CN111323517B - Content determination method - Google Patents
Content determination method Download PDFInfo
- Publication number
- CN111323517B CN111323517B CN202010279949.5A CN202010279949A CN111323517B CN 111323517 B CN111323517 B CN 111323517B CN 202010279949 A CN202010279949 A CN 202010279949A CN 111323517 B CN111323517 B CN 111323517B
- Authority
- CN
- China
- Prior art keywords
- calycosin glucoside
- aqueous solution
- calycosin
- performance liquid
- high performance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/30—Control of physical parameters of the fluid carrier of temperature
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
Abstract
The invention discloses a content determination method, which relates to the technical field of medicine detection and comprises the following steps: s1, taking a decoction paste water solution, wherein the water solution contains calycosin glucoside; s2, extracting the aqueous solution by an online solid-phase extraction method to obtain a calycosin glucoside aqueous solution; and S3, measuring the calycosin glucoside aqueous solution by using a high performance liquid chromatography to determine the content of the calycosin glucoside. When the calycosin glucoside in the decocted extract is measured, the combination of the on-line solid phase extraction and the high performance liquid chromatography can overcome the defect that the calycosin glucoside is difficult to separate due to high viscosity of the decocted extract, and the method has the advantages of good repeatability, high sensitivity and short analysis time, can be used as a method for measuring the content of the calycosin glucoside in liver and kidney nourishing, and further lays a certain foundation for improving the quality standard of the decocted extract, ensuring the quality of the medicine and the like.
Description
Technical Field
The invention relates to the technical field of medicine detection, in particular to a content determination method.
Background
At present, liver and kidney nourishing is a soft extract prepared from wolfberry fruit, astragalus root, codonopsis pilosula, ophiopogon root and donkey-hide gelatin, and is collected in the Chinese medicinal preparation (second book) of drug standards of Ministry of health of the people's republic of China. Has effects in nourishing liver, improving eyesight, nourishing yin, and invigorating kidney, and can be used for treating kidney yin deficiency, deficiency of both qi and blood, blurred vision, vexation, insomnia, listlessness, debilitation, soreness of waist and legs, etc. According to the compatibility characteristics of the prescription, the contents of betaine in the medlar and calycosin glucoside in the astragalus root are used as the liver and kidney quality control standards. The determination of calycosin glucoside in astragalus root is carried out by high performance liquid chromatography stipulated in 'Chinese pharmacopoeia' of 2015 edition, but after liver and kidney nourishing medicinal materials are decocted by water, dregs are removed and concentrated, a semi-fluid preparation prepared by adding refined honey is high in viscosity, the determination sensitivity is lower by using a traditional high performance liquid chromatography, and the method is very necessary for further perfecting the quality standard of the decocted ointment, ensuring the medicine quality and establishing the determination method of the content of calycosin glucoside in the decocted ointment.
Disclosure of Invention
The invention aims at the problems and provides a content measuring method which comprises the following steps:
s1, taking a decoction paste water solution, wherein the water solution contains calycosin glucoside;
s2, extracting the aqueous solution by an online solid-phase extraction method to obtain a calycosin glucoside aqueous solution;
and S3, measuring the calycosin glucoside aqueous solution by using a high performance liquid chromatography to determine the content of the calycosin glucoside.
In some embodiments, in S1, the aqueous decoction extract solution includes:
taking the soft extract, and placing in a volumetric flask;
adding water to scale, shaking, centrifuging, and collecting supernatant to obtain the aqueous solution of the soft extract.
In some embodiments, the concentration of the aqueous decoct solution is 200g/L.
In some embodiments, in S2 and S3, both the extraction column of the online solid phase extraction method and the analysis column of the high performance liquid chromatography method use octadecylsilane chemically bonded silica as a filler.
In some embodiments, the assay method further comprises:
taking a plurality of calycosin glucosides with reference concentrations, and testing the peak areas of the calycosin glucosides with the reference concentrations by adopting a high performance liquid chromatography;
determining a linear relation according to the control concentration and the peak area;
correspondingly, the determination of the calycosin glucoside aqueous solution by the high performance liquid chromatography method to determine the content of the calycosin glucoside comprises the following steps:
taking the water solution of the decocted extract, extracting the water solution by on-line solid phase extraction, testing by adopting a high performance liquid chromatography to obtain the peak area of calycosin glucoside in the decocted extract, and determining the content of the calycosin glucoside in the water solution of the decocted extract according to the linear relation.
In some embodiments, the plurality of control concentrations of calycosin glucoside has a concentration of 0.1101mg/ml, 0.2202mg/ml, 0.4404mg/ml, 0.8808mg/ml, 1.7616mg/ml, respectively.
In some embodiments, the linear relationship is:
y =0.6147X-0.005 r =0.9999, wherein X is the concentration of calycosin glucoside, Y is the peak area, and r is the linear correlation coefficient.
In some embodiments, the high performance liquid chromatography uses acetonitrile as mobile phase a and water with 0.2% formic acid as mobile phase B.
In some embodiments, the elution conditions for the high performance liquid chromatography gradient elution are:
the invention has the advantages that: when the calycosin glucoside in the decocted extract is measured, the solid-phase extraction and the high performance liquid chromatography are combined, so that the defect that the calycosin glucoside is difficult to separate due to high viscosity of the decocted extract can be overcome, the method is good in repeatability, high in sensitivity and short in analysis time, can be used as a method for measuring the content of the calycosin glucoside in liver and kidney nourishing, and further lays a certain foundation for improving the quality standard of the decocted extract, ensuring the quality of a medicine and the like.
In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below with reference to the drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention.
FIG. 1 is a flow chart of a method for measuring a content of example 1 of the present invention;
FIG. 2 is a schematic diagram of the flow path of an on-line solid-phase extraction and high-efficiency system in a content measurement method according to an embodiment of the present invention;
FIG. 3 is a high performance liquid chromatogram of calycosin glucoside measured by a content measurement method according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
FIG. 1 is a flowchart of a method for measuring the content of calycosin glucoside in a soft extract of example 1 of the present invention; as shown in fig. 1, a content measuring method includes the following steps:
s1, taking a decoction paste water solution, wherein the water solution contains calycosin glucoside;
s2, extracting the aqueous solution by an online solid-phase extraction method to obtain a calycosin glucoside aqueous solution;
and S3, measuring the calycosin glucoside aqueous solution by using a high performance liquid chromatography to determine the content of the calycosin glucoside.
In the embodiment of the application, online solid-phase extraction and high performance liquid chromatography are adopted, wherein an analytical column and an extraction column both use octadecylsilane chemically bonded silica as fillers; performing gradient elution by high performance liquid chromatography with acetonitrile as mobile phase A and 0.2% formic acid solution as mobile phase B; wherein the column temperature is 20-40 ℃; the detection wavelength is 262nm; the number of theoretical plates is not less than 110000 calculated according to the calycosin glucoside peak.
When the calycosin glucoside in the decocted extract is measured, the solid-phase extraction and the high performance liquid chromatography are combined, so that the defect that the calycosin glucoside is difficult to separate due to high viscosity of the decocted extract can be overcome, the method is good in repeatability, high in sensitivity and short in analysis time, can be used as a method for measuring the content of the calycosin glucoside in liver and kidney nourishing, and further lays a certain foundation for improving the quality standard of the decocted extract, ensuring the quality of a medicine and the like.
In some embodiments, in S1, the aqueous decoction extract solution includes:
taking the soft extract, and placing in a volumetric flask;
adding water to scale, shaking, centrifuging, and collecting supernatant to obtain the aqueous solution of the soft extract.
In some embodiments, the concentration of the aqueous soft extract solution is 200g/L.
In the embodiment of the application, the soft extract is prepared into a proper concentration through a plurality of tests, so that the separation and extraction of calycosin glucoside are easy, the soft extract can be used as a method for measuring the content of calycosin glucoside in liver and kidney nourishing, and further, a certain foundation is laid for improving the quality standard of the soft extract, ensuring the medicine quality of the soft extract.
In some embodiments, in S2 and S3, both the extraction column of the online solid-phase extraction method and the analytical column of the high performance liquid chromatography adopt octadecylsilane chemically bonded silica as a filler.
In some embodiments, the assay method further comprises:
taking a plurality of calycosin glucosides with reference concentrations, and testing the peak areas of the calycosin glucosides with the reference concentrations by adopting a high performance liquid chromatography;
determining a linear relation according to the control concentration and the peak area;
correspondingly, the determination of the calycosin glucoside aqueous solution by high performance liquid chromatography to determine the content of the calycosin glucoside comprises the following steps:
taking the aqueous solution of the decocted extract, extracting the aqueous solution by online solid-phase extraction, testing by adopting a high performance liquid chromatography to obtain the peak area of verbascone isoflavone glucoside in the decocted extract, and determining the content of the verbascone isoflavone glucoside in the aqueous solution of the decocted extract according to the linear relation.
In some embodiments, the plurality of control concentrations of calycosin glucoside has a concentration of 0.1101mg/ml, 0.2202mg/ml, 0.4404mg/ml, 0.8808mg/ml, 1.7616mg/ml, respectively.
In some embodiments, the linear relationship is:
y =0.6147X-0.005 r =0.9999, wherein X is the concentration of calycosin glucoside, Y is the peak area, and r is the linear correlation coefficient.
In some embodiments, the high performance liquid chromatography uses acetonitrile as mobile phase a and water with 0.2% formic acid as mobile phase B.
In some embodiments, the elution conditions for the high performance liquid chromatography gradient elution are:
in some embodiments, the gradient elutes in a sample size of 15 to 25 μ L.
In some embodiments, the flow rates of the mobile phases are each 1.0mL/min.
In some embodiments, the mass concentration of the water solution of the soft extract is 150-250 g/L.
In some embodiments, the mass concentration of the aqueous decoction extract solution is 200g/L.
In some embodiments, the composition further comprises a 1mg/L solution of calycosin glucoside as a control.
In some embodiments, the column temperature is 30 ℃.
Examples of the experiments
1. Instrument and reagent
1.1 instruments
A Saimer Feishale Ultimate3000 dual-ternary high performance liquid chromatograph; an Shimadzu AUW200D electronic balance; sartorius BSA224S electronic balance; shim-pack VP-ODS analytical column Shimadzu (150 x 4.6mm 5um); semer femalim Acclaim HPLC WCX extraction column (10 × 4.6mm 5um).
1.2 reagents
Calycosin glucoside reference (China institute for testing food and drug, lot No. 111920-201203); liver and kidney nourishing essence (inner Mongolia Datang pharmaceutical Co., ltd. # 16833001, 16833002, 16833004, 17833001, 17833002, 17833005, 17833021, 18833001); acetonitrile is chromatographically pure; the others are analytically pure; the water is ion exchange high purity water.
(1) Preparation of control solutions: taking a proper amount of calycosin glucoside reference substance, precisely weighing, and adding water to obtain 1ug of solution containing calycosin glucoside per 1 ml.
(2) Preparation of a test solution: taking about 1g of liver and kidney nourishing, precisely weighing, placing in a 5ml volumetric flask, adding a proper amount of water, shaking up, adding water to scale, shaking up, centrifuging, and taking supernatant to obtain the product.
(3) Preparing a negative control solution: preparing a negative sample without astragalus root according to a liver-kidney nourishing prescription process, and preparing a negative control solution according to the preparation method of the test solution.
2.1 chromatographic conditions and system applicability test analysis columns and extraction columns all use octadecylsilane chemically bonded silica as filling agents; on-line solid phase extraction analysis was performed using acetonitrile as mobile phase a and water (containing 0.2% formic acid) as mobile phase B according to the flow path and the method of table 1 below; the column temperature is 30 ℃; the detection wavelength is 262nm; the number of theoretical plates is not less than 110000 calculated according to the calycosin glucoside peak.
The chromatograph flow path is connected:
sample pump (left pump) → sample injector → six-way valve interface 1
Analytical pump (right pump) → six-way valve interface 3
Six-way valve interface 2 → solid phase extraction column → six-way valve interface 5
Six-way valve interface 4 → analytical column → UV detector → waste liquid
Six-way valve interface 6 → waste
The experiment adopts a valve switching technology, the used switching valve is a two-position six-way valve, fig. 2 is a schematic flow path diagram of an on-line solid phase extraction-high performance liquid chromatography system of calycosin glucoside in the decocted extract, and referring to fig. 2, the system flow path connection is combined with a chromatograph flow path connection to carry out the experiment.
In the experimental process, the switching valve is in a 1-2 connection state after 0-3.0 min, the solid-phase extraction pump sucks a sample to the solid-phase extraction column, and calycosin glucoside in the sample is retained in the solid-phase extraction column, while other substances are not retained and directly flow into waste liquid; 3.0-8.0 min, the switching valve is in 6-1 connection state, and the mobile phase of the analysis pump transfers calycosin glucoside from the solid-phase extraction column to the analysis column; 8.0-17.01 min, the switching valve is in 1-2 connection state, the solid phase extraction pump washes the solid phase extraction column and balances for the next needle sample, and the analysis pump continues to complete the separation of calycosin glucoside. The specific elution procedure is shown in table 1.
TABLE 1 gradient elution conditions
* The left pump mobile phase takes the retention time of a reference substance in a solid phase extraction column as reference, and the time from the peak starting point to the falling point is between 11 and 12.8 min.
2. Specialization inspection
According to the determined chromatographic conditions, 20ul of each of the reference solution, the test solution and the negative reference solution is respectively absorbed and injected into a chromatograph for analysis.
FIG. 3 is a high performance liquid chromatogram of calycosin glucoside in a soft extract according to an embodiment of the present invention, as shown in FIG. 3, and a negative control chromatogram is shown in FIG. 3; the middle is a chromatogram of a test sample; the lower control is the chromatogram.
As can be seen from FIG. 3, the chromatogram of the test sample has a chromatographic peak at the same position as the retention time of the chromatogram of the control sample, and the separation effect is good, while the chromatogram of the negative control sample has no chromatographic peak at the same position as the retention time of the chromatogram of the control sample, indicating that the method is negative, free of interference and good in specificity.
3. Linear relation
Taking 11.01mg (content: 97.3%) of calycosin glucoside reference substance, putting into a 100ml measuring flask, adding water to dissolve, diluting to scale, shaking, precisely sucking 1ml to a 50ml volumetric flask, adding water to dilute to scale, and shaking (containing calycosin glucoside 2.202 ug/ml). Precisely sucking 1ul, 2ul, 4ul, 8ul, 16ul and 24ul, injecting into chromatograph, measuring according to the above chromatographic conditions (Table 1), and performing regression analysis (Table 2) on the corresponding concentration by peak area for analysis.
TABLE 1 gradient elution conditions
TABLE 2 measurement of standard curve
The results show that: the calycosin glucoside has good linear relation in the range of 0.1101 mg/ml-2.6424 mg/ml. The regression equation is:
Y=0.6147X-0.005 r=0.9999
4. precision degree
The liver and kidney nourishing medicine (batch No. 17833001) is taken to prepare the test solution, the sample is continuously injected for 6 times, the sample injection amount is 20ul, the peak area of calycosin glucoside is measured, and the result is shown in Table 3.RSD (n = 6) was 0.015%, indicating good precision of the instrument.
TABLE 3 results of the precision test
5. Repeatability test
The same batch (17833001) of Ganqi 6 parts (about 1.0g per sample) was sampled and measured according to the preparation of test solution and chromatographic conditions, and the sample size was 20ul, and the results are shown in Table 4. The average content of calycosin glucoside in liver and kidney nourishing is 5.04ug/g, and RSD is 4.72%, which shows that the method has good repeatability.
TABLE 4 results of repeated experiments
6. Stability test
The liver and kidney nourishing substances (batch No. 17833001) were measured according to the preparation of the test solution and the chromatographic conditions, respectively, the sample size was 20ul, and the results are shown in Table 5 after 0, 0.5, 2, 4, 8 and 17 h. RSD is 4.16%, which indicates that the test solution is stable within 17 h.
TABLE 5 stability test results
7. Sample application recovery rate test
Taking the same batch number (17833001, wherein the average content of calycosin glucoside in liver and kidney essence is 5.04 ug/g), precisely weighing 6 parts (about 0.5g per sampling amount) of liver and kidney essence, placing into a 5ml measuring flask, precisely adding 1.0, 2.0, and 3.0ml of reference solution (containing 1.101ug/ml of calycosin glucoside in reference solution), adding appropriate amount of water, shaking, adding water to scale, shaking, centrifuging, collecting supernatant, measuring according to the above chromatographic conditions, and measuring the sample volume to be 20ul, the results are shown in Table 6. Indicating that the process has good recovery.
TABLE 6 sample application recovery rate test results
8. Content determination of test sample
The liver and kidney nourishing substances are taken to prepare a test solution, the content of calycosin glucoside in 8 batches of liver and kidney nourishing substances is measured (n = 2), the result is shown in table 7, and the data of 8 batches of samples show that the content of calycosin glucoside in the liver and kidney nourishing substances is more than 0.97 ug/g.
TABLE 7 measurement results of samples
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A content measurement method is characterized by comprising the following steps:
s1, taking an aqueous solution of a liver and kidney nourishing decoction paste, wherein the aqueous solution contains calycosin glucoside;
s2, extracting the aqueous solution by an online solid-phase extraction method to obtain a calycosin glucoside aqueous solution;
s3, determining the content of calycosin glucoside by using high performance liquid chromatography to determine the calycosin glucoside aqueous solution;
in S2 and S3, an extraction column of an online solid-phase extraction method and an analysis column of a high performance liquid chromatography both adopt octadecylsilane chemically bonded silica as filling agents; acetonitrile is taken as a mobile phase A, water containing 0.2 percent formic acid is taken as a mobile phase B, and the elution conditions of gradient elution are as follows:
2. the method according to claim 1, wherein in S1, the step of collecting an aqueous solution of a soft extract comprises:
taking the soft extract, and placing in a volumetric flask;
adding water to the scale, shaking, centrifuging, and collecting supernatant to obtain the water solution of the soft extract.
3. The method according to claim 1, wherein the concentration of the aqueous solution of the soft extract is 200g/L.
4. The assay method according to claim 1, further comprising:
taking a plurality of calycosin glucoside with reference concentrations, and testing the peak areas of the calycosin glucoside with the reference concentrations by adopting a high performance liquid chromatography;
determining a linear relation according to the comparison concentration and the peak area;
correspondingly, the determination of the calycosin glucoside aqueous solution by high performance liquid chromatography to determine the content of the calycosin glucoside comprises the following steps:
taking the water solution of the soft extract, extracting the water solution by using online solid-phase extraction, testing by using high performance liquid chromatography to obtain the peak area of calycosin glucoside in the soft extract, and determining the content of the calycosin glucoside in the water solution of the soft extract according to the linear relation.
5. The assay method according to claim 4, wherein the concentration of calycosin glucosides at the plurality of control concentrations is 0.1101mg/ml, 0.2202mg/ml, 0.4404mg/ml, 0.8808mg/ml, 1.7616mg/ml, respectively.
6. The assay of claim 5, wherein the linear relationship is:
y =0.6147X-0.005 r =0.9999, wherein X is the concentration of calycosin glucoside, Y is the peak area, and r is the linear correlation coefficient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010279949.5A CN111323517B (en) | 2020-04-10 | 2020-04-10 | Content determination method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010279949.5A CN111323517B (en) | 2020-04-10 | 2020-04-10 | Content determination method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111323517A CN111323517A (en) | 2020-06-23 |
| CN111323517B true CN111323517B (en) | 2023-02-24 |
Family
ID=71164421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010279949.5A Active CN111323517B (en) | 2020-04-10 | 2020-04-10 | Content determination method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111323517B (en) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181857A (en) * | 2015-09-07 | 2015-12-23 | 国家烟草质量监督检验中心 | Method for measuring polycyclic aromatic hydrocarbons in cigarette cut tobacco by on-line solid phase extraction and high performance liquid chromatography |
| CN108226331B (en) * | 2017-12-26 | 2020-09-22 | 贵州益佰制药股份有限公司 | Detection method of anti-tumor injection preparation |
| CN109061004B (en) * | 2018-09-20 | 2021-06-25 | 广西壮族自治区药用植物园 | Method for measuring content of calycosin glucoside in antitoxic leukogenic mixture |
| CN110568108B (en) * | 2019-10-09 | 2022-03-01 | 湖南中医药大学 | Multi-component content determination method of Ganfule preparation |
-
2020
- 2020-04-10 CN CN202010279949.5A patent/CN111323517B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN111323517A (en) | 2020-06-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102072846B (en) | Method for detecting quality of compound capsule prepared from 8 kinds of amino acids and 11 kinds of vitamins | |
| CN111879887B (en) | Radix astragali medicinal material and detection method and application of components in preparation thereof | |
| CN103822975B (en) | A kind of detection method of formulation of ' Sheng Mai ' of Radix Codonopsis side | |
| CN102928520A (en) | Determination method for content of saccharide components in mongolian milkvetch root extract | |
| CN110568111B (en) | Method for detecting oligosaccharide in morinda officinalis formula particles | |
| CN111323517B (en) | Content determination method | |
| CN101474249B (en) | Motherwort and quality control method of preparation thereof | |
| CN110967422A (en) | Method for determining content of astragaloside in qi-tonifying and blood-nourishing tablet | |
| CN106404685B (en) | The measuring method of total saponin content in a kind of health liquor | |
| CN102335333A (en) | Method for detecting traditional Chinese medicine preparation of compound nutritions paste | |
| CN105675734A (en) | Method for detecting oligosaccharide component content in compound salvia miltiorrhiza bge extract | |
| CN111398505B (en) | Method for simultaneously detecting contents of five components of traditional Chinese medicine for treating infantile enuresis | |
| CN101112422A (en) | Quality control method of particles for eliminating phlegm and stopping cough for children | |
| CN107247106A (en) | It is a kind of at the same determine Zhikening sucapsule in three kinds of active components method | |
| CN110967415B (en) | Method for measuring verbascoside in serum-nourishing brain water extract | |
| CN113504325A (en) | Detection method of changyanning preparation | |
| CN113504326A (en) | Detection method of changyanning preparation | |
| CN107064329A (en) | The content assaying method of five kinds of Ginsenosides in a kind of Xueshuan xinmaining Tablet | |
| CN107807182B (en) | Method for measuring content of ganoderic acid A in ganoderma lucidum syrup | |
| CN110618220A (en) | Method for detecting content of aristolochic acid I in Shangtongning capsules | |
| CN112415115B (en) | Detection method of blood-replenishing and milk-producing preparation | |
| CN115266971B (en) | Method for measuring contents of various components in Yinqiao cold bagged steeping preparation and one-measurement-multiple-evaluation detection method of Yinqiao cold bagged steeping preparation | |
| CN104215708B (en) | For treating the detection method of content of the Chinese native medicine compound prescription pellet of gynecologic blood diseases | |
| CN116879463B (en) | Quantitative detection method and application of gymnema sylvestre saponin C in gymnema sylvestre She Yaowu | |
| CN103267812B (en) | Method for detecting quality of Bazhen particles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |