CN111281986B - A kind of18Application of F-fluorine labeled sulfone compound and preparation method of blood pool tracer - Google Patents
A kind of18Application of F-fluorine labeled sulfone compound and preparation method of blood pool tracer Download PDFInfo
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- CN111281986B CN111281986B CN202010065277.8A CN202010065277A CN111281986B CN 111281986 B CN111281986 B CN 111281986B CN 202010065277 A CN202010065277 A CN 202010065277A CN 111281986 B CN111281986 B CN 111281986B
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Abstract
The invention discloses18An application of F-fluorine labeled compound and a preparation method of a blood pool tracer agent belong to the technical field of radiopharmaceuticals and nuclear medicine, and solve the problems that the blood pool tracer agent in the prior art is poor in imaging capability and stability and the imaging process is influenced by glucose metabolism. The invention is shown as formula I18The use of an F-fluorine labelled compound or a salt thereof in the preparation of a blood pool tracer agent,according to the invention18The preparation method of the F fluorine labeled blood pool tracer comprises the steps of adding a buffer solution of a compound shown in the formula I into an RBC buffer solution, incubating, centrifuging, collecting erythrocyte precipitates, adding the buffer solution, and mixing to obtain the F fluorine labeled blood pool tracer. The blood pool tracer agent has obvious stability, has good imaging capability of a heart blood pool, and prolongs the retention time in the heart blood pool; can not penetrate blood brain barrier and has small influence on brain tissue.
Description
Technical Field
The invention belongs to the technical field of radiopharmaceuticals and nuclear medicine, and particularly relates to a radiopharmaceuticals18An application of a F-fluorine labeled sulfone compound in preparation of a blood pool tracer agent and a preparation method of the blood pool tracer agent.
Background
The blood pool tracer is a macromolecular substance marked by radioactive nuclide, and can be completely mixed in blood after intravenous injection to achieve balance, and can be used for scanning or taking pictures by using related instruments to obtain the form of heart large vessel and the imaging of the relation between peripheral tissues and organs thereof, and also can be used for obtaining heart function information by quantitative analysis, and can be used for diagnosing diseases such as coronary heart disease, cardiomyopathy, congenital heart disease, valvular heart disease and the like, and has important significance for monitoring the toxic reaction of drugs to heart, diagnosing chronic intermittent bleeding, particularly lower gastrointestinal bleeding and the like.
With the continuous development of cardiovascular nuclear medicine, new blood pool tracers continue to appear, but have their drawbacks and deficiencies. Such as131The I-labeled Human Serum Albumin (HSA) needs to seal the thyroid three days before imaging, the radiation dose of a patient is large, the nuclide energy is high, and the application of a gamma camera is not facilitated. Applications of the invention99mTc-labeled HSA and Red Blood Cells (RBC) avoid the above problems and improve imaging quality. The blood pool tracer agent used in clinic at present is mainly adopted99mTc-labeled autologous red blood cells predominate. RBC is the visible component with the highest content in blood, is easy to separate and culture in vitro, and has good imaging quality. But do not 99mThe labeling rate of Tc-RBC is often unstable and is greatly influenced by the type and dosage of the applied medicine.99mTc-HSA tends to decrease in blood radioactivity within 15 minutes of injection, while the radioactivity in the liver increases. After 90 minutes, the radioactivity in blood is obviously reduced, the radioactivity of liver and lung is higher, and the imaging of heart blood pool is influenced. Moreover, the imaging agent needs SPECT for detection, and has lower receiving efficiency and poor image quality compared with images obtained by PET/CT imaging. There are also reports related to the use of radioactive nuclides (such as fluorine-18, copper-64, gallium-68, etc.) and evans blue complex (NOTA-EB) labeled HSA as a blood pool tracer, and because the radionuclide needs to be combined with HSA for imaging, the effect is not ideal and the stability is poor. At present, there are18F-FDG labeling of RBC, but because FDG is affected by glucose metabolism, the labeling process requiresFasting and glucose consumption treatments were performed.
Therefore, providing a blood pool tracer with good visualization ability for heart blood pool and good stability is a problem to be solved by those skilled in the art.
Disclosure of Invention
The technical problem solved by the invention is as follows: providing a compound of formula I18The application of the F-fluorine labeled sulfone compound or salt thereof in the preparation of the blood pool tracer agent solves the problems of poor imaging capability and poor stability of the blood pool tracer agent in the prior art.
The invention also provides a method for preparing the blood pool tracer by using the compound shown in the formula I as a raw material.
The technical scheme adopted by the invention is as follows:
the invention is shown as formula I18The use of a F-fluorine labelled compound or a salt thereof in the preparation of a blood pool tracer,
wherein n is 0 to 5.
The invention relates to18The preparation method of F fluorine labeled blood pool tracer comprises adding buffer solution of compound of formula I into RBC buffer solution, incubating, centrifuging, collecting erythrocyte precipitate, adding buffer solution, and mixing to obtain final product18F fluorine labelled blood pool tracers.
In the technical scheme of the invention, the buffer solution of the compound of the formula I is 1-fold PBS solution containing the compound of the formula I, the pH value is 6-8, and preferably the pH value is 7.4;
or/and the RBC buffer solution is 1-fold PBS buffer solution containing RBC, and the pH value is 6-8, preferably the pH value is 7.4.
In the technical scheme of the invention, when the compound in the buffer solution of the compound in the formula I is 1-20 mCi, the red blood cell count in the RBC buffer solution is 0.8^ 9-2 ^ 9.
In the technical scheme of the invention, the incubation condition is incubation for 30-60 min at 35-38 ℃; preferably, the incubation is carried out at 37 ℃ for 45 min.
In the technical scheme of the invention, the centrifugation is performed under a low-temperature condition.
The temperature of the centrifugation is below 20 ℃.
In the technical scheme of the invention, the centrifugal force is 300-400 Xg during centrifugation.
According to the technical scheme, the collected erythrocyte sediment is added into a PBS buffer solution to be repeatedly centrifuged for 2-4 times.
In the technical scheme of the invention, the18The concentration of the red blood cells in the F fluorine labeled blood pool tracer is 3^ 12/L-6 ^ 12/L.
It should be noted that when the blood pool tracer of the invention is used in human, the red blood cells used in the preparation process are human autologous red blood cells; when the blood pool tracer agent is used for animals, the red blood cells used in the preparation process are the red blood cells of the animals.
The preparation method of the RBC buffer solution comprises the following steps: mixing blood with 5-10 times volume of PBS (phosphate buffer solution) containing 2-8 mM EDTA to obtain diluted blood solution, dropping the diluted blood solution on 15mL of leukocyte separation solution (rho is 1.077g/mL), and centrifuging at 20 ℃ at 300-400 Xg for 30 min; eliminating all upper liquid to leave the RBS layer undisturbed; adding 20-30 mL of PBS (containing 5mM EDTA) solution to fill a centrifuge tube, and gently blowing and beating the uniform RBS by a pipette; centrifuging at 300-400 Xg for 20-60 min at 20 ℃; all buffers were removed and the appropriate amount of PBS buffer was added to the cell pellet to obtain RBC buffer.
Compared with the prior art, the invention has the following beneficial effects:
the invention has scientific design and simple method. Compared with the method for labeling by using macrocyclic polyamine chelating groups (such as NOTA) to complex metal radionuclides (gallium-68, copper-64 and technetium-99), the blood pool tracer has obvious stability and advantages by forming stable carbon-fluorine-18-containing chemical bond labeled blood pool tracer.
Blood pool observation and display are carried out after the imaging agent is injected into mammals, and the imaging agent has good heart blood pool imaging capability.
The labeling method is simple, the precursor is easy to obtain, the observation of the heart blood pool of the mammal can be simply and effectively realized, meanwhile, the blood sugar is not influenced (fasting is not needed), and the imaging effect is good; compared with the existing heart blood pool tracer, the tracer prolongs the retention time in the heart blood pool, is mainly excreted by the kidney and the bladder, has higher excretion speed, and reduces the nephrotoxic effect; can not penetrate blood brain barrier and has small influence on brain tissue.
Drawings
FIG. 1 is a HPLC chart of a compound of formula I of the present invention using a radioactivity detector;
FIG. 2 is a HPLC plot of a compound of formula I of the present invention using a UV detector;
FIG. 3 is a micro-PET/CT scan image of normal mice injected with 18F-VS1-RBC for 1 hour;
FIG. 4 is a micro-PET/CT scan image of normal mice injected with 18F-VS1-RBC 5 hours later.
FIG. 5 is a graph of organ biodistribution (X. + -. S) (% ID/g) of 18F-VS1-RBC in normal mice.
Detailed Description
The present invention will be described in further detail with reference to the following examples, which are provided for illustration only and are not intended to limit the scope of the present invention, and any equivalent replacement in the field made in the light of the present disclosure is included in the scope of the present invention.
Example 1
This example discloses compounds of formula I according to the invention18The preparation method of F-VS1 specifically comprises the following steps:
prepared by making accelerator bombs18Capturing F-fluoride ions with an activated QMA column, and then capturing the QMA column18F-fluoride ion is washed to fluoride by using 2.5 percent of TBAB acetonitrile/water mixed solutionDrying with anhydrous acetonitrile for three times in a multifunctional synthesis module reaction tube, and taking out part of the dried anhydrous acetonitrile18A solution of F-TBAF (about 50mCi) was added to a solution of 2mg of the compound of formula II (n ═ 1) in anhydrous acetonitrile (60 μ L), and after heating under sealed conditions at 95 ℃ for 15min, a portion of the reaction solution was removed and subjected to radio-HPLC separation. The chromatographic conditions are as follows: the elution separation was carried out by using ODS C18 column as a column, 0.1% trifluoroacetic acid in acetonitrile as a mobile phase A, 0.1% trifluoroacetic acid in water as a mobile phase B, and a flow rate of 1mL/min, according to the following table.
Time (min) | Mobile phase A (%) | Mobile phase B (%) |
0 | 5 | 95 |
2 | 5 | 95 |
22 | 95 | 5 |
28 | 95 | 5 |
HPLC profiles are shown in FIGS. 1 and 2.
Collecting the collected compound containing formula IEvaporating off acetonitrile from the eluent to obtain the compound of formula I, which is recorded as18F-VS 1. Taking part of a compound of formula I18F-VS1 and standard19F-VS1 solution was analyzed by HPLC mixed injection with consistent peak time.
Example 2
This example discloses the preparation of a blood pool tracer using the compound of formula I prepared in example 1, specifically:
RBC preparation: under isoflurane anesthesia, 200. mu.L of blood was collected after euthanasia of mice due to cervical dislocation, and the blood was mixed with 10-fold volume of PBS containing 5mM EDTA. The diluted blood solution was dropped on 15mL of a leukocyte separation solution (. rho. ═ 1.077g/mL) in a 50mL centrifuge tube, and centrifuged at 1000r/min at 20 ℃ for 30 min. All supernatant liquid was eliminated leaving the RBS layer undisturbed. Add 30mL PBS solution (containing 5mM EDTA) to fill the centrifuge tube and gently pipette the RBCs well. Centrifugation was again carried out at 300Xg1000 r/min for 30min at 20 ℃. All buffers were removed, and 10 to 100. mu.L of a buffer solution of an appropriate amount of PBS was added to the cell pellet as an RBC buffer for use.
The eluent containing the compound of formula I collected in example 1 was evaporated to remove acetonitrile, adjusted to pH 7 to give a 1-fold amount of PBS 18F-VS1 solution was ready for use.
3 to 4mCi18F-VS1 in PBS was added to the above RBC buffer and incubated at 37 ℃ for 45 min. Centrifuging at 20 deg.C for 5min at 300Xg1000 r/min. The erythrocyte pellet was collected and mixed with an appropriate amount of 2ml PBS buffer. PBS buffer addition/centrifugation/red blood cell collection was repeated twice. Finally, 150. mu.L of 1-fold PBS buffer was added and mixed to obtain18F-VS1 labeled blood pool tracer for micro PET/CT imaging.
Example 3
This example discloses a blood pool tracer prepared in example 218Imaging experiments of F-VS1 labeled RBC:
placing a normal mouse on a PET/CT scanning bed, maintaining anesthesia and detecting respiration by using isoflurane-oxygen mixed gas with the volume fraction of 1%, and injecting 50-200 mu Ci to the tail vein of the mouse18F-VS labeling of erythrocytesAnd (4) cell tracer solution. After injection, static imaging of the mice is carried out at different time points, after acquisition and image reconstruction, the myocardium, the blood pool, the liver, the kidney, the brain, the muscle, the bone, the stomach, the small intestine and the brain are sketched on a whole body attenuation correction coronary image by using software Inveon Research Workplace (SIEMENS) provided by a supplier as a region of interest (ROI), and the region of interest (ROI) is obtained by processing data of the Inveon Research Workplace 18F-VS1 labeled erythrocyte tracer solution injected the percent dose per gram of tissue (% ID/g) at this time point.
The results are shown in FIGS. 3 and 4, from the injection18After the F-VS1 marks the erythrocyte tracer solution, the Micro-PET/CT visualizations and the in vivo distribution of normal mice are seen18After the F-VS1 marks the erythrocyte tracer solution for 1 hour, the imaging of the mice can see that the radioactivity distribution of the heart and the blood pool is obvious, the radioactivity distribution of the bladder is high, and the radioactivity distribution of the liver, the kidney, the lung, the bone, the brain and the like is slight; after 5h of injection, the radioactivity distribution in the heart blood pool is still clear, the radioactivity distribution in the stomach and the intestine is increased, the radioactivity distribution in the bladder is reduced, and the radioactivity distribution in the liver and other soft tissues is reduced. Description of the invention18The erythrocyte tracer solution marked by F-VS1 has longer retention time in the heart blood pool, is mainly excreted by the kidney and the bladder, has higher excretion speed, and can reduce the nephrotoxic effect; there is a poor distribution in brain tissue, indicating that it is unable to cross the blood brain barrier.18The organ biodistribution (X. + -. S) (% ID/g) of F-VS1 in normal mice is shown in FIG. 5.
The above-mentioned embodiment is only one of the preferred embodiments of the present invention, and should not be used to limit the scope of the present invention, but all the insubstantial modifications or changes made within the spirit and scope of the main design of the present invention, which still solve the technical problems consistent with the present invention, should be included in the scope of the present invention.
Claims (11)
1. A compound as shown in formula I18The application of the F-fluorine labeled sulfone compound or the salt thereof in the preparation of the blood pool tracer agent,
wherein n is 0 to 5;
the preparation method of the blood pool tracer comprises the following steps: adding buffer solution of compound of formula I into RBC buffer solution, incubating, centrifuging, collecting erythrocyte precipitate, adding buffer solution, and mixing to obtain the final product18F fluorine labelled blood pool tracers.
2. The use of claim 1, wherein the buffer containing the compound of formula I is a 1-fold PBS solution containing the compound of formula I, and has a pH of 6 to 8;
or/and the RBC buffer solution is 1 time PBS buffer solution containing RBC, and the pH value is 6-8.
3. Use according to claim 2, characterized in that the buffer of the compound of formula I has a pH value of 7.4; the pH of the RBC buffer was 7.4.
4. The use of claim 1 or 2, wherein the compound of formula I buffer has a red blood cell count of 0.8^9 to 2^9 when the compound of formula I is 1 to 20 mCi.
5. The use according to claim 1 or 2, wherein the incubation is carried out at 35-38 ℃ for 30-60 min.
6. Use according to claim 5, wherein the incubation conditions are incubation at 37 ℃ for 45 min.
7. Use according to claim 1 or 2, wherein the centrifugation is centrifugation at cryogenic conditions.
8. Use according to claim 7, wherein the temperature of the centrifugation is below 20 ℃.
9. Use according to claim 1 or 2, wherein the centrifugal force during centrifugation is 300 to 400 Xg.
10. The use of claim 9, wherein the collected erythrocyte pellet is added to PBS buffer solution and centrifuged repeatedly for 2-4 times.
11. Use according to claim 1, characterized in that said18The concentration of the red blood cells in the F fluorine marked blood pool tracer is 3^12/L to 6^ 12/L.
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105579434A (en) * | 2013-03-08 | 2016-05-11 | 南加州大学 | Vinylsulfone-based 18F-labeling compositions and methods and uses thereof |
CN110220927A (en) * | 2019-05-28 | 2019-09-10 | 中南大学湘雅医院 | The method for swallowing and being distributed in vivo based on 18F-FDG detection red blood cell |
WO2019204432A2 (en) * | 2018-04-17 | 2019-10-24 | Cornell University | Fluorine-18 labeled compositions and their use in imaging of biological tissue |
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CN105579434A (en) * | 2013-03-08 | 2016-05-11 | 南加州大学 | Vinylsulfone-based 18F-labeling compositions and methods and uses thereof |
WO2019204432A2 (en) * | 2018-04-17 | 2019-10-24 | Cornell University | Fluorine-18 labeled compositions and their use in imaging of biological tissue |
CN110220927A (en) * | 2019-05-28 | 2019-09-10 | 中南大学湘雅医院 | The method for swallowing and being distributed in vivo based on 18F-FDG detection red blood cell |
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Facile Preparation of a Thiol-Reactive 18F-Labeling Agent and Synthesis of 18F-DEG-VS-NT for PET Imaging of a Neurotensin Receptor–Positive Tumor;Zhanhong Wu等;《THE JOURNAL OF NUCLEAR MEDICINE》;20160912;第15卷(第7期);第1178-1184页 * |
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