CN111242538B - Dynamic whole-flow cell inventory transfer method and system - Google Patents
Dynamic whole-flow cell inventory transfer method and system Download PDFInfo
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Abstract
The invention belongs to the technical field of data transfer, and particularly relates to a dynamic whole-flow cell inventory transfer method and a system; the dynamic whole-flow cell inventory transfer method comprises the following steps: s1: receiving cell data of stock transfer, and calling a stock transfer form after receiving the cell data; s2: and acquiring inventory transfer demand data, and calling a formal library form and a temporary library form which are associated with the inventory transfer form according to the inventory transfer data and the cell data. The invention provides a new dynamic whole-flow cell inventory transfer method and a system, which can be used for quickly and accurately carrying out inventory transfer of cells based on comprehensive judgment of various factors, and the inventory transfer is not completed in a specific module, but is dynamically inserted in each link where the inventory transfer operation is likely to occur.
Description
Technical Field
The invention belongs to the technical field of data transfer, and particularly relates to a dynamic whole-flow cell inventory transfer method and system.
Background
In the process of culturing cells, the cells have the requirements of storing different generations into different entity libraries (liquid nitrogen tanks, ultralow temperature refrigerators and 4 ℃ refrigerators). That is, when cells exist in various forms (generations) during the culture, such as seed cells, working cells, etc. If the seed cells need to be stored for a long time, the seed cells need to be temporarily stored in an ultralow temperature refrigerator before being formally stored in liquid nitrogen with the storage environment of-196 ℃ for waiting for the relevant quality inspection results. This transfer between different physical environments is called cell inventory transfer. In the past, inventory transfer of a warehouse system is adopted, and the inventory transfer method has fewer considered factors and cannot realize quick and accurate inventory transfer.
Disclosure of Invention
In order to solve the technical problems, the invention provides a novel dynamic whole-flow cell inventory transfer method and system.
The specific technical scheme of the invention is as follows:
the invention provides a dynamic whole-flow cell inventory transfer method, which comprises the following steps:
s1: receiving cell data of stock transfer, and calling a stock transfer form after receiving the cell data;
s2: acquiring inventory transfer demand data based on an inventory transfer form, calling a formal library form and a temporary library form which are related to the inventory transfer form according to the inventory transfer demand data and cell data, and acquiring the position data of a to-be-transferred frozen storage tube in the formal library form or the temporary library form and the type and position of the temporary library form, and completing the transfer of the data in the formal library form and the temporary library form after the type and position of the formal library form or the temporary library form; when transferring data to a formal library form, quality inspection data needs to be acquired, and the transfer of the data in the temporary library form to the formal library form is completed after the quality inspection data is qualified.
A dynamic whole-flow cell inventory transfer system, comprising:
a retrieval module configured to receive cell data of the inventory transfer and retrieve an inventory transfer form after receiving the cell data;
the transfer module is configured to acquire inventory transfer demand data based on the inventory transfer form, call a formal library form and a temporary library form which are associated with the inventory transfer form according to the inventory transfer demand data and the cell data, and complete the transfer of the data in the formal library form and the temporary library form after acquiring the position data of a frozen tube to be transferred in the formal library form or the temporary library form and the type, the position or the position of the temporary library form; when transferring data to a formal library form, quality inspection data needs to be acquired, and the transfer of the data in the temporary library form to the formal library form is completed after the quality inspection data is qualified.
The beneficial effects of the invention are as follows:
the invention provides a new dynamic whole-flow cell inventory transfer method and a system, which can be used for quickly and accurately carrying out inventory transfer of cells based on comprehensive judgment of various factors, and the inventory transfer is not completed in a specific module, but is dynamically inserted in each link where the inventory transfer operation is likely to occur.
Drawings
FIG. 1 is a flow chart of a dynamic whole-flow cell inventory transfer method according to one embodiment of the invention;
FIG. 2 is a flow chart of a dynamic whole-flow cell inventory transfer method according to the present invention;
FIG. 3 is a flow chart of step S2 of the present invention;
FIG. 4 is a flowchart of steps S231-S233 of the present invention;
FIG. 5 is a flowchart of steps S234-S235 of the present invention;
FIG. 6 is a block diagram of a dynamic whole-flow cell inventory transfer system according to the present invention;
fig. 7 is a block diagram of a transfer module according to the present invention.
Detailed Description
The invention will be described in further detail with reference to the accompanying drawings and the following examples.
At least one embodiment provides a dynamic whole-process cell inventory transfer method, as shown in fig. 1, comprising the steps of:
s1: receiving cell data of stock transfer, and calling a stock transfer form after receiving the cell data;
s2: acquiring inventory transfer demand data based on an inventory transfer form, calling a formal library form and a temporary library form which are related to the inventory transfer form according to the inventory transfer demand data and cell data, and acquiring the position data of a to-be-transferred frozen storage tube in the formal library form or the temporary library form and the type and position of the temporary library form, and completing the transfer of the data in the formal library form and the temporary library form after the type and position of the formal library form or the temporary library form; when transferring data to a formal library form, quality inspection data needs to be acquired, and the transfer of the data in the temporary library form to the formal library form is completed after the quality inspection data is qualified.
The invention provides a new dynamic whole-flow cell inventory transfer method, which can be used for rapidly and accurately carrying out inventory transfer of cells based on comprehensive judgment of various factors, and the occurrence of the inventory transfer is not completed in a specific module, but is dynamically inserted in each link where the inventory transfer operation is likely to occur.
As shown in fig. 2, the specific steps of the method are as follows:
(1) Acquiring inventory transfer requirements, jumping to an inventory transfer page, and recording inventory transfer requirement sources on the page by experimenters;
(2) Judging a transfer type according to the stock transfer demand source, wherein the transfer type comprises production, loading seed cells into a formal warehouse and production of the seed cells in the formal warehouse;
(3) When the transfer type is production-seed cells enter a formal warehouse, acquiring the position of a cryopreservation tube to be transferred of a temporary warehouse, judging whether quality inspection is finished, if not, feeding back information, ending the flow, and if so, judging whether the quality inspection is qualified;
(4) When the quality inspection is qualified, selecting the position of a formal library, and transferring to a warehouse;
(5) And (3) obtaining the position of a cryopreservation tube to be transferred in the formal library when the transfer type is seed cell formal library-production, selecting the temporary library type/position, and transferring and warehousing.
In the process of culturing cells, the cells have the requirements of storing different generations into different entity libraries (liquid nitrogen tanks, ultralow temperature refrigerators and 4 ℃ refrigerators). That is, when cells exist in various forms (generations) during the culture, such as seed cells, working cells, etc. If the seed cells need to be stored for a long time, the seed cells need to be temporarily stored in an ultralow temperature refrigerator before being formally stored in liquid nitrogen with the storage environment of-196 ℃ for waiting for the relevant quality inspection results. This transfer between different physical environments is called cell inventory transfer. The cell stock transfer is greatly different from the stock transfer in the past warehouse system, and the main difference is that the physical environment or the stock transfer is determined by specific experimenters according to factors such as the actual cell culture state, culture stage and the like to be comprehensively considered. Thus, the occurrence of inventory transfers is not accomplished in a particular module, but rather is dynamically interspersed with each link where inventory transfer operations are likely to occur.
As shown in fig. 3, in at least one embodiment, the transferring method of step S2 may be implemented by using a neural network, and the specific steps are as follows:
s21: collecting inventory transfer data of cells and historical inventory transfer demand data corresponding to the inventory transfer data, and constructing 2 or more training sets;
s22: performing dimension reduction on each training set by at least one dimension reduction algorithm, wherein the dimension reduction algorithm comprises but is not limited to PCA theory; learning the training set after dimension reduction through at least one learning algorithm, and sequentially establishing 2 or more inventory transfer models, wherein the learning algorithm comprises but is not limited to an artificial intelligence deep learning technology;
s23: inputting actual stock transfer demand data into a corresponding stock transfer model, outputting preliminary stock transfer data, and performing step S24 after the data completely pass verification; after the partial verification is passed, the actual stock transfer demand data and the output partial preliminary stock transfer data are sequentially input into a next stock transfer model, further stock transfer data are output, and after the partial verification is completely passed, step S24 is performed; when some parts of the inventory transfer data are not verified, the steps of sequentially inputting the actual inventory transfer demand data and the part of the inventory transfer data output before into the inventory transfer model are circulated until all the inventory transfer data are output and the step S24 is performed;
s24: and transferring the data in the to-be-transferred library form to the determined library form through a data transfer tool.
In the embodiment, after the dimensions of inventory transfer data of cells and corresponding historical inventory transfer demand data are reduced, intelligent learning is performed to sequentially establish 2 or more inventory transfer models, actual inventory transfer demand data are sequentially input into each inventory transfer model, inventory transfer data are output, and inventory data are transferred after verification is passed; the design can automatically and sequentially output all inventory transfer data so as to achieve the purpose of quickly, accurately and stably realizing data transfer.
The inventory transfer data in this embodiment includes a to-be-transferred library form, a position of a freezing tube in the to-be-transferred library form, a determination library form, and a position of a freezing tube to be transferred in the determination library form, where the to-be-transferred library form is a formal library form or a temporary library form, and the determination library form is a temporary library form or a formal library form; the stock transfer demand data comprises one or more of cell actual culture state data, culture stage data, quality inspection qualified data and serial numbers of the cryopreservation tubes to be transferred.
As shown in fig. 4, in order to further explain the method for implementing data transfer through the neural network, step S23 in this embodiment includes the following steps:
s231: inputting actual inventory transfer demand data into a first-order inventory transfer model, outputting a to-be-transferred inventory form and a confirmed inventory form, judging whether inventory transfer can be completed according to the to-be-transferred inventory form and the confirmed inventory form, if so, performing step S24, otherwise, performing step S232;
s232: outputting the actual stock transfer demand data and the to-be-transferred stock form to a second-order stock transfer model, outputting the position of the to-be-transferred freezing pipe in the to-be-transferred stock form, judging whether the stock transfer can be completed according to the to-be-transferred stock form, determining the stock form and the position data of the to-be-transferred freezing pipe in the to-be-transferred stock form, if so, performing step S24, otherwise, performing step S233;
s233: outputting the actual stock transfer demand data and the determined stock form to a third-order stock transfer model, outputting the position of the frozen storage tube to be transferred in the determined stock form, judging whether the stock transfer can be completed according to the to-be-transferred stock form, the determined stock form, the position of the frozen storage tube to be transferred in the to-be-transferred stock form and the position data of the frozen storage tube to be transferred in the to-be-transferred stock form, if so, performing step S24, otherwise, sending a prompt that the data has error transfer failure to a user.
As shown in fig. 5, step S23 in this embodiment further includes the following steps:
s234: judging whether the library form is a temporary library form or a formal library form, if the library form is the temporary library form, performing step S233, and if the library form is the formal library form, performing step S235;
s235: outputting the actual stock transfer demand data to a fourth-order stock transfer model when the position of the freezing storage pipe to be transferred in the stock form is determined and judging that the stock transfer cannot be completed, outputting the stock transfer instruction, judging whether the stock transfer can be completed according to the stock form to be transferred, the position of the freezing storage pipe to be transferred in the stock form, the position data of the freezing storage pipe to be transferred in the stock form and the stock transfer instruction, if yes, performing step S24, otherwise, sending a prompt that the data has error transfer failure to a user.
In the embodiment, the inventory transfer demand data are sequentially input into each inventory transfer model, so that the data identification is more rapid and accurate, and the error of the data in which step can be rapidly found out; when transferring data from the temporary storage warehouse to the formal warehouse, the quality of the cells needs to be checked, and the cells can be stored in the formal warehouse only after the quality check is finished and the quality check is qualified, so that the quality of the cells stored in the formal warehouse can be ensured.
In this embodiment, the inventory transfer instruction in step S235 is output based on the quality inspection qualified data, where the inventory transfer instruction includes an inventory transfer when the quality inspection is qualified and an inventory non-transfer when the quality inspection is not qualified or the quality inspection is not qualified.
In this embodiment, the single end of the formal library table creates a formal end external table, the single end of the temporary library table creates a temporary end external table, the formal end external table is associated with the number of the cryopreservation tube in the formal library table, the temporary end external table is associated with the number of the cryopreservation tube in the temporary library table, the inventory transfer model in the second order matches with the external table of the corresponding library table to be transferred through the number of the cryopreservation tube to be transferred, and the position of the cryopreservation tube to be transferred in the corresponding library table to be transferred is determined through the external table.
At least one embodiment provides a dynamic whole-process cell inventory transfer system, as shown in FIG. 6, comprising the following:
a retrieving module 10 configured to receive cell data of inventory transfers and retrieve an inventory transfer form after receiving the cell data;
a transfer module 20 configured to acquire inventory transfer demand data based on an inventory transfer form, retrieve a formal library form and a temporary library form associated with the inventory transfer form according to the inventory transfer demand data and the cell data, and complete transfer of data in the formal library form and the temporary library form after acquiring position data of a frozen tube to be transferred in the formal library form or the temporary library form and a type, a position or a position of the temporary library form; when transferring data to a formal library form, quality inspection data needs to be acquired, and the transfer of the data in the temporary library form to the formal library form is completed after the quality inspection data is qualified.
The invention provides a new dynamic whole-flow cell inventory transfer system, which can be used for rapidly and accurately carrying out inventory transfer of cells based on comprehensive judgment of various factors, and the occurrence of the inventory transfer is not completed in a specific module, but is dynamically inserted in each link where the inventory transfer operation is likely to occur.
As shown in fig. 7, in at least one embodiment, the transfer method in the transfer module 20 may be implemented by using a neural network, which is specifically as follows:
a data collection unit 201 configured to collect inventory transfer data of cells and corresponding historical inventory transfer demand data thereof, and construct 2 or more training sets;
a model building unit 202 configured to dimension-reduce each of the training sets by at least one dimension-reduction algorithm; learning the training set after dimension reduction by at least one learning algorithm, and sequentially establishing 2 or more inventory transfer models;
the data processing unit 203 is configured to input actual inventory transfer requirement data into a corresponding inventory transfer model, output preliminary inventory transfer data, and send a data transfer instruction to the data transfer unit 204 after the data is completely verified; after the partial verification is passed, the actual stock transfer demand data and the output partial preliminary stock transfer data are sequentially input into a next stock transfer model, further stock transfer data are output, and after the partial verification is completely passed, step S24 is performed; when some parts of the inventory transfer data are not verified, the steps of sequentially inputting the actual inventory transfer demand data and the part of the inventory transfer data output before into the inventory transfer model are circulated until all the inventory transfer data are output and a data transfer instruction is sent to the data transfer unit 204;
a data transfer unit 204 configured to transfer data in the library form to be transferred into the determination library form by a data transfer tool.
The inventory transfer data in the data collection unit 201 in this embodiment includes a to-be-transferred library form, a position of a freezing tube in the to-be-transferred library form, a determination library form, and a position of a freezing tube to be transferred in the determination library form, where the to-be-transferred library form is a formal library form or a temporary library form, and the determination library form is a temporary library form or a formal library form; the actual stock transfer requirement data comprises one or more of cell actual culture state data, culture stage data, quality inspection qualified data and serial numbers of the cryopreservation tubes to be transferred.
In the embodiment, after the dimensions of inventory transfer data of cells and corresponding historical inventory transfer demand data are reduced, intelligent learning is performed to sequentially establish 2 or more inventory transfer models, actual inventory transfer demand data are sequentially input into each inventory transfer model, inventory transfer data are output, and inventory data are transferred after verification is passed; the design can automatically and sequentially output all inventory transfer data so as to achieve the purpose of quickly, accurately and stably realizing data transfer.
The above examples are merely illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solution of the present invention should fall within the scope of protection defined by the claims of the present invention without departing from the spirit of the present invention.
Claims (8)
1. A dynamic whole-flow cell inventory transfer method, comprising the steps of:
s1: receiving cell data of stock transfer, and calling a stock transfer form after receiving the cell data;
s2: acquiring inventory transfer demand data based on an inventory transfer form, calling a formal library form and a temporary library form which are associated with the inventory transfer form according to the inventory transfer demand data and cell data, and completing transfer of data in the formal library form and the temporary library form after acquiring the position data of a to-be-transferred frozen storage tube in the formal library form or the temporary library form and the type, the position or the position of the formal library form; when transferring data to a formal library form, quality inspection data is required to be acquired, and transferring the data in the temporary library form to the formal library form is completed after the quality inspection data is qualified;
in step S2, the data transfer is completed according to the following steps:
s21: collecting inventory transfer data of cells and historical inventory transfer demand data corresponding to the inventory transfer data, and constructing more than 2 training sets;
s22: performing dimension reduction on each training set through at least one dimension reduction algorithm; learning the training set after dimension reduction by at least one learning algorithm, and sequentially establishing more than 2 inventory transfer models;
s23: inputting actual stock transfer demand data into a corresponding stock transfer model, outputting preliminary stock transfer data, and performing step S24 after the data completely pass verification; after the partial verification is passed, the actual stock transfer demand data and the output partial preliminary stock transfer data are sequentially input into a next stock transfer model, further stock transfer data are output, and after the partial verification is completely passed, step S24 is performed; when some parts of the inventory transfer data are not verified, the steps of sequentially inputting the actual inventory transfer demand data and the part of the inventory transfer data output before into the inventory transfer model are circulated until all the inventory transfer data are output and the step S24 is performed;
s24: and transferring the data in the to-be-transferred library form to the determined library form through a data transfer tool.
2. The method for dynamically transferring the inventory of the whole flow cell according to claim 1, wherein the inventory transfer data comprises a to-be-transferred inventory form, a position of a freezing tube in the to-be-transferred inventory form, a determination inventory form and a position of the freezing tube to be transferred in the inventory form, the to-be-transferred inventory form is a formal inventory form or a temporary inventory form, and the determination inventory form is a temporary inventory form or a formal inventory form; the actual stock transfer requirement data comprises one or more of cell actual culture state data, culture stage data, quality inspection qualified data and serial numbers of the cryopreservation tubes to be transferred.
3. The method of claim 2, wherein step S23 comprises the steps of:
s231: inputting actual inventory transfer demand data into a first-order inventory transfer model, outputting a to-be-transferred inventory form and a confirmed inventory form, judging whether inventory transfer can be completed according to the to-be-transferred inventory form and the confirmed inventory form, if so, performing step S24, otherwise, performing step S232;
s232: outputting the actual stock transfer demand data and the to-be-transferred stock form to a second-order stock transfer model, outputting the position of the to-be-transferred freezing pipe in the to-be-transferred stock form, judging whether the stock transfer can be completed according to the to-be-transferred stock form, determining the stock form and the position data of the to-be-transferred freezing pipe in the to-be-transferred stock form, if so, performing step S24, otherwise, performing step S233;
s233: outputting the actual stock transfer demand data and the determined stock form to a third-order stock transfer model, outputting the position of the frozen storage tube to be transferred in the determined stock form, judging whether the stock transfer can be completed according to the to-be-transferred stock form, the determined stock form, the position of the frozen storage tube to be transferred in the to-be-transferred stock form and the position data of the frozen storage tube to be transferred in the to-be-transferred stock form, if so, performing step S24, otherwise, sending a prompt that the data has error transfer failure to a user.
4. The method of claim 3, wherein step S23 further comprises the steps of:
s234: judging whether the library form is a temporary library form or a formal library form, if the library form is the temporary library form, performing step S233, and if the library form is the formal library form, performing step S235;
s235: outputting the actual stock transfer demand data to a fourth-order stock transfer model when the position of the freezing storage pipe to be transferred in the stock form is determined and judging that the stock transfer cannot be completed, outputting the stock transfer instruction, judging whether the stock transfer can be completed according to the stock form to be transferred, the position of the freezing storage pipe to be transferred in the stock form, the position data of the freezing storage pipe to be transferred in the stock form and the stock transfer instruction, if yes, performing step S24, otherwise, sending a prompt that the data has error transfer failure to a user.
5. The method according to claim 4, wherein the inventory transfer instruction in step S235 is output based on quality inspection qualified data, and the inventory transfer instruction includes an inventory transfer when quality inspection is qualified and an inventory not transfer when quality inspection is not qualified or quality inspection is not qualified.
6. The method according to claim 2, wherein the single end of the formal library table creates a formal end external table, the single end of the temporary library table creates a temporary end external table, the formal end external table is associated with the number of the cryopreservation tube in the formal library table, the temporary end external table is associated with the number of the cryopreservation tube in the temporary library table, the inventory transfer model in the second order is matched with the external table of the corresponding library table to be transferred through the number of the cryopreservation tube to be transferred, and the position of the cryopreservation tube to be transferred in the corresponding library table to be transferred is determined through the external table.
7. A dynamic whole-flow cell inventory transfer system, comprising: a retrieval module (10) configured to receive the cell data of the inventory transfer and retrieve the inventory transfer form after receiving the cell data;
a transfer module (20) configured to acquire inventory transfer demand data based on an inventory transfer form, retrieve a formal library form and a temporary library form associated with the inventory transfer form according to the inventory transfer demand data and the cell data, and complete transfer of the formal library form and the temporary library form after acquiring position data of a frozen tube to be transferred in the formal library form or the temporary library form and a type, a position or a position of the formal library form; when transferring data to a formal library form, quality inspection data is required to be acquired, and transferring the data in the temporary library form to the formal library form is completed after the quality inspection data is qualified;
the transfer module (20) performs data transfer according to the following steps:
the data acquisition unit (201) is configured to acquire inventory transfer data of cells and corresponding historical inventory transfer demand data thereof, and construct more than 2 training sets;
a model construction unit (202) configured to dimension-reduce each of the training sets by at least one dimension-reduction algorithm; learning the training set after dimension reduction by at least one learning algorithm, and sequentially establishing more than 2 inventory transfer models;
a data processing unit (203) configured to input actual inventory transfer demand data into a corresponding inventory transfer model, output preliminary inventory transfer data, and after passing the verification completely, send a data transfer instruction to a data transfer unit (204); when the partial verification is passed, the actual stock transfer demand data and the output partial preliminary stock transfer data are sequentially input into a next stock transfer model, further stock transfer data are output, and after the partial verification is passed, a data transfer instruction is sent to a data transfer unit (204); when some parts of the inventory transfer data are not verified, the steps of sequentially inputting the actual inventory transfer demand data and the part of the inventory transfer data output before into the inventory transfer model are circulated until all inventory transfer data are output and a data transfer instruction is sent to a data transfer unit (204);
a data transfer unit (204) configured to transfer data in the library form to be transferred into the determined library form by means of a data transfer tool.
8. The dynamic whole-flow cell inventory transfer system according to claim 7, wherein the inventory transfer data in the data collection unit (201) includes a to-be-transferred library form, a position of a cryopreservation tube in the to-be-transferred library form, a determination library form, and a position of a cryopreservation tube to be transferred in the library form, the to-be-transferred library form is a formal library form or a temporary library form, and the determination library form is a temporary library form or a formal library form; the actual stock transfer requirement data comprises one or more of cell actual culture state data, culture stage data, quality inspection qualified data and serial numbers of the cryopreservation tubes to be transferred.
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| US20030101107A1 (en) * | 2001-11-29 | 2003-05-29 | Rishi Agarwal | Inventory management system and method |
| US8949032B2 (en) * | 2002-03-29 | 2015-02-03 | Genomatica, Inc. | Multicellular metabolic models and methods |
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| CN109409790A (en) * | 2018-09-06 | 2019-03-01 | 航天信息股份有限公司 | A kind of negative outbound management control method of inventory and system |
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