CN111024661B - Fluorescence detection card and detection method for environmental pollutants - Google Patents

Abstract

The invention provides a fluorescent detection card for environmental pollutants, and relates to the technical field of pollutant detection. The utility model provides an environmental contaminant's fluorescence detection card, includes first fluorescence pick-up plate, first detection window, and first fluorescence pick-up plate articulates there is second fluorescence pick-up plate, and second fluorescence pick-up plate is equipped with the second and detects the groove, has solved current detection card structure simple relatively, leads to fluorescence detection card to use the problem that the limitation is big. A method for detecting environmental pollutants by using a fluorescence detection card comprises the steps of adjusting the overlapping of a first fluorescence detection plate and a second fluorescence detection plate, adding a reagent, adding a detection sample and carrying out photoelectric treatment, and can realize the fluorescence detection of a small number of groups of information of the environmental pollutants. A method for detecting environmental pollutants by using a fluorescence detection card comprises the steps of adjusting a first fluorescence detection plate and a second fluorescence detection plate to be parallel, adding a reagent, adding a detection sample and carrying out photoelectric treatment, and can realize the fluorescence detection of information of a large number of groups of environmental pollutants.

Description

Fluorescence detection card and detection method for environmental pollutants

technical field

The invention relates to the technical field of pollutant detection, in particular, to a fluorescent detection card and a detection method for environmental pollutants.

Background technique

Environmental pollutants refer to substances that change the normal composition and nature of the environment after entering the environment, directly or indirectly harm the survival of human beings or cause the decline of the natural ecological environment, and are the object of environmental monitoring research. Most environmental pollutants are produced by human production and living activities. Some substances are originally useful substances in production, and even essential nutrients for humans and organisms, but they are discharged in large quantities due to underutilization, which not only causes waste of resources, but may also become environmental pollutants.

Environmental pollutants are mainly organic pollutants. At present, the detection methods of organic pollutants (organic compounds such as polycyclic hydrocarbons) in industry are mainly divided into two categories: chemical and biological: the former are mostly chromatography and mass spectrometry, and the equipment is large and expensive. The detection items are single, the throughput is small, the sample pretreatment is relatively complicated, and new unknown substances in the sample cannot be detected; some aldehydes are combined with dyes to form colored compounds by visible light spectrophotometry. The sensitivity is low, and the detection items are very limited. Biological detection methods, such as uterine growth experiment, vitellogenin production experiment in hepatocytes, human breast cancer cell (MCF-7) proliferation experiment, recombinant gene yeast experiment, yeast two-hybrid experiment and other five main environmental estrogen biological detection methods , The instrument requires a microplate reader, an animal cell incubator, an ultra-clean bench and a related ultra-clean working environment. The method is cumbersome, time-consuming, costly, and the detection sample is single. These methods are not suitable for grassroots and field rapid detection.

In order to improve the detection quality of environmental organic pollutants in industry, a fluorescence detection method has emerged. Fluorescence detection is a natural luminescence reaction. Through the reaction of luciferase and ATP, it can detect human cells, bacteria, molds, and food residues. Get reaction results within 15 seconds. The illuminance is measured by special equipment and expressed in digital form. In the fluorescence detection, the non-cellular expression system reagents and detection samples are filled on the fluorescence detection card, and the fluorescence detection card is combined with the photoelectric technology detection means (the fluorescence detection card and the photoelectric technology detection means constitute the existing organic pollutant detectors) ), to detect the expressed GFP fluorescence intensity, to judge the concentration of organic matter in the organic matter detection sample, and to evaluate the degree of environmental pollutants. The structure of the existing fluorescence detection card is relatively simple, which leads to the problem that the use of the fluorescence detection card is limited.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a fluorescence detection card for environmental pollutants, which solves the problems of the relatively simple structure of the existing detection card and the large limitations in the use of the fluorescence detection card.

The purpose of the present invention is to provide a detection method for detecting environmental pollutants by using a fluorescent detection card, which can realize the fluorescence detection of a few groups (within three groups) information of environmental pollutants.

The purpose of the present invention is to provide a detection method for detecting environmental pollutants by using a fluorescent detection card, which can realize the fluorescence detection of the information of more groups (within six groups) of environmental pollutants.

Embodiments of the present invention are implemented as follows:

A fluorescence detection card for environmental pollutants includes a first fluorescence detection plate, the first fluorescence detection plate is provided with three first detection slots, the bottom of the first detection slots is provided with a detachable first detection window, and the first fluorescence detection One side of the detection plate is hinged with a second fluorescence detection plate that can overlap with it, and the first fluorescence detection plate and the second fluorescence detection plate have the same shape. The second detection slot, the bottom of the second detection slot is provided with a detachable second detection window, and the first detection window and the second detection window are both made of transparent materials.

In some embodiments of the present invention, the first fluorescence detection plate and the second fluorescence detection plate are both made of plastic, the three first detection slots are coaxially arranged on the first fluorescence detection plate, and the three second detection slots The coaxial line is arranged on the second fluorescence detection plate, and the inner sides of the first detection slot and the second detection slot are made of transparent materials.

In some embodiments of the present invention, the first fluorescence detection plate and the second fluorescence detection plate are strip-shaped, the opposite sides of the first fluorescence detection plate and the second fluorescence detection plate are hinged through hinges, and the first fluorescence detection plate and the second fluorescence detection plate are hinged on the opposite sides through hinges. The side of the fluorescence detection board away from the hinge is connected by a lock.

In some embodiments of the present invention, the side of the first fluorescence detection plate and the second fluorescence detection plate away from the hinge is detachably connected by a lock, and an annular groove is provided on the upper side of the second detection slot. The upper side of the device is provided with a convex block that is butted with the annular groove, and the convex block is made of transparent material.

In some embodiments of the present invention, one side of the first detection window is hinged to the first fluorescence detection plate, the other side of the first detection window is detachably connected to the first fluorescence detection plate, and one side of the second detection window is connected to the first fluorescence detection plate. The second fluorescence detection plate is hinged, and the other side of the second detection window is detachably connected to the second fluorescence detection plate.

In some embodiments of the present invention, a card slot is provided on the side of the first fluorescence detection board facing the first detection window, and a side of the first detection window away from its hinge is provided with a coaxial connection with the card slot. In the spherical limiting slot, a spring in a compressed state is arranged in the clamping slot, and a spherical limiting body which can be butted with the spherical limiting slot is connected to one side of the spring, and the spherical limiting body cannot fall off from the clamping slot.

In some embodiments of the present invention, the side of the second fluorescence detection plate facing the second detection window is provided with a card slot, and the side of the second detection window away from its hinge is provided with a coaxial connection with the card slot. In the spherical limiting slot, a spring in a compressed state is arranged in the clamping slot, and a spherical limiting body which can be butted with the spherical limiting slot is connected to one side of the spring, and the spherical limiting body cannot fall off from the clamping slot.

The first detection window and the second detection window are both in the shape of a threaded column, and the screw parts of the first detection window and the second detection window in the threaded column are in a cylindrical shape, and the first detection window and the second detection window in the threaded column are respectively It is connected with the first detection groove and the second detection groove by means of threads.

A detection method for detecting environmental pollutants using a fluorescent detection card:

Adjustment: adjust the first fluorescence detection plate and the second fluorescence detection plate so that the first fluorescence detection plate and the second fluorescence detection plate overlap, and open the three second detection windows on the second fluorescence detection plate;

Adding reagents: Filling three pairs of overlapping first detection slots and second detection slots with acellular Comamonas testosterone expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase through the opened three second detection windows System reagents;

Add test samples: Add environmental pollutants to the overlapping first and second detection tanks, and the non-cellular system reagent of Comamonas testosteroni expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase at 37 degrees Celsius Incubate for 10 minutes, express 3α-hydroxysteroid dehydrogenase/carbonyl reductase acellular expression system reagent of Comamonas testosteroni to produce green fluorescent protein;

Photoelectric processing: Using the principle of photoelectric technology, the first fluorescence detection plate, the second fluorescence detection plate and the photoelectric technology detection method are organically combined to design an environmental dye detector to detect the fluorescence intensity of the expressed green fluorescent protein to determine the environmental pollutants. To evaluate the concentration of organic pollutants, to evaluate the degree of environmental pollutants, the testosterone trichomes were replaced by the green fluorescent protein reporter gene through transgenic technology of Comamonas testosterone expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase. 3α-Hydroxysteroid dehydrogenase/carbonyl reductase gene in monas In the presence of pollutants, the green fluorescent protein is highly expressed, and various organelles and green fluorescent protein reporter genes are obtained through a large number of enrichment and fragmentation of the bacteria, and the transformed 3α-hydroxysteroid dehydrogenase/carbonyl reductase is prepared. A cell-free system reagent for Comamonas testosteroni.

A detection method for detecting environmental pollutants using a fluorescent detection card:

Adjustment: Adjust the first fluorescence detection plate and the second fluorescence detection plate, so that the second fluorescence detection plate is turned away from the first fluorescence detection plate and arranged side by side with the first fluorescence detection plate, so that the first detection window and the second detection window are respectively connected with the first fluorescence detection plate. The bottoms of the first detection slot and the second detection slot are tightly matched;

Reagent addition: Fill the first detection tank and the second detection tank with the Comamonas testosterone non-complex expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase through the upper openings of the first detection tank and the second detection tank, respectively. Cell system reagents;

Add test samples: add environmental pollutants to the first detection tank and the second detection tank, respectively, and the acellular system reagent of Comamonas testosteroni expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase is heated at 37 degrees Celsius. After incubation for 10 minutes, the cell-free expression system reagent of Comamonas testosteroni expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase produces green fluorescent protein;

Photoelectric processing: Using the principle of photoelectric technology, the first fluorescence detection plate, the second fluorescence detection plate and the photoelectric technology detection method are organically combined to design an environmental dye detector to detect the fluorescence intensity of the expressed green fluorescent protein to determine the environmental pollutants. To evaluate the concentration of organic pollutants, to evaluate the degree of environmental pollutants, the testosterone trichomes were replaced by the green fluorescent protein reporter gene through transgenic technology of Comamonas testosterone expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase. 3α-Hydroxysteroid dehydrogenase/carbonyl reductase gene in monas In the presence of pollutants, the green fluorescent protein is highly expressed, and various organelles and green fluorescent protein reporter genes are obtained through a large number of enrichment and fragmentation of the bacteria, and the transformed 3α-hydroxysteroid dehydrogenase/carbonyl reductase is prepared. A cell-free system reagent for Comamonas testosteroni.

The embodiments of the present invention have at least the following advantages or beneficial effects:

The invention provides a fluorescence detection card for environmental pollutants, comprising a first fluorescence detection plate, the first fluorescence detection plate is provided with three first detection grooves, the bottom of the first detection grooves is a detachable first detection window, One side of a fluorescence detection plate is hinged with a second fluorescence detection plate that can overlap with it, and the first fluorescence detection plate and the second fluorescence detection plate have the same shape, and one side of the second fluorescence detection plate is provided with a first detection groove The second detection slot is butted, the bottom of the second detection slot is a detachable second detection window, and both the first detection window and the second detection window are made of transparent materials. In the detection of environmental pollutants with less comparison data, when the second fluorescence detection plate is turned over to the upper side of the first fluorescence detection plate, the first detection slot is connected to the second detection slot, and the second detection window is disassembled, so that the first detection slot is connected to the second detection slot. The first detection slot and the second detection slot are in an open state. When the first detection slot and the second detection slot overlap longitudinally, there are three sets of detection slots (the first detection slot and the second detection slot are integrated), which reduces the occupation of the fluorescence detection card. At the same time, the three sets of detection slots (the first detection slot and the second detection slot are integrated) have a large depth, which can be filled with a large number of reagents and detection samples for detection. When detecting environmental pollutants with more comparison data, the second fluorescence detection plate is turned away from the top of the first fluorescence detection plate, so that the second fluorescence detection plate and the first fluorescence detection plate are arranged side by side, so that the first detection window and the first fluorescence detection plate are arranged side by side. The two detection windows seal one port of the first detection slot and the second detection slot respectively, and add different amounts of detection samples and reagents into the six detection slots (three first detection slots and three second detection slots) respectively , which can realize the detection of multiple data. The switching of the above two detection methods (the overlapping and separation of the first detection slot and the second detection slot) can realize the use of the same fluorescence detection card in different detection states, increase the use range of the fluorescence detection card, and reduce the use of the fluorescence detection card. At the same time, the detachable connection of the first detection window and the second detection window keeps both ends of the first detection slot and the second detection slot open, which is convenient for cleaning the first detection slot and the second detection slot. .

After the first fluorescence detection plate and the second fluorescence detection plate are overlapped, three sets of detection slots (the first detection slot and the second detection slot are integrated) can appear, and different amounts of detection samples and reagents can be added to the three sets of detection slots. Through photoelectric technology processing, small-scale (within three groups) environmental pollutant samples can be detected.

After the first fluorescence detection plate and the second fluorescence detection plate are separated, the first fluorescence detection plate and the second fluorescence detection plate are separated, and three groups of first detection grooves and three groups of second detection grooves can appear. Different amounts of detection samples and reagents are added to the tank and the three groups of second detection tanks, and then processed by photoelectric technology, which can realize large-scale (within six groups) environmental pollutant sample detection.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the following briefly introduces the accompanying drawings used in the embodiments. It should be understood that the following drawings only show some embodiments of the present invention, and therefore do not It should be regarded as a limitation of the scope, and for those of ordinary skill in the art, other related drawings can also be obtained according to these drawings without any creative effort.

1 is a schematic structural diagram of a fluorescent detection card for environmental pollutants according to an embodiment of the present invention;

Fig. 2 is the bottom view of Fig. 1;

Fig. 3 is the full sectional view of A in Fig. 2;

Fig. 4 is the side view of Fig. 1;

5 is a partial schematic diagram of the first fluorescence detection plate and the second fluorescence detection plate after overlapping according to the embodiment of the present invention;

6 is a schematic structural diagram of a detection method for detecting environmental pollutants by using a fluorescent detection card according to an embodiment of the present invention;

7 is a schematic structural diagram when the first fluorescence detection plate and the second fluorescence detection plate are not connected to the first detection window and the second detection window according to the embodiment of the present invention.

Icons: 1-first fluorescence detection plate, 2-hinge, 3-second fluorescence detection plate, 4-second detection groove, 5-ring groove, 6-protrusion, 7-second detection window, 8-section A detection window, 9-ball limit body, 10-spring, 11-card slot, 12-ball limiter slot, 13-lock buckle, 14-environmental dye detector, 15-first detection slot.

Detailed ways

In order to make the purposes, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments These are some embodiments of the present invention, but not all embodiments. The components of the embodiments of the invention generally described and illustrated in the drawings herein may be arranged and designed in a variety of different configurations.

Thus, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

It should be noted that like numerals and letters refer to like items in the following figures, so once an item is defined in one figure, it does not require further definition and explanation in subsequent figures.

In the description of the embodiments of the present invention, it should be noted that if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", The orientation or positional relationship indicated by "outside" is based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship that is usually placed when the product of the invention is used, which is only for the convenience of describing the present invention and simplifying the description, and It is not indicated or implied that the indicated device or element must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as limiting the invention. Furthermore, the terms "first", "second", "third", etc. are only used to differentiate the description and should not be construed as indicating or implying relative importance.

Furthermore, the appearance of the terms "horizontal", "vertical", "overhanging" etc. does not imply that the component is required to be absolutely horizontal or overhanging, but may be slightly inclined. For example, "horizontal" only means that its direction is more horizontal than "vertical", it does not mean that the structure must be completely horizontal, but can be slightly inclined.

In the description of the embodiments of the present invention, "a plurality of" means at least two.

In the description of the embodiments of the present invention, it should also be noted that, unless otherwise expressly specified and limited, the terms "set", "installed", "connected" and "connected" should be understood in a broad sense. It can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, and it can be internal communication between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

Example 1

Please refer to FIGS. 1 and 2. FIG. 1 is a schematic structural diagram of a fluorescent detection card for environmental pollutants according to an embodiment of the present invention, and FIG. 2 is a bottom view of FIG. 1. This embodiment provides a fluorescent detection card for environmental pollutants. , which solves the problem that the structure of the existing detection card is relatively simple, resulting in a large limitation in the use of the fluorescence detection card.

A fluorescence detection card for environmental pollutants, comprising a first fluorescence detection plate 1, the first fluorescence detection plate 1 is provided with three first detection grooves 15, and the bottom of the first detection grooves 15 is provided with a detachable first detection window 8. One side of the first fluorescence detection plate 1 is hinged with a second fluorescence detection plate 3 that can overlap with it, and the first fluorescence detection plate 1 and the second fluorescence detection plate 3 have the same shape, and one side of the second fluorescence detection plate 3 There is a second detection slot 4 that can overlap with the first detection slot 15. The bottom of the second detection slot 4 is provided with a detachable second detection window 7. The first detection window 8 and the second detection window 7 are made of transparent materials. production.

In the detection of environmental pollutants with less comparison data, when the second fluorescence detection plate 3 is turned over to the upper side of the first fluorescence detection plate 1, the first detection groove 15 is docked with the second detection groove 4, and the second detection groove is disassembled. Window 7, so that the first detection slot 15 and the second detection slot 4 are in an open state. When the first detection slot 15 and the second detection slot 4 overlap vertically, there are three sets of detection slots (the first detection slot 15 and the second detection slot 4). 4 integrated), which reduces the floor space of the fluorescence detection card and makes the fluorescence detection card easy to store. At the same time, the three sets of detection slots (the first detection slot 15 and the second detection slot 4 are integrated) have a large depth and can be filled with a large number of Reagents and test samples for testing. When detecting environmental pollutants with more comparison data, the second fluorescence detection plate 3 is turned away from the top of the first fluorescence detection plate 1, so that the second fluorescence detection plate 3 and the first fluorescence detection plate 1 are arranged side by side, so that the first fluorescence detection plate 3 The detection window 8 and the second detection window 7 seal one port of the first detection slot 15 and the second detection slot 4 respectively. Different amounts of test samples and reagents are added to the test, which can realize the detection of multiple data. The switching of the above two detection methods (the first detection slot 15 and the second detection slot 4 overlap and separate) can realize the use of the same fluorescence detection card in different detection states, increase the use range of the fluorescence detection card, and reduce the fluorescence detection card. At the same time, the detachable connection of the first detection window 8 and the second detection window 7 makes both ends of the first detection slot 15 and the second detection slot 4 open, which is convenient for the first detection slot 15 , The second detection tank 4 is cleaned.

The first fluorescence detection plate 1 and the second fluorescence detection plate 3 are both made of plastic. The three first detection slots 15 are coaxially arranged on the first fluorescence detection plate 1, and the three second detection slots 4 are arranged coaxially. On the second fluorescence detection plate 3 , the inner sides of the first detection groove 15 and the second detection groove 4 are made of transparent materials. The first fluorescence detection plate 1 and the second fluorescence detection plate 3 made of plastic can reduce the overall weight of the present invention and have the advantage of being light. The three first detection slots 15 and the three second detection slots 4 are coaxially arranged , which can increase the aesthetics of the appearance of the present invention. The inner sides of the first detection slot 15 and the second detection slot 4 are made of transparent materials, which can increase the reflection efficiency.

Please refer to FIG. 1 and FIG. 5. FIG. 1 is a schematic structural diagram of a fluorescence detection card for environmental pollutants according to an embodiment of the present invention, and FIG. 5 is an embodiment of the present invention after the first fluorescence detection plate 1 and the second fluorescence detection plate 3 are overlapped. The partial schematic diagram shown in the figure, the first fluorescence detection plate 1 and the second fluorescence detection plate 3 are strip-shaped, and the opposite sides of the first fluorescence detection plate 1 and the second fluorescence detection plate 3 are hinged through the hinge 2, and the first fluorescence detection plate 1 and the second fluorescence detection plate 3 are hinged. The side of the second fluorescence detection board 3 away from the hinge is connected by a lock 13 . The hinge 2 can realize fast hinge connection between the first fluorescence detection plate 1 and the second fluorescence detection plate, and can reduce the gap between the hinge ends of the first fluorescence detection plate 1 and the second fluorescence detection plate 3, and increase the first fluorescence detection plate 1 Due to the tightness between the second fluorescence detection plate 3 and the latch 13, when the first fluorescence detection plate 1 and the second fluorescence detection plate 3 are overlapped, the two are fixedly connected to avoid relative shaking between the two.

Please refer to FIG. 1, FIG. 2, and FIG. 4. FIG. 1 is a schematic structural diagram of a fluorescent detection card for environmental pollutants according to an embodiment of the present invention, FIG. 2 is a bottom view of FIG. 1, FIG. 4 is a side view of FIG. One side of the fluorescence detection board 1 and the second fluorescence detection board 3 away from the hinge is detachably connected by a lock 13 , an annular groove 5 is arranged on the upper side of the second detection slot 4 , and an annular slot 5 is arranged on the upper side of the first detection slot 15 . There are bumps butted against the annular groove 5, and the bumps are made of transparent material. The first detection slot 15 and the second detection slot 4 are connected to the annular slot 5 through soil blocks, which can increase the compactness of the time structure of the first detection slot 15 and the second detection slot 4 when they overlap.

One side of the first detection window 8 is hinged to the first fluorescence detection board 1 , and the other side of the first detection window 8 is detachably connected to the first fluorescence detection board 1 . The first detection window 8 is hinged with the first fluorescence detection board 1 , one side of the second detection window 7 is hinged with the second fluorescence detection board 3 , and the other side of the second detection window 7 is connected with the second fluorescence detection board 3 . Removing the connection prevents the first detection window 8 and the second detection window 7 from falling off the first detection fluorescence detection plate and the second fluorescence detection plate 3 when they are disassembled, resulting in damage to the first detection window 8 and the second detection window 7 .

Please refer to FIGS. 2 and 3. FIG. 2 is a bottom view of FIG. 1, and FIG. 3 is a full cross-sectional view of A in FIG. , the side of the first detection window 8 away from its hinge is provided with a spherical limit slot 12 that can be coaxially butt-jointed with the card slot 11, the card slot 11 is provided with a spring 10 in a compressed state, and one side of the spring 10 is connected with a The spherical limiting body 9 can be butted with the spherical limiting groove 12 , and the spherical limiting body 9 cannot fall off from the clamping groove 11 . The spherical limiting body 9 can be contacted and separated from the spherical limiting groove 12 by compressing the spring 10 , so as to achieve a detachable connection between the first detection window 8 and the first fluorescence detection board 1 .

The side of the second fluorescence detection plate 3 facing the second detection window 7 is provided with a clamping slot 11 , and the side of the second detection window 7 away from its hinge is provided with a spherical limit slot that can be coaxially buttted with the clamping slot 11 . 12. A spring 10 in a compressed state is arranged in the card slot 11. A spherical limiting body 9 is connected to one side of the spring 10, which can be butted with the spherical limiting groove 12, and the spherical limiting body 9 cannot fall off from the card slot 11. The spherical limiting body 9 can be contacted and separated from the spherical limiting groove 12 by compressing the spring 10, so as to achieve a detachable connection between the second detection window 7 and the second fluorescence detection plate 3.

Please refer to FIG. 7 . FIG. 7 is a schematic structural diagram of the first fluorescence detection plate, the second fluorescence detection plate 3 and the first detection window and the second detection window according to the embodiment of the present invention. The first detection window 8 and the second detection window are not connected. The windows 7 are all in the shape of a threaded column, and the screw portions of the first detection window 8 and the second detection window 7 in the shape of a threaded column are in a cylindrical shape, and the first detection window 8 and the second detection window 7 in the threaded column are respectively connected with the first detection window 8 and the second detection window 7 in the threaded column shape. The detection groove 15 and the second detection groove 4 are connected by means of threads. The first detection window 8 and the second detection window 7 are connected with the first detection slot 15 and the second detection slot 4 by means of threads, which have the advantages of quick connection and anti-leakage.

Example 2

Please refer to FIG. 1 and FIG. 6. FIG. 1 is a schematic structural diagram of a fluorescent detection card for environmental pollutants according to an embodiment of the present invention, and FIG. 6 is a structure of a detection method for detecting environmental pollutants by using a fluorescent detection card according to an embodiment of the present invention. Schematic diagram, a detection method using a fluorescent detection card to detect environmental pollutants:

Adjustment: adjust the first fluorescence detection plate 1 and the second fluorescence detection plate 3 so that the first fluorescence detection plate 1 and the second fluorescence detection plate 3 overlap, and open the three second detection windows 7 on the second fluorescence detection plate 3;

Adding reagents: filling the three pairs of overlapping first detection slots 15 and second detection slots 4 with testosterone commensal cells expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase through the opened three second detection windows 7 Bacterial acellular system reagents;

Adding test samples: adding environmental pollutants into the overlapping first detection tank 15 and the second detection tank 4, and the non-cellular system reagent of Comamonas testosterone expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase Incubate at 37 degrees Celsius for 10 minutes to express 3α-hydroxysteroid dehydrogenase/carbonyl reductase cell-free expression system reagent of Comamonas testosteroni to produce green fluorescent protein;

Photoelectric processing: Using the principle of photoelectric technology, the first fluorescence detection plate 1, the second fluorescence detection plate 3 and the photoelectric technology detection means are organically combined to design an environmental dye detector 14 to detect the fluorescence intensity of the expressed green fluorescent protein to judge the environment. Concentration of organic pollutants in pollutants, to evaluate the degree of environmental pollutants, and to replace Comamonas testosteroni expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase with green fluorescent protein reporter gene through transgenic technology 3α-hydroxysteroid dehydrogenase/carbonyl reductase gene in Comamonas testosteroni, constructing a fluorescent mutant strain of Comamonas testosterone with 3α-hydroxysteroid dehydrogenase/carbonyl reductase, the mutant strain In the presence of organic pollutants, green fluorescent protein can be expressed efficiently, and various organelles and green fluorescent protein reporter genes can be obtained through a large number of enrichment and fragmentation of bacteria to make transformed 3α-hydroxysteroid dehydrogenase/carbonyl A cell-free system reagent for Comamonas testosteroni for acyl reductase.

After the first fluorescence detection plate 1 and the second fluorescence detection plate 3 overlap, three sets of detection slots (the first detection slot 15 and the second detection slot 4 are integrated) can appear, and different amounts of detection can be added to the three sets of detection slots. Samples and reagents, and then processed by photoelectric technology, can realize the detection of small-scale (within three groups) environmental pollutant samples.

Example 3

Please refer to FIG. 1 and FIG. 6. FIG. 1 is a schematic structural diagram of a fluorescent detection card for environmental pollutants according to an embodiment of the present invention, and FIG. 6 is a structure of a detection method for detecting environmental pollutants by using a fluorescent detection card according to an embodiment of the present invention. Schematic diagram, a detection method using a fluorescent detection card to detect environmental pollutants:

Adjustment: Adjust the first fluorescence detection plate 1 and the second fluorescence detection plate 3 so that the second fluorescence detection plate 3 is turned away from the first fluorescence detection plate 1 and arranged side by side with the first fluorescence detection plate 1, so that the first detection window 8, The second detection window 7 is sealed with the bottoms of the first detection slot 15 and the second detection slot 4 respectively;

Reagent addition: Fill the first detection tank 15 and the second detection tank 4 with testosterone tufts expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase through the upper openings of the first detection tank 15 and the second detection tank 4, respectively Cell-free system reagents for Monosodium;

Adding test samples: adding environmental pollutants into the first detection tank 15 and the second detection tank 4, respectively, and the non-cellular system reagent 37 of Comamonas testosterone expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase Incubate at degrees Celsius for 10 minutes to express 3α-hydroxysteroid dehydrogenase/carbonyl reductase acellular expression system reagent of Comamonas testosteroni to produce green fluorescent protein;

Photoelectric processing: Using the principle of photoelectric technology, the first fluorescence detection plate 1, the second fluorescence detection plate 3 and the photoelectric technology detection means are organically combined to design an environmental dye detector 14 to detect the fluorescence intensity of the expressed green fluorescent protein to judge the environment. Concentration of organic pollutants in pollutants, to evaluate the degree of environmental pollutants, and to replace Comamonas testosteroni expressing 3α-hydroxysteroid dehydrogenase/carbonyl reductase with green fluorescent protein reporter gene through transgenic technology 3α-hydroxysteroid dehydrogenase/carbonyl reductase gene in Comamonas testosteroni, constructing a fluorescent mutant strain of Comamonas testosterone with 3α-hydroxysteroid dehydrogenase/carbonyl reductase, the mutant strain In the presence of organic pollutants, green fluorescent protein can be expressed efficiently, and various organelles and green fluorescent protein reporter genes can be obtained through a large number of enrichment and fragmentation of bacteria to make transformed 3α-hydroxysteroid dehydrogenase/carbonyl A cell-free system reagent for Comamonas testosteroni for acyl reductase.

After the first fluorescence detection plate 1 and the second fluorescence detection plate 3 are separated, the first fluorescence detection plate 1 and the second fluorescence detection plate 3 are separated, and three groups of first detection grooves 15 and three groups of second detection grooves 4 can appear. The three groups of first detection tanks 15 and the three groups of second detection tanks 4 are correspondingly added with different amounts of detection samples and reagents, and then processed by photoelectric technology to achieve large-scale (within six groups) environmental pollutant sample detection.

To sum up, the present invention provides a fluorescent detection card for environmental pollutants, which at least has the following beneficial effects:

When the fluorescence detection card is used to detect environmental pollutants with less comparison data, when the second fluorescence detection plate 3 is turned over to the upper side of the first fluorescence detection plate 1, the first detection slot 15 is docked with the second detection slot 4 and disassembled. In the second detection window 7, the first detection slot 15 and the second detection slot 4 are in an open state. When the first detection slot 15 and the second detection slot 4 are vertically overlapped, there are three sets of detection slots (the first detection slot 15 and the second detection slot 4). The two detection slots 4 are integrated), which reduces the floor space of the fluorescence detection card, and makes the fluorescence detection card easy to store. A large number of reagents and test samples are tested. When detecting environmental pollutants with more comparison data, turn the second fluorescence detection plate 3 away from the top of the first fluorescence detection plate 1, so that the second fluorescence detection plate 3 and the first fluorescence detection plate 1 are arranged side by side, so that the first fluorescence detection plate 3 is arranged side by side with the first fluorescence detection plate 1. The detection window 8 and the second detection window 7 seal one port of the first detection slot 15 and the second detection slot 4 respectively. Different amounts of test samples and reagents are added to the test, which can realize the detection of multiple data. The switching of the above two detection methods (the first detection slot 15 and the second detection slot 4 overlap and separate) can realize the use of the same fluorescence detection card in different detection states, increase the use range of the fluorescence detection card, and reduce the fluorescence detection card. At the same time, the detachable connection of the first detection window 8 and the second detection window 7 makes both ends of the first detection slot 15 and the second detection slot 4 open, which is convenient for the first detection slot 15 , The second detection tank 4 is cleaned.

After the first fluorescence detection plate 1 and the second fluorescence detection plate 3 overlap, three sets of detection slots (the first detection slot 15 and the second detection slot 4 are integrated) can appear, and different amounts of detection can be added to the three sets of detection slots. Samples and reagents, and then processed by photoelectric technology, can realize the detection of small-scale (within three groups) environmental pollutant samples.

After the first fluorescence detection plate 1 and the second fluorescence detection plate 3 are separated, the first fluorescence detection plate 1 and the second fluorescence detection plate 3 are separated, and three groups of first detection grooves 15 and three groups of second detection grooves 4 can appear. The three groups of first detection tanks 15 and the three groups of second detection tanks 4 are correspondingly added with different amounts of detection samples and reagents, and then processed by photoelectric technology to achieve large-scale (within six groups) environmental pollutant sample detection.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims (8)

1. A detection method for detecting environmental pollutants by adopting a fluorescence detection card, wherein the fluorescence detection card comprises a first fluorescence detection plate, three first detection grooves are arranged on the first fluorescence detection plate, a detachable first detection window is arranged at the bottom of each first detection groove, a second fluorescence detection plate which can be overlapped with the first fluorescence detection plate is hinged to one side of the first fluorescence detection plate, the first fluorescence detection plate and the second fluorescence detection plate are in the same shape, a second detection groove which can be overlapped with the first detection groove is arranged at one side of the second fluorescence detection plate, a detachable second detection window is arranged at the bottom of the second detection groove, and the first detection window and the second detection window are both made of transparent materials, and the method is characterized by comprising the following steps of:

adjusting: adjusting the first fluorescence detection plate and the second fluorescence detection plate to enable the first fluorescence detection plate and the second fluorescence detection plate to be overlapped, and opening three second detection windows on the second fluorescence detection plate;

adding a reagent: filling the three pairs of overlapped first detection grooves and second detection grooves with a testosterone comamonas non-cell system reagent for expressing 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase through three opened second detection windows;

adding a detection sample, namely adding environmental pollutants into the overlapped first detection groove and the second detection groove, incubating the environmental pollutants with a Comamonas testosteroni non-cell system reagent for expressing 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase at 37 ℃ for 10 minutes, and expressing the Comamonas testosteroni non-cell expression system reagent for expressing 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase to generate green fluorescent protein;

photoelectric treatment: by utilizing the principle of a photoelectric technology, the first fluorescence detection plate, the second fluorescence detection plate and a photoelectric technology detection means are organically combined to design an environmental dye detector, the fluorescence intensity of expressed green fluorescent protein is detected to judge the concentration of organic pollutants in the environmental pollutants, the degree of the environmental pollutants is evaluated, the Comamonas testosteroni capable of expressing 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase is replaced by a green fluorescent protein reporter gene through a transgenic technology to construct a Comamonas testosteroni fluorescent variant strain of the 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase, the variant strain can efficiently express the green fluorescent protein in the presence of the organic pollutants, and the strains are increased and crushed, obtaining various organelles in the thallus and green fluorescent protein reporter genes to prepare the transformed Comamonas testosteroni non-cell system reagent of the 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase.

2. The method as claimed in claim 1, wherein the first and second fluorescence detection plates are made of plastic, three first detection grooves are coaxially formed on the first fluorescence detection plate, three second detection grooves are coaxially formed on the second fluorescence detection plate, and inner sides of the first and second detection grooves are made of transparent material.

3. The method as claimed in claim 2, wherein the first fluorescence detection plate and the second fluorescence detection plate are strip-shaped, opposite sides of the first fluorescence detection plate and the second fluorescence detection plate are hinged by a hinge, and one sides of the first fluorescence detection plate and the second fluorescence detection plate, which are far away from the hinge, are connected by a lock catch.

4. The detecting method for detecting environmental pollutants by using a fluorescence detecting card as claimed in claim 3, wherein the sides of the first fluorescence detecting plate and the second fluorescence detecting plate away from the hinge joint are detachably connected by a lock catch, an annular groove is arranged in the upper side of the second detecting groove, a convex block butted with the annular groove is arranged on the upper side of the first detecting groove, and the convex block is made of a transparent material.

5. A method as recited in claim 1, wherein one side of said first detection window is hingedly connected to said first fluorescence detection plate, said other side of said first detection window is removably connected to said first fluorescence detection plate, said one side of said second detection window is hingedly connected to said second fluorescence detection plate, and said other side of said second detection window is removably connected to said second fluorescence detection plate.

6. The detecting method for detecting environmental pollutants by using a fluorescence detecting card as claimed in claim 5, wherein a slot is formed in one side of the first fluorescence detecting plate facing the first detecting window, a spherical limiting groove capable of being in coaxial line butt joint with the slot is formed in one side of the first detecting window away from the hinge joint of the first detecting window, a spring in a compressed state is arranged in the slot, a spherical limiting body capable of being in butt joint with the spherical limiting groove is connected to one side of the spring, and the spherical limiting body cannot fall off from the slot.

7. The detecting method for detecting environmental pollutants by using the fluorescent detecting card as claimed in claim 5, wherein a slot is provided at one side of the second fluorescent detecting board facing the second detecting window, a spherical limiting groove capable of being in coaxial line butt joint with the slot is provided at one side of the second detecting window away from the hinge joint, a spring in a compressed state is provided in the slot, a spherical limiting body capable of being in butt joint with the spherical limiting groove is connected to one side of the spring, and the spherical limiting body cannot fall off from the slot.

8. The method as claimed in claim 5, wherein the first and second windows are in a threaded cylindrical shape, and the screw portions of the first and second windows are in a cylindrical shape, and the threaded cylindrical windows are respectively connected to the first and second detection grooves by threads.

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