CN111118082A - Method for recycling potato starch wastewater - Google Patents
Method for recycling potato starch wastewater Download PDFInfo
- Publication number
- CN111118082A CN111118082A CN202010106834.6A CN202010106834A CN111118082A CN 111118082 A CN111118082 A CN 111118082A CN 202010106834 A CN202010106834 A CN 202010106834A CN 111118082 A CN111118082 A CN 111118082A
- Authority
- CN
- China
- Prior art keywords
- potato starch
- culture medium
- fermentation
- starch wastewater
- recycling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for preparing bacterial cellulose by processing and separating juice from potato starch. According to the invention, potato starch processing and separating juice is used as a culture medium raw material, a carbon source, a nitrogen source and inorganic salt are compounded according to the chemical composition category and content in wastewater in a certain proportion, the pH value of the culture medium is adjusted after sterilization and cooling, then acetobacter gluconicum in a certain proportion is inoculated, and the bacterial cellulose is prepared by fermentation in a dynamic or static manner. The method creatively uses the potato starch processing and separating juice as the culture medium to prepare the bacterial cellulose, the bacterial cellulose is a safe and healthy high-fiber raw material and has better application value, and the potato starch processing and separating juice is wastewater with higher processing difficulty and cost in the potato starch processing.
Description
Technical Field
The invention belongs to the technical field of food biology, and particularly relates to a method for recycling potato starch wastewater.
Background
Fresh potatoes contain about 17-21% starch, 0.5-1.2% protein, and are therefore often used for starch production. The wastewater in the potato starch processing process mainly comes from three parts of raw material washing wastewater, potato starch processing separation juice and potato starch washing water, and according to statistics, 5-12 m can be generated when 1t of potato starch is produced3And (4) waste liquor. Wherein the potato starch is processedThe juice separating water is a main pollutant of the potato starch processing wastewater, the partial wastewater mainly contains a small amount of starch, protein, amino acid, pectin, sugar and mineral salt, the protein content is 1.5-2.8%, the protein accounts for about 30-40% of the total amount of the wastewater, and the COD value is as high as 40000-60000 mg/L. With the issuance and implementation of the potato staple food strategy, through the continuous adjustment of the potato starch industry and the continuous introduction of advanced technology, the potato processing industry in China will be rapidly developed, and then a large amount of potato starch wastewater with high concentration, serious pollution and difficult treatment will be generated, and if a solution cannot be found, the development of the potato starch processing industry in China will be severely restricted. Therefore, the treatment of the potato starch processing wastewater becomes a bottleneck problem restricting the development of the potato processing industry.
The conventional starch wastewater treatment method is mainly divided into a biological method, a physical method and a chemical oxidation method, various non-edible additives are added in the conventional wastewater treatment method, the conventional wastewater treatment method can only be used for treating solid waste residues, and the increment rate is low. The literature reports that the high-value utilization approach of potato starch processing and juice separation mainly has two aspects, one is that the effective components in the potato starch processing and juice separation are converted into single-cell protein by a method of microbial fermentation engineering and the single-cell protein is used as animal feed; and secondly, beneficial substances are separated and extracted, the available carbon source comprises pectin, fiber, biomass fuel and other trace beneficial components, the available nitrogen source comprises protein products obtained by an enzymatic hydrolysis treatment mode and animal feed obtained by fermentation, and the extraction rate of the protein in the waste water produced by separating and extracting the potato starch reaches over 90 percent.
The bacterial cellulose is prepared by fermenting gluconacetobacter xylinus under the condition of a culture medium containing a nitrogen source, a carbon source and necessary growth factors, has good water holding performance, higher crystallinity and polymerization degree and good biocompatibility and biodegradability, and can be widely used in various fields of food, medicine, aviation, paper making and the like. The traditional fermentation production of bacterial cellulose adopts a by-product coconut water or coconut milk of an agricultural product coconut as a raw material, and the raw material is not storage-resistant and is easy to decay and deteriorate. In order to ensure the quality of the bacterial cellulose, fresh coconut water or coconut milk raw materials need to be collected again every time fermentation production is carried out, so that the coconut resources of agricultural products are greatly wasted. In addition, as the application field of bacterial cellulose is continuously expanded, the preparation of bacterial cellulose by only using coconut water as a fermentation medium cannot meet the demand. The prior documents report a plurality of culture mediums for fermentation, such as the preparation of bacterial cellulose by using soybean dregs, cassava hydrolysate, whey or rice soaking water as culture medium raw materials.
According to the invention, according to the properties of the bacterial cellulose production raw material, the potato starch processing and separating juice in the potato starch processing wastewater is selected as the raw material in a targeted manner, and the potato starch processing and separating juice is creatively recycled by adopting a microbial fermentation engineering method and is converted into a high value-added product. The implementation of the invention provides a feasible resource effective utilization way for potato starch processing enterprises, and can promote the sustainable development of the enterprises at the same time.
Disclosure of Invention
The invention provides a method for recycling potato starch wastewater, which comprises the following steps:
(1) selecting a culture medium, namely separating juice produced in the process of processing and separating potato residue and starch from potato starch, wherein the partial separating juice mainly contains starch, protein, amino acid, pectin, saccharides and mineral substances; the COD value of the culture medium is more than 20000 mg/L.
(2) Standardizing the culture medium in the step (1), wherein the content of each component of the treated culture medium is as follows: 88 to 90 percent of water, 4 to 8 percent of total sugar, 2 to 4 percent of protein and 0.5 to 3 percent of inorganic salt; the specific treatment method is to add one or more reducing sugars such as sucrose, high fructose syrup, glucose and the like into the culture medium, wherein the reducing sugars comprise one or more nitrogen sources of common culture media such as peptone, beef extract and the like or one or more plant proteins such as soybean protein, pea protein, rice protein and the like.
(3) Sterilizing the treated culture medium at high pressure, and cooling for later use;
(4) adjusting the pH value to 2.5-3.5 by using one or more of acetic acid, citric acid or lactic acid;
(5) adding 0.05-0.1% (based on the content of solid in the solution) of gluconacetobacter xylinus into the culture medium, and fermenting in a constant-temperature constant-humidity incubator with the temperature of 30-37 ℃ and the humidity of 60-85%; static fermentation or dynamic fermentation may be used. The static fermentation time is 36-60 h; the fermentation time in the dynamic fermentation process is 12-48 h, and the stirring speed is 50-100 r/min.
(6) And (4) obtaining bacterial cellulose after fermentation is finished, washing the residual fermentation liquor on the surface by using water, and storing for later use.
The cellulose obtained by adopting static fermentation is in a whole sheet shape, and the fiber nano-filaments are compact in shape and can be used for masks, paper and the like; the cellulose obtained by dynamic fermentation is in a small cluster form and is used for beverages, adsorbing materials and the like.
The invention has the advantages of
(1) The separated juice in the potato starch processing wastewater is rich in nutrient components, so that the potato starch processing wastewater is easy to decay and deteriorate in the untimely treatment and storage process, the treatment cost of the part of the wastewater is high, the technical difficulty is high, and most enterprises directly discharge the part of the wastewater, so that the protein rich in nutrition in the potato wastewater is lost, the environment is polluted, and the ecology is influenced. The invention can recycle and directly utilize the part of wastewater, reduces the risk of putrefaction and deterioration in the storage process of the part of wastewater, reduces sewage discharge and improves the reutilization of resources.
(2) The fermentation medium has a low pH value, can effectively inhibit the growth of other putrefying bacteria under the action of the dominant bacteria of the gluconacetobacter xylinum, improves the controllability of the production process of enterprises, and has strong technical application and popularization.
(3) The bacterial cellulose prepared by the method has good water holding performance, higher crystallinity and polymerization degree, and good biocompatibility and biodegradability, is a high-quality biological material, can be widely used in various fields of food, medicine, aviation, paper making and the like, and provides a feasible way for driving local economic development.
Description of the drawings:
FIG. 1 shows bacterial cellulose prepared by static fermentation;
FIG. 2 shows bacterial cellulose prepared by dynamic fermentation.
Detailed Description
Selecting a potato starch processing and separating juice sample, and determining the chemical composition of the potato starch processing and separating juice sample as follows: the present invention will be described in more detail with reference to specific embodiments using 93% water, 1.8% protein, 1.4% total sugar and a COD value of 23000mg/L as raw materials.
(1) Mode of static fermentation
Taking the wastewater as a raw material, supplementing and adding saccharides and proteins, taking the adjusted culture medium containing 88% of water, 8% of total sugar and 2% of proteins as a standard culture medium, carrying out high-pressure sterilization on the standard culture medium, cooling, adjusting the pH value of the culture medium to be 3.5, adding 0.1% (based on the content of solid matters in the solution) of acetobacter gluconicum into the acid-adjusted culture medium, carrying out static fermentation for 36h in a constant-temperature constant-humidity culture box with the temperature of 37 ℃ and the humidity of 85%, taking out bacterial cellulose after the fermentation is finished, washing off the residual fermentation liquor on the surface by water, and storing for later use.
Taking the wastewater as a raw material, supplementing and adding saccharides and proteins, taking the adjusted culture medium containing 89% of water, 6% of total sugar and 3% of proteins as a standard culture medium, carrying out high-pressure sterilization on the standard culture medium, cooling, adjusting the pH value of the culture medium to be 3, adding 0.08% (based on the content of solid matters in the solution) of acetobacter gluconicum into the acid-adjusted culture medium, carrying out static fermentation for 48h in a constant-temperature constant-humidity incubator at the temperature of 33 ℃ and the humidity of 75%, taking out bacterial cellulose after the fermentation is finished, washing off residual fermentation liquor on the surface by using water, and storing for later use.
Taking the wastewater as a raw material, supplementing and adding saccharides and proteins, taking the adjusted culture medium containing 90% of water, 4% of total sugar and 4% of proteins as a standard culture medium, carrying out high-pressure sterilization on the standard culture medium, cooling, adjusting the pH value of the culture medium to be 2.5, adding 0.05% (based on the content of solid matters in the solution) of acetobacter gluconicum into the acid-adjusted culture medium, carrying out static fermentation for 60h in a constant-temperature constant-humidity culture box with the temperature of 30 ℃ and the humidity of 60%, taking out bacterial cellulose after the fermentation is finished, washing off the residual fermentation liquor on the surface by water, and storing for later use.
The picture of the appearance of the bacterial cellulose prepared by the above examples 1-3 is shown in fig. 1, and it can be seen from the figure that: the bacterial cellulose prepared by the static fermentation mode is in a whole sheet shape, the fiber nano-filaments are compact in shape, the water content is 85% -92% through determination, the cellulose dry basis content is 97% -99%, and the bacterial cellulose is mainly used for masks, paper and the like.
(2) Mode of dynamic fermentation
Taking the wastewater as a raw material, supplementing and adding saccharides and proteins, taking the adjusted culture medium containing 88% of water, 8% of total sugar and 2% of proteins as a standard culture medium, carrying out high-pressure sterilization on the standard culture medium, cooling, adjusting the pH value of the culture medium to be 3.5, adding 0.1% (based on the content of solid matters in the solution) of acetobacter gluconicum into the acid-adjusted culture medium, fermenting for 12 hours in a shaking table incubator with the temperature of 37 ℃ and the stirring speed of 100r/min, taking out bacterial cellulose after the fermentation is finished, washing away the residual fermentation liquor on the surface by water, and storing for later use.
Taking the wastewater as a raw material, supplementing and adding saccharides and proteins, taking the adjusted culture medium containing 89% of water, 6% of total sugar and 3% of proteins as a standard culture medium, carrying out high-pressure sterilization on the standard culture medium, cooling, adjusting the pH value of the culture medium to be 3, adding 0.08% (based on the content of solid matters in the solution) of acetobacter gluconicum into the acid-adjusted culture medium, fermenting for 30 hours in a shaking table incubator at the temperature of 33 ℃ and the stirring rate of 75r/min, taking out bacterial cellulose after the fermentation is finished, washing off residual fermentation liquor on the surface by water, and storing for later use.
Taking the wastewater as a raw material, supplementing and adding saccharides and proteins, taking the adjusted culture medium containing 90% of water, 4% of total sugar and 4% of proteins as a standard culture medium, carrying out high-pressure sterilization on the standard culture medium, cooling, adjusting the pH value of the culture medium to be 2.5, adding 0.05% (based on the content of solid matters in the solution) of acetobacter gluconicum into the acid-adjusted culture medium, fermenting for 48 hours in a shaking table incubator with the temperature of 30 ℃ and the stirring speed of 50r/min, taking out bacterial cellulose after the fermentation is finished, washing away the residual fermentation liquor on the surface by water, and storing for later use.
The picture of the appearance of the bacterial cellulose prepared by the above examples 4-6 is shown in fig. 2, and it can be seen from the picture that: the bacterial cellulose prepared by the dynamic fermentation mode is in a small-cluster shape, has the water content of 90-99 percent and the cellulose dry basis content of 95-97 percent, and is mainly used for beverages, adsorbing materials and the like.
The potato starch processing and separating juice is recycled by a microbial fermentation engineering method, and a bacterial cellulose product with high added value is prepared. The amount of wastewater before and after treatment and its COD value are shown in Table 1, and the comparison of the data in the table can give: the invention effectively reduces the COD value in the wastewater by utilizing a microbial fermentation mode, and reduces the difficulty of wastewater treatment; due to the fact that the prepared bacterial cellulose has high water binding capacity, the amount of wastewater to be treated after final conversion is remarkably reduced.
TABLE 1 values of the parameters of the wastewater before and after treatment
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (8)
1. A method for recycling potato starch wastewater is characterized by comprising the following steps:
(1) selecting a culture medium, namely separating juice produced in the process of processing and separating potato residue and starch from potato starch;
(2) standardizing the culture medium in the step (1), wherein the content of each component of the treated culture medium is as follows: 88-90% of water, 4-8% of total sugar, 2-4% of protein and the balance of inorganic salt;
(3) sterilizing the treated culture medium at high pressure, and cooling for later use;
(4) adjusting the pH value of the cooled culture medium to 2.5-3.5;
(5) adding 0.05-0.1% (based on the content of solid matters in the solution) of acetobacter gluconicum into the culture medium, and fermenting in a constant-temperature constant-humidity culture box with the temperature of 30-37 ℃ and the humidity of 60-85%;
(6) and (4) obtaining bacterial cellulose after fermentation is finished, washing the residual fermentation liquor on the surface by using water, and storing for later use.
2. The method for recycling potato starch wastewater according to claim 1, wherein the COD value of the culture medium in the step (1) is more than 20000 mg/L.
3. The method for recycling potato starch wastewater as claimed in claim 1, wherein when the required chemical components and contents of the culture medium are different from those in step (2), the step (2) is supplemented with sugar and protein to reach the content requirement.
4. The method for recycling potato starch wastewater as claimed in claim 3, wherein the added sugar comprises one or more of sucrose, high fructose syrup, glucose and other reducing sugars; the protein comprises common culture medium nitrogen sources such as peptone, beef extract, etc., or one or more of vegetable proteins such as soybean protein, pea protein, rice protein, etc.
5. The method for recycling potato starch wastewater as claimed in claim 1, wherein the pH value of the potato starch wastewater in the step (4) is adjusted by one or more of acetic acid, citric acid or lactic acid.
6. The method for recycling potato starch wastewater according to claim 1, wherein the fermentation time in the step (5) is 12-60 h.
7. The method for recycling potato starch wastewater according to claim 1, wherein the fermentation mode adopted in the step (5) is static fermentation, and the static fermentation time is 36-60 h.
8. The method for recycling potato starch wastewater according to claim 1, wherein the fermentation mode adopted in the step (5) is dynamic fermentation, the fermentation time in the dynamic fermentation process is 12-48 h, and the stirring speed is 50-100 r/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010106834.6A CN111118082B (en) | 2020-03-30 | 2020-03-30 | Method for recycling potato starch wastewater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010106834.6A CN111118082B (en) | 2020-03-30 | 2020-03-30 | Method for recycling potato starch wastewater |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111118082A true CN111118082A (en) | 2020-05-08 |
| CN111118082B CN111118082B (en) | 2022-12-16 |
Family
ID=70492543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010106834.6A Active CN111118082B (en) | 2020-03-30 | 2020-03-30 | Method for recycling potato starch wastewater |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111118082B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2815933C1 (en) * | 2023-06-06 | 2024-03-25 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Российский химико-технологический университет имени Д.И. Менделеева" (РХТУ им. Д.И. Менделеева) | Method of producing lactic acid from by-products of starch production when processing wheat grains |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210383A (en) * | 2011-04-08 | 2011-10-12 | 甘肃圣大方舟马铃薯变性淀粉有限公司 | Method for preparing mycoprotein feed from waste water and waste residues in processing process of potatoes |
| CN102703543A (en) * | 2012-06-25 | 2012-10-03 | 东华大学 | Method for preparing bacterial cellulose by tuberous raw materials |
-
2020
- 2020-03-30 CN CN202010106834.6A patent/CN111118082B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210383A (en) * | 2011-04-08 | 2011-10-12 | 甘肃圣大方舟马铃薯变性淀粉有限公司 | Method for preparing mycoprotein feed from waste water and waste residues in processing process of potatoes |
| CN102703543A (en) * | 2012-06-25 | 2012-10-03 | 东华大学 | Method for preparing bacterial cellulose by tuberous raw materials |
Non-Patent Citations (1)
| Title |
|---|
| MARZENA JEDRZEJCZAK-KRZEPKOWSKA等: "《Bacterial Nanocellulose From Biotechnology to Bio-Economy》", 22 July 2016 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2815933C1 (en) * | 2023-06-06 | 2024-03-25 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Российский химико-технологический университет имени Д.И. Менделеева" (РХТУ им. Д.И. Менделеева) | Method of producing lactic acid from by-products of starch production when processing wheat grains |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111118082B (en) | 2022-12-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jagannath et al. | The effect of pH, sucrose and ammonium sulphate concentrations on the production of bacterial cellulose (Nata-de-coco) by Acetobacter xylinum | |
| Velásquez-Riaño et al. | Production of bacterial cellulose from alternative low-cost substrates | |
| Lestari et al. | Study on the production of bacterial cellulose from Acetobacter xylinum using agro-waste | |
| Jozala et al. | Bacterial cellulose production by Gluconacetobacter xylinus by employing alternative culture media | |
| Kongruang | Bacterial cellulose production by Acetobacter xylinum strains from agricultural waste products | |
| Oumer et al. | Comparative studies of pectinase production by Bacillus subtilis strain Btk 27 in submerged and solid-state fermentations | |
| Ahmed et al. | Production of microbial biomass protein by sequential culture fermentation of Arachniotus sp. and Candida utilis | |
| US9994872B2 (en) | Integration of first and second generation bioethanol processes | |
| CN1043659C (en) | Fermentation process for producing natamycin | |
| CN112205514B (en) | Method for producing multifunctional distiller's grains feed | |
| CN107535671A (en) | Improve the microbial fermentation yellow wine lees feed and preparation method of ruminal protein utilization rate | |
| Corujo et al. | Production of bacterial nanocellulose from non-conventional fermentation media | |
| CN1038835A (en) | The production method of persimmon vinegar | |
| Ray et al. | Bio-ethanol, bioplastics and other fermented industrial products from cassava starch and flour | |
| Azam et al. | Date palm waste: an efficient source for production of glucose and lactic acid | |
| CN111118082B (en) | Method for recycling potato starch wastewater | |
| CN1810734A (en) | Refuse composting process adding different decay promoting ferments separately and its decay promoting ferments | |
| Abdel-Azeem et al. | Bioconversion of lignocellulosic residues into single-cell protein (SCP) by Chaetomium | |
| WO2017144389A1 (en) | Process for propagating a yeast capable to ferment glucose and xylose | |
| CN102911982B (en) | Fructus Musae purposes in Biological cellulose fermentation culture medium | |
| US9970016B2 (en) | Genetic engineered bacteria and methods for promoting production of succinic acid or lactic acid | |
| US6132998A (en) | Process for continuously preparing bacterial cellulose | |
| CN114574527B (en) | Fermentation method for optimizing hydrolyzed feather meal | |
| Özkaya et al. | Utilization of food waste in production of bacterial cellulose | |
| Ellamar et al. | Bioconversion of Lignocellulosic Agricultural By-Products by Microorganisms into High Mycoprotein Feeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |