CN111184789A - Chinese herbal medicine prescription for preventing and treating sheep pregnancy toxemia and application thereof - Google Patents

Chinese herbal medicine prescription for preventing and treating sheep pregnancy toxemia and application thereof Download PDF

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Publication number
CN111184789A
CN111184789A CN202010020910.1A CN202010020910A CN111184789A CN 111184789 A CN111184789 A CN 111184789A CN 202010020910 A CN202010020910 A CN 202010020910A CN 111184789 A CN111184789 A CN 111184789A
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chinese herbal
herbal medicine
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pregnancy
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买占海
罗生金
况玲
卢亚宾
李建龙
侯宇
周银萍
刘媛媛
赵璐
沙娅·奴尔兰
张毅
刘丹丹
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Xinjiang Agricultural University
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Xinjiang Agricultural University
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    • A61K36/18—Magnoliophyta (angiosperms)
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Abstract

The invention discloses a Chinese herbal medicine prescription for preparing and preventing sheep pregnancy toxemia and application thereof, wherein 4-8 parts of astragalus root, 2-6 parts of honey-fried licorice root, 5-9 parts of codonopsis pilosula, 3-7 parts of angelica, 1-5 parts of dried orange peel, 4-8 parts of radix bupleuri, 2-6 parts of bighead atractylodes rhizome, 2-6 parts of scutellaria baicalensis, 4-8 parts of fenugreek seed, 1-5 parts of semen cuscutae, 3-7 parts of white peony root, 1-5 parts of ligusticum wallichii and 4-8 parts of poria cocos are adopted to prepare the Chinese herbal medicine prescription for preventing and treating sheep pregnancy toxemia, the Chinese herbal medicine prescription can effectively prevent and treat sheep pregnancy toxemia, no sheep abortion and sheep pregnancy toxemia appear after the Chinese herbal medicine prescription is taken, the Chinese herbal medicine prescription has good effect on increasing of the birth weight of a single lamb, and the blood biochemical index detection result shows that the Chinese herbal, and the immunity of the pregnant ewe can be well improved, so that the sheep pregnancy induced toxemia prevention and treatment agent has wide practical value.

Description

Chinese herbal medicine prescription for preventing and treating sheep pregnancy toxemia and application thereof
Technical Field
The invention relates to the technical field of research and development of traditional Chinese veterinary medicines. In particular to the technical field of Chinese herbal medicine formulas for preventing and treating sheep pregnancy induced toxemia.
Background
Xinjiang is a famous animal husbandry province in China, the mutton yield of Xinjiang is only second to that of inner Mongolia, the Xinjiang lives on the 2 nd position in China, the 3 rd position in China is occupied by all people, the consumption of meat is 29kg in 2010, the number of sheep stocked is 2 nd position in China, the total output value of sheep farming accounts for more than 60% of the total output value of the Xinjiang animal husbandry, and the mutton is ranked first among various livestock and poultry meat in Xinjiang. In recent years, good varieties with high reproduction rate are vigorously introduced in Xinjiang to improve the yield of mutton, but due to the lack of corresponding feeding and management technologies, insufficient treatment experience of personnel and the like, a plurality of diseases, especially sheep toxemia of pregnancy, are generated, the damage is extremely large, and the development of the Xinjiang sheep industry is severely restricted. Therefore, the research and development of a medicine for treating and preventing sheep pregnancy induced toxemia becomes a first priority, in recent years, the problems of drug resistance, drug residues, hormone residues and the like of western medicines are more and more serious, in the livestock breeding industry, people pay more and more attention to traditional Chinese herbal medicines, and the Chinese herbal medicines have the advantages of no drug resistance, no residues, rich trace elements, rich vitamins and the like which are incomparable with the western medicines.
Disclosure of Invention
Aiming at the technical current situation that the mode for preventing and treating sheep pregnancy induced toxemia is high in cost and easy to remain in the prior art, no report on a Chinese herbal medicine prescription specially used for preparing a Chinese herbal medicine prescription for preventing and treating sheep pregnancy induced toxemia is found in the prior art. The invention aims to provide a Chinese herbal medicine prescription for preventing and treating sheep pregnancy toxemia and application thereof, which takes astragalus root, honey-fried licorice root, pilose asiabell root, Chinese angelica, dried orange peel, Chinese thorowax root, largehead atractylodes rhizome, baical skullcap root, common fenugreek seed, south dodder seed, white paeony root, Szechuan lovage rhizome and Indian buead as main components, creatively provides the Chinese herbal medicine prescription for preventing and treating the sheep pregnancy toxemia and application thereof, compared with the prior art, the Chinese herbal medicine prescription can tonify qi and blood, warm liver, tonify spleen and condition stomach channels, not only can protect fetus of animals in the treatment process, but also can protect health, the Chinese herbal medicine is prepared into powder, decocted and then mixed with the daily ration of sheep, orally administered, the ewe is administered for 5 days in each treatment period, the effects of the Chinese herbal medicine are evaluated by analyzing the morbidity of the pregnant ewe, the newborn, the method has wide practicability for preventing and treating sheep pregnancy toxemia and has great potential value for the development of sheep industry.
The invention provides a Chinese herbal medicine prescription for preventing and treating sheep pregnancy induced toxemia, which comprises astragalus, honey-fried licorice root, codonopsis pilosula, angelica, dried orange peel, radix bupleuri, bighead atractylodes rhizome, scutellaria, fenugreek seed, semen cuscutae, white paeony root, ligusticum wallichii and poria cocos.
Preferably, the Chinese herbal medicine prescription for preparing the medicine for preventing and treating sheep pregnancy induced toxemia comprises, by weight, 4-8 parts of astragalus membranaceus, 2-6 parts of honey-fried licorice root, 5-9 parts of codonopsis pilosula, 3-7 parts of angelica sinensis, 1-5 parts of pericarpium citri reticulatae, 4-8 parts of radix bupleuri, 2-6 parts of bighead atractylodes rhizome, 2-6 parts of scutellaria baicalensis, 4-8 parts of fenugreek, 1-5 parts of semen cuscutae, 3-7 parts of radix paeoniae alba, 1-5 parts of ligusticum wallichii and 4-8 parts of poria cocos.
Preferably, the Chinese herbal medicine prescription for preventing and treating sheep pregnancy induced toxemia comprises, by weight, 6 parts of astragalus membranaceus, 4 parts of honey-fried licorice roots, 7 parts of codonopsis pilosula, 5 parts of angelica sinensis, 3 parts of pericarpium citri reticulatae, 6 parts of radix bupleuri, 4 parts of bighead atractylodes rhizome, 4 parts of scutellaria baicalensis, 6 parts of semen cucurbitae, 3 parts of semen cuscutae, 5 parts of radix paeoniae alba, 3 parts of ligusticum wallichii and 6 parts of poria cocos.
Furthermore, the Chinese herbal medicine prescription provided by the invention is applied to the prevention and treatment of the sheep toxemia of pregnancy, the raw materials are weighed according to the weight ratio of the components, the Chinese herbal medicine prescription is crushed by a crusher, the traditional Chinese medicine powder is placed in a container, boiling water which is 3 times of the volume of the traditional Chinese medicine is poured into the container, the medicine is fully soaked, the medicine is stirred to be pasty and cooled for standby application, one dose is taken every day by the sheep with toxemia of pregnancy, and the course of each health care is 5 days.
The invention fully depends on that the radix codonopsitis has the effects of diuresis, strengthening heart, reducing blood pressure, resisting bacteria, expelling toxin, expelling pus, promoting granulation, strengthening capillary resistance, suppressing sweating and sex hormone-like effects of the radix astragali; the honey-fried licorice decoction has the treatment effects of tonifying qi, nourishing yin, activating yang and recovering pulse; radix Codonopsis has effects in enhancing immunity, dilating blood vessel, lowering blood pressure, improving microcirculation, and improving hemopoiesis; the angelica can tonify qi and blood, regulate menstruation and relieve pain; the dried orange peel can regulate qi, invigorate spleen, eliminate dampness and reduce phlegm; the bupleurum has the effects of harmonizing exterior and interior, soothing liver and invigorating yang; atractylodis rhizoma has effects of eliminating dampness, eliminating turbid pathogen, and relieving pain; scutellariae radix has effects of clearing heat, eliminating dampness, clearing pathogenic fire, removing toxic substances, stopping bleeding, and preventing miscarriage; the fenugreek can warm the kidney, dispel cold and relieve pain; semen Cuscutae has effects of nourishing liver and kidney, stopping nocturnal emission, reducing urination, preventing miscarriage, improving eyesight, and relieving diarrhea; radix Paeoniae alba can be used for treating limb spasm pain, headache, and giddiness; rhizoma Ligustici Chuanxiong has effects of promoting blood circulation, activating qi-flowing, dispelling pathogenic wind and relieving pain; poria is used for edema and oliguria. According to the adopted medicinal materials, the formula can be formed, the Chinese herbal medicine formula is not selected at will, particularly, the specific dosage ratio is adopted according to the experimental verification, the technical effect of remarkable preparation and prevention of the sheep pregnancy induced toxemia is ensured, and the selected medicinal materials and the selected dosage ratio are obtained through series of experiments.
The invention provides astragalus, honey-fried licorice root, codonopsis pilosula, angelica, dried orange peel, radix bupleuri, bighead atractylodes rhizome, scutellaria, fenugreek seed, semen cuscutae, radix paeoniae alba, ligusticum wallichii and poria cocos which are compatible according to a specific proportion, and the medicines are combined according to a specific dosage proportion and complement each other, so that the effects of promoting qi and blood circulation, warming meridians and dispelling cold are achieved. The Chinese herbal medicine prescription for preventing and treating sheep pregnancy toxemia has the advantages of brand new composition, quick response, good curative effect, stable efficacy and low cost, is free from toxic and side effects in use, basically free from relapse, low in cost and convenient to apply, and has a huge application prospect.
By implementing the specific invention content of the invention, the following effects can be achieved:
(1) clinical observation proves that the Chinese herbal medicine prescription for preventing and treating sheep pregnancy induced toxemia is a Chinese herbal medicine prescription which is brand new in composition, quick in curative effect, good in curative effect, stable in efficacy, low in recurrence rate and low in cost and is used for preventing and treating sheep pregnancy induced toxemia.
(2) Clinical observation shows that the birth weight of a single lamb in a test group is higher than that of a single lamb in a control group, the birth weight of the single lamb in a later stage of pregnancy is remarkably improved (P <0.05), and the birth weight of the single lamb in a whole course group is remarkably improved (P <0.01), so that the compound Chinese herbal medicine has a good effect on the increase of the birth weight of the single lamb.
(3) Clinical observation shows that the total number of white blood cells, the lymphocyte ratio, the intermediate cell ratio and the granulocyte ratio in a test group are remarkably improved (P is less than 0.05), and the improvement is in a normal range, so that the Chinese herbal medicine for supplementing qi and blood and tonifying liver and kidney can well improve the immunological competence of pregnant ewes. The results of blood biochemical index measurements show that some indexes are in a normal range although the indexes are obviously improved. The Chinese herbal medicine has no toxicity to pregnant ewes.
(4) Clinical observation shows that the secretion level of TNF- α in serum of a pregnant sheep in different gestation periods is lower than that of a control group in different gestation periods, so that the Chinese herbal medicine reduces the secretion level of TNF- α, and is beneficial to maintaining the pregnancy state of pregnant ewes.
Detailed Description
The present invention will be described below by way of examples, but the present invention is not limited to the following examples.
The equipment and materials involved in the invention: biochemical analyzer (Edes VT8008), blood conventional analyzer (Shenzhen Shangpukang electronic Limited), high-speed centrifuge (TG16W type, Shanghai), pipette (Eppendorf), stopwatch, blood taking needle, EDTA blood collection tube, plateau common blood collection tube, H/P/cosmos portable blood lactic acid analyzer, Hitachi Z180 full-automatic biochemical analyzer
All the raw materials and auxiliary materials selected in the invention are well known in the art and are not limited to the implementation of the test.
The first embodiment is as follows: chinese herbal medicine prescription for preventing and treating sheep pregnancy toxemia
The invention provides a Chinese herbal medicine prescription for preventing and treating sheep pregnancy induced toxemia, which comprises astragalus, honey-fried licorice root, codonopsis pilosula, angelica, dried orange peel, radix bupleuri, bighead atractylodes rhizome, scutellaria, fenugreek seed, semen cuscutae, white paeony root, ligusticum wallichii and poria cocos.
The invention specifically provides a Chinese herbal medicine prescription for preparing a medicine for preventing and treating sheep pregnancy induced toxemia, which comprises, by weight, 4-8 parts of astragalus membranaceus, 2-6 parts of honey-fried licorice root, 5-9 parts of codonopsis pilosula, 3-7 parts of angelica sinensis, 1-5 parts of dried orange peel, 4-8 parts of radix bupleuri, 2-6 parts of bighead atractylodes rhizome, 2-6 parts of scutellaria baicalensis, 4-8 parts of fenugreek, 1-5 parts of semen cuscutae, 3-7 parts of radix paeoniae alba, 1-5 parts of ligusticum wallichii and 4-8 parts of poria cocos.
The Chinese herbal medicine prescription is applied to preparing the medicine for preventing and treating the pregnancy induced hypertension, the raw materials are weighed according to the weight ratio of the components, the Chinese herbal medicine prescription is crushed by a crusher, the powdered traditional Chinese medicine is placed in a container, boiling water which is 3 times of the volume of the traditional Chinese medicine is poured into the container, the medicine is fully soaked, the medicine is stirred to be pasty and cooled for standby application, one dose is taken every day by the pregnancy induced hypertension sheep, and the health care course is 5 days.
Example two:
the Chinese herbal medicine prescription is prepared from 4g of astragalus root, 2g of honey-fried licorice root, 5g of codonopsis pilosula, 3g of angelica, 1g of dried orange peel, 4g of radix bupleuri, 2g of bighead atractylodes rhizome, 2g of scutellaria baicalensis, 4g of fenugreek, 1g of semen cuscutae, 3g of white paeony root, 1g of ligusticum wallichii and 4g of poria cocos, and is used for preventing and treating sheep pregnancy toxemia.
Example three:
the Chinese herbal medicine prescription is prepared from 5g of astragalus root, 3g of honey-fried licorice root, 6g of codonopsis pilosula, 4g of angelica, 2g of dried orange peel, 5g of radix bupleuri, 3g of bighead atractylodes rhizome, 3g of scutellaria baicalensis, 5g of fenugreek, 2g of semen cuscutae, 4g of white paeony root, 2g of ligusticum wallichii and 5g of poria cocos.
Example four:
the Chinese herbal medicine prescription is prepared from 6g of astragalus root, 4g of honey-fried licorice root, 7g of codonopsis pilosula, 5g of angelica, 3g of dried orange peel, 6g of radix bupleuri, 4g of bighead atractylodes rhizome, 4g of scutellaria baicalensis, 6g of fenugreek, 3g of semen cuscutae, 5g of white paeony root, 3g of ligusticum wallichii and 6g of poria cocos, and is used for preventing and treating sheep pregnancy toxemia.
Example five:
the Chinese herbal medicine prescription is prepared from 7g of astragalus, 5g of honey-fried licorice root, 8g of codonopsis pilosula, 6g of angelica, 4g of dried orange peel, 5g of radix bupleuri, 5g of bighead atractylodes rhizome, 5g of scutellaria baicalensis, 7g of fenugreek, 4g of semen cuscutae, 6g of radix paeoniae alba, 4g of ligusticum wallichii and 7g of poria cocos, and is used for preventing and treating sheep pregnancy toxemia.
Example six:
the Chinese herbal medicine prescription is prepared from 8g of astragalus root, 6g of honey-fried licorice root, 9g of codonopsis pilosula, 7g of angelica, 5g of dried orange peel, 8g of radix bupleuri, 6g of bighead atractylodes rhizome, 6g of scutellaria baicalensis, 8g of fenugreek, 5g of semen cuscutae, 7g of white paeony root, 5g of ligusticum wallichii and 8g of poria cocos, and is used for preventing and treating sheep pregnancy toxemia.
Example seven: application of Chinese herbal medicine prescription in preventing and treating sheep pregnancy toxemia
1. Grouping animals
Randomly selecting 25 ewes in the early stage of pregnancy as an early stage of pregnancy group, and selecting the rest ewes in the early stage of pregnancy as an early stage of pregnancy control group; randomly selecting 20 ewes in the middle of gestation as a middle group of gestation, and taking the remaining ewes in the middle of gestation as a middle control group of gestation; randomly selecting 20 ewes at the later stage of gestation as a group at the later stage of gestation, and using the remaining ewes at the later stage of gestation as a control group at the later stage of gestation; the whole-course pregnant ewes are randomly selected from 25 whole-course groups (whole-course early group, whole-course middle group and whole-course later group), and the rest whole-course pregnant ewes are whole-course control groups (whole-course early control group, whole-course middle control group and whole-course later control group).
2. Experimental methods
The method comprises the steps of carrying out a health care treatment course on a ewe group in the early stage of pregnancy, a ewe group in the middle stage of pregnancy and a ewe group in the later stage of pregnancy, wherein the whole course of the three health care treatment courses comprise a whole course early stage group, a whole course middle stage group and a whole course later stage group, each health care treatment course is 5 days, uniformly shaking blood collected before and after administration of test groups at each time point, sucking a certain amount of blood, placing the blood into a full-automatic blood cell analyzer to measure related indexes, collecting the blood without the addition of an anticoagulant at 4 ℃ overnight, centrifuging at 3000rpm for 10min, sucking serum to carry out biochemical examination on liver function and kidney function indexes, carrying out TNF- α and IGF-I immune detection, completing TNF- α and IGF-I immune detection with assistance of Nanjing ao biological Limited company, and carrying out single-factor significance analysis on detection results by using SPSS.
Indexes to be measured: the disease condition of ewes (ewe abortion rate, ewe pregnancy toxemia disease number of ewes) and the birth weight of lambs (within 12 hours).
Ewe miscarriage yield (%). abortus ewe count/gestation ewe count 100%
3. Results and analysis
3.1 Effect of Compound Chinese herbal medicine on the onset of pregnancy in ewes
As shown in the table 1, the compound Chinese herbal medicine is used for the disease condition table of ewes in the early stage of pregnancy, the number of actual born ewes in the period is totally 100, the number of born ewes in a control group is 84, the number of aborted ewes is 7, and the number of ewe with toxemia of pregnancy is 8; the number of the born sheep in the early pregnancy group is 16, and no abortion and pregnancy toxemia appear in the sheep.
Table 1: compound Chinese herbal medicine for onset of ewe in early gestation
Figure BDA0002360712620000081
As can be seen from the table 1-2, the number of actually born sheep in the period is 51 in total, the number of born sheep in the control group is 39, the number of aborted sheep is 7, and the number of the ewe with the onset of gestational toxemia is 2; the number of the born sheep in the early pregnancy group is 12, and no abortion and pregnancy toxemia appear in the sheep.
Table 2: compound Chinese herbal medicine for disease condition of ewe in middle of gestation
Figure BDA0002360712620000082
Figure BDA0002360712620000091
As can be seen from Table 3, in the late stage of pregnancy, the number of actually born sheep is 44, the number of born sheep in the control group is 34, the number of born sheep is 7, and the number of the sheep with pregnancy toxemia disease in the ewe is 6; the number of the born sheep in the early pregnancy group is 10, and no abortion and pregnancy toxemia appear in the sheep.
Table 3: compound Chinese herbal medicine for disease condition of ewe in later stage of pregnancy
Figure BDA0002360712620000092
As can be seen from tables 1-4, the total number of actual born sheep in this stage is 91, the number of born sheep in the control group is 75, the number of aborted sheep is 8, and the number of the ewe with pregnancy toxemia disease is 4; the number of the born sheep in the early pregnancy group is 16, and no abortion and pregnancy toxemia appear in the sheep.
Table 4: the compound Chinese herbal medicine is used for the disease condition of the whole group of ewes
Figure BDA0002360712620000093
3.2 Effect of Compound Chinese herbal medicine on the birth weight of lamb
The influence of the compound Chinese herbal medicine provided by the invention on the birth weight of single lambs in the early gestation group is shown in Table 5, and the early gestation group and the control group are not obvious (P is more than 0.05).
Table 5: influence of compound Chinese herbal medicine on birth weight of single lamb in early pregnancy group
Group of Sample size/n Birth weight of lamb
Control group for early pregnancy 5 2.840±0.8849Aa
Group of early pregnancy 7 3.457±1.018Aa
Note: in the table, different capital letters indicate significant differences (P <0.05) and different lowercase letters indicate significant differences (P < 0.01). The same applies below.
The influence of the compound Chinese herbal medicine provided by the invention on the birth weight of single lambs in the middle gestation group is shown in Table 6, and the middle gestation group and the control group are not obvious (P is more than 0.05).
Table 6: influence of compound Chinese herbal medicine on birth weight of single lamb in middle-term gestation group
Group of Sample size/n Birth weight of lamb
Control group in middle of gestation 3 3.383±0.881Aa
Middle group of pregnancies 6 3.492±0.317Aa
The influence of the compound Chinese herbal medicine on the birth weight of a single lamb in the later group of pregnancy is shown in Table 7, and after the compound Chinese herbal medicine is taken, the birth weight of the lamb is obviously increased, and the difference between the later group of pregnancy and the control group is obvious (P is less than 0.05).
Table 7: influence of compound Chinese herbal medicine on birth weight of single lamb in later stage of pregnancy
Group of Sample size/n Birth weight of lamb
Control group in later stage of pregnancy 3 3.950±1.476Ba
Group of late gestation 3 4.697±1.113Aa
The influence of the compound Chinese herbal medicine provided by the invention on the birth weight of a single lamb in the whole course group is shown in Table 8, the birth weight of the lamb is obviously increased after the medicine is taken, and the difference between the whole course group and a control group is obvious (P is less than 0.05).
Table 8: influence of compound Chinese herbal medicine on birth weight of single lamb in whole course group
Figure BDA0002360712620000101
Figure BDA0002360712620000111
The comparison of the birth weight of single lamb among the test groups is shown in table 9, after the application of the drug, the birth weight of the lamb is obviously increased, and the difference between the later group and the early group is obvious (P is less than 0.05); the whole course group and the early pregnancy group have very obvious difference (P < 0.01).
Table 9: comparison of Single lamb birth weight between test groups
Group of Sample size/n Birth weight of lamb
Group of early pregnancy 5 2.840±0.885Bb
Middle group of pregnancies 6 3.492±0.317ABab
Group of late gestation 3 3.700±0.265Aab
Whole course group 9 3.933±0.466Aa
The influence of the compound Chinese herbal medicine on the birth weight of the lambs in the early gestation group is shown in the table 10, and after the compound Chinese herbal medicine is taken, the birth weight of the lambs is not obviously changed, and the birth weight of the lambs in the early gestation group and the birth weight of the lambs in the control group are not obvious (P is more than 0.05).
Table 10: influence of compound Chinese herbal medicine on newborn weight of group lambs in early gestation period
Group of Sample size/n Birth weight of lamb
Control group for early pregnancy 7 3.143±0.479Aa
Group of early pregnancy 11 2.909±0.532Aa
The influence of the Chinese herbal medicines for tonifying qi and blood and tonifying liver and kidney on the birth weight of the lambs in the middle gestation group is shown in table 11, after the medicine is taken, the birth weight of the lambs does not change obviously, and the birth weight of the lambs in the middle gestation group and the birth weight of the lambs in the control group are not obvious (P is more than 0.05).
Table 11: influence of compound Chinese herbal medicine on birth weight of lambs in middle-term gestation group
Group of Sample size/n Birth weight of lamb
Control group in middle of gestation 5 2.900±0.854Aa
Middle group of pregnancies 3 2.783±0.751Aa
The influence of the compound Chinese herbal medicine on the birth weight of the lambs in the later group of pregnancy is shown in Table 12, after the compound Chinese herbal medicine is taken, the birth weight of the lambs is not obviously changed, and the birth weight of the lambs in the later group of pregnancy and the birth weight of the lambs in the control group are not obvious (P is more than 0.05).
Table 12: influence of compound Chinese herbal medicine on newborn weight of group lambs in later stage of pregnancy
Group of Sample size/n Birth weight of lamb
Control group in later stage of pregnancy 3 2.533±0.382Aa
Group of late gestation 8 2.981±0.392Aa
The influence of the compound Chinese herbal medicine provided by the invention on the birth weight of the lambs in the whole course group is shown in Table 13, after the compound Chinese herbal medicine is taken, the birth weight of the lambs is not obviously changed, and the whole course group and the control group are not obvious (P is more than 0.05).
Table 13: influence of compound Chinese herbal medicine on birth weight of whole-course group lambs
Group of Sample size/n Birth weight of lamb
Whole course control group 6 3.175±0.477Aa
Whole course group 5 2.800±0.292Aa
Comparison of the initial weight of the lambs among the test groups revealed that none of the test groups were significant (P >0.05) compared to the control group, as shown in Table 14.
Table 14: comparison of lamb birth weight between test groups
Group of Sample size/n Birth weight of lamb
Group of early pregnancy 11 2.909±0.532Aa
Middle group of pregnancies 3 2.783±0.751Aa
Group of late gestation 8 2.981±0.392Aa
Whole course group 5 2.800±0.292Aa
3.3 Effect of Compound Chinese herbal medicine on physiological indexes of pregnant ewe blood
The influence of the compound Chinese herbal medicine provided by the invention on early pregnancy physiological indexes of pregnant ewes is shown in Table 15, and the difference between an early pregnancy group and a control group is very obvious (P is less than 0.01) in the aspect of lymphocyte ratio; the granulocyte ratio is remarkably different from the control group in the early gestation group (P < 0.05); on other indexes, the difference of each group is not significant (P > 0.05).
Table 15: influence of compound Chinese herbal medicine on physiological index of early pregnancy
Group of Control group for early pregnancy Group of early pregnancy
Sample size/n 12 14
Total white blood cell count (109/L) 14.525±3.824Aa 13.079±3.134Aa
Lymphocyte ratio (%) 81.517±3.227Aa 74.871±3.748Bb
Ratio of intermediate cells (%) 7.642±1.676Aa 8.100±1.722Aa
Granulocyte ratio (%) 10.842±2.087Ba 17.029±3.111Aa
Total number of erythrocytes (1012/L) 6.715±0.536Aa 6.289±0.888Aa
Hemoglobin (g/L) 100.62±32.879Aa 102.57±16.468Aa
Packed cell volume (%) 28.458±2.045Aa 26.364±3.996Aa
Total number of platelets (109/L) 199.58±49.309Aa 198.29±50.130Aa
The influence of the compound Chinese herbal medicine provided by the invention on the early physiological indexes of the pregnant ewes is shown in Table 16, and the difference between the test group and the control group is not significant (P is more than 0.05) in all the physiological indexes.
Table 16: influence of compound Chinese herbal medicine on physiological index in middle of gestation
Figure BDA0002360712620000131
Figure BDA0002360712620000141
The influence of the compound Chinese herbal medicine on physiological indexes in the later stage of pregnancy is shown in Table 17, after the medicine is taken, the total number of red blood cells, hemoglobin, the total number of red blood cells and the total number of blood platelets are obviously reduced, and the difference between a control group and a group in the later stage of pregnancy is extremely obvious (P is less than 0.01); the total number of white blood cells and the lymphocyte ratio are reduced, and the difference between a control group and a later pregnancy group is obvious (P < 0.05); in other physiological indexes, the difference between the control group and the later-stage gestation group is not significant (P > 0.05).
Table 17: influence of compound Chinese herbal medicine on physiological index of later stage of pregnancy
Group of Control group in later stage of pregnancy Group of late gestation
Sample size/n 6 7
Total white blood cell count (109/L) 11.767±2.693Aa 5.700±3.778Ba
Lymphocyte ratio (%) 69.883±4.789Aa 55.429±12.365Ba
Ratio of intermediate cells (%) 12.183±3.888Aa 17.729±7.237Aa
Granulocyte ratio (%) 17.93±3.085Aa 26.84±12.665Aa
Total number of erythrocytes (1012/L) 6.483±0.719Aa 4.063±1.783Bb
Hemoglobin (g/L) 107.17±10.759Aa 70.71±32.325Bb
Packed cell volume (%) 28.867±3.797Aa 17.986±7.606Bb
Total number of platelets (109/L) 166.83±28.131Aa 104.14±59.185Bb
The influence of the compound Chinese herbal medicine provided by the invention on the whole-course early physiological indexes is shown in Table 18, the total number of leucocytes and the ratio of granulocytes are obviously increased, and the difference between the whole-course early group and a control group is obvious (P is less than 0.05); in other physiological indexes, the difference between the whole course early group and the control group is not significant (P is more than 0.05).
Table 18: influence of compound Chinese herbal medicine on whole course early physiological index
Group of Whole course early control group Whole course early group
Sample size/n 12 14
Total white blood cell count (109/L) 9.708±4.652Ba 13.671±3.157Aa
Lymphocyte ratio (%) 75.233±5.756Aa 72.607±4.542Aa
Ratio of intermediate cells (%) 11.325±2.930Aa 9.943±1.954Aa
Granulocyte ratio (%) 13.433±3.381Ba 17.450±3.297Aa
Total number of erythrocytes (1012/L) 5.288±1.844Aa 5.858±0.567Aa
Hemoglobin (g/L) 86.50±31.518Aa 94.50±9.796Aa
Packed cell volume (%) 22.508±7.875Aa 24.764±2.649Aa
Total number of platelets (109/L) 145.00±64.58Aa 176.79±47.721Aa
The influence of the compound Chinese herbal medicine on the whole-course middle-stage physiological indexes is shown in Table 19, after the medicine is taken, the total number of white blood cells and the total number of platelets are obviously increased, and the difference between the whole-course middle-stage group and a control group is very obvious (P is less than 0.01); the granulocyte ratio is obviously increased, and the difference between the whole-course metaphase group and the control group is extremely obvious (P < 0.05); the lymphocyte ratio is obviously reduced, and the difference between the control group and the whole course middle period group is extremely obvious (P < 0.01); in other physiological indexes, the difference between the whole course middle period group and the control group is not significant (P is more than 0.05).
Table 19: influence of compound Chinese herbal medicine on whole course middle period physiological index
Figure BDA0002360712620000151
Figure BDA0002360712620000161
The influence of the compound Chinese herbal medicine on the physiological indexes in the later period of the whole course is shown in the table 1-20, after the medicine is taken, the lymphocyte ratio is obviously increased, and the difference between the group in the later period of the whole course and the control group is very obvious (P is less than 0.01); the granulocyte ratio is remarkably reduced, and the difference between the whole course later period group and the control group is very remarkable (P < 0.01); the ratio of intermediate cells is obviously reduced, and the difference between the whole course later period group and the control group is obvious (P < 0.05); and in other physiological indexes, the difference between the whole course later group and the control group is not significant (P is more than 0.05).
Table 20: influence of compound Chinese herbal medicine on physiological index in later period of whole course
Group of Whole-course late control group Last group of the whole course
Sample size/n 12 14
Total white blood cell count (109/L) 8.29±2.515Aa 20.66±30.067Aa
Lymphocyte ratio (%) 58.967±2.451Bb 60.814±2.625Aa
Ratio of intermediate cells (%) 4.68±0.826Aa 3.69±0.780Ba
Granulocyte ratio (%) 36.375±1.834Aa 35.486±2.164Bb
Total number of erythrocytes (1012/L) 10.343±0.639Aa 10.264±0.854Aa
Hemoglobin (g/L) 111.08±9.728Aa 115.00±10.258Aa
Packed cell volume (%) 26.83±18.01Aa 26.79±26.07Aa
Total number of platelets (109/L) 297.92±66.527Aa 276.57±78.698Aa
3.4 Effect of Compound Chinese herbal medicine on biochemical indicators of pregnant ewe blood
The influence of the compound Chinese herbal medicine provided by the invention on the biochemical indexes of the blood of the ewe in the early stage of pregnancy can be known from table 21, and the difference between a test group and a control group is not significant (P is more than 0.05) in the aspects of various biochemical indexes.
Table 21: influence of compound Chinese herbal medicine on biochemical indexes in early pregnancy
Figure BDA0002360712620000162
Figure BDA0002360712620000171
The influence of the compound Chinese herbal medicine on the biochemical indexes of the blood of the ewe in the middle of gestation is shown in Table 22, and after the compound Chinese herbal medicine is used, the calcium ions, creatinine, phosphorus and total protein of a test group are obviously increased, and the difference between the test group and a control group is obvious (P is less than 0.05); in other biochemical indexes, the difference between the test group and the control group is not significant (P > 0.05).
Table 22: influence of compound Chinese herbal medicine on biochemical indexes in middle of gestation
Figure BDA0002360712620000172
Figure BDA0002360712620000181
The influence of the compound Chinese herbal medicine provided by the invention on the biochemical indexes of the blood of the ewe in the later stage of pregnancy can be known from the table 23, and the difference between a control group and a test group is not significant (P is more than 0.05) in the aspects of various biochemical indexes.
Table 23: influence of compound Chinese herbal medicine on biochemical indexes in later stage of pregnancy
Group of Control group in later stage of pregnancy Group of late gestation
Sample size/n 5 6
Albumin 24.000±3.317Aa 23.330±3.266Aa
Alkaline phosphatase 104.600±36.821Aa 141.830±94.724Aa
Alanine transferases 34.400±23.416Aa 34.500±17.627Aa
Amylopsin 47.200±16.162Aa 81.830±42.771Aa
Urea nitrogen in blood 2.940±1.664Aa 4.250±3.530Aa
Calcium ion 2.482±0.189Aa 2.440±0.216Aa
Creatinine 70.000±11.000Aa 49.830±25.007Aa
Blood sugar 0.532±0.409Aa 2.665±3.425Aa
Phosphorus (P) 1.842±0.300Aa 2.298±0.947Aa
Total bilirubin 4.200±4.919Aa 3.670±1.862Aa
Total protein 63.400±7.570Aa 59.000±7.950Aa
Cholesterol 0.880±0.277Aa 1.057±0.426Aa
The influence of the compound Chinese herbal medicine on the biochemical indexes of the blood of the ewes in the early whole course is shown in the table 24, and the pancreatic amylase of the test group is obviously reduced, and the difference between the control group and the test group is extremely obvious (P is less than 0.01) in the aspect of the pancreatic amylase; in other biochemical indexes, the difference between the control group and the test group is not significant (P > 0.05).
Table 24: influence of compound Chinese herbal medicine on whole course early biochemical index
Group of Whole course early control group Whole course early group
Sample size/n 5 5
Albumin 23.800±4.087Aa 23.600±3.782Aa
Alkaline phosphatase 130.600±70.188Aa 179.200±76.480Aa
Alanine transferases 32.600±20.995Aa 21.400±12.198Aa
Amylopsin 86.000±42.778Aa 20.200±13.387Bb
Urea nitrogen in blood 2.260±1.869Aa 5.560±3.097Aa
Calcium ion 2.256±0.539Aa 2.374±0.272Aa
Creatinine 44.000±22.170Aa 40.600±12.542Aa
Blood sugar 1.550±1.186Aa 3.320±1.921Aa
Phosphorus (P) 1.402±0.429Aa 2.116±0.661Aa
Total bilirubin 2.000±0.000Aa 2.000±0.000Aa
Total protein 52.200±19.292Aa 59.800±18.047Aa
Cholesterol 0.996±0.647Aa 1.184±0.624Aa
The influence of the compound Chinese herbal medicine provided by the invention on the whole-course middle-term ewe blood biochemical indexes can be known from the table 25, and the difference between a control group and a test group is not significant (P is more than 0.05) in all biochemical indexes.
Table 25: influence of compound Chinese herbal medicine on biochemical indexes in middle course
Figure BDA0002360712620000191
Figure BDA0002360712620000201
The influence of the compound Chinese herbal medicine provided by the invention on the biochemical indexes of the blood of the ewes in the later period of the whole process can be known from the table 26, and the difference between the test group and the control group is not significant (P is more than 0.05) in the aspects of various biochemical indexes.
Table 26: influence of compound Chinese herbal medicine on biochemical indexes in later period of whole course
Group of Whole-course late control group Last group of the whole course
Sample size/n 5 5
Albumin 24.400±5.128Aa 25.800±3.633Aa
Alkaline phosphatase 107.800±25.985Aa 138.000±46.771Aa
Alanine transferases 27.000±10.512Aa 28.000±14.353Aa
Amylopsin 83.600±32.347Aa 78.80±41.324Aa
Urea nitrogen in blood 2.980±0.766Aa 2.180±1.188Aa
Calcium ion 2.370±0.481Aa 2.578±0.356Aa
Creatinine 54.200±17.655Aa 61.200±9.149Aa
Blood sugar 1.466±0.783Aa 2.262±0.789Aa
Phosphorus (P) 1.532±0.264Aa 1.762±0.416Aa
Total bilirubin 2.000±0.000Aa 5.000±6.708Aa
Total protein 56.000±13.058Aa 63.800±11.009Aa
Cholesterol 1.154±0.533Aa 1.068±0.355Aa
3.5 Effect of Compound Chinese herbal medicine on cytokine of gestational ewe
The influence of the compound Chinese herbal medicine provided by the invention on the cytokine of the ewe in the early gestation period is shown in Table 27, and the difference between the group in the early gestation period and the control group is not significant (P is more than 0.05).
Table 27: influence of compound Chinese herbal medicine on cell factors of ewes in early gestation
Group of Control group for early pregnancy Group of early pregnancy
Sample size/n 5 5
IGF-I concentration (μ g/L) 8.445±0.615Aa 8.107±1.727Aa
TNF- α concentration (ng/mL) 162.111±16.625Aa 154.712±15.865Aa
The influence of the compound Chinese herbal medicine provided by the invention on the cytokine of the ewe in the middle of gestation is shown in Table 28, and the difference between the group in the middle of gestation and the control group is not significant (P is more than 0.05).
Table 28: influence of compound Chinese herbal medicine on cell factors of ewes in middle of gestation
Group of Control group in middle of gestation Middle group of pregnancies
Sample size/n 5 5
IGF-I concentration (μ g/L) 18.388±3.670Aa 16.510±1.335Aa
TNF- α concentration (ng/mL) 181.125±9.653Aa 172.158±41.894Aa
The influence of the compound Chinese herbal medicine provided by the invention on the cytokine of the ewe in the later stage of pregnancy is shown in Table 29, and the difference between the group in the later stage of pregnancy and the control group is not significant (P is more than 0.05).
Table 29: influence of compound Chinese herbal medicine on cell factors of ewes in later stage of pregnancy
Group of Control group in later stage of pregnancy Group of late gestation
Sample size/n 9 7
IGF-I concentration (μ g/L) 14.085±1.416Aa 15.367±1.205Aa
TNF- α concentration (ng/mL) 122.45±23.617Aa 130.315±16.734Aa
The influence of the compound Chinese herbal medicine provided by the embodiment on the whole course early ewe cytokine is shown in table 30, and the difference between the whole course early ewe group and the control group is not significant (P is more than 0.05).
Table 30: influence of compound Chinese herbal medicine on whole-course early ewe cell factor
Group of Whole course early control group Whole course early group
Sample size/n 8 6
IGF-I concentration (μ g/L) 7.830±1.167Aa 8.598±1.527Aa
TNF- α concentration (ng/mL) 140.860±8.440Aa 143.334±9.447Aa
The influence of the compound Chinese herbal medicine provided by the invention on the whole course middle ewe cytokine is shown in table 31, and the difference between the whole course middle-term group and the control group is not significant (P is more than 0.05).
Table 31: influence of compound Chinese herbal medicine on whole-course middle-term ewe cytokine
Group of Whole course middle period control group Middle-term group of whole course
Sample size/n 4 5
IGF-I concentration (μ g/L) 10.713±0.717Aa 9.755±0.622Aa
TNF- α concentration (ng/mL) 164.580±13.158Aa 175.261±9.889Aa
The influence of the compound Chinese herbal medicine provided by the invention on the cytokine of the ewe in the later period of the whole process is shown in Table 32, and the difference between the group in the later period of the whole process and the control group is not significant (P is more than 0.05).
Table 32: influence of compound Chinese herbal medicine on whole-course later-period ewe cell factor
Group of Whole-course late control group Last group of the whole course
Sample size/n 7 7
IGF-I concentration (μ g/L) 8.384±0.332Aa 8.145±0.662Aa
TNF- α concentration (ng/mL) 158.712±7.290Aa 153.081±17.904Aa
Example eight: application of Chinese herbal medicine prescription in preventing and treating sheep pregnancy toxemia
Based on the seventh embodiment, the Chinese herbal medicine formulas for preventing and treating the pregnancy induced toxemia in the sheep are selected from the second embodiment, the third embodiment, the fifth embodiment and the sixth embodiment, eight sheep with basically similar conditions under the same feeding conditions are selected, the sheep are taken before and after exercise, and the effect after taking the medicines is detected. The results show that: after the Chinese herbal medicine formulas for preventing and treating sheep toxemia in pregnancy prepared by the schemes provided in the second, third, fifth and sixth examples are taken by sheep, abortion of sheep and onset symptoms of sheep toxemia in pregnancy still occur.
According to the comparison of the treatment results, the Chinese herbal medicine prescription for preventing and treating sheep toxemia of pregnancy provided by the test is effective for treating and relieving sheep toxemia of pregnancy, but has the optimal effect compared with the prescription provided by the fourth embodiment.
As mentioned above, the above examples are only for describing the preferred embodiments of the test, and are not intended to limit the scope of the test, and various modifications and improvements made to the technical solutions of the test by those skilled in the art without departing from the spirit of the design of the test should fall within the protection scope determined by the test.

Claims (4)

1. A Chinese herbal medicine prescription for preparing a medicine for preventing and treating sheep pregnancy induced toxemia is characterized by comprising astragalus, honey-fried licorice root, codonopsis pilosula, angelica, dried orange peel, radix bupleuri, bighead atractylodes rhizome, scutellaria baicalensis, common fenugreek seed, semen cuscutae, white paeony root, ligusticum wallichii and poria cocos.
2. The Chinese herbal medicine prescription for preparing the medicine for preventing and treating sheep pregnancy induced toxemia according to claim 1, characterized by comprising 4-8 parts of astragalus membranaceus, 2-6 parts of honey-fried licorice root, 5-9 parts of codonopsis pilosula, 3-7 parts of angelica sinensis, 1-5 parts of pericarpium citri reticulatae, 4-8 parts of radix bupleuri, 2-6 parts of bighead atractylodes rhizome, 2-6 parts of scutellaria baicalensis, 4-8 parts of fenugreek, 1-5 parts of semen cuscutae, 3-7 parts of radix paeoniae alba, 1-5 parts of ligusticum wallichii and 4-8 parts of poria cocos in parts by weight.
3. The Chinese herbal medicine prescription for preparing the medicine for preventing and treating sheep pregnancy induced toxemia according to claim 1, characterized by comprising 6 parts by weight of astragalus, 4 parts by weight of honey-fried licorice root, 7 parts by weight of codonopsis pilosula, 5 parts by weight of angelica sinensis, 3 parts by weight of dried orange peel, 6 parts by weight of radix bupleuri, 4 parts by weight of bighead atractylodes rhizome, 4 parts by weight of scutellaria baicalensis, 6 parts by weight of fenugreek, 3 parts by weight of semen cuscutae, 5 parts by weight of white peony root, 3 parts by weight of ligusticum wallichii.
4. The use of a Chinese herbal medicine formulation as claimed in claim 1 for the manufacture of a medicament for the treatment of toxemia of pregnancy in sheep, wherein the Chinese herbal medicine formulation is crushed by a crusher, the powdered Chinese herbal medicine is placed in a container, boiling water equivalent to 3 times the volume of the Chinese herbal medicine is poured in, the medicine is thoroughly soaked, stirred to be pasty, and cooled for use, and the toxemia of pregnancy sheep is taken one dose per day with 5 days as a course of health care.
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* Cited by examiner, † Cited by third party
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王壮成等: "中西医结合辨证治疗驴妊娠毒血症", 《现代农业科技》 *

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Application publication date: 20200522