CN111226930A - Locust migratory locust nymph attractant and bait and application thereof - Google Patents
Locust migratory locust nymph attractant and bait and application thereof Download PDFInfo
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- CN111226930A CN111226930A CN202010182761.9A CN202010182761A CN111226930A CN 111226930 A CN111226930 A CN 111226930A CN 202010182761 A CN202010182761 A CN 202010182761A CN 111226930 A CN111226930 A CN 111226930A
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- attractant
- locust
- nymph
- locusta migratoria
- heptanone
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
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- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a locusta migratoria nymphs attractant as well as a lure and application thereof, wherein the locusta migratoria nymphs attractant consists of 2-heptanone and paraffin oil; the luring core of the locusta migratoria nymph attractant is prepared by the following preparation method: uniformly mixing the paraffin oil and the 2-heptanone to obtain a locusta migratoria nymph attractant; and soaking the rubber strip in a locusta migratoria nymph attractant for 1-2 hours, drying in the shade, and sealing with plastic to obtain the lure. The attractant is a preparation capable of regulating the behavior of migratory locusts, and can be used for controlling the behavior of migratory locusts, and preventing, controlling and monitoring the population quantity dynamics of migratory locusts. Because the 2-heptanone is a migratory locust metabolite, the pesticide has no toxic action on the environment, human beings, livestock and poultry, is safe to natural enemies and belongs to natural pesticides.
Description
Technical Field
The invention relates to the technical field of pest control, in particular to a locust migratory nymph attractant separated from volatile matters of locust migratory, a core lure and application thereof.
Background
Locust is a great pest which seriously threatens the production of agriculture and animal husbandry in China. Migratory locusts are the most important species causing devastating disasters in agriculture. The existing method for preventing and controlling the pests is mainly chemical pesticide control, and although locust can be effectively controlled by using a large amount of chemical pesticide, adverse effects such as environmental pollution, overproof chemical pesticide residue of agricultural products, increased locust drug resistance, increased control cost, severe cases frequently causing poisoning of people and livestock and the like can be brought. Therefore, the adoption of a control method which can replace chemical pesticides so as to achieve the aim of sustainable development is a very urgent task at present. Insect behavior modifiers, also known as pheromones, are generally compounds obtained from natural environments that modulate insect behavior and are therefore degradable in nature. And the insects are extremely sensitive to pheromones, so the use amount is low generally, the environment and agricultural products are not easy to be polluted, the pests are not easy to generate drug resistance, and the pesticide is nontoxic to people and livestock. Is widely considered to have wide prospect in the field of pest control in the world. Researches have been made on pheromone behavior regulators of desert locusts and african migratory locusts. Since migratory locust is not the same species as desert locust and is not a uniform subspecies as African migratory locust, here we describe that our discovered migratory locust pheromone is significantly different from desert locust and African migratory locust. Migratory locusts are major pests seriously threatening the production of agriculture and animal husbandry in China, the existing method for preventing and controlling the pests is mainly chemical pesticide, and no report is provided for the attractant of the pests.
Disclosure of Invention
The invention provides a locust locusta nymph attractant, a core lure and application thereof, which aim to solve the problem that the existing method for controlling locusts is mainly chemical pesticide.
In order to solve the technical problem, the technical scheme of the invention is realized as follows:
a locusta migratoria nymphs attractant is composed of 2-heptanone and paraffin oil.
Wherein, preferably, the volume ratio of the paraffin oil to the 2-heptanone is 1 ml: (0.1-1) μ L.
A core attractant of a locusta migratoria nymph attractant is prepared by the following preparation method:
(1) uniformly mixing the paraffin oil and the 2-heptanone to obtain a locusta migratoria nymph attractant;
(2) soaking the rubber strip in locusta migratoria nymph attractant for 1-2 hr, drying in the shade, and sealing with plastic to obtain the lure.
Wherein, preferably, the area of the lure is 20X20 cm.
Wherein, the soaking temperature in the step (2) is preferably 20-30 ℃.
The locust migratory nymph attractant and the application of the locust migratory nymph attractant in the field of locust control are used for attracting the locust migratory nymphs to cause a tropism reaction of the locust migratory nymphs and can control the behavior of the locust migratory, so that the locust migratory nymphs are controlled, centralized trapping and killing are performed, and the population quantity and the locust plague trend of the locust migratory are dynamically monitored.
The invention has the beneficial effects that:
the locusta migratoria can accept a plurality of chemical substances and generate behavior reaction, and the attractant disclosed by the invention is a preparation capable of regulating the behavior of the locusta migratoria, and can be used for controlling the behavior of the locusta migratoria, preventing and monitoring the population quantity dynamic of the locusta migratoria. Because the 2-heptanone is a migratory locust metabolite, the pesticide has no toxic action on the environment, human beings, livestock and poultry, is safe to natural enemies and belongs to natural pesticides.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 12 is a graph showing the luring effect of heptanone on nymphs of locusta migratoria at 4 th instar;
FIG. 2 is a graph showing the behavior response (luring index) trend of locusta migratoria nymphs at four ages to different concentrations of 2 heptanone;
FIG. 3 is a graph showing the behavior response trend of five-instar nosema locustae nymphs to 2-heptanone;
FIG. 4 is a trend graph of behavioral response (luring index) of five-instar locust nymphs to different concentrations of 2-heptanone.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A locusta migratoria nymphs attractant is composed of 2-heptanone and paraffin oil. Various paraffin oils are suitable for the attractant, such as those available from Jiangsu Zheng Xue chemical Co.
Wherein, preferably, the volume ratio of the paraffin oil to the 2-heptanone is 1 ml: (0.1-1) μ L; more preferably 1 ml: 0.5. mu.L.
A core attractant of a locusta migratoria nymph attractant is prepared by the following preparation method:
(1) uniformly mixing the paraffin oil and the 2-heptanone to obtain a locusta migratoria nymph attractant;
(2) and soaking the rubber strip in a locusta migratoria nymph attractant for 1-2 hours, drying in the shade, and sealing with plastic to obtain the lure.
Wherein, preferably, the area of the lure is 20X20 cm.
Wherein, the soaking temperature in the step (2) is preferably 20-30 ℃, more preferably 25 DEG C
The locust migratory nymph attractant and the application of the locust migratory nymph attractant in the field of locust control are used for attracting the locust migratory nymphs to cause a tropism reaction of the locust migratory nymphs and can control the behavior of the locust migratory, so that the locust migratory nymphs are controlled, centralized trapping and killing are performed, and the population quantity and the locust plague trend of the locust migratory are dynamically monitored.
Example 1
The embodiment provides a locusta migratoria nymph attractant which consists of 2-heptanone and paraffin oil. Wherein the volume ratio of the paraffin oil to the 2-heptanone is 1 ml: 0.5. mu.L.
The embodiment also provides a lure adopting the locust locusta migratoria nymph attractant, which is prepared by the following preparation method:
(1) uniformly mixing the paraffin oil and the 2-heptanone to obtain a locusta migratoria nymph attractant;
(2) soaking the rubber strip in locusta migratoria nymph attractant for 1.5 h at 25 deg.C, drying in the shade, making into 20X20 cm luring core, and sealing with plastic.
Example 2
The embodiment provides a locusta migratoria nymph attractant which consists of 2-heptanone and paraffin oil. Wherein the volume ratio of the paraffin oil to the 2-heptanone is 1 ml: 0.1. mu.L.
The embodiment also provides a lure adopting the locust locusta migratoria nymph attractant, which is prepared by the following preparation method:
(1) uniformly mixing the paraffin oil and the 2-heptanone to obtain a locusta migratoria nymph attractant;
(2) soaking the rubber strip in locusta migratoria nymph attractant for 1 hour at the soaking temperature of 30 ℃, drying in the shade to prepare a 20X20 cm attracting core, and sealing with plastic.
Example 3
The embodiment provides a locusta migratoria nymph attractant which consists of 2-heptanone and paraffin oil. Wherein the volume ratio of the paraffin oil to the 2-heptanone is 1 ml: 1 μ L.
The embodiment also provides a lure adopting the locust locusta migratoria nymph attractant, which is prepared by the following preparation method:
(1) uniformly mixing the paraffin oil and the 2-heptanone to obtain a locusta migratoria nymph attractant;
(2) soaking the rubber strip in locusta migratoria nymph attractant for 2 hours at the soaking temperature of 20 ℃, drying in the shade to prepare a 20X20 cm attracting core, and sealing with plastic to obtain the locust attractant.
Application example 1
The mixture containing 2-heptanone 0.1-100 μ l per ml paraffin oil is used for measuring migratory locust tendency reaction by locust olfaction behavioral analyzer. The locusta migratoria nymphs are found to be very different from each other by 68 percent of the locusta migratoria nymphs and 32 percent of the locusta migratoria nymphs and the solvent only. But the adults have no apparent tropism.
The luring effect of 2-heptanone on locusta migratoria nymphs at 4 th instar is shown in fig. 1.
Behavioral responses (luring index) of locusta migratoria nymphs at four ages to different concentrations of 2 heptanone are shown in fig. 2.
As shown in FIGS. 1 and 2, the nymphs of four-instar migratory locusts were significantly different in the selection of 2-heptanone at concentrations of 0.1. mu.l/ml, 0.5. mu.l/ml, 1. mu.l/ml from the control (P values of 0.02, 0.0005, 0.002), while at concentrations of 10. mu.l/ml, 30. mu.l/ml, 50. mu.l/ml, 100. mu.l/ml, no significant difference in the selection of control (P values of 0.29, 0.35, 0.49, 0.62). Four-instar locust nymphs had significant chemotactic responses to 0.1-1. mu.l/ml 2-heptanone, with 0.5. mu.l/ml chemotaxis being most pronounced (attraction index 38%) and no pronounced chemotactic response to other concentrations of 2-heptanone. Characteristic behaviors of the locust on the compound having the attracting effect, such as a creeping feeler and a body turning when climbing to a stimulus source, a walking when stopping, an in-situ turning, and the like, are observed in the test.
Application example 2 attractant action on five-instar locusta migratoria nymphs
The behavioral response of five-instar locusta migratoria nymphs to 2-heptanone is shown in FIG. 3; behavioral responses (luring index) of five-instar nymphs to different concentrations of 2-heptanone.
The results of the assays shown in fig. 3 and 4 show that: five-instar locust nymphs had significant differences in selection between 2-heptanone at concentrations of 0.1. mu.l/ml, 0.5. mu.l/ml, 1. mu.l/ml and the control (P values of 0.04,0.006,0.02, respectively) and between 10. mu.l/ml, 30. mu.l/ml, 50. mu.l/ml, 100. mu.l/ml and the control (P values of 0.56,0.72,1,0.64, respectively). Five-instar locust nymphs had significant chemotactic responses to 0.5-1. mu.l/ml 2-heptanone, with 0.5. mu.l/ml chemotaxis being the most pronounced (attraction index 38%) and no chemotactic response to other concentrations of 2-heptanone. Characteristic behavior was found in the experiments. The 2-heptanone is proved to have the attraction effect on the five-instar locust nymphs within a certain concentration range.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A locusta migratoria nymphs attractant is characterized by being composed of 2-heptanone and paraffin oil.
2. The locust nymph attractant according to claim 1, wherein: the volume ratio of the paraffin oil to the 2-heptanone is 1 ml: (0.1-1) μ L.
3. A lure of the locust nymph attractant of claim 1 or 2, which is prepared by the following preparation method:
(1) uniformly mixing the paraffin oil and the 2-heptanone to obtain a locusta migratoria nymph attractant;
(2) and soaking the rubber strip in a locusta migratoria nymph attractant for 1-2 hours, drying in the shade, and sealing with plastic to obtain the lure.
4. A lure of the locust nymph attractant of claim 3, wherein: the area of the lure is 20X20 cm.
5. The lure core of a locust migratory locust nymph attractant according to claim 3, wherein: the soaking temperature in the step (2) is 20-30 ℃.
6. The locust migratory nymph attractant and the application of the locust migratory nymph attractant in the field of locust control are characterized in that: the locust nymph attractant is used for attracting locusta migratoria nymphs to cause a tropism reaction of the locusta migratoria nymphs.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010182761.9A CN111226930A (en) | 2020-03-16 | 2020-03-16 | Locust migratory locust nymph attractant and bait and application thereof |
| CN202010671735.2A CN111587881A (en) | 2020-03-16 | 2020-07-13 | Green locust attractant for pesticide control and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010182761.9A CN111226930A (en) | 2020-03-16 | 2020-03-16 | Locust migratory locust nymph attractant and bait and application thereof |
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| CN111226930A true CN111226930A (en) | 2020-06-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010182761.9A Pending CN111226930A (en) | 2020-03-16 | 2020-03-16 | Locust migratory locust nymph attractant and bait and application thereof |
| CN202010671735.2A Withdrawn CN111587881A (en) | 2020-03-16 | 2020-07-13 | Green locust attractant for pesticide control and application thereof |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010671735.2A Withdrawn CN111587881A (en) | 2020-03-16 | 2020-07-13 | Green locust attractant for pesticide control and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111903676A (en) * | 2020-06-27 | 2020-11-10 | 内蒙古大学 | Attractant for pollinating insects, method for improving plant maturing rate and application thereof |
| CN111937873A (en) * | 2020-08-31 | 2020-11-17 | 北京嘉景生物科技有限责任公司 | Locust feeding stimulant and preparation method and application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113545359A (en) * | 2021-07-21 | 2021-10-26 | 张昕然 | Grasshopper attractant for agriculture and forestry and preparation and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102239847A (en) * | 2010-05-14 | 2011-11-16 | 中国农业大学 | Locusta migratoria manilensis locust nymph attractant and preparation method thereof |
-
2020
- 2020-03-16 CN CN202010182761.9A patent/CN111226930A/en active Pending
- 2020-07-13 CN CN202010671735.2A patent/CN111587881A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111903676A (en) * | 2020-06-27 | 2020-11-10 | 内蒙古大学 | Attractant for pollinating insects, method for improving plant maturing rate and application thereof |
| CN111937873A (en) * | 2020-08-31 | 2020-11-17 | 北京嘉景生物科技有限责任公司 | Locust feeding stimulant and preparation method and application thereof |
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| Publication number | Publication date |
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| CN111587881A (en) | 2020-08-28 |
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