CN111208127A

CN111208127A - Nitrite rapid detection test paper

Info

Publication number: CN111208127A
Application number: CN202010083667.8A
Authority: CN
Inventors: 张雅莉; 高俊海; 富玉
Original assignee: Pony Test Co
Current assignee: Pony Test Co
Priority date: 2020-01-21
Filing date: 2020-01-21
Publication date: 2020-05-29

Abstract

This patent belongs to food safety short-term test field, relates to a nitrite short-term test paper, including collapsible bottom plate, test paper A, test paper B three, test paper A and test paper B symmetrical position through special treatment are in the both sides of the well broken line of collapsible bottom plate. When the test paper is used, a drop of extraction liquid is dropped on the test paper A, after 10s, the foldable bottom plate is folded along the folding line, and the test paper A is completely contacted with the test paper B; after 10s, the bottom plate is opened, and the nitrite in the sample can be rapidly and semi-quantitatively detected by contrasting a standard colorimetric card. The method is simple and high in sensitivity, and can fully meet the requirements of field detection and laboratory detection.

Description

Nitrite rapid detection test paper

Technical Field

This patent belongs to food safety short-term test field, relates to a nitrite short-term test paper, especially relates to a nitrite short-term test paper that the principle is simple, sensitivity is high.

Background

Nitrite is often used in food processing as a color fixative or preservative. However, relevant research data indicate that nitrite has a certain carcinogenic effect, and excessive intake of nitrite may cause threat and harm to human health. Thus, national standards set strict regulations on the nitrite content of different food products. The on-site rapid detection of nitrite in food has important significance for food safety supervision.

At present, the rapid detection method of nitrite is mainly based on the' naphthalene ethylene diamine hydrochloride method (KJ201704) for rapidly detecting nitrite in food released by the State food and drug administration, and a method for rapidly detecting nitrite in food by using a liquid reagent is recorded in the method. However, liquid reagents are easy to leak, potential safety hazards exist in transportation, and meanwhile, the product composition is complex and occupies space. Meanwhile, related researches also exist for preparing the test paper by soaking the two reagents in a directly mixed solvent, but the color development effect is not obvious when the nitrite concentration is low, and the problems of concentration ratio of the two reagents and the like can be confirmed by complicated groping conditions.

Therefore, the development of the nitrite rapid detection test paper with simple principle and high sensitivity has important significance.

Disclosure of Invention

The test paper has the beneficial effects that the test paper for rapidly detecting the nitrite, which is simple in principle and high in sensitivity, can be provided.

In order to realize above-mentioned function, the utility model relates to a nitrite short-term test paper which is characterized in that, including collapsible bottom plate (1), test paper A (2), test paper B (3). The test paper A (2) and the test paper B (3) are symmetrically positioned on two sides of the middle folding line (4) of the foldable bottom plate (1); the test paper A (2) is prepared by soaking and drying 0.4-1.0% of sulfanilic acid solution, and the test paper B (3) is prepared by soaking and drying 0.2-0.5% of naphthyl ethylenediamine hydrochloride solution.

Optimally, the detection limit of the method for detecting nitrite by using the test paper is 2.0 mg/kg.

Has the advantages that:

1. the test paper is simple, safe, efficient and convenient, and the detection result has better accuracy and precision and meets the requirement of rapid detection of nitrite in food.

2. The test paper is easy to store, has long quality guarantee period, can be directly used as a commodity, and commercializes a detection technology.

3. The test paper does not need complicated operation and professional equipment, has no requirement on detection places, and can meet the requirements of on-site rapid detection or laboratory detection of departments such as individuals, enterprises, quality supervision, industrial and commercial departments and the like.

Drawings

FIG. 1 is a schematic view of the appearance

FIG. 2 is a schematic diagram of the process of use

Wherein: 1. a foldable bottom panel; 2. test paper A; 3. test paper B; 4. and a middle broken line.

FIG. 3 is a schematic diagram of a standard color chart

Detailed Description

For a detailed description of the technical content, the achieved objects and effects of the present patent, the following description is taken in conjunction with the accompanying drawings and embodiments:

example one

a. Sample pretreatment: cutting the sample, mixing, weighing 5g, adding 5mL of water, shaking, mixing, standing, and soaking for 15 min;

b. color development: dripping 1 drop of supernatant on the test paper A (2), folding the foldable bottom plate (1) along the middle folding line (4) after 10 seconds, and enabling the test paper A (2) to be in complete contact with the test paper B (3);

c. color comparison: after 10s, the foldable bottom plate (1) is opened, the color of the test paper B (3) is observed, and the content range of the nitrite in the sample is read by contrasting with a standard colorimetric card.

The above examples are provided for the purpose of illustration and are not intended to be limiting; it should be noted that various changes and modifications can be made by those skilled in the art without departing from the scope of the patent concept, and these changes and modifications are all within the scope of the patent; therefore, all equivalent changes and modifications made within the scope of the claims of the present patent are intended to be covered by the claims of the present patent.

Claims

1. A test paper for rapidly detecting nitrite is characterized by comprising a foldable bottom plate (1), a test paper A (2) and a test paper B (3); the test paper A (2) and the test paper B (3) are symmetrically positioned on two sides of the middle folding line (4) of the foldable bottom plate (1); the test paper A (2) is prepared by soaking and drying 0.4-1.0% of sulfanilic acid solution, and the test paper B (3) is prepared by soaking and drying 0.2-0.5% of naphthyl ethylenediamine hydrochloride solution.

2. The test strip for rapidly detecting nitrite according to claim 1, wherein the detection limit of the method for detecting nitrite by the test strip is 2.0 mg/kg.