CN111117932A - Alcaligenes faecalis and application thereof in degradation of ethylene oxide - Google Patents
Alcaligenes faecalis and application thereof in degradation of ethylene oxide Download PDFInfo
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Abstract
The invention discloses an alcaligenes faecalis and application thereof, wherein the strain is preserved in the China general microbiological culture Collection center of the culture Collection management Committee (CGMCC No. 18435) in 2019, 8 and 29 months, and the preservation address is the institute of microbiology of China academy of sciences No.3, Navy, Naja, Beijing; the taxonomy of the strain is named Alcaligenes faecalis (Alcaligenes faecalis) and is named EO-05; the strain is applied to industrial degradation of ethylene oxide, and the harmless treatment capacity of ethylene oxide is improved.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to alcaligenes faecalis and application thereof in degradation of ethylene oxide.
Background
Ethylene oxide is one of the important petrochemical products in the field of modern chemical industry. Because the ethylene oxide has high penetrability and can generate coupling chemical reaction with biological macromolecules, the ethylene oxide has an important position in the medical sterilization industry. In addition, ethylene oxide sterilization is one of the most important low-temperature sterilization agents so far, because it is low in cost and can be used for industrial-scale sterilization treatment. A large amount of ethylene oxide remains in the sewage of petrochemical industry, but because the ethylene oxide is extremely active, inflammable and explosive, and is a recognized carcinogen in the world, the ethylene oxide needs to be degraded.
At present, the harmless treatment method of ethylene oxide in China mainly utilizes sulfuric acid to carry out oxidation reaction on the ethylene oxide, and has the defect that a large amount of industrial wastewater containing the ethylene oxide can be discharged after chemical treatment, so that the real harmless requirement cannot be met.
However, currently, research on effective strains for degrading ethylene oxide is less, and the harmless treatment capability of ethylene oxide can be improved only by effectively screening out microorganisms for degrading ethylene oxide.
Therefore, how to provide a strain capable of degrading ethylene oxide is a problem that needs to be solved urgently by those skilled in the art.
Disclosure of Invention
In view of the above, the invention provides an alcaligenes faecalis capable of degrading ethylene oxide, and improves the harmless treatment capacity to ethylene oxide in industrial production.
In order to achieve the purpose, the invention adopts the following technical scheme:
an alcaligenes faecalis, wherein the strain is preserved in China general microbiological culture Collection center (CGMCC) on 29.8.2019, the preservation number is CGMCC No.18435, and the preservation address is the institute of microbiology, China academy of sciences, No.3, North West Lu No.1 Hospital, Chaozhou, Chaoyang, City; the taxonomy of the strain is named Alcaligenes faecalis (Alcaligenes faecalis) and is named EO-05.
The technical effect achieved by the technical scheme is as follows: firstly, collecting soil, silt and sewage samples at a sewage discharge port of a sewage treatment plant or a chemical plant, screening out ethylene oxide degradation potential bacteria through low-concentration ethylene oxide tolerance enrichment and purification, then sequentially subculturing and scribing the screened ethylene oxide degradation potential bacteria on ethylene oxide tolerance domestication culture medium plates containing different ethylene oxide concentrations (100mg/L-800mg/L), placing the plates on a 37 ℃ incubator for culturing for 24-48h, and finally selecting a single bacterial colony with the largest bacterial colony radius on the ethylene oxide tolerance screening plate containing 500 plus materials 800mg/L to obtain the ethylene oxide tolerance bacteria; the ethylene oxide-tolerant bacteria are sequentially subcultured and streaked on a culture medium which contains 500-800mg/L ethylene oxide and contains carbon sources (50%, 30%, 10%, 0%) in different proportions to perform domestication of ethylene oxide degradation capability. Selecting a single colony with the largest colony radius for preservation on a 500-800mg/L and 0% carbon source ethylene oxide degradation domestication culture medium plate to obtain a dominant strain EO-05 with ethylene oxide degradation potential, and preserving by adopting a glycerol preservation method (the culture solution and 50% glycerol are preserved at the temperature of-80 ℃); the strain is subjected to ethylene oxide degradation tests, and the ethylene oxide degradation capability is high, and the degradation rate can reach 68.5% under the condition of no carbon source.
An application of alcaligenes faecalis in degrading epoxy ethane.
The technical effect achieved by the technical scheme is as follows: the domesticated alcaligenes faecalis EO-05 has strong capability of degrading ethylene oxide, and the degradation rate can reach 68.5 percent, so the alcaligenes faecalis EO-05 can be applied to the treatment of industrial sewage, and the harm of the ethylene oxide is effectively eliminated.
According to the technical scheme, compared with the prior art, the invention discloses and provides the alcaligenes faecalis capable of degrading the ethylene oxide, and the harmless treatment capacity to the ethylene oxide in industrial production is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a diagram showing the colony growth of an oxirane-degrading potential bacterium;
FIG. 2 is a graph showing the results of gram staining potential bacteria for ethylene oxide degradation;
FIG. 3 is a phylogenetic evolution diagram of potential bacteria for ethylene oxide degradation;
FIG. 4 is a diagram showing the growth of EO-04 strain in a medium containing 800mg/L ethylene oxide and 0 carbon source;
FIG. 5 is a graph showing the growth of potential bacteria for ethylene oxide degradation in a culture medium containing 800mg/L ethylene oxide and 0 carbon source.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
EXAMPLE 1 screening of potential ethylene oxide-degrading bacteria
The sample of this example was taken from the sewage sludge mixture of suburb sewage treatment plant in Guangzhou, Guangdong province:
1) weighing 10.0g of sample sludge mixture, adding 100mL of 0.03mol/L phosphate buffer solution, uniformly mixing, clarifying for 120min, and filtering to remove large-particle silt to obtain suspension;
2) adding 1mL of suspension into 4 test tubes containing 10mL of liquid enrichment culture medium (tryptone soybean broth culture medium containing 100mg/L ethylene oxide), placing in a shaking table for oxygen-consuming enrichment culture at the rotation speed of 200r/min and culturing at 37 ℃ for 24-48 h;
3) and (3) carrying out streak separation on the dominant strains in the liquid enrichment culture medium on a screening culture medium to separate out the ethylene oxide degradation potential bacteria. Selecting single colony, culturing in enrichment broth without ethylene oxide for 24 hr, and preserving by glycerol preservation (culture solution and 50% glycerol at ratio of 1:1 and-80 deg.C).
The liquid enrichment medium comprises the following components: 17g of tryptone, 3g of soybean peptone, 5g of sodium chloride, 2.5g of dipotassium phosphate, 2.5g of glucose, 7.4 of pH value and 1000mL of distilled water are subpackaged into 500mL conical bottles, 250mL of each conical bottle is subpackaged, sterilized at 121 ℃ for 20min, cooled to room temperature, an ice box is taken out of a refrigerator, and a reagent of pure ethylene oxide is placed on the ice box. mu.L of ethylene oxide liquid was aspirated by a sealed syringe and injected into the sterilized medium (ethylene oxide concentration in the medium was 100mg/L, which meets the national discharge standards).
In this example, selection medium was composed of tryptone (17 g), soybean peptone (3 g), sodium chloride (5 g), dipotassium hydrogen phosphate (2.5 g), glucose (2.5 g), agar (15 g), pH 7.4 and distilled water (1000 mL). The screening medium was prepared and dispensed into 500mL Erlenmeyer flasks, 250mL each. Sterilizing at 121 deg.C for 20min, and when the temperature of the culture medium is reduced to about 50-56 deg.C, sucking 28 μ L ethylene oxide liquid with a sealed syringe, injecting into the sterilized culture medium, and making into culture plate.
According to the scheme, a single colony is obtained on a screening culture plate through a plate marking method, and a strain of ethylene oxide degradation potential bacteria is separated. When the bacterial colony is cultured for 48 hours, the bacterial colony is characterized by gray-white bacterial colony, irregular edge and spreading growth, the diameter of the bacterial colony is 4.0-6.0mm, and blue-green fluorescent pigment exists, and the result is detailed as shown in figure 1;
example 2 identification
The strain identification method comprises the following steps:
a. morphological feature identification: observing colony morphology, observing thallus microscopic morphology, and identifying culture characteristics and gram staining;
b. and (3) physiological and biochemical characteristic identification: the physical and chemical properties comprise nutrition types, nitrogen and carbon source utilization capacity, biochemical tests and the like;
c. molecular biological identification: sequentially carrying out thallus culture, bacterial DNA extraction, PCR amplification, 16s gene sequencing and sequence comparison analysis;
the upstream primer 27F: 5'-AGAGTTTGATCCTGGCTCAG-3', as shown in SEQ ID NO. 1;
a downstream primer 1492R: 5'-GGTTACCTTGTTACGACTT-3', as shown in SEQ ID NO. 2;
the gene sequencing result is shown in SEQ ID NO. 3; the 16S rRNA sequence is subjected to nucleotide sequence BLAST comparison, and the homology with 16S rRNA of Alcaligenes faecalis (Alcaligenes faecalis) is 99 percent;
the oxirane degradation potential bacteria colony is characterized by offwhite colony, irregular edge, spread growth, colony diameter of 4.0-6.0mm and blue-green fluorescent pigment. Microscopic observation shows that the thalli are short rods or spheres, are singly arranged, do not produce spores, are negative in gram stain and show a staining result in FIG. 2; the phylogenetic tree of this strain is shown in FIG. 3;
the screened and purified ethylene oxide degradation potential bacteria are bacteria of Alcaligenes faecalis (Alcaligenes faecalis) and Alcaligenes (Alcaligenes) through morphological characteristic identification, physiological and biochemical characteristic identification and molecular biological identification of the strains.
Example 3 Induction of acclimatization
Stage one: and (3) ethylene oxide tolerance induction domestication: inoculating the ethylene oxide degradation potential bacteria into a tolerant domestication culture medium containing 100mg/L ethylene oxide by using a plate streaking method, and culturing at the constant temperature of 37 ℃ for 48 h; selecting a single colony with the largest colony radius on the plate, subculturing the single colony into a tolerance domestication culture medium containing 200mg/L ethylene oxide, and culturing the single colony at the constant temperature of 37 ℃ for 48 hours; selecting a single colony with the largest colony radius on the plate, subculturing the single colony into a tolerance domestication culture medium containing 500mg/L ethylene oxide, and culturing the single colony at the constant temperature of 37 ℃ for 48 hours; selecting a single colony with the largest colony radius on the plate, subculturing the single colony into an ethylene oxide tolerance domestication culture medium containing 800mg/L, culturing the single colony at a constant temperature of 37 ℃ for 48 hours, and selecting the single colony with the largest colony radius on the plate to obtain a high-concentration ethylene oxide tolerance strain;
wherein the acclimation-tolerant culture medium comprises: 10g of peptone, 5g of beef extract, 5g of sodium chloride, 15g of agar, pH7.0-7.5 and 1000mL of distilled water, subpackaging into 250mL of standard, sterilizing at 121 ℃ for 20min, heating and melting the culture medium before use, adding 25mg, 50mg, 125mg and 200mg of ethylene oxide into a sealed injector respectively when the temperature of the culture medium is reduced to about 50-56 ℃, and preparing into four culture medium flat plates with different ethylene oxide concentrations (100mg/L, 200mg/L, 500mg/L and 800mg/L)
And a second stage: domestication of ethylene oxide degradation capacity: inoculating the ethylene oxide-tolerant bacteria into a degradation domestication culture medium containing 800mg/L ethylene oxide and 50% of a carbon source by using a plate streaking method, and culturing at a constant temperature of 37 ℃ for 48 h; selecting a single colony with the largest colony radius on the plate, subculturing the single colony into a degradation domestication culture medium containing 800 mg/ethylene oxide and 30% of carbon source, and culturing the single colony at a constant temperature of 37 ℃ for 48 hours; selecting a single colony with the largest colony radius on the plate, subculturing the single colony into a degradation domestication culture medium containing 800mg/L ethylene oxide and 10% of carbon source, and culturing the single colony at a constant temperature of 37 ℃ for 48 hours; selecting a single colony with the largest colony radius on the plate, subculturing the single colony into a degradation domestication culture medium containing 800mg/L ethylene oxide and 0% of carbon source, and culturing the single colony at the constant temperature of 37 ℃ for 48 hours; selecting a single colony with the largest radius on a degradation domestication culture medium containing 800mg/L of ethylene oxide and 0% of a carbon source; preserving the strain on the slant made of agar culture medium with corresponding nutrient components. The strain for degrading high-concentration ethylene oxide, namely the EO-05 strain is obtained and preserved by adopting a glycerol preservation method (the ratio of culture solution to 50 percent of glycerol).
Degrading and domesticating the culture medium:
1) 10g of peptone, 2.5g of beef extract, 5g of sodium chloride, 15g of agar, pH7.0-7.5 and 1000mL of distilled water.
2) 10g of peptone, 1.5g of beef extract, 5g of sodium chloride, 15g of agar, pH7.0-7.5 and 1000mL of distilled water.
3) 10g of peptone, 0.5g of beef extract, 5g of sodium chloride, 15g of agar, pH7.0-7.5 and 1000mL of distilled water.
4) 10g of peptone, 0g of beef extract, 5g of sodium chloride, 15g of agar, pH7.0-7.5 and 1000mL of distilled water.
Packaging the culture medium into 250ml, sterilizing at 121 deg.C for 20min, heating to melt the culture medium before use, and adding 200mg ethylene oxide into sealed injector when the temperature of the culture medium is reduced to about 50-56 deg.C to obtain four culture medium plates containing different carbon sources (50%, 30%, 10%, 0%) and 800mg/L ethylene oxide.
The results of the induction acclimatization of the two strains in the first stage and the second stage are shown in table 1;
TABLE 1
As shown in the table above, the culture conditions of the dominant species of ethylene oxide were gradually controlled to obtain a strain EO-05 having ethylene oxide-degrading ability. This result shows that the EO-05 strain can grow normally under the culture conditions in which ethylene oxide is the sole carbon source, and can utilize ethylene oxide as the carbon source.
The strain is preserved in China general microbiological culture Collection center (CGMCC) No.18435 at 29.8.2019, with the preservation number of CGMCC No.18435 and the preservation address of the institute of microbiology, China academy of sciences, No.3, West Lu 1, North Chen, Indor, Beijing.
The 16s rRNA sequence of the strain EO-05 acclimatized in example 3 was sequenced, and the sequencing result is shown in SEQ ID NO. 4.
Example 4 identification of ethylene oxide degrading Effect of EO-05 Strain
And (3) microbial culture activation: EO-05 strain was taken out from a freezer at-80 ℃ and 10. mu.L of the strain was inoculated into 100mL of tryptone soybean broth culture medium and cultured at 37 ℃ and 200rpm for 48 hours. The number of thalli in the culture bacterial liquid is 1010-1012cfu/mL。
Test groups:
test group 1: the pure culture strain EO-05 was inoculated into a liquid medium containing no carbon source but containing an ethylene oxide inducer at a concentration of 800mg/L in such a manner that 5mL of the cells were taken as 10 viable cells10-1012cfu/mL EO-05 bacteria culture medium to 400mL of nutrient broth liquid culture medium containing no carbon source and having a concentration of 800mg/L, the number of viable bacteria in the culture medium being 108-1010cfu/mL, results are shown in FIG. 4;
test group 2: inoculating the potential bacteria for degrading ethylene oxide into a liquid culture medium which does not contain a carbon source and contains an ethylene oxide inducer with the concentration of 800mg/L in such a way that 5mL of the bacteria with the living body number of 10 are taken10-1012cfu/mL of the bacterial culture medium to 400mL of a nutrient broth liquid culture medium containing no carbon source and having a concentration of 800mg/L, the number of viable bacteria in the culture medium being 108-1010cfu/mL; the results are shown in FIG. 5;
control group: a nutrient broth liquid medium without carbon source and with an ethylene oxide concentration of 800mg/L, not inoculated with the EO-05 strain/ethylene oxide-degrading potentilla.
Placing the test group and the control group together in a constant temperature box at 37 ℃ for standing culture for 48h, and detecting the degradation result by gas chromatography.
Nutrient broth culture medium: 10g of peptone, 5g of sodium chloride and 1000mL of distilled water are subpackaged into 400mL of standard, sterilized at 121 ℃ for 20min, cooled to room temperature for storage, and 320mg of ethylene oxide is added by using a closed syringe before use to prepare a liquid culture medium which does not contain a carbon source and contains 800mg/L of ethylene oxide.
The detection sample and the control sample are sent to the disease prevention control center of Shaanxi province for gas chromatography detection, and the detection method is according to GB 15979-.
A detection step:
1. dissolving a certain volume of pure ethylene oxide gas in deionized water by using a sealed injector, and preparing an ethylene oxide standard substance with the concentration of 0-200mg/L by gradient dilution;
2. diluting the samples of the test group and the control group by 5 times by using deionized water to prepare samples to be detected;
3. after the instrument is stabilized, under the same condition, 2 mu L of ethylene oxide standard sample and diluted sample to be analyzed are respectively injected, and each sample solution is parallelly tested for 2 times;
4. performing qualitative calculation according to retention time, performing quantitative calculation according to peak area, and taking an average value;
5. according to the measurement data of the ethylene oxide standard substance, making an ethylene oxide standard curve, and calculating the concentration of the ethylene oxide of the test group and the concentration of the ethylene oxide of the control group on the standard curve by using the peak area corresponding to the ethylene oxide in the sample;
6. the ethylene oxide degradation rate was calculated according to the following formula:
degradation rate (control concentration-test concentration)/control concentration
The results are shown in Table 2;
TABLE 2
The result of the scheme is as follows:
the EO-05 can degrade high-concentration ethylene oxide under the condition of no carbon source, and the degradation rate of the ethylene oxide can reach 68.65 percent.
Example 5 identification of ethylene oxide degrading Effect of EO-05 Strain
And (3) microbial culture activation: EO-05 strain was taken out from a freezer at-80 ℃ and 10. mu.L of the strain was inoculated into 100mL of tryptone soybean broth culture medium and cultured at 37 ℃ and 200rpm for 48 hours. The number of thalli in the culture bacterial liquid is 1010-1012cfu/mL。
Test groups:
test group 1: the pure culture strain EO-05 was inoculated into a liquid medium containing no carbon source but containing an ethylene oxide inducer at a concentration of 400mg/L in such a manner that 5mL of the cells were taken as 10 viable cells10-1012cfu/mL EO-05 bacteria culture to 400mL of a nutrient broth liquid culture medium containing no carbon source and having a concentration of 400mg/L, the number of viable bacteria in the culture medium being 108-1010cfu/mL。
Test group 2: inoculating the potential bacteria for degrading ethylene oxide into liquid culture containing no carbon source but containing 400mg/L concentration ethylene oxide inducerIn the culture medium, the inoculation method is to take 5mL of the thallus with the number of living bodies being 1010-1012cfu/mL of the bacterial culture medium to 400mL of a nutrient broth liquid culture medium containing no carbon source and having a concentration of 400mg/L, the number of viable bacteria in the culture medium being 108-1010cfu/mL；
Control group: a nutrient broth liquid medium without carbon source and with an ethylene oxide concentration of 800mg/L, not inoculated with the EO-05 strain/ethylene oxide-degrading potentilla.
Placing the test group and the control group together in a constant temperature box at 37 ℃ for standing culture for 48h, and detecting the degradation result by gas chromatography.
Nutrient broth culture medium: 10g of peptone, 5g of sodium chloride and 1000mL of distilled water are subpackaged into 400mL of standard, sterilized at 121 ℃ for 20min, cooled to room temperature for storage, and 160mg of ethylene oxide is added by using a closed syringe before use to prepare a liquid culture medium which does not contain a carbon source and contains 400mg/L of ethylene oxide.
The detection sample and the control sample are sent to the disease prevention control center of Shaanxi province for gas chromatography detection, and the detection method is according to GB 15979-.
A detection step:
1. dissolving a certain volume of pure ethylene oxide gas in deionized water by using a sealed injector, and preparing an ethylene oxide standard substance with the concentration of 0-200mg/L by gradient dilution;
2. diluting the samples of the test group and the control group by 5 times by using deionized water to prepare samples to be detected;
3. after the instrument is stabilized, under the same condition, 2 mu L of ethylene oxide standard sample and diluted sample to be analyzed are respectively injected, and each sample solution is parallelly tested for 2 times;
4. performing qualitative calculation according to retention time, performing quantitative calculation according to peak area, and taking an average value;
5. according to the measurement data of the ethylene oxide standard substance, making an ethylene oxide standard curve, and calculating the concentration of the ethylene oxide of the test group and the concentration of the ethylene oxide of the control group on the standard curve by using the peak area corresponding to the ethylene oxide in the sample;
6. the ethylene oxide degradation rate was calculated according to the following formula:
degradation rate (control concentration-test concentration)/control concentration;
the results are shown in Table 3;
TABLE 3
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Sequence listing
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Qiaokang Biotechnology (Guangdong) Ltd
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ccgtagctgg tttgagagga cgaccagcca cactgggact gagacacggc ccagactcct 300
acgggaggca gcagtgggga attttggaca atgggggaaa ccctgatcca gccatcccgc 360
gtgtatgatg aaggccttcg ggttgtaaag tacttttggc agagaagaaa aggcatcccc 420
taatacggga tgctgctgac ggtatctgca gaataagcac cggctaacta cgtgccagca 480
gccgcggtaa tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgtgta 540
ggcggttcgg aaagaaagat gtgaaatccc agggctcaac cttggaactg catttttaac 600
tgccgagcta gagtatgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag 660
atatgtggag gaataccgat ggcgaaggca gccccctggg ataatactga cgctcagaca 720
cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccct aaacgatgtc 780
aactagctgt tggggccgtt aggccttagt agcgcagcta acgcgtgaag ttgaccgcct 840
ggggagtacg gtcgcaagat taaaactcaa aggaattgac ggggacccgc acaagcggtg 900
gatgatgtgg attaattcga tgcaacgcga aaaaccttac ctacccttga catgtctgga 960
aagccgaaga gatttggcag tgctcgcaag agaaccggaa cacaggtgct gcatggctgt 1020
cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttgtcatt 1080
agttgctacg caagagcact ctaatgagac tgccggtgac aaaccggagg aaggtgggga 1140
tgacgtcaag tcctcatggc ccttatgggt agggcttcac acgtcataca atggtcggga 1200
cagagggtcg ccaacccgcg agggggagcc aatctcagaa acccgatcgt agtccggatc 1260
gcagtctgca actcgactgc gtgaagtcgg aatcgctagt aatcgcggat cagaatgtcg 1320
cggtgaatac gttcccgggt cttgtacaca ccgcccgtca caccatggga gtgggtttca 1380
ccagaagtag gtagcctaac cgcaaggagg gcgctaccac ggtgatgatg tc 1432
Claims (2)
1. An alcaligenes faecalis characterized in that the strain is preserved in China general microbiological culture Collection center (CGMCC) on 29.8.2019 with the preservation number of CGMCC No.18435 and the preservation address of China academy of sciences microbial research institute No.3 of Beijing West Lu No.1 Hospital in the sunward region of Beijing; the taxonomy of the strain is named Alcaligenes faecalis (Alcaligenes faecalis) and is named EO-05.
2. The use of an alcaligenes faecalis according to claim 1 for the degradation of ethylene oxide.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010064718.2A CN111117932A (en) | 2020-01-20 | 2020-01-20 | Alcaligenes faecalis and application thereof in degradation of ethylene oxide |
| PCT/CN2020/101139 WO2021147260A1 (en) | 2020-01-20 | 2020-07-09 | Alcaligenes faecalis for degrading ethylene oxide and uses thereof |
| US17/012,843 US11124438B2 (en) | 2020-01-20 | 2020-09-04 | Alcaligenes faecalis for degrading ethylene oxide |
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| CN202010064718.2A CN111117932A (en) | 2020-01-20 | 2020-01-20 | Alcaligenes faecalis and application thereof in degradation of ethylene oxide |
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| CN111117932A true CN111117932A (en) | 2020-05-08 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021147260A1 (en) * | 2020-01-20 | 2021-07-29 | Qiaokang Biotech (Guangdong) Co., LTD. | Alcaligenes faecalis for degrading ethylene oxide and uses thereof |
| US11085016B1 (en) | 2020-01-20 | 2021-08-10 | Chio Kang Medical, Inc. | Method for screening bacteria capable of degrading ethylene oxide |
| US11124438B2 (en) | 2020-01-20 | 2021-09-21 | Chio Kang Medical, Inc. | Alcaligenes faecalis for degrading ethylene oxide |
| US11130939B2 (en) | 2020-01-20 | 2021-09-28 | Chio Kang Medical, Inc. | Bacteria for degrading ethylene oxide and uses thereof |
| US11220667B2 (en) | 2020-01-20 | 2022-01-11 | Chio Kang Medical, Inc. | Bacteria for degrading ethylene oxide and applications thereof |
-
2020
- 2020-01-20 CN CN202010064718.2A patent/CN111117932A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021147260A1 (en) * | 2020-01-20 | 2021-07-29 | Qiaokang Biotech (Guangdong) Co., LTD. | Alcaligenes faecalis for degrading ethylene oxide and uses thereof |
| US11085016B1 (en) | 2020-01-20 | 2021-08-10 | Chio Kang Medical, Inc. | Method for screening bacteria capable of degrading ethylene oxide |
| US11124438B2 (en) | 2020-01-20 | 2021-09-21 | Chio Kang Medical, Inc. | Alcaligenes faecalis for degrading ethylene oxide |
| US11130939B2 (en) | 2020-01-20 | 2021-09-28 | Chio Kang Medical, Inc. | Bacteria for degrading ethylene oxide and uses thereof |
| US11220667B2 (en) | 2020-01-20 | 2022-01-11 | Chio Kang Medical, Inc. | Bacteria for degrading ethylene oxide and applications thereof |
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