CN111257948A - Sensor matrix-based sample detection plate and sample detection method - Google Patents
Sensor matrix-based sample detection plate and sample detection method Download PDFInfo
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- CN111257948A CN111257948A CN202010080639.0A CN202010080639A CN111257948A CN 111257948 A CN111257948 A CN 111257948A CN 202010080639 A CN202010080639 A CN 202010080639A CN 111257948 A CN111257948 A CN 111257948A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01V—GEOPHYSICS; GRAVITATIONAL MEASUREMENTS; DETECTING MASSES OR OBJECTS; TAGS
- G01V3/00—Electric or magnetic prospecting or detecting; Measuring magnetic field characteristics of the earth, e.g. declination, deviation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01V—GEOPHYSICS; GRAVITATIONAL MEASUREMENTS; DETECTING MASSES OR OBJECTS; TAGS
- G01V3/00—Electric or magnetic prospecting or detecting; Measuring magnetic field characteristics of the earth, e.g. declination, deviation
- G01V3/08—Electric or magnetic prospecting or detecting; Measuring magnetic field characteristics of the earth, e.g. declination, deviation operating with magnetic or electric fields produced or modified by objects or geological structures or by detecting devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01V—GEOPHYSICS; GRAVITATIONAL MEASUREMENTS; DETECTING MASSES OR OBJECTS; TAGS
- G01V8/00—Prospecting or detecting by optical means
- G01V8/10—Detecting, e.g. by using light barriers
Abstract
The application discloses a sensor matrix-based sample detection plate and a sample detection method. Wherein the sensor matrix based sample detection plate is applied to a sample holder having a plurality of well sites for insertion of sample containers, the sensor matrix based sample detection plate being disposed at the bottom of the sample holder; the sensor matrix-based sample detection plate includes: and the sensors are arranged on the surface of the sample detection plate based on the sensor matrix, are aligned with the hole sites and are used for detecting whether the sample containers are inserted into the hole sites or not. By the method and the device, timely feedback of the sample taking state is achieved, and the problem that the sample taking state cannot be fed back timely by a bar code sample management system adopted in the related technology is solved.
Description
Technical Field
The application relates to the field of experimental instruments and equipment, in particular to a sensor matrix-based sample detection plate and a sample detection method.
Background
The representativeness, the effectiveness and the integrity of a laboratory sample directly influence the accuracy of a test result, and the management of the sample ensures that the quality of the sample is not changed in the whole detection process and the sample is not damaged, lost, confused or deteriorated in the storage period.
In order to realize management of a sample taking state, a barcode sample management system and a management method thereof are provided in the related art. The system and the management method comprise the following steps: the system is characterized by comprising a bar code, a system computer, a bar code printer, a bar code scanning gun and a mobile bar code scanning gun, wherein the system computer is respectively connected with the bar code printer and the bar code scanning gun. By adopting the system, the bar code sample management is adopted in a product detection station or a laboratory, and the combination of software and hardware expands the sample information, improves the working efficiency, quickly queries the sample information and is beneficial to scientific and efficient management of the sample.
However, research finds that the system provided by the related art needs to be equipped with a code scanning gun, a barcode printer and other equipment, so that not only is the construction cost high, but also the sample barcode needs to be frequently scanned for sample taking and registering in sample management, and the sample taking and registering process is complicated. Moreover, the system provided by the related art only performs planning and virtual management on the system, cannot be directly linked with a real object, and cannot timely feed back the situations of more fetching, less fetching, wrong fetching and the like in the actual operation process of a user, so that the subsequent remedial adjustment measures are complicated.
Disclosure of Invention
Based on this, it is necessary to provide a sample detection plate based on a sensor matrix and a sample detection method, at least for the problem that the barcode sample management system adopted in the related art cannot timely feed back the sample taking state.
In a first aspect, an embodiment of the present application provides a sensor matrix-based sample detection plate for use in a sample rack, the sample rack having a plurality of holes for sample containers to be inserted into, the sensor matrix-based sample detection plate being disposed at a bottom of the sample rack, the sensor matrix-based sample detection plate comprising: and the sensors are arranged on the surface of the sample detection plate based on the sensor matrix, are aligned with the hole sites and are used for detecting whether the sample containers are inserted into the hole sites or not.
In some embodiments, the number of the sensors is multiple, the sensors are arranged in a matrix manner, and the sensors are aligned with the hole sites of the sample holder one by one.
In some of these embodiments, the sensor comprises: a proximity sensor or a contact sensor; wherein the proximity sensor comprises one of: the system comprises a radio frequency identification reader, a photoelectric proximity sensor, a magnetoelectric sensor, a capacitive sensor and an inductive sensor; the contact sensor includes: pressure type resistance switch.
In some of these embodiments, the sensor matrix-based sample detection plate further comprises: the detection processing unit comprises a power supply end and a detection end, wherein the input end of the sensor is electrically connected to the power supply end, the output end of the sensor is electrically connected to the grounding end, and the detection end is electrically connected to the input end of the sensor; the sensor is used for switching on or off according to whether the sample container inserted into the hole site is detected; the detection processing unit is used for supplying power to the sensor through the power supply end and detecting the level state of the input end of the sensor through the detection end.
In some embodiments, the number of the sensors is multiple, and the multiple sensors are arranged in a matrix manner, wherein the input ends of the sensors with the same row number are connected in parallel to the power supply end corresponding to the same row number; and the input ends of the sensors with the same column number are electrically connected with the detection ends corresponding to the same column number.
In some of these embodiments, the sensor is a reflective optoelectronic coupler comprising a light emitting section and a photoelectric conversion section; wherein an input terminal of the light emitting section and an input terminal of the photoelectric conversion section are electrically connected and serve as an input terminal of the reflective photoelectric coupler; the output terminal of the light emitting section and the output terminal of the photoelectric conversion section are electrically connected and serve as the output terminal of the reflective photoelectric coupler.
In some of these embodiments, the sensor matrix-based sample detection plate further comprises: the detection trigger unit is electrically connected with the detection processing unit and is used for detecting whether the sample rack is used or not and generating a detection trigger signal to the detection processing unit after the sample rack is used, wherein the detection trigger signal is used for indicating the detection processing unit to acquire the level state of the input end of each sensor in the plurality of sensors.
In a second aspect, an embodiment of the present application provides a sample detection method applied to the sensor matrix-based sample detection plate of the first aspect, where the sensor matrix-based sample detection plate is placed at the bottom of the sample rack, and the method includes: after the sensor is powered on, acquiring a detection result of the sensor; and determining the taking state of the sample on the sample rack according to the detection result.
In some embodiments, before obtaining the detection result of the sensor, the method further includes: receiving a detection trigger signal; and powering on the sensor according to the detection trigger signal.
In some embodiments, after the sensor is powered on, acquiring the detection result of the sensor includes: powering on a row of sensors with the same row number in the plurality of sensors through the power supply terminal; detecting the level state of the input end of the sensor with different column numbers in the row of sensors through the detection end, and taking the level state as the detection result of the sensor corresponding to the row-column intersection point; wherein a high level in the level state indicates that no sample container is inserted in the well site corresponding to the sensor, and a low level in the level state indicates that a sample container is inserted in the well site corresponding to the sensor.
Compared with the prior art, through the sensor matrix-based sample detection plate and the sample detection method provided by the embodiment of the application, the sensor matrix-based sample detection plate applied to the sample rack is adopted, the sample rack is provided with a plurality of hole sites for inserting sample containers, the sensor matrix-based sample detection plate is arranged at the bottom of the sample rack, and the sensor matrix-based sample detection plate comprises: and the sensors are arranged on the surface of the sample detection plate based on the sensor matrix, are aligned with the hole sites and are used for detecting whether the sample containers are inserted into the hole sites or not. The embodiment of the application realizes timely feedback of the sample taking state through the mode, and solves the problem that a bar code sample management system adopted in the related technology cannot timely feed back the sample taking state.
Drawings
In order to more clearly illustrate the embodiments of the present application or technical solutions in related arts, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic view of a structure of a sample holder according to the related art;
FIG. 2 is a schematic diagram of a structure of a sensor matrix-based sample detection plate according to an embodiment of the present application;
FIG. 3 is a block diagram of a circuit configuration for a sensor matrix based sample detection plate according to an embodiment of the present application;
FIG. 4 is a circuit topology diagram of one sensor in a sensor matrix based sample detection plate according to an embodiment of the present application;
FIG. 5 is a preferred circuit topology for a sensor matrix based sample detection plate according to an embodiment of the present application;
FIG. 6 is a preferred flow chart of a method of sample detection according to an embodiment of the present application;
fig. 7 is a flow chart of a sample detection method according to an embodiment of the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
It is obvious that the drawings in the following description are only examples or embodiments of the application, from which the application can also be applied to other similar scenarios without inventive effort for a person skilled in the art. Moreover, it should be appreciated that in the development of any such actual implementation, as in any engineering or design project, numerous implementation-specific decisions must be made to achieve the developers' specific goals, such as compliance with system-related and business-related constraints, which may vary from one implementation to another.
Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by those of ordinary skill in the art to which this application belongs. The use of "first," "second," and similar terms in the description and claims of this patent application do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The terms "a," "an," "the," and the like, do not denote a limitation of quantity, and may denote the singular or plural.
The word "comprise" or "comprises", and the like, means that the element or item listed before "comprises" or "comprising" covers the element or item listed after "comprising" or "comprises" and its equivalent, and does not exclude other elements or items. "connected" or "coupled" and similar terms are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect.
Typically, samples are stored in sample containers, such as sample tubes, sample plastic bottles, and the sample containers are held using corresponding sample holders. The sample holders typically have a plurality of wells into which sample containers are inserted for retention. The sample holders are typically placed in a sample cabinet for long periods of storage.
FIG. 1 is a schematic view of a sample holder according to the related art, and as shown in FIG. 1, the sample holder generally has a plurality of well sites arranged in a matrix manner, i.e., in rows and columns. The size of the hole site is determined according to the size of the outer diameter of the sample container to be inserted. The sample holder has at least one layer of partition (the partition is usually horizontally arranged and can be inclined to the horizontal plane), and in order to avoid the inclination of the sample container, the sample holder usually has at least two layers of partitions. The sample holder shown in FIG. 1 has three layers of partitions, wherein the lowermost partition may or may not be perforated.
In this embodiment, a sensor matrix based sample detection plate is provided for application to a sample holder. The sample holder has at least a plurality of well sites for insertion of sample containers, and a sample detection plate based on a sensor matrix is disposed at the bottom of the sample holder.
In this embodiment, the bottom of the sample holder refers to a position of the bottom where the bottom of the sample container can contact or approach after the sample container is inserted into the hole of the sample holder. For example, in the sample holder with three layers of partition boards shown in FIG. 1, in the case that the lowest layer of partition board is provided with through holes, after the sample container is inserted into the hole site, the bottom of the sample container is directly contacted with or close to the plane on which the sample holder is placed, and then the bottom of the sample holder in this embodiment refers to the position between the plane and the bottom surface of the lowest layer of partition board of the sample holder. In the case that the lowermost partition plate is not provided with a through hole, after the sample container is inserted into the hole, the bottom of the sample container directly contacts or is close to the surface of the lowermost partition plate, and then the bottom of the sample rack in this embodiment refers to the position from the top surface of the lowermost partition plate to the bottom surface of the intermediate partition plate.
Fig. 2 is a schematic structural view of a sensor matrix-based sample detection plate according to an embodiment of the present application, and as shown in fig. 2, a sensor matrix-based sample detection plate 10 includes: and a sensor 20, wherein the sensor 20 is disposed on the surface of the sample detection plate 10 based on the sensor matrix, and when the sample detection plate 10 based on the sensor matrix is used, the sample detection plate 10 based on the sensor matrix is aligned with the well sites of the sample rack for detecting whether the sample containers are inserted into the well sites of the sample rack.
By adopting the sample detection plate based on the sensor matrix, when the sample container is inserted into the detection hole site or the sample container is taken out from the detection hole site, the sensor 20 can detect whether the sample container is inserted into the detection hole site, thereby detecting the sample taking state of the corresponding hole site on the sample rack.
Compared with the mode of adopting bar codes and code scanning guns in the related art, when the sample detection plate based on the sensor matrix is adopted to detect the sample taking state, code scanning operation is not required, and convenience is provided for simplifying the taking and registering of the sample. Adopt above-mentioned sample detection board based on sensor matrix to detect the sample and take the state, no longer need print and post the bar code for every sample container, reduced the cost of labor. The sample detection plate based on the sensor matrix is arranged at the bottom of the sample frame in the using process, and does not occupy extra space. In addition, through the sample detection plate based on the sensor matrix, the sample management system can be connected with a real object (namely a sample container), so that the real object can be monitored, and compared with a bar code and code scanning gun mode adopted in the related technology, the conditions of more samples, less samples, wrong samples and the like in the actual operation process of a user can be found in time.
In some embodiments, at least one sensor may be correspondingly disposed at the bottom of each well site of each sample rack, or at least one sensor may be correspondingly disposed at the bottom of each well site that is interested by one or more users, for example, the well site that is interested by one or more users may be a well site for placing a valuable sample.
When the hole sites of the sample holder are arranged in a matrix manner, a plurality of sensors arranged on the sample detection plate based on the sensor matrix are also arranged in a matrix manner, and the plurality of sensors arranged on the sample detection plate based on the sensor matrix are aligned with the hole sites of the sample holder one by one. It should be noted that the hole sites of the sample holder may be arranged in other manners, and the plurality of sensors disposed on the sample detection plate based on the sensor matrix are arranged in the same manner and at the same arrangement distance as the hole sites of the sample holder, so that the plurality of sensors disposed on the sample detection plate based on the sensor matrix are aligned with the hole sites of the sample holder one by one. Through the mode, whether the sample container is inserted into each hole position of the sample rack or not can be detected.
The sensor employed in the present embodiment may be any sensor capable of enabling detection of the presence of an object, including but not limited to a proximity sensor or a contact sensor. Wherein, proximity sensor refers to a non-contact sensor, for example including but not limited to one of the following: the system comprises a radio frequency identification reader, a photoelectric proximity sensor, a magnetoelectric sensor, a capacitive sensor and an inductive sensor; contact sensors include, but are not limited to: pressure type resistance switch.
Among these, pressure-based resistive switches, capacitive sensors, and photoelectric proximity sensors typically allow their detection function without the need for special identification of the sample container. For example, when a pressure-type resistance switch is used as the sensor, the bottom of the sample container inserted into the well is pressed against the pressure-type resistance switch, and the pressure-type resistance switch outputs an electrical signal indicating that the sample container is inserted into the well; when the sample container is taken out from the hole site, the bottom of the sample container is separated from the pressure type resistance switch, and the pressure type resistance switch outputs another electric signal to indicate that the sample container in the hole site is taken out.
Among them, the rfid reader and the magnetoelectric sensor generally need to perform special identification on the sample container. For example, when a magnetoelectric sensor is used as the sensor, a magnetic medium label is usually attached to the bottom of the sample container; when an rfid reader is used as the sensor, an rfid tag is usually attached to the bottom of the sample container. However, the above-mentioned sensors using the rfid reader and the magnetoelectric sensor have an advantage in that information related to the sample stored in the sample container can be written in the magnetic medium tag or the rfid tag, so that whether a corresponding sample is returned or not and whether a correct sample is taken or not can be confirmed, thereby achieving more detailed management of the sample taking state.
The sensors in some preferred embodiments of the present application employ photoelectric proximity sensors, preferably reflective photoelectric couplers. The basic structure of a reflective photo-electric coupler comprises an infrared emitting tube and a receiving tube, wherein the receiving tube is usually made of a photosensitive material. In some embodiments, the infrared transmitting tube of the reflective photoelectric coupler emits infrared light, the infrared light is blocked by the detected object and reflected in a short distance, and the receiving tube determines whether the detected object exists above the receiving tube according to the intensity of the reflected infrared light. In the present embodiment, a 5V power supply unit is used, and the reflective photoelectric coupler used has an output voltage of 0.3V to 0.6V when an object is detected and an output voltage of 4.5V or more when an object is not detected. Since 0.3V to 0.6V can be identified as a low level signal (i.e. signal 0) by most of the conversion chips or singlechips, and 4.5V can be identified as a high level signal (i.e. signal 1) by most of the conversion chips or singlechips, whether an object exists above the reflective photoelectric coupler can be converted into a switching signal of 0 or 1, and the switching signal is transmitted to other conversion chips or singlechips for digital identification.
According to the above-described operation principle of the reflective type photoelectric coupler, in most cases, even if any reflective material is not provided at the bottom of the sample container, the reflection of light by the sample container material itself can make the receiving tube conductive. In order to improve the response sensitivity of the reflective photoelectric coupler, a reflective sticker or coating made of a material with excellent reflection performance can be adhered or coated on the bottom of the sample container, so that the reflectivity of the bottom of the sample container is increased, the intensity of infrared light reflected to a receiving tube of the reflective photoelectric coupler is improved, and the possibility of detection errors of the reflective photoelectric coupler is reduced.
In some embodiments, the reflective optical coupler is a reflective optical coupler packaged in a patch form, and can be conveniently manufactured. The reflective optical-electrical coupler packaged in the patch form can be a patch switch with the model number ITR20001, for example. The patch switch has the advantages of quick response time, high sensitivity, light, thin and compact product and capability of shielding the influence of visible wavelength.
Fig. 3 is a block diagram of a circuit structure of a sensor matrix-based sample detection plate according to an embodiment of the present disclosure, as shown in fig. 3, in some embodiments, the sensor matrix-based sample detection plate may further include a detection processing unit 30, where the detection processing unit 30 includes a power supply terminal 31 and a detection terminal 32, where an input terminal of the sensor 20 is electrically connected to the power supply terminal 31, an output terminal of the sensor 20 is electrically connected to a ground terminal GND, and the detection terminal 32 is electrically connected to an input terminal of the sensor 20; the sensor 20 is used for switching on or off according to whether the sample container inserted into the hole site is detected; the detection processing unit 30 is used for supplying power to the sensor 20 through the power supply terminal 31 and detecting the level state of the input terminal of the sensor 20 through the detection terminal 32.
In the circuit configuration shown in fig. 3, when the power supply terminal 31 of the detection processing unit 30 outputs a high level to supply power to the sensor 20, and the sensor 20 is turned on after detecting that a sample container is inserted into a hole, the level of the detection terminal 32 of the detection processing unit 30 is pulled down to a low level; when the power supply terminal 31 of the detection processing unit 30 outputs a high level to supply power to the sensor 20, and the sensor 20 is disconnected when detecting that no sample container is inserted into the hole, the level detected by the detection terminal 32 of the detection processing unit 30 is equal to the level of the power supply terminal 31, i.e. the high level. Therefore, in the circuit configuration shown in fig. 3, the detection of a low level at the detection terminal 32 indicates that a sample container is inserted into the corresponding well; the detection of high level at the detection end 32 indicates that no sample container is inserted into the corresponding hole site, thereby realizing the detection of the sample taking state in the corresponding hole site.
Fig. 4 is a circuit topology diagram of one sensor in the sample detection plate based on the sensor matrix according to the embodiment of the present application, and as shown in fig. 4, a description will be given by taking as an example that the sensor for detecting the state of sample taking is a reflective type photoelectric coupler. The reflective photoelectric coupler has four pins, namely an input terminal 1 and an output terminal 2 of the light emitting part (i.e. infrared emission tube), and an input terminal 4 and an output terminal 3 of the photoelectric conversion part (i.e. receiving tube). The input terminal 1 of the light emitting section and the input terminal 4 of the photoelectric conversion section are electrically connected to the power supply terminal 31, the output terminal 2 of the light emitting section and the output terminal 3 of the photoelectric conversion section are electrically connected to the ground terminal GND, and the detection terminal 32 is electrically connected to the input terminal 4 of the photoelectric conversion section. In fig. 4, the resistor R1 and the resistor R2 function as a current limiter to prevent the light emitting part or the photoelectric conversion part from being burned out due to excessive current.
The sample detection plate based on the sensor matrix can detect the taking state of samples in a plurality of hole sites, and the maximum number of the hole sites which can be detected depends on the number of the sensors correspondingly arranged on the sample detection plate based on the sensor matrix. In some sample holders, the number of wells may be large, for example, 10 × 10 wells, and if the sample access status in each well needs to be detected, 100 sensors are required. It is possible to provide 100 sensors on the sample detection plate based on the sensor matrix, however, if each sensor individually occupies one power supply terminal and one detection terminal of the detection processing unit, at least 200 pins are required to meet the requirement, and it is obvious that when the number of sensors on the sample detection plate based on the sensor matrix is large, the problem of difficulty in type selection of the detection processing unit may be faced.
In order to solve the problem that the detection processing unit is difficult to select due to the fact that the number of sensors on the sample detection plate based on the sensor matrix is large and a large number of pins are occupied, in some embodiments, when the number of the sensors arranged on the sample detection plate based on the sensor matrix is multiple and the multiple sensors are arranged in a matrix-like mode, a power supply end is multiplexed for each row of the sensors, and a detection end is multiplexed for each column of the sensors, so that the input ends of the sensors with the same row number can be connected in parallel to the power supply end corresponding to the same row number, and the input ends of the sensors with the same column number are electrically connected with the detection end corresponding to the same column number.
Fig. 5 is a preferred circuit topology diagram of the sample detection plate based on the sensor matrix according to the embodiment of the present application, and as shown in fig. 5, the description is still given by taking as an example that the sensor as the state for detecting the sample access is a reflective type photoelectric coupler, in which the input terminal of the light emitting portion and the input terminal of the photoelectric conversion portion of the reflective type photoelectric coupler of the same row number are electrically connected to the power supply terminal corresponding to the same row number, respectively, and the input terminal of the photoelectric conversion portion of the reflective type photoelectric coupler of the same column number is electrically connected to the detection terminal corresponding to the same column number. Further, the output terminals of the light emitting portions and the output terminals of the photoelectric conversion portions of all the reflective type photoelectric couplers are electrically connected to the ground GND (not shown in fig. 5). Peripheral circuits of the detection processing unit, such as a power supply circuit and the like, are also omitted in fig. 5.
With continued reference to fig. 5, 9 reflective photo-couplers U70 to U78 arranged in a 3 × 3 matrix form, and a single chip microcomputer U79 as a detection processing unit are exemplarily shown in fig. 5. Pins P0.0, P0.1 and P0.2 of the singlechip U79 are power supply terminals and are used for providing row information; the P2.5, P2.6 and P2.7 pins are the detection terminals for receiving column information.
Fig. 6 is a preferred flowchart of a sample detection method according to an embodiment of the present application, and as shown in fig. 6, with the sample detection board based on the sensor matrix having the circuit topology shown in fig. 5, each time the single-chip microcomputer U79 obtains a detection result of the reflective photoelectric coupler, the single-chip microcomputer may send all 1 signals (i.e., high levels) to the port P0.0, obtain TTL high levels on the line, and record the line number X as 1. Then, whether or not a low level appears on P2.5, P2.6, and P2.7 is read, and if so, the column number is recorded. For example, when P2.6 detects a low level, the column number Y is recorded as 2, and the position information XY is finally recorded or outputted as 12, that is, the hole corresponding to the position information XY 12 is indicated, that is, the sample container is inserted into the second hole in the first row. Subsequently, P0.0 is turned off, P1.0 is turned on, the row number X of the next row is recorded as 2, and the detection of the second row is started. And by analogy, finally completing the collection of the detection result of each photoelectric coupler matrix in the 3 multiplied by 3 reflection type photoelectric coupler matrix, and determining the taking state of the sample container of each hole site corresponding to each position.
Still take a sample detection board based on a sensor matrix of 10 × 10 sensors as an example, a detection processing unit of 200 pins is originally needed; after the power supply end and the detection end are multiplexed according to rows and columns in the embodiment, the acquisition requirements of detection results of 10 multiplied by 10 sensors can be met only by 20 pins, so that the occupation of the pins of the detection processing unit is greatly reduced, and the difficulty in type selection of the detection processing unit is reduced.
In some embodiments, the single chip microcomputer can also transmit all the finally collected detection results to the upper computer for comparison, so as to confirm whether the state of the taken sample is consistent with the preset state. Through the mode, the beneficial effect of monitoring the real-time state of the sample is also achieved. And the singlechip is in direct communication with the upper computer, so that communication delay is reduced, and the response of the upper computer is quicker.
In some embodiments, the upper computer may further send an alarm to remind an administrator of manual processing when determining that the actual taking state of the sample is inconsistent with the preset state.
In some embodiments, the detection processing unit is powered by 5V, and the high level output by the power supply terminal is also 4.5V to 5V.
In some embodiments, the detection processing unit may periodically initiate the collection of the detection result of each sensor, or may trigger the collection of the detection result by a specific event. For example, a sensor matrix-based sample detection plate may further comprise: and the detection trigger unit is electrically connected with the detection processing unit and is used for detecting whether the sample rack is used or not and generating a detection trigger signal to the detection processing unit after the sample rack is used, wherein the detection trigger signal is used for indicating the detection processing unit to acquire the detection result of each sensor in the plurality of sensors.
The detection trigger unit may be, for example, a photoelectric sensor, which may be disposed in the sample drawer cabinet, and the photoelectric sensor determines that the sample rack is used by detecting a change in brightness of primary light each time the sample drawer cabinet is opened and closed, after which the photoelectric sensor generates a detection trigger signal to the detection processing unit to instruct the detection processing unit to acquire a detection result of each sensor.
It should be noted that the detection triggering unit may also be another sensor capable of achieving the same purpose, for example, a pressure sensor disposed under the base of the sample holder detects a change in pressure of the sample holder on the table top to determine whether the sample holder is used, or a contact sensor disposed on the cabinet door of the sample drawer cabinet detects an open/close state of the cabinet door of the sample drawer cabinet to determine whether the sample holder is used.
The embodiment also provides a sample detection method, which is applied to the sensor matrix-based sample detection plate provided by the embodiment, and the sensor matrix-based sample detection plate is placed at the bottom of the sample rack when in use. Fig. 7 is a flowchart of a sample detection method according to an embodiment of the present application, and as shown in fig. 7, the flowchart includes the following steps:
step S701, after the sensor is electrified, obtaining a detection result of the sensor;
step S702, determining the taking state of the sample on the sample rack according to the detection result.
In some embodiments, before step S701, the method further comprises: receiving a detection trigger signal; and powering on the sensor according to the detection trigger signal. The detection trigger signal may be generated at regular time or according to the use state of the sample holder.
In some embodiments, step S701 includes: electrifying a row of sensors with the same row number in the plurality of sensors through a power supply terminal; detecting the level state of the input end of the sensor with different column numbers in the row of sensors through the detection end, and taking the level state as the detection result of the sensor corresponding to the row-column intersection point; wherein a high level in the level state indicates that no sample container is inserted in the well site corresponding to the sensor, and a low level in the level state indicates that a sample container is inserted in the well site corresponding to the sensor.
In summary, through the above-mentioned embodiment or the implementation mode that this application provided, through place the sensor in the below of sample frame to with host computer real-time communication, the monitoring sample condition of taking can be accurate to the taking out and returning of each sample, thereby stop strictly that the sample is by mistake taken, take the waste that causes more. The sensor is arranged in a matrix form in the application, so that the sample taking state of a plurality of hole sites is detected, the circuit is simple in structure and low in cost, the convenience of detecting the sample taking state is greatly improved, and the cost is reduced. In the embodiment of the application, the pins of the detection processing unit are multiplexed according to rows and columns, so that the type selection difficulty of the detection processing unit is reduced.
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A sensor matrix-based sample detection plate for use in a sample holder having a plurality of wells for insertion of sample containers, wherein said sensor matrix-based sample detection plate is disposed at the bottom of the sample holder, said sensor matrix-based sample detection plate comprising: and the sensors are arranged on the surface of the sample detection plate based on the sensor matrix, are aligned with the hole sites and are used for detecting whether the sample containers are inserted into the hole sites or not.
2. The sensor matrix-based sample detection plate of claim 1, wherein the number of sensors is plural, the plurality of sensors are arranged in a matrix manner, and the plurality of sensors are aligned with the well sites of the sample holder one by one.
3. A sensor matrix-based sample detection plate according to claim 1, wherein said sensors comprise: a proximity sensor or a contact sensor; wherein the proximity sensor comprises one of: the system comprises a radio frequency identification reader, a photoelectric proximity sensor, a magnetoelectric sensor, a capacitive sensor and an inductive sensor; the contact sensor includes: pressure type resistance switch.
4. The sensor matrix-based sample detection plate of claim 1, wherein said sensor matrix-based sample detection plate further comprises: the detection processing unit comprises a power supply end and a detection end, wherein the input end of the sensor is electrically connected to the power supply end, the output end of the sensor is electrically connected to the grounding end, and the detection end is electrically connected to the input end of the sensor; the sensor is used for switching on or off according to whether the sample container inserted into the hole site is detected; the detection processing unit is used for supplying power to the sensor through the power supply end and detecting the level state of the input end of the sensor through the detection end.
5. The sensor matrix-based sample detection plate according to claim 4, wherein the number of the sensors is plural, the plural sensors being arranged in a matrix manner, wherein input terminals of the sensors of the same row number are connected in parallel to power supply terminals corresponding to the same row number; and the input ends of the sensors with the same column number are electrically connected with the detection ends corresponding to the same column number.
6. The sensor matrix-based sample detection plate according to claim 5, wherein the sensors are reflective photo-couplers comprising a light emitting section and a photoelectric conversion section; wherein an input terminal of the light emitting section and an input terminal of the photoelectric conversion section are electrically connected and serve as an input terminal of the reflective photoelectric coupler; the output terminal of the light emitting section and the output terminal of the photoelectric conversion section are electrically connected and serve as the output terminal of the reflective photoelectric coupler.
7. The sensor matrix-based sample detection plate of claim 5, wherein said sensor matrix-based sample detection plate further comprises: the detection trigger unit is electrically connected with the detection processing unit and is used for detecting whether the sample rack is used or not and generating a detection trigger signal to the detection processing unit after the sample rack is used, wherein the detection trigger signal is used for indicating the detection processing unit to acquire the level state of the input end of each sensor in the plurality of sensors.
8. A sample testing method applied to the sensor matrix-based sample testing plate according to any one of claims 1 to 7, said sensor matrix-based sample testing plate being placed at the bottom of said sample holder, said method comprising:
after the sensor is powered on, acquiring a detection result of the sensor;
and determining the taking state of the sample on the sample rack according to the detection result.
9. The method for testing a sample according to claim 8, wherein before obtaining the test result of the sensor, the method further comprises:
receiving a detection trigger signal;
and powering on the sensor according to the detection trigger signal.
10. The method for detecting a sample according to claim 8, wherein, in the case where the sensor matrix-based sample detection plate is the sensor matrix-based sample detection plate according to claim 5, acquiring the detection result of the sensor after the sensor is powered on includes:
powering on a row of sensors with the same row number in the plurality of sensors through the power supply terminal;
detecting the level state of the input end of the sensor with different column numbers in the row of sensors through the detection end, and taking the level state as the detection result of the sensor corresponding to the row-column intersection point; wherein a high level in the level state indicates that no sample container is inserted in the well site corresponding to the sensor, and a low level in the level state indicates that a sample container is inserted in the well site corresponding to the sensor.
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