-infrared transmission spectrum;
(3) according to the relative intensities of the blood lipid characteristic peaks and the blood lipid contents of the near-infrared transmission spectra of different volunteers, fitting and establishing a relation curve of the relative intensities of the blood lipid characteristic peaks and the blood lipid contents of the near-infrared transmission spectra by using a least square method;
(4) and (3) for the person to be detected needing noninvasive blood lipid detection, carrying out measurement in the steps (2-1) to (2-5) once to obtain the actually measured near-infrared transmission spectrum blood lipid characteristic peak relative intensity of the person to be detected, and substituting the actually measured near-infrared transmission spectrum blood lipid characteristic peak relative intensity into the relation curve established in the step (3) to obtain the real-time blood lipid content of the person to be detected.
Compared with the prior art, the invention has the following advantages: the invention utilizes rich human body component information contained in the near infrared spectrum to realize the noninvasive detection of human body blood fat based on the near infrared spectrum. According to the method, a relation curve of the blood lipid characteristic peak relative intensity of the near-infrared transmission spectrum and the blood lipid content is obtained by fitting a large amount of data through the relative value of the blood lipid characteristic peak at 2222nm to the hemoglobin characteristic peak at 2298nm in the human pure blood near-infrared transmission spectrum and the known blood lipid content of a human body, and then the real-time blood lipid content of the human body is obtained through detection. The invention has the advantages of real-time detection, rapidness, safety, environmental protection and the like, and has wide application prospect in the fields of biological non-invasive detection and disease prevention and treatment.
Drawings
FIG. 1 is a structural connection frame of the non-invasive blood lipid detection device of the present invention;
FIG. 2 is an external view of a first fiber-optic probe or a second fiber-optic probe according to example 2;
FIG. 3 is a structural sectional view of a first fiber-optic probe or a second fiber-optic probe in embodiment 2;
FIG. 4 is a graph showing the relationship between the relative intensity of the characteristic peak of blood lipid and the content of blood lipid in the near infrared transmission spectrum in example 3.
Detailed Description
The invention is described in further detail below with reference to the accompanying examples.
Example 1: a noninvasive blood lipid detection device based on near infrared spectrum is disclosed, as shown in figure 1, and comprises a broadband light source laser 1, an optical fiber beam splitter 3, a first focusing lens 6, a second focusing lens 7, a first optical fiber probe 10, a second optical fiber probe 11, a third focusing lens 14, a fourth focusing lens 15, a first near infrared spectrometer 16, a second near infrared spectrometer 17, a lock-in amplifier 18, a heart rate measuring instrument 20 and a computer 19, wherein the optical fiber beam splitter 3 comprises an input end and two output ends, the output end of the broadband light source laser 1 is connected with the input end of the optical fiber beam splitter 3 through a first optical fiber 2, one output end of the optical fiber beam splitter 3 is connected with the input end of the first focusing lens 6 through a second optical fiber 4, the output end of the first focusing lens 6 is connected with the input end of the first optical fiber probe 10 through a third optical fiber 8, the other output end of the optical fiber beam splitter 3 is connected with the input end of the second focusing lens 7 through a fourth optical fiber 5, the output end of the second focusing lens 7 is connected with the input end of a second optical fiber probe 11 through a fifth optical fiber 9, the first optical fiber probe 10 and the second optical fiber probe 11 are respectively used for collecting near infrared spectrum signals of a human body detection part, the output end of the first optical fiber probe 10 is connected with the input end of a third focusing lens 14 through a sixth optical fiber 12, the output end of the third focusing lens 14 is connected with the signal input end of a first near infrared spectrometer 16, the signal output end of the first infrared spectrometer is connected with the signal input end of a computer 19, the output end of the second optical fiber probe 11 is connected with the input end of a fourth focusing lens 15 through a seventh optical fiber 13, the output end of the fourth focusing lens 15 is connected with the signal input end of a second near infrared spectrometer 17, the signal output end of the second infrared spectrometer is connected with the detection signal input end of a phase-locked amplifier 18, the reference signal input end of the phase-locked amplifier 18 is connected with the signal, the heart rate measuring instrument 20 is used for measuring the heart rate of a human body, the amplified signal output end of the lock-in amplifier 18 is connected with the signal input end of the computer 19, the trigger signal output end of the computer 19 is connected with the signal input end of the broadband light source laser 1, and the first near-infrared spectrometer 16 and the second near-infrared spectrometer 17 are respectively used for collecting near-infrared spectrum signals and splitting light to obtain spectrum data and converting the light signals into electric signals.
In embodiment 1, the broadband light source emitted by the output end of the broadband light source laser 1 is a supercontinuum laser, the wavelength range of the laser is 600nm to 2500nm, and the power of the laser is 500 mv; the optical fiber used by the optical fiber beam splitter 3 is a quartz optical fiber, the transmittance of the optical fiber is more than 98 percent, the splitting ratio is 50:50, and the deviation of the splitting ratio is +/-4 percent; the measurement ranges of the first near-infrared spectrometer 16 and the second near-infrared spectrometer 17 are both 500-2500 nm, and the resolutions are both better than 0.5 nm; the measurement range of the heart rate measuring instrument 20 is 40-200 pulse times/minute, and the measurement error is +/-5%.
The noninvasive blood lipid measuring device based on near infrared spectroscopy of example 2 is different from example 1 in that, in example 2, as shown in fig. 2 and 3, the first optical fiber probe 10 and the second optical fiber probe 11 have the same structure, and both include a hollow circular truncated cone 101 and a fifth focusing lens 102, the hollow circular truncated cone 101 is an opaque hollow circular truncated cone 101, the fifth focusing lens 102 is embedded in a central hole 103 of the hollow circular truncated cone 101, a distance between a front end face of the fifth focusing lens 102 and a front end face of the hollow circular truncated cone 101 is 3mm, a connecting optical fiber 104 is inserted through a rear end of the central hole 103 of the hollow circular truncated cone 101, the connecting optical fiber 104 faces a rear end of the fifth focusing lens 102, a protective sleeve 105 is disposed outside the connecting optical fiber 104, and the connecting optical fiber 104 in the first optical fiber probe 10 and the second optical fiber probe 11 is connected to the third optical fiber 8 and the fifth optical fiber 9, respectively.
In embodiment 2, the first focusing lens 6, the second focusing lens 7, the third focusing lens 14, the fourth focusing lens 15 and the fifth focusing lens 102 are all broadband achromatic lenses, the numerical apertures thereof are all 0.25, and the focal lengths thereof are all 4 mm; the first optical fiber 2, the second optical fiber 4, the third optical fiber 8, the fourth optical fiber 5, the fifth optical fiber 9, the sixth optical fiber 12, the seventh optical fiber 13 and the connecting optical fiber 104 are all quartz single-mode optical fibers.
Example 3: the noninvasive blood lipid detection method implemented by the noninvasive blood lipid detection device of embodiment 2 includes the following steps:
(1) selecting 50 volunteers with different blood lipid contents, extracting blood, and testing and recording the blood lipid content of each volunteer by using a blood lipid biochemical analyzer;
(2) measuring the near-infrared transmission spectrum blood fat characteristic peak of each volunteer, wherein the measuring process comprises the following steps:
(2-1) under the condition that the external environment condition is stable and appropriate, collecting the heart rate of the human body in real time by using a heart rate measuring instrument 20 and inputting a heart rate signal into a reference signal input end of a phase-locked amplifier 18; selecting a human body detection part, respectively fixing a first optical fiber probe 10 and a second optical fiber probe 11 on the same detection part of the human body (in the embodiment, the middle part of a thumb nail of a human hand), and respectively contacting the first optical fiber probe 10 and the second optical fiber probe 11 with the same detection part of the human body;
(2-2) triggering the broadband light source laser 1, enabling laser to enter the optical fiber beam splitter 3 through the first optical fiber 2 for splitting to obtain two beams, namely a first beam and a second beam, taking the first beam as reference light and the second beam as signal light, enabling the first beam to enter the first focusing lens 6 through the second optical fiber 4, enabling the first beam to enter the first optical fiber probe 10 through the third optical fiber 8, irradiating the first optical fiber probe 11 to a human body detection part, obtaining a near infrared spectrum signal after blood scattering, enabling the near infrared spectrum signal to enter the third focusing lens 14 through the sixth optical fiber 12, and enabling the near infrared spectrum signal to enter a signal input end of the first near infrared spectrometer 16; after entering a second focusing lens 7 through a fourth optical fiber 5, a light beam enters a second optical fiber probe 11 through a fifth optical fiber 9, irradiates a human body detection part through the second optical fiber probe 11, is scattered by blood to obtain a near infrared spectrum signal, and enters a signal input end of a second near infrared spectrometer 17 after entering a fourth focusing lens 15 through a seventh optical fiber 13;
(2-3) directly inputting the output signal of the first near-infrared spectrometer 16 into the signal input end of the computer 19, and processing the output signal by the computer 19 to obtain a reference spectrum L10; the output signal of the second near-infrared spectrometer 17 enters the detection signal input end of the lock-in amplifier 18, meanwhile, the heart rate signal of the human body is input to the lock-in amplifier 18 as the reference signal through the reference signal input end, the lock-in amplifier 18 amplifies the near-infrared light signal in the blood, locks the spectral information in the blood, then inputs the spectral information to the signal input end of the computer 19 in a signal form, and the signal spectrum L20 is obtained by the processing of the computer 19;
(2-4) normalizing the reference spectrum L10 by the computer 19 using the characteristic peak of hemoglobin at 2298nm to obtain a spectrum L1; for the signal spectrum L20, the computer 19 performs normalization processing by using a hemoglobin characteristic peak at 2298nm to obtain a spectrum L2; subtracting the spectrum L1 from the spectrum L2, and dividing the spectrum L1 by the spectrum L0 of the broadband light source emitted by the output end of the broadband light source laser to obtain a near-infrared transmission spectrum T in the pure blood, namely T (L2-L1)/L0;
(2-5) carrying out normalization processing on the hemoglobin characteristic peak at 2298nm by using the blood lipid characteristic peak at 2222nm in the near-infrared transmission spectrum T by using the computer 19 to obtain the relative intensity of the blood lipid characteristic peak of the human body near-infrared transmission spectrum;
(3) according to the blood lipid characteristic peak relative intensity and the blood lipid content of the near infrared transmission spectrum of different volunteers, a least square method is utilized to fit and establish a relation curve of the blood lipid characteristic peak relative intensity and the blood lipid content of the near infrared transmission spectrum, as shown in fig. 4;
(4) and (3) for the person to be detected needing noninvasive blood lipid detection, carrying out measurement in the steps (2-1) to (2-5) once to obtain the actually measured near-infrared transmission spectrum blood lipid characteristic peak relative intensity of the person to be detected, and substituting the actually measured near-infrared transmission spectrum blood lipid characteristic peak relative intensity into the relation curve established in the step (3) to obtain the real-time blood lipid content of the person to be detected.
Claims (10)
1. A noninvasive blood lipid detection device based on near infrared spectrum is characterized by comprising a broadband light source laser, an optical fiber beam splitter, a first focusing lens, a second focusing lens, a first optical fiber probe, a second optical fiber probe, a third focusing lens, a fourth focusing lens, a first near infrared spectrometer, a second near infrared spectrometer, a lock-in amplifier, a heart rate measuring instrument and a computer, wherein the optical fiber beam splitter comprises an input end and two output ends, the output end of the broadband light source laser is connected with the input end of the optical fiber beam splitter through a first optical fiber, one output end of the optical fiber beam splitter is connected with the input end of the first focusing lens through a second optical fiber, the output end of the first focusing lens is connected with the input end of the first optical fiber probe through a third optical fiber, and the other output end of the optical fiber beam splitter is connected with the input end of the second focusing lens through a fourth optical fiber, the output end of the second focusing lens is connected with the input end of the second optical fiber probe through a fifth optical fiber, the first optical fiber probe and the second optical fiber probe are respectively used for collecting near infrared spectrum signals of a human body detection part, the output end of the first optical fiber probe is connected with the input end of the third focusing lens through a sixth optical fiber, the output end of the third focusing lens is connected with the signal input end of the first near infrared spectrometer, the signal output end of the first infrared spectrometer is connected with the signal input end of the computer, the output end of the second optical fiber probe is connected with the input end of the fourth focusing lens through a seventh optical fiber, the output end of the fourth focusing lens is connected with the signal input end of the second near infrared spectrometer, and the signal output end of the second infrared spectrometer is connected with the detection signal input end of the lock-in amplifier, the reference signal input end of the phase-locked amplifier is connected with the signal output end of the heart rate measuring instrument, the heart rate measuring instrument is used for measuring the heart rate of a human body, the amplification signal output end of the phase-locked amplifier is connected with the signal input end of the computer, the trigger signal output end of the computer is connected with the signal input end of the broadband light source laser, and the first near-infrared spectrometer and the second near-infrared spectrometer are respectively used for collecting near-infrared spectrum signals and splitting light to obtain spectrum data and convert the light signals into electric signals.
2. The noninvasive blood lipid detection device based on near infrared spectrum of claim 1, it is characterized in that the first optical fiber probe and the second optical fiber probe have the same structure and both comprise a hollow circular truncated cone body and a fifth focusing lens, the hollow cone is a lightproof hollow cone, the fifth focusing lens is embedded in the central hole of the hollow cone, the distance between the front end face of the fifth focusing lens and the front end face of the hollow cone is 2-5 mm, a connecting optical fiber is arranged at the rear end of the central hole of the hollow cone body in a penetrating way, the connecting optical fiber is over against the rear end of the fifth focusing lens, and the connecting optical fibers in the first optical fiber probe and the second optical fiber probe are respectively connected with the third optical fiber and the fifth optical fiber.
3. The noninvasive blood lipid detection device based on near infrared spectroscopy of claim 2, wherein the first focusing lens, the second focusing lens, the third focusing lens, the fourth focusing lens and the fifth focusing lens are all broadband achromatic lenses, the numerical aperture of each achromatic lens is 0.25, and the focal length of each achromatic lens is 4 mm.
4. The noninvasive blood lipid detection device based on near infrared spectrum of claim 2, wherein the first optical fiber, the second optical fiber, the third optical fiber, the fourth optical fiber, the fifth optical fiber, the sixth optical fiber, the seventh optical fiber and the connecting optical fiber are quartz single mode fibers.
5. The noninvasive blood lipid detection device based on near infrared spectroscopy as claimed in claim 1, wherein the broadband light source emitted by the output end of the broadband light source laser is supercontinuum laser, the wavelength range is 600 nm-2500 nm, and the power is 500 mv.
6. The noninvasive blood lipid detection device based on near infrared spectroscopy as claimed in claim 1, wherein the optical fiber used by the optical fiber beam splitter is a quartz optical fiber, the transmittance is greater than 98%, the splitting ratio is 50:50, and the deviation of the splitting ratio is ± 4%.
7. The noninvasive blood lipid detection device based on near infrared spectrum of claim 1, wherein the first near infrared spectrometer and the second near infrared spectrometer have a measurement range of 500-2500 nm and a resolution better than 0.5 nm.
8. The noninvasive blood lipid detection device based on near infrared spectroscopy as claimed in claim 1, wherein the measurement range of the heart rate measurement instrument is 40-200 pulse/min, and the measurement error is ± 5%.
9. The noninvasive blood lipid detection device based on near infrared spectroscopy of claim 1, wherein the human body detection site is the middle of the thumb nail of a human hand.
10. A method of non-invasive blood lipid testing using the device of any one of claims 1-9, comprising the steps of:
(1) selecting a plurality of volunteers with different blood lipid contents, extracting blood of the volunteers, and testing and recording the blood lipid content of each volunteer by using a blood lipid biochemical analyzer;
(2) measuring the near-infrared transmission spectrum blood fat characteristic peak of each volunteer, wherein the measuring process comprises the following steps:
(2-1) under the condition that the external environment condition is stable and appropriate, collecting the heart rate of the human body in real time by using a heart rate measuring instrument and inputting a heart rate signal into a reference signal input end of a phase-locked amplifier; selecting a human body detection part, respectively fixing a first optical fiber probe and a second optical fiber probe on the same human body detection part, and respectively contacting the first optical fiber probe and the second optical fiber probe with the same human body detection part;
(2-2) triggering a broadband light source laser, enabling laser to enter an optical fiber beam splitter through a first optical fiber for splitting to obtain two beams, namely a first beam and a second beam, taking the first beam as reference light and the second beam as signal light, enabling the first beam to enter a first focusing lens through a second optical fiber, then enabling the first beam to enter a first optical fiber probe through a third optical fiber, irradiating the first optical fiber probe to a human body detection part, obtaining a near infrared spectrum signal after blood scattering, enabling the near infrared spectrum signal to enter a signal input end of a first near infrared spectrometer after entering a third focusing lens through a sixth optical fiber; after entering a second focusing lens through a fourth optical fiber, a light beam enters a second optical fiber probe through a fifth optical fiber, irradiates a human body detection part through the second optical fiber probe, is scattered by blood to obtain a near infrared spectrum signal, and enters a signal input end of a second near infrared spectrometer after entering a fourth focusing lens through a seventh optical fiber;
(2-3) directly inputting an output signal of the first near-infrared spectrometer into a signal input end of a computer, and processing by the computer to obtain a reference spectrum L10; the output signal of the second near-infrared spectrometer enters the detection signal input end of the lock-in amplifier, meanwhile, the heart rate signal of the human body is used as a reference signal and is input to the lock-in amplifier through the reference signal input end, the lock-in amplifier amplifies the near-infrared light signal in the blood, the spectrum information in the blood is locked, then the spectrum information is input to the signal input end of the computer in a signal form, and the signal spectrum L20 is obtained through the processing of the computer;
(2-4) normalizing the reference spectrum L10 by using a computer with a hemoglobin characteristic peak at 2298nm to obtain a spectrum L1; for the signal spectrum L20, normalizing by a computer by using a hemoglobin characteristic peak at 2298nm to obtain a spectrum L2; subtracting the spectrum L1 from the spectrum L2, and dividing the spectrum L1 by the spectrum L0 of the broadband light source emitted by the output end of the broadband light source laser to obtain a near-infrared transmission spectrum T in the pure blood, namely T (L2-L1)/L0;
(2-5) carrying out normalization processing on the hemoglobin characteristic peak at 2298nm by using the blood lipid characteristic peak at 2222nm in the near-infrared transmission spectrum T by using a computer to obtain the relative intensity of the blood lipid characteristic peak of the human body near-infrared transmission spectrum;
(3) according to the relative intensities of the blood lipid characteristic peaks and the blood lipid contents of the near-infrared transmission spectra of different volunteers, fitting and establishing a relation curve of the relative intensities of the blood lipid characteristic peaks and the blood lipid contents of the near-infrared transmission spectra by using a least square method;
(4) and (3) for the person to be detected needing noninvasive blood lipid detection, carrying out measurement in the steps (2-1) to (2-5) once to obtain the actually measured near-infrared transmission spectrum blood lipid characteristic peak relative intensity of the person to be detected, and substituting the actually measured near-infrared transmission spectrum blood lipid characteristic peak relative intensity into the relation curve established in the step (3) to obtain the real-time blood lipid content of the person to be detected.
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| CN104367327A (en) * | 2014-11-12 | 2015-02-25 | 杭州欣安医疗科技有限公司 | Detecting device and method for detecting human body biochemical parameters based on near-infrared rays |
| US20150369725A1 (en) * | 2013-01-31 | 2015-12-24 | Universidade Do Minho | Optical system for parameter characterization of an element of body fluid or tissue |
| US20200367756A1 (en) * | 2018-01-12 | 2020-11-26 | Agency For Science, Technology And Research | Method and system for in vivo detection of adipose tissue browning |
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| US20150369725A1 (en) * | 2013-01-31 | 2015-12-24 | Universidade Do Minho | Optical system for parameter characterization of an element of body fluid or tissue |
| CN103349553A (en) * | 2013-08-01 | 2013-10-16 | 赵巍 | Double-wavelength differential near-infrared non-invasive glucose meter |
| CN104367327A (en) * | 2014-11-12 | 2015-02-25 | 杭州欣安医疗科技有限公司 | Detecting device and method for detecting human body biochemical parameters based on near-infrared rays |
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