CN111172212A - Fermentation method of high-content polyglutamic acid - Google Patents
Fermentation method of high-content polyglutamic acid Download PDFInfo
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Abstract
The invention discloses a fermentation method of high-content polyglutamic acid. The fermentation method comprises the following steps: culturing the bacillus subtilis producing polyglutamic acid in a primary seed culture medium to OD600Value 10-15, transferring to secondary seed culture to OD600And (3) the pH value is 28-36, inoculating the mixture into a fermentation medium, dropwise adding sulfuric acid to control the pH value of the fermentation liquor to be kept at the point until the fermentation is finished when the pH value of the fermentation liquor is lowered to the lowest point and starts to rise, and simultaneously starting to perform intermittent feeding until the fermentation is finished, wherein the fermentation period is generally 45-60 hours. The fermentation method firstly ensures the activity of the seeds, can grow quickly after being inoculated into a fermentation culture medium, shortens the fermentation period and reduces the production cost; under the relatively low pH environment, the viscosity of the polyglutamic acid fermentation liquor can be reduced, and the dissolved oxygen value of the fermentation liquor is improved, so that the production of high-content polyglutamic acid is realized. Hair brushThe content of the obtained polyglutamic acid fermentation liquor can reach 50-65 g/L.
Description
Technical Field
The invention relates to the technical field of microbial fermentation, in particular to a fermentation production method of high-content polyglutamic acid.
Background
Polyglutamic acid is a kind of biostimulant, is a water-soluble, degradable high molecular and anionic polypeptide polymer formed by synthesizing gamma-amido bond by D-or L-glutamic acid through microorganisms, and the molecular weight is from 50KDa to 20000 KDa. A large number of free carboxyl groups on the main chain of the polyglutamic acid molecule can be subjected to reactions such as crosslinking, chelation, derivatization and the like. According to research reports, the polyglutamic acid can be used as a fertilizer, a pesticide synergist, a water-retaining agent, a heavy metal ion adsorbent, a flocculating agent, a slow release agent, a drug carrier and the like. In recent years, polyglutamic acid has been widely used due to its specific role in agricultural fertilizers. The fertilizer is mainly expressed in the aspects of retaining water and fertilizer, improving the utilization rate of the fertilizer, promoting the growth of crops, resisting stress, increasing yield, improving the quality of fruits, adjusting the pH value of soil and the like.
However, in terms of the level of polyglutamic acid fermentation in the industry at present, the content of products obtained by fermentation is relatively low, the fermentation period is long, the obtained high-content products are generally limited to laboratory bench tests, and the content cannot meet the requirements in actual large-scale production. Rojun et al, adding n-dodecane and hydrogen peroxide into the culture medium to increase the oxygen content in the fermentation broth, thereby realizing the high yield of polyglutamic acid, wherein the maximum fermentation volume is 100L fermentation tank, the yield of the polyglutamic acid can reach 57.3g/L, the fermentation period is 74h, and the patent publication number is CN 1106636240; gekko Swinhonis et al have been disclosed in patent publication No. CN103695485, in which the fermentation period is 72 hours, by adding sodium nitrate to the medium to adjust the activity of glutamate synthetase, thereby achieving a polyglutamic acid yield of 45-55 g/L.
Factors influencing the low yield of the bacterial fermentation polyglutamic acid are mainly that the fermentation liquor becomes viscous in the middle and later stages of fermentation, the growth of the bacterial is too slow due to low dissolved oxygen, and the synthesis rate of the polyglutamic acid is reduced. How to solve the problem is the key to improve the fermentation yield of the polyglutamic acid.
Disclosure of Invention
Aiming at the problems, the invention provides a fermentation production method of high-content polyglutamic acid, the content of the polyglutamic acid in fermentation liquor can reach 50-65g/L, and large-scale production can be realized.
The invention relates to a fermentation method of high-content polyglutamic acid. The fermentation process comprises the following steps: culturing the bacillus subtilis producing polyglutamic acid in a primary seed culture medium to OD600Value 10-15, transferring to secondary seed culture to OD600The value is 28-36, the fermentation medium is inoculated, the culture temperature is 39 ℃, when the pH value of the fermentation liquid is lowered to the lowest point and starts to rise, sulfuric acid is dripped to control the pH value of the fermentation liquid to be kept at the point until the fermentation is finished, meanwhile, intermittent feeding is started until the fermentation is finished, the fermentation period is generally 45-60 hours, and the content of the polyglutamic acid can reach 50-65 g/L.
The strain used in the invention is bacillus subtilis, and is Shandong peptide and a strain preserved by biotechnology limited company. The first-stage seed culture medium and the second-stage seed culture medium have the same components, and specifically comprise the following components: 4-12g/L of yeast extract, 30-40g/L of glucose, 24-33g/L of sodium glutamate, 3-6g/L of monopotassium phosphate, 0.05-0.1g/L of ferrous sulfate and 6.5-8.5 of pH.
The fermentation medium used in the invention comprises the following components: 12-16g/L yeast extract, 40-50g/L glucose, 50-60g/L sodium glutamate, 2-4g/L monopotassium phosphate, 0.5-2g/L ammonium citrate, 2-4g/L diammonium phosphate, 0.1-0.3g/L magnesium chloride, 0.05-0.1g/L manganese sulfate, 0.04-0.08g/L ferrous sulfate and pH of 7.5-8.5.
The components of the supplemented medium used by the invention are as follows: 12-16g/L of yeast extract, 50-60g/L of glucose, 50-60g/L of sodium glutamate, 0.1-0.3g/L of ferrous sulfate, 5-10g/L of diammonium phosphate and pH of 5.8-6.5.
When the method is used for fermentation production, the inoculation amount is 10-15%, the fermentation temperature is 38-39 ℃, the inoculation amount is 10-15%, the stirring rotating speed is 150-500rpm, the ventilation volume is 1.5-2vvm, and the tank pressure is 0.04-0.06 MPa. The pH value will decrease in the fermentation process, generally to the lowest point of 4.5-5.2, at this time, the pH value will not rise under the control of sulfuric acid (with concentration less than or equal to 30%), and the material is supplemented simultaneously in an intermittent manner, and is supplemented every 20-60min, and the amount of the supplemented material is controlled to be 1-3.5 ml/L/h.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to specific embodiments. But the invention is not limited thereto, and all the technologies realized based on the above-mentioned contents of the invention are within the scope of the invention
Example 1(50L fermenter)
Inoculating the preserved Bacillus subtilis strain into 300mL of first-stage seed culture medium, culturing in a shaker at 38 deg.C, rotating at 160rpm, and waiting for OD600At a value of 10, the cells were transferred to 3L of secondary seed medium and cultured in a shaker at 38 ℃ at 160rpm to OD600The value was 28, and the cells were inoculated into 30L of a fermentation medium (50L fermenter) under a pressure of 0.04MPa with a stirring speed of 500rpm and an aeration rate of 1.5vvm, while controlling the fermentation temperature at 38 ℃. Fermenting for 15h until pH of the fermentation liquid is reduced to 5.2The pH value of the fermentation liquor is automatically controlled to be maintained at 5.2 by 5% sulfuric acid, and meanwhile, the material is supplemented in a manner of supplementing the material once every 20min, wherein the material supplementing amount is 15mL each time. The fermentation time is 45h, and the content of polyglutamic acid in the fermentation liquor is 52g/L by sampling detection.
Wherein, the formula of the primary and secondary seed culture medium is as follows: 4g/L of yeast extract, 30g/L of glucose, 24g/L of sodium glutamate, 3g/L of monopotassium phosphate and 0.05g/L of ferrous sulfate, and the pH value is adjusted to 6.5. The formula of the fermentation medium is as follows: yeast extract 12g/L, glucose 40g/L, sodium glutamate 50g/L, potassium dihydrogen phosphate 2g/L, ammonium citrate 0.5g/L, diammonium phosphate 2g/L, magnesium chloride 0.1g/L, manganese sulfate 0.05g/L, ferrous sulfate 0.04g/L, adjusting pH to 7.5. The formula of the feed medium is as follows: yeast extract 12g/L, glucose 50g/L, sodium glutamate 50g/L, ferrous sulfate 0.1g/L, diammonium phosphate 5g/L, adjusting pH to 5.8.
Example 2(500L fermenter)
Inoculating the preserved Bacillus subtilis strain in 3L first-stage seed culture medium, culturing in shaking table at 39 deg.C, rotating at 160rpm, and waiting for OD600At a value of 15, the cells were transferred to a 30L secondary seed (50L fermenter) medium at a temperature of 39 ℃ and a stirring speed of 300rpm with an aeration rate of 2vvm and cultured to OD600The value was 36, and the cells were inoculated into 300L of a fermentation medium (500L fermenter) under conditions of a fermentation temperature of 39 ℃, a stirring speed of 400rpm, an aeration rate of 2vvm and a tank pressure of 0.06 MPa. Fermenting for 12h, reducing the pH value of the fermentation liquor to 4.5, automatically controlling the pH value of the fermentation liquor to be 4.5 by using 15% sulfuric acid, and simultaneously supplementing materials, wherein the supplementing materials are supplemented every 60min, and the supplementing amount is 1.05L each time. The fermentation time is 60h, and the content of polyglutamic acid in the fermentation liquor is 65g/L by sampling detection.
Wherein, the formula of the primary and secondary seed culture medium is as follows: yeast extract 12g/L, glucose 40g/L, sodium glutamate 33g/L, potassium dihydrogen phosphate 6g/L, ferrous sulfate 0.1g/L, adjusting pH to 8.5. The formula of the fermentation medium is as follows: 16g/L yeast extract, 50g/L glucose, 60g/L sodium glutamate, 4g/L potassium dihydrogen phosphate, 2g/L ammonium citrate, 4g/L diammonium phosphate, 0.3g/L magnesium chloride, 0.1g/L manganese sulfate and 0.08g/L ferrous sulfate, and the pH is adjusted to be 8.5. The formula of the feed medium is as follows: 16g/L of yeast extract, 60g/L of glucose, 60g/L of sodium glutamate, 0.3g/L of ferrous sulfate and 10g/L of diammonium phosphate, and the pH value is adjusted to 6.5.
Example 3(5 ton fermenter)
Inoculating Bacillus subtilis strain in 30L primary seed culture medium (50L fermentation tank), stirring at 38 deg.C and 500rpm, and allowing ventilation to reach 2vvm to OD600At a value of 12, the cells were transferred to a 300L secondary seed (500L fermenter) medium at a temperature of 39 ℃ and a stirring speed of 200rpm with an aeration rate of 1.5vvm and cultured to OD600The value was 33, and then inoculated into 3000L of a fermentation medium (5000L fermenter) under a pressure of 0.05MPa with a fermentation temperature of 38.5 ℃, a stirring rotation speed of 200rpm, an aeration amount of 2 vvm. Fermenting for 20h, reducing the pH value of the fermentation liquor to 4.6, automatically controlling the pH value of the fermentation liquor to be 4.6 by using 20% sulfuric acid, and simultaneously supplementing materials, wherein the supplementing materials are supplemented every 30min, and the supplementing amount is 4.5L each time. The fermentation time is 50h, and the content of polyglutamic acid in the fermentation liquor is 56.8g/L by sampling detection.
Wherein, the formula of the primary and secondary seed culture medium is as follows: 10g/L of yeast extract, 38g/L of glucose, 30g/L of sodium glutamate, 5g/L of monopotassium phosphate and 0.08g/L of ferrous sulfate, and the pH value is adjusted to 7.5. The formula of the fermentation medium is as follows: 15g/L of yeast extract, 48g/L of glucose, 55g/L of sodium glutamate, 3.5g/L of monopotassium phosphate, 1.6g/L of ammonium citrate, 3g/L of diammonium phosphate, 0.2g/L of magnesium chloride, 0.08g/L of manganese sulfate and 0.06g/L of ferrous sulfate, and the pH value is adjusted to be 8.0. The formula of the feed medium is as follows: yeast extract 15g/L, glucose 55g/L, sodium glutamate 57g/L, ferrous sulfate 0.2g/L, diammonium phosphate 6g/L, pH adjusted to 6.1.
Example 4(25 ton fermenter)
Inoculating Bacillus subtilis strain into 300L primary seed culture medium (500L fermentation tank), stirring at 39 deg.C and 300rpm, and allowing ventilation to be 1.8vvm until OD600At a value of 15, the cells were transferred to 2500L of secondary seed (5000L fermenter) medium at 38 ℃ with a stirring speed of 150rpm and an aeration rate of 2vvm and cultured to OD600The value was 36, and then inoculated into 17500L of fermentation medium (25000L fermenter) under conditions of fermentation temperature of 39 ℃, stirring speed of 150rpm, aeration rate of 2vvm, and tank pressure of 0.06 MPa. Fermenting for 18h to obtain fermentation liquorThe pH value is reduced to 4.8, the pH value of the fermentation liquor is automatically controlled to be maintained at 4.8 by using 30% sulfuric acid, and meanwhile, the materials are supplemented in a mode of supplementing materials once every 40min, and the supplementing amount is 35L each time. The fermentation time is 60h, and the content of polyglutamic acid in the fermentation liquor is 62.5g/L by sampling detection.
Wherein, the formula of the primary and secondary seed culture medium is as follows: 8.5g/L of yeast extract, 38.5g/L of glucose, 33g/L of sodium glutamate, 4g/L of monopotassium phosphate and 0.05g/L of ferrous sulfate, and the pH value is adjusted to 6.8. The formula of the fermentation medium is as follows: 16g/L of yeast extract, 47g/L of glucose, 60g/L of sodium glutamate, 4g/L of monopotassium phosphate, 1g/L of ammonium citrate, 2.5g/L of diammonium phosphate, 0.15g/L of magnesium chloride, 0.1g/L of manganese sulfate and 0.04g/L of ferrous sulfate, and the pH value is adjusted to be 8.2. The formula of the feed medium is as follows: yeast extract 15g/L, glucose 53g/L, sodium glutamate 58g/L, ferrous sulfate 0.1g/L, diammonium phosphate 6.5g/L, pH adjusted to 6.0.
While the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (8)
1. A fermentation method of high-content polyglutamic acid. The method is characterized in that: the fermentation process comprises the following steps: culturing the bacillus subtilis producing polyglutamic acid in a primary seed culture medium to OD600Value 10-15, transferring to secondary seed culture to OD600The pH value is 28-36, inoculating the fermentation medium, when the pH value of the fermentation liquid is lowered to the lowest point and begins to rise, dropwise adding sulfuric acid to control the pH value of the fermentation liquid to be kept at the point until the fermentation is finished, and simultaneously starting to perform intermittent feeding until the fermentation is finished, wherein the fermentation period is generally 45-60h, and the content of the polyglutamic acid can reach 50-65 g/L.
2. The method of claim 1, wherein the Bacillus subtilis group for producing polyglutamic acid is Shandong peptide and deposited strain of Biotech Ltd.
3. The method of claim 1, wherein the primary and secondary seed culture media comprise: 4-12g/L of yeast extract, 30-40g/L of glucose, 24-33g/L of sodium glutamate, 3-6g/L of monopotassium phosphate, 0.05-0.1g/L of ferrous sulfate and 6.5-8.5 of pH.
4. The method for fermenting polyglutamic acid according to claim 1, wherein the fermentation medium comprises: 12-16g/L yeast extract, 40-50g/L glucose, 50-60g/L sodium glutamate, 2-4g/L monopotassium phosphate, 0.5-2g/L ammonium citrate, 2-4g/L diammonium phosphate, 0.1-0.3g/L magnesium chloride, 0.05-0.1g/L manganese sulfate, 0.04-0.08g/L ferrous sulfate and pH of 7.5-8.5.
5. The fermentation method of polyglutamic acid according to claim 1, wherein the inoculation amount is 10-15%, the culture temperature is 38-39 ℃, the inoculation amount is 10-15%, the stirring rotation speed is 150-500rpm, the aeration amount is 1.5-2vvm, and the tank pressure is 0.04-0.06 Mpa.
6. The method for fermenting polyglutamic acid according to claim 1, wherein the feed medium comprises: 12-16g/L of yeast extract, 50-60g/L of glucose, 50-60g/L of sodium glutamate, 0.1-0.3g/L of ferrous sulfate, 5-10g/L of diammonium phosphate and pH of 5.8-6.5.
7. The method of claim 1, wherein the lowest pH of the fermentation broth is generally between 4.5 and 5.2, and the concentration of sulfuric acid used for pH control is 30% or less.
8. The fermentation method of polyglutamic acid of claim 1, wherein the intermittent feeding is performed every 20-60min, and the feeding is controlled to be 1-4 ml/L/h.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528422A (en) * | 2021-09-06 | 2021-10-22 | 丽江映华生物药业有限公司 | Culture method of polyglutamic acid seed liquid |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103881954A (en) * | 2012-12-20 | 2014-06-25 | 山东福瑞达生物科技有限公司 | Gamma-polyglutamic acid production gene engineering bacterial and method for producing high-yield gamma-polyglutamic acid through gamma-polyglutamic acid production gene engineering bacterial |
| CN104087628A (en) * | 2014-04-28 | 2014-10-08 | 广西大学 | Method for reducing viscosity of gamma-polyglutamic acid fermentation liquid |
| CN106636240A (en) * | 2016-11-17 | 2017-05-10 | 东莞波顿香料有限公司 | High-concentration gamma-polyglutamic acid and fermentation method thereof |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103881954A (en) * | 2012-12-20 | 2014-06-25 | 山东福瑞达生物科技有限公司 | Gamma-polyglutamic acid production gene engineering bacterial and method for producing high-yield gamma-polyglutamic acid through gamma-polyglutamic acid production gene engineering bacterial |
| CN104087628A (en) * | 2014-04-28 | 2014-10-08 | 广西大学 | Method for reducing viscosity of gamma-polyglutamic acid fermentation liquid |
| CN106636240A (en) * | 2016-11-17 | 2017-05-10 | 东莞波顿香料有限公司 | High-concentration gamma-polyglutamic acid and fermentation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| QUN WU等: "Kinetic analysis and pH-shift control strategy for poly(γ-glutamic acid) production with Bacillus subtilis CGMCC 0833", 《BIOCHEMICAL ENGINEERING JOURNAL》 * |
| 惠明等: "pH值对聚谷氨酸发酵液粘度及聚合物结构的影响", 《食品与发酵工业》 * |
| 马晓娜等: "一株产絮凝剂芽孢杆菌的分离及成份分析", 《微生物学通报》 * |
| 高华等: "高分子量聚谷氨酸的微生物合成及其发酵过程的研究", 《现代生物医学进展》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528422A (en) * | 2021-09-06 | 2021-10-22 | 丽江映华生物药业有限公司 | Culture method of polyglutamic acid seed liquid |
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