CN111044497B - Method for detecting drugs - Google Patents
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- CN111044497B CN111044497B CN201911341700.6A CN201911341700A CN111044497B CN 111044497 B CN111044497 B CN 111044497B CN 201911341700 A CN201911341700 A CN 201911341700A CN 111044497 B CN111044497 B CN 111044497B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Abstract
The invention provides a method for detecting drugs, belonging to the technical field of analysis and detection. The method for detecting drugs provided by the invention comprises the following steps: contacting the drug to be detected of amine hydrochloride/sulfate with alkaline test paper to react, and detecting the generated amine compound by using a fluorescence quenching detection method. The invention collects samples by using the alkaline test paper, decomposes the hydrochloride/sulfate of the amine by using the alkaline test paper, and then detects the generated amine compound by using a fluorescence quenching detection method, thereby efficiently and accurately realizing the detection of drugs.
Description
Technical Field
The invention relates to the technical field of analysis and detection, in particular to a method for detecting drugs.
Background
For dangerous people who take or carry drugs, there are often various signs of hiding the drugs, so a fast, simple and hidden drug detection method is very important. Most drugs exist stably in the form of amine hydrochloride/sulfate, the drugs are decomposed into amine compounds by a heating means in the existing method, and then the detection is carried out by adopting a fluorescence quenching detection method, specifically, the amine compounds with electron donating characteristics generated by heating decomposition are utilized to act with excited fluorescent molecules, the amine compounds transfer electrons to the fluorescent molecules, so that the fluorescence is quenched, the effect of weakening the fluorescence is achieved, and the existence of the drugs can be determined by detecting the attenuation of the fluorescence. In the method, the hydrochloride/sulfate of the amine needs to be decomposed by a heating means, and for the relatively stable hydrochloride/sulfate, the heating decomposition has the problem of relatively low efficiency, and the alarm is not easy to identify.
Disclosure of Invention
The invention aims to provide a method for detecting drugs, which is characterized in that a sample is collected by using alkaline test paper, amine hydrochloride/sulfate is decomposed by using the alkaline test paper, and then the generated amine compound is detected by using a fluorescence quenching detection method, so that the detection of the drugs can be efficiently and accurately realized.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a method for detecting drugs, which comprises the following steps:
contacting the drug to be detected of amine hydrochloride/sulfate with alkaline test paper to react, and detecting the generated amine compound by using a fluorescence quenching detection method.
Preferably, the drug to be tested comprises morphine, amphetamine or ecstasy.
Preferably, the alkaline test paper is prepared by soaking test paper in an alkaline solution; the alkaline substance in the alkaline solution comprises sodium bicarbonate, potassium bicarbonate, sodium hydroxide, potassium hydroxide, sodium carbonate or potassium carbonate, and the mass content of the alkaline substance in the alkaline solution is more than or equal to 5%.
Preferably, the alkaline test paper comprises dry test paper or wet test paper;
when the alkaline test paper is dry test paper, the alkaline solution is an alkaline substance water solution, drying is further performed after infiltration is completed, and the drying temperature is 130-200 ℃;
when the alkaline test paper is wet test paper, the alkaline solution is a mixed solution of an alkaline substance aqueous solution and ethanol, and the volume ratio of the alkaline substance aqueous solution to the ethanol is 1: (0.8 to 1.5).
Preferably, the test strip comprises a chromatographic test strip or filter paper.
Preferably, the contacting comprises:
and wiping the solid sample adhered with the drug to be detected, wiping the liquid sample containing the drug to be detected or dripping the liquid sample containing the drug to be detected on the alkaline test paper by adopting the alkaline test paper.
Preferably, after the contacting, the method further comprises: dripping an accelerant on the alkaline test paper containing the drug to be tested, wherein the accelerant comprises ethanol-water solution or ethanol-alkaline substance-water solution; the alkaline substance comprises sodium bicarbonate, potassium bicarbonate, sodium hydroxide, potassium hydroxide, sodium carbonate or potassium carbonate.
Preferably, in the ethanol-water solution and the ethanol-alkaline substance-water solution, the volume ratio of water to ethanol is independently 1: (0.8 to 1.5).
Preferably, the dropping amount of the accelerator is 2-3 mu L.
Preferably, the detection method further comprises a heat treatment when the fluorescence quenching detection method is used, wherein the heat treatment temperature is 130-200 ℃.
The invention provides a method for detecting drugs, which comprises the following steps: contacting the hydrochloride/sulfate of amine to-be-detected drug with alkaline test paper to react, and detecting the generated amine compound by using a fluorescence quenching detection method. The invention collects samples by using the alkaline test paper, decomposes the hydrochloride/sulfate of the amine by using the alkaline test paper, and then detects the generated amine compound by using a fluorescence quenching detection method, thereby efficiently and accurately realizing the detection of drugs.
Furthermore, the accelerator is adopted, so that the alkali test paper is beneficial to ionizing alkali substances on the alkali test paper, is easy to react with the hydrochloride/sulfate of the amine, and can fully decompose the hydrochloride/sulfate of the amine, thereby realizing drug detection.
Furthermore, the method is favorable for fully decomposing the hydrochloride/sulfate of the amine through heat treatment, thereby realizing drug detection.
Drawings
FIG. 1 is a flow chart of the present invention for detecting an alkaline test paper containing a drug to be detected by a fluorescence quenching detection method;
FIG. 2 is a graph showing the fluorescence test curve obtained in example 1;
FIG. 3 is a graph showing the fluorescence test curve obtained in example 2;
FIG. 4 is a graph showing the fluorescence test curve obtained in example 3;
FIG. 5 is a graph showing the fluorescence test curve obtained in example 4;
FIG. 6 is a graph showing the fluorescence test curve obtained in example 5;
FIG. 7 is a graph showing the fluorescence test curve obtained in example 6.
Detailed Description
The invention provides a method for detecting drugs, which comprises the following steps:
contacting the hydrochloride/sulfate of amine to-be-detected drug with alkaline test paper to react, and detecting the generated amine compound by using a fluorescence quenching detection method.
The invention contacts the hydrochloride/sulfate of amine to be tested with the alkaline test paper to react. The specific type of the drug to be tested is not specially limited, and the drug to be tested can be morphine, methamphetamine hydrochloride or dancing outreach; in the embodiment of the invention, the detection is carried out by taking phenethylamine hydrochloride as a drug simulator.
In the invention, the alkaline test paper is preferably prepared by soaking the test paper in an alkaline solution; the alkaline substance in the alkaline solution preferably comprises sodium bicarbonate, potassium bicarbonate, sodium hydroxide, potassium hydroxide, sodium carbonate or potassium carbonate, and more preferably sodium carbonate or potassium carbonate; the mass content of the alkaline substance in the alkaline solution is preferably not less than 5%, more preferably not less than 10%, further preferably not less than 20%, and still further preferably a saturated solution; the test paper preferably comprises chromatographic test paper or filter paper, and more preferably chromatographic test paper; the size of the test paper is not specially limited, and the test paper can meet the detection requirement, and in the embodiment of the invention, the size of the test paper is specifically 70mm × 20 mm.
In the present invention, the alkaline test paper may be dry test paper or wet test paper, specifically:
when the alkaline test paper is dry test paper, the alkaline solution is preferably an alkaline substance aqueous solution; the step of drying is also preferably included after the infiltration is finished, and the drying temperature is preferably 130-200 ℃, more preferably 140-160 ℃, and further preferably 150 ℃; the drying time is not specially limited, and the soaked alkaline test paper can be fully dried; after drying, the obtained dry test paper is preferably placed in a packaging bag for packaging and standby.
When the alkaline test paper is a wet test paper, the alkaline solution is preferably a mixed solution of an alkaline substance aqueous solution and ethanol, and the volume ratio of the alkaline substance aqueous solution to the ethanol is preferably 1: (0.8 to 1.5), more preferably 1: 1; the wet test paper obtained after soaking is preferably placed in a packaging bag containing a small amount of alkaline solution (namely a mixed solution of an alkaline substance aqueous solution and ethanol) for packaging for later use, and the addition amount of the alkaline solution in the packaging bag is not particularly limited, so that the wet test paper can be kept in a wet state. In the invention, the solvent in the alkaline solution is water and ethanol when preparing the wet test paper, wherein, water can fully ionize alkaline substances and is easier to react with the hydrochloride/sulfate of amine to-be-tested drugs, and ethanol can quickly volatilize the solvent in the wet test paper to quickly dry the wet test paper for detection (the test paper needs to be ensured to be dry during detection, if the test paper is wet, steam can be generated during detection to influence fluorescence change).
After the alkaline test paper is prepared, the drug to be tested of amine hydrochloride/sulfate is contacted with the alkaline test paper to react. The mode of contacting the drug to be tested with the alkaline test paper is not particularly limited, and the alkaline test paper is preferably used for wiping a solid sample adhered with the drug to be tested, wiping a liquid sample containing the drug to be tested or dripping the liquid sample containing the drug to be tested on the alkaline test paper. The method provided by the invention is simple to operate, and the collection of the sample can be rapidly and covertly realized by using the method of wiping by using the alkaline test paper; after a drug to be detected is contacted with the alkaline test paper, an alkaline substance on the alkaline test paper reacts with hydrochloride/sulfate of amine to generate a corresponding amine compound, and the amine compound has an electron donating property and can transfer electrons to fluorescent molecules in an excited state, so that electrons excited by light in the fluorescent molecules cannot directly jump back to an original ground state orbit, fluorescence quenching is caused, and rapid detection of the drug is realized.
In the present invention, the solid sample adhered with the drug to be tested may be hair, skin, clothes, etc. of a drug addict, which is not particularly limited in the present invention; the liquid sample containing the drug to be tested can be drinking water, wine and the like, and the invention is not particularly limited in this respect. In the embodiment of the invention, the ethanol solution of the drug to be tested is dripped on the alkaline test paper to realize the contact of the ethanol solution and the alkaline test paper, so that the feasibility of the method is verified. In the invention, the concentration of the ethanol solution of the drug to be tested is preferably 10 ng/muL, and the dropping amount is preferably 1 muL.
In the invention, when the alkaline test paper is dry test paper, the dry test paper can be used for subsequent treatment after wiping a solid sample adhered with a drug to be detected; after the dry test paper is wiped with a liquid sample containing a drug to be tested or the liquid sample containing the drug to be tested is dripped on the dry test paper, the dry test paper is preferably naturally dried at room temperature and then is subjected to subsequent treatment. When the alkaline test paper is wet test paper, the wet test paper is used for wiping a solid sample adhered with a drug to be tested, wiping a liquid sample containing the drug to be tested or dripping the liquid sample containing the drug to be tested on the alkaline test paper, then naturally airing the alkaline test paper preferably at room temperature, and then carrying out subsequent treatment.
In the present invention, the post-contact preferably further comprises: dripping an accelerant on the alkaline test paper containing the drug to be tested; the dropping position of the accelerant is preferably consistent with the contact position of the drug to be tested and the alkaline test paper. In the present invention, the accelerator preferably comprises an ethanol-water solution or an ethanol-alkaline substance-water solution; the basic substance preferably comprises sodium bicarbonate, potassium bicarbonate, sodium hydroxide, potassium hydroxide, sodium carbonate or potassium carbonate, more preferably sodium carbonate or potassium carbonate. In the present invention, in the ethanol-aqueous solution and the ethanol-alkaline substance-aqueous solution, the volume ratio of water to ethanol is preferably 1: (0.8 to 1.5), more preferably 1: 1; the mass content of the alkaline substance in the ethanol-alkaline substance-water solution is not particularly limited, and can be up to a saturated state. In the invention, the dropping amount of the accelerator is preferably 2-3 mu L. The accelerant is favorable for ionizing alkaline substances on the alkaline test paper and is easy to react with the drug to be detected, specifically, water in the accelerant can fully ionize the alkaline substances and is easy to act with the hydrochloride/sulfate of amine to be detected, and the ethanol can quickly volatilize the solvent and facilitate subsequent detection. In the embodiment of the invention, when the alkaline test paper is a wet test paper, the alkaline test paper contains an alkaline solution (namely a mixed solution of an alkaline substance aqueous solution and ethanol) and can play a role of an accelerant, and the accelerant does not need to be additionally added dropwise; and when the alkaline test paper is dry test paper, dripping the accelerant, preferably naturally airing at room temperature, and then carrying out subsequent treatment.
In the invention, the detection of the generated amine compound by using a fluorescence quenching detection method can be carried out at room temperature, i.e. no additional heating or cooling is needed; in order to further enable the alkaline substance on the alkaline test paper to fully react with the drug to be detected and promote the decomposition of the drug to be detected, the fluorescence quenching detection method preferably further comprises heat treatment when the fluorescence quenching detection method is used for detection, and the heat treatment temperature is preferably 140-160 ℃, and more preferably 150 ℃.
The specific steps and the equipment used in the fluorescence quenching detection method are not particularly limited, and the steps and the equipment known to those skilled in the art can be used. In the embodiment of the invention, a perylene derivative (a compound with a structure shown in formula I) is specifically used as a fluorescent material, the fluorescent material is coated on the inner wall of a quartz tube (specifically, the fluorescent material is coated at a position corresponding to a fluorescence detector of the quartz tube, and the whole inner wall of the quartz tube is not required to be coated), the quartz tube is placed in a dark environment, an alkaline test paper containing a drug to be detected or an alkaline test paper containing the drug to be detected and an accelerant (if the test paper is in a dry state, natural airing is not required, if the test paper is used for wiping a solid sample adhered with the drug to be detected by using a dry test paper to realize sample collection, the accelerant is not further dripped, the alkaline test paper is in a dry state, natural airing is not required, and in other cases, after the accelerant is sampled or dripped, the alkaline test paper is in a wet state, natural airing is required) is placed in a clamping piece of a drug detection instrument (heat treatment or natural airing is selected according to need) The thermal treatment is not performed, if the thermal treatment is performed, the clamping piece is preset to the temperature required by the thermal treatment, and the temperature is always kept in the detection process), the drug to be detected reacts with the alkaline substance of the alkaline test paper to generate a corresponding amine compound, the amine compound is subjected to the air suction action of a pump in the drug detection instrument, the fluorescent material emits light through a quartz tube with the inner wall coated with the fluorescent material by using an excitation light source (the excitation light source is opened before the test starts), the amine compound reacts with the fluorescent molecules in the excitation state to quench the light emission of the partial fluorescent molecules, so that the fluorescence detected by a fluorescence spectrometer in the drug detection instrument is weakened, and the qualitative detection of the drug is realized (the flow diagram of the detection method is shown in fig. 1, and the excitation light source is not shown in fig. 1).
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Fully soaking the chromatographic test paper (70mm multiplied by 20mm) in a saturated sodium carbonate solution, placing the obtained wet chromatographic test paper on a heating platform at 150 ℃ for drying to obtain dry test paper, and placing the dry test paper in a packaging bag for packaging for later use.
Selecting phenylethylamine hydrochloride as a drug simulator for testing, specifically, preparing an ethanol solution of the phenylethylamine hydrochloride with the concentration of 10 ng/mu L as a sample to be tested, using a perylene derivative (a compound with a structure shown in formula I) as a fluorescent material, coating the fluorescent material on the inner wall of a quartz tube, placing the quartz tube in a dark environment, dropwise adding 1 mu L of the sample to be tested on dry test paper, naturally drying the test paper at room temperature, placing the test paper in a clamping piece (unheated) of a drug detection instrument (the sample to be tested reacts with sodium carbonate on the dry test paper to generate phenylethylamine), subjecting the phenylethylamine generated by the sample to be tested to the suction action of a pump in the drug detection instrument, enabling the fluorescent material to emit light by using an excitation light source (the excitation light source is opened before the test is started) through the quartz tube coated with the fluorescent material on the inner wall, and enabling the amine compound to act with fluorescent molecules in an excitation state, the fluorescence of the fluorescence molecule in the drug detection instrument is weakened by quenching the luminescence of part of the fluorescence molecules, the fluorescence test curve is shown in figure 2, and the obtained fluorescence test curve is obviously attenuated as shown in figure 2, which shows that the invention can decompose the phenylethylamine hydrochloride by using the alkaline test paper, thereby realizing the drug detection.
Example 2
The detection was performed using dry test paper with phenylethylamine hydrochloride according to the method of example 1, except that 1 μ L of the sample to be detected was dropped on the dry test paper, and after naturally drying at room temperature, the test paper was placed in a clip of a drug detection instrument (the clip was preheated to 150 ℃ first, and the temperature was maintained throughout the subsequent detection process), and then the subsequent operations were performed. The obtained fluorescence test curve is shown in fig. 3, and as can be seen from fig. 3, the fluorescence test curve has obvious attenuation, which shows that the invention utilizes the alkaline test paper and the heating means, and can promote the decomposition of the phenylethylamine hydrochloride, thereby realizing drug detection.
Example 3
The detection was performed using dry test paper with phenylethylamine hydrochloride according to the method of example 1, except that 1 μ L of the sample to be detected was dropped on the dry test paper, then 2 μ L of the accelerator (obtained by mixing a saturated sodium carbonate solution with an equal volume of ethanol) was dropped on the drop position of the sample to be detected, and after naturally drying the sample at room temperature, the obtained dry test paper was placed in a clip (not heated) for subsequent operations. The obtained fluorescence test curve is shown in fig. 4, and as can be seen from fig. 4, the fluorescence test curve has obvious attenuation, which shows that the alkaline test paper is utilized, the accelerant is dripped after the sample to be tested is dripped, so that sodium carbonate on the alkaline test paper is ionized, the reaction with phenethylamine hydrochloride is easier, the decomposition of the phenethylamine hydrochloride can be promoted, and the drug detection is realized.
Example 4
The detection is carried out by using dry test paper for detecting phenylethylamine hydrochloride according to the method of the embodiment 1, except that 1 mu L of a sample to be detected is dripped on the dry test paper, then 2 mu L of an accelerant (the accelerant is obtained by mixing saturated sodium carbonate solution and ethanol with the same volume) is dripped on the dripping position of the sample to be detected, the mixture is naturally dried at room temperature, then the mixture is placed in a clamping piece (the clamping piece is preheated to 150 ℃ at first and is kept at the temperature all the time in the subsequent detection process) of a drug detection instrument, and then the subsequent operation is carried out. The obtained fluorescence test curve is shown in fig. 5, and as can be seen from fig. 5, the fluorescence test curve has obvious attenuation, which shows that the alkaline test paper is utilized, the accelerant is dripped after the sample to be tested is dripped, so that sodium carbonate on the alkaline test paper is ionized, the reaction with phenethylamine hydrochloride is easier, and meanwhile, the dissolution of the phenethylamine hydrochloride can be promoted by matching with a heating means, so that drug detection is realized.
Example 5
Mixing a saturated potassium carbonate solution with ethanol with the same volume to obtain a mother solution; and (3) fully soaking chromatographic test paper (70mm multiplied by 20mm) in the mother liquor to obtain wet test paper, and placing the wet test paper in a packaging bag containing a small amount of mother liquor for packaging for later use.
1 mul of a sample to be detected (an ethanol solution of phenethylamine hydrochloride with the concentration of 10 ng/mul) is dripped on the wet test paper, the wet test paper is naturally dried at room temperature, and then the wet test paper is placed in a clamping piece (without heating), and then the subsequent detection step is carried out according to the method of the embodiment 1. The obtained fluorescence test curve is shown in fig. 6, and as can be seen from fig. 6, the fluorescence test curve has obvious attenuation, which shows that the method utilizes the mother liquor on the alkaline test paper, is favorable for ionizing sodium carbonate on the alkaline test paper, is easy to react with phenethylamine hydrochloride, can promote the decomposition of the phenethylamine hydrochloride, and further realizes drug detection.
Example 6
The detection was performed using wet paper for phenylethylamine hydrochloride according to the method of example 5, except that 1 μ L of the sample to be detected was dropped on the wet paper and naturally dried at room temperature, and then placed in a clip of a drug detection instrument (the clip was preheated to 150 ℃ first and kept at this temperature throughout the subsequent detection process), followed by the subsequent operation. The obtained fluorescence test curve is shown in fig. 7, and as can be seen from fig. 7, the fluorescence test curve has obvious attenuation, which shows that the invention utilizes the mother liquor on the alkaline test paper, is beneficial to ionizing sodium carbonate on the alkaline test paper, is easier to react with phenethylamine hydrochloride, and can promote the decomposition of the phenethylamine hydrochloride by matching with a heating means, thereby realizing drug detection.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. A method for detecting drugs, comprising the steps of:
contacting the hydrochloride/sulfate of amine to-be-detected drug with alkaline test paper to react, and detecting the generated amine compound by using a fluorescence quenching detection method; the alkaline test paper is prepared by soaking test paper in an alkaline solution; the alkaline substance in the alkaline solution is sodium bicarbonate, potassium bicarbonate, sodium hydroxide, potassium hydroxide, sodium carbonate or potassium carbonate;
after the contacting, the method further comprises the following steps: dripping an accelerant on the alkaline test paper containing the drug to be tested, wherein the accelerant is ethanol-water solution or ethanol-alkaline substance-water solution; the ethanol-alkaline substance-aqueous solution contains alkaline substance such as sodium bicarbonate, potassium bicarbonate, sodium hydroxide, potassium hydroxide, sodium carbonate or potassium carbonate.
2. The method of claim 1, wherein the drug to be tested comprises morphine, methamphetamine or ecstasy.
3. The method according to claim 1, wherein the alkaline solution contains alkaline substances in an amount of 5% by mass or more.
4. The method of claim 3, wherein the alkaline dipstick comprises a dry dipstick or a wet dipstick;
when the alkaline test paper is dry test paper, the alkaline solution is an alkaline substance water solution, drying is further performed after infiltration is completed, and the drying temperature is 130-200 ℃;
when the alkaline test paper is a wet test paper, the alkaline solution is a mixed solution of an alkaline substance aqueous solution and ethanol, and the volume ratio of the alkaline substance aqueous solution to the ethanol is 1: (0.8 to 1.5).
5. The method of claim 3 or 4, wherein the test strip comprises a chromatographic test strip or filter paper.
6. The method of claim 1, wherein the contacting comprises:
and wiping the solid sample adhered with the drug to be detected, wiping the liquid sample containing the drug to be detected or dripping the liquid sample containing the drug to be detected on the alkaline test paper by adopting the alkaline test paper.
7. The method according to claim 1, wherein the ethanol-water solution and the ethanol-alkaline substance-water solution have a volume ratio of water to ethanol of 1: (0.8-1.5).
8. The method according to claim 7, wherein the amount of the accelerator is 2 to 3. mu.L.
9. The method according to any one of claims 1 and 6 to 8, wherein the detection by the fluorescence quenching detection method further comprises a heat treatment, and the temperature of the heat treatment is 130 to 200 ℃.
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| CN113466442B (en) * | 2021-07-29 | 2023-04-07 | 广州蓝勃生物科技有限公司 | Drug trace analyzer |
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