CN111040029B - Sample diluent preparation device and operation method - Google Patents
Sample diluent preparation device and operation method Download PDFInfo
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- CN111040029B CN111040029B CN201911350176.9A CN201911350176A CN111040029B CN 111040029 B CN111040029 B CN 111040029B CN 201911350176 A CN201911350176 A CN 201911350176A CN 111040029 B CN111040029 B CN 111040029B
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- sleeve
- fastening
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- mounting
- centrifugal machine
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
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- Gastroenterology & Hepatology (AREA)
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- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Centrifugal Separators (AREA)
Abstract
The sample diluent preparation device comprises a centrifuge, a positioning plate, a mounting sleeve, a fastening sleeve, a sliding ring and a supporting spring, wherein a supporting frame is arranged outside the lower portion of the centrifuge, the two sides of the supporting frame are connected with the positioning plate through mounting shafts, the upper end of the positioning plate is clamped on the centrifuge, the mounting sleeve is connected to the outer side of the centrifuge, the fastening sleeve is arranged on the upper side of the mounting sleeve and slides on the centrifuge, the upper end of the mounting sleeve is connected with the fastening sleeve through threads, the fastening nail is connected to the outer side of the fastening sleeve and is sequentially connected with the fastening sleeve and the positioning plate, the mounting sleeve is connected with the lower end of the supporting spring, the sliding ring is connected to the upper end of the supporting spring and is clamped in the fastening sleeve, the sliding ring rotates relative to the fastening sleeve, the ball sleeve is connected to the upper side of the sliding ring, and balls are connected to the inside of the ball sleeve.
Description
Technical Field
The invention relates to the field of sample diluents, in particular to a sample diluent preparation device and an operation method.
Background
Bovine serum albumin is the important composition of antibody sample diluent, and need utilize centrifuge in bovine serum albumin's preparation process, however current sample diluent preparation facilities structure is unstable, easily takes place to rock because of centrifugal force in the preparation process.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a sample diluent preparation device with a stable structure and an operation method.
The purpose of the invention is realized by the following technical scheme:
the sample diluent preparation device comprises a centrifugal machine, a positioning plate, an installation sleeve, a fastening sleeve, a sliding ring and a supporting spring, wherein a supporting frame is arranged outside the lower portion of the centrifugal machine, the two sides of the supporting frame are connected with the positioning plate through installation shafts, the upper end of the positioning plate is clamped on the centrifugal machine, the installation sleeve is connected to the outer side of the centrifugal machine, the fastening sleeve is arranged on the upper side of the installation sleeve, the fastening sleeve slides on the centrifugal machine, the upper end of the installation sleeve is connected with the fastening sleeve through threads, the fastening nail is connected to the outer side of the fastening sleeve, the fastening nail is sequentially connected with the fastening sleeve and the positioning plate, the lower end of the supporting spring is connected with the lower end of the supporting spring, the sliding ring is connected to the upper end of the supporting spring and clamped in the fastening sleeve, the sliding ring rotates relative to the fastening sleeve, the upper side of the sliding ring is connected with the ball sleeve, the ball sleeve is internally connected with a ball, and the ball rolls in the fastening sleeve.
The support frame both sides have the locating lever, the locating lever sets up at the locating plate inboard, the locating plate downside has the constant head tank, the locating lever passes the constant head tank and connects the position sleeve, the position sleeve card is in the constant head tank.
Has the advantages that:
the positioning rod passes through the positioning groove and is connected with the positioning sleeve, so that the positioning plate can be fixed perpendicular to the support frame. The locating plate upper end card makes centrifuge can set up perpendicularly relative to the support frame on centrifuge for centrifuge can be accurate in the ascending fixed position of vertical side. The binding nail connects gradually adapter sleeve, installation cover through the screw, makes centrifuge, locating plate, installation cover, adapter sleeve stable in structure relatively, and the centrifuge position receives the restriction, and then makes centrifuge be difficult for taking place to rock under centrifugal force's influence. The upper end of the mounting sleeve is connected with the fastening sleeve through threads, so that the mounting sleeve and the fastening sleeve are relatively stable in structure, and the fastening nail is convenient to fix. When installation cover, adapter sleeve helicitic texture separation, supporting spring upwards supports the adapter sleeve, makes adapter sleeve and position sleeve separation for centrifuge position is unrestricted, can carry out normal operation, and is rational in infrastructure. The ball rolls in the fastening sleeve, so that the sliding ring slides stably in the fastening sleeve and is not easy to clamp.
Drawings
Fig. 1 is a schematic structural diagram of a sample diluent preparation apparatus according to the present invention.
Fig. 2 is a partially enlarged view of fig. 1 according to the present invention.
Fig. 3 is a partially enlarged view of fig. 2 according to the present invention.
Detailed Description
The invention is explained in more detail below with reference to the figures and examples:
the utility model provides a sample diluent preparation facilities, includes centrifuge 1, locating plate 4, mounting sleeve 8, adapter sleeve 9, slip ring 10, supporting spring 13, centrifuge 1 lower part outside has support frame 2, 2 both sides of support frame are through installing axle 3 connection locating plate 4, 4 upper end cards of locating plate are on centrifuge 1, mounting sleeve 8 is connected in the centrifuge 1 outside, adapter sleeve 9 sets up at 8 upsides of mounting sleeve, adapter sleeve 9 slides on centrifuge 1, the mounting sleeve 8 upper end is through threaded connection adapter sleeve 9, adapter pin 14 is connected in the adapter sleeve 9 outside, adapter sleeve 9, locating plate 4 are connected in proper order to adapter pin 14, mounting sleeve 8 connects supporting spring 13 lower extreme, supporting spring 13 upper end connection slip ring 10, slip ring 10 cards is in adapter sleeve 9, slip ring 10 rotates relative adapter sleeve 9, slip ring 10 upside connection ball cover 11, ball cover 11 internal connection ball 12, ball 12 rolls in adapter sleeve 9.
Positioning rods 6 are arranged on two sides of the support frame 2, the positioning rods 6 are arranged on the inner sides of the positioning plates 4, positioning grooves 5 are formed in the lower sides of the positioning plates 4, the positioning rods 6 penetrate through the positioning grooves 5 to be connected with positioning sleeves 7, and the positioning sleeves 7 are clamped in the positioning grooves 5.
The positioning rod 6 passes through the positioning groove 5 and is connected with the positioning sleeve 7, so that the positioning plate 4 can be fixed perpendicular to the support frame 2. The upper end of the positioning plate 4 is clamped on the centrifuge 1, so that the centrifuge 1 can be vertically arranged relative to the support frame 2, and the centrifuge 1 can be accurately fixed and positioned in the vertical direction. The fastening nail 14 is sequentially connected with the fastening sleeve 9 and the mounting sleeve 8 through threads, so that the centrifugal machine 1, the positioning plate 4, the mounting sleeve 8 and the fastening sleeve 9 are relatively stable in structure, the position of the centrifugal machine 1 is limited, and the centrifugal machine 1 is not prone to shaking under the influence of centrifugal force. The upper end of the mounting sleeve 8 is connected with the fastening sleeve 9 through threads, so that the mounting sleeve 8 and the fastening sleeve 9 are relatively stable in structure, and the fastening nail 14 can be fixed conveniently. When the mounting sleeve 8, the 9 helicitic texture of adapter sleeve separated, supporting spring 13 upwards supported adapter sleeve 9, made adapter sleeve 9 and the separation of position sleeve 7 for centrifuge 1 position is unrestricted, can normally operate, and is rational in infrastructure. The balls 12 roll in the fastening sleeve 9, so that the sliding ring 10 slides stably in the fastening sleeve 9 and is not easy to be blocked.
A method of operating a sample diluent preparation device comprising the steps of:
firstly, adjusting a centrifuge 1 to a vertical position, turning a positioning plate 4 inwards, clamping a positioning rod 6 in a positioning groove 5, fixing a positioning sleeve 7 on the positioning groove 5 through threads, and clamping the lower part of the positioning sleeve 7 in the positioning groove 5;
second step, promote adapter sleeve 9 downwards, slip ring 10 compresses supporting spring 13 downwards, and the rotatory adapter sleeve 9 of rethread meshes adapter sleeve 9 and 8 helicitic texture of installation cover, and slip ring 10 rotates relative adapter sleeve 9 simultaneously:
and thirdly, screwing the fastening nail 14 inwards to enable the fastening nail 14 to be sequentially connected with the fastening sleeve 9 and the positioning plate 4, and fixing the positioning plate 4 and the fastening sleeve 9 to each other to finish the fixation of the centrifuge 1.
Claims (2)
1. A sample diluent preparation device is characterized by comprising a centrifugal machine, a positioning plate, a mounting sleeve, a fastening sleeve, a sliding ring and a supporting spring, wherein a supporting frame is arranged outside the lower part of the centrifugal machine, two sides of the supporting frame are connected with the positioning plate through mounting shafts, the upper end of the positioning plate is clamped on the centrifugal machine, the outer side of the centrifugal machine is connected with the mounting sleeve, the outer side of the centrifugal machine is connected with the fastening sleeve, the fastening sleeve is arranged on the upper side of the mounting sleeve, the fastening sleeve slides on the centrifugal machine, the upper end of the mounting sleeve is connected with the fastening sleeve through threads, the outer side of the fastening sleeve is connected with a fastening nail, the fastening nail is sequentially connected with the fastening sleeve and the positioning plate, the mounting sleeve is connected with the lower end of the supporting spring, the upper end of the supporting spring is connected with the sliding ring, the sliding ring is clamped in the fastening sleeve, the sliding ring rotates relative to the fastening sleeve, the upper side of the sliding ring is connected with the ball sleeve, the ball sleeve is connected with the ball in the ball sleeve, and rolls in the fastening sleeve; positioning rods are arranged on two sides of the support frame, the positioning rods are arranged on the inner sides of the positioning plates, positioning grooves are formed in the lower sides of the positioning plates, the positioning rods penetrate through the positioning grooves and are connected with positioning sleeves, and the positioning sleeves are clamped in the positioning grooves; the fixing nail is sequentially connected with the fastening sleeve and the mounting sleeve through threads, so that the centrifugal machine, the positioning plate, the mounting sleeve and the fastening sleeve are relatively stable in structure, the position of the centrifugal machine is limited, and the centrifugal machine is not easy to shake under the influence of centrifugal force; when the mounting sleeve and the fastening sleeve are separated in a threaded structure, the supporting spring upwards supports the fastening sleeve to separate the fastening sleeve from the positioning sleeve, so that the position of the centrifuge is not limited, and normal operation can be performed.
2. The method of claim 1, wherein the sample diluent preparing apparatus comprises:
firstly, adjusting a centrifuge to a vertical position, turning a positioning plate inwards, clamping a positioning rod in a positioning groove, fixing a positioning sleeve on the positioning groove through threads, and clamping the lower part of the positioning sleeve in the positioning groove;
second step, promote the adapter sleeve downwards, the slip ring compresses supporting spring downwards, and the rotatory adapter sleeve of rethread meshes adapter sleeve and installation cover helicitic texture, and the relative adapter sleeve of slip ring rotates simultaneously:
and thirdly, screwing the fastening nails inwards to enable the fastening nails to be sequentially connected with the fastening sleeves and the positioning plate, and fixing the positioning plate and the fastening sleeves to each other to finish the fixation of the centrifuge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911350176.9A CN111040029B (en) | 2019-12-24 | 2019-12-24 | Sample diluent preparation device and operation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911350176.9A CN111040029B (en) | 2019-12-24 | 2019-12-24 | Sample diluent preparation device and operation method |
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| Publication Number | Publication Date |
|---|---|
| CN111040029A CN111040029A (en) | 2020-04-21 |
| CN111040029B true CN111040029B (en) | 2023-04-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201911350176.9A Active CN111040029B (en) | 2019-12-24 | 2019-12-24 | Sample diluent preparation device and operation method |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207887344U (en) * | 2017-12-27 | 2018-09-21 | 佳木斯大学 | Novel centrifuge attachment device |
| CN207945421U (en) * | 2017-12-28 | 2018-10-09 | 佳木斯大学 | A kind of water-bath trough fixation device |
| CN208138214U (en) * | 2018-04-26 | 2018-11-23 | 吴宝贤 | Novel oilfield collection is defeated to fix device with centrifugal pump |
| CN208378761U (en) * | 2018-05-14 | 2019-01-15 | 天津市正江高科技有限公司 | Extract seralbumin reactor |
| CN208383556U (en) * | 2018-05-22 | 2019-01-15 | 朱圭娜 | A kind of clinical laboratory's sample separator |
| CN208627560U (en) * | 2018-05-14 | 2019-03-22 | 天津市正江高科技有限公司 | Extract seralbumin centrifuge |
| CN208670450U (en) * | 2018-07-17 | 2019-03-29 | 苑欣华 | Novel mechanical equipment firm banking |
| CN209205563U (en) * | 2018-10-26 | 2019-08-06 | 陈云玲 | Novel protein separation horizontal type centrifuge fixes device |
-
2019
- 2019-12-24 CN CN201911350176.9A patent/CN111040029B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207887344U (en) * | 2017-12-27 | 2018-09-21 | 佳木斯大学 | Novel centrifuge attachment device |
| CN207945421U (en) * | 2017-12-28 | 2018-10-09 | 佳木斯大学 | A kind of water-bath trough fixation device |
| CN208138214U (en) * | 2018-04-26 | 2018-11-23 | 吴宝贤 | Novel oilfield collection is defeated to fix device with centrifugal pump |
| CN208378761U (en) * | 2018-05-14 | 2019-01-15 | 天津市正江高科技有限公司 | Extract seralbumin reactor |
| CN208627560U (en) * | 2018-05-14 | 2019-03-22 | 天津市正江高科技有限公司 | Extract seralbumin centrifuge |
| CN208383556U (en) * | 2018-05-22 | 2019-01-15 | 朱圭娜 | A kind of clinical laboratory's sample separator |
| CN208670450U (en) * | 2018-07-17 | 2019-03-29 | 苑欣华 | Novel mechanical equipment firm banking |
| CN209205563U (en) * | 2018-10-26 | 2019-08-06 | 陈云玲 | Novel protein separation horizontal type centrifuge fixes device |
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| Publication number | Publication date |
|---|---|
| CN111040029A (en) | 2020-04-21 |
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Effective date of registration: 20230912 Address after: 300000 West of Lanjiazhuang Village, Dajianchang Town, Wuqing District, Tianjin City Patentee after: TIANJIN KANGERKE BIOSCIENCE Co.,Ltd. Address before: No. 2 Guotai Road, Dajianchang Town, Wuqing District, Tianjin, 300000 Patentee before: TIANJIN QITEER BIOTECHNOLOGY Co.,Ltd. |