CN111265935A - Concentration method of yeast extract - Google Patents
Concentration method of yeast extract Download PDFInfo
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- CN111265935A CN111265935A CN202010123941.XA CN202010123941A CN111265935A CN 111265935 A CN111265935 A CN 111265935A CN 202010123941 A CN202010123941 A CN 202010123941A CN 111265935 A CN111265935 A CN 111265935A
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- yeast extract
- concentration
- reverse osmosis
- concentrating
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
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- Separation Using Semi-Permeable Membranes (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a yeast extract concentration method, which comprises the steps of freezing a yeast extract solution, filtering at low temperature and high pressure, and finally performing reverse osmosis concentration treatment, wherein the freezing treatment is a combination of the freezing treatment, the low temperature and high pressure filtration and the reverse osmosis concentration, so that redundant water is removed to the maximum extent, the subsequent concentration time is shortened, meanwhile, the low temperature and high pressure filtration is used for further removing redundant water and impurities, the subsequent concentration time is further reduced, and the concentration speed is increased.
Description
Technical Field
The invention relates to the technical field of concentration of an extract, in particular to a method for concentrating a yeast extract.
Background
The fermentation technology is a technology for controlling a fermentation process by using the fermentation action of microorganisms and applying some technical means to produce fermentation products in a large scale. The fermentation technology has the characteristics of high efficiency and specificity, and is widely applied to industrial production at present.
In the process of fermentation production, fermentation conditions are one of the main factors for determining products, another important factor is the post-treatment of fermentation, especially the concentration process of extracts, in the concentration process, heat-sensitive substances in feed liquid are ensured not to be thermally degraded, the production speed is accelerated as much as possible, and the production energy consumption is reduced.
Disclosure of Invention
In view of the above, the present invention is directed to a method for concentrating yeast extract, which overcomes the shortcomings of the prior art methods for concentrating yeast extract.
The invention provides a yeast extract concentration method based on the above purpose, which comprises the following steps:
freezing, namely freezing the yeast extract solution at the temperature of-4-0 ℃ for 30-40 min, and centrifuging to obtain separated ice;
performing low-temperature high-pressure treatment, namely melting and filtering the separated ice at the temperature of 20-30 ℃ and under the pressure of 2-5 Mpa to obtain filtrate;
and (3) reverse osmosis concentration, namely concentrating the filtrate at the temperature of 20-45 ℃ and under the pressure of 4.5-7 Mpa for 1.5-2.5 h to obtain a concentrated solution of the yeast extract.
Optionally, the rotating speed of the centrifugation is 300-500 r/min, and the time duration is 10-30 s.
Optionally, the filtration membrane for the melting filtration treatment is a rolled sodium filtration membrane.
Optionally, the rolled sodium filter membrane is Suntar-372 or Suntar-3M 01.
Optionally, the filtering membrane for reverse osmosis concentration is an RO-2540-46N reverse osmosis concentration membrane.
Optionally, the circulation of the feed liquid for reverse osmosis concentration is 400-500 mL/min.
Optionally, the freezing treatment further comprises adding the positively charged particles into the yeast extract solution, stirring for 10-20 s, and standing and filtering.
From the above, it can be seen that the yeast extract concentration method provided by the invention comprises the steps of freezing the yeast extract solution, filtering at low temperature and high pressure, and performing reverse osmosis concentration, wherein the freezing step is a combination of the freezing step, the low temperature and high pressure filtering step and the reverse osmosis concentration step, so that excessive water is removed to the maximum extent, the subsequent concentration time is shortened, and the low temperature and high pressure filtering step is used for further removing excessive water and impurities, so that the time consumption of the subsequent concentration is further reduced, and the concentration rate is increased.
Detailed Description
In the following description of the embodiments, the detailed description of the present invention, such as the manufacturing processes and the operation and use methods, will be further described in detail to help those skilled in the art to more fully, accurately and deeply understand the inventive concept and technical solutions of the present invention.
In order to solve the problems that in the prior art, a vacuum concentration method is mainly adopted in the concentration method in the yeast production process, the concentration process is slow under the action of vacuum, the energy consumption is high, and the cost is not favorably saved, the concentration method of the yeast extract provided by the invention comprises the following steps:
freezing, namely freezing the yeast extract solution at the temperature of-4-0 ℃ for 30-40 min, and centrifuging to obtain separated ice;
performing low-temperature high-pressure treatment, namely melting and filtering the separated ice at the temperature of 20-30 ℃ and under the pressure of 2-5 Mpa to obtain filtrate;
and (3) reverse osmosis concentration, namely concentrating the filtrate at the temperature of 20-45 ℃ and under the pressure of 4.5-7 Mpa for 1.5-2.5 h to obtain a concentrated solution of the yeast extract.
The yeast extract solution is frozen, then filtered at low temperature and high pressure, and finally subjected to reverse osmosis concentration treatment, through the combination of freezing treatment, low-temperature and high-pressure filtration and reverse osmosis concentration, the freezing treatment removes redundant water to the maximum extent, shortens the subsequent concentration time, and simultaneously filters redundant water and impurities at low temperature and high pressure to further reduce the subsequent concentration time consumption, thereby improving the concentration rate, and on the other hand, the activity of the extract is ensured to the maximum extent due to lower temperature in the treatment process.
Specifically, in order to increase the concentration rate, the rotation speed of the centrifugation is 300-500 r/min, and the time duration is 10-30 s.
Specifically, in order to increase the concentration rate, the filtration membrane for the melt filtration treatment is a rolled sodium filtration membrane.
Specifically, to increase the concentration rate, the rolled sodium filter membrane was Suntar-372 or Suntar-3M 01.
Specifically, in order to improve the concentration rate, the filtering membrane of the reverse osmosis concentration is an RO-2540-46N reverse osmosis concentration membrane.
Specifically, in order to improve the concentration rate, the circulation of the feed liquid for reverse osmosis concentration is 400-500 mL/min.
Specifically, in order to increase the concentration rate, the freezing treatment further comprises adding positive charge particles into the yeast extract solution, stirring for 10-20 s, and standing and filtering.
For example, the method for concentrating yeast extract provided in embodiment 1 of the present invention includes the following steps:
freezing, namely adding 1Kg of yeast extract solution into sulfonated polysulfone particles, stirring for 10-20 s, standing for 20-30 min, filtering, then placing the filtered yeast extract solution into a refrigerator, freezing for 25min at-4 ℃, allowing part of ice crystals to appear in the solution, freezing the organic matters firstly due to the difference between the ice points of the organic matters and water, and allowing the water in the solution to be still liquid, then adding the frozen yeast extract solution into a centrifuge, and centrifuging for 10-30 s at the rotating speed of 300-500 r/min to obtain separated ice, wherein the mass of the separated ice at the moment is 0.5-0.6 Kg.
And (2) performing low-temperature high-pressure treatment, wherein the separated ice is not easy to directly perform reverse osmosis concentration, so that one-step low-temperature high-pressure treatment is added between freezing treatment and reverse osmosis concentration, specifically, the separated ice is subjected to melting filtration treatment at the temperature of 20-30 ℃ and under the pressure of 2-5 Mpa to obtain filtrate, and in order to further concentrate the yeast extract solution, the subsequent reverse osmosis concentration time is saved, the filtration membrane in the low-temperature high-pressure treatment is a Suntar-372 roll sodium filtration membrane, and 0.45-0.55 kg of yeast extract filtrate is obtained.
And (2) reverse osmosis concentration, namely putting the yeast extract filtrate into reverse osmosis concentration equipment, wherein a filtering membrane used by the reverse osmosis concentration equipment is an RO-2540-46N reverse osmosis concentration membrane, circulating the yeast extract filtrate in a closed circuit for concentration, and simultaneously generating heat in the circulating process of the yeast extract filtrate, so that the damage of overhigh temperature to the activity of active ingredients in the extract is prevented, the reverse osmosis concentration is carried out at the temperature of 20-45 ℃, the concentration is carried out at the pressure of 4.5-7 MPa for improving the concentration rate, the circulation of the reverse osmosis concentration is 400-500 mL/min for ensuring the maximum maintenance of the active ingredients in the yeast extract, and the circulation time is 1.5-2.5 h, so that the concentrated solution of the yeast extract is obtained.
For example, the method for concentrating a yeast extract provided in embodiment 2 of the present invention is similar to embodiment 1 of the present invention, except that in the reverse osmosis concentration, the temperature of the reverse osmosis concentration is 20 ℃, the concentration is performed under the pressure of 4.5Mpa in order to increase the concentration rate, and in order to ensure the maximum retention of the effective components in the yeast extract, the flow rate of the feed liquid of the reverse osmosis concentration is 400mL/min, and the cycle time is 1.5h, so as to obtain the concentrated solution of the yeast extract.
For example, the difference between the method for concentrating yeast extract provided in embodiment 3 of the present invention and embodiment 1 of the present invention is that, in the reverse osmosis concentration, the temperature of the reverse osmosis concentration is 30 ℃, the concentration is performed under the pressure of 5.5Mpa in order to increase the concentration rate, and in order to ensure the maximum retention of the effective components in the yeast extract, the throughput of the feed liquid of the reverse osmosis concentration is 450mL/min, and the cycle time is 2 hours, so as to obtain the concentrated solution of yeast extract.
For example, the embodiment 4 of the present invention provides a method for concentrating a yeast extract, which is similar to the embodiment 1 of the present invention, except that in the reverse osmosis concentration, the temperature of the reverse osmosis concentration is 40 ℃, the concentration is performed under the pressure of 6.5Mpa in order to increase the concentration rate, and in order to ensure the maximum retention of the effective components in the yeast extract, the flow rate of the feed liquid of the reverse osmosis concentration is 450mL/min, and the cycle time is 2h, so as to obtain the concentrated solution of the yeast extract.
For example, the difference between the method for concentrating yeast extract provided in embodiment 5 of the present invention and embodiment 1 of the present invention is that, in the reverse osmosis concentration, the temperature of the reverse osmosis concentration is 45 ℃, the concentration is performed under the pressure of 7Mpa in order to increase the concentration rate, and in order to ensure the maximum retention of the effective components in the yeast extract, the throughput of the feed liquid of the reverse osmosis concentration is 450mL/min, and the cycle time is 2h, so as to obtain the concentrated solution of yeast extract.
For example, the method for concentrating a yeast extract provided in embodiment 6 of the present invention is similar to embodiment 1 of the present invention, except that in the reverse osmosis concentration, the temperature of the reverse osmosis concentration is 40 ℃, the concentration is performed under the pressure of 6.5Mpa in order to increase the concentration rate, and in order to ensure the maximum retention of the effective components in the yeast extract, the flow rate of the feed liquid of the reverse osmosis concentration is 500mL/min, and the cycle time is 2.5h, so as to obtain the concentrated solution of the yeast extract.
For example, the method for concentrating yeast extract according to example 7 of the present invention is similar to example 1 of the present invention, except that the vacuum concentration method described in the literature (Liu mist Sha, Wu Qiaoshi, Hu Shi Gao, etc.. the effect of different concentration methods on the quality of fermented mango jam [ J ]. food technology, 2019,44(09):99-102.) is used.
The trehalose content of a yeast extract is one of its main components and can be used to characterize the quality of a yeast extract, and is therefore characterized by the following measurement methods:
(1) measuring trehalose by an optical rotation method;
(2) and (3) calculating yield: the trehalose permeability is based on the concentration of solute in the concentrated solution, and the calculation formula is as follows:
the measurement results are shown in the following table:
the measurement result shows that the yield of the trehalose obtained by the method provided by the embodiment of the invention is far higher than that obtained by the existing method at the same concentration temperature.
Those of ordinary skill in the art will understand that: the discussion of any embodiment above is meant to be exemplary only, and is not intended to intimate that the scope of the disclosure, including the claims, is limited to these examples; within the idea of the invention, also features in the above embodiments or in different embodiments may be combined, steps may be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.
The embodiments of the invention are intended to embrace all such alternatives, modifications and variances that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, substitutions, improvements and the like that may be made without departing from the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (7)
1. A method for concentrating a yeast extract, comprising the steps of:
freezing, namely freezing the yeast extract solution at the temperature of-4-0 ℃ for 30-40 min, and centrifuging to obtain separated ice;
performing low-temperature high-pressure treatment, namely melting and filtering the separated ice at the temperature of 20-30 ℃ and under the pressure of 2-5 Mpa to obtain filtrate;
and (3) reverse osmosis concentration, namely concentrating the filtrate at the temperature of 20-45 ℃ and under the pressure of 4.5-7 Mpa for 1.5-2.5 h to obtain a concentrated solution of the yeast extract.
2. The method for concentrating yeast extract according to claim 1, wherein the rotation speed of the centrifugation is 300 to 500r/min and the time period is 10 to 30 s.
3. The method for concentrating a yeast extract according to claim 1, wherein the filtration membrane for the melt filtration treatment is a rolled sodium filtration membrane.
4. The method for concentrating yeast extract according to claim 3, wherein the rolled nanofiltration membrane is Suntar-372 or Suntar-3M 01.
5. The method for concentrating yeast extract according to claim 1, wherein the reverse osmosis concentrated filtration membrane is a RO-2540-46N reverse osmosis concentrated membrane.
6. The method for concentrating a yeast extract according to claim 1, wherein a flow rate of the feed liquid for the reverse osmosis concentration is 400 to 500 mL/min.
7. The method for concentrating yeast extract according to claim 1, wherein the freezing treatment further comprises adding positively charged particles to the yeast extract solution, stirring for 10 to 20 seconds, and then standing and filtering.
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| CN202010123941.XA CN111265935A (en) | 2020-02-27 | 2020-02-27 | Concentration method of yeast extract |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3540513A (en) * | 1968-10-14 | 1970-11-17 | Daido Chem Eng Corp | Apparatus for treating a pickling waste |
| CN101366727A (en) * | 2008-09-27 | 2009-02-18 | 高翔 | Method for preparing calf blood protein-removed extract and products thereby |
| CN101898941A (en) * | 2010-07-27 | 2010-12-01 | 中山大学 | Method for purifying trans-anethole |
| CN102423328A (en) * | 2011-09-15 | 2012-04-25 | 北京建生药业有限公司 | Traditional Chinese medicine composition for treating cerebral apoplexy and its preparation method |
| CN105682762A (en) * | 2013-08-29 | 2016-06-15 | 株式会社明治 | Production method for concentrated product using membrane-concentration method and freeze-concentration method |
| CN110117019A (en) * | 2019-05-27 | 2019-08-13 | 四川思达能环保科技有限公司 | Lithium hydroxide production technology |
-
2020
- 2020-02-27 CN CN202010123941.XA patent/CN111265935A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3540513A (en) * | 1968-10-14 | 1970-11-17 | Daido Chem Eng Corp | Apparatus for treating a pickling waste |
| CN101366727A (en) * | 2008-09-27 | 2009-02-18 | 高翔 | Method for preparing calf blood protein-removed extract and products thereby |
| CN101898941A (en) * | 2010-07-27 | 2010-12-01 | 中山大学 | Method for purifying trans-anethole |
| CN102423328A (en) * | 2011-09-15 | 2012-04-25 | 北京建生药业有限公司 | Traditional Chinese medicine composition for treating cerebral apoplexy and its preparation method |
| CN105682762A (en) * | 2013-08-29 | 2016-06-15 | 株式会社明治 | Production method for concentrated product using membrane-concentration method and freeze-concentration method |
| CN110117019A (en) * | 2019-05-27 | 2019-08-13 | 四川思达能环保科技有限公司 | Lithium hydroxide production technology |
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Application publication date: 20200612 |