CN111039390B - Preparation method of microbial carbon source - Google Patents
Preparation method of microbial carbon source Download PDFInfo
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- CN111039390B CN111039390B CN201911370491.8A CN201911370491A CN111039390B CN 111039390 B CN111039390 B CN 111039390B CN 201911370491 A CN201911370491 A CN 201911370491A CN 111039390 B CN111039390 B CN 111039390B
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 85
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 85
- 230000000813 microbial effect Effects 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 59
- 238000003756 stirring Methods 0.000 claims abstract description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 244000005700 microbiome Species 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 8
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims abstract description 8
- 239000008103 glucose Substances 0.000 claims abstract description 8
- 239000001632 sodium acetate Substances 0.000 claims abstract description 8
- 235000017281 sodium acetate Nutrition 0.000 claims abstract description 8
- 238000005303 weighing Methods 0.000 claims abstract description 8
- 150000001298 alcohols Chemical class 0.000 claims abstract description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 5
- 238000002791 soaking Methods 0.000 claims abstract description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 39
- 239000007788 liquid Substances 0.000 claims description 34
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 24
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 claims description 20
- 238000013268 sustained release Methods 0.000 claims description 16
- 239000012730 sustained-release form Substances 0.000 claims description 16
- 229960002337 magnesium chloride Drugs 0.000 claims description 12
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 12
- 235000011056 potassium acetate Nutrition 0.000 claims description 10
- GDVKFRBCXAPAQJ-UHFFFAOYSA-A dialuminum;hexamagnesium;carbonate;hexadecahydroxide Chemical compound [OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Al+3].[Al+3].[O-]C([O-])=O GDVKFRBCXAPAQJ-UHFFFAOYSA-A 0.000 claims description 9
- 229960001545 hydrotalcite Drugs 0.000 claims description 9
- 229910001701 hydrotalcite Inorganic materials 0.000 claims description 9
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 8
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 8
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 8
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 230000005298 paramagnetic effect Effects 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 229910000859 α-Fe Inorganic materials 0.000 claims description 7
- JGDITNMASUZKPW-UHFFFAOYSA-K aluminium trichloride hexahydrate Chemical compound O.O.O.O.O.O.Cl[Al](Cl)Cl JGDITNMASUZKPW-UHFFFAOYSA-K 0.000 claims description 5
- 229940009861 aluminum chloride hexahydrate Drugs 0.000 claims description 5
- 229940050906 magnesium chloride hexahydrate Drugs 0.000 claims description 5
- DHRRIBDTHFBPNG-UHFFFAOYSA-L magnesium dichloride hexahydrate Chemical compound O.O.O.O.O.O.[Mg+2].[Cl-].[Cl-] DHRRIBDTHFBPNG-UHFFFAOYSA-L 0.000 claims description 5
- 150000001413 amino acids Chemical class 0.000 claims description 4
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 claims description 4
- 239000000292 calcium oxide Substances 0.000 claims description 4
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 4
- 229960004109 potassium acetate Drugs 0.000 claims description 4
- 229940088594 vitamin Drugs 0.000 claims description 4
- 229930003231 vitamin Natural products 0.000 claims description 4
- 235000013343 vitamin Nutrition 0.000 claims description 4
- 239000011782 vitamin Substances 0.000 claims description 4
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 4
- 230000032683 aging Effects 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 238000000967 suction filtration Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 claims description 2
- 238000012805 post-processing Methods 0.000 claims description 2
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 claims 2
- 229940063656 aluminum chloride Drugs 0.000 claims 1
- 239000010865 sewage Substances 0.000 abstract description 4
- 239000002351 wastewater Substances 0.000 description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 8
- 239000011574 phosphorus Substances 0.000 description 8
- 229910052698 phosphorus Inorganic materials 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 239000010802 sludge Substances 0.000 description 7
- 238000004065 wastewater treatment Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 150000003384 small molecules Chemical class 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000009830 intercalation Methods 0.000 description 2
- 230000002687 intercalation Effects 0.000 description 2
- 230000003446 memory effect Effects 0.000 description 2
- SNAAJJQQZSMGQD-UHFFFAOYSA-N aluminum magnesium Chemical compound [Mg].[Al] SNAAJJQQZSMGQD-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000005291 magnetic effect Effects 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229940083608 sodium hydroxide Drugs 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Water Treatment By Sorption (AREA)
Abstract
The invention discloses a preparation method of a microbial carbon source, which relates to the field of sewage treatment and comprises the following steps: s1: weighing raw materials, namely weighing the following raw materials for preparing the microbial carbon source in parts by weight: 10-20 parts of glucose, 20-40 parts of alcohol, 20-40 parts of sodium acetate and 200 parts of water; s2: mixing the raw materials, namely adding glucose, alcohols and sodium acetate into water, and uniformly mixing and stirring to obtain a stirring solution; s3: adsorbing the slow release material, namely soaking the slow release material in the stirring solution for 10-50 minutes, taking out and drying to obtain a slow release material carbon source; the carbon source of the microorganism can be obtained through the 3 steps. The preparation method has the advantage of improving the durability of the microbial carbon source.
Description
Technical Field
The invention relates to the technical field of wastewater treatment, in particular to a preparation method of a microbial carbon source.
Background
At present, the denitrification and dephosphorization of wastewater by utilizing the action of microorganisms is widely applied.
In the prior art, nitrifying bacteria and denitrifying bacteria are mainly used for decomposing and removing nitrogen-containing compounds in wastewater, and dephosphorizing bacteria are used for decomposing and removing phosphorus-containing pollutants in wastewater. The soluble carbon source capable of being utilized in the wastewater has very obvious influence on the nitrogen and phosphorus removal effect of microorganisms in a water body, and when the COD concentration of the inlet water of the wastewater is lower, the carbon source is difficult to meet the growth requirements of denitrifying bacteria and phosphorus removing bacteria, so that the nitrogen and phosphorus removal efficiency is lower. Especially in sewage in some areas in south China, the concentration of organic matters is low, and the basic requirements of nitrogen and phosphorus removal cannot be met generally. So it is usually necessary to add some supplementary carbon source which is easily degradable.
Liquid carbon sources such as methanol and ethanol are often used as a carbon source for supplementing in general, but when the liquid carbon source is added alone, the efficiency is reduced because the liquid carbon source needs to be frequently supplemented because of the advantages of easy reduction and high reaction speed.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a preparation method of a microbial carbon source, which is characterized in that a slow-release carbon source material is obtained by compounding a small-molecular carbon source and a slow-release material, so that the sustainability of the carbon source is improved.
The above object of the present invention is achieved by the following technical solutions:
a preparation method of a microbial carbon source comprises the following steps:
s1: weighing raw materials, namely weighing the following raw materials for preparing the microbial carbon source in parts by weight:
s2: mixing the raw materials, namely adding glucose, alcohols and sodium acetate into water, and uniformly mixing and stirring to obtain a stirring solution;
s3: adsorbing the slow release material, namely soaking the slow release material in the stirring solution for 10-50 minutes, taking out and drying to obtain a slow release material carbon source;
the carbon source of the microorganism can be obtained through the 3 steps.
By adopting the technical scheme, the glucose, the alcohols and the sodium acetate are all micromolecular carbon sources, and the carbon source in the wastewater can be supplemented when the COD concentration of inlet water is lower during the biological treatment of the wastewater, so that enough carbon source is contained in the wastewater to meet the growth requirements of denitrifying bacteria and phosphorus removing bacteria commonly used in the biological method, and the nitrogen and phosphorus removal efficiency of microorganisms is improved. In the preparation process of the microbial carbon source, the raw material micromolecule carbon source is mixed and then adsorbed by the slow release material, so that when the micromolecule carbon source is added into the wastewater, the slow release material has a controlled release effect on the adsorbed micromolecule carbon source, the amount of the micromolecule carbon source released into the wastewater can be better controlled, and the phenomenon that the COD value in the wastewater is increased due to the overlarge addition amount of the carbon source is avoided.
The invention is further configured to: the sustained-release material is prepared from the following raw materials in parts by weight:
by adopting the technical scheme, magnesium chloride hexahydrate, aluminum chloride hexahydrate, potassium acetate, sodium hydroxide and water can be used for preparing the magnesium-aluminum hydrotalcite material with acetate ion intercalation, and the hydrotalcite has a layered double hydroxide with a micropore structure and a memory effect. The carbon source micro-molecule is adsorbed due to the large specific surface area, and the layered structure can be recovered by roasting after the carbon source micro-molecule is used and recovered due to the memory effect, so that the carbon source micro-molecule can be recovered and utilized. Meanwhile, the polarity of the hydrotalcite is enhanced through the intercalation of acetate ions, and when the hydrotalcite is used for adsorbing a small-molecule carbon source, the small-molecule carbon source is a polar substance, so that the small-molecule carbon source can be better adsorbed by the hydrotalcite, and the adsorption efficiency and the adsorption capacity of the hydrotalcite are improved.
The invention is further configured to: the preparation process of the sustained-release material specifically comprises the following steps:
step a: mixing and dissolving magnesium chloride hexahydrate, aluminum chloride hexahydrate, potassium acetate and water according to the weight ratio of 1: 5 respectively; (ii) a
Step b: mixing sodium hydroxide and water according to a proportion to obtain a sodium hydroxide solution;
step c: b, adding the magnesium chloride solution and the potassium acetate solution obtained in the step a into a sodium hydroxide solution, adjusting the pH to 9-10, and then adding the magnesium chloride solution to obtain a solid-liquid mixture;
step d: and aging the solid-liquid mixture at 65-70 ℃ for 20-24 h, then carrying out suction filtration and washing, and finally drying to obtain the intercalated hydrotalcite.
The invention is further configured to: before the slow release material is added into the stirring liquid, ethanol steam is sprayed on the slow release material for 3-6 min, and then the slow release material is added into the stirring liquid.
By adopting the technical scheme, when the slow release material is added into the stirring liquid, the stirring liquid can easily form a layer of liquid film on the surface of the slow release material, and the liquid film can seal micropores in the slow release material, so that air in part of the micropore structure of the slow release material is sealed to prevent the slow release material from further absorbing the micromolecular carbon source. After the ethanol steam is sprayed on the slow release material, more ethanol steam can be filled in the microporous structure of the slow release material, and when the slow release material is added into the stirring liquid, the ethanol steam in the microporous structure of the slow release material is cooled and shrunk due to the cooling of the slow release material by the stirring liquid, so that a certain negative pressure is generated, and both the micromolecular carbon source and the solvent in the stirring liquid can partially enter the micropores of the slow release material. And after the part of the stirring liquid enters the micropores, ethanol steam in the microporous structure can be dissolved in the stirring liquid, so that negative pressure in the micropores is always generated until the stirring liquid completely permeates in the micropores, and the micromolecular carbon source in the stirring liquid is adsorbed to the surface of the micropores in the permeating process of the stirring liquid, so that the adsorption effect of the slow release material on the micromolecular carbon source is improved.
The invention is further configured to: and c, adding the magnesium chloride solution and the potassium acetate solution into the sodium hydroxide solution and adding paramagnetic ferrite powder at the same time, wherein the mass ratio of the paramagnetic ferrite powder to the magnesium chloride is 1: 1-2, and after the magnesium chloride solution is added, carrying out ultrasonic-assisted reaction.
By adopting the technical scheme, the prepared slow release material has magnetism after the paramagnetic ferrite powder is added, so that the slow release material after adsorbing the small molecular carbon source can be conveniently recycled after being added into wastewater for treatment, and can be rapidly collected under the action of an external magnetic field, thereby avoiding secondary pollution of the slow release material to the wastewater, simultaneously improving the recycling efficiency of resources and realizing sustainable operation of the process.
The invention is further configured to: step S4 follows step S3: post-processing, the step S4 operates as follows: carrying out post-treatment on the carbon source adsorbed by the sustained-release material obtained in the step S3 by using a post-treatment solution, drying and crushing the carbon source adsorbed by the sustained-release material after the post-treatment is finished, and sieving the crushed carbon source by using a sieve of 10-50 meshes to obtain a microbial carbon source;
the post-treatment liquid comprises the following components in parts by weight:
1-3 parts of nano calcium oxide;
80-100 parts of carboxymethyl cellulose;
200-300 parts of cyclohexane.
By adopting the technical scheme, a carboxymethyl cellulose coating is attached to the surface of the slow release material adsorption carbon source after the post-treatment process, and nano calcium oxide is dispersed in the carboxymethyl cellulose coating. When the microbial carbon source obtained after the post-treatment is added into the wastewater, the carboxymethyl cellulose coating on the surface of the microbial carbon source begins to dissolve when meeting water, and a small amount of heat is released after the nano calcium oxide in the carboxymethyl cellulose coating is contacted with the water in a dispersing manner in the dissolving process, so that the temperature of the microbial carbon source is increased, and therefore the micromolecule carbon source in the sustained-release material in the microbial carbon source is activated, the micromolecule carbon source is more easily released from the sustained-release material, the reaction of the sustained-release material is more sensitive, and the carbon source is more timely released.
The invention is further configured to: the post-treatment liquid is also added with 0.5-1 part of amino acid and 0.5-1 part of vitamin according to the parts by weight.
By adopting the technical scheme, in the process of dissolving the carboxymethyl cellulose in the wastewater, the amino acid and the vitamin can be released into the wastewater from the surface of the carbon source of the microorganism along with the dissolution of the carboxymethyl cellulose, and the amino acid and the vitamin can supplement the nutrition required by the growth and the reproduction of the microorganism, so that the effect of the microorganism is improved, and the treatment of the microorganism on nitrogen and phosphorus in the wastewater is improved.
Compared with the prior art, the invention has the beneficial effects that:
1. the slow-release material and the micromolecular carbon source are compounded to obtain the microbial carbon source with the slow-release effect, so that the durability of the micromolecular carbon source in wastewater treatment is improved;
2. by adding paramagnetic iron oxide powder, the slow-release material can be conveniently and quickly collected and recycled after the wastewater treatment process is finished.
Detailed Description
The present invention will be described in detail with reference to examples.
The invention discloses a preparation method of a microbial carbon source, which is characterized by comprising the following steps:
s1: weighing raw materials, namely weighing the following raw materials for preparing the microbial carbon source in parts by weight:
wherein the alcohol is ethanol;
s2: mixing the raw materials, namely adding glucose, alcohols and sodium acetate into water, and uniformly mixing and stirring to obtain a stirring solution;
s3: adsorbing by using a slow release material, spraying ethanol steam on the slow release material for treating for 3min, then soaking the slow release material in the stirring solution for 50 min, taking out and drying to obtain a slow release material carbon source;
s4: post-treatment, namely post-treating the carbon source adsorbed by the sustained-release material obtained in the step S3 by using post-treatment liquid, drying and crushing the carbon source adsorbed by the sustained-release material after the post-treatment is finished, and sieving the dried and crushed carbon source with a 50-mesh sieve to obtain a microbial carbon source;
the post-treatment liquid comprises the following components in parts by weight:
the carbon source of the microorganism can be obtained through the 3 steps.
The sustained-release material is prepared from the following raw materials in parts by weight:
the preparation process of the release material specifically comprises the following steps:
step a: respectively mixing and dissolving magnesium chloride hexahydrate, aluminum chloride hexahydrate, potassium acetate and water according to the weight ratio of 1: 5; (ii) a
Step b: mixing sodium hydroxide and water according to a proportion to obtain a sodium hydroxide solution;
step c: b, adding the magnesium chloride solution and the potassium acetate solution obtained in the step a into a sodium hydroxide solution, adding paramagnetic ferrite powder, wherein the mass ratio of the paramagnetic ferrite powder to the magnesium chloride is 1: 2, then adjusting the pH value to 9, then adding the magnesium chloride solution, and carrying out ultrasonic-assisted reaction to obtain a solid-liquid mixture;
step d: and aging the solid-liquid mixture at 70 ℃ for 24h, then carrying out suction filtration and washing, and finally drying to obtain the intercalated hydrotalcite.
The difference between the examples 2-5 and the example 1 is that the raw materials for preparing the microbial carbon source comprise the following components in parts by weight.
The difference between the examples 6-9 and the example 1 lies in that the components in the raw materials for preparing the sustained-release material are listed in the following table according to parts by weight.
Examples 10 to 13 are different from example 1 in that the following components are listed in the following table in parts by weight in the post-treatment liquid.
Examples 14 to 17 are different from example 1 in that the treatment time of the sustained-release material with ethanol vapor sprayed thereon is shown in the following table.
| Examples | Example 14 | Example 15 | Example 16 | Example 17 |
| Time (min) | 3.75 | 4.5 | 5.25 | 6 |
Examples 18 to 21 are different from example 1 in that the immersion time of the stirring liquid in step S3 is shown in the following table.
| Examples | Example 18 | Example 19 | Example 20 | Example 21 |
| Time (min) | 20 | 30 | 40 | 50 |
Comparative example
Comparative example 1 differs from example 1 in that the microbial carbon source employs a mixture of glucose, ethanol and sodium acetate;
comparative example 2 differs from example 1 in that the microbial carbon source was not post-treated with a post-treatment solution.
Detection method
30L of sewage was taken from the same batch of sewage to be treated, and equally divided into 3 samples, which were designated as sample A, sample B, and sample C, respectively. Samples A, B, C were each treated using an upflow anaerobic sludge blanket reactor.
Adding the microbial carbon source in the example 1 into the upflow anaerobic sludge blanket reactor when the sample A is treated; adding the microbial carbon source in the comparative example 1 into the upflow anaerobic sludge blanket reactor when treating the sample B; adding the microbial carbon source in the comparative example 2 into the upflow anaerobic sludge blanket reactor when treating the sample C; the amounts of the carbon sources added to the microorganisms in example 1, comparative example 1 and comparative example 2 were the same and were all 100 g.
The samples A, B and C pass through the upflow anaerobic sludge blanket reactor at a flow rate of 1L/h, and if the COD value is not lower than 100 after the passing, the wastewater treated by the upflow anaerobic sludge blanket reactor circularly flows into the upflow anaerobic sludge blanket reactor until the COD value of the effluent wastewater is reduced to be lower than 100mg/L, and the treatment amounts of the samples A, B and C are recorded, and the results are shown in the following table.
| Group of | Wastewater treatment capacity (L) |
| Sample A | 7.5 |
| Sample B | 4.2 |
| Sample C | 5.4 |
And (4) conclusion: through the tests in the table above, the total amount of wastewater treatment is more when the sample A is used for treating wastewater, and the total amount of wastewater treatment of the sample B is the least. The total wastewater treatment amount of sample B is between that of sample A and sample C, which shows that the post-treatment of the post-treatment liquid has a certain influence on the carbon source of the microorganism in the present application.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.
Claims (3)
1. A preparation method of a microbial carbon source is characterized by comprising the following steps:
s1: weighing raw materials, namely weighing the following raw materials for preparing the microbial carbon source in parts by weight:
10-20 parts of glucose;
20-40 parts of alcohols;
20-40 parts of sodium acetate;
water 160 and 200 portions;
s2: mixing the raw materials, namely adding glucose, alcohols and sodium acetate into water, and uniformly mixing and stirring to obtain a stirring solution;
s3: adsorbing the slow release material, namely soaking the slow release material in the stirring solution for 10-50 minutes, taking out and drying to obtain a slow release material carbon source;
step S4 follows step S3: post-processing, the step S4 operates as follows: carrying out post-treatment on the carbon source adsorbed by the sustained-release material obtained in the step S3 by using a post-treatment solution, drying and crushing the carbon source adsorbed by the sustained-release material after the post-treatment is finished, and sieving the crushed carbon source by using a sieve of 10-50 meshes to obtain a microbial carbon source;
the post-treatment liquid comprises the following components in parts by weight:
1-3 parts of nano calcium oxide;
80-100 parts of carboxymethyl cellulose;
200-300 parts of cyclohexane;
the microbial carbon source can be obtained through the steps;
the sustained-release material is prepared from the following raw materials in parts by weight:
30-60 parts of magnesium chloride hexahydrate;
10-20 parts of aluminum chloride hexahydrate;
15-30 parts of potassium acetate;
5-15 parts of sodium hydroxide;
160-200 parts of water;
the preparation process of the sustained-release material specifically comprises the following steps:
step a: mixing and dissolving magnesium chloride hexahydrate, aluminum chloride hexahydrate, potassium acetate and water according to the weight ratio of 1: 5 respectively;
step b: mixing sodium hydroxide and water in proportion to obtain a sodium hydroxide solution;
step c: b, adding the magnesium chloride solution and the potassium acetate solution obtained in the step a into a sodium hydroxide solution, adjusting the pH to 9-10, and then adding the magnesium chloride solution to obtain a solid-liquid mixture;
step d: aging the solid-liquid mixture at 65-70 ℃ for 20-24 h, then carrying out suction filtration and washing, and finally drying to obtain intercalated hydrotalcite;
before the slow release material is added into the stirring liquid, ethanol steam is sprayed on the slow release material for 3-6 min, and then the slow release material is added into the stirring liquid.
2. The method for preparing a carbon source for microorganisms according to claim 1, wherein: and c, adding the magnesium chloride solution and the potassium acetate solution into the sodium hydroxide solution and adding paramagnetic ferrite powder at the same time, wherein the mass ratio of the paramagnetic ferrite powder to the magnesium chloride is 1: 1-2, and after the aluminum chloride solution is added, carrying out ultrasonic-assisted reaction.
3. The method for preparing a carbon source for microorganisms according to claim 1, wherein: the post-treatment liquid is also added with 0.5-1 part of amino acid and 0.5-1 part of vitamin by weight.
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