CN111248232B - Slow-release efficient insect-repelling biological pesticide and preparation method thereof - Google Patents
Slow-release efficient insect-repelling biological pesticide and preparation method thereof Download PDFInfo
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- CN111248232B CN111248232B CN202010180049.5A CN202010180049A CN111248232B CN 111248232 B CN111248232 B CN 111248232B CN 202010180049 A CN202010180049 A CN 202010180049A CN 111248232 B CN111248232 B CN 111248232B
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- 239000000575 pesticide Substances 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- RSWGJHLUYNHPMX-UHFFFAOYSA-N Abietic-Saeure Natural products C12CCC(C(C)C)=CC2=CCC2C1(C)CCCC2(C)C(O)=O RSWGJHLUYNHPMX-UHFFFAOYSA-N 0.000 claims abstract description 28
- KHPCPRHQVVSZAH-HUOMCSJISA-N Rosin Natural products O(C/C=C/c1ccccc1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KHPCPRHQVVSZAH-HUOMCSJISA-N 0.000 claims abstract description 26
- KHPCPRHQVVSZAH-UHFFFAOYSA-N trans-cinnamyl beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OCC=CC1=CC=CC=C1 KHPCPRHQVVSZAH-UHFFFAOYSA-N 0.000 claims abstract description 26
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000000243 solution Substances 0.000 claims abstract description 23
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 20
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 20
- 238000010992 reflux Methods 0.000 claims abstract description 16
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 12
- 230000000853 biopesticidal effect Effects 0.000 claims abstract description 11
- 238000001704 evaporation Methods 0.000 claims abstract description 11
- 239000002904 solvent Substances 0.000 claims abstract description 11
- 239000003054 catalyst Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 238000000227 grinding Methods 0.000 claims abstract description 8
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 26
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- 230000000749 insecticidal effect Effects 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 10
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 241000893864 Nerium Species 0.000 claims description 4
- 239000004408 titanium dioxide Substances 0.000 claims description 3
- 241000717544 Aconitum lycoctonum subsp. vulparia Species 0.000 claims description 2
- 229910000410 antimony oxide Inorganic materials 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims description 2
- VTRUBDSFZJNXHI-UHFFFAOYSA-N oxoantimony Chemical compound [Sb]=O VTRUBDSFZJNXHI-UHFFFAOYSA-N 0.000 claims description 2
- 235000012015 potatoes Nutrition 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims 1
- 239000005871 repellent Substances 0.000 claims 1
- 230000002940 repellent Effects 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 7
- 238000010790 dilution Methods 0.000 abstract description 4
- 239000012895 dilution Substances 0.000 abstract description 4
- 241000607479 Yersinia pestis Species 0.000 abstract description 3
- RSWGJHLUYNHPMX-ONCXSQPRSA-N abietic acid Chemical compound C([C@@H]12)CC(C(C)C)=CC1=CC[C@@H]1[C@]2(C)CCC[C@@]1(C)C(O)=O RSWGJHLUYNHPMX-ONCXSQPRSA-N 0.000 abstract 2
- 244000187664 Nerium oleander Species 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 238000010298 pulverizing process Methods 0.000 description 6
- 235000013305 food Nutrition 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- 230000001846 repelling effect Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000498254 Heterodera glycines Species 0.000 description 3
- 229930013930 alkaloid Natural products 0.000 description 3
- DUEPRVBVGDRKAG-UHFFFAOYSA-N carbofuran Chemical compound CNC(=O)OC1=CC=CC2=C1OC(C)(C)C2 DUEPRVBVGDRKAG-UHFFFAOYSA-N 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 150000003797 alkaloid derivatives Chemical class 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 208000031513 cyst Diseases 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 229930182478 glucoside Natural products 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- MHVJRKBZMUDEEV-APQLOABGSA-N (+)-Pimaric acid Chemical compound [C@H]1([C@](CCC2)(C)C(O)=O)[C@@]2(C)[C@H]2CC[C@](C=C)(C)C=C2CC1 MHVJRKBZMUDEEV-APQLOABGSA-N 0.000 description 1
- MHVJRKBZMUDEEV-UHFFFAOYSA-N (-)-ent-pimara-8(14),15-dien-19-oic acid Natural products C1CCC(C(O)=O)(C)C2C1(C)C1CCC(C=C)(C)C=C1CC2 MHVJRKBZMUDEEV-UHFFFAOYSA-N 0.000 description 1
- BTXXTMOWISPQSJ-UHFFFAOYSA-N 4,4,4-trifluorobutan-2-one Chemical compound CC(=O)CC(F)(F)F BTXXTMOWISPQSJ-UHFFFAOYSA-N 0.000 description 1
- BQACOLQNOUYJCE-FYZZASKESA-N Abietic acid Natural products CC(C)C1=CC2=CC[C@]3(C)[C@](C)(CCC[C@@]3(C)C(=O)O)[C@H]2CC1 BQACOLQNOUYJCE-FYZZASKESA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- JLPDBLFIVFSOCC-UHFFFAOYSA-N Oleandrin Natural products O1C(C)C(O)C(OC)CC1OC1CC(CCC2C3(CC(C(C3(C)CCC32)C=2COC(=O)C=2)OC(C)=O)O)C3(C)CC1 JLPDBLFIVFSOCC-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- -1 alkaloid glucoside Chemical class 0.000 description 1
- RXVGBQCEAQZMLW-UHFFFAOYSA-N alpha-solanine Natural products CC1CCC2C(C)C3C(CC4C5CC=C6CC(CCC6(C)C5CCC34C)OC7OC(CO)C(O)C(OC8OC(CO)C(O)C(O)C8O)C7OC9OC(CO)C(O)C(O)C9O)N2C1 RXVGBQCEAQZMLW-UHFFFAOYSA-N 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 235000013569 fruit product Nutrition 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000006272 natural pesticide Substances 0.000 description 1
- JLPDBLFIVFSOCC-XYXFTTADSA-N oleandrin Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1C[C@@H](CC[C@H]2[C@]3(C[C@@H]([C@@H]([C@@]3(C)CC[C@H]32)C=2COC(=O)C=2)OC(C)=O)O)[C@]3(C)CC1 JLPDBLFIVFSOCC-XYXFTTADSA-N 0.000 description 1
- 229950010050 oleandrin Drugs 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- ZGVSETXHNHBTRK-OTYSSXIJSA-N solanine Chemical compound O([C@H]1[C@@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@@H]1[C@@H]([C@H](O)[C@@H](O)[C@H](C)O1)O)O[C@@H]1CC2=CC[C@H]3[C@@H]4C[C@@H]5N6C[C@@H](C)CC[C@@H]6[C@H]([C@@H]5[C@@]4(C)CC[C@@H]3[C@@]2(C)CC1)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O ZGVSETXHNHBTRK-OTYSSXIJSA-N 0.000 description 1
- 229940031352 solanine Drugs 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/18—Euphorbiaceae [Spurge family], e.g. ricinus [castorbean]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the field of agricultural products, and discloses a slow-release efficient insect-repelling biological pesticide and a preparation method thereof, wherein the preparation method comprises the steps of extracting oleander leaves and radix euphorbiae lantu roots with acid-alcohol solution in a refluxing manner to obtain solution A; adding germinated potato extract and Colophonium into solution A, stirring to dissolve, and evaporating solvent to obtain Colophonium; heating the desinsection rosin and a catalyst to 200-280 ℃ under the protection of nitrogen for reaction, cooling to room temperature, and mechanically grinding and crushing in liquid nitrogen to obtain the slow-release efficient desinsection biopesticide. After the slow-release efficient insect-repelling biological pesticide prepared by the invention is applied, soil pests of crops can be efficiently avoided for a long time, the organic pollution to the surrounding environment is not easy to cause, and the dilution by rainwater can be effectively avoided.
Description
Technical Field
The invention relates to the field of agricultural products, in particular to a slow-release efficient insect-repelling biological pesticide and a preparation method thereof.
Background
The prior art is mainly used for killing insects and bacteria by chemical pesticides, has low pesticide effect and long residual toxicity period, seriously influences the growth environment of crops and the health of people and livestock, can prevent and treat diseases and insect pests of agriculture and forestry, and can also cause harm to people and livestock. And due to the large amount of chemical pesticides applied, air, water source, soil and food are polluted, and poisons are accumulated in livestock and human bodies to cause poisoning.
The pesticide utilization rate is generally 10% and the balance of about 90% remains in the environment, causing environmental pollution. A large amount of pesticides volatilize into air, flow into water, settle and gather in soil, pollute farm animals and fish fruit products, and are transferred into human bodies through the enrichment function of a food chain to cause harm to the human bodies. The highly toxic pesticide has high efficiency and high toxicity, and can be left in the environment for a long time, so that when people and livestock eat food containing the residual pesticide, cumulative poisoning can be caused. The harm is usually displayed after long-term accumulation, and the disease enters a human body through the enrichment effect of a food chain, so that the harm is difficult to find in time and serious.
Once the pesticide enters the environment, the toxicity and high residue characteristics of the pesticide can have effects, so that serious pollution to the atmosphere, water and soil is caused, and harm is brought to human health. When toxic pesticides are applied to crops, vegetables and fruit trees, the residual pesticides on the surface of the crops are easy to permeate into the waxy layer of the surface of the crops due to strong lipid solubility, so that the pesticides are difficult to completely clean.
Disclosure of Invention
In view of the above, the present invention aims to provide a slow-release efficient biopesticide and a preparation method thereof, wherein the biopesticide can efficiently repel soil pests of crops for a long time after being applied, is not easy to cause organic pollution to the surrounding environment, and can effectively avoid rainwater dilution.
In order to solve the technical problems, the invention provides a slow-release efficient insect-repelling biological pesticide and a preparation method thereof, wherein the preparation method comprises the following steps:
s1, drying and crushing oleander leaves and radix euphorbiae lantu, and extracting with an acid-alcohol solution under reflux to obtain a solution A;
s2, crushing and drying the germinated potatoes, performing reflux extraction with ethanol, and evaporating a solvent to obtain a potato extract;
s3, adding the potato extract and the rosin into the solution A, fully stirring and dissolving, and evaporating the solvent to obtain the insecticidal rosin;
s4, mixing the desinsection-repelling rosin with a catalyst, heating to 200-280 ℃ under the protection of nitrogen for reaction, cooling to room temperature, and mechanically grinding and crushing in liquid nitrogen to obtain the slow-release efficient desinsection-repelling biological pesticide.
Preferably, in step S1, the proportion of the oleander leaves, the roots of the Chinese wolfsbane and the acid-alcohol solution is (10-20) g, (16-40) g:50ml, wherein the volume ratio of acetic acid to ethanol in the acid-alcohol solution is 1: 4-9.
Preferably, in the step S1, the reflux temperature is 60-80 ℃, and the reflux time is 4-5 h.
Preferably, in step S2, the ratio of the sprouted potato to the ethanol is (200-240) g:50 ml.
Preferably, in the step S2, the reflux temperature is 70-80 ℃, and the reflux time is 2-4 h.
Preferably, in step S3, the ratio of the potato extract, the rosin and the liquid A is (0.5-1) g (35-40) g:100 ml.
Preferably, in step S4, the mass ratio of the rosin for repelling insects to the catalyst is 20000 to 50000:1, and the catalyst is one of titanium dioxide and antimony oxide.
Preferably, in step S4, the reaction pressure is 40 to 60Pa, and the reaction time is 6 to 12 hours.
Preferably, in the step S4, the grinding fineness of the slow-release efficient insect-repelling biological pesticide is controlled to be 2000-2500 meshes.
The invention also provides the slow-release high-efficiency insect-repelling biological pesticide prepared by the preparation method.
Compared with the prior art, the invention has the following beneficial effects:
1) the invention esterifies resin acid (abietic acid, pimaric acid), a small amount of fatty acid, rosin anhydride and other acid substances in rosin with hydroxyl on alkaloid glucoside, so that the active alkaloid insecticide is cross-linked and wrapped in the regenerated rosin, the hardness of the regenerated rosin is improved by using liquid nitrogen, and the regenerated rosin is mechanically ground to a proper fineness, thereby ensuring that the biopesticide is kept released in a specific crop growth period. And the esterified and crosslinked biological pesticide is more resistant to rainwater dilution.
2) The oleandrin, the radix euphorbiae lantu flower extract, the solanine and the rosin are all pure natural pesticides, belong to biological pesticides and do not bring organic pollution to crops. Due to the synergistic effect of various insecticidal active substances, the killing effect is better.
Detailed Description
The invention will be better understood from the following examples. However, those skilled in the art will readily appreciate that the description of the embodiments is only for illustrating the present invention and should not be taken as limiting the invention as detailed in the claims.
Example 1
A slow-release high-efficiency biological pesticide for repelling insects and a preparation method thereof comprise the following steps:
1. drying and pulverizing folium Nerii 30g and radix Euphorbiae Fischerianae 60g, and reflux-extracting with 100ml acid-alcohol solution of acetic acid and ethanol at a volume ratio of 1:5 at 70 deg.C for 4.5 hr to obtain solution A;
2. pulverizing 460g germinated potato, sun drying, extracting with 100ml ethanol under reflux at 75 deg.C for 3 hr, and evaporating solvent to obtain potato extract;
3. adding 0.6g of potato extract and 38g of rosin into 100ml of the solution A, fully stirring and dissolving, and evaporating the solvent to obtain insecticidal rosin;
4. mixing 30g of desinsection rosin and 1mg of titanium dioxide, heating to 270 ℃ under the protection of 50Pa nitrogen for reaction for 10 hours, cooling to room temperature, mechanically grinding in liquid nitrogen and crushing to 2400 meshes to prepare the slow-release efficient desinsection biopesticide.
Example 2
A slow-release high-efficiency biological pesticide for repelling insects and a preparation method thereof comprise the following steps:
1. drying and pulverizing 20g folium Nerii and 32g radix Euphorbiae Fischerianae, and reflux-extracting with 100ml acid-alcohol solution of acetic acid and ethanol at volume ratio of 1:4 at 60 deg.C for 4 hr to obtain solution A;
2. pulverizing 400g of germinated potato, sun drying, extracting with 100ml of ethanol at 70 deg.C under reflux for 2 hr, and evaporating solvent to obtain potato extract;
3. adding 0.3g of potato extract and 21g of rosin into 60ml of the solution A, fully stirring and dissolving, and evaporating the solvent to obtain insecticidal rosin;
4. mixing 20g of desinsection rosin and 1mg of catalyst, heating to 200 ℃ under the protection of nitrogen gas of 40Pa for reaction for 6h, cooling to room temperature, mechanically grinding in liquid nitrogen and crushing to 2000 meshes to prepare the slow-release efficient desinsection biopesticide.
Example 3
A slow-release high-efficiency biological pesticide for repelling insects and a preparation method thereof comprise the following steps:
1. drying and pulverizing 40g folium Nerii and 80g radix Euphorbiae Fischerianae, and reflux-extracting with 100ml acid-alcohol solution of acetic acid and ethanol at volume ratio of 1:9 at 80 deg.C for 5 hr to obtain solution A;
2. pulverizing 480g germinated potato, sun drying, extracting with 100ml ethanol at 80 deg.C under reflux for 4 hr, and evaporating solvent to obtain potato extract;
3. adding 0.5g of potato extract and 20g of rosin into 50ml of the solution A, fully stirring and dissolving, and evaporating the solvent to obtain insecticidal rosin;
4. mixing 20g of desinsection rosin and 0.4mg of catalyst, heating to 280 ℃ under the protection of 60Pa nitrogen for reaction for 12h, cooling to room temperature, and mechanically grinding in liquid nitrogen to 2500 meshes to prepare the slow-release efficient desinsection biopesticide.
Plot experiment for controlling soybean cyst nematode by biological pesticide
Area per cell is 100m2The biopesticides of examples 1-3 were applied 250g per cell, respectively, and the control agent was 750g of 3% carbofuran (carbofuran) granules per cell, and a no-application blank (CK) was applied, each treatment was repeated 3 times and randomized. Firstly, rake the ground evenly and then start half a step, 100m2In a small area, 9 small ditches are opened in the small area, biopesticide or contrast agent is uniformly scattered in the ditches, soybean seeds are scattered in the ditches where the agent is spread, finally, soil covering and treading are carried out, and watering is carried out on the next day. Sampling and investigating are carried out every month, 10 cysts are randomly taken from each cell, the number of cysts of each plant is recorded, statistical analysis is carried out, the prevention effect is calculated, and the test result is shown in table 1.
TABLE 1 field plot test results of biopesticides for controlling soybean cyst nematode disease
As can be seen from Table 1, the slow-release high-efficiency insecticidal biological pesticide prepared by the embodiment of the invention has a good inhibition effect on soybean cyst nematodes, and particularly has a better continuous control effect compared with a control medicament. The control medicament is a non-sustained release medicament, the control effect is good initially, but the control effect is obviously reduced along with the influence of time and rainwater dilution. Moreover, carbofuran is a highly toxic pesticide, can be killed by birds and insects when eaten by mistake, and is likely to pollute surface water and underground water under the action of rainwater, thereby causing more serious environmental problems. The active substances of the biological pesticide are all derived from plants, are mainly glucoside of alkaloid, can be metabolized and degraded by soil microorganisms in a short time after being released, and cannot cause environmental problems.
The invention provides a slow-release efficient insect-repelling biological pesticide, a preparation method and an application concept and method thereof, and a plurality of methods and ways for realizing the technical scheme are provided, the above description is only a preferred embodiment of the invention, and it should be noted that for a person skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the invention, and the improvements and decorations should also be regarded as the protection scope of the invention. All the components not specified in the present embodiment can be realized by the prior art.
Claims (7)
1. A preparation method of a slow-release high-efficiency insecticidal biopesticide is characterized by comprising the following steps:
s1, drying and crushing oleander leaves and radix euphorbiae lantu, and extracting with an acid-alcohol solution under reflux to obtain a solution A; the proportion of the oleander leaves, the roots of the Chinese wolfsbane flowers and the acid-alcohol solution is (10-20), (16-40) g:50ml, wherein the acid-alcohol solution is a mixed solution of acetic acid and ethanol, and the volume ratio of the acetic acid to the ethanol in the acid-alcohol solution is 1: 4-9;
s2, crushing and drying the germinated potatoes, performing reflux extraction with ethanol, and evaporating a solvent to obtain a potato extract;
s3, adding the potato extract and the rosin into the solution A, fully stirring and dissolving, and evaporating the solvent to obtain the insecticidal rosin; the proportion of the potato extract, the rosin and the A liquid is (0.5-1) g, (35-40) g:100 ml;
s4, mixing the desinsection-repelling rosin with a catalyst, heating to 200-270 ℃ under the protection of nitrogen for reaction, cooling to room temperature, and mechanically grinding and crushing in liquid nitrogen to prepare the slow-release efficient desinsection-repelling biological pesticide; the mass ratio of the insecticidal and repellent rosin to the catalyst is 20000-50000: 1, and the catalyst is one of titanium dioxide and antimony oxide.
2. The method according to claim 1, wherein in step S1, the reflux temperature is 60-80 ℃ and the reflux time is 4-5 h.
3. The method according to claim 1, wherein in step S2, the ratio of the sprouted potato to the ethanol is (200-240) g:50 ml.
4. The method according to claim 1, wherein in step S2, the reflux temperature is 70-80 ℃ and the reflux time is 2-4 h.
5. The method according to claim 1, wherein in step S4, the reaction pressure is 40-60 Pa, and the reaction time is 6-12 h.
6. The preparation method according to claim 1, wherein in step S4, the grinding fineness of the slow-release efficient insecticidal and biological pesticide is controlled to 2000-2500 meshes.
7. The slow-release high-efficiency insecticidal biological pesticide prepared by the preparation method according to any one of claims 1 to 6.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104957183A (en) * | 2015-06-17 | 2015-10-07 | 湖州道场污水处理有限公司 | Environment-friendly insecticide with nerium indicum and radix euphorbiae lantu |
| CN106561750A (en) * | 2016-10-30 | 2017-04-19 | 贵州省蕙安农业科技发展有限公司 | Special vegetable insecticide for honeysuckle planting and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104957183A (en) * | 2015-06-17 | 2015-10-07 | 湖州道场污水处理有限公司 | Environment-friendly insecticide with nerium indicum and radix euphorbiae lantu |
| CN106561750A (en) * | 2016-10-30 | 2017-04-19 | 贵州省蕙安农业科技发展有限公司 | Special vegetable insecticide for honeysuckle planting and preparation method thereof |
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