CN111097561A - Controllable negative pressure continuous liquid suction gun for cell culture - Google Patents
Controllable negative pressure continuous liquid suction gun for cell culture Download PDFInfo
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- CN111097561A CN111097561A CN202010070614.2A CN202010070614A CN111097561A CN 111097561 A CN111097561 A CN 111097561A CN 202010070614 A CN202010070614 A CN 202010070614A CN 111097561 A CN111097561 A CN 111097561A
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- pipette
- suction gun
- negative pressure
- silica gel
- liquid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0213—Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a controllable negative pressure continuous liquid suction gun for cell culture, which comprises a liquid suction gun body, a liquid suction gun barrel, a liquid suction gun head and a silica gel guide pipe, wherein a hollow accommodating cavity is formed in the liquid suction gun body, the silica gel guide pipe penetrates through the accommodating cavity, extends into the liquid suction gun barrel and is butted with a needle-shaped metal pipe arranged in the liquid suction gun head, the other end of the silica gel guide pipe extends out of the liquid suction gun body and is connected with a negative pressure suction filtration device, a pressure rod is further arranged in the accommodating cavity, the middle position of the pressure rod is connected with the inside of the accommodating cavity through a pin shaft, the pressure rod can rotate around the pin shaft, and a control mechanism for controlling the rotation. The silica gel guide pipe is arranged, so that the liquid guide and convenient liquid flow control effects are achieved; the horn-shaped liquid suction gun head is convenient to suck liquid without residual liquid; the inner diameter of the needle-shaped metal tube is not more than 1mm, so that the largest possible liquid surface tension is ensured, and the backflow of a small amount of residual liquid is prevented.
Description
Technical Field
The invention belongs to the technical field of experimental instruments, and particularly relates to a controllable negative pressure continuous liquid suction gun for cell culture.
Background
The liquid is a conventional operation to the absorption of cell sterile culture in-process, the prior art is operated with straw or pipette, also there is the straw mode of connecing the negative pressure like the chinese utility model patent of publication No. CN206168453U, but all there is not enough, the former imbibition volume is limited, generally about 10ml, straw or pipette are all that manual operation carries out the absorption of liquid and abandons the liquid, manual operation is more loaded down with trivial details hard, and frequent replacement, operating time is long easily pollutes, the latter is more low-level extensive, continuity practicality technical difference.
Disclosure of Invention
In view of the problems in the prior art, the invention aims to provide a cell culture controllable negative pressure continuous liquid suction gun which has the advantages of large liquid suction amount, strong continuity, simple operation, quickness and high efficiency, can effectively prevent pollution and can quickly suck and discard liquid in a common laboratory.
A controllable negative pressure of cell culture inhales liquid rifle in succession, including the imbibition rifle body, imbibition barrel and with imbibition rifle head, silica gel pipe that the imbibition barrel links to each other, its characterized in that: the utility model discloses a suction gun, including the imbibition rifle body, the internal portion of imbibition rifle is the hollow chamber that holds, silica gel pipe runs through and holds the chamber after extend to in the imbibition rifle pipe to peg graft with the needle-like tubular metal resonator that sets up on the imbibition rifle head and link to each other, the silica gel pipe other end extends the imbibition rifle external and links to each other with negative pressure suction filtration device, it still is provided with the control mechanism who is used for controlling the imbibition state to hold the.
Control mechanism includes depression bar, control lever, first spring, the middle part position of depression bar through the round pin axle with hold intracavity wall connection, and the depression bar can rotate around the round pin axle, the top of the imbibition rifle body is stretched out to the one end of control lever, and the other end extends to and holds the intracavity to touch with the one end of depression bar and connect, the depression bar is kept away from the one end of control lever and is pressed on the silica gel pipe, first spring cup joints the setting on the control lever, and is provided with the arch that is used for fixed first spring both ends on the control lever.
One side of the pressure lever close to the silica gel conduit is also provided with a second spring, and the other end of the second spring is fixedly connected with the inner wall of the accommodating cavity.
The needle-shaped metal tube and the liquid suction gun head are integrally arranged, and the needle-shaped metal tube extends out of the liquid suction gun head and then is connected with the silica gel guide tube in an inserted mode.
The liquid suction gun body consists of a shell and a detachable side cover arranged on the shell.
The lower end of the liquid suction gun head is in arc-shaped shrinkage from the edge part to the center position to form a thin tube, the diameter of the thin tube is the same as the aperture of the needle-shaped metal tube, and the thin tube is in butt joint with the tube end of the needle-shaped metal tube and is fixed into a whole.
The rotation angle of the pressure lever around the pin shaft is 30-60 degrees.
The imbibition rifle head and imbibition barrel threaded connection make things convenient for ageing, the consume of later stage silicone tube and this imbibition rifle head to change.
The lower end of the liquid suction gun head is sleeved with a common tip head, and 200ul is preferably selected as the optimal point, and an outward push rod for removing the tip head is arranged on the liquid suction gun body.
The inner diameter of the needle-shaped metal tube in the liquid suction gun head is not more than 1 mm.
The silica gel guide pipe is arranged, so that the liquid guide and convenient liquid flow control effects are achieved; the horn-shaped liquid suction gun head is convenient to suck liquid without residual liquid; the inner diameter of the needle-shaped metal tube is not more than 1mm, so that the largest possible liquid surface tension is ensured, and the backflow of a small amount of residual liquid is prevented.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the internal structure of the gun body according to the present invention;
FIG. 3 is a schematic structural view of a pipette tip of the present invention;
in the figure: 1-liquid suction gun body, 2-liquid suction gun barrel, 3-liquid suction gun head, 4-silica gel guide pipe, 5-needle-shaped metal pipe, 6-pressure rod, 7-pin shaft, 8-control rod, 9-first spring, 10-second spring, 11-tip head and 12-external push rod.
Detailed Description
The invention will be further described with reference to the accompanying drawings, to which, however, the scope of the invention is not limited.
As shown in figures 1-3, the invention relates to a controllable negative pressure continuous liquid suction gun body for cell culture, which comprises a liquid suction gun body, a liquid suction gun barrel, a liquid suction gun head inserted into the liquid suction gun barrel, and a silica gel guide tube, wherein the liquid suction gun body comprises a shell and a detachable side cover arranged on the shell, a hollow accommodating cavity is arranged in the liquid suction gun body, the silica gel guide tube penetrates through the accommodating cavity and extends into the liquid suction gun barrel and is butted with a needle-shaped metal tube arranged in the liquid suction gun head, the lower end of the silica gel guide tube is inserted into the needle-shaped metal tube, the needle-shaped metal tube is integrally arranged on the liquid suction gun head, the other end of the silica gel guide tube extends out of the liquid suction gun body and is connected with a negative pressure suction filtration device, such as a medical suction bottle, a waste liquid bottle and the like, the silica gel guide tube plays a role in guiding liquid and facilitating liquid flow control, the lower end, and the lower end of the liquid suction gun head is sleeved with a common tip head, an outer push rod for removing the tip head is arranged on the liquid suction gun body, the connecting structure of the outer push rod and the tip head is the conventional technology, and the drawing and the text part are not specifically shown.
Further, still be provided with on the imbibition rifle body and be used for controlling depression bar pivoted control mechanism, control mechanism includes the depression bar, the control lever, first spring, the second spring, the middle part position of depression bar holds intracavity portion through the round pin axle and links, and the depression bar can rotate 30-60 degrees around the round pin axle, the top of the imbibition rifle body is stretched out to the one end of control lever, the other end extends to and holds the intracavity, and touch with the one end of depression bar and connect, the depression bar other end is pressed on the silica gel pipe, the spring cup joints the setting on the control lever, and be provided with the arch that is used for the fixed spring both ends on its control lever. The second spring is close to one side of silica gel pipe and sets up, and the one end and the depression bar fixed connection of second spring, the other end and the holding chamber inner wall fixed connection of second spring. Under the combined action of the control rod and the first spring, one end of the pressing rod far away from the control rod is ensured to press the silica gel conduit, and the second spring acts on the pressing rod to be convenient for resetting.
When the liquid suction device is used, when the control rod is pressed by a thumb, the control rod moves downwards, the first spring is compressed, one end of the pressure rod is extruded, the pressure rod rotates around the fulcrum by 30-60 degrees, meanwhile, the other end of the pressure rod moves oppositely to reduce the extrusion on the silica gel catheter, the silica gel catheter is unobstructed, and liquid suction is completed. By controlling the pressing force, the speed of imbibition can be controlled, continuous imbibition is ensured, 75% alcohol is inhaled by using the imbibition gun head before imbibition for disinfection, and part of the needle-shaped metal tube and the silica gel guide tube are disinfected by the disinfected alcohol. The outer end of the needle-shaped metal tube in the liquid suction gun head is sunken into a horn shape, so that liquid can be conveniently absorbed without residual liquid. The inner diameter of the needle-shaped metal tube in the liquid suction gun head is not more than 1mm so as to ensure the liquid surface tension as large as possible, and when the negative pressure is cut off, the backflow of a small amount of residual liquid is prevented. When absorbing liquid, the liquid absorbing gun head is immersed below the liquid surface of the absorbed liquid, and the switch of the negative pressure suction filtration device connected with the upper end of the silica gel guide pipe is switched on to absorb liquid. The material of the liquid suction gun head can be PVDF and ceramic, the PVDF material is preferably selected in the invention, and has the advantages of firmness, chemical corrosion resistance and heat transfer resistance; 200 mu l and 1000 mu l tip heads can be connected outside the pipette tip head, the caliber of the 200 mu l tip head is optimized, the tip head is mainly common and low in cost, contacts a cell culture system, and is directly discarded after use to prevent pollution. Treat that the imbibition accomplishes the back and loosen the thumb, close the negative pressure device switch, first spring makes the control lever return, has just also loosened the depression bar, makes the depression bar reset under the effect of second spring, oppresses the silica gel pipe, closes the silica gel pipe, accomplishes an imbibition promptly. When the silica gel guide tube needs to be replaced, only the liquid suction gun head is screwed, so that the replacement is convenient and simple.
Claims (10)
1. The utility model provides a controllable negative pressure of cell culture inhales liquid rifle in succession, including the imbibition rifle body (1), imbibition barrel (2) and with imbibition rifle head (3), silica gel pipe (4) that imbibition barrel (2) link to each other, its characterized in that: the utility model discloses a suction gun, including suction gun body (1), silica gel pipe (4) and the cavity of holding, the cavity of holding is inside to be hollow, silica gel pipe (4) run through and hold the cavity back and extend to in suction gun barrel (2) to peg graft with needle-like tubular metal resonator (5) that set up on suction gun head (3) and link to each other, silica gel pipe (4) other end extends suction gun body (1) outer and links to each other with negative pressure suction filtration device, it still is provided with the control mechanism who is used for controlling the.
2. The cell culture controllable negative pressure continuous pipette gun according to claim 1, wherein the control mechanism comprises a pressure lever (6), a control lever (8) and a first spring (9), the middle position of the pressure lever (6) is connected with the inner wall of the containing cavity through a pin shaft (7), the pressure lever (6) can rotate around the pin shaft (7), one end of the control lever (8) extends out of the top of the pipette gun body (1), the other end of the control lever extends into the containing cavity and contacts with one end of the pressure lever (6), one end of the pressure lever (6) far away from the control lever (8) is pressed on the silica gel guide tube (4), the first spring (9) is sleeved on the control lever (8), and the control lever (8) is provided with protrusions for fixing two ends of the first spring (9).
3. The cell culture controllable negative pressure continuous pipette as claimed in claim 2, wherein a second spring (10) is further disposed on one side of the pressure lever (6) close to the silica gel conduit (4), and the other end of the second spring (10) is fixedly connected with the inner wall of the accommodating cavity.
4. The cell culture controllable negative pressure continuous pipette according to claim 1, wherein the needle-shaped metal tube (5) and the pipette head (3) are integrally arranged, and the needle-shaped metal tube (5) extends out of the pipette head (3) and is connected with the silica gel guide tube (4) in an inserting manner.
5. The cell culture controlled negative pressure continuous pipette as claimed in claim 1, wherein the pipette gun body (1) is composed of a housing and a detachable side cover arranged on the housing.
6. The cell culture controllable negative pressure continuous pipette according to claim 1, wherein the lower end of the pipette tip (3) is arc-shaped contracted from the edge to the center to form a thin tube, the diameter of the thin tube is the same as the diameter of the needle-shaped metal tube (5), and the thin tube is butted with and fixed to the end of the needle-shaped metal tube (5).
7. The cell culture controlled negative pressure continuous pipette as claimed in claim 1, wherein the rotation angle of the pressure lever (6) around the pin (7) is 30-60 degrees.
8. The cell culture controlled negative pressure continuous pipette gun according to claim 1, wherein the pipette gun head (3) is connected with the pipette gun barrel (2) by screw threads.
9. The cell culture controlled negative pressure continuous pipette as claimed in claim 1, wherein the lower end of the pipette tip (3) is sleeved with a common tip head (11), and the pipette tip body (1) is provided with an external push rod (12) for removing the tip head (11).
10. A cell culture controlled negative pressure continuous pipette as claimed in claim 1, wherein the inner diameter of the needle-like metal tube (5) in the pipette tip (3) is not more than 1 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010070614.2A CN111097561B (en) | 2020-01-21 | 2020-01-21 | Controllable negative pressure continuous liquid suction gun for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010070614.2A CN111097561B (en) | 2020-01-21 | 2020-01-21 | Controllable negative pressure continuous liquid suction gun for cell culture |
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| Publication Number | Publication Date |
|---|---|
| CN111097561A true CN111097561A (en) | 2020-05-05 |
| CN111097561B CN111097561B (en) | 2022-02-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010070614.2A Active CN111097561B (en) | 2020-01-21 | 2020-01-21 | Controllable negative pressure continuous liquid suction gun for cell culture |
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010286004A (en) * | 2009-06-09 | 2010-12-24 | Nippon Techno:Kk | Remote control of manual valve |
| CN201823546U (en) * | 2010-10-27 | 2011-05-11 | 西北农林科技大学 | Novel automatic suction-discharge type calibrated suction pipe capable of preventing excessive suction |
| CN103196706A (en) * | 2013-03-19 | 2013-07-10 | 重庆大学 | Multi-layer sampler for underflow layer |
| CN203602627U (en) * | 2013-10-15 | 2014-05-21 | 韩山师范学院 | Multipurpose cell culture waste liquid suction-removal device |
| CN204999896U (en) * | 2015-09-30 | 2016-01-27 | 烟台毓璜顶医院 | Collection device of cell culture liquid |
| CN107029309A (en) * | 2017-05-17 | 2017-08-11 | 上海交通大学医学院附属第九人民医院 | A kind of injector for medical purpose and application method for facilitating drawing liquid |
| CN206587778U (en) * | 2016-11-28 | 2017-10-27 | 河南理工大学 | A kind of continuous liquid sucking device of non-equivalent |
| CN206996635U (en) * | 2017-06-15 | 2018-02-13 | 海门中科基因生物科技有限公司 | High flux pipettor |
| CN207221952U (en) * | 2017-07-11 | 2018-04-13 | 长江大学 | A kind of detachable suction head type liquid absorption device of anti-backflow |
| CN207287472U (en) * | 2017-03-17 | 2018-05-01 | 中国农业科学院茶叶研究所 | A kind of continuous liquid-moving machine |
| US20190358624A1 (en) * | 2018-05-23 | 2019-11-28 | Vistalab Technologies, Inc. | Method and apparatus for dispensing precise aliquots of liquid |
-
2020
- 2020-01-21 CN CN202010070614.2A patent/CN111097561B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010286004A (en) * | 2009-06-09 | 2010-12-24 | Nippon Techno:Kk | Remote control of manual valve |
| CN201823546U (en) * | 2010-10-27 | 2011-05-11 | 西北农林科技大学 | Novel automatic suction-discharge type calibrated suction pipe capable of preventing excessive suction |
| CN103196706A (en) * | 2013-03-19 | 2013-07-10 | 重庆大学 | Multi-layer sampler for underflow layer |
| CN203602627U (en) * | 2013-10-15 | 2014-05-21 | 韩山师范学院 | Multipurpose cell culture waste liquid suction-removal device |
| CN204999896U (en) * | 2015-09-30 | 2016-01-27 | 烟台毓璜顶医院 | Collection device of cell culture liquid |
| CN206587778U (en) * | 2016-11-28 | 2017-10-27 | 河南理工大学 | A kind of continuous liquid sucking device of non-equivalent |
| CN207287472U (en) * | 2017-03-17 | 2018-05-01 | 中国农业科学院茶叶研究所 | A kind of continuous liquid-moving machine |
| CN107029309A (en) * | 2017-05-17 | 2017-08-11 | 上海交通大学医学院附属第九人民医院 | A kind of injector for medical purpose and application method for facilitating drawing liquid |
| CN206996635U (en) * | 2017-06-15 | 2018-02-13 | 海门中科基因生物科技有限公司 | High flux pipettor |
| CN207221952U (en) * | 2017-07-11 | 2018-04-13 | 长江大学 | A kind of detachable suction head type liquid absorption device of anti-backflow |
| US20190358624A1 (en) * | 2018-05-23 | 2019-11-28 | Vistalab Technologies, Inc. | Method and apparatus for dispensing precise aliquots of liquid |
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| Publication number | Publication date |
|---|---|
| CN111097561B (en) | 2022-02-15 |
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