CN110987552A - In vitro animal skin sample preparation equipment - Google Patents
In vitro animal skin sample preparation equipment Download PDFInfo
- Publication number
- CN110987552A CN110987552A CN201911101456.6A CN201911101456A CN110987552A CN 110987552 A CN110987552 A CN 110987552A CN 201911101456 A CN201911101456 A CN 201911101456A CN 110987552 A CN110987552 A CN 110987552A
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- China
- Prior art keywords
- temporary storage
- cooling chamber
- storage cup
- cutter
- cutter seat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
Abstract
The invention discloses an in vitro animal skin sample preparation device, which comprises: the device comprises a refrigeration mechanism, an operation table, a cutting mechanism and a pressing mechanism; the operating platform and the cutting mechanism are arranged on the refrigerating mechanism; the pressing mechanism is installed on the cutting mechanism. The invention can rapidly cut the in vitro skin of the animal at one time, reduce the sample preparation time and effectively improve the experimental efficiency; the prepared experimental material has good size consistency, can reduce the waste of the in-vitro skin of the animal and improve the utilization rate of the experimental material; in addition, the cut experimental materials are directly soaked in the preservation solution for refrigeration, so that the pollution to the experimental materials can be effectively reduced.
Description
Technical Field
The invention relates to the field of sample preparation equipment, in particular to in-vitro animal skin sample preparation equipment.
Background
With the development of scientific technology, the technology of the animal in-vitro skin corrosion test is applied to evaluate the corrosion potential of chemicals on the skin and gradually replaces the traditional animal in-vivo test.
At present, sample preparation equipment special for in-vitro animal skin does not exist, and in experiments, experimenters mostly adopt simple tools to manually prepare samples to cut and cut animal skin samples, such as scalpels and surgical scissors. When a simple tool is used for sample preparation, experimenters are easy to be tired, the sample preparation time is long, the efficiency is low, the consistency of sample specifications cannot be guaranteed, and the waste and the pollution of experimental materials are easily caused.
Disclosure of Invention
The invention mainly solves the problems in the prior art and provides sample preparation equipment which can cut and cut the in-vitro skin of an animal efficiently and carry out cold storage and temporary storage simultaneously.
The invention is realized by the following technologies:
an ex vivo animal skin sampling device comprising: the device comprises a refrigeration mechanism, an operation table, a cutting mechanism and a pressing mechanism; the operating platform and the cutting mechanism are arranged on the refrigerating mechanism; the pressing mechanism is arranged on the cutting mechanism;
the cold storage mechanism includes: the temperature display screen, the control box, the temperature sensor and the cooling chamber are arranged on the cooling chamber; the top of the cooling chamber is provided with a cooling hole which is communicated with the inside of the cooling chamber; the temperature sensor is arranged inside the cooling chamber; the control box is arranged on the cooling chamber; the temperature display screen is arranged on the control box; the temperature display screen, the temperature sensor and the control box are electrically connected;
the operation panel includes: the sample temporary storage cup comprises a base plate, a sample temporary storage cup and a temporary storage cup fixing plate; the temporary storage cup fixing plate is arranged on the cooling chamber; the temporary storage cup fixing plate is provided with a clamping groove; the clamping groove is arranged above the cooling hole; the sample temporary storage cup is arranged on the clamping groove; the backing plate is arranged on the temporary storage cup fixing plate, a collecting hole is formed in the backing plate, and the collecting hole is formed above the temporary storage cup;
the cutting mechanism includes: the device comprises a main shaft, a cutter seat, a cutter and a handle; the main shaft is arranged on the cooling chamber; the cutter seat is arranged on the main shaft; the cutter and the handle are arranged on the cutter seat, and the cutter is positioned above the collecting hole;
the hold-down mechanism includes: the limiting screw, the first spring and the compression ring; the compression ring is sleeved on the outer side of the cutter; the clamp ring is installed on the cutter seat through a limiting screw, and a first spring is arranged on the limiting screw.
Further, a fixing block is mounted on the main shaft and fixed above the cutter seat; and a second spring is connected between the fixed block and the cutter seat.
Furthermore, a guide hole is formed in the fixed block; and a guide rail is arranged in the guide hole and is connected with the cutter seat.
Further, the handle is hinged to the side faces of the fixing block and the cutter seat.
Further, a cooler is installed inside the cooling chamber, and the cooler is electrically connected with the control box.
The use method of the invention comprises the following steps:
1. opening the base plate, taking out the temporary storage cup and the temporary storage cup fixing plate, injecting a proper amount of cooling liquid into the cooling chamber, injecting a proper amount of sample preservation liquid into the temporary storage cup, and then sequentially installing the temporary storage cup fixing plate, the temporary storage cup and the base plate again;
2. turning on a power switch on the control box, and starting the cooler;
3. the temperature of the cooling liquid in the cooling chamber is set through the control box;
4. selecting cutters with corresponding sizes to be installed on the cutter seat according to actual requirements;
5. the position of the cutting mechanism on the main shaft is adjusted and fixed, so that the cutting action can be normally implemented;
6. observing the temperature display screen, wherein the temperature of the chamber to be cooled is stable;
7. after rinsing, sterilizing and disinfecting the in-vitro animal skin, placing the in-vitro animal skin on a base plate, pressing an operating handle downwards to drive a cutter seat, a cutter and a pressing mechanism to move downwards along the direction of a main shaft, firstly contacting the in-vitro animal skin by a pressing ring and pressing the in-vitro animal skin tightly on the base plate, then contacting the in-vitro animal skin by the cutter and continuing to move downwards to execute cutting action, stopping pressing the handle downwards after cutting off, driving the cutter seat, the cutter and the pressing mechanism to reset under the action of a spring, and receiving a cut in-vitro animal skin sample in a sample temporary storage cup through a collection hole;
8. after the animal in-vitro skin samples are cut in batches, the backing plate is opened, the sample temporary storage cup is taken out, and the processed samples are transferred to refrigeration or freezing for standby.
The invention has the beneficial effects that:
1. in the invention, experimenters can rapidly cut the in-vitro skin of the animal at one time, so that the sample preparation time is reduced, and the experimental efficiency is effectively improved;
2. the prepared experimental material has good size consistency, can reduce the waste of the in-vitro skin of the animal and improve the utilization rate of the experimental material;
3. the cut experimental material is directly soaked in the preservation solution for refrigeration, so that the pollution to the experimental material can be effectively reduced.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a structural use and intent of the console of the present invention.
Fig. 3 is a schematic structural diagram of the cutting mechanism of the present invention.
Fig. 4 is an exploded view of the cutting mechanism of the present invention.
Reference signs mean: 1. a refrigeration mechanism; 101. a cooling chamber; 102. a control box; 103. a temperature display screen; 104. a cooling hole; 2. an operation table; 201. a base plate; 202. a temporary storage cup fixing plate; 203. a sample temporary storage cup; 204. a card slot; 205. a collection well; 3. a cutting mechanism; 301. a main shaft; 302. a tool holder; 303. a cutter; 304. a handle; 305. a fixed block; 306. a second spring; 307. a guide hole; 308. a guide rail; 309. a hinge plate; 4. a hold-down mechanism; 401. a compression ring; 402. a limiting screw; 403. and a first spring.
Detailed Description
In order to make the technical solution, object and advantages of the present invention more apparent, the present invention will be further explained with reference to the accompanying drawings and embodiments.
An ex vivo animal skin sampling device comprising: the refrigerator comprises a refrigeration mechanism 1, an operation table 2, a cutting mechanism 3 and a pressing mechanism 4; the operating platform 2 and the cutting mechanism 3 are arranged on the top end of the refrigerating mechanism 1; the pressing mechanism 4 is arranged on the cutting mechanism 3;
the refrigeration mechanism 1 includes: a temperature display screen 103, a control box 102, a temperature sensor (not marked in the figure) and a cooling chamber 101; the top of the cooling chamber 101 is provided with a cooling hole 104, the cooling hole 104 is communicated with the inside of the cooling chamber 101, and positioning pins are arranged around the cooling hole 104; a temperature sensor installed inside the cooling chamber 101 for detecting the temperature inside the cooling chamber 101; a cooler (not shown) is installed inside the cooling chamber 101, and can maintain the cooling liquid in the cooling chamber 101 at a desired temperature; the control box 102 is installed on the top of the cooling chamber 101; the temperature display screen 103 is arranged on the control box 102; the temperature display screen 103, the temperature sensor and the cooler are respectively electrically connected with the control box 102, the temperature sensor transmits temperature information to the control box 102 and displays the temperature information on the temperature display screen 103, and the action of the cooler is controlled through the control box 102 to adjust the temperature to the required temperature;
the operation table 2 includes: a backing plate 201, a temporary sample storage cup 203 and a temporary storage cup fixing plate 202; a positioning hole matched with the positioning pin is formed in the temporary storage cup fixing plate 202, and the temporary storage cup fixing plate 202 is fixed on the cooling hole 104 through the positioning pin; the temporary storage cup fixing plate 202 is provided with a clamping groove 204, the diameter of the clamping groove 204 is smaller than the upper edge of the temporary storage cup 203, so that the lower part of the temporary storage cup 203 can be placed in the clamping groove 204, and the upper edge part of the temporary storage cup 203 is clamped on the clamping groove 204; the clamping groove 204 is arranged above the cooling hole 104, so that the lower part of the sample temporary storage cup 203 can enter the cooling chamber 101 to be in contact with the cooling liquid in the cooling chamber 101; the backing plate 201 covers the temporary storage cup fixing plate 202, a collecting hole 205 is formed in the backing plate 201, the collecting hole 205 is formed above the temporary storage cup 203 of the sample, the diameter of the collecting hole 205 is smaller than that of the temporary storage cup 203 of the sample, and therefore the sample can be conveniently cut and enter the temporary storage cup 203 of the sample;
the cutting mechanism 3 includes: a main shaft 301, a tool holder 302, a tool 303 and a handle 304; the main shaft 301 is installed on the top end of the cooling chamber 101; the cutter seat 302 is arranged on the main shaft 301, and the cutter seat 302 can move up and down on the main shaft 301; a boss extends outwards from the cutter seat 302, and a cutter 303 is arranged at the bottom of the boss and is positioned right above the collecting hole 205; the handle 304 is mounted on the tool holder 302; moving a handle 304, wherein the handle 304 drives a cutter seat 302 to move on the main shaft 301;
the pressing mechanism 4 includes: a limit screw 402, a spring I403 and a compression ring 401; the clamp ring 401 is sleeved on the outer side of the cutter 303; the compression ring 401 is mounted on a boss of the cutter seat 302 through a limit screw 402, and a first spring 403 is arranged on the limit screw 402; the limit screw 402 and the first spring 403 are used together, so that the pressing mechanism 4 only performs a pressing action, and the movement of the tool base 302 and the tool 303 is not influenced.
Further, a fixing block 305 is mounted on the main shaft 301, and the fixing block 305 is fixed above the cutter seat 302; a second spring 306 is arranged between the fixed block 305 and the cutter seat 302; the fixed block 305 is fixed, the cutter seat 302 moves downwards, the cutter 303 cuts a sample, the second spring 306 stretches, and after cutting is finished, the second spring 306 contracts to drive the cutter seat 302 to move upwards to an initial position; the fixed block 305 is provided with a guide hole 307 penetrating through the fixed block 305, the top end of the cutter seat 302 is provided with a threaded hole, the guide rail 308 penetrates through the guide hole 307 and is screwed in the threaded hole of the cutter seat 302, and the movement of the cutter seat 302 is ensured not to be deviated through the matching of the guide rail 308 and the guide hole 307; one end of the handle 304 is hinged on the side of the fixed block 305, and then hinged on the side of the cutter seat 302 by using a hinged plate 309, when the handle 304 is pressed down, the handle 304 pushes the hinged plate 309, and the hinged plate 309 pushes the cutter seat 302 to move downwards.
The using method comprises the following steps:
1. opening the backing plate 202, taking out the temporary sample storage cup 203 and the temporary sample storage cup fixing plate 202, injecting a proper amount of cooling liquid into the cooling chamber 101, injecting a proper amount of sample preservation liquid into the temporary sample storage cup 203, and then installing the temporary sample storage cup fixing plate 202, the temporary sample storage cup 203 and the backing plate 201 in sequence;
2. turning on a power switch on the control box 102 to start the cooler;
3. the temperature of the cooling liquid in the cooling chamber 101 is set by the control box 102;
4. selecting a cutter 303 with a corresponding size to be mounted on the cutter seat 302 according to actual needs;
5. adjusting the position of the cutter seat 302 on the main shaft 301 to make the cutter 303 align with the collecting hole 205, so as to ensure that the cutting action can be normally implemented;
6. observing the temperature display screen 103, wherein the temperature of the chamber to be cooled 101 is stable;
7. after rinsing, sterilizing and disinfecting the in-vitro skin of the animal, placing the in-vitro skin of the animal on the backing plate 201, pressing the operating handle 304 downwards to drive the cutter seat 302, the cutter 303 and the pressing mechanism 4 to move downwards along the direction of the main shaft 301, firstly, the pressing ring 401 contacts the in-vitro skin of the animal and tightly presses the in-vitro skin of the animal on the backing plate 301, then, the cutter seat 302 continues to move downwards, the cutter 303 contacts the in-vitro skin of the animal and continues to move downwards to execute cutting action, the handle 304 stops pressing after being cut off, the cutter seat 302, the cutter 303 and the pressing mechanism 4 are driven to reset under the action of the first spring 403 and the second spring 306, and the cut in-vitro skin sample of the animal is received in the;
8. after the animal in vitro skin samples are cut in batches, the backing plate 201 is opened, the sample temporary storage cup 203 is taken out, and the processed samples are transferred to refrigeration or freezing for standby.
The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention accordingly, and not to limit the protection scope of the present invention accordingly. All equivalent changes or modifications made in accordance with the spirit of the present disclosure are intended to be covered by the scope of the present disclosure.
Claims (5)
1. An ex vivo animal skin sampling device comprising: the device comprises a refrigeration mechanism, an operation table, a cutting mechanism and a pressing mechanism; the operating platform and the cutting mechanism are arranged on the refrigerating mechanism; the pressing mechanism is arranged on the cutting mechanism; the method is characterized in that:
the cold storage mechanism includes: the temperature display screen, the control box, the temperature sensor and the cooling chamber are arranged on the cooling chamber; the top of the cooling chamber is provided with a cooling hole which is communicated with the inside of the cooling chamber; the temperature sensor is arranged inside the cooling chamber; the control box is arranged on the cooling chamber; the temperature display screen is arranged on the control box; the temperature display screen, the temperature sensor and the control box are electrically connected;
the operation panel includes: the sample temporary storage cup comprises a base plate, a sample temporary storage cup and a temporary storage cup fixing plate; the temporary storage cup fixing plate is arranged on the cooling chamber; the temporary storage cup fixing plate is provided with a clamping groove; the clamping groove is arranged above the cooling hole; the sample temporary storage cup is arranged on the clamping groove; the backing plate is arranged on the temporary storage cup fixing plate, a collecting hole is formed in the backing plate, and the collecting hole is formed above the temporary storage cup;
the cutting mechanism includes: the device comprises a main shaft, a cutter seat, a cutter and a handle; the main shaft is arranged on the cooling chamber; the cutter seat is arranged on the main shaft; the cutter and the handle are arranged on the cutter seat, and the cutter is positioned above the collecting hole;
the hold-down mechanism includes: the limiting screw, the first spring and the compression ring; the compression ring is sleeved on the outer side of the cutter; the clamp ring is installed on the cutter seat through a limiting screw, and a first spring is arranged on the limiting screw.
2. The isolated animal skin sampling device of claim 1, wherein: the main shaft is provided with a fixed block, and the fixed block is fixed above the cutter seat; and a second spring is connected between the fixed block and the cutter seat.
3. The isolated animal skin sampling device of claim 2, wherein: the fixed block is provided with a guide hole; and a guide rail is arranged in the guide hole and is connected with the cutter seat.
4. The isolated animal skin sampling device of claim 2 or 3, wherein: the handle is hinged on the side surfaces of the fixing block and the cutter seat.
5. The isolated animal skin sampling device of claim 1, wherein: the inside of cooling chamber installs the cooler, cooler and control box electric connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911101456.6A CN110987552B (en) | 2019-11-12 | 2019-11-12 | In vitro animal skin sample preparation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911101456.6A CN110987552B (en) | 2019-11-12 | 2019-11-12 | In vitro animal skin sample preparation equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110987552A true CN110987552A (en) | 2020-04-10 |
| CN110987552B CN110987552B (en) | 2022-03-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911101456.6A Active CN110987552B (en) | 2019-11-12 | 2019-11-12 | In vitro animal skin sample preparation equipment |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6048353A (en) * | 1999-03-04 | 2000-04-11 | L. Vad Technology, Inc. | Trephine device for locating and cutting a cylindrical or reverse tapered aperture in flexible material |
| US20050044971A1 (en) * | 2003-09-02 | 2005-03-03 | Harris Joel Steven | Motor driven sampling apparatus for material collection |
| US20050078471A1 (en) * | 2003-10-07 | 2005-04-14 | Leica Mikrosysteme Gmbh | Cooling chamber and system of a cooling chamber with a microtome |
| CN103185673A (en) * | 2011-12-30 | 2013-07-03 | 李朝林 | Integrative box type multifunctional histotome |
| CN106353127A (en) * | 2016-10-28 | 2017-01-25 | 广东省中医院 | Synchronous slicing device for tissues |
| CN106525524A (en) * | 2016-10-13 | 2017-03-22 | 中国人民解放军国防科学技术大学 | Apparatus for preparing brain tissue sample and preparation method thereof |
| CN209289366U (en) * | 2018-12-26 | 2019-08-23 | 上海正诚机电制造有限公司 | A kind of trotter cutter device |
-
2019
- 2019-11-12 CN CN201911101456.6A patent/CN110987552B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6048353A (en) * | 1999-03-04 | 2000-04-11 | L. Vad Technology, Inc. | Trephine device for locating and cutting a cylindrical or reverse tapered aperture in flexible material |
| US20050044971A1 (en) * | 2003-09-02 | 2005-03-03 | Harris Joel Steven | Motor driven sampling apparatus for material collection |
| US20050078471A1 (en) * | 2003-10-07 | 2005-04-14 | Leica Mikrosysteme Gmbh | Cooling chamber and system of a cooling chamber with a microtome |
| CN103185673A (en) * | 2011-12-30 | 2013-07-03 | 李朝林 | Integrative box type multifunctional histotome |
| CN106525524A (en) * | 2016-10-13 | 2017-03-22 | 中国人民解放军国防科学技术大学 | Apparatus for preparing brain tissue sample and preparation method thereof |
| CN106353127A (en) * | 2016-10-28 | 2017-01-25 | 广东省中医院 | Synchronous slicing device for tissues |
| CN209289366U (en) * | 2018-12-26 | 2019-08-23 | 上海正诚机电制造有限公司 | A kind of trotter cutter device |
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| Publication number | Publication date |
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| CN110987552B (en) | 2022-03-25 |
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Address after: 510070 Guangzhou City, Guangzhou, Guangdong, No. 34 Patentee after: Institute of testing and analysis, Guangdong Academy of Sciences (Guangzhou analysis and testing center, China) Address before: 510070 Guangzhou City, Guangzhou, Guangdong, No. 34 Patentee before: GUANGDONG INSTITUTE OF ANALYSIS (CHINA NATIONAL ANALYTICAL CENTER, GUANGZHOU) |
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