CN110988178A - High performance liquid chromatography detection method for phosphorylcholine - Google Patents
High performance liquid chromatography detection method for phosphorylcholine Download PDFInfo
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- CN110988178A CN110988178A CN201911299590.1A CN201911299590A CN110988178A CN 110988178 A CN110988178 A CN 110988178A CN 201911299590 A CN201911299590 A CN 201911299590A CN 110988178 A CN110988178 A CN 110988178A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
- G01N2030/047—Standards external
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Abstract
The invention belongs to the technical field of analytical chemistry, and relates to a high performance liquid chromatography detection method of phosphorylcholine. Separating the sample liquid by a neutral amide bonded phase chromatographic column, isocratically eluting acetonitrile and 0.1 percent formic acid solution, and detecting by an evaporative light detector. The method is simple and convenient, can quickly measure the content of the phosphorylcholine, and has higher accuracy, stability and separation degree.
Description
Technical Field
The invention belongs to the technical field of analytical chemistry, and relates to a high performance liquid chromatography detection method of phosphorylcholine, in particular to a high performance liquid chromatography detection method of phosphorylcholine, which has high accuracy, stability and resolution and is used for neutral amide bonded phase chromatographic column separation and evaporative light detector detection.
Background
The phosphorylcholine exists in the form of chlorinated phosphorylcholine hydrochloride, can participate in synthesizing phospholipid, has the effects of protecting liver, strengthening liver, promoting lipid metabolism and resisting fatty liver, can accelerate methyl transfer and promote liver cell regeneration, and can synthesize acetylcholine in vivo to activate nervous system; decompose histamine, enhance the activity of renal histamine enzyme, and has the function of detoxification, and meanwhile, the phosphorylcholine is also a raw material for synthesizing phosphorylcholine morpholine which is an intermediate of choline medicaments. The phosphorylcholine can be used in the field of medicine, can also be used as a quality modifier for brewed products, and can stabilize amino acid and other wine ingredients.
At present, the content of choline phosphate in the beverage is detected by using a liquid chromatography-mass spectrometry method, but the detection cost of the liquid chromatography-mass spectrometry method is higher, and the universality of the liquid chromatography is not strong, so a high performance liquid chromatography detection method for the choline phosphate is required to be established.
The method is simple to operate, can quickly determine the content of the phosphorylcholine, and has higher accuracy, stability and separation degree.
Disclosure of Invention
The invention aims to provide high performance liquid chromatography detection with higher accuracy, stability and separation degree, and the high performance liquid chromatography detection can realize the rapid determination of the content of the phosphorylcholine by the separation of a neutral amido bonded phase chromatographic column and the detection of an evaporative light detector.
In order to realize the purpose of the invention, the technical scheme of the invention is as follows: dissolving a sample by pure water or methanol, fixing the volume, injecting the sample into a high performance liquid chromatograph after passing through a membrane, collecting a chromatogram under the condition of setting an instrument, and quantifying by an external standard method; wherein, the instrument conditions are set as that the neutral amido bonded phase chromatographic column is separated, the column temperature is 35 ℃, acetonitrile with the volume ratio of 20/80 and 0.1 percent formic acid solution are used as mobile phases for isocratic elution, the column flow is 0.5mL/min, the sample injection volume is 10 mu L, and the detection is carried out by an evaporative light detector.
Has the advantages that: the method adopts neutral amide bonded phase chromatographic column separation and an evaporative light detector for detection, has higher accuracy, stability and separation degree, is simple to operate, can quickly determine the content of the phosphorylcholine, and greatly saves the analysis time and cost.
Drawings
FIG. 1 high performance liquid chromatogram of standard solution
Detailed Description
The present invention is further illustrated by the following examples, but is not limited thereto in any way, and any variations or modifications based on the teachings of the present invention are within the scope of the present invention.
Example one
The high performance liquid chromatograph is matched with an evaporation light detector.
0.1g of the phosphorylcholine standard substance (precisely 0.001g) was weighed out accurately, dissolved in methanol and made to 100 mL. The chromatographic conditions employed were: venusil HILIC chromatography column; column temperature of 35 ℃; acetonitrile and 0.1% formic acid solution are eluted with equal degree by mobile phase and have the volume ratio of 20/80; flow rate of 0.5 mL/min; sample introduction volume of 10 μ L; and detecting by an evaporative light detector. And (4) injecting the standard solution into a high performance liquid chromatograph after passing through a membrane, and recording a chromatogram. 3.75min is a phosphorylcholine spectrum peak, the retention time is appropriate, the separation degree is high, and the peak shape symmetry is good.
Claims (1)
1. A high performance liquid chromatography detection method of phosphorylcholine is characterized in that a sample is dissolved by pure water or methanol and subjected to constant volume, the solution is injected into a high performance liquid chromatograph after passing through a membrane, a chromatogram is collected under the condition of setting an instrument, and the quantification is carried out by an external standard method; wherein, the instrument conditions are set as that the neutral amido bonded phase chromatographic column is separated, the column temperature is 35 ℃, acetonitrile with the volume ratio of 20/80 and 0.1 percent formic acid solution are used as mobile phases for isocratic elution, the column flow is 0.5mL/min, the sample injection volume is 10 mu L, and the detection is carried out by an evaporative light detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201911299590.1A CN110988178A (en) | 2019-12-12 | 2019-12-12 | High performance liquid chromatography detection method for phosphorylcholine |
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| CN201911299590.1A CN110988178A (en) | 2019-12-12 | 2019-12-12 | High performance liquid chromatography detection method for phosphorylcholine |
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| CN110988178A true CN110988178A (en) | 2020-04-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116879428A (en) * | 2023-06-29 | 2023-10-13 | 沈阳金久奇科技有限公司 | High performance liquid analysis method for residual content of phosphorylcholine in L-alpha-phosphorylcholine |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5643716A (en) * | 1992-09-08 | 1997-07-01 | Amur Research Corp. | Diagnostic agent and methods for identifying HIV infected individuals and monitoring their therapy |
| CN102138892A (en) * | 2010-02-03 | 2011-08-03 | 广州汉光医药进出口有限公司 | Choline alfoscerate injection preparation as well as preparation method and detection method thereof |
| CN105388225A (en) * | 2015-10-23 | 2016-03-09 | 苏州天马医药集团天吉生物制药有限公司 | Analysis and detection method for UDPC in medicinal preparation containing citicoline sodium |
| CN109709220A (en) * | 2017-10-25 | 2019-05-03 | 中国科学院大连化学物理研究所 | It is a kind of for the joint marker and kit of diagnosing bladder cancer and application |
-
2019
- 2019-12-12 CN CN201911299590.1A patent/CN110988178A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5643716A (en) * | 1992-09-08 | 1997-07-01 | Amur Research Corp. | Diagnostic agent and methods for identifying HIV infected individuals and monitoring their therapy |
| CN102138892A (en) * | 2010-02-03 | 2011-08-03 | 广州汉光医药进出口有限公司 | Choline alfoscerate injection preparation as well as preparation method and detection method thereof |
| CN105388225A (en) * | 2015-10-23 | 2016-03-09 | 苏州天马医药集团天吉生物制药有限公司 | Analysis and detection method for UDPC in medicinal preparation containing citicoline sodium |
| CN109709220A (en) * | 2017-10-25 | 2019-05-03 | 中国科学院大连化学物理研究所 | It is a kind of for the joint marker and kit of diagnosing bladder cancer and application |
Non-Patent Citations (2)
| Title |
|---|
| 中华人民共和国国家质量监督检验检疫总局: "《中华人民共和国出入境检验检疫行业标准SN/T 3937-2014》", 9 April 2014 * |
| 杜章斌等: "HPLC-ELSD法测定酶解磷脂中甘油磷脂酰胆碱含量的研究", 《粮食与食品工业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116879428A (en) * | 2023-06-29 | 2023-10-13 | 沈阳金久奇科技有限公司 | High performance liquid analysis method for residual content of phosphorylcholine in L-alpha-phosphorylcholine |
| CN116879428B (en) * | 2023-06-29 | 2024-04-02 | 沈阳金久奇科技有限公司 | High performance liquid analysis method for residual content of phosphorylcholine in L-alpha-phosphorylcholine |
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Application publication date: 20200410 |