CN110999828A - Method for changing fish body color distribution pattern - Google Patents
Method for changing fish body color distribution pattern Download PDFInfo
- Publication number
- CN110999828A CN110999828A CN201911318002.4A CN201911318002A CN110999828A CN 110999828 A CN110999828 A CN 110999828A CN 201911318002 A CN201911318002 A CN 201911318002A CN 110999828 A CN110999828 A CN 110999828A
- Authority
- CN
- China
- Prior art keywords
- fish
- body color
- color distribution
- distribution pattern
- incubation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0604—Whole embryos; Culture medium therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides a method for changing fish body color distribution mode, which comprises the steps of giving geldanamycin analogues to a fish oosperm development stage for incubation, wherein incubation water adopted for incubation contains 5 +/-0.1 nmol/L of geldanamycin analogues, the fish oosperm development stage is a first cell stage of the fish oosperm, and the geldanamycin analogues specifically inhibit heat shock protein HSP90 to realize the purpose; the method can quickly, mildly and simply improve the body color distribution pattern of the fish, can also continue the ability in offspring fish individuals, has wide application value, and reduces the economic and technical cost caused by transgenosis or introduction; compared with the natural individual screening and hybridization speed, the method is easier to implement and has low cost compared with a transgenic method; in addition, the method of the invention can cause the body color distribution of the zebra fish to change differently from the parent body color in the experiment in the zebra fish body, thereby having less safety and ethical problems.
Description
Technical Field
The invention belongs to the technical field of ornamental fish variety improvement, and particularly relates to a method for changing fish body color distribution patterns.
Background
At present, there are few methods for improving skin color diversity of fish. Mainly relying on natural individual screening and crossbreeding, transgenic methods are also under development. These methods change the body color distribution ability of fish by selecting individuals whose body color is different from that of the parent species, fixing the body color characteristics by crossing, or by transferring a body color-related gene. However, these methods have the disadvantages of low efficiency, long time or high requirements for technical equipment, and are difficult to rapidly and widely apply to small producers and unusual fishes.
The Chinese patent with publication number CN 105063088A discloses a method for developing an ecological safety type transgenic fluorescent aquarium fish, which belongs to the fields of genetic engineering and molecular breeding of aquarium fish by a fluorescent protein expression vector, a sexual gland tissue specificity regulation expression vector and application of the vector in cultivating the ecological safety type transgenic fluorescent aquarium fish. It has the defects of low efficiency and long time. The transgenic components exist in the gonads of the fluorescent ornamental fishes, so that offspring of the ornamental fishes can breed and transmit transgenic elements, and therefore, the fluorescent ornamental fishes have certain ecological risk problems.
The Chinese invention patent with publication number CN 103609486A discloses a method for breeding and culturing the young fish of the seven-color immortal fish, which adopts the method for breeding and culturing the young fish of the seven-color immortal fish, and comprises different stages of fry culturing, young fish culturing and artificial bait culturing, and can effectively meet the requirements of the growth of the young fish on water quality and nutrition, improve the survival rate of the young fish, promote the rapid growth of the young fish and increase the economic benefit by feeding biological bait types, feeding times, feeding time and water culture conditions of a parent fish spawning tank and a young fish culturing tank in different stages. The method also has the defects of low efficiency, long time or high requirements on technical equipment and the like, and is difficult to rapidly and widely apply to small producers and unusual fishes.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a method for changing the fish body color distribution pattern.
In order to achieve the above purpose, the solution of the invention is as follows:
a method for changing fish body color distribution pattern comprises incubating geldanamycin analog in fish fertilized egg development stage.
Wherein, the structural formula of the geldanamycin analogue is as follows:
in one embodiment of the invention, the incubation is carried out with an aqueous incubation medium containing 5. + -. 0.1nmol/L of the geldanamycin analogue.
In one embodiment of the invention, the developmental stage of the fish zygote is the first cell stage of the fish zygote.
In one embodiment of the invention, the incubation temperature is 28. + -. 1 ℃ and the incubation time is 72. + -.1 h.
In a specific embodiment of the invention, geldanamycin analogs are inhibited by administration of heat shock protein HSP 90.
In one embodiment of the invention, the hatched fry is bred to sexual maturity and then selfed, the fishes are screened out and then selfed for reproduction, and the offspring fishes with different body color distribution from the parents are further screened out.
In summary, the method for changing the body color of fish according to the present invention comprises: and (3) incubating by giving geldanamycin analogues, feeding until sexual maturity for three months, selfing to generate offspring, and screening the fishes with the body color distribution different from that of the parents.
Actually, geldanamycin analog is an inhibitor for specifically inhibiting HSP90 protein, and specifically inhibits the activity of ATPase required by HSP90 protein by competitively binding with the binding site of N-terminal Adenosine Triphosphate (ATP)/Adenosine Diphosphate (ADP) of HSP90 protein to cause the change of HSP90 protein conformation (namely, cause the open conformation of HSP90 to be stable), so that the complex cannot be formed with effector protein and other small molecular proteins to inhibit the normal molecular chaperone function of the complex, and finally cause the degradation of client protein participating in body color formation to further change the body color distribution pattern of zebra fish.
Wherein, the effector protein refers to a protein which is combined with HSP90 at the downstream of HSP 90. Other small molecule proteins are small molecule proteins that bind to HSP 90. The client protein is a protein which can form a complex with HSP90 and has a certain function, namely after the activity of HSP90 is inhibited, the protein which participates in the body color distribution mode cannot form a complex with HSP90 and loses the proper function to degrade.
Due to the adoption of the scheme, the invention has the beneficial effects that:
firstly, compared with the prior transgenic technology, the technology level is higher, for example, the current relatively popular CRISPR-Cas9 gene editing technology, microinjection technology and the like are needed, and the method for changing the color distribution of the zebra fish body by adding the geldanamycin analogue has the advantages that the cost of reagents, technology, instruments and the like is reduced compared with the transgenic technology; the existing strong mutagen causes the conditions of zebra fish malformation, dysplasia and the like, and the caused effects are wider, and compared with the strong mutagen, the method for changing the body color distribution of the zebra fish by adding the geldanamycin analogue is more easily accepted by the public; therefore, the method of the invention is easier to implement and has low cost. In addition, the method of the invention can cause the body color distribution of the zebra fish to change differently from the parent body color in the experiment in the zebra fish body, thereby having less safety and ethical problems.
Secondly, the method of the invention can generate a plurality of different body color distribution changes in 2-3 generations, and is relatively fast compared with natural individual screening and hybridization; the geldanamycin analogue concentration adopted by the invention has no adverse effects such as deformity on the development of zebra fish embryos, and is mild; the invention does not need special equipment such as transgenic equipment, the concentration of the related reagent is not needed to be used in special biological places, the method can be completed in common breeding places, and the operation is simple, convenient and safe. In a word, the invention can rapidly, mildly and simply improve the body color distribution mode of the fish, can continue the ability in offspring fish individuals, and has wide application value, namely, the skin color condition of the ornamental fish can be improved, the ornamental value and the economic benefit of the ornamental fish can be improved, and the economic and technical cost caused by transgenosis or introduction can be reduced.
Detailed Description
The invention provides a method for changing fish body color distribution pattern.
The present invention will be further described with reference to the following examples.
Example (b):
in the embodiment, zebra fish is used as an experimental material, the HSP90 is inhibited (17-AAG, geldanamycin analogue) for 72h at the development stage of fertilized eggs of the zebra fish, and individuals with different body colors from the parents can appear after the developed zebra fish individuals are subjected to self-crossing. Changes in the distribution of body color can be passed on to the offspring.
Wherein, the structural formula of the geldanamycin analogue is as follows:
specifically, the method for changing the body color distribution pattern of zebra fish in the embodiment includes the following steps:
(1) the geldanamycin analogue stock solution has the concentration of 25 mu mol/L, the solvent is dimethyl sulfoxide (DMSO), and the storage is carried out at the temperature of 20 ℃ below zero. When used, the solution is thawed at room temperature and further diluted with common roe incubation water to a final concentration of 5nmol/L of geldanamycin analog.
(2) 10 pairs of eggs (about 1000) from well-developed fish (i.e. sexually mature zebrafish) were mixed and divided into an experimental group (geldanamycin analog) and a control group (DMSO), each group containing about 500 eggs.
(3) And when the fish eggs are incubated with the incubation water (5ml) of the geldanamycin analogue with the final concentration of 5nmol/L in the first cell stage of the fish egg development till the development period is 72 hours, the circulating water (treatment density: 100 particles/ml) of the normal feeding fish is replaced, and when the fish eggs are incubated with the incubation water (5ml) containing 5nmol/L DMSO solvent till the development period is 72 hours in the first cell stage of the fish egg development, the circulating water (treatment density: 100 particles/ml) of the normal feeding fish is replaced.
(4) The experimental and control groups were incubated at 28 ℃ and the 72h post-treatment was complete.
(5) And after the zebra fish is continuously and normally bred to sexual maturity for three months, selfing to generate offspring, and screening the offspring with body color distribution different from that of the parent.
(6) And selfing the zebra fish with the changed body color distribution to obtain the offspring zebra fish with the capability of still 100% of the zebra fish with special body color distribution characteristics.
(7) Compared with the control group, the proportion of the change of the zebra fish offspring color distribution after the zebra fish embryos of the experimental group are treated by 5nmol/L of geldanamycin analogue for 72 hours accounts for 10 percent, and the zebra fish offspring color distribution after the control group is treated by 5nmol/L of DMSO is not changed.
The reason why the control group was treated with DMSO was: the solvent for the geldanamycin analogues was dimethyl sulfoxide (DMSO), and controls were treated with DMSO to exclude the effect of DMSO solvent on the experiment.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. It will be readily apparent to those skilled in the art that various modifications to these embodiments and the generic principles defined herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments. Those skilled in the art should appreciate that many modifications and variations are possible in light of the above teaching without departing from the scope of the invention.
Claims (6)
1. A method for changing fish body color distribution pattern, which is characterized by comprising the following steps: the geldanamycin analogue is given to incubate at the development stage of fish fertilized eggs;
the structural formula of the geldanamycin analogue is as follows:
2. the method of changing fish body color distribution pattern according to claim 1, wherein: the incubation adopts incubation water containing 5 +/-0.1 nmol/L of geldanamycin analogues.
3. The method of changing fish body color distribution pattern according to claim 1, wherein: the development stage of the fish fertilized eggs is the first cell stage of the fish fertilized eggs.
4. The method of changing fish body color distribution pattern according to claim 1, wherein: the incubation temperature is 28 +/-1 ℃, and the incubation time is 72 +/-1 h.
5. The method of changing fish body color distribution pattern according to claim 1, wherein: the geldanamycin analog is inhibited by administration of heat shock protein HSP 90.
6. The method for changing fish body color distribution pattern according to any one of claims 1 to 5, wherein: and breeding the hatched fry until the fry becomes sexually mature, then selfing, screening the fishes, then selfing and breeding, and further screening the offspring fishes with the body color distribution different from that of the parents.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911318002.4A CN110999828A (en) | 2019-12-19 | 2019-12-19 | Method for changing fish body color distribution pattern |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911318002.4A CN110999828A (en) | 2019-12-19 | 2019-12-19 | Method for changing fish body color distribution pattern |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110999828A true CN110999828A (en) | 2020-04-14 |
Family
ID=70117404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911318002.4A Pending CN110999828A (en) | 2019-12-19 | 2019-12-19 | Method for changing fish body color distribution pattern |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110999828A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2121957A1 (en) * | 2007-01-26 | 2009-11-25 | Kosan Biosciences, Inc. | Macrolactams by engineered biosynthesis |
| US20100068203A1 (en) * | 2006-05-09 | 2010-03-18 | Christine Martin | 17-Oxymacbecin Derivatives and Their Use in the Treatment of Cancer and/or B-Cell Malignancies |
| CN103609486A (en) * | 2013-10-28 | 2014-03-05 | 上海海洋大学 | Symphsodon discus breeding and fish larvae culturing method |
| CN105793421A (en) * | 2013-10-02 | 2016-07-20 | 大鹏药品工业株式会社 | Resistant mutant 90 kDa heat shock protein |
| CN107058386A (en) * | 2017-04-13 | 2017-08-18 | 厦门大学 | A kind of preparation method of transgenic zebrafish |
-
2019
- 2019-12-19 CN CN201911318002.4A patent/CN110999828A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100068203A1 (en) * | 2006-05-09 | 2010-03-18 | Christine Martin | 17-Oxymacbecin Derivatives and Their Use in the Treatment of Cancer and/or B-Cell Malignancies |
| EP2121957A1 (en) * | 2007-01-26 | 2009-11-25 | Kosan Biosciences, Inc. | Macrolactams by engineered biosynthesis |
| CN101688227A (en) * | 2007-01-26 | 2010-03-31 | 科森生物科学公司 | Macrocyclic lactams by through engineering approaches biosynthesizing preparation |
| CN105793421A (en) * | 2013-10-02 | 2016-07-20 | 大鹏药品工业株式会社 | Resistant mutant 90 kDa heat shock protein |
| CN103609486A (en) * | 2013-10-28 | 2014-03-05 | 上海海洋大学 | Symphsodon discus breeding and fish larvae culturing method |
| CN107058386A (en) * | 2017-04-13 | 2017-08-18 | 厦门大学 | A kind of preparation method of transgenic zebrafish |
Non-Patent Citations (2)
| Title |
|---|
| 姚静等: ""热休克蛋白90抑制剂的研究进展"", 《中国新药杂质》 * |
| 罗军涛: ""抑制热休克蛋白90(HSP90)功能对斑马鱼发育的影响"", 《中国优秀硕士学位论文全文数据库基础科学辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ohno | Evolution by gene duplication | |
| Kirpichnikov et al. | Selection of Krasnodar common carp (Cyprinus carpio L.) for resistance to dropsy: principal results and prospects | |
| Selvamani et al. | Microsatellite genotyping of individual abalone larvae: parentage assignment in aquaculture | |
| US9534235B2 (en) | Efficient sterilization of fish by disruption of germ cell development | |
| Kato | Breeding studies on red sea bream Pagrus major: mass selection to genome editing | |
| Spiess | Experimental population genetics | |
| Zhang et al. | Effect of water temperature on sex ratio and growth rate of juvenile Pelteobagrus fulvidraco, P. vachelli and hybrids [P. fulvidraco (♀)× P. vachelli (♂)] | |
| CN110999828A (en) | Method for changing fish body color distribution pattern | |
| KR20210022981A (en) | A composition for biofloc for aquaculture of sea horse and a method for aquaculture using the same | |
| Rivera et al. | Sexual Maturation in Farmed Atlantic Salmon (Salmo salar): A Review | |
| CN108575833A (en) | A kind of propagation method of crucian | |
| Sabbir et al. | Production of heterotic hybrid in Rohu (Labeo rohita) by crossing the riverine and hatchery strains | |
| US11624052B2 (en) | Functional sex-reversal of decapod crustacean females | |
| INAMORI et al. | ANALYSIS OF THE ENVIRONMENTAL FACTORS AFFECTING THE LIFE OF THE BRACKISH POLYCHAETE, NEANTHES JAPONIOA (IZUKA) II. THERMAL AND SALTY CONDITIONS REQUIRED FOR DEVELOPMENT AND GROWTH | |
| Hiruta et al. | How does the alteration of meiosis evolve to parthenogenesis?—Case study in a water flea, Daphnia pulex | |
| AU2016354255A1 (en) | A WW homogametic male decapod crustacean and methods of using the same | |
| Novel et al. | Critical points for the maintenance of genetic variability over a production cycle in the European sea bass, Dicentrarchus labrax | |
| JPH10327706A (en) | Breeding of flatfish using complete homozygote, produced flatfish and proliferation of flatfish | |
| CN107041326B (en) | Artificial breeding method of Eelia longissima | |
| Ayoola et al. | Biotechnology and species development in aquaculture | |
| Mito et al. | Cricket: The third domesticated insect | |
| Yona | Effect of culture temperature on rotifers (Brachionus plicatilis sensu strictu) size and reproduction activities | |
| CN116406649B (en) | Method for improving genetic diversity of oyster tetraploid and constructing tetraploid stable group line | |
| CN113207762B (en) | Method for cultivating gynogenesis megalobrama amblycephala pseudo-male fish by using high temperature | |
| KAwAMURA et al. | Interspecific hybrids between Japanese and European pond frogs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200414 |
|
| RJ01 | Rejection of invention patent application after publication |