CN111249172A - Beauty injection gel and preparation method thereof - Google Patents
Beauty injection gel and preparation method thereof Download PDFInfo
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- CN111249172A CN111249172A CN202010042735.6A CN202010042735A CN111249172A CN 111249172 A CN111249172 A CN 111249172A CN 202010042735 A CN202010042735 A CN 202010042735A CN 111249172 A CN111249172 A CN 111249172A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/733—Alginic acid; Salts thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/736—Chitin; Chitosan; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/91—Injection
Abstract
The invention discloses a beauty injection gel and a preparation method thereof, relating to the field of medical beauty, aiming at solving the problems of low biocompatibility, short gel degradation period, poor slow release effect and poor long-term stability of the existing beauty injection gel. Several of the above materials are mixed together before use. The invention carries out secondary coating on substances such as hyaluronic acid, fish glue and the like to form a compact coating layer, can realize slow release of the substances and achieves the effect of delaying the enzymolysis of the hyaluronic acid. The product of the invention has good safety, high viscoelasticity, can stably exist in the intradermal tissues and the subcutaneous tissues for a long time, has lasting repairing effect, does not have hyperplasia products and is easy to take out. The invention is applied to the field of beauty injection gel.
Description
Technical Field
The invention relates to the field of medical cosmetology, in particular to a cosmetology injection gel and a preparation method thereof.
Background
In recent years, with the advancement of technology and the improvement of living standards, the awareness and the demand for facial beauty treatment have been relatively increased. Due to fear of pain and the influence of long recovery period on living work, people need a beauty treatment with small wound, small pain, quick recovery and long effective period, and the injection of beauty treatment is a beauty treatment method for injecting substances to local parts of human bodies to correct skin defects. The key of injection beauty is injection materials, and at present, collagen and hyaluronic acid materials are commonly used in common beauty injection materials.
Hyaluronic acid is a natural polysaccharide, a transparent and viscous colloidal substance, and is widely found in connective tissues, skin dermis, articular cartilage, placenta and other tissues of vertebrates in nature. It can be well fused with human skin tissue, and can help human connective tissue to absorb and lock water molecules by hydration with protein, so as to improve the metabolic environment of cells and balance of water and ions, thereby increasing skin elasticity and achieving the effect of removing wrinkles. Although it has the advantages of no toxicity, no immunity, good biocompatibility, normal temperature storage and the like, it can be decomposed and absorbed by human body, and the wrinkle-removing effect has longer maintenance time than that of bovine collagen, but the wrinkle-removing material is not permanent. Also, collagen can be absorbed or degraded by the human body, so the wrinkle-removing effect can be maintained for only several months.
Collagen and hyaluronic acid are metabolized in vivo, have short retention time and require multiple injections for a long time. In order to increase the retention time, the metabolic problem is currently reduced at home and abroad by cross-linking hyaluronic acid. The crosslinked hyaluronic acid obtained by modifying divinyl sulfone or 1, 4-butanediol diglycidyl ether serving as a crosslinking agent is most widely applied, the degradation time of the crosslinked hyaluronic acid in a human body is generally 12-18 months, and the degradation time of uncrosslinked hyaluronic acid in the human body is generally only 6 months. In order to seek higher degradation time in human body, the introduction amount of the cross-linking agent is increased, however, the cross-linking agent has high biological toxicity, and potential danger is caused to injection beauty.
Therefore, a material for injection beauty is needed, which not only has good biocompatibility, but also can exist for a long time, and the material has a very important significance for injection beauty.
Disclosure of Invention
The invention aims to solve the problems of low biocompatibility, short gel degradation period, poor slow release effect and poor long-term stability of the existing beauty injection gel.
The invention relates to a beauty injection gel, which consists of a component A, a component B and a component C; the mass ratio of the component A to the component B to the component C is 1: 10-15: 1-1.5; the component A is prepared from chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1 to 0.5 percent; the component B is prepared from hyaluronic acid, fish gelatin, vitamin C and normal saline in a mass ratio of 1-5: 6-8: 12-15: 80-90; the component C is an alginate solution with the mass percentage of 2-3%.
The invention relates to a preparation method of a beauty injection gel, which is realized according to the following steps:
firstly, mixing chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1-0.5, and mixing under stirring to obtain a chitosan solution as a component A;
mixing hyaluronic acid, fish gelatin, vitamin C and normal saline, wherein the mass ratio of the hyaluronic acid to the fish gelatin to the vitamin C to the normal saline is (1-5): 6-8: 12-15: 80-90 as component B;
dissolving alginate in water to prepare an alginate solution with the mass percentage of 2-3% as a component C;
and fourthly, respectively packaging the component A, the component B and the component C, and sterilizing to obtain the beauty injection gel.
The invention relates to a beauty injection gel, which consists of a component A, a component B and a component C; the mass ratio of the component A to the component B to the component C to the component D is 1: 10-15: 1-1.5: 1-2; the component A is prepared from chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1 to 0.5 percent; the component B is prepared from fish gelatin, vitamin C and normal saline in a mass ratio of 6-8: 12-15: 80-90; the component C is an alginate solution with the mass percentage of 2-3%; the component D is hyaluronic acid and xanthan gum mixed gel.
The invention relates to a preparation method of a beauty injection gel, which is realized according to the following steps:
firstly, mixing chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1-0.5, and mixing under stirring to obtain a chitosan solution as a component A;
mixing fish gelatin, vitamin C and normal saline, wherein the mass ratio of hyaluronic acid to fish gelatin to vitamin C to normal saline is (1-5): 6-8: 12-15: 80-90 as component B;
dissolving alginate in water to prepare an alginate solution with the mass percentage of 2-3% as a component C;
adding the modified hyaluronic acid gel into the xanthan gum dispersion, hybridizing for 24 hours at room temperature, and sterilizing to obtain hyaluronic acid and xanthan gum mixed gel; as component D;
fifthly, packaging the component A, the component B, the component C and the component D respectively, and sterilizing to obtain the beauty injection gel; the volume ratio of the modified sodium hyaluronate gel to the xanthan gum dispersion is 1.5-2.5: 1.
The invention has the following beneficial effects:
according to the invention, substances such as hyaluronic acid, fish gelatin and the like are coated for the second time to form a compact coating layer, so that the hyaluronic acid can be slowly released, and the effect of delaying the enzymolysis of hyaluronic acid is achieved. The hyaluronic acid is not crosslinked, so that the hyaluronic acid has no toxic effect on human bodies and has good biocompatibility.
According to the invention, a layer of alginate is sprayed on the surface of the chitosan coated with hyaluronic acid, fish glue and other substances, the alginate and the hyaluronic acid can form a uniform and breathable moisture retention layer, and hydroxyl groups on molecules of the alginate and the hyaluronic acid can effectively combine with water molecules, so that the loss of water is reduced. The hyaluronic acid is dripped into the chitosan solution through the dropper, the condensed hyaluronic acid solution can form micro-particles, and the chitosan and the hyaluronic acid can be effectively coated together during continuous stirring because the chitosan has certain viscosity. Can slowly release substances such as hyaluronic acid, fish gelatin and the like, and has ideal slow release effect.
The degradation period of the beauty injection gel in vivo is more than 12 months.
The xanthan gum selected by the invention has good biocompatibility and is difficult to metabolize and degrade in a human body; the invention adopts xanthan gum with the molecular weight of 1000-2000 ten thousand and the viscosity of 1000-2000 Pa.s, the viscoelasticity is high, the stability is better, the xanthan gum with large molecular weight and high viscosity is not good in fluidity, but the fluidity is improved after the xanthan gum is hybridized with the modified sodium hyaluronate, and the xanthan gum is more beneficial to injection use.
The method has mild reaction conditions and is easy for large-scale production. The prepared product is used for injecting a cosmetic filling material, has the effects of improving fine lines, wrinkles and skin depressions, has good safety and high viscoelasticity, can stably exist in intradermal tissues and subcutaneous tissues for a long time, has a lasting repairing effect, does not contain hyperplasia products, and is easy to take out.
Detailed Description
The first embodiment is as follows: the beauty injection gel of the embodiment is composed of a component A, a component B and a component C; the mass ratio of the component A to the component B to the component C is 1: 10-15: 1-1.5; the component A is prepared from chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1 to 0.5 percent; the component B is prepared from hyaluronic acid, fish gelatin, vitamin C and normal saline in a mass ratio of 1-5: 6-8: 12-15: 80-90; the component C is an alginate solution with the mass percentage of 2-3%.
The second embodiment is as follows: the first difference between the present embodiment and the specific embodiment is: the emulsifier is fatty acid monoglyceride, lauric acid monoglyceride or sorbitol ester. The rest is the same as the first embodiment.
The third concrete implementation mode: the first difference between the present embodiment and the specific embodiment is: the alginate is sodium alginate or potassium alginate. The rest is the same as the first embodiment.
The fourth concrete implementation mode: the first difference between the present embodiment and the specific embodiment is: it is composed of a component A, a component B and a component C; the mass ratio of the component A to the component B to the component C is 1: 10-12: 1-1.5; the component A is prepared from chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.2-0.4: 8-10: 0.1 to 0.5 percent; the component B is prepared from hyaluronic acid, fish gelatin, vitamin C and normal saline in a mass ratio of 1-4: 6-8: 12-14: 80-90; the component C is an alginate solution with the mass percentage of 3 percent. The rest is the same as the first embodiment.
The fifth concrete implementation mode: the preparation method of the beauty injection gel of the embodiment is realized according to the following steps:
firstly, mixing chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1-0.5, and mixing under stirring to obtain a chitosan solution as a component A;
mixing hyaluronic acid, fish gelatin, vitamin C and normal saline, wherein the mass ratio of the hyaluronic acid to the fish gelatin to the vitamin C to the normal saline is (1-5): 6-8: 12-15: 80-90 as component B;
dissolving alginate in water to prepare an alginate solution with the mass percentage of 2-3% as a component C;
and fourthly, respectively packaging the component A, the component B and the component C, and sterilizing to obtain the beauty injection gel.
The sixth specific implementation mode: the fifth embodiment is different from the fifth embodiment in that: the deacetylation degree of the chitosan is 95 percent; the hyaluronic acid is sodium hyaluronate, and the intrinsic viscosity of the hyaluronic acid is 2-2.8 m3In terms of/kg. The rest is the same as the fifth embodiment.
The seventh embodiment: the fifth embodiment is different from the fifth embodiment in that: the emulsifier is fatty acid monoglyceride, lauric acid monoglyceride or sorbitol ester. The rest is the same as the fifth embodiment.
The specific implementation mode is eight: the fifth embodiment is different from the fifth embodiment in that: the alginate is sodium alginate or potassium alginate. The rest is the same as the fifth embodiment.
The specific implementation method nine: the embodiment relates to a beauty injection gel, which consists of a component A, a component B and a component C; the mass ratio of the component A to the component B to the component C to the component D is 1: 10-15: 1-1.5: 1-2; the component A is prepared from chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1 to 0.5 percent; the component B is prepared from fish gelatin, vitamin C and normal saline in a mass ratio of 6-8: 12-15: 80-90; the component C is an alginate solution with the mass percentage of 2-3%; the component D is hyaluronic acid and xanthan gum mixed gel.
The detailed implementation mode is ten: the present embodiment differs from the ninth embodiment in that: the emulsifier is fatty acid monoglyceride, lauric acid monoglyceride or sorbitol ester. The rest is the same as the embodiment nine.
The concrete implementation mode eleven: the present embodiment differs from the ninth embodiment in that: the alginate is sodium alginate or potassium alginate. The rest is the same as the embodiment nine.
The specific implementation mode twelve: the present embodiment differs from the ninth embodiment in that: it is composed of a component A, a component B and a component C; the mass ratio of the component A to the component B to the component C to the component D is 1: 10-12: 1-1.5: 1-2; the component A is prepared from chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.4: 8-10: 0.1 to 0.5 percent; the component B is prepared from fish gelatin, vitamin C and normal saline in a mass ratio of 6-8: 12-14: 80-90; the component C is an alginate solution with the mass percentage of 2-3%; the component D is hyaluronic acid and xanthan gum mixed gel. The rest is the same as the embodiment nine.
The specific implementation mode is thirteen: the preparation method of the beauty injection gel of the embodiment is realized according to the following steps:
firstly, mixing chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1-0.5, and mixing under stirring to obtain a chitosan solution as a component A;
mixing fish gelatin, vitamin C and normal saline, wherein the mass ratio of hyaluronic acid to fish gelatin to vitamin C to normal saline is (1-5): 6-8: 12-15: 80-90 as component B;
dissolving alginate in water to prepare an alginate solution with the mass percentage of 2-3% as a component C;
adding the modified hyaluronic acid gel into the xanthan gum dispersion, hybridizing for 24 hours at room temperature, and sterilizing to obtain hyaluronic acid and xanthan gum mixed gel; as component D;
fifthly, packaging the component A, the component B, the component C and the component D respectively, and sterilizing to obtain the beauty injection gel; the volume ratio of the modified sodium hyaluronate gel to the xanthan gum dispersion is 1.5-2.5: 1.
The specific implementation mode is fourteen: the present embodiment is different from the specific embodiment by thirteen: the preparation method of the modified hyaluronic acid gel comprises the following steps:
adding sodium hyaluronate dry powder into a lysine solution, uniformly mixing, standing at 4 ℃ for 12 hours, then adding a 4- (4, 6-dimethoxytriazine-2-yl) -4-methylmorpholine hydrochloride solution, uniformly mixing, reacting at 4 ℃ for 48-72 hours, dialyzing by adopting a PBS (phosphate buffer solution), changing the PBS buffer solution for 1 time every 2 hours, stopping dialysis after 60 hours, crushing and granulating to obtain modified sodium hyaluronate gel;
in the first step, the molar ratio of the sodium hyaluronate to the lysine to the 4- (4, 6-dimethoxytriazine-2-yl) -4-methylmorpholine salt is 8-12: 1:1.
The other steps are the same as those in the thirteenth embodiment.
The concrete implementation mode is fifteen: the present embodiment is different from the specific embodiment in the fourteenth aspect: the molecular weight of the sodium hyaluronate is 200-400 million daltons, the molecular weight of the xanthan gum is 1000-2000 million, and the viscosity is 1000-2000 Pa.s. The rest is the same as the embodiment fourteen.
The specific implementation mode is sixteen: the present embodiment is different from the specific embodiment in the fourteenth aspect: the mass concentration of the xanthan gum dispersion is 8-10 g/L. The rest is the same as the embodiment fourteen.
Seventeenth embodiment: the present embodiment is different from the specific embodiment in the fourteenth aspect: the pH value of the lysine solution is 6-7. The rest is the same as the embodiment fourteen.
The specific implementation mode is eighteen: the present embodiment is different from the specific embodiment in the fourteenth aspect: the sterilization is carried out at 115 ℃ for 20 min. The rest is the same as the embodiment fourteen.
The detailed embodiment is nineteen: the present embodiment is different from the specific embodiment by thirteen: the emulsifier is fatty acid monoglyceride, lauric acid monoglyceride or sorbitol ester. The other steps are the same as those in the thirteenth embodiment.
The specific implementation mode twenty: the present embodiment is different from the specific embodiment by thirteen: the alginate is sodium alginate or potassium alginate. The other steps are the same as those in the thirteenth embodiment.
The beneficial effects of the present invention are demonstrated by the following examples:
example 1
The beauty injection gel of the embodiment is composed of a component A, a component B and a component C; the mass ratio of the component A to the component B to the component C is 1:10: 1.5; the component A is prepared from chitosan, acetic acid, water and lauric acid monoglyceride in a mass ratio of 1: 0.4: 10: 0.1 mixing; the component B is prepared from hyaluronic acid, fish gelatin, vitamin C and normal saline in a mass ratio of 1: 8: 10: 80; the component C is a sodium alginate solution with the mass percentage of 2-3%;
the preparation method of the beauty injection gel comprises the following steps:
mixing chitosan, acetic acid, water and lauric acid monoglyceride according to the above proportion under stirring to obtain a chitosan solution as a component A;
secondly, mixing hyaluronic acid, fish gelatin, vitamin C and normal saline according to the proportion to obtain a component B;
dissolving sodium alginate in water to prepare a sodium alginate solution with the mass percentage of 3 percent, and obtaining a component C;
fourthly, respectively packaging the component A, the component B and the component C, and obtaining the beauty injection gel after irradiation sterilization;
before use, the component B is dripped into the component A through a dropper, the component B is stirred while being added, and the component C is sprayed on the surfaces of the component A and the component B to obtain gel for injection.
The cosmetic injection gel prepared in this example was subjected to the following performance tests:
1) and (3) testing the degradation performance:
a helicase is selected for a degradation experiment, the helicase is used as a compound enzyme and has high degradation efficiency, and the helicase is dissolved in a buffer solution with the pH value of 4.0 to prepare a helicase solution with the concentration of 1.5 mg/mL. Cutting the beauty injection gel obtained in the embodiment into gel block samples with the diameter of 20mm and the thickness of 4mm, adding the gel block samples into a glass beaker, adding a helicase solution to immerse the beauty injection gel, testing the time for the three-dimensional network structure of the beauty injection gel to be damaged and degraded to be dissolved under the condition of constant-temperature water bath at 37 ℃, updating the helicase solution every 48h, and observing that the beauty injection gel is completely degraded and dissolved in water.
The test results in 65h of complete degradation time for the cosmetic injection gel of this example.
2) And (3) testing mechanical properties:
the mechanical property of the beauty injection gel is measured by using a HAAKE rotational rheometer, a sample of the beauty injection gel is made into a round cake shape with the diameter of 30mm, the shearing frequency range is 0.1 to 10Hz, and the temperature is controlled at 25 ℃. The storage shear modulus of the cosmetic injection gel obtained in the example is 1930 Pa.
3) Skin irritation test:
1.0mL of the cosmetic injection gel obtained in example was injected under the skin of the back of 20 rats, and the rats were sacrificed after 15 days, and it was observed that the implanted gel did not spread, had no adhesion to the peripheral tissues, and had no abnormality in the peripheral tissues. No erythema or edema was observed at the skin injection site. The beautifying injection gel is proved to be mild and non-toxic, and can not generate obvious stimulation and anaphylactic reaction to skin.
4) In vivo degradation testing:
the cosmetic injection gel obtained in example was injected under the skin of the back of 20 rats to form a round bulge, the skin still bulged after 18 days, the degradation started in 30 days, and the gel was completely degraded by 39 days.
The beauty injection gel obtained in the embodiment swells when meeting water, but does not dissolve in water, and the gel body is clear and transparent.
The degradation period of the beauty injection gel obtained in the embodiment in vivo is more than 12 months.
Example 2
The beauty injection gel of the embodiment is composed of a component A, a component B and a component C; the mass ratio of the component A, the component B, the component C and the component D is 1:10:1: 1.5; the component A is prepared from chitosan, acetic acid, water and sorbitol ester according to a mass ratio of 1: 0.4: 10: 0.1 mixing; the component B is prepared from fish gelatin, vitamin C and normal saline in a mass ratio of 8: 10: 80; the component C is alginate solution with the mass percentage content of 2 percent; the component D is hyaluronic acid and xanthan gum mixed gel;
before use, the component B is dripped into the component A through a dropper, the component D is added into the mixture of the component B and the component A while stirring, and the component C is sprayed on the surface of the substance to obtain gel for injection.
The preparation method of the hyaluronic acid and xanthan gum mixed gel comprises the following steps:
firstly, adding sodium hyaluronate dry powder into a lysine solution, uniformly mixing, standing at 4 ℃ for 12 hours, then adding a DMTMM (4- (4, 6-dimethoxytriazine-2-yl) -4-methylmorpholine hydrochloride) solution, uniformly mixing, reacting at 4 ℃ for 48-72 hours, dialyzing by adopting a PBS buffer solution, changing the PBS buffer solution for 1 time every 2 hours, stopping dialysis after 60 hours, and crushing and granulating to obtain modified sodium hyaluronate gel;
and secondly, adding the modified sodium hyaluronate gel into xanthan gum dispersion, hybridizing for 24 hours at room temperature, and sterilizing to finish the preparation of the injectable cosmetic filling material.
In the first step, the molar ratio of the sodium hyaluronate to the lysine to the DMTMM is 10:1:1.
In the step one, the molecular weight of the sodium hyaluronate is 200 ten thousand daltons.
The pH value of the lysine solution in the first step is 7.
The osmotic pressure of the PBS buffer solution in the first step is 350mOs mol/L.
And the mass concentration of the xanthan gum dispersion liquid in the second step is 8 g/L.
In the second step, the molecular weight of the xanthan gum is 1000 ten thousand, and the viscosity is 1000 Pa.s.
The volume ratio of the modified sodium hyaluronate gel to the xanthan gum dispersion in the step two is 2:1
The sterilization in the second step is carried out at 115 ℃ for 20 min.
The cosmetic injection gel prepared in example 2 was subjected to the following performance tests:
1) and (3) testing the degradation performance:
a helicase is selected for a degradation experiment, the helicase is used as a compound enzyme and has high degradation efficiency, and the helicase is dissolved in a buffer solution with the pH value of 4.0 to prepare a helicase solution with the concentration of 1.5 mg/mL. Cutting the beauty injection gel obtained in the embodiment into gel block samples with the diameter of 20mm and the thickness of 4mm, adding the gel block samples into a glass beaker, adding a helicase solution to immerse the beauty injection gel, testing the time for the three-dimensional network structure of the beauty injection gel to be damaged and degraded to be dissolved under the condition of constant-temperature water bath at 37 ℃, updating the helicase solution every 48h, and observing that the beauty injection gel is completely degraded and dissolved in water.
The test results in 70h of complete degradation time for the cosmetic injection gel of this example.
2) And (3) testing mechanical properties:
the mechanical property of the beauty injection gel is measured by using a HAAKE rotational rheometer, a sample of the beauty injection gel is made into a round cake shape with the diameter of 30mm, the shearing frequency range is 0.1 to 10Hz, and the temperature is controlled at 25 ℃. The storage shear modulus of the cosmetic injection gel obtained in the example was found to be 2050 Pa.
3) Skin irritation test:
the 1.0mL of the cosmetic injection gel obtained in example was injected under the skin of the back of 20 rats, and the rats were sacrificed after 20 days to observe that the implanted gel did not spread, did not adhere to the peripheral tissues, and had no abnormality in the peripheral tissues. No erythema or edema was observed at the skin injection site. The beautifying injection gel is proved to be mild and non-toxic, and can not generate obvious stimulation and anaphylactic reaction to skin.
4) In vivo degradation testing:
the cosmetic injection gel obtained in example 2 was injected under the skin of the back of 20 rats to form a round bulge, the skin remained bulged after 20 days, the gel was degraded after 33 days, and the gel was completely degraded after 42 days.
5) Taking out: injecting the beauty injection gel obtained in the example 2 under the back skin of 10 rats, and taking out the injection in a minimally invasive mode after 30 days, wherein the injection is successfully taken out; after the rats were sacrificed, the internal condition of the tissue after the injection was taken out was observed, and it was found that the injection was not significantly adhered to the peripheral tissue, and there was no inflammation and proliferative tissue.
The degradation period of the beauty injection gel obtained in the embodiment in vivo is more than 12 months.
It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples for carrying out the invention, and that various changes in form and details may be made therein without departing from the spirit and scope of the invention in practice.
To make the objects, aspects and advantages of the embodiments of the present invention more apparent, the following detailed description clearly illustrates the spirit of the disclosure, and any person skilled in the art, after understanding the embodiments of the disclosure, may make changes and modifications to the technology taught by the disclosure without departing from the spirit and scope of the disclosure.
The exemplary embodiments and descriptions of the present invention are provided to explain the present invention and not to limit the present invention.
Claims (10)
1. A beauty injection gel is characterized in that the beauty injection gel consists of a component A, a component B and a component C; the mass ratio of the component A to the component B to the component C is 1: 10-15: 1-1.5; the component A is prepared from chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1 to 0.5 percent; the component B is prepared from hyaluronic acid, fish gelatin, vitamin C and normal saline in a mass ratio of 1-5: 6-8: 12-15: 80-90; the component C is an alginate solution with the mass percentage of 2-3%.
2. A cosmetic injection gel according to claim 1, characterized in that the emulsifier is a fatty acid monoglyceride, a lauric acid monoglyceride or a sorbitol ester.
3. The injection gel for beauty treatment according to claim 1, wherein said alginate is sodium alginate or potassium alginate.
4. A process for the preparation of a cosmetic injection gel as claimed in claim 1, 2 or 3, characterized in that it is carried out according to the following steps:
firstly, mixing chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1-0.5, and mixing under stirring to obtain a chitosan solution as a component A;
mixing hyaluronic acid, fish gelatin, vitamin C and normal saline, wherein the mass ratio of the hyaluronic acid to the fish gelatin to the vitamin C to the normal saline is (1-5): 6-8: 12-15: 80-90 as component B;
dissolving alginate in water to prepare an alginate solution with the mass percentage of 2-3% as a component C;
and fourthly, respectively packaging the component A, the component B and the component C, and sterilizing to obtain the beauty injection gel.
5. The method for preparing a cosmetic injection gel according to claim 4, wherein the chitosan deacetylation degree is 95%; the hyaluronic acid is sodium hyaluronate, and the intrinsic viscosity of the hyaluronic acid is 2-2.8 m3/kg。
6. A beauty injection gel is characterized in that the beauty injection gel consists of a component A, a component B and a component C; the mass ratio of the component A to the component B to the component C to the component D is 1: 10-15: 1-1.5: 1-2; the component A is prepared from chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1 to 0.5 percent; the component B is prepared from fish gelatin, vitamin C and normal saline in a mass ratio of 6-8: 12-15: 80-90; the component C is an alginate solution with the mass percentage of 2-3%; the component D is hyaluronic acid and xanthan gum mixed gel.
7. A process for the preparation of a cosmetic injection gel as claimed in claim 6, characterized in that it is carried out according to the following steps:
firstly, mixing chitosan, acetic acid, water and an emulsifier according to a mass ratio of 1: 0.1-0.5: 8-12: 0.1-0.5, and mixing under stirring to obtain a chitosan solution as a component A;
mixing fish gelatin, vitamin C and normal saline, wherein the mass ratio of hyaluronic acid to fish gelatin to vitamin C to normal saline is (1-5): 6-8: 12-15: 80-90 as component B;
dissolving alginate in water to prepare an alginate solution with the mass percentage of 2-3% as a component C;
adding the modified hyaluronic acid gel into the xanthan gum dispersion, hybridizing for 24 hours at room temperature, and sterilizing to obtain hyaluronic acid and xanthan gum mixed gel; as component D;
fifthly, packaging the component A, the component B, the component C and the component D respectively, and sterilizing to obtain the beauty injection gel; the volume ratio of the modified sodium hyaluronate gel to the xanthan gum dispersion is 1.5-2.5: 1.
8. The method for preparing the beauty injection gel according to claim 7, wherein the method for preparing the modified hyaluronic acid gel comprises the following steps:
adding sodium hyaluronate dry powder into a lysine solution, uniformly mixing, standing at 4 ℃ for 12 hours, then adding a 4- (4, 6-dimethoxytriazine-2-yl) -4-methylmorpholine hydrochloride solution, uniformly mixing, reacting at 4 ℃ for 48-72 hours, dialyzing by adopting a PBS (phosphate buffer solution), changing the PBS buffer solution for 1 time every 2 hours, stopping dialysis after 60 hours, crushing and granulating to obtain modified sodium hyaluronate gel;
in the first step, the molar ratio of the sodium hyaluronate to the lysine to the 4- (4, 6-dimethoxytriazine-2-yl) -4-methylmorpholine salt is 8-12: 1:1.
9. The method for preparing injection gel for beauty treatment according to claim 7, wherein the molecular weight of the sodium hyaluronate is 200-400 ten thousand daltons, the molecular weight of the xanthan gum is 1000-2000 ten thousand, and the viscosity is 1000-2000 Pa-s.
10. The preparation method of the beauty injection gel according to claim 7, wherein the mass concentration of the xanthan gum dispersion is 8-10 g/L.
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| CN115501390A (en) * | 2022-10-18 | 2022-12-23 | 沈阳药科大学 | Skin beautifying compound injection and preparation method thereof |
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