CN111111608A - Method for selectively removing traditional Chinese medicine notoginseng pigment by adopting silicate minerals - Google Patents
Method for selectively removing traditional Chinese medicine notoginseng pigment by adopting silicate minerals Download PDFInfo
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- CN111111608A CN111111608A CN201911381617.1A CN201911381617A CN111111608A CN 111111608 A CN111111608 A CN 111111608A CN 201911381617 A CN201911381617 A CN 201911381617A CN 111111608 A CN111111608 A CN 111111608A
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- ginseng
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
- B01J20/10—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
- B01J20/12—Naturally occurring clays or bleaching earth
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
Abstract
The invention discloses a method for selectively removing traditional Chinese medicine notoginseng pigment by using silicate minerals, which comprises the steps of doping clay in a certain proportion as an adsorbent by using silicate mineral attapulgite in a certain proportion as a matrix, filling the mixture into an SPE small column, activating the adsorbent by using 0.1M HCl solution in a certain volume, preparing a notoginseng solution in a certain concentration, passing through the SPE small column, and enriching the pigment in notoginseng in the SPE small column by using a solid phase extraction technology. Can effectively remove the pigment in the traditional Chinese medicine pseudo-ginseng, the decolorization rate reaches 28 to 34 percent, and the loss rate of the active ingredient pseudo-ginseng total saponin is only 0 to 5 percent, thereby realizing that the pigment substance in the pseudo-ginseng can be selectively removed and the effective active ingredient pseudo-ginseng total saponin is reserved.
Description
Technical Field
The invention relates to the technical field of separation and purification, in particular to a method for selectively removing traditional Chinese medicine pseudo-ginseng pigment by adopting silicate minerals.
Background
Pseudo-ginseng is a Chinese medicine, mainly produced in Yunnan Wenshan, so the Wenshan pseudo-ginseng, also the Wenshan pseudo-ginseng, is the dry root and rhizome of Panax notoginseng of Araliaceae. Digging before blossom in autumn, cleaning, separating main root, branch root and rhizome, and drying. The rootlets are called "ribs" and the rootstocks are called "cut". The medicine parts are dry roots and rhizomes of plants, and are sweet, slightly bitter and warm in nature; the main effects of the hemostatic plaster are good hemostatic effect and obvious hematopoietic function; can strengthen and improve coronary microcirculation, and dilate blood vessel; relieving pain, and has effects of relieving fatigue, and improving learning and memory ability; anti-inflammatory effects; anti-tumor effect; anti-aging and anti-oxidation effects; reducing blood lipid and cholesterol. The main active component of the panax notoginseng is panax notoginseng saponins, and the content of the panax notoginseng saponins is 8 to 12 percent. In addition, the pseudo-ginseng also contains compounds such as alcohols, flavonoids, nucleosides, alkaloids, proteins, vitamin C and the like and more than 18 inorganic elements such as potassium, magnesium, calcium and the like, thereby being the special basis of the pseudo-ginseng with various effects. (Zhang Xiping, Qili, Liu Da ren, Sanchi and their effective components pharmacological action research status, review and progress, 2007,36:96-98.)
Generally, the panax notoginseng saponins are extracted by a cold leaching method, water is used as an extraction solvent, the extraction time is 24 hours, and the total panax notoginseng saponins extracted under the condition have the strongest platelet aggregation resistance; the acid hydrolysis method, hydrolyzing the pseudo-ginseng crude powder for 6 hours at 60 ℃ by using acetic acid and ethanol in a ratio of 1:1, can easily extract glycosides, but has low acid hydrolysis selectivity and low hydrolysis nonuniform extraction efficiency, and can easily hydrolyze the same saponin into different products; the percolation method is a better extraction method, has the advantages of simple equipment, safe operation, energy conservation, consumption reduction, reduced component damage and the like, but consumes longer time and is not suitable for large-scale industrial production; the ultrasonic enzyme method has the optimal extraction conditions of pH4.5 and 50 ℃ of enzymolysis temperature, cellulase, pectinase, enzymolysis time of 2h, ultrasonic time of 90min and water addition amount of 8 times, and the ultrasonic extraction method and the biological enzyme extraction method are novel methods for extracting effective components of modern Chinese medicaments, are simple and convenient to operate, have stable process conditions, and have the extract yield remarkably higher than that of the traditional method; the supercritical extraction method has the advantages that the extraction temperature suitable for the crushing process is 45 ℃, the pressure is 38MPa, the flow is 23kg/h, the entrainer is 300ml, the extraction time is 3h, the supercritical extraction technology has the advantages of strong extraction capacity, high extraction rate, short production period, undamaged effective components, simple process, easily controlled operation parameters, no solvent residue, stable product quality and the like, and is applied to the industrial mass production of the effective components of the traditional Chinese medicine; the water extraction method uses water as a solvent, applies modern scientific technologies such as biological enzyme, fermentation, combination of macroporous adsorption resin, decolorizing resin, ion exchange resin and the like to extract, separate, refine and purify the panax notoginseng saponins, and has the advantages of high efficiency, environmental protection, excellent and stable product quality and industrial large-scale production.
The attapulgite clay is a clay mineral with attapulgite as main component. Attapulgite is a crystalline hydrated magnesium aluminum silicate mineral, has unique layer chain structure characteristic, and has lattice displacement in its structure, and the crystal contains variable amount of Na+、Ca2+、Fe3+、Al3+The crystals are needle-shaped, fibrous or fibrous aggregates. The attapulgite has good colloidal properties of unique dispersion, high temperature resistance, salt and alkali resistance and the like and higher adsorption decoloring capacity. The adhesive has certain plasticity and binding power, and the ideal chemical molecular formula is as follows: mg (magnesium)5Si8O20(OH)2(OH)4·4H2And O. Having an intermediate structure between the chain-like structure and the layered structure. The attapulgite is produced in sedimentary rock and weathering crust in the form of soil and compact blocks, and has white, off-white, grey green or weak sericin luster. The soil is fine and smooth, has a greasy feeling, is light and crisp, has shell-shaped or ragged fracture and strong water absorption. Has viscosity and plasticity when wet, shrinks less after drying, does not show cracks greatly, and is soaked in water to be disintegrated. The suspension does not flocculate and precipitate when encountering dielectrics. The activated clay is an adsorbent made up by using bentonite as raw material, and making it pass through such processes of inorganic acidification, salt treatment or other treatment, water rinsing and drying, and its appearance is milk white powder, odorless, tasteless, non-toxic and adsorption propertyIt is strong and can adsorb colored substances and organic substances. It is easy to absorb moisture in the air, and the adsorption performance is reduced after the storage for a long time. When in use, the materials are heated (preferably to 80-100 ℃) for reactivation, but when the materials are heated to more than 300 ℃, crystal water begins to lose, the structure is changed, and the fading effect is influenced. Activated clay is insoluble in water, organic solvents and various oils, and is almost completely soluble in hot caustic soda and hydrochloric acid. Halloysite Nanotubes (HNTs) are a class of aluminosilicate materials, named by Berthier in 1826. In 2006, Natual Nano reported that it was dispersed in a polypropylene composite material, which had significantly improved flexural strength and impact strength compared to that before its addition. From this time, halloysite nanotubes have attracted a great deal of attention in the scientific community. Researches find that the halloysite nanotube has the characteristics of large specific surface area, rich reserves, good thermal stability, uniform structure and the like, so that the halloysite nanotube is widely applied to the fields of catalysis, drug sustained release, composite materials, adsorption and the like. (Marizhi, Dongjunjie, Liying Qian, Zhuwei, Liuhuan, He kang, structural modification in halloysite nanotubes and performance research thereof, and silicate report, 2017,36(8):2851-2860.) while the halloysite nanotubes have a similar pipeline structure to carbon nanotubes, which is called a substitute material of carbon nanotubes. In the field of traditional Chinese medicine decolorization, compared with an activated carbon material, silicate minerals such as attapulgite, activated clay and the like have little adsorption on active ingredients of traditional Chinese medicines and have selectivity on adsorption of pigments.
Disclosure of Invention
The invention aims to retain the main active ingredient panax notoginseng saponins in panax notoginseng in order to selectively remove the panax notoginseng pigments, and provides a method for selectively adsorbing the pigments in traditional Chinese medicine panax notoginseng without retaining the main saponin ingredients in panax notoginseng by taking silicate mineral attapulgite as a matrix and doping other clays as an adsorbent. In order to achieve the purpose, the technical scheme of the invention is realized as follows:
loading 50-90% silicate mineral attapulgite as matrix doped with 10-50% clay as adsorbent into SPE column, activating the adsorbent with 6-13ml 0.1M HCl solution to obtain 6-13mg/ml Notoginseng radix solution, passing through SPE column, and enriching pigment in Notoginseng radix with solid phase extraction technology.
Preferably, the specific surface area of the attapulgite is 110-150m2In the range of/g.
Preferably, the attapulgite has a pore volume of 0.10-0.25cm3In terms of a/g, the mean pore diameter is in the range from 3.5 to 6.5 nm.
Preferably, the clay is one or more of activated clay, halloysite and kaolin.
The invention has the beneficial effects that: a method for selectively removing pigment from Notoginseng radix by using silicate mineral comprises using attapulgite as matrix and mixing with clay as adsorbent to effectively remove pigment from Notoginseng radix with decolorization rate of 28-34% and loss rate of active component Notoginseng radix total saponin of only 0-5%, so as to selectively remove pigment from Notoginseng radix and retain effective active component Notoginseng radix total saponin.
Drawings
Fig. 1 is a color change of an aqueous solution of notoginseng before and after decolorization, wherein the color changes of examples 1 to 6 are before and after decolorization in order from left to right.
FIG. 2 is a graph showing the color change of Notoginseng radix powder before and after decolorization;
FIG. 3 shows the HPLC analysis results of the Notoginseng radix solution before and after decolorization, wherein the HPLC analysis results of Notoginseng radix stock solution and examples 1-6 are shown from top to bottom.
Detailed Description
The core of the specific implementation mode of the invention is to provide a method for selectively removing traditional Chinese medicine notoginseng pigment by using silicate minerals, which is characterized in that 50-90% of silicate mineral attapulgite is used as a matrix, 10-50% of clay is doped as an adsorbent, the adsorbent is filled into an SPE small column, 6-13ml of 0.1M HCl solution is used for activating the adsorbent, 6-13mg/ml notoginseng solution is prepared, and then the mixture passes through the SPE small column, and the solid phase extraction technology is utilized to enrich the pigment in notoginseng in the SPE small column.
Preferably, the specific surface area of the attapulgite is 110-150m2In the range of/g.
Preferably, the attapulgite has a pore volume of 0.10-0.25cm3In g, the average pore diameter is in the range of 3.5-6.5nm。
Preferably, the clay is one or more of activated clay, halloysite and kaolin.
So that the manner in which the features and advantages of the present invention can be understood in detail, a more particular description of the invention, briefly summarized above, may be had by reference to embodiments, some of which are illustrated in the appended drawings.
Example 1
Mixing 1g of attapulgite and 0.6g of activated clay, loading into an SPE cartridge, activating an adsorbent with 0.1M HCl solution, flushing the SPE cartridge to neutrality with deionized water, allowing 10ml of 9mg/ml panax notoginseng solution to pass through the SPE cartridge, collecting a flow-through liquid and an eluent, freezing for 5h in a refrigerator at-35 ℃, transferring to a freeze dryer, and freeze-drying to obtain decolorized panax notoginseng powder.
Example 2
Mixing 1.5g of attapulgite and 0.4g of activated clay, loading into an SPE cartridge, activating an adsorbent with 0.1M HCl solution, flushing the SPE cartridge to neutrality with deionized water, allowing 10ml of 11mg/ml of radix Notoginseng solution to pass through the SPE cartridge, collecting a flow-through solution and a leacheate, freezing in a refrigerator at-35 ℃ for 5h, transferring to a freeze dryer, and freeze-drying to obtain decolorized radix Notoginseng powder.
Example 3
Mixing 1g of attapulgite and 0.5g of halloysite, loading into an SPE small column, activating an adsorbent with 0.1M HCl solution, flushing the SPE small column to be neutral with deionized water, allowing 10ml of 10mg/ml pseudo-ginseng solution to pass through the SPE small column, collecting a flow-through liquid and an eluent, freezing for 5 hours in a refrigerator at-35 ℃, transferring to a freeze dryer, and freeze-drying to obtain decolorized pseudo-ginseng powder.
Example 4
Mixing 1.2g of attapulgite and 0.5g of kaolin, loading into an SPE cartridge, activating an adsorbent with 0.1M HCl solution, flushing the SPE cartridge to neutrality with deionized water, passing 10ml of 11mg/ml of pseudo-ginseng solution through the SPE cartridge, collecting a flow-through liquid and an eluent, freezing for 5 hours in a refrigerator at-35 ℃, transferring to a freeze dryer, and freeze-drying to obtain decolorized pseudo-ginseng powder.
Example 5
Mixing 1g of attapulgite and 0.3g of halloysite, loading into an SPE small column, activating an adsorbent with 0.1M HCl solution, flushing the SPE small column to be neutral with deionized water, allowing 10ml of 9mg/ml pseudo-ginseng solution to pass through the SPE small column, collecting a flow-through liquid and an eluent, freezing for 5 hours in a refrigerator at-35 ℃, transferring to a freeze dryer, and freeze-drying to obtain decolorized pseudo-ginseng powder.
Example 6
Mixing 1.2g of attapulgite and 0.6g of kaolin, loading into an SPE cartridge, activating an adsorbent with 0.1M HCl solution, flushing the SPE cartridge to neutrality with deionized water, passing 10ml of 10mg/ml of pseudo-ginseng solution through the SPE cartridge, collecting a flow-through liquid and an eluent, freezing for 5 hours in a refrigerator at-35 ℃, transferring to a freeze dryer, and freeze-drying to obtain decolorized pseudo-ginseng powder.
As shown in FIG. 1, the color of the stock solution of Panax notoginseng before decolorization and the solutions of Panax notoginseng of examples 1-6 after decolorization are changed from left to right. As shown in FIG. 2, it can be seen that the decoloring effect is visible to the naked eye after the method of the present invention is applied. Quantitatively analyzing Notoginseng radix solution before and after decolorization by high performance liquid chromatography, adopting Tnature C18 chromatographic column, Waters 2695(2998PDADetector) as HPLC apparatus, detecting wavelength of 203nm, flow rate of 1.0mL/min, column temperature of 25 deg.C, sample injection volume of 10 μ L, and mobile phase of acetonitrile and water (20:80-55: 45). The test results are shown in table 1 and figure 3, the method effectively removes the pigment in the traditional Chinese medicine panax notoginseng, the decolorization rate reaches 28-33.3%, the loss rate of the panax notoginseng saponins as the active ingredient is only 0-4.59%, and the selective removal of the pigment substances in the panax notoginseng and the retention of the panax notoginseng saponins as the active ingredient are realized.
Decolorization rate before and after surface decolorization and saponin recovery rate
The above-mentioned general knowledge is not described in detail, and can be understood by those skilled in the art.
The present invention is not intended to be limited to the particular embodiments shown and described, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (4)
1. A method for selectively removing traditional Chinese medicine pseudo-ginseng pigment by adopting silicate minerals is characterized by comprising the following steps: mixing 50-90% silicate mineral attapulgite as matrix with 10-50% clay as adsorbent, loading into SPE column, activating the adsorbent with 6-13ml 0.1M HCl solution to obtain 6-13mg/ml Notoginseng radix solution, passing through SPE column, and enriching pigment in Notoginseng radix with solid phase extraction technology.
2. The method for selectively removing the pigment of the pseudo-ginseng with silicate minerals according to claim 1, which is characterized in that: the specific surface area of the attapulgite is 110-150m2In the range of/g.
3. The method for selectively removing the pigment of the traditional Chinese medicine pseudo-ginseng by adopting the silicate minerals as claimed in claim 1, which is characterized in that: the pore volume of the attapulgite is 0.10-0.25cm3In terms of a/g, the mean pore diameter is in the range from 3.5 to 6.5 nm.
4. The method for selectively removing the pigment of the traditional Chinese medicine pseudo-ginseng by adopting the silicate minerals as claimed in claim 1, which is characterized in that: the clay is one or more of activated clay, halloysite and kaolin.
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| CN201911381617.1A CN111111608A (en) | 2019-12-27 | 2019-12-27 | Method for selectively removing traditional Chinese medicine notoginseng pigment by adopting silicate minerals |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5311886A (en) * | 1991-12-31 | 1994-05-17 | Imasco Limited | Tobacco extract treatment with insoluble adsorbent |
| CN104096543A (en) * | 2014-06-23 | 2014-10-15 | 中国科学院广州能源研究所 | Preparation method for organic activated clay used for decolorization |
| CN104525101A (en) * | 2014-11-24 | 2015-04-22 | 江苏澳特邦非金属矿业有限公司 | Preparation method of attapulgite-based adsorption decolorizing agent used for soybean oil refining |
| CN106198172A (en) * | 2015-04-30 | 2016-12-07 | 中国科学院大连化学物理研究所 | A kind of selective enrichment method of tree peony anthocyanins in black Fructus Lycii |
| CN106925211A (en) * | 2017-04-14 | 2017-07-07 | 明光市国星凹土有限公司 | A kind of fat decolourizing agent with modified attapulgite as carrier |
| CN108160027A (en) * | 2018-01-07 | 2018-06-15 | 南京审计大学 | A kind of preparation method of high-efficiency grease decolorising agent composition |
| CN110407890A (en) * | 2019-07-18 | 2019-11-05 | 佛山市欧若拉生物科技有限公司 | The preparation method of sapindoside |
-
2019
- 2019-12-27 CN CN201911381617.1A patent/CN111111608A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5311886A (en) * | 1991-12-31 | 1994-05-17 | Imasco Limited | Tobacco extract treatment with insoluble adsorbent |
| CN104096543A (en) * | 2014-06-23 | 2014-10-15 | 中国科学院广州能源研究所 | Preparation method for organic activated clay used for decolorization |
| CN104525101A (en) * | 2014-11-24 | 2015-04-22 | 江苏澳特邦非金属矿业有限公司 | Preparation method of attapulgite-based adsorption decolorizing agent used for soybean oil refining |
| CN106198172A (en) * | 2015-04-30 | 2016-12-07 | 中国科学院大连化学物理研究所 | A kind of selective enrichment method of tree peony anthocyanins in black Fructus Lycii |
| CN106925211A (en) * | 2017-04-14 | 2017-07-07 | 明光市国星凹土有限公司 | A kind of fat decolourizing agent with modified attapulgite as carrier |
| CN108160027A (en) * | 2018-01-07 | 2018-06-15 | 南京审计大学 | A kind of preparation method of high-efficiency grease decolorising agent composition |
| CN110407890A (en) * | 2019-07-18 | 2019-11-05 | 佛山市欧若拉生物科技有限公司 | The preparation method of sapindoside |
Non-Patent Citations (8)
| Title |
|---|
| TENG CHEN ET AL.: "Process development for the decoloration of Panax notoginseng extracts: A design space approach", 《JOURNAL OF SEPARATION SCIENCE》 * |
| 余芳等: "埃洛石吸附-介质阻挡放电等离子体处理亚甲基蓝废水", 《工业水处理》 * |
| 刘俊建等: "《水处理过程中典型难降解有机物迁移转化的数值模拟》", 30 November 2015, 石家庄：河北人民出版社 * |
| 孙成贺等: "西洋参果脱色方法比较", 《江苏农业科学》 * |
| 李东等: "三七总皂苷纯化和脱色工艺研究", 《海峡药学》 * |
| 聂继云等: "《果品及其制品质量安全检测营养品质和功能成分》", 31 December 2017, 北京：中国质检出版社 * |
| 马丽娜等: "Freundlich方程在油脂脱色体系中的应用及其返色研究", 《食品科学》 * |
| 马智等: "埃洛石纳米管管内结构改造及其性能的研究", 《硅酸盐通报》 * |
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