CN111227881A

CN111227881A - Dissection device of fruit fly larva wing bud

Info

Publication number: CN111227881A
Application number: CN202010181670.3A
Authority: CN
Inventors: 张徐波; 董玮; 张建珍
Original assignee: Shanxi University
Current assignee: Shanxi University
Priority date: 2020-03-16
Filing date: 2020-03-16
Publication date: 2020-06-05
Anticipated expiration: 2040-03-16
Also published as: CN111227881B

Abstract

The invention provides a device for dissecting wing buds of drosophila larvae, belongs to the field of insect dissection, and aims to solve the problems of time and labor waste and low dissection efficiency in the conventional mode. Including outer bucket, the inside of outer bucket is rather than coaxial interior bucket that is equipped with, interior bucket surface is equipped with a plurality of hole, every hole correspondence is equipped with a thimble, thimble one end stretches into in the hole, the thimble other end is connected with the top sheet, a spring is established to the cover on the thimble, spring one end and the interior wall connection of interior bucket, the spring other end is connected with the top sheet, the one end that is close to outer bucket in the hole is equipped with the fruit bat stopper, interior bucket top is equipped with columniform blade, the blade surface is equipped with the round hole with the hole one-to-one correspondence, blade inside is equipped with rather than coaxial top sheet pusher, blade and top sheet pusher all are connected with outer bucket top through the fixed bolster, the bottom of outer bucket is equipped with the outer bucket temperature control system who.

Description

Dissection device of fruit fly larva wing bud

Technical Field

The invention relates to the technical field of insect dissection, in particular to a device for dissecting winged buds of drosophila larvae.

Background

Drosophila is a classical model organism. The drosophila larva wing bud is a research object, and a good research model is provided for organ development. The winged bud of drosophila larvae is relatively simple in structure, and is located approximately 1/3 away from the head of the larvae. The research of wing buds usually needs to dissect the wing buds first and determine the expression condition of genes through immunohistochemical staining.

The current method for dissecting the winged bud of the drosophila larvae comprises the following steps: first, the 3 rd larva of fruit fly filial generation is cut into two sections by using tweezers, and the latter half-section larva body is discarded. Then, the distal cuticle of the first half larva was grasped with a pair of tweezers, and the head of the larva was pushed against another pair of tweezers to turn out the internal organs of the first half larva, thereby exposing the winged bud of the drosophila larva. Then, the dissected tissue was fixed with formaldehyde fixing solution, and the subsequent immunohistochemical procedure was performed. However, because the number of the winged buds of the drosophila larvae is large in the research process, the dissection mode is time-consuming, labor-consuming and low in dissection efficiency.

Disclosure of Invention

The invention provides a fruit fly larva wing bud dissection device, which aims to solve the technical problems of time and labor waste and low dissection efficiency in the current fruit fly larva wing bud dissection mode.

In order to solve the technical problems, the invention adopts the technical scheme that:

an dissecting device for the winged bud of fruit fly larva is composed of an external drum, an internal drum coaxial with said external drum and with several holes through its wall and arranged in array mode, a top needle with one end extending into said hole and another end connected to top plate, a spring on said top needle and with one end fixed to the inner wall of internal drum, a fruit fly limiter near the external drum, a cylindrical blade with bottom blade and several holes on its surface, a top plate pusher coaxial with said blade and in the shape of bullet, and a top plate pusher connected to the top of external drum via fixing frame, the bottom of outer bucket is provided with outer bucket temperature control system who is connected with outer bucket, the bottom of outer bucket is connected with the base, the bottom of interior bucket is connected with interior bucket temperature control system, interior bucket temperature control system sets up in the base, still be provided with the motor in the base, the hub connection of motor has the circular shape rolling disc, the axis of rotation is installed at the edge of rolling disc, install annular impeller in the bottom clearance between outer bucket and the interior bucket, the axis of rotation is connected with the impeller, motor, outer bucket temperature control system and interior bucket temperature control system all are connected with the power.

Optionally, one end of the thimble is bullet-shaped.

Optionally, two sides of the top of the blade are provided with hand-push handles, and two sides of the top sheet pusher are provided with handles.

Optionally, a drain pipe is arranged on one side of the bottom of the outer barrel, and a water stop clamp is arranged on a water outlet of the drain pipe in a matched manner.

The invention has the beneficial effects that:

through setting up outer bucket, interior bucket, blade, thimble, top piece and top piece pusher 9 isotructures, provide one kind and can once only dissect the device to the wing bud of many fruit fly larvae, dissect the wing bud of fruit fly larva through the device, labour saving and time saving not only can improve the efficiency of dissecting moreover.

Drawings

Fig. 1 is a schematic structural view of the present invention.

Fig. 2 is a top view of fig. 1.

Fig. 3 is a partially enlarged view of a portion a in fig. 1.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and examples.

As shown in fig. 1 to 3, the device for dissecting drosophila larvae wing buds in the present embodiment comprises an outer barrel 1, an inner barrel 2 coaxially arranged inside the outer barrel 1 and the outer barrel 1, a plurality of holes 3 penetrating through the barrel wall of the inner barrel 2 arranged on the surface of the inner barrel 2, a plurality of holes 3 arranged in an array form, each hole 3 being correspondingly provided with a thimble 4, one end of the thimble 4 extending into the hole 3, the other end of the thimble 4 in the same row being connected with a top plate 5, a spring 6 sleeved on one thimble 4, one end of the spring 6 being fixedly connected with the inner wall of the inner barrel 2 around the hole 3, the other end of the spring 6 being fixedly connected with the top plate 5, a drosophila fruit stopper 11 arranged at the end of the hole 3 close to the outer barrel 1, a cylindrical blade 7 arranged at the top end of the inner barrel 2, a blade edge at the bottom of the blade 7, a plurality of round, the blade 7 is internally provided with a top sheet pusher 9 coaxial with the blade 7, the top sheet pusher 9 is bullet-shaped, the blade 7 and the top sheet pusher 9 are both connected with the top of the outer barrel 1 through a fixing support 10, the bottom of the outer barrel 1 is provided with an outer barrel temperature control system 12 connected with the outer barrel 1, the bottom of the outer barrel 1 is connected with a base 13, the bottom of the inner barrel 2 is connected with an inner barrel temperature control system 14, the inner barrel temperature control system 14 is arranged in the base 13, the base 13 is also provided with a motor 22, the shaft of the motor 22 is connected with a circular rotating disc 15, the edge of the rotating disc 15 is provided with a rotating shaft 16, an annular impeller 17 is arranged in a bottom gap between the outer barrel 1 and the inner barrel 2, the rotating shaft 16 is connected with the impeller 17, and the motor 22, the outer barrel temperature.

Wherein, the outer barrel 1 and the inner barrel 2 are made of metal materials with better heat conductivity.

Optionally, one end of the thimble 4 is bullet-shaped, so that one end of the thimble 4 is relatively blunt, so as to eject the fruit fly larvae.

Optionally, the blade 7 is provided with a handle 18 on both sides of the top, and the top pusher 9 is provided with a handle 19 on both sides of the top.

Optionally, a drain pipe 20 is disposed at one side of the bottom of the outer tub 1, and a water stop clamp 21 is disposed at a water outlet of the drain pipe 20 in a matching manner.

Wherein, the wires of the motor 22, the outer barrel temperature control system 12 and the inner barrel temperature control system 14 connected with the power supply are respectively provided with respective control switches.

When the fruit fly larva cleaning device is used, water is added into a gap between the outer barrel 1 and the inner barrel 2, and then fruit fly larvae are placed into the water. Then, the power is turned on, the outer tub temperature control system 12 controls the outer tub 1 to raise the temperature to 37-42 ℃ in a short time and maintains the temperature, and the inner tub temperature control system 14 controls the inner tub 2 to raise the temperature to 20-23 ℃ in a short time and maintains the temperature. Because the fruit fly larvae have the characteristic of avoiding high temperature in water, the fruit fly larvae can hide in a low-temperature place, and at the moment, the fruit fly larvae can drill into the inner barrel 2 through the holes 3. When the fruit fly larvae drill into half of the holes 3, the fruit fly larvae are blocked by the ejector pins 4 to be prevented from further drilling forwards, and half of the bodies of the fruit fly larvae are positioned outside the holes 3. Because a fruit fly stopper 11 is arranged in each hole 3, the fruit fly larvae can not back up at the moment. When more fruit fly larvae are drilled into the holes 3, the blade 7 is pushed downwards until the blade edge contacts the inner bottom of the inner barrel 2, the blade 7 is used for blocking and cutting off the plurality of fruit fly larvae, and at the moment, the round hole 8 is overlapped with the holes 3. Next, the top plate pusher 9 is pushed downward, the top plate 5 is pushed by the top plate pusher 9 to move the top plate 5 toward the outer tub 1, so that the end of the thimble 4 pushes half of the larvae out of the hole 3, and the skin at the end of the cut of the drosophila larvae is blocked by the drosophila stopper 11, so that the skin of the larvae is turned over, and the viscera are turned over to expose organ buds such as wing buds. Then, the motor 22 is turned on to rotate the motor 22 at a low speed, the motor 22 rotates to drive the rotary disc 15 to rotate, the rotary disc 15 rotates to drive the rotary shaft 16 and the impeller 17 to rotate, and the impeller 17 rotates to enable the water between the outer barrel 1 and the inner barrel 2 to start shaking so as to achieve the effect of cleaning the dissected impurities such as fat bodies and the like of the drosophila melanogaster. Then, the dissected fruit flies on each thimble 4 are picked up by tweezers. After the above processes are completed, the power supply is turned off, the blade 7 and the top sheet pusher 9 are moved upwards and fixed through the fixing support 10, the spring 6 is reset at the moment, one end of the thimble 4 is positioned in the middle of the hole 3, and then the next poking dissection can be carried out. Finally, the water stop clamp 21 on the drain pipe 20 is opened to drain the waste liquid.

Optionally, the outer wall of the inner barrel 2 is provided with a thermal insulation material layer to ensure that the temperature of the inner barrel 2 can be kept at 20-23 ℃, and the temperature of the inner barrel 2 is prevented from being influenced by the temperature of water.

It will be understood that the above embodiments are merely exemplary embodiments taken to illustrate the principles of the present invention, which is not limited thereto. It will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the spirit and substance of the invention, and these modifications and improvements are also considered to be within the scope of the invention.

Claims

1. An dissecting device for drosophila larva wing buds is characterized by comprising an outer barrel (1), an inner barrel (2) is coaxially arranged inside the outer barrel (1) and the outer barrel (1), a plurality of holes (3) penetrating through the barrel wall of the inner barrel (2) are formed in the surface of the inner barrel, the holes (3) are arranged in an array mode, each hole (3) is correspondingly provided with a thimble (4), one end of each thimble (4) extends into each hole (3), the other end of each thimble (4) positioned in the same row is connected with a top plate (5), a spring (6) is sleeved on each thimble (4), one end of each spring (6) is fixedly connected with the inner wall of the inner barrel (2) around each hole (3), the other end of each spring (6) is fixedly connected with the top plate (5), a drosophila stopper (11) is arranged at one end, close to the outer barrel (1), of each hole (3), a cylindrical blade (7) is arranged at the top end, the bottom of the blade (7) is a cutting edge, the surface of the blade (7) is provided with a plurality of round holes (8) which are in one-to-one correspondence with the holes (3), a top plate pusher (9) which is coaxial with the blade (7) is arranged inside the blade (7), the top plate pusher (9) is bullet-shaped, the blade (7) and the top plate pusher (9) are both connected with the top of the outer barrel (1) through a fixed support (10), the bottom of the outer barrel (1) is provided with an outer barrel temperature control system (12) which is connected with the outer barrel (1), the bottom of the outer barrel (1) is connected with a base (13), the bottom of the inner barrel (2) is connected with an inner barrel temperature control system (14), the inner barrel temperature control system (14) is arranged in the base (13), a motor (22) is also arranged in the base (13), a round rotating disc (15) is connected with a shaft of the motor (22), and a rotating, an annular impeller (17) is arranged in a bottom gap between the outer barrel (1) and the inner barrel (2), a rotating shaft (16) is connected with the impeller (17), and the motor (22), the outer barrel temperature control system (12) and the inner barrel temperature control system (14) are all connected with a power supply.

2. The device for dissecting fruit fly larva winged buds according to claim 1, wherein one end of the thimble (4) is bullet-shaped.

3. The device for dissecting fruit fly larva wing buds according to claim 1, wherein the blade (7) is provided with hand push handles (18) on both sides of the top, and the top sheet pusher (9) is provided with handles (19) on both sides of the top.

4. The fruit fly larva wing bud dissection device according to claim 1, wherein a drain pipe (20) is arranged on one side of the bottom of the outer barrel (1), and a water stop clamp (21) is arranged on a water outlet of the drain pipe (20) in a matched manner.