CN110992368A - Culture dish imaging positioning method and device - Google Patents
Culture dish imaging positioning method and device Download PDFInfo
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- CN110992368A CN110992368A CN201911104838.4A CN201911104838A CN110992368A CN 110992368 A CN110992368 A CN 110992368A CN 201911104838 A CN201911104838 A CN 201911104838A CN 110992368 A CN110992368 A CN 110992368A
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000003384 imaging method Methods 0.000 title claims abstract description 26
- 238000004590 computer program Methods 0.000 claims description 6
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 9
- 230000000007 visual effect Effects 0.000 description 10
- 230000033001 locomotion Effects 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 2
- 210000000625 blastula Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/12—Edge-based segmentation
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/11—Region-based segmentation
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/60—Analysis of geometric attributes
- G06T7/66—Analysis of geometric attributes of image moments or centre of gravity
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/20—Special algorithmic details
- G06T2207/20048—Transform domain processing
- G06T2207/20061—Hough transform
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Abstract
The invention relates to the technical field of embryo culture imaging, and discloses a culture dish imaging positioning method and a culture dish imaging positioning device, wherein the method comprises the following steps: shooting a culture dish to obtain an original image, and obtaining a coarse positioning center of the culture dish according to the original image; judging whether the rough positioning center is positioned at the shooting view center, if not, adjusting the shooting view center to align the shooting view center with the rough positioning center, then reacquiring the original image, extracting the edge information of the culture dish according to the reacquired original image, and if so, directly extracting the dish edge information in the original image; fitting according to the dish edge information to obtain a fine positioning center of the culture dish; and intercepting an image area corresponding to the culture dish from the original image according to the fine positioning center. The invention can automatically complete the shooting position positioning of the culture dish imaging, and meanwhile, the culture dish image area positioning stability is high.
Description
Technical Field
The invention relates to the technical field of embryo culture imaging, in particular to a culture dish imaging positioning method and device.
Background
The time difference incubator provides great convenience for embryologists to observe the process of developing the fertilized eggs into the blastula, and also provides guarantee for the embryologists to select a basis for high-quality blastula transplantation. In the time difference incubator, a clear embryo image can be shot by an inverted microscope through a light source provided manually. However, in order to capture a complete embryo image, and also considering that embryos at different focal planes are slightly more distorted and errors may occur during the capturing process, a higher capturing resolution is usually set, for example 2048 × 1088. However, the embryo image with too large resolution is not convenient for embryologists to observe, and unnecessary resource waste is brought to the storage and transmission of the image. Due to the shooting angle and the reason of the development and growth of the embryo, sometimes a complete embryo image cannot be observed, but the culture dish where the embryo is located is always completely appeared in the shot image, so that in practical application, an embryologist makes a corresponding diagnosis by observing the information in the range of the culture dish area in the original image. How to automatically and quickly extract an image area of a culture dish from an original culture dish image with a large resolution stably and accurately, eliminate redundant information and reduce resource waste of storage and transmission is very important.
To ensure that the culture dish image can be captured by the inverted microscope, the embryologist needs to manually calibrate the dish to the center of the captured image when first capturing to ensure the integrity of the subsequent captured dish. However, the purely manual calibration method greatly increases the workload of the embryologist, and is also susceptible to machine errors caused by machine shake and the like in the subsequent shooting process. On the other hand, when observing embryo images continuously taken for multiple days, the embryologist needs to ensure the stability of the displayed images, that is, the offset of the displayed images of two adjacent frames is small. Although the prior art can obtain the position information of the dish, the prior art is easily interfered by noise in the image, so that the saved image cannot meet the requirement of an embryologist.
Disclosure of Invention
The invention aims to overcome the technical defects, provides a culture dish imaging and positioning method and device, and solves the technical problems that dish image extraction is interfered by noise and has low precision in the prior art.
In order to achieve the technical purpose, the technical scheme of the invention provides a culture dish imaging and positioning method, which comprises the following steps:
shooting a culture dish to obtain an original image, and obtaining a rough positioning center of the culture dish according to the original image;
judging whether the rough positioning center is positioned at a shooting view center, if not, adjusting the shooting view center to align the shooting view center with the rough positioning center, then re-acquiring the original image, extracting the edge information of the culture dish according to the re-acquired original image, and if so, directly extracting the dish edge information in the original image;
fitting according to the dish edge information to obtain a fine positioning center of the culture dish;
and intercepting an image area corresponding to the culture dish from the original image according to the fine positioning center.
The invention also provides a culture dish imaging and positioning device, which comprises a processor and a memory, wherein the memory is stored with a computer program, and the computer program is executed by the processor to realize the culture dish imaging and positioning method.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, the culture dish is subjected to two positioning processes, firstly, coarse positioning is carried out, the coarse positioning is used for judging whether the culture dish is positioned in the center of the shooting visual field, extraction of dish edge information is carried out after the culture dish is confirmed to be positioned in the center of the shooting visual field, the obtained original image is ensured to have a complete culture dish, subsequent secondary positioning is facilitated, manual eye observation is not needed for positioning, the influence of machine shake is avoided, the robustness of the machine error in the shooting process is enhanced, and the influence of irrelevant noise except the culture dish on the secondary positioning is eliminated. After extracting dish edge information, the accurate positioning of culture dish center is carried out according to the edge fitting, then according to accurate positioning center coordinate intercepting dish image, according to culture dish center intercepting dish image, can intercept accurate dish image to the culture dish center is carried out the interception and is made the change between the adjacent frame dish image little as the reference point, guarantees the stability of intercepting dish image.
Drawings
FIG. 1 is a flow chart of an embodiment of a method for positioning a culture dish for imaging according to the present invention;
FIG. 2 is an original image without coarse positioning adjustment and with a coarse positioning center in the image field of view;
FIG. 3 is an original image without coarse positioning adjustment and with the coarse positioning center not in the image field of view;
FIG. 4 is a truncated view of a dish image truncated from the fine positioning center;
fig. 5 is another cutout view of the dish image when the successive cutouts are made in accordance with the fine positioning center.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
As shown in fig. 1, embodiment 1 of the present invention provides a culture dish imaging positioning method, including the following steps:
s1, shooting a culture dish to obtain an original image, and obtaining a coarse positioning center of the culture dish according to the original image;
s2, judging whether the rough positioning center is located at a shooting view center, if not, adjusting the shooting view center to align the shooting view center with the rough positioning center, then reacquiring the original image, extracting the edge information of the culture dish according to the reacquired original image, and if so, directly extracting the edge information of the dish in the original image;
s3, fitting according to the dish edge information to obtain a fine positioning center of the culture dish;
and S4, intercepting an image area corresponding to the culture dish from the original image according to the fine positioning center.
The culture dish imaging positioning method provided by the embodiment of the invention divides the positioning of the culture dish in the image into two steps: and (4) coarse positioning and fine positioning, namely, positioning twice. It should be understood that the coarse and fine positioning in the present invention are relative concepts of qualitative and not quantitative. The main purpose of coarse positioning is to position the shooting field, and when the manual calibration of the shooting field is not accurate or calibrated, only part of the information of the culture dish can be observed in the shot original image, or the culture dish is not positioned in the center of the original image. In order to ensure that the culture dish is positioned at the center of the shooting visual field to obtain a complete culture dish area image, a coarse positioning center of the culture dish is firstly obtained so as to judge whether the culture dish is positioned at the center of the shooting visual field according to the coarse positioning center, and secondary fine positioning can be carried out only when the center of the culture dish is confirmed to be positioned at the center of the shooting visual field. Secondary positioning, accurate positioning promptly in this embodiment, at first extract ware marginal information, then the accurate positioning center is solved in the fitting, according to accurate positioning center intercepting ware image, because ware image is according to accurate positioning center intercepting, consequently make the center of ware image align with the center of culture dish to stability when having guaranteed the continuous intercepting of ware image.
Preferably, the obtaining of the rough positioning center of the culture dish according to the original image specifically includes:
and obtaining the region information of the culture dish in the original image through Hough transform according to the shape of the culture dish, and solving the coarse positioning center according to the region information.
The purpose of coarse positioning in the invention is to ensure that the center of the shooting visual field of the original image is aligned with the center of the culture dish, and the center of the shooting visual field, namely the center of the original image, ensures the consistency of the shot original image, therefore, the coarse positioning has low requirement on the positioning precision, and the conventional arbitrary positioning method can be adopted. In the embodiment, a hough transform is adopted to obtain a coarse positioning center, the hough transform is one of basic methods for identifying geometric shapes from images in image processing, and the culture dish is circular in the embodiment, so that a circular area is identified from an original image through the hough transform, and the area information where the culture dish is located can be obtained, and then the center of the area information is obtained, so that the coarse positioning center can be obtained.
Preferably, the acquiring of the rough positioning center of the culture dish according to the original image further includes:
and if the area information is incomplete, expanding the edge of the area information according to the shape of the culture dish, and solving the coarse positioning center according to the expanded area information.
When the original image shot for the first time contains a complete culture dish, coarse positioning can be directly carried out, however, if only a small number of regions of the culture dish are contained, for example, only a small number of culture dish regions are contained in fig. 3, the coarse positioning center solved under the condition is not located in the range of the original image, at this time, region information must be expanded, namely the region information is supplemented into a complete circle, the coarse positioning center is calculated according to the expanded region information, the shooting adjusting process based on the coarse positioning is completed according to the relative position of the coarse positioning center and the shooting view center, and the original image of the culture dish center located in the image center is ensured to be obtained.
Preferably, if the rough positioning center is not located at the photographing view center, the photographing view center is adjusted so that the photographing view center is aligned with the rough positioning center, then the original image is re-acquired, and the vessel edge information is extracted from the re-acquired original image.
If the coarse positioning center is not located at the center of the shooting visual field, the center of the shooting visual field needs to be adjusted, so that the original image shot is ensured to be aligned with the center of the culture dish. Fig. 2 shows an original image in which the center of the field of view is not captured and the coarse positioning center is located within the field of view, while fig. 3 shows an original image in which the center of the field of view is not captured and the coarse positioning center is not located within the field of view. Through the shooting position of the microscope, the integrity of the culture dish image is guaranteed in the visual field of the shot original image, and as can be understood, the adjustment of the shooting position of the microscope can be realized on hardware through the existing three-axis motion platform, and the microscope is installed on the three-axis motion platform, so that the automatic movement of the microscope can be realized.
Judging whether the coarse positioning center is positioned at the center of the shooting view field, specifically:
and judging whether the distance between the coarse positioning center and the shooting view center is smaller than a set threshold value, if so, judging that the coarse positioning center is positioned in the shooting view center, and if not, judging that the coarse positioning center is not positioned in the shooting view center.
FIG. 4 is a diagram of the vessel image in the original image cut out in proportion to the fine positioning center point. Fig. 5 is another cutout view of the dish image when the successive cutouts are made in accordance with the fine positioning center.
Example 2
Embodiment 2 of the present invention provides a culture dish imaging and positioning apparatus, which includes a processor and a memory, where the memory stores a computer program, and the computer program is executed by the processor to implement the culture dish imaging and positioning method provided in the above embodiments.
The culture dish imaging and positioning device provided by the embodiment of the invention is used for realizing the culture dish imaging and positioning method, so that the culture dish imaging and positioning method has the technical effects, and the culture dish imaging and positioning device also has the technical effects, and is not described herein again.
The above-described embodiments of the present invention should not be construed as limiting the scope of the present invention. Any other corresponding changes and modifications made according to the technical idea of the present invention should be included in the protection scope of the claims of the present invention.
Claims (4)
1. A culture dish imaging positioning method is characterized by comprising the following steps:
shooting a culture dish to obtain an original image, and obtaining a rough positioning center of the culture dish according to the original image;
judging whether the rough positioning center is positioned at a shooting view center, if not, adjusting the shooting view center to align the shooting view center with the rough positioning center, then re-acquiring the original image, extracting the edge information of the culture dish according to the re-acquired original image, and if so, directly extracting the dish edge information in the original image;
fitting according to the dish edge information to obtain a fine positioning center of the culture dish;
and intercepting an image area corresponding to the culture dish from the original image according to the fine positioning center.
2. The culture dish imaging positioning method according to claim 1, wherein the obtaining of the rough positioning center of the culture dish according to the original image comprises:
and obtaining the region information of the culture dish in the original image through Hough transform according to the shape of the culture dish, and solving the coarse positioning center according to the region information.
3. The culture dish imaging positioning method according to claim 2, wherein the obtaining of the rough positioning center of the culture dish according to the original image further comprises:
and if the area information is incomplete, expanding the edge of the area information according to the shape of the culture dish, and solving the coarse positioning center according to the expanded area information.
4. An imaging positioning apparatus for a culture dish, comprising a processor and a memory, wherein the memory stores a computer program, and the computer program is executed by the processor to implement the imaging positioning method for the culture dish according to any one of claims 1 to 3.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911104838.4A CN110992368A (en) | 2019-11-08 | 2019-11-08 | Culture dish imaging positioning method and device |
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| CN201911104838.4A CN110992368A (en) | 2019-11-08 | 2019-11-08 | Culture dish imaging positioning method and device |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150278575A1 (en) * | 2012-11-07 | 2015-10-01 | bioMérieux | Bio-imaging method |
| WO2017215351A1 (en) * | 2016-06-15 | 2017-12-21 | 珠海格力电器股份有限公司 | Method and apparatus for adjusting recognition range of photographing apparatus |
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- 2019-11-08 CN CN201911104838.4A patent/CN110992368A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150278575A1 (en) * | 2012-11-07 | 2015-10-01 | bioMérieux | Bio-imaging method |
| WO2017215351A1 (en) * | 2016-06-15 | 2017-12-21 | 珠海格力电器股份有限公司 | Method and apparatus for adjusting recognition range of photographing apparatus |
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Application publication date: 20200410 |