CN111270520B - Flexible fabric capable of efficiently emitting light to detect bacteria and preparation method and application thereof - Google Patents
Flexible fabric capable of efficiently emitting light to detect bacteria and preparation method and application thereof Download PDFInfo
- Publication number
- CN111270520B CN111270520B CN202010052222.3A CN202010052222A CN111270520B CN 111270520 B CN111270520 B CN 111270520B CN 202010052222 A CN202010052222 A CN 202010052222A CN 111270520 B CN111270520 B CN 111270520B
- Authority
- CN
- China
- Prior art keywords
- fabric
- solution
- hours
- flexible
- cotton fabric
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000004744 fabric Substances 0.000 title claims abstract description 117
- 241000894006 Bacteria Species 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 241000588724 Escherichia coli Species 0.000 claims abstract description 23
- 239000002105 nanoparticle Substances 0.000 claims abstract description 21
- 241000191967 Staphylococcus aureus Species 0.000 claims abstract description 12
- 229920000867 polyelectrolyte Polymers 0.000 claims abstract description 11
- 238000010791 quenching Methods 0.000 claims abstract description 10
- 230000000171 quenching effect Effects 0.000 claims abstract description 10
- 229920002385 Sodium hyaluronate Polymers 0.000 claims abstract description 7
- 229940010747 sodium hyaluronate Drugs 0.000 claims abstract description 7
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 7
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229920001287 Chondroitin sulfate Polymers 0.000 claims abstract description 5
- 229940059329 chondroitin sulfate Drugs 0.000 claims abstract description 5
- 229920000669 heparin Polymers 0.000 claims abstract description 5
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 claims abstract description 5
- 229960001008 heparin sodium Drugs 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 91
- 229920000742 Cotton Polymers 0.000 claims description 48
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N EtOH Substances CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 32
- 238000006243 chemical reaction Methods 0.000 claims description 26
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- 229920000297 Rayon Polymers 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 15
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 12
- 229910016644 EuCl3 Inorganic materials 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 9
- 239000011259 mixed solution Substances 0.000 claims description 9
- YNPNZTXNASCQKK-UHFFFAOYSA-N Phenanthrene Natural products C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 claims description 8
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 8
- 229920000728 polyester Polymers 0.000 claims description 8
- WRMNZCZEMHIOCP-UHFFFAOYSA-N 2-phenylethanol Chemical compound OCCC1=CC=CC=C1 WRMNZCZEMHIOCP-UHFFFAOYSA-N 0.000 claims description 7
- 239000007974 sodium acetate buffer Substances 0.000 claims description 7
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 238000002189 fluorescence spectrum Methods 0.000 claims description 6
- WXZMFSXDPGVJKK-UHFFFAOYSA-N pentaerythritol Chemical compound OCC(CO)(CO)CO WXZMFSXDPGVJKK-UHFFFAOYSA-N 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- RLHGFJMGWQXPBW-UHFFFAOYSA-N 2-hydroxy-3-(1h-imidazol-5-ylmethyl)benzamide Chemical compound NC(=O)C1=CC=CC(CC=2NC=NC=2)=C1O RLHGFJMGWQXPBW-UHFFFAOYSA-N 0.000 claims description 5
- JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 239000003431 cross linking reagent Substances 0.000 claims description 4
- LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 claims description 4
- 230000007935 neutral effect Effects 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims description 4
- PXRKCOCTEMYUEG-UHFFFAOYSA-N 5-aminoisoindole-1,3-dione Chemical compound NC1=CC=C2C(=O)NC(=O)C2=C1 PXRKCOCTEMYUEG-UHFFFAOYSA-N 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 238000004020 luminiscence type Methods 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims description 2
- NNMXSTWQJRPBJZ-UHFFFAOYSA-K europium(iii) chloride Chemical compound Cl[Eu](Cl)Cl NNMXSTWQJRPBJZ-UHFFFAOYSA-K 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 229910021645 metal ion Inorganic materials 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 description 17
- 229910052693 Europium Inorganic materials 0.000 description 10
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 10
- 239000011541 reaction mixture Substances 0.000 description 10
- 239000007864 aqueous solution Substances 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 239000000835 fiber Substances 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000000295 emission spectrum Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000000695 excitation spectrum Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
Images
Classifications
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/50—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with organometallic compounds; with organic compounds containing boron, silicon, selenium or tellurium atoms
- D06M13/503—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with organometallic compounds; with organic compounds containing boron, silicon, selenium or tellurium atoms without bond between a carbon atom and a metal or a boron, silicon, selenium or tellurium atom
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M11/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising
- D06M11/32—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with oxygen, ozone, ozonides, oxides, hydroxides or percompounds; Salts derived from anions with an amphoteric element-oxygen bond
- D06M11/36—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with oxygen, ozone, ozonides, oxides, hydroxides or percompounds; Salts derived from anions with an amphoteric element-oxygen bond with oxides, hydroxides or mixed oxides; with salts derived from anions with an amphoteric element-oxygen bond
- D06M11/38—Oxides or hydroxides of elements of Groups 1 or 11 of the Periodic Table
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
- D06M13/144—Alcohols; Metal alcoholates
- D06M13/148—Polyalcohols, e.g. glycerol or glucose
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
- D06M13/184—Carboxylic acids; Anhydrides, halides or salts thereof
- D06M13/192—Polycarboxylic acids; Anhydrides, halides or salts thereof
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
- D06M13/184—Carboxylic acids; Anhydrides, halides or salts thereof
- D06M13/207—Substituted carboxylic acids, e.g. by hydroxy or keto groups; Anhydrides, halides or salts thereof
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
- D06M15/01—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with natural macromolecular compounds or derivatives thereof
- D06M15/03—Polysaccharides or derivatives thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/04—Vegetal fibres
- D06M2101/06—Vegetal fibres cellulosic
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/16—Synthetic fibres, other than mineral fibres
- D06M2101/30—Synthetic polymers consisting of macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- D06M2101/32—Polyesters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Landscapes
- Textile Engineering (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Emergency Medicine (AREA)
- Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
Abstract
The invention discloses a flexible fabric capable of efficiently emitting light to detect bacteria, and a preparation method and application thereof. Grafting hybrid luminescent nanoparticles on the surface of a fabric through a covalent bond, wherein the hybrid luminescent nanoparticles are obtained by embedding a metal ion complex with high-efficiency luminescent property by using natural biological polyelectrolyte with negative charges, such as heparin sodium, sodium hyaluronate, chondroitin sulfate and the like; after the luminous fabric is co-cultured with escherichia coli (e.coli) and staphylococcus aureus (s.aureus) for 12-24 hours, the existence of bacteria can be detected through the quenching of the fluorescence signal of the luminous fabric.
Description
Technical Field
The invention relates to a flexible fabric capable of efficiently emitting light to detect bacteria, and a preparation method and application thereof.
Background
The fabric has the advantages of high strength, soft hand feeling, strong skin affinity and the like, so the fabric is widely applied to daily life. However, fabrics are susceptible to bacterial damage. Bacteria can live on the fabric and spread disease. The mechanical properties and certain functions of the fabric may be impaired. Bacterial infections are becoming increasingly difficult to treat due to rising antibiotic resistance and a stagnation in antibiotic development and approval. It is very urgent to develop a functional fabric for early detection of the presence of bacteria.
Disclosure of Invention
The invention aims to provide a flexible fabric capable of efficiently emitting light to detect bacteria, and a preparation method and application thereof. After the luminous fabric is co-cultured with escherichia coli (e.coli) and staphylococcus aureus (s.aureus) for 12-24 hours, the existence of bacteria can be detected through the quenching of the fluorescence signal of the luminous fabric. The invention creatively grafts the hybrid luminescent nano-particles on the surface of the fabric through covalent bonds. The hybridized luminous nano particle is obtained by embedding a metal ion complex with high-efficiency luminous characteristic by using natural biological polyelectrolyte with negative charges, such as heparin sodium, sodium hyaluronate, chondroitin sulfate and the like.
A preparation method of a flexible fabric capable of efficiently emitting light to detect bacteria comprises the following steps:
(1) preparing a solution: preparing a polyelectrolyte solution by using an acetic acid-sodium acetate buffer solution with the pH value of 0.1M being 5; preparation of EuCl with ethanol3Solutions, tta solutions and phen solutions;
(2) preparing the hybrid luminescent nano particles: to EuCl3Dripping tta solution into the solution, and stirring 1 by a magnetic stirrerAdjusting the pH of the reaction solution to 6-8 after 2 hours, then dropwise adding a phen solution into the reaction solution, stirring the reaction solution for 1-5 hours, then adding a polyelectrolyte solution with negative charges into the reaction solution, and stirring the reaction solution for 1-5 hours to obtain hybrid luminescent nanoparticles;
(3) preparing a flexible luminous cotton fabric: treating the fabric in the reaction solution for 2-10 hours at the temperature of room temperature to 90 ℃, then adding the prepared hybrid luminescent nanoparticles into the reaction solution, keeping the temperature of room temperature to 90 ℃, and treating for 2-10 hours to obtain the flexible fabric; the reaction solution consists of a non-formaldehyde crosslinking agent and a penetrant.
The preparation method, EuCl3The concentration of the ethanol solution is 0.01 mol/L; the concentration of the tta ethanol solution is 0.03 mol/L; the concentration of the phen ethanol solution is 0.01 mol/L.
The preparation method, EuCl3The amount ratio of substances tta and phen EuCl3：tta：phen＝1：3：1。
In the preparation method, the polyelectrolyte solution is one or two of natural biological polyelectrolytes including heparin sodium, sodium hyaluronate and chondroitin sulfate.
The preparation method is characterized in that the stirring speed is 800rpm-1500rpm when the magnetic stirrer is used for stirring.
In the preparation method, the fabric is polyester cotton cloth, pure cotton fabric or viscose fabric; for the pure cotton fabric, before the reaction solution treatment in the step (3), the pure cotton fabric is firstly put into NaOH solution, and then the JFC penetrating agent is dripped and fully mixed; heating the mixed solution to boiling, and keeping boiling for 30 minutes; taking out the treated cotton fabric, washing the cotton fabric for a plurality of times by using hot water, and finally washing the cotton fabric until the cotton fabric is neutral by using cold water; the concentration of the JFC penetrant is 1 g/L; the concentration of the NaOH solution is 10 g/L.
According to the preparation method, the non-formaldehyde crosslinking agent is one of 1,2,3, 4-butanetetracarboxylic acid (BTCA), Citric Acid (CA), Pentaerythritol (PER), Maleic Acid (MA) and Itaconic Acid (IA); the penetrating agent is one of F-121, T99 and JFC.
According to the preparation method, the bath ratio of the fabric to the reaction liquid in the reaction process is 1: 50; the concentration of the penetrant during the reaction is 1 g/L.
The flexible fabric capable of efficiently emitting light and detecting bacteria is prepared according to any one of the preparation methods.
The application of the flexible fabric is used for detecting bacteria: and (2) respectively culturing the flexible luminous fabric with escherichia coli (E.coli) and staphylococcus aureus (S.aureus) for 12-24 hours at room temperature to 40 ℃, testing the fluorescence spectra of the flexible luminous fabric before and after bacterial culture, and detecting the existence of bacteria through the quenching of the fluorescence signal of the luminous fabric.
The fluorescence spectra of the flexible luminous fabric before and after bacterial culture are tested, and the fluorescence quenching of the flexible luminous fabric can be found. Therefore, the flexible luminous fabric can detect the existence of escherichia coli and staphylococcus aureus through high-efficiency luminescence, and can be widely applied to medical and daily environments.
Drawings
FIG. 1 shows the excitation and emission spectra of the hybrid luminescent nanoparticles.
Fig. 2 is a fluorescence emission spectrum of the flexible luminous fabric before and after the flexible luminous fabric and escherichia coli (e.coli) are co-cultured for 12-24 hours.
Fig. 3 is a fluorescence emission spectrum of the flexible luminous fabric after the flexible luminous fabric is co-cultured with staphylococcus aureus (s.
FIG. 4 is a flow chart of a method for preparing a flexible fabric capable of detecting bacteria by high-efficiency luminescence.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Example 1
To 1mL of 0.01mol/L EuCl31mL of 0.03mol/L tta ethanol solution was added to the ethanol solution, and the mixture was stirred at room temperature for 1.5 hours. After the pH of the reaction mixture was adjusted to 7 to 8, 1mL of 0.01mol/L phen ethanol solution was added to the reaction mixture, and the mixture was stirred for 3 hours to obtain a europium complex solution.
4mL of a 10mg/mL sodium hyaluronate solution (prepared from 0.1mol/L acetic acid-sodium acetate buffer solution with pH 5 as a solvent) was added to the obtained europium complex solution, and stirred at room temperature for 4 hours to obtain hybrid luminescent nanoparticles (shown in fig. 1, excitation and emission spectra of the hybrid luminescent nanoparticles).
Cotton fabric was placed in a beaker with NaOH solution (10g/L) and then JFC penetrant (1g/L) was added dropwise and mixed well. The mixture was heated to boiling and kept boiling for 30 minutes. And taking out the treated cotton fabric, washing the cotton fabric for a plurality of times by using hot water, and finally washing the cotton fabric until the cotton fabric is neutral by using cold water, so that the influence of residual NaOH on subsequent experiments is avoided.
The reaction solution consisted of a mixture of 61.6mL of Pentaerythritol (PER) aqueous solution (10g/L) heated to 60 ℃ and 5 drops of JFC penetrant (1g/L), 1.232g of the above cotton fabric was immersed in it (bath ratio 50: 1) to graft the surface of the cotton fabric, after stirring for 4 hours, 40mL of hybrid luminescent nanoparticles were added to it and the treatment continued at 60 ℃ for 4 hours to graft the hybrid luminescent particles to the cotton fabric fibers. The treated cotton fabric was taken out, washed several times with hot water and then several times with cold water. And taking out the cotton fabric and drying to constant weight to obtain the flexible luminous cotton fabric.
The method comprises the steps of respectively culturing the flexible luminous cotton fabric with escherichia coli (E.coli) and staphylococcus aureus (S.aureus) for 12-24 hours at room temperature to 40 ℃, and then testing fluorescence spectra of the flexible luminous cotton fabric before and after bacterial culture (shown in a figure 2, a fluorescence emission spectrogram of the flexible luminous fabric before and after the flexible luminous fabric and the escherichia coli (E.coli) are cultured together for 12-24 hours and a figure 3, a fluorescence emission spectrogram of the flexible luminous fabric before and after the flexible luminous fabric and the staphylococcus aureus (S.aureus) are cultured together for 12-24 hours), wherein the existence of bacteria can be detected through fluorescence signal quenching of the flexible luminous cotton fabric.
Example 2
To 1mL of 0.01mol/L EuCl31mL of 0.03mol/L tta ethanol solution was added to the ethanol solution, and the mixture was stirred at room temperature for 1.5 hours. After the pH of the reaction mixture was adjusted to 7 to 8, 1mL of 0.01mol/L phen ethanol solution was added to the reaction mixture, and the mixture was stirred for 3 hours to obtain a europium complex solution.
4mL of a 10mg/mL heparin sodium solution (prepared from 0.1mol/L acetic acid-sodium acetate buffer solution with pH 5 as a solvent) was added to the europium complex solution obtained above, and the mixture was stirred at room temperature for 4 hours to obtain hybrid luminescent nanoparticles.
The reaction solution consisted of 31.4mL of a mixed solution of 1,2,3, 4-butanetetracarboxylic acid (BTCA) aqueous solution (10g/L) heated to 60 ℃ and 2 drops of F-121 penetrant (1g/L), 0.627g of the viscose fabric was immersed therein (bath ratio 50: 1) to graft-pretreat the surface of the viscose fabric, 20mL of hybrid luminescent nanoparticles were added thereto after stirring for 4 hours, and treatment was continued at 60 ℃ for 4 hours to graft the hybrid luminescent particles onto the viscose fabric fibers. The treated viscose fabric was taken out, washed several times with hot water and then several times with cold water. And taking out the viscose fabric and drying to constant weight to obtain the flexible luminous viscose fabric. After the flexible luminous viscose fabric is respectively cultured with escherichia coli (E.coli) and staphylococcus aureus (S.aureus), the existence of bacteria can be detected through the quenching of the fluorescence signal of the luminous viscose fabric.
Example 3
To 1ml of 0.01mol/L EuCl31mL of 0.03mol/L tta ethanol solution was added to the ethanol solution, and the mixture was stirred at room temperature for 1.5 hours. After the pH of the reaction mixture was adjusted to 7 to 8, 1mL of 0.01mol/L phen ethanol solution was added to the reaction mixture, and the mixture was stirred for 3 hours to obtain a europium complex solution.
4mL of a 10mg/mL sodium hyaluronate solution (prepared from 0.1mol/L acetic acid-sodium acetate buffer solution with pH 5 as a solvent) was added to the europium complex solution obtained above, and the mixture was stirred at room temperature for 4 hours to obtain hybrid luminescent nanoparticles.
The reaction solution consists of a mixed solution of 41.2mL of 1,2,3, 4-butanetetracarboxylic acid (BTCA) aqueous solution (10g/L) heated to 80 ℃ and 4 drops of F-121 penetrant (1g/L), 0.824g of viscose fabric is immersed in the mixed solution (bath ratio is 50: 1) to carry out grafting pretreatment on the surface of the viscose fabric, 25mL of hybrid luminescent nano-particles are added in the mixed solution after stirring for 4 hours, and the mixed solution is continuously treated at 80 ℃ for 4 hours to graft the hybrid luminescent particles on the viscose fabric fibers. The treated viscose fabric was taken out, washed several times with hot water and then several times with cold water. And taking out the viscose fabric and drying to constant weight to obtain the flexible luminous viscose fabric. After the flexible luminous viscose fabric is respectively cultured with escherichia coli (E.coli) and staphylococcus aureus (S.aureus), the existence of bacteria can be detected through the quenching of the fluorescence signal of the luminous viscose fabric.
Example 4
To 1mL of 0.01mol/L EuCl31mL of 0.03mol/L tta ethanol solution was added to the ethanol solution, and the mixture was stirred at room temperature for 1.5 hours. After the pH of the reaction mixture was adjusted to 7 to 8, 1mL of 0.01mol/L phen ethanol solution was added to the reaction mixture, and the mixture was stirred for 3 hours to obtain a europium complex solution.
4mL of a 10mg/mL chondroitin sulfate solution (prepared from 0.1mol/L acetic acid-sodium acetate buffer solution with pH of 5 as a solvent) was added to the obtained europium complex solution, and the mixture was stirred at room temperature for 4 hours to obtain hybrid luminescent nanoparticles.
The reaction solution consisted of a mixed solution of 42.6mL of Citric Acid (CA) aqueous solution (10g/L) heated to 60 ℃ and 4 drops of F-121 penetrant (1g/L), 0.852g of polyester cotton cloth was immersed therein (bath ratio 50: 1) to perform a grafting pretreatment on the surface of the polyester cotton fabric, after stirring for 4 hours, 28mL of hybrid luminescent nanoparticles were added thereto, and treatment was continued at 60 ℃ for 4 hours to graft the hybrid luminescent particles onto the polyester cotton fabric fibers. The treated polyester cotton cloth was taken out, washed several times with hot water and then several times with cold water. And taking out the polyester cotton cloth and drying to constant weight to obtain the flexible luminous polyester cotton cloth. After the flexible luminescent polyester-cotton fabric is respectively cultured with escherichia coli (E.coli) and staphylococcus aureus (S.aureus), the existence of bacteria can be detected through the quenching of the fluorescence signal of the luminescent polyester-cotton fabric.
Example 5
To 1mL of 0.01mol/L EuCl31mL of 0.03mol/L tta ethanol solution was added to the ethanol solution, and the mixture was stirred at room temperature for 1.5 hours. After the pH of the reaction mixture was adjusted to 7 to 8, 1mL of 0.01mol/L phen ethanol solution was added to the reaction mixture, and the mixture was stirred for 3 hours to obtain a europium complex solution.
4mL of a 10mg/mL sodium hyaluronate solution (prepared from 0.1mol/L acetic acid-sodium acetate buffer solution with pH 5 as a solvent) was added to the europium complex solution obtained above, and the mixture was stirred at room temperature for 4 hours to obtain hybrid luminescent nanoparticles.
Cotton fabric was placed in a beaker with NaOH solution (10g/L) and then JFC penetrant (1g/L) was added dropwise and mixed well. The mixture was heated to boiling and kept boiling for 30 minutes. The treated cotton fabric was taken out, washed several times with hot water and finally washed with cold water until neutral.
The reaction solution consisted of 53.7mL of a mixed solution of 1,2,3, 4-butanetetracarboxylic acid (BTCA) aqueous solution (10g/L) heated to 80 ℃ and 5 drops of F-121 penetrant (1g/L), 1.074g of cotton fabric was immersed therein (bath ratio 50: 1) to graft-pretreat the surface of the cotton fabric, after stirring for 4 hours, 35mL of hybrid luminescent nanoparticles were added thereto and treatment continued at 80 ℃ for 4 hours to graft the hybrid luminescent particles onto the fibers of the cotton fabric. The treated cotton fabric was taken out, washed several times with hot water and then several times with cold water. And taking out the cotton fabric and drying to constant weight to obtain the flexible luminous cotton fabric. After the flexible luminous viscose fabric is respectively cultured with escherichia coli (E.coli) and staphylococcus aureus (S.aureus), the existence of bacteria can be detected through the quenching of the fluorescence signal of the luminous cotton fabric.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (10)
1. A preparation method of a flexible fabric capable of efficiently emitting light to detect bacteria is characterized by comprising the following steps:
(1) preparing a solution: preparing a polyelectrolyte solution by using an acetic acid-sodium acetate buffer solution; preparation of EuCl with ethanol3Solutions, tta solutions and phen solutions;
(2) preparing the hybrid luminescent nano particles: to EuCl3Dripping tta solution into the solution, stirring for 1-2 hours by a magnetic stirrer, adjusting the pH of the reaction solution to 6-8, dripping phen solution into the reaction solution, stirring for 1-5 hours, adding polyelectrolyte solution with negative charges into the reaction solution, and stirring for 1-5 hours to obtain the hybridized luminescent materialNanoparticles;
(3) preparing a flexible luminous fabric: treating the fabric in the reaction solution for 2-10 hours at the temperature of room temperature to 90 ℃, then adding the prepared hybrid luminescent nanoparticles into the reaction solution, keeping the temperature of room temperature to 90 ℃, and treating for 2-10 hours to obtain the flexible fabric; the reaction solution consists of a non-formaldehyde crosslinking agent and a penetrant.
2. The method of claim 1, wherein the EuCl is3The concentration of the ethanol solution is 0.01 mol/L; the concentration of the tta ethanol solution is 0.03 mol/L; the concentration of the phen ethanol solution is 0.01 mol/L.
3. The method of claim 1, wherein the EuCl is3The amount ratio of substances tta and phen EuCl3：tta：phen＝1：3：1。
4. The method according to claim 1, wherein the polyelectrolyte solution is one or two of natural biological polyelectrolytes; the natural biological polyelectrolyte comprises heparin sodium, sodium hyaluronate and chondroitin sulfate.
5. The method of claim 1, wherein the stirring speed is 800rpm to 1500rpm while stirring on the magnetic stirrer.
6. The method of claim 1, wherein the fabric is a polyester cotton cloth, a pure cotton fabric or a viscose fabric; for the pure cotton fabric, before the reaction solution treatment in the step (3), the pure cotton fabric is firstly put into NaOH solution, and then the JFC penetrating agent is dripped and fully mixed; heating the mixed solution to boiling, and keeping boiling for 30 minutes; taking out the treated cotton fabric, washing the cotton fabric for a plurality of times by using hot water, and finally washing the cotton fabric until the cotton fabric is neutral by using cold water; the concentration of the JFC penetrant is 1 g/L; the concentration of the NaOH solution is 10 g/L.
7. The method of claim 1, wherein the non-formaldehyde crosslinking agent is one of 1,2,3, 4-butanetetracarboxylic acid (BTCA), Citric Acid (CA), Pentaerythritol (PER), Maleic Acid (MA), Itaconic Acid (IA); the penetrating agent is one of F-121, T99 and JFC.
8. The method according to claim 1, wherein the bath ratio of the fabric to the reaction solution during the reaction is 1: 50; the concentration of the penetrant during the reaction is 1 g/L.
9. The flexible fabric capable of detecting bacteria by high-efficiency luminescence prepared by the preparation method according to any one of claims 1 to 8.
10. Use of a flexible fabric according to claim 9 for bacterial detection: and (2) respectively culturing the flexible luminous fabric with escherichia coli (E.coli) and staphylococcus aureus (S.aureus) for 12-24 hours at room temperature to 40 ℃, testing the fluorescence spectra of the flexible luminous fabric before and after bacterial culture, and detecting the existence of bacteria through the quenching of the fluorescence signal of the luminous fabric.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010052222.3A CN111270520B (en) | 2020-01-17 | 2020-01-17 | Flexible fabric capable of efficiently emitting light to detect bacteria and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010052222.3A CN111270520B (en) | 2020-01-17 | 2020-01-17 | Flexible fabric capable of efficiently emitting light to detect bacteria and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111270520A CN111270520A (en) | 2020-06-12 |
| CN111270520B true CN111270520B (en) | 2022-03-18 |
Family
ID=71003499
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010052222.3A Active CN111270520B (en) | 2020-01-17 | 2020-01-17 | Flexible fabric capable of efficiently emitting light to detect bacteria and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111270520B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114703658B (en) * | 2022-03-14 | 2024-06-14 | 青岛大学 | Europium-SA nano aggregate crosslinked fluorescence sensing fabric, method and application |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4916060A (en) * | 1985-09-17 | 1990-04-10 | Massachusetts Institute Of Technology | Process for chemical measurement in small volume samples by fluorescent indicators |
| JP2000345037A (en) * | 1999-06-08 | 2000-12-12 | Univ Osaka | Composite material, its preparation, and method for treatment |
| CN1624234A (en) * | 2004-10-22 | 2005-06-08 | 上海工程技术大学 | Modified nanometer dxide multifunction finishing agent, preparation method and its use |
| CN101045200A (en) * | 2007-04-06 | 2007-10-03 | 山东大学 | Method for preparing micro-capsule containing high light-effect rare earth compounding matter |
| CN106350057A (en) * | 2016-08-18 | 2017-01-25 | 青岛大学 | Preparation method of fluorescent hybrid nanoparticles |
| CN108250736A (en) * | 2018-01-24 | 2018-07-06 | 青岛大学 | A kind of rare earth polyelectrolyte compound particle and preparation method thereof |
| CN109776586A (en) * | 2019-02-17 | 2019-05-21 | 青岛大学 | A kind of piece of crystal form be organic-rare-earth complex, luminescent fibre and preparation method thereof |
-
2020
- 2020-01-17 CN CN202010052222.3A patent/CN111270520B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4916060A (en) * | 1985-09-17 | 1990-04-10 | Massachusetts Institute Of Technology | Process for chemical measurement in small volume samples by fluorescent indicators |
| JP2000345037A (en) * | 1999-06-08 | 2000-12-12 | Univ Osaka | Composite material, its preparation, and method for treatment |
| CN1624234A (en) * | 2004-10-22 | 2005-06-08 | 上海工程技术大学 | Modified nanometer dxide multifunction finishing agent, preparation method and its use |
| CN101045200A (en) * | 2007-04-06 | 2007-10-03 | 山东大学 | Method for preparing micro-capsule containing high light-effect rare earth compounding matter |
| CN106350057A (en) * | 2016-08-18 | 2017-01-25 | 青岛大学 | Preparation method of fluorescent hybrid nanoparticles |
| CN108250736A (en) * | 2018-01-24 | 2018-07-06 | 青岛大学 | A kind of rare earth polyelectrolyte compound particle and preparation method thereof |
| CN109776586A (en) * | 2019-02-17 | 2019-05-21 | 青岛大学 | A kind of piece of crystal form be organic-rare-earth complex, luminescent fibre and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 聚电解质粒子的制备和荧光分析;郭俊兰等;《山东工业技术》;20180511(第10期);214 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111270520A (en) | 2020-06-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111270520B (en) | Flexible fabric capable of efficiently emitting light to detect bacteria and preparation method and application thereof | |
| CN108589289B (en) | Method for performing antibacterial finishing on protein fibers based on coupling and enzymatic polymerization | |
| CN109371676B (en) | Polyester fiber product antibacterial processing method based on graft copolymerization | |
| CN109914094A (en) | A kind of preparation method of nano zinc oxide modified aramid fiber | |
| CN101046069A (en) | Method of raising light fastness of natural dye fabric | |
| CN113073464B (en) | Processing method of cellulose fiber product with photothermal effect | |
| CN109680495A (en) | A kind of preparation method and antibacterial fabric of nano particle antibacterial fabric | |
| CN108893990B (en) | Preparation method of multifunctional silk-containing fabric | |
| CN113652879A (en) | Printing and dyeing method for polyester fabric | |
| CN110820368A (en) | Dyeing method for improving dyeing fastness of disperse dye of polyester fabric | |
| CN103923273A (en) | Preparation method of modified feather protein slurry | |
| CN106567241B (en) | A kind of method of alkali decrement treatment chemical fibre Yunlong silk | |
| CN116926944A (en) | Preparation method of titanium-coupled long-acting antibacterial polyester fiber | |
| Li et al. | Biocidal activity of n-halamine methylenebisacrylamide grafted cotton | |
| CN112064381B (en) | Method for dyeing terylene at room temperature | |
| CN109056327A (en) | A method of improving clothes ribbon antibacterial durability | |
| CN114182524A (en) | Antibacterial polyester tatted grey fabric and production process thereof | |
| CN114941239A (en) | Photo-thermal polyester fiber product finishing method based on in-situ deposition | |
| KR20020011806A (en) | Mineral containing dyeing composition and dyeing method by using the same | |
| CN109162090B (en) | A kind for the treatment of process improving Quality Pure Cotton Yarn Production wettability | |
| CN113564922A (en) | Preparation method of fibrilia antibacterial functional material | |
| JP2004292956A (en) | Deodorizing fiber | |
| CN111304937B (en) | Functional compound dyeing acid | |
| JP2001011776A (en) | Electroconductive fiber having increased durability and method for increasing durability of electroconductive fiber | |
| Kodrić et al. | Modeling of disperse dye adsorption on modified polyester fibers |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |