CN111060460A - Instrument for identifying liquid culture medium color - Google Patents
Instrument for identifying liquid culture medium color Download PDFInfo
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- CN111060460A CN111060460A CN201911389902.8A CN201911389902A CN111060460A CN 111060460 A CN111060460 A CN 111060460A CN 201911389902 A CN201911389902 A CN 201911389902A CN 111060460 A CN111060460 A CN 111060460A
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- 239000001963 growth medium Substances 0.000 title claims abstract description 23
- 238000009630 liquid culture Methods 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 52
- 230000003993 interaction Effects 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 5
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 229920001568 phenolic resin Polymers 0.000 claims description 3
- 238000010998 test method Methods 0.000 claims 1
- 230000000007 visual effect Effects 0.000 abstract description 3
- 230000006872 improvement Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000002696 acid base indicator Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/251—Colorimeters; Construction thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0256—Compact construction
- G01J3/0259—Monolithic
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0291—Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/46—Measurement of colour; Colour measuring devices, e.g. colorimeters
- G01J3/50—Measurement of colour; Colour measuring devices, e.g. colorimeters using electric radiation detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/80—Indicating pH value
Abstract
The invention relates to an instrument for identifying the color of a liquid culture medium, which comprises a detection device, light sources, a detection plate, a touch display screen, a control plate and an instrument shell, wherein the light sources are vertically fixed at two ends of the detection device and are opposite to through holes of the detection device. The touch display screen is fixed on the upper part of the instrument shell and used for displaying user interfaces and interaction, and the control panel is fixed on the bottom plate of the instrument shell. The technical scheme can test the chromaticity of the liquid culture medium, replaces the visual observation, and obviously improves the accuracy and consistency of the color interpretation of the liquid culture medium.
Description
Technical Field
The invention relates to a recognition instrument, in particular to an instrument for recognizing the color of a liquid culture medium, and belongs to the technical field of detection structure equipment.
Background
The chromogenic liquid culture medium is usually added with an acid-base indicator, and when microorganisms in the culture medium generate acid in the growth process, the pH of the culture medium is reduced, so that the acid-base indicator in the culture medium is discolored. The growth of the microorganisms in the culture medium can be judged by observing the color of the culture medium. At present, the color of the liquid medium is generally observed by naked eyes and compared with a color card. The recognition and judgment of the color change of each observer is different, and the observation result varies from person to person, resulting in inaccurate and even wrong conclusions. Therefore, it is necessary to design a color recognition device for liquid medium instead of the visual observation.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an instrument for identifying the color of the liquid culture medium.
In order to achieve the purpose, the technical scheme of the invention is that the instrument for identifying the color of the liquid culture medium is characterized by comprising a detection device, a light source, a detection plate, a touch display screen, a control plate and an instrument shell, wherein the light source is vertically fixed at two ends of the detection device and is opposite to a through hole of the detection device, the touch display screen is fixed at the upper part of the instrument shell and is used for displaying a user interface and interaction, the control plate is fixed on a bottom plate of the instrument shell, and the touch display screen is arranged at one side of the instrument shell.
As an improvement of the invention, the detection device is fixed on a bottom plate of an instrument shell, is made of black phenolic plastics, is provided with a round hole with proper depth for accommodating a sample bottle right above, and is a sample position of the instrument, and a through hole which is vertical to the side surface of the device and horizontally penetrates through the diameter of the round hole is arranged in the middle of the device and is used as a light path of the instrument.
As an improvement of the invention, the light source is a 25 x 25mm black background color printed circuit board, and the light source with the size of 5mm is fixed in the center of the circuit board.
As an improvement of the invention, the detection board is a black background printed circuit board with the thickness of 25 multiplied by 25mm, and a detection chip with the photosensitive area of 0.15 square millimeter is fixed in the center of the circuit board. This scheme needs to guarantee that one side light source is luminous, and the light that sends passes through the light path, and after shining the sample bottle that awaits measuring, the light is received to the opposite side. The light source, the detection chip, the light path and the sample bottle are aligned.
As an improvement of the invention, the control board is a control circuit board developed based on an embedded single chip microcomputer and comprises a control chip, a storage chip and necessary peripheral circuits, the control board is fixed on a bottom plate of an instrument shell, and the light source, the detection board and the touch display screen are connected with the control board through cables.
As an improvement of the invention, the instrument shell comprises a bottom plate and an upper cover, and the bottom plate and the upper cover are connected with each other through screws.
A method for detecting an instrument for color recognition of a liquid medium, the method comprising the steps of:
step 1) in the starting stage of the instrument, the instrument turns off the light source after a user takes out a sample position sample, and then the detection board is started to collect the intensity of light rays with input wavelengths of 465nm, 525nm and 615nm and record the intensity as a background value [ B ]0,G0,R0];
Step 2) then turning on the light source for a specific time by the instrument, starting the detection board to collect the intensity of light rays with input wavelengths of 465nm, 525nm and 615nm at the moment, and recording as a basic calibration value [ B ]1,G1,R1];
Step 3) when the user puts the liquid culture medium to be measured in the sample position, the instrument turns on the light source for a specific time, collects the intensity of light rays with input wavelengths of 465nm, 525nm and 615nm, and records the intensity as a detection value [ B ]t,Gt,Rt];
Step 4) detecting the value [ B ]t,Gt,Rt]Normalized to the standard detection values [ B, G, R ] according to the following formulas (1) to (3)];
Step 5) obtaining Cmax, Cmin and △ according to the following formulas (4) to (6) through standard detection values [ B, G, R ];
Cmax=max(B,G,R)……(4)
Cmin=min(B,G,R)……(5)
Δ=Cmax-Cmin……(6)
step 6) bringing the standard detection values [ B, G, R ], Cmax and △ into an equation (7) to obtain a colorimetric value H;
step 7) carrying the Cmax and △ into a formula (8) to obtain a saturation value S;
step 8) the instrument divides the color of the culture medium into a plurality of grades according to the colorimetric values through the standard sample, such as L1、L2.....Ln。
When the chromatic value H is obtained, comparing the chromatic value H with a preset threshold value, and dividing the detection result into levels closer to the preset value;
step 9) when the chroma H is less than LnThen, the saturation S is used to match a specific threshold value for further calculation, and if the saturation S is higher than the threshold value, the judgment is made as LnIf the value is less than the threshold value, the judgment is made as L1。
Compared with the prior art, the invention has the advantages that 1) the technical scheme has compact and ingenious overall structure design, can test the chromaticity of the liquid culture medium, replaces the visual observation, and obviously improves the accuracy and consistency of the color interpretation of the liquid culture medium; 2) this product cost is lower, is convenient for further popularization and application, 3) this scheme compares with traditional colourity detection device, and its simple structure only needs to use a LED light source and a control panel to fix on the instrument case bottom plate, touch display screen sets up the one side at the instrument case.
Drawings
FIG. 1 and FIG. 2 are schematic views of the overall structure of the present invention;
FIG. 3 is a schematic structural diagram of a product of the present invention;
in the figure: 1. the device comprises a tube or a bottle, 2, a detection plate, 3, a light source plate, 4, a bulb, 5, a sample position, 6, a light path, 7, an instrument shell, 8, a touch display screen, 9 and a control panel.
The specific implementation mode is as follows:
for the purpose of enhancing an understanding of the present invention, the present embodiment will be described in detail below with reference to the accompanying drawings.
Example 1: referring to fig. 1, the instrument for liquid medium color identification comprises a detection device, light sources vertically fixed at two ends of the detection device and facing to through holes of the detection device, a detection plate 2, a touch display screen 8, a control panel 9 and an instrument shell 7, wherein the touch display screen 8 is fixed at the upper part of the instrument shell 7 and used for displaying a user interface and interaction. The control panel is fixed on the instrument shell bottom plate, and the touch display screen is arranged on one side of the instrument shell.
The detection device is fixed on a bottom plate of an instrument shell and is made of black phenolic plastic, a round hole which is used for accommodating a sample bottle and has proper depth is processed right above the detection device, the round hole is a sample position of the instrument, and a through hole which is vertical to the side face of the device and horizontally penetrates through the diameter of the round hole is processed in the middle of the device to serve as a light path of the instrument.
The light source is a black background color printed circuit board with the size of 25mm multiplied by 25mm, and the light source with the size of 5mm is fixed in the center of the printed circuit board.
The detection board is a black background printed circuit board with the thickness of 25mm and 25mm, and a detection chip with the photosensitive area of 0.15 square millimeter is fixed in the center of the printed circuit board.
The control panel is a control circuit board developed based on an embedded single chip microcomputer and comprises a control chip, a storage chip and necessary peripheral circuits, the control panel is fixed on a bottom plate of an instrument shell, and the light source, the detection board and the touch display screen are connected with the control panel through cables.
The instrument shell comprises a bottom plate and an upper cover, and the bottom plate is connected with the upper cover through screws.
Example 2: referring to fig. 1, a detection method of an apparatus for liquid medium color identification, the method comprising the steps of:
step 1) in the starting stage of the instrument, the instrument turns off the light source after a user takes out a sample position sample, and then the detection board is started to collect the intensity of light rays with input wavelengths of 465nm, 525nm and 615nm and record the intensity as a background value [ B ]0,G0,R0];
Step 2) then turning on the light source for a specific time by the instrument, starting the detection board to collect the intensity of light rays with input wavelengths of 465nm, 525nm and 615nm at the moment, and recording as a basic calibration value [ B ]1,G1,R1];
Step 3) when the user puts the liquid culture medium to be measured in the sample position, the instrument turns on the light source for a specific time, collects the intensity of light rays with input wavelengths of 465nm, 525nm and 615nm, and records the intensity as a detection value [ B ]t,Gt,Rt];
Step 4) detecting the value [ B ]t,Gt,Rt]Normalized to the standard detection values [ B, G, R ] according to the following formulas (1) to (3)];
Step 5) obtaining Cmax, Cmin and △ according to the following formulas (4) to (6) through standard detection values [ B, G, R ];
Cmax=max(B,G,R)……(4)
Cmin=min(B,G,R)……(5)
Δ=Cmax-Cmin……(6)
step 6) bringing the standard detection values [ B, G, R ], Cmax and △ into an equation (7) to obtain a colorimetric value H;
step 7) carrying the Cmax and △ into a formula (8) to obtain a saturation value S;
step 8) the instrument divides the color of the culture medium into a plurality of grades according to the colorimetric values through the standard sample, such as L1、L2.....Ln。
When the chromatic value H is obtained, comparing the chromatic value H with a preset threshold value, and dividing the detection result into levels closer to the preset value;
step 9) when the chroma H is less than LnThen, the saturation S is used to match a specific threshold value for further calculation, and if the saturation S is higher than the threshold value, the judgment is made as LnIf the value is less than the threshold value, the judgment is made as L1。
It should be noted that the above-mentioned embodiments are not intended to limit the scope of the present invention, and all equivalent modifications and substitutions based on the above-mentioned technical solutions are within the scope of the present invention as defined in the claims.
Claims (7)
1. The instrument for identifying the color of the liquid culture medium comprises a detection device, a light source, a detection board, a touch display screen, a control board and an instrument shell, wherein the light source is vertically fixed at two ends of the detection device and is just opposite to a through hole of the detection device, the touch display screen is fixed on the upper portion of the instrument shell and is used for displaying a user interface and interaction, the control board is fixed on a bottom board of the instrument shell, and the touch display screen is arranged on one side of the instrument shell.
2. The apparatus as claimed in claim 1, wherein the detecting unit is fixed on the bottom plate of the casing of the apparatus, made of black phenolic plastic, and has a circular hole of proper depth for accommodating the sample bottle, which is the sample position of the apparatus, formed right above the detecting unit, and a through hole perpendicular to the side surface of the apparatus and horizontally passing through the diameter of the circular hole is formed in the middle of the detecting unit as the light path of the apparatus.
3. The apparatus as claimed in claim 2, wherein the light source is a 25 x 25mm black printed circuit board, and the light source having a size of 5mm is fixed in the center of the printed circuit board.
4. The apparatus as claimed in claim 3, wherein the sensing plate is a 25 x 25mm black printed circuit board, and the sensing chip having a light sensing area of 0.15 mm square is fixed in the center of the printed circuit board.
5. The apparatus as claimed in claim 4, wherein the control board is a control circuit board developed based on an embedded single chip microcomputer and comprises a control chip, a memory chip and necessary peripheral circuits, the control board is fixed on the bottom board of the casing of the apparatus, and the light source, the detection board and the touch display screen are connected with the control board through cables.
6. The apparatus of claim 4, wherein the apparatus housing comprises a bottom plate and a top cover, and the bottom plate and the top cover are connected to each other by screws.
7. A method of testing using the apparatus of any one of claims 1 to 6, the method comprising the steps of:
step 1) in the starting stage of the instrument, the instrument turns off the light source after a user takes out a sample position sample, and then the detection board is started to collect the intensity of light rays with input wavelengths of 465nm, 525nm and 615nm and record the intensity as a background value [ B ]0,G0,R0];
Step 2) then turning on the light source by the instrumentTiming, starting the detection board to collect the intensities of light rays with input wavelengths of 465nm, 525nm and 615nm, and recording as a basic calibration value [ B ]1,G1,R1];
Step 3) when the user puts the liquid culture medium to be measured in the sample position, the instrument turns on the light source for a specific time, collects the intensity of light rays with input wavelengths of 465nm, 525nm and 615nm, and records the intensity as a detection value [ B ]t,Gt,Rt];
Step 4) detecting the value [ B ]t,Gt,Rt]Normalized to the standard detection values [ B, G, R ] according to the following formulas (1) to (3)];
Step 5) obtaining Cmax, Cmin and △ according to the following formulas (4) to (6) through standard detection values [ B, G, R ];
Cmax=max(B,G,R) ……(4)
Cmin=min(B,G,R) ……(5)
Δ=Cmax-Cmin……(6)
step 6) bringing the standard detection values [ B, G, R ], Cmax and △ into an equation (7) to obtain a colorimetric value H;
step 7) carrying the Cmax and △ into a formula (8) to obtain a saturation value S;
step 8) the instrument divides the color of the culture medium into a plurality of grades according to the colorimetric values through the standard sample, such as L1、L2.....Ln. When the chromatic value H is obtained, comparing the chromatic value H with a preset threshold value, and dividing the detection result into levels closer to the preset value;
step 9) when the chroma H is less than LnThen, the saturation S is used to match a specific threshold value for further calculation, and if the saturation S is higher than the threshold value, the judgment is made as LnIf the value is less than the threshold value, the judgment is made as L1。
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