CN111272705A - Quick detection method for dextrin solubility - Google Patents
Quick detection method for dextrin solubility Download PDFInfo
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- CN111272705A CN111272705A CN202010205485.3A CN202010205485A CN111272705A CN 111272705 A CN111272705 A CN 111272705A CN 202010205485 A CN202010205485 A CN 202010205485A CN 111272705 A CN111272705 A CN 111272705A
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- China
- Prior art keywords
- dextrin
- solubility
- refractive index
- filtrate
- sample
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Links
- 229920001353 Dextrin Polymers 0.000 title claims abstract description 78
- 239000004375 Dextrin Substances 0.000 title claims abstract description 78
- 235000019425 dextrin Nutrition 0.000 title claims abstract description 78
- 238000001514 detection method Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 21
- 238000004519 manufacturing process Methods 0.000 claims abstract description 20
- 239000000706 filtrate Substances 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 238000001914 filtration Methods 0.000 claims description 12
- 239000000047 product Substances 0.000 claims description 11
- 239000012153 distilled water Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 3
- 238000003760 magnetic stirring Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 238000000967 suction filtration Methods 0.000 claims description 2
- 238000011897 real-time detection Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 description 20
- 229920002472 Starch Polymers 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 239000011259 mixed solution Substances 0.000 description 5
- 235000019698 starch Nutrition 0.000 description 5
- 239000008107 starch Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Abstract
The invention discloses a quick detection method of dextrin solubility, which comprises the steps of establishing a numerical value relation between the dextrin solubility and a refractive index measured by a refractive index detector by using dextrin standard substances with different solubilities, and measuring the dextrin solubility by measuring the refractive index of a sample. The invention has simple process, low cost, high detection speed and high accuracy, and can be used for real-time detection in the production process to guide the production to make process adjustment in time.
Description
Technical Field
The invention belongs to the technical field of food and medicine detection, and particularly relates to a method for rapidly detecting dextrin solubility.
Background
Dextrin is an incompletely hydrolyzed product generated by starch after acid heat treatment or α amylase action, and the components and the performance of the dextrin are greatly different with the hydrolysis degree of starch.
The application and product performance of dextrin products are mainly influenced by dextrin solubility (the dextrin solubility and the dextrin conversion degree are in a positive correlation, so that the dextrin conversion degree can be directly reflected by measuring the solubility) indexes, the dextrin conversion process is a continuous conversion process from low to high, the conversion rate is high, the solubilities of different starch raw materials are different under the same reaction condition, and the conditions of excessive roasting or insufficient conversion degree are easy to occur in the actual production of dextrin. The conventional simple detection method comprises the methods of detecting the viscosity of dextrin, judging the color change of the dextrin by an iodine dyeing method and the like, has complicated steps, is influenced by the proficiency and experience of an operator, has low result accuracy and cannot timely and quickly guide the production to control the reaction degree of the dextrin. And the standard dextrin solubility determination method is too long in time, is only suitable for finished product detection in a laboratory, and cannot effectively guide actual production operation.
Therefore, how long starch is converted into dextrin by roasting needs a rapid analysis method for production control. According to the principle that the hydrolysis process of dextrin is that the molecular chain of starch is thermally hydrolyzed and shortened to micromolecular dextrin and partial reducing sugar, the micromolecular dextrin and the reducing sugar are easily dissolved in water, the concentration of the dextrin and the reducing sugar dissolved in the water is different, and the generated refractive index is also different, the method for quickly judging the dextrin solubility by detecting the refractive index of the solution of the dextrin dissolved in the water is developed by combining the inspiration of a dextrin solubility detection method. Provides an innovative and breakthrough detection method for accurately controlling the dextrin conversion degree in the production process.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the method for rapidly detecting the dextrin solubility is high in detection speed and high in accuracy, and can be used for rapidly detecting and judging the dextrin solubility in the production process and guiding production to carry out operation adjustment.
In order to solve the technical problems, the technical scheme of the invention is as follows:
a method for rapidly detecting dextrin solubility comprises the following steps:
a. putting dextrin samples of different solubility standard products into different triangular flasks respectively, adding 5-10 times of distilled water with the temperature of 25 ℃ in mass, and shaking up until the dextrin is fully dissolved;
b. pouring the mixed liquid obtained in the step a into a funnel respectively, and filtering to obtain filtrate;
c. respectively sucking 1-2 ml of the filtrate obtained in the step b, and measuring the refractive index of each filtrate by using a refractive index detector;
d. c, establishing a data corresponding relation between dextrin solubility and refractive index measured by a refractive index detector according to the data obtained in the step c;
e. and d, measuring the refractive index of the dextrin sample in actual production, namely obtaining the dextrin solubility through the corresponding numerical relation obtained in the step d.
Preferably, the amount of distilled water at 25 ℃ added in the step a is 6 times of the amount of the sample.
Preferably, the shaking in step a may be any dispersing means capable of performing a uniform mixing function, such as magnetic stirring, rotary stirring, and a shaker.
Preferably, the filtration in the funnel in the step b can adopt any solid-liquid separation mode such as a suction filtration mode, a natural funnel filtration mode and the like.
Preferably, the refractive index detector in step c is a digital abbe refractometer.
Due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the method comprises the steps of detecting the filtrate refractive index of dextrin samples with different conversion degrees (solubilities) to establish corresponding numerical value relations between different dextrin solubilities and the refractive index; the solubility of the dextrin sample is rapidly determined according to the corresponding relation, the method is suitable for rapidly detecting and obtaining the data of the reaction degree of the dextrin in the industrial production of the dextrin, is convenient for timely adjustment of production, and produces a stable and uniform dextrin product;
2. the invention has simple process, low cost, no use of chemical reagent and rapid detection, and is suitable for the guidance control of dextrin solubility in all dry-process dextrin production lines.
In a word, the invention overcomes the defect that the dextrin solubility is not detected quickly in the prior art, has high detection speed and high accuracy, and can be used for real-time detection in the production process to guide the production to make process adjustment in time.
Detailed Description
The invention is further illustrated by the following examples.
Example oneRelationship between dextrin solubility and refractive index of Abbe refractometer
a. Respectively taking 25g of dextrin standard samples with different solubilities, respectively putting the dextrin standard samples into 250ml dry clean volumetric flasks, and introducing purified water at 40 ℃ for constant volume; fully shaking to melt, keeping the mixture in a 40 ℃ water bath kettle for 1 hour, filtering the mixture by using medium-speed filter paper, taking 10ml of filtrate, putting the filtrate into a drying bottle in a balance (the weight of the drying bottle is W1), drying the filtrate by using the water bath kettle, putting the dried filtrate into a 105 ℃ drying oven for 2 hours, cooling the dried filtrate to room temperature, weighing the dried filtrate, and recording the weight as W2, wherein the dextrin solubility (solubility) is (W2-W1)/(1-water); calculating the solubility of dextrin;
b. respectively taking 4g of dextrin samples of dextrin standard samples with different solubilities, respectively placing the dextrin samples into different triangular flasks, adding 25ml of distilled water with the mass of 25 ℃, and shaking up until the dextrin is fully dissolved; pouring the obtained mixed solution into a funnel respectively, and filtering to obtain filtrate; respectively sucking 1-2 ml of the filtrate and dripping the filtrate on a detection mirror surface of an Abbe refractometer, and measuring the refractive index of each filtrate;
the data integer correspondence of dextrin solubility and refractive index of Abbe refractometer is as follows:
| solubility in water | Refractive index of Abbe refractometer |
| 0.1 | 1.0±0.1 |
| 0.2 | 2.0±0.1 |
| 0.3 | 3.0±0.1 |
| 0.4 | 3.9±0.1 |
| 0.5 | 4.7±0.1 |
| 0.6 | 5.6±0.2 |
| 0.7 | 7.5±0.2 |
| 0.8 | 9.5±0.2 |
| 0.9 | 11±0.5 |
Example two
a. Quickly taking 4g of a reacted sample from a production line reaction kettle, placing the sample in a triangular flask, adding 25ml of 25 ℃ distilled water, and shaking up until the dextrin is fully dissolved;
b. pouring the obtained mixed solution into a funnel for filtering, and reserving filtrate;
c. and (3) respectively sucking 1-2 ml of filtrate and dripping the filtrate on a detection mirror surface of an Abbe refractometer, measuring the refractive index of the filtrate to be 2.2, obtaining that the solubility of a product of dextrin reaction is 0.21, and verifying that the solubility of the sample is 0.211 by using an experimental method, wherein the result is approximate.
EXAMPLE III
a. Weighing 8g of a reacted sample from a production line reaction kettle, placing the sample in a triangular flask, adding 50ml of 25 ℃ distilled water, and shaking up until the dextrin is fully dissolved;
b. pouring the obtained mixed solution into a funnel for filtering, and reserving filtrate;
c. and (3) respectively sucking 1-2 ml of filtrate and dripping the filtrate on a detection mirror surface of an Abbe refractometer, measuring the refractive index of the filtrate to be 3.2, checking a table to obtain that the solubility of a product of dextrin reaction is 0.34, and verifying that the solubility of the sample is 0.342 by using an experimental method, wherein the results are similar.
Example four
a. Quickly taking 4g of a reacted sample from a production line reaction kettle, placing the sample in a triangular flask, adding 25ml of 25 ℃ distilled water, and shaking up until the dextrin is fully dissolved;
b. pouring the obtained mixed solution into a funnel for filtering, and reserving filtrate;
c. and (3) respectively sucking 1-2 ml of filtrate and dripping the filtrate on a detection mirror surface of an Abbe refractometer, measuring the refractive index of the filtrate to be 7.7, checking a table to obtain that the solubility of a product of dextrin reaction is 0.73, and verifying that the solubility of the sample is 0.731 by using an experimental method, wherein the results are similar.
EXAMPLE five
a. Quickly taking 4g of a reacted sample from a production line reaction kettle, placing the sample in a triangular flask, adding 25ml of 25 ℃ distilled water, and shaking up until the dextrin is fully dissolved;
b. pouring the obtained mixed solution into a funnel for filtering, and reserving filtrate;
c. and (3) respectively sucking 1-2 ml of filtrate and dripping the filtrate on a detection mirror surface of an Abbe refractometer, measuring the refractive index of the filtrate to be 11.5, checking a table to obtain that the solubility of a product of dextrin reaction is 0.96, and verifying that the solubility of the sample is 0.959 by using an experimental method, wherein the results are similar.
As can be seen from the examples 1, 2, 3, 4 and 5, the refractive index is measured by the Abbe refractometer to establish the numerical value corresponding relation with the dextrin solubility, the refractive index of dextrin filtrate can be rapidly detected in production, the dextrin solubility numerical value can be rapidly corresponding, and the dextrin conversion degree can be known.
It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
Claims (5)
1. A method for rapidly detecting dextrin solubility is characterized by comprising the following steps:
a. putting dextrin samples of different solubility standard products into different triangular flasks respectively, adding 5-10 times of distilled water with the temperature of 25 ℃ in mass, and shaking up until the dextrin is fully dissolved;
b. pouring the mixed liquid obtained in the step a into a funnel respectively, and filtering to obtain filtrate;
c. respectively sucking 1-2 ml of the filtrate obtained in the step b, and measuring the refractive index of each filtrate by using a refractive index detector;
d. c, establishing a data corresponding relation between dextrin solubility and refractive index measured by a refractive index detector according to the data obtained in the step c;
e. and d, measuring the refractive index of the dextrin sample in actual production, and obtaining the dextrin solubility through the corresponding numerical value relationship obtained in the step d.
2. The method for rapid detection of dextrin solubility according to claim 1, characterized in that: the amount of distilled water at 25 ℃ added in the step a is 6 times of the amount of the sample.
3. The method for rapid detection of dextrin solubility according to claim 1, characterized in that: the shaking up mode in the step a can adopt magnetic stirring, rotary stirring or an oscillator to mix and shake up.
4. The method for rapid detection of dextrin solubility according to claim 1, characterized in that: and c, filtering in the funnel in the step b can be carried out by adopting a suction filtration mode and a natural funnel filtration mode.
5. The method for rapid detection of dextrin solubility according to claim 1, characterized in that: and c, the refractive index detector in the step c is a digital Abbe refractometer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010205485.3A CN111272705A (en) | 2020-03-23 | 2020-03-23 | Quick detection method for dextrin solubility |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010205485.3A CN111272705A (en) | 2020-03-23 | 2020-03-23 | Quick detection method for dextrin solubility |
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| Publication Number | Publication Date |
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| CN111272705A true CN111272705A (en) | 2020-06-12 |
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| CN202010205485.3A Pending CN111272705A (en) | 2020-03-23 | 2020-03-23 | Quick detection method for dextrin solubility |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2765180Y (en) * | 2004-08-03 | 2006-03-15 | 中国海洋大学 | A device for direct measurement of crude oil refractive index |
| CN101123883A (en) * | 2004-02-23 | 2008-02-13 | 金克克国际有限公司 | A method for the preparation of a high-purity rice protein concentrate |
| JP2008303175A (en) * | 2007-06-07 | 2008-12-18 | Otsuka Pharmaceut Co Ltd | Gi value-reducing agent and method for producing foodstuff reduced with gi value |
| US20100305872A1 (en) * | 2009-05-31 | 2010-12-02 | University Of Kuwait | Apparatus and Method for Measuring the Properties of Petroleum Factions and Pure Hydrocarbon Liquids by Light Refraction |
| CN102770501A (en) * | 2010-01-19 | 2012-11-07 | 克鲁技术公司 | Improved adhesive composition |
| CN103890121A (en) * | 2011-11-09 | 2014-06-25 | 英派尔科技开发有限公司 | Dendritic starch-based dextrin adhesives |
| CN104379705A (en) * | 2012-06-22 | 2015-02-25 | 贝克休斯公司 | Process for predicting the stability of crude oil and employing same in transporting and/or refining the crude oil |
| CN104520333A (en) * | 2012-08-09 | 2015-04-15 | 卡吉尔公司 | Process for starch liquefaction |
| CN206069736U (en) * | 2016-08-28 | 2017-04-05 | 山东昆达生物科技有限公司 | A kind of potassium sorbate precision production system |
| CN109813680A (en) * | 2017-11-19 | 2019-05-28 | 湖南轻创科技有限公司 | A kind of entity and its application for measurement |
-
2020
- 2020-03-23 CN CN202010205485.3A patent/CN111272705A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101123883A (en) * | 2004-02-23 | 2008-02-13 | 金克克国际有限公司 | A method for the preparation of a high-purity rice protein concentrate |
| CN2765180Y (en) * | 2004-08-03 | 2006-03-15 | 中国海洋大学 | A device for direct measurement of crude oil refractive index |
| JP2008303175A (en) * | 2007-06-07 | 2008-12-18 | Otsuka Pharmaceut Co Ltd | Gi value-reducing agent and method for producing foodstuff reduced with gi value |
| US20100305872A1 (en) * | 2009-05-31 | 2010-12-02 | University Of Kuwait | Apparatus and Method for Measuring the Properties of Petroleum Factions and Pure Hydrocarbon Liquids by Light Refraction |
| CN102770501A (en) * | 2010-01-19 | 2012-11-07 | 克鲁技术公司 | Improved adhesive composition |
| CN103890121A (en) * | 2011-11-09 | 2014-06-25 | 英派尔科技开发有限公司 | Dendritic starch-based dextrin adhesives |
| CN104379705A (en) * | 2012-06-22 | 2015-02-25 | 贝克休斯公司 | Process for predicting the stability of crude oil and employing same in transporting and/or refining the crude oil |
| CN104520333A (en) * | 2012-08-09 | 2015-04-15 | 卡吉尔公司 | Process for starch liquefaction |
| CN206069736U (en) * | 2016-08-28 | 2017-04-05 | 山东昆达生物科技有限公司 | A kind of potassium sorbate precision production system |
| CN109813680A (en) * | 2017-11-19 | 2019-05-28 | 湖南轻创科技有限公司 | A kind of entity and its application for measurement |
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Application publication date: 20200612 |