CN111317031B - Additive-free normal-temperature yoghourt and preparation method thereof - Google Patents

Additive-free normal-temperature yoghourt and preparation method thereof Download PDF

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CN111317031B
CN111317031B CN201811539195.1A CN201811539195A CN111317031B CN 111317031 B CN111317031 B CN 111317031B CN 201811539195 A CN201811539195 A CN 201811539195A CN 111317031 B CN111317031 B CN 111317031B
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fermented
temperature
milk
fermentation
nostoc
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CN111317031A (en
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荆培培
冯志宽
李洪亮
孙涛
李树森
张冬洁
康正雄
韩建军
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Inner Mongolia Mengniu Dairy Group Co Ltd
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Inner Mongolia Mengniu Dairy Group Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/125Casei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/137Delbrueckii
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/147Helveticus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/165Paracasei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/173Reuteri
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus

Abstract

The invention belongs to the technical field of preparation of dairy food, and particularly relates to normal-temperature yogurt without additives and a preparation method thereof. The main raw materials of the normal-temperature yoghourt only comprise three main components of liquid milk, a sweetening agent and nostoc sphaeroides, and no additional stabilizing agent or acidity regulator is needed to be added. After the product is placed for 5 months at normal temperature, the product basically has no bleeding, bad flavor and protein coagulation and precipitation phenomena, has stable mouthfeel and longer shelf life, and finally proves that the product has reasonable formula, good flavor and silky mouthfeel. According to the preparation method, the nostoc sphaeroides powder is subjected to hydration and separate fermentation treatment, so that not only can the dissolution of polysaccharide substances of the nostoc sphaeroides powder be effectively improved, but also the bad flavor brought by the nostoc sphaeroides can be modified, the viscosity and the stability of the product can be successfully improved in a green mode, and the clean room-temperature yoghourt is prepared.

Description

Additive-free normal-temperature yoghourt and preparation method thereof
Technical Field
The invention belongs to the technical field of preparation of dairy food, and particularly relates to normal-temperature yogurt without additives and a preparation method thereof.
Background
The normal temperature yoghourt is a milk product which is prepared by taking milk as a main raw material, sterilizing and fermenting to obtain a product rich in nutrient substances such as protein, calcium and the like, and performing pasteurization to inactivate lactic acid bacteria in the yoghourt. The normal temperature yoghourt product is popular with dairy food processing manufacturers and consumers due to the characteristics of unique taste and flavor, no need of refrigeration, convenience for logistics transportation and storage and the like.
The selection of the stabilizer in the normal-temperature yogurt is one of the key factors for determining the normal-temperature storage and the stability during the storage, and the normal-temperature yogurt in the prior art generally needs to be added with at least three or more stabilizers to achieve the stabilizing effect. However, with the consumer's greater understanding and demand for natural products, no artificial ingredients, no or little added foods are of great concern in China, especially in the dairy category.
In order to realize the additive-free dairy product, chinese patent document CN107821598A provides a preparation method of additive-free normal-temperature yoghourt, the main components of the additive-free normal-temperature yoghourt are non-resistant milk, sweetener and starch, the obtained product has smooth and refreshing mouthfeel, can be placed for 6 months at normal temperature, and has no phenomena of layered whey precipitation and the like. However, the product has strict requirements on the modification method of starch, the particle size and the content of amylopectin, otherwise, the stability of the yoghurt product is directly influenced. It is known that this technique uses physically modified starch as a stabilizer, while starch is a stabilizer that is conventional in the dairy field, and this technique is merely preferred for stabilizers and is not truly additive-free.
Therefore, if the normal-temperature yoghourt which is natural in raw material and does not contain the stabilizer can be provided, the fact that the normal-temperature yoghourt is not added is realized.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to overcome the defect that the normal-temperature yoghourt in the prior art contains a stabilizer, so that the natural normal-temperature yoghourt which is prepared from natural materials and does not contain a stabilizer and is really free of additives and the preparation method thereof are provided.
In order to solve the technical problems, the invention adopts the following technical scheme:
an additive-free normal temperature yoghourt comprises the following components in percentage by weight,
90-98% of fermented milk;
2 to 10 percent of fermented nostoc sphaeroids kutz.
Further, the fermentation end point acidity of the fermented milk is 90-100T degrees;
and/or the acidity of the fermentation end point of the fermented nostoc sphaeroides is 70-85T degrees.
Further, the fermented milk comprises the following raw materials:
910-960 parts of liquid milk;
80-100U/T of leaven;
and/or the fermented nostoc sphaeroids kutz comprises the following raw materials:
50-300 parts of gexian rice flour;
950-700 parts by weight of water;
the leaven is 50-200U/T.
Further, the fermented milk and/or the fermented nostoc sphaeroids kutz raw material also comprises 40-90 parts by weight of sweetener.
Further, the liquid milk is selected from at least one of whole milk, skimmed milk, whole goat milk, skimmed goat milk and camel milk; preferably, the protein content of the liquid milk is above 3.3%.
And/or the sweetener is at least one selected from white granulated sugar, crystalline fructose, fructose and erythritol;
and/or the leavening agent is selected from at least one of lactobacillus delbrueckii subspecies lactis, streptococcus thermophilus, lactobacillus acidophilus, lactobacillus helveticus, lactobacillus plantarum, lactobacillus reuteri, lactobacillus casei and lactobacillus paracasei.
The preparation method of the additive-free normal-temperature yoghourt comprises the following steps:
mixing fermented milk and fermented nostoc sphaeroids kutz at a certain proportion, sterilizing, cooling and filling to obtain the fermented milk.
Further, the preparation steps of the fermented milk are as follows: heating the liquid milk to 45-55 ℃, homogenizing, sterilizing, adding a leaven for fermentation, demulsifying and cooling when reaching the fermentation end point to obtain fermented milk;
the preparation method of the fermented nostoc sphaeroides comprises the following steps: heating water to 40-70 deg.C, adding radix Puerariae Amurensis powder, dissolving, hydrating, sterilizing, adding starter, fermenting, cooling to the end of fermentation, and making into fermented radix Puerariae Amurensis.
Further, the method also comprises the step of adding a sweetening agent in the preparation step of the fermented milk and/or the preparation step of the fermented nostoc sphaeroids kutz.
Further, the nostoc sphaeroids kutz powder is dissolved by high-speed shearing, wherein the high-speed shearing condition is that the rotating speed is 1000-2000 r/min, and the time is 10-20 min.
Further, the hydration treatment condition in the preparation step of the fermented nostoc sphaeroides is that the stirring rotating speed is 50-150 r/min, and the time is 10-30 min.
Further, the homogenizing pressure is 18-22MPa, and the temperature is 55-65 ℃.
Further, in the preparation step of the fermented milk, the sterilization temperature is 95-105 ℃, and the time is 3-8min;
and/or, in the preparation of the fermented nostoc sphaeroids kutz, the sterilization temperature is 90-110 ℃, and the time is 3-10min;
and/or in the mixed filling, the sterilization is pasteurization, preferably, the sterilization temperature is 75-85 ℃ and the time is 15-20s.
Further, in the preparation step of the fermented milk, the fermentation temperature is 37-43 ℃, and the fermentation end point is reached when the fermentation acidity is 70-85 DEG T;
and/or in the step of preparing the fermented nostoc sphaeroids kutz, the fermentation temperature is 30-45 ℃, and the fermentation end point is reached when the fermentation acidity is 90-100 DEG T.
Further, the grain diameter of the nostoc sphaeroids kutz rice flour is 20-100 mu m. The nostoc sphaeroids kutz rice flour is preferably prepared from high-quality nostoc sphaeroids kutz through the processes of drying, impurity removal, screening, crushing and the like, and the particle size range is 20-100 mu m. The yogurt prepared from the nostoc sphaeroids kutz powder has fine and smooth mouthfeel and good stability.
As a preferred embodiment, the preparation method of the no-additive normal-temperature yogurt comprises the following steps:
preparation of fermented milk:
(1) Heating liquid milk with the protein content of more than 3.3 percent to 45-55 ℃, adding a sweetening agent, and dissolving by a high-speed shearing machine, wherein the rotating speed of the high-speed shearing machine is 500-1000 r/min, and the dissolving time is 10-20 min;
(2) Heating the feed liquid to 55-65 ℃, and homogenizing by a homogenizer with the homogenizing pressure of 18-22 Mpa;
(3) Sterilizing the homogenized feed liquid at 95-105 ℃ for 3-8 minutes;
(4) Reducing the temperature of the sterilized feed liquid to 37-43 ℃, adding a fermenting agent for fermentation, performing demulsification when the fermentation acidity is 70-85 DEG T, and cooling to 18-25 ℃; and (5) standby.
Preparing fermented nostoc:
(1) Mixing the nostoc sphaeroids kutz powder with water at the temperature of 40-70 ℃, and dissolving the mixture by a high-speed shearing machine at the rotating speed of 1000-2000 rpm for 10-20 minutes. Under the rotating speed, the polysaccharide in the gexian rice flour is dissolved out and fully absorbed and dissolved;
(2) And hydrating the dissolved feed liquid at the stirring speed of 50-150 rpm for 10-30 min.
(3) Sterilizing the hydrated feed liquid at 90-110 deg.c for 3-10 min. At the sterilization temperature, the nostoc powder mixed solution does not have the gel phenomenon;
(4) Reducing the temperature of the sterilized feed liquid to 30-45 ℃, adding a fermenting agent for fermentation, stopping fermentation when the fermentation acidity is 90-100 DEG T, and cooling to 18-25 ℃; the inventor finds that when the acidity reaches 90 DEG T, the bad flavor of the nostoc sphaeroides can be well covered.
Mixing and filling:
(1) Mixing the cooled fermented milk 850-950 with fermented nostoc sphaeroids kutz 150-50, stirring for 15-30 min, pasteurizing at 75-85 deg.C for 15-20s, and storing under sterile condition;
(2) And (4) carrying out sterile filling on the sterilized feed liquid, wherein the preferable filling temperature is 15-20 ℃.
The technical scheme of the invention has the following advantages:
1. the normal-temperature yogurt without the additives provided by the invention is added with the fermented nostoc sphaeroides for the first time, so that the stabilizing effect of the polysaccharide component in the nostoc sphaeroides is fully utilized, and most importantly, the nostoc sphaeroides is added to just solve the problem of the common addition of the normal-temperature yogurt in the prior art. The technology utilizes fermented nostoc sphaeroids kutz to replace a conventional stabilizer, and the prepared product is stable at normal temperature and perfect in taste.
The normal-temperature yogurt without the additives provided by the invention has the advantages that the main raw materials only comprise three components of liquid milk, a sweetening agent and nostoc sphaeroides, and no stabilizer, acidity regulator and the like are additionally added, and the normal-temperature yogurt which is stable at normal temperature, perfect in taste, thick and pure in taste, mellow, silky and rich in nutrition and does not have the bad flavor of nostoc sphaeroides can be prepared by only using the three main raw materials through adding the nostoc sphaeroides powder.
After the yoghourt product provided by the invention is placed for 5 months at normal temperature, little whey is separated out, no protein coagulation phenomenon exists, the mouthfeel is smooth, the fermentation flavor is rich, the bad flavor of nostoc sphaeroides is avoided, the mouthfeel is stable, the long shelf life is realized, and no additive is really realized.
The nostoc sphaeroides selected by the normal-temperature yoghourt contains various amino acids, carbohydrates, polysaccharides, ascorbic acid, vitamin C, mineral elements such as phosphorus, sulfur, calcium, potassium and iron, trace elements such as zinc, copper and manganese and the like which are necessary for human bodies, wherein the calcium content is higher than that of common vegetables, and the nostoc sphaeroides is an excellent natural calcium-rich nutritional food. The nostoc sphaeroides is used as a member of new resource food approved by the state, has no toxic or side effect, and can be used as both medicine and food. Therefore, the normal-temperature yoghourt provided by the invention is rich in nutrition and also has a certain health-care effect.
The normal-temperature yoghourt provided by the invention can ensure that the stability of the product is better by further optimizing the dosage of each component.
2. The preparation method of the normal-temperature yogurt without the additives comprises the steps of firstly respectively preparing fermented milk and fermented nostoc sphaeroids kutz, then mixing and filling to obtain the yogurt product, dissolving nostoc sphaeroids kutz powder and water under high-speed shearing, then performing hydration, sterilizing, and finally adding a fermenting agent for fermentation treatment, wherein the particle size of the nostoc sphaeroids kutz powder can be thinned through high-speed shearing and hydration, cell walls are broken, and polysaccharide is dissolved out, so that the stability of the product system is ensured, and meanwhile, the nostoc sphaeroids kutz powder is subjected to separate fermentation treatment, so that the bad flavor (fishy smell) brought by the nostoc sphaeroids kutz is avoided.
According to the preparation method of the normal-temperature yogurt without the additives, provided by the invention, through selection of the dissolving condition, the specific condition during hydration and the sterilization condition, the nostoc powder mixed solution can be prevented from generating the gel phenomenon, and the hydration is not damaged, so that the polysaccharide in the nostoc powder mixed solution is dissolved out and fully dissolved by water absorption, the utilization rate of nostoc is improved, the product stability is enhanced, and meanwhile, the control of the fermentation end point can well cover the bad flavor of nostoc. In addition, the temperature of water is also a key control point during dissolution, the water is heated to 40-70 ℃, the nostoc sphaeroides powder is added, the water temperature is too low, the nostoc sphaeroides is not easy to dissolve, and polysaccharide is degraded if the water temperature is too high, so that the use is influenced.
According to the preparation method of the normal-temperature yogurt without the additive, the fat floating of the product in the shelf life can be effectively prevented and the stability of the product can be enhanced by selecting the homogenization condition and standardizing the fat balls in the milk-based fermented material.
The preparation method of the normal-temperature yogurt without the additive provided by the invention has the advantages that the pasteurization condition is selected, the inactivation of the leavening agent in the fermentation base material is ensured, and the product is prevented from becoming acid in the storage process under the normal-temperature condition.
According to the preparation method of the normal-temperature yogurt without the additives, the stability of the nostoc polysaccharide gel performance is ensured by selecting the filling temperature, and the stability of the product quality is ensured at the same time.
According to the preparation method of the normal-temperature yogurt without the additives, the grain size and the dosage of the gexian rice flour are optimized, so that the prepared normal-temperature yogurt is fine and smooth in taste and better in stability.
Detailed Description
The following examples are provided to further understand the present invention, not to limit the scope of the present invention, but to provide the best mode, not to limit the content and the protection scope of the present invention, and any product similar or similar to the present invention, which is obtained by combining the present invention with other prior art features, falls within the protection scope of the present invention.
The examples do not indicate specific experimental procedures or conditions, and can be performed according to the procedures or conditions of the conventional experimental procedures described in the literature in the field. The reagents or instruments used are conventional reagent products which are commercially available, and manufacturers are not indicated.
Example 1
The embodiment provides a normal-temperature yogurt without additives, which comprises the following specific components in part by weight:
fermented milk raw materials (in 1000 kg): milk: 910 kg, white granulated sugar: 90 kg, starter: 100U.
Fermented nostoc sphaeroids kutz raw material (1000 kg): nostoc sphaeroids kutz powder: 50kg, water: 950kg, starter: 200U.
Preparation of fermented milk:
(1) Heating high-quality fresh milk with the protein content of more than 3.3% to 55 ℃, adding white granulated sugar, and dissolving for 20 minutes at the rotating speed of 500 revolutions per minute by a high-speed shearing machine;
(2) Heating the feed liquid to 65 ℃, and homogenizing by a homogenizer at the homogenizing pressure of 18Mpa;
(3) Sterilizing the homogenized feed liquid at 105 deg.C for 3 min;
(4) Reducing the temperature of the sterilized feed liquid to 37 ℃, adding a streptococcus thermophilus starter for fermentation, performing emulsion breaking when the fermentation acidity is 85 DEG T, and cooling to 18 ℃; and (5) standby.
Preparing fermented nostoc:
(1) Mixing the nostoc sphaeroids kutz powder with water of 40 ℃, and dissolving the mixture through a high-speed shearing machine at the rotating speed of 1000 rpm for 10 minutes.
(2) And (3) hydrating the dissolved feed liquid for 10 minutes at a stirring speed of 50 revolutions per minute.
(3) Sterilizing the hydrated feed liquid at 90 deg.C for 10 min.
(4) Reducing the temperature of the sterilized feed liquid to 30 ℃, adding lactobacillus delbrueckii subspecies lactis starter for fermentation, stopping fermentation when the fermentation acidity is 90 DEG T, and cooling to 25 ℃;
mixing and filling:
(1) Mixing 850kg of cooled fermented milk with 150kg of fermented nostoc sphaeroids kutz, stirring for 15 minutes, pasteurizing at 85 ℃, sterilizing for 15 seconds, and aseptically storing;
(2) And (4) carrying out sterile filling on the sterilized feed liquid, wherein the filling temperature is 20 ℃.
The normal-temperature yogurt without additives prepared by the embodiment has the advantages of strong and pure and delicious yogurt taste, mellow and smooth flavor and rich nutrition. After the product is placed for 5 months at normal temperature, little whey is separated out, no protein coagulation phenomenon exists, the taste is smooth, the fermentation flavor is strong, and no bad flavor of nostoc sphaeroides exists.
Example 2
The embodiment provides a normal-temperature yogurt without additives, which comprises the following specific components in part by weight:
fermented milk raw materials (in 1000 kg): milk: 920 kg, white granulated sugar: 80 kg, starter: 90U.
Fermented nostoc sphaeroids kutz raw material (1000 kg): nostoc sphaeroids kutz powder: 100kg, water: 900kg, starter: 160U.
Preparation of fermented milk:
(1) Heating high-quality skimmed milk with protein content of more than 3.3% to 45 deg.C, adding white sugar, and dissolving with high-speed shearing machine at rotation speed of 1000 rpm for 10min;
(2) Heating the feed liquid to 55 deg.C, homogenizing with homogenizer at 22Mpa;
(3) Sterilizing the homogenized feed liquid at 95 ℃ for 8 minutes;
(4) Reducing the temperature of the sterilized feed liquid to 43 ℃, adding lactobacillus helveticus starter for fermentation, performing emulsion breaking when the fermentation acidity is 70 DEG T, and cooling to 25 ℃; and (5) standby.
Preparing fermented nostoc:
(1) Mixing the nostoc sphaeroids kutz powder with water of 45 ℃, and dissolving the mixture through a high-speed shearing machine at the rotating speed of 1500 rpm for 15 minutes.
(2) And (3) hydrating the dissolved feed liquid at 80 rpm for 20 minutes.
(3) Sterilizing the hydrated feed liquid at 95 deg.C for 8 min.
(4) Reducing the temperature of the sterilized feed liquid to 38 ℃, adding a lactobacillus plantarum ferment for fermentation, stopping fermentation when the fermentation acidity is 100 DEG T, and cooling to 18-25 ℃;
mix filling
(1) Mixing 900kg of cooled fermented milk with 100kg of fermented nostoc sphaeroides, stirring for 25 minutes, pasteurizing at 75 ℃, sterilizing for 20 seconds, and aseptically storing;
(2) Sterile filling the sterilized feed liquid, preferably at 18 ℃;
the normal-temperature yogurt without additives prepared by the embodiment has the advantages of strong and pure and delicious yogurt taste, mellow and smooth flavor and rich nutrition. After the product is placed for 5 months at normal temperature, the product basically has no whey precipitation, smooth texture, no fat floating and no bad flavor of nostoc sphaeroides.
Example 3
The embodiment provides a normal-temperature yogurt without additives, which comprises the following specific components in percentage by weight:
fermented milk raw materials (in 1000 kg): milk: 940 kg, erythrose: 60 kg, starter: 80U.
Fermented nostoc sphaeroids kutz raw material (1000 kg): nostoc sphaeroids kutz powder: 150kg, water: 850kg, starter: 120U, respectively.
Preparation of fermented milk:
(1) Heating high-quality fresh milk with the protein content of more than 3.3 percent to 50 ℃, adding erythrose, and dissolving for 15 minutes at the rotating speed of 800 revolutions per minute by a high-speed shearing machine;
(2) Heating the feed liquid to 60 deg.C, homogenizing with homogenizer at 20Mpa;
(3) Sterilizing the homogenized feed liquid at 100 deg.C for 5 min;
(4) Reducing the temperature of the sterilized feed liquid to 40 ℃, adding a lactobacillus plantarum ferment for fermentation, performing emulsion breaking when the fermentation acidity is 80 DEG T, and cooling to 20 ℃; and (5) standby.
Preparing fermented nostoc:
(1) Mixing the Gexian rice flour with 50 deg.C water, and dissolving at 1000 rpm for 20 min by high speed shearing machine.
(2) And (3) hydrating the dissolved feed liquid at 100 revolutions per minute for 15 minutes.
(3) Sterilizing the hydrated feed liquid at 100 deg.C for 6 min.
(4) Reducing the temperature of the sterilized feed liquid to 33 ℃, adding lactobacillus paracasei starter for fermentation, stopping fermentation when the fermentation acidity is 90 DEG T, and cooling to 20 ℃;
mix filling
(1) Mixing 950kg of cooled fermented milk with 50kg of fermented nostoc sphaeroids kutz, stirring for 20 minutes, pasteurizing at 80 ℃, sterilizing for 18 seconds, and aseptically storing;
(2) Sterile filling the sterilized feed liquid, preferably at 18 ℃;
the normal-temperature yogurt without additives prepared by the embodiment has the advantages of thick and pure yogurt taste, mellow and smooth mouthfeel and rich nutrition. The product is slightly separated out of whey after being placed for 5 months at normal temperature, has smooth texture and no bad flavor of nostoc sphaeroides.
Example 4
The embodiment provides a normal-temperature yogurt without additives, which comprises the following specific components in part by weight:
fermented milk raw materials (in 1000 kg): milk: 950kg, fructose: 50kg, starter: 90U.
Fermented nostoc sphaeroids kutz raw material (1000 kg): nostoc sphaeroids kutz powder: 200 kg, water: 800 kg, starter: 200U.
Preparation of fermented milk:
(1) Heating high-quality fresh goat milk with protein content of more than 3.3% to 52 ℃, adding fructose, and dissolving by a high-speed shearing machine at the rotating speed of 700 r/min for 15 min;
(2) Heating the feed liquid to 62 deg.C, homogenizing with homogenizer at 21Mpa;
(3) Sterilizing the homogenized feed liquid at 101 deg.C for 5 min;
(4) Reducing the temperature of the sterilized feed liquid to 41 ℃, adding lactobacillus casei starter for fermentation, performing emulsion breaking when the fermentation acidity is 82 DEG T, and cooling to 22 ℃; and (5) standby.
Preparing fermented nostoc:
(1) Mixing the Gexian rice flour with water of 60 deg.C, and dissolving at 2000 rpm for 10min by high speed shearing machine.
(2) And (3) hydrating the dissolved feed liquid at 150 rpm for 20 minutes.
(3) Sterilizing the hydrated material liquid at 105 deg.C for 5 min.
(4) Reducing the temperature of the sterilized feed liquid to 42 ℃, adding lactobacillus acidophilus starter for fermentation, stopping fermentation when the fermentation acidity is 95 ℃ T, and cooling to 23 ℃;
mix filling
(1) Mixing the cooled fermented milk 900 weight ratio with the fermented nostoc sphaeroids kutz 100 weight ratio, stirring for 28 minutes, pasteurizing at 82 ℃, sterilizing for 17 seconds, and aseptically storing;
(2) Sterile filling the sterilized feed liquid, preferably at 16 ℃;
the normal-temperature yogurt without additives prepared by the embodiment has the advantages of strong and pure and delicious yogurt taste, mellow and smooth flavor and rich nutrition. The product is slightly separated out of whey after being placed for 5 months at normal temperature, has smooth texture and no bad flavor of nostoc sphaeroides.
Example 5
The embodiment provides a normal-temperature yogurt without additives, which comprises the following specific components in part by weight:
fermented milk raw materials (in 1000 kg): milk: 960 kg, crystalline fructose: 40 kg, starter: 80U.
Fermented nostoc sphaeroids kutz raw material (1000 kg): nostoc sphaeroids kutz powder: 300 kg, water: 700 kg, starter: 200U.
Preparation of fermented milk:
(1) Heating high-quality fresh milk with protein content of more than 3.3% to 50 ℃, adding crystalline fructose, and dissolving by a high-speed shearing machine at the rotating speed of 900 rpm for 15 minutes;
(2) Heating the feed liquid to 60 deg.C, homogenizing with homogenizer at 20Mpa;
(3) Sterilizing the homogenized feed liquid at 100 deg.C for 6 min;
(4) Reducing the temperature of the sterilized feed liquid to 42 ℃, adding a fermentation lactobacillus starter for fermentation, performing emulsion breaking when the fermentation acidity is 83 DEG T, and cooling to 22 ℃; and (5) standby.
Preparing fermented nostoc:
(1) Mixing the Gexian rice flour with 70 deg.C water, and dissolving with high speed shearing machine at 2000 rpm for 20 min.
(2) And (3) hydrating the dissolved feed liquid for 80 r/min for 30 min.
(3) Sterilizing the hydrated material liquid at 110 deg.C for 3 min.
(4) Reducing the temperature of the sterilized feed liquid to 42 ℃, adding lactobacillus delbrueckii subspecies lactis starter for fermentation, stopping fermentation when the fermentation acidity is 100 DEG T, and cooling to 20 ℃;
mix filling
(1) Mixing the cooled 950 weight ratio of fermented milk with 50 weight ratio of fermented nostoc sphaeroids kutz, stirring for 16 minutes, pasteurizing at 85 ℃, sterilizing for 15 seconds, and aseptically storing;
(2) Carrying out aseptic filling on the sterilized feed liquid, wherein the preferable filling temperature is 15-20 ℃;
the normal-temperature yogurt without additives prepared by the embodiment has the advantages of strong and pure and delicious yogurt taste, mellow and smooth flavor and rich nutrition. The product is slightly separated out of whey after being placed for 5 months at normal temperature, has smooth texture and no bad flavor of nostoc sphaeroides.
Comparative example 1
Normal temperature yogurt without additives was prepared as in example 1, except that the dissolved feed solution was fermented directly without hydration.
Comparative example 2
Normal temperature yogurt without additives was prepared as in example 2, except that the acidity of demulsification after fermentation of nostoc sphaeroids kutz base was controlled to 80 ° T.
Comparative example 3
Normal temperature yogurt without additives was prepared as in example 1, except that the mixed feed was sterilized at 115 ℃.
Sensory evaluation test
Sensory evaluation was performed on the normal temperature yogurts without additives obtained in examples 1 to 5 and comparative examples 1 to 3.
The test mode is as follows: the overall flavor, the sour milk flavor, the smoothness, the viscosity, the bad flavor of the nostoc sphaeroides and other indexes of the product are evaluated in a tasting mode, an anonymous scoring mode is adopted, the full score of each index is 10 points, the higher the score of each index is, the better the indicating effect is, and the number of people to be measured is 350. The results of the tasting were statistically analyzed as in table 1.
It can be seen that the flavor and taste of examples 1 to 5 are superior to those of comparative examples 1 to 3, indicating that the hydration of nostoc sphaeroides, the fermentation acidity of nostoc sphaeroides and the sterilization temperature of the mixing have a direct influence on the taste and flavor.
Wherein, compared with the comparative examples 1 and 2, the example 1 shows that the flavor of the product is better, the smoothness is high and the flavor and the taste of the product are obviously improved by controlling the hydration and the fermentation acidity in the preparation step of the fermented nostoc sphaeroides.
Compared with the comparative example 3, the example 1 shows that the sterilization temperature of the fermented milk and the nostoc sphaeroides fermentation product is too high after the fermented milk and the nostoc sphaeroides fermentation product are mixed, and the product texture is obviously influenced.
Table 1 table of flavor and taste index test data of products
Figure BDA0001908968930000151
Stability analysis
The nostoc powder yogurts obtained in examples 1 to 5 and comparative examples 1 to 3 were allowed to stand at 25 ℃ for 5 months, and the product stability was observed, and the results are shown in table 2.
It can be seen that the nostoc powder yoghurt products obtained in examples 1 to 5 are stable in product system, little in water precipitation, free of precipitate and free of the bad flavor of nostoc after being placed for 5 months at normal temperature.
The comparative example 2 shows water separation and precipitation after being placed for 10 days, the comparative example 1 shows water separation after being placed for 30 days, and the comparative example 3 shows water separation after being placed for 55 days.
Table 2 product stability table
Figure BDA0001908968930000161
Figure BDA0001908968930000171
The product of the invention can be preserved for 5 months at normal temperature, and the sour milk has thick, pure and delicious taste and is mellow and smooth. After the product is placed for 5 months at normal temperature, the product basically has no precipitation, bad flavor and protein coagulation and precipitation phenomena, has stable mouthfeel and longer shelf life. Finally, the product is proved to be reasonable in formula, good in flavor and smooth in mouthfeel.
The nostoc sphaeroides powder is subjected to hydration and separate fermentation treatment, so that not only is the polysaccharide substance of Gao Gexian rice flour effectively dissolved out, but also the bad flavor brought by nostoc sphaeroides is modified, the viscosity and the stability of the product are successfully improved in a green mode, and the normal-temperature yoghourt with clean ingredients is prepared.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of the invention may be made without departing from the scope of the invention.

Claims (14)

1. An additive-free normal temperature yoghourt is characterized by comprising the following components in percentage by weight,
90-98% of fermented milk;
2-10% of fermented nostoc sphaeroids kutz;
wherein the preparation method of the fermented nostoc sphaeroides comprises the following steps: heating water to 40-70 deg.C, adding radix Puerariae Amurensis powder, dissolving, hydrating, sterilizing, adding starter, fermenting, cooling to the end of fermentation, and making into fermented radix Puerariae Amurensis.
2. The non-additive room-temperature yogurt of claim 1, wherein the fermented milk has a fermentation end point acidity of 90-100T °;
and/or the acidity of the fermentation end point of the fermented nostoc sphaeroides is 70-85T degrees.
3. The normal-temperature yogurt without additives as claimed in claim 1, wherein the fermented milk comprises the following raw materials:
910-960 parts of liquid milk;
80-100U/T of leaven;
the fermented nostoc sphaeroids kutz comprises the following raw materials:
50-300 parts of gexian rice flour;
950-700 parts by weight of water;
the leaven is 50-200U/T.
4. The normal temperature yoghurt without additives as claimed in claim 3, wherein the fermented milk and/or the fermented nostoc sphaeroids kutz raw material further comprises 40-90 parts by weight of a sweetener.
5. Normal temperature yogurt without additives as claimed in claim 4,
the liquid milk is at least one of whole milk, skimmed milk, whole goat milk, skimmed goat milk and camel milk;
and/or the sweetener is at least one selected from white granulated sugar, crystalline fructose, fructose and erythritol;
and/or the leavening agent is selected from at least one of lactobacillus delbrueckii subspecies lactis, streptococcus thermophilus, lactobacillus acidophilus, lactobacillus helveticus, lactobacillus plantarum, lactobacillus reuteri, lactobacillus casei and lactobacillus paracasei.
6. The preparation method of the normal-temperature yogurt without additives as claimed in any one of claims 1 to 5, characterized by comprising the following steps:
mixing fermented milk and fermented nostoc sphaeroids kutz at a certain proportion, sterilizing, cooling and filling to obtain the fermented milk.
7. The method for preparing room temperature yogurt without additives as claimed in claim 6,
the preparation steps of the fermented milk are as follows: heating the liquid milk to 45-55 ℃, homogenizing, sterilizing, adding a leaven for fermentation, demulsifying and cooling to the end point of fermentation to obtain the fermented milk.
8. The method for preparing room-temperature yogurt without additives as claimed in claim 7, further comprising adding a sweetener to the step of preparing the fermented milk and/or the step of preparing the fermented nostoc sphaeroides.
9. The preparation method of normal temperature yogurt without additives as claimed in claim 7, wherein the nostoc sphaeroids kutz powder is dissolved by high speed shearing under the conditions of 1000-2000 rpm of rotation speed and 10-20 minutes of time.
10. The method for preparing room temperature yogurt without additives as claimed in any of claims 7 to 9, wherein the hydration treatment conditions in the step of preparing fermented nostoc sphaeroids kutz are stirring rotation speed of 50-150 rpm for 10-30 minutes.
11. Method for preparing normal-temperature yoghurt without additives according to any one of claims 7 to 9, characterized in that the homogenization pressure is 18-22MPa and the temperature is 55-65 ℃.
12. The method for preparing room temperature yogurt without additives as claimed in any one of claims 7 to 9, wherein in the step of preparing the fermented milk, the sterilization temperature is 95-105 ℃ and the time is 3-8min;
and/or, in the preparation of the fermented nostoc sphaeroids kutz, the sterilization temperature is 90-110 ℃, and the time is 3-10min;
and/or mixing the fermented milk and the fermented nostoc sphaeroids kutz according to a ratio, wherein the sterilization is pasteurization, the sterilization temperature is 75-85 ℃, and the sterilization time is 15-20s.
13. The method for preparing normal temperature yogurt without additives as claimed in claim 12, wherein in the step of preparing fermented milk, the fermentation temperature is 37-43 ℃, and the fermentation end point is reached when the fermentation acidity is 70-85 ° T;
and/or in the step of preparing the fermented nostoc sphaeroids kutz, the fermentation temperature is 30-45 ℃, and the fermentation end point is reached when the fermentation acidity is 90-100 DEG T.
14. The preparation method of normal temperature yoghurt without additives as claimed in any one of claims 7 to 9 or 13, wherein the grain size of the gexian rice flour is 20-100 μm.
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