CN111039393B - Filler for assisting formation of fungus mycelium pellets - Google Patents
Filler for assisting formation of fungus mycelium pellets Download PDFInfo
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- CN111039393B CN111039393B CN201911144982.0A CN201911144982A CN111039393B CN 111039393 B CN111039393 B CN 111039393B CN 201911144982 A CN201911144982 A CN 201911144982A CN 111039393 B CN111039393 B CN 111039393B
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- 239000008188 pellet Substances 0.000 title claims abstract description 30
- 239000000945 filler Substances 0.000 title claims abstract description 28
- 241000233866 Fungi Species 0.000 title claims abstract description 16
- 230000015572 biosynthetic process Effects 0.000 title claims description 8
- 229920001903 high density polyethylene Polymers 0.000 claims description 6
- 239000004700 high-density polyethylene Substances 0.000 claims description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 7
- 238000000855 fermentation Methods 0.000 abstract description 6
- 230000004151 fermentation Effects 0.000 abstract description 6
- 238000010008 shearing Methods 0.000 abstract description 4
- 230000035755 proliferation Effects 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 238000005243 fluidization Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 9
- 230000006835 compression Effects 0.000 description 6
- 238000007906 compression Methods 0.000 description 6
- 239000010865 sewage Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000012360 testing method Methods 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 229920000049 Carbon (fiber) Polymers 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000004917 carbon fiber Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/10—Packings; Fillings; Grids
- C02F3/109—Characterized by the shape
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biological Treatment Of Waste Water (AREA)
Abstract
The invention discloses a filler for assisting fungus mycelium pellets to form, which has a regular dodecahedron shape in whole, and comprises a regular dodecahedron shell and a conical thorn structure on the outer surface of the shell, wherein the regular dodecahedron shell is a hollow structure consisting of round edge lines, the conical thorn structure consists of a plurality of conical thorns, and each round edge line is provided with a conical thorn perpendicular to the single-sided direction of the regular pentagon of the regular dodecahedron. The invention can realize good fluidization by using lower energy consumption, and the filler can provide more shearing force and growth and proliferation space of fungus hypha during culture of a shaking table or a fermentation tank, thereby being beneficial to the attachment growth of fungi between the external conical spines of the right twelve surfaces.
Description
Technical Field
The invention belongs to the technical field of fillers used for sewage treatment in the environment-friendly industry, and particularly relates to a filler for assisting mycelium pellet formation.
Background
Mycelium pellet is a microorganism aggregate formed under a certain culture condition, has the characteristics of strong metabolic capacity, large pollutant adsorption amount and the like, and also has the characteristics of high biological activity, high sedimentation speed, easiness in solid-liquid separation and the like. In recent years, the technology has become a research hotspot in the fields of biological engineering, environmental protection and the like at home and abroad.
Mycelium pellet is a special fermentation form of filamentous fungi, namely, under the conditions of sufficient oxygen and nutrition, low viscosity of culture medium and proper shearing hydraulic strength, germinated filamentous fungi spores form mycelium, which are intertwined with each other to form spheroid-like mycelium aggregates with compact surfaces and loose interiors. The mycelium pellet is favorable for mass transfer and oxygen transfer due to the structural characteristics of multiple holes, large surface area and multiple netlike gaps, and is a novel microbial material with bioactivity and adsorption and carrier characteristics. The mycelium pellet also has the advantages of good sedimentation performance, compact internal form and structural characteristics, safety, no toxicity and the like, and also has strong impact load resistance. Meanwhile, the mycelium pellet has excellent adaptability and can effectively cope with complex and changeable wastewater environments, so that the mycelium pellet is a typical environment-friendly biological material. However, in industrial applications, mycelium pellets are used as biological carriers in water treatment, and the structural stability of mycelium pellets is related to the service life of the carrier, the treatment effect and the economic benefit. When the actual wastewater is treated, the structural stability is poor; the forming time is long; the problem of poor solid-liquid separation effect due to suspension of the mycelium pellets caused by different solution densities is that mycelium pellets are relatively deficient in carrier application compared with other types of carriers, and the practical application value of the mycelium pellets is affected. In industrial applications, the rapid culture and stable operation of mycelium pellets is still a major bottleneck restricting the application of the material. At present, the balling of mycelium pellets is still in a shaking culture manner of a shaking table without adding a filler and a culture manner of a fermentation tank, and auxiliary materials for promoting mycelium pellet formation have not been reported and applied yet.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a filler formed by auxiliary mycelium pellets for sewage treatment.
The technical scheme provided by the invention is as follows.
The filler formed by the auxiliary mycelium pellets for sewage treatment is characterized by having a regular dodecahedron shape as a whole, comprising a regular dodecahedron shape shell and a conical thorn structure on the outer surface of the shell, wherein the conical thorn structure is formed by a plurality of conical thorns, and each conical thorn is perpendicular to a regular pentagon single face of the regular dodecahedron shape shell.
Further, the diameter of the inscribed sphere of the regular dodecahedron-shaped shell is 5 mm, and the diameters of the round edge lines forming the regular dodecahedron-shaped shell are all 0.2 mm; the height of the conical spines is 3 mm, and the diameter of the bottom surface of the conical spines is 0.2 mm.
Further, 30 circular cones are uniformly distributed on the five circular ridge lines of the regular dodecahedron single face.
Further, the material is high-density polyethylene with the density of 0.941-0.960 g/cm 3 。
The beneficial effects of the invention are that.
The invention has the appearance of natural color of high-density polyethylene, and the density of the natural color is 0.941-0.960 g/cm 3 Can utilize lower energy consumptionGood fluidization is realized; the whole shell is a hollow regular dodecahedron-shaped structure, and a conical thorn is arranged on a round ridge line of the regular dodecahedron shell; the method is applied to shake culture or fermentation tank culture, is beneficial to winding fungus mycelia on the surface of the fungus mycelia, provides more shearing force and mycelia proliferation space, and is beneficial to the attached growth of the fungus mycelia on the surface of the fungus mycelia; the formation of mycelium pellets can be accelerated, the specific surface area of the filler structure is large, the hollow mycelium pellets formed by attaching fungus mycelia to the surface of the filler structure have good stability, the structure is uniform and compact and is not easy to loosen, compared with the common solid mycelium pellets, the filler structure is more conducive to oxygen transmission and diffusion, and can be effectively used in the fields of sewage treatment and biological fermentation. The invention has good compression resilience rate, the compression resilience rate is up to 94-99%, and the spring back can be fast realized after the spring back is extruded, so the spring back is free from deformation and damage, has long service life and can be recycled.
Which has the following components.
1) When shaking the shaking table or the fermentation cylinder is cultivated, the conical thorn structure is easy to increase the shearing force, can be beneficial to the fungus mycelium to wind on the surface of the conical thorn structure, and the filler provides more mycelium proliferation space, thereby being beneficial to the fungus mycelium to grow in an attached way outside the net.
2) The diameter of the inscribed sphere of the regular dodecahedron shell of the filler is 5-10 mm (preferably 5 mm), and the effective specific surface area of the biological filler is large, so that the comprehensive performance of the biological filler can be improved to a greater extent.
3) The packing material has good compression resilience rate which is up to 94% -99%, can rebound rapidly after being extruded, cannot deform and be damaged, can reduce abrasion between a tank body and equipment, and prolongs the service life of the whole system.
4) The filler is improved, the material is HDPE material, and the density is 0.941-0.960 g/cm 3 The density of the composite mycelium pellets formed with the filler is about 1.004-1.050 g/cm 3 Near the water, the water is in a suspension state, so that the water can be well fluidized by using lower energy consumption.
5) The composite mycelium pellet formed by the invention has good structural stability, and is more beneficial to separation of mycelium pellets and water during sewage treatment application.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention.
Fig. 2 is a schematic structural view of a regular dodecahedron-shaped shell according to the present invention.
FIG. 3 is a schematic view of the junction of the conical spike and the regular dodecahedron-shaped shell of the present invention.
The specific embodiment is as follows: the following describes the embodiments of the present invention in detail with reference to the drawings.
As shown in figures 1-3, the filler formed by the auxiliary mycelium pellet for sewage treatment is made of high-density polyethylene (HDPE) with the density of 0.941-0.960 g/cm 3 . The overall shape is a regular dodecahedron shape, which comprises a regular dodecahedron shape shell 1 and a conical thorn structure 2 on the outer surface of the shell, wherein the diameter of an inscribed sphere of the regular dodecahedron shape shell is 5-10 mm (preferably 5 mm), the regular dodecahedron shape shell 1 is a framework formed by round edge lines 1-1, and the diameters of the round edge lines 1-1 are 0.2 mm; the conical thorn structure 2 is composed of a plurality of conical thorns 2-1, the conical thorns 2-1 are perpendicular to the regular pentagon single faces of the regular dodecahedron-shaped shell, 30 conical thorns 2-1 are uniformly distributed on the circular ridge line of the regular pentagon of each single face, the height of the conical thorns 2-1 is 3 mm, and the bottom surface diameter is 0.2 mm.
The preparation method of the filler comprises the following steps: the material is prepared by adopting an integral injection molding mode, and after production, the material is sampled and subjected to compression rebound rate test by referring to a test method in JB/T6371-2008 of carbon fiber woven filler.
The specific experimental steps are as follows: and a testing machine with the precision of 0.01KN is adopted, and 10 fillers are taken as a group of samples. And (3) uniformly applying the initial load to 0.5 MPa, uniformly loading to 2.5 MPa within 10s, recording the deformation under the final load after maintaining for 60s, immediately unloading to the initial load, and recording the deformation after maintaining for 60 s.
The compression rebound rate test was performed by sampling three times according to the above test procedure, and the test results were as follows.
Sample group: the average rebound rate was 97.5%.
Sample two groups: the average rebound rate is 98.1%.
Sample three groups: the average rebound rate was 97.3%.
The three groups of the carrier fillers have the average rebound rate of 97.6 percent, so the carrier fillers have high compression rebound rate, are not easy to deform and damage, can be repeatedly used and have long service life.
It should be understood that parts of the present specification not specifically described are prior art. The above examples are merely illustrative of preferred embodiments of the invention, and other preferred embodiments are not explicitly described herein and do not limit the scope of the invention.
Claims (3)
1. A filler for assisting fungus mycelium pellet formation is characterized by comprising a regular dodecahedron-shaped shell (1) and a conical thorn structure (2) on the outer surface of the shell, wherein the conical thorn structure (2) consists of a plurality of conical thorns (2-1), each conical thorn (2-1) is perpendicular to one regular pentagon surface of the regular dodecahedron-shaped shell, the filler is made of high-density polyethylene, and the density is 0.941-0.960 g/cm 3 。
2. The filler for assisting the formation of mycelium pellets of fungi according to claim 1, wherein the inscribed circle diameter of the regular dodecahedron-shaped shell is 5 mm, and the diameters of the circular ridges (1-1) are 0.2 mm; the height of the conical thorn (2-1) is 3 mm, and the diameter of the bottom surface of the conical thorn (2-1) is 0.2 mm.
3. A filler for assisting the formation of mycelium pellets according to claim 1, characterized in that 30 conical spines (2-1) are evenly distributed on the five rounded edges (1-1) of the regular pentagonal single face.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911144982.0A CN111039393B (en) | 2019-11-21 | 2019-11-21 | Filler for assisting formation of fungus mycelium pellets |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911144982.0A CN111039393B (en) | 2019-11-21 | 2019-11-21 | Filler for assisting formation of fungus mycelium pellets |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111039393A CN111039393A (en) | 2020-04-21 |
| CN111039393B true CN111039393B (en) | 2024-01-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911144982.0A Active CN111039393B (en) | 2019-11-21 | 2019-11-21 | Filler for assisting formation of fungus mycelium pellets |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111039393B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024062136A1 (en) * | 2022-09-23 | 2024-03-28 | Mycrobez Ag | Means and methods for the preparation of a mycelium-colonized substrate |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4332903A (en) * | 1980-08-01 | 1982-06-01 | Purdue Research Foundation | Media manipulation for preparing biologically active hollow mycelial pellets |
| CN1978638A (en) * | 2005-11-30 | 2007-06-13 | 中国科学院沈阳应用生态研究所 | Immohilized microorganism carrier and its use |
| CN201254486Y (en) * | 2008-08-04 | 2009-06-10 | 潘坚强 | Suspension filler for effluent purification |
| CN104004743A (en) * | 2014-05-15 | 2014-08-27 | 昆明理工大学 | Application of vascular carrier in immobilization culture of filamentous microorganisms |
| CN104045149A (en) * | 2014-07-07 | 2014-09-17 | 中国科学院重庆绿色智能技术研究院 | Biological stuffing for wastewater treatment |
| CN105198096A (en) * | 2015-11-10 | 2015-12-30 | 重庆融极环保工程有限公司 | Device for lowering assimilable organic carbon in circulating water |
| CN105293720A (en) * | 2015-11-10 | 2016-02-03 | 重庆融极环保工程有限公司 | Method for reducing assimilable organic carbon in circulating water through biomembrane method |
| CN206089167U (en) * | 2016-08-26 | 2017-04-12 | 新疆浙源环境工程有限公司 | Immobilized microorganism aerated filter adsorption system |
| CN110157603A (en) * | 2019-06-20 | 2019-08-23 | 南京高新工大生物技术研究院有限公司 | A kind of Immobilized Aspergillus niger produces the fermentation reactor and its method of citric acid |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2223600A1 (en) * | 2009-02-19 | 2010-09-01 | Urea Casale S.A. | Granules containing filamentary fungi and method of preparation thereof |
-
2019
- 2019-11-21 CN CN201911144982.0A patent/CN111039393B/en active Active
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|---|---|---|---|---|
| US4332903A (en) * | 1980-08-01 | 1982-06-01 | Purdue Research Foundation | Media manipulation for preparing biologically active hollow mycelial pellets |
| CN1978638A (en) * | 2005-11-30 | 2007-06-13 | 中国科学院沈阳应用生态研究所 | Immohilized microorganism carrier and its use |
| CN201254486Y (en) * | 2008-08-04 | 2009-06-10 | 潘坚强 | Suspension filler for effluent purification |
| CN104004743A (en) * | 2014-05-15 | 2014-08-27 | 昆明理工大学 | Application of vascular carrier in immobilization culture of filamentous microorganisms |
| CN104045149A (en) * | 2014-07-07 | 2014-09-17 | 中国科学院重庆绿色智能技术研究院 | Biological stuffing for wastewater treatment |
| CN105198096A (en) * | 2015-11-10 | 2015-12-30 | 重庆融极环保工程有限公司 | Device for lowering assimilable organic carbon in circulating water |
| CN105293720A (en) * | 2015-11-10 | 2016-02-03 | 重庆融极环保工程有限公司 | Method for reducing assimilable organic carbon in circulating water through biomembrane method |
| CN206089167U (en) * | 2016-08-26 | 2017-04-12 | 新疆浙源环境工程有限公司 | Immobilized microorganism aerated filter adsorption system |
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