CN110982809A - Microbial microsphere carrier for treating various environmental pollutants and preparation method thereof - Google Patents
Microbial microsphere carrier for treating various environmental pollutants and preparation method thereof Download PDFInfo
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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Abstract
The invention discloses a microbial microsphere carrier for treating various environmental pollutants and a preparation method thereof, which relate to the field of environmental pollutant treatment and comprise the following raw materials: the method comprises the following steps of uniformly mixing 18 parts by mass of raw material powder, 1 part by volume of a microbial preparation I and 1 part by mass of a preparation II, obtaining a microsphere carrier mixture, dissolving 5 parts by mass of the microsphere carrier mixture in 25 parts by volume of deionized water, stirring at 21-26 ℃ for 1 hour at a speed of 80-220rpm to obtain an initial mixture, measuring by using a pH meter, and adjusting the pH of the initial mixture to be neutral by using a 1M citric acid solution; and preparing the mixture with the well-mixed pH into round granules with the diameter of 2-5 mm by using a non-heating granulator, and culturing for 1 week at the temperature of 30 ℃ to obtain the microsphere carrier.
Description
Technical Field
The invention relates to the field of environmental pollutant treatment, in particular to a microbial microsphere carrier for treating various environmental pollutants and a preparation method thereof.
Background
Microorganisms are a key element of the natural ecosystem and material circulation. The rapid development of technology has proven that microorganisms are the most ideal solution for environmental protection. How to apply the microorganisms screened from the laboratory to the practical environmental protection application has become the direction of the key research and development in China. The development of the microbial carrier is one of the methods for solving the environmental pollution by applying the research results of the laboratory to the engineering. Many existing suspended microbial carrier fillers for pollutant treatment need to be improved due to risks and reasons such as relatively expensive, non-environmentally friendly raw materials and the like.
Disclosure of Invention
The invention aims to provide a microbial microsphere carrier for treating various environmental pollutants and a preparation method thereof, so as to solve the problem that the existing suspended microbial carrier filler for treating the pollutants needs to be improved due to risks and reasons of relatively high price, non-environment-friendly raw materials and the like in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
the microbial microsphere carrier for treating various environmental pollutants comprises the following raw materials: 18 parts of raw material powder, 1 part of microbial preparation I by volume and 1 part of preparation II by mass.
Further, the raw material powder is prepared by grinding attapulgite to 42 to 53 mesh.
Furthermore, if attapulgite powder is used as raw material powder, a reinforcing agent accounting for 0-1 percent of the total mass of the raw materials of the microsphere carrier is also required to be added.
Further, the preparation method of the reinforcing agent comprises the following steps: and uniformly mixing the solid powdery calcium oxide and the silicon dioxide according to the proportion of 3 to 1.
Further, the first microbial preparation is a microbial flora comprising any one or more of pseudomonas, lactobacillus, bacillus, yeast and photosynthetic bacteria.
Further, the preparation II comprises the following raw materials: 1000 parts by mass of wheat bran, 1 part by volume of molasses and 4 parts by volume of microbial preparation I.
Further, the preparation method of the preparation II comprises the following steps: mixing 000 parts by mass of wheat bran, 1 part by volume of molasses and 4 parts by volume of microbial preparation I uniformly, and fermenting for 2 weeks at the temperature of 20-32 ℃.
Uniformly mixing raw materials of a microsphere carrier to obtain a microsphere carrier mixture, dissolving 5 parts by mass of the microsphere carrier mixture in 25 parts by volume of deionized water, stirring at the temperature of 21-26 ℃ for 1 hour at the speed of 80-220rpm to obtain an initial mixture, measuring by using a pH meter, and adjusting the pH of the initial mixture to be neutral by using a 1M citric acid solution; and preparing the mixture with the well-mixed pH into round granules with the diameter of 2-5 mm by using a non-heating granulator, and culturing for 1 week at the temperature of 30 ℃ to obtain the microsphere carrier.
Further, the pH of the 1M citric acid solution is 4.2.
Compared with the prior art, the invention has the beneficial effects that: the invention uses common soil as a raw material to construct a microsphere carrier capable of loading microorganisms. Meanwhile, in order to solve the problem that the microsphere carrier is easy to decompose in water flow, a reinforcing agent is added in the construction process of the microsphere carrier, so that the strength of the microsphere carrier is greatly increased. Tests prove that the enhanced microsphere carrier is not suitable for being decomposed in water, and the treatment efficiency of pollutants in sewage and sludge is greatly enhanced after the microorganisms are loaded. The microorganism loaded microsphere carrier has high biocompatibility, wide raw material sources, low cost and environmental friendliness, can be applied to various scenes such as sewage treatment, sludge treatment and treatment, soil remediation and the like, and has wide application prospect. The microorganism-loaded microsphere carrier is also used for treating the remediation of the pesticide factory wastewater, sludge and petroleum-polluted farmland soil, and has a remarkable treatment effect.
Drawings
FIG. 1 is a schematic diagram of the testing of the microsphere carrier of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the microbial microsphere carrier for treating various environmental pollutants comprises the following raw materials: 9000g of raw material powder, 500ml of microbial preparation I and 500g of microbial preparation II.
The raw material powder was prepared by grinding attapulgite to 42 mesh.
If the attapulgite powder is used as raw material powder, reinforcing agent accounting for 1 percent of the total mass of the raw materials of the microsphere carrier is also needed to be added.
The preparation method of the reinforcing agent comprises the following steps: and uniformly mixing the solid powdery calcium oxide and the silicon dioxide according to the proportion of 3 to 1.
The microbial preparation I is microbial flora comprising pseudomonas, lactobacillus, bacillus, saccharomycete and photosynthetic bacteria.
The second preparation comprises the following raw materials: 10000g of wheat bran, 10ml of molasses and 40ml of microbial preparation I.
The preparation method of the preparation II comprises the following steps: 10000g of wheat bran, 10ml of molasses and 40ml of microbial preparation I are mixed uniformly and fermented for 2 weeks at the temperature of 20 ℃.
Example 2:
the microbial microsphere carrier for treating various environmental pollutants comprises the following raw materials: 9000g of raw material powder, 500ml of microbial preparation I and 500g of microbial preparation II.
The raw material powder was prepared by grinding attapulgite to 53 mesh.
If the attapulgite powder is used as raw material powder, reinforcing agent accounting for 1 percent of the total mass of the raw materials of the microsphere carrier is also needed to be added.
The preparation method of the reinforcing agent comprises the following steps: and uniformly mixing the solid powdery calcium oxide and the silicon dioxide according to the proportion of 3 to 1.
The microbial preparation I is microbial flora comprising pseudomonas, lactobacillus, bacillus, saccharomycete and photosynthetic bacteria.
The second preparation comprises the following raw materials: 10000g of wheat bran, 10ml of molasses and 40ml of microbial preparation I.
The preparation method of the preparation II comprises the following steps: 10000g of wheat bran, 10ml of molasses and 40ml of microbial preparation I are mixed uniformly and fermented for 2 weeks at the temperature of 32 ℃.
Example 3:
the microbial microsphere carrier for treating various environmental pollutants comprises the following raw materials: 9000g of raw material powder, 500ml of microbial preparation I and 500g of microbial preparation II.
The raw material powder was prepared by grinding attapulgite to 50 mesh.
If the attapulgite powder is used as raw material powder, reinforcing agent accounting for 1 percent of the total mass of the raw materials of the microsphere carrier is also needed to be added.
The preparation method of the reinforcing agent comprises the following steps: and uniformly mixing the solid powdery calcium oxide and the silicon dioxide according to the proportion of 3 to 1.
The microbial preparation I is microbial flora comprising pseudomonas, lactobacillus, bacillus, saccharomycete and photosynthetic bacteria.
The second preparation comprises the following raw materials: 10000g of wheat bran, 10ml of molasses and 40ml of microbial preparation I.
The preparation method of the preparation II comprises the following steps: 10000g of wheat bran, 10ml of molasses and 40ml of microbial preparation I are uniformly mixed and fermented for 2 weeks at the temperature of 30 ℃.
Example 4:
the microbial microsphere carrier for treating various environmental pollutants comprises the following raw materials: 9000g of raw material powder, 500ml of microbial preparation I and 500g of microbial preparation II.
The raw material powder was prepared by grinding attapulgite to 50 mesh.
The preparation method of the reinforcing agent comprises the following steps: and uniformly mixing the solid powdery calcium oxide and the silicon dioxide according to the proportion of 3 to 1.
The microbial preparation I is microbial flora comprising pseudomonas, lactobacillus, bacillus, saccharomycete and photosynthetic bacteria.
The second preparation comprises the following raw materials: 10000g of wheat bran, 10ml of molasses and 40ml of microbial preparation I.
The preparation method of the preparation II comprises the following steps: 10000g of wheat bran, 10ml of molasses and 40ml of microbial preparation I are uniformly mixed and fermented for 2 weeks at the temperature of 30 ℃.
Example 5:
the microbial microsphere carrier for treating various environmental pollutants comprises the following raw materials: 9000g of raw material powder, 500ml of microbial preparation I and 500g of microbial preparation II.
The raw material powder was prepared by grinding attapulgite to 50 mesh.
If the attapulgite powder is used as raw material powder, a reinforcing agent accounting for 0.75 percent of the total mass of the raw materials of the microsphere carrier is also required to be added.
The preparation method of the reinforcing agent comprises the following steps: and uniformly mixing the solid powdery calcium oxide and the silicon dioxide according to the proportion of 3 to 1.
The microbial preparation I is microbial flora comprising pseudomonas, lactobacillus, bacillus, saccharomycete and photosynthetic bacteria.
The second preparation comprises the following raw materials: 10000g of wheat bran, 10ml of molasses and 40ml of microbial preparation I.
The preparation method of the preparation II comprises the following steps: 10000g of wheat bran, 10ml of molasses and 40ml of microbial preparation I are uniformly mixed and fermented for 2 weeks at the temperature of 30 ℃.
Example 6:
9000g of raw material powder, 500ml of microbial preparation I and 500g of microbial preparation II are uniformly mixed to obtain a microsphere carrier mixture, 5g of the microsphere carrier mixture is dissolved in 25ml of deionized water, the mixture is stirred at the speed of 80rpm at the temperature of 21: DEG for 1 hour to obtain an initial mixture, and the pH of the initial mixture is measured by using a pH meter and is adjusted to be neutral by using 1M citric acid solution (pH4.2); and preparing the mixture with the well-mixed pH into round granules with the diameter of 2-5 mm by using a non-heating granulator, and culturing for 1 week at the temperature of 30 ℃ to obtain the microsphere carrier.
Example 7:
9000g of raw material powder, 500ml of microbial preparation I and 500g of microbial preparation II are uniformly mixed to obtain a microsphere carrier mixture, 5g of the microsphere carrier mixture is taken and dissolved in 25ml of deionized water, the mixture is stirred at the temperature of 26 ℃ and the speed of 220rpm for 1 hour to obtain an initial mixture, and the pH of the initial mixture is measured by using a pH meter and is adjusted to be neutral by using 1M citric acid solution (pH 4.2); and preparing the mixture with the well-mixed pH into round granules with the diameter of 2-5 mm by using a non-heating granulator, and culturing for 1 week at the temperature of 30 ℃ to obtain the microsphere carrier.
Example 8:
the preparation method of the microbial microsphere carrier for treating various environmental pollutants comprises the steps of obtaining a microsphere carrier mixture after the raw materials in the embodiment 3 are uniform, taking 5g of the microsphere carrier mixture, dissolving the microsphere carrier mixture in 25ml of deionized water, stirring the mixture at the temperature of 28 ℃ for 1 hour at the speed of 200rpm to obtain an initial mixture, measuring by using a pH meter, and adjusting the pH of the initial mixture to be neutral by using a 1M citric acid solution (pH 4.2); and preparing the mixture with the well-mixed pH into round granules with the diameter of 2-5 mm by using a non-heating granulator, and culturing for 1 week at the temperature of 30 ℃ to obtain the microsphere carrier.
Example 9:
the preparation method of the microbial microsphere carrier for treating various environmental pollutants comprises the steps of obtaining a microsphere carrier mixture after the raw materials in the embodiment 4 are uniform, taking 5g of the microsphere carrier mixture, dissolving the microsphere carrier mixture in 25ml of deionized water, stirring the mixture at the temperature of 28 ℃ for 1 hour at the speed of 200rpm to obtain an initial mixture, measuring by using a pH meter, and adjusting the pH of the initial mixture to be neutral by using a 1M citric acid solution (pH 4.2); and preparing the mixture with the well-mixed pH into round granules with the diameter of 2-5 mm by using a non-heating granulator, and culturing for 1 week at the temperature of 30 ℃ to obtain the microsphere carrier.
Example 10:
the preparation method of the microbial microsphere carrier for treating various environmental pollutants comprises the steps of obtaining a microsphere carrier mixture after the raw materials in the embodiment 5 are uniform, taking 5g of the microsphere carrier mixture, dissolving the microsphere carrier mixture in 25ml of deionized water, stirring the mixture at the temperature of 28 ℃ for 1 hour at the speed of 200rpm to obtain an initial mixture, measuring by using a pH meter, and adjusting the pH of the initial mixture to be neutral by using a 1M citric acid solution (pH 4.2); and preparing the mixture with the well-mixed pH into round granules with the diameter of 2-5 mm by using a non-heating granulator, and culturing for 1 week at the temperature of 30 ℃ to obtain the microsphere carrier.
The microbial flora is screened from solid wastes such as sewage outlet sludge of pesticide plants, oil extraction sludge, domestic garbage landfill sludge and the like by a technical team of the company. The biological flora contains 12 strains of pseudomonas, lactobacillus, bacillus, saccharomycetes, photosynthetic bacteria and the like, and can convert pollutants into carbon dioxide and water without secondary pollution by taking polycyclic aromatic hydrocarbon, petroleum hydrocarbon and hydrocarbon compounds as main nutritional ingredients.
Microsphere carrier assay
The experiment adopts the microsphere carrier prepared in example 6, and the experimental process comprises the following steps: a jet pump is placed at the lower part of a 50-liter water tank, sewage (a sample is taken from a sewage river) is poured into the tank, and microsphere carriers are scattered, so that water flow and the microsphere carriers form circulation in the water, and the flow rate is adjusted to be 3-5 liters per minute. And observing the properties of the microspheres, and taking a water sample to detect the sewage index.
The above experiment was repeated three times and the data are mean values.
Testing the property of the microsphere carrier: through the test of a hardness detector, the hardness of the microsphere carrier added with 1% of the reinforcing agent is 6-7 times higher than that of the microsphere carrier without the reinforcing agent, and the pH value is 8.3-8.5; the hardness of the microsphere carrier added with 0.75 percent of reinforcing agent is 4 to 5 times higher than that of the microsphere carrier without the reinforcing agent, and the pH value is 7.2 to 7.4. Therefore, when the addition amount of the reinforcing agent is 0.75%, the pH value of the microsphere carrier does not need to be additionally adjusted, and the preparation is simpler.
And testing the strength of the microsphere carrier in a flowing state. The experimental result shows that the microsphere carrier without the reinforcing agent is gradually decomposed from the beginning of 24 hours, and the microsphere carrier is completely decomposed after 72 hours; the microsphere carrier with 0.75% of the reinforcing agent was stable after 72 hours and slightly disintegrated after 7 days. This result demonstrates that the microsphere carrier with 0.75% of the reinforcing agent can be used in flowing water bodies such as sewage treatment plants or rivers.
After 15 days of reaction time, each group of water samples was sampled and analyzed by a water quality detector, and the data are shown in table 3.1.
TABLE 3.1 Water quality analysis of various groups of Water samples
Data analysis shows that if the reinforcing agent is not added into the microsphere carrier, the treatment effect on pollutants in water is not outstanding; after the microsphere carrier added with 0.75 percent of reinforcing agent is used for treatment, the water quality is obviously improved. Therefore, the carrier plays an extremely important role in repairing the water body by microorganisms.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (9)
1. A microbial microsphere carrier for treating various environmental pollutants, which is characterized in that: the method comprises the following raw materials: 18 parts of raw material powder, 1 part of microbial preparation I by volume and 1 part of preparation II by mass.
2. The microbial microsphere carrier for the treatment of various environmental pollutants according to claim 1, wherein: the raw material powder was prepared by grinding attapulgite to 42 to 53 mesh.
3. The microbial microsphere carrier for the treatment of various environmental pollutants according to claim 2, wherein: if the attapulgite powder is used as raw material powder, a reinforcing agent accounting for 0-1 percent of the total mass of the raw materials of the microsphere carrier is also required to be added.
4. The microbial microsphere carrier for the treatment of various environmental pollutants according to claim 3, wherein: the preparation method of the reinforcing agent comprises the following steps: and uniformly mixing the solid powdery calcium oxide and the silicon dioxide according to the proportion of 3 to 1.
5. The microbial microsphere carrier for the treatment of various environmental pollutants according to claim 1, wherein: the microbial preparation I is microbial flora, and comprises one or more of pseudomonas, lactobacillus, bacillus, yeast and photosynthetic bacteria.
6. The microbial microsphere carrier for the treatment of various environmental pollutants according to claim 1, wherein: the second preparation comprises the following raw materials: 1000 parts by mass of wheat bran, 1 part by volume of molasses and 4 parts by volume of microbial preparation I.
7. The microbial microsphere carrier for the treatment of various environmental pollutants according to claim 6, wherein: the preparation method of the preparation II comprises the following steps: mixing 000 parts by mass of wheat bran, 1 part by volume of molasses and 4 parts by volume of microbial preparation I uniformly, and fermenting for 2 weeks at the temperature of 20-32 ℃.
8. The preparation method of the microbial microsphere carrier for treating various environmental pollutants is characterized by comprising the following steps of: uniformly mixing raw materials of a microsphere carrier to obtain a microsphere carrier mixture, dissolving 5 parts by mass of the microsphere carrier mixture in 25 parts by volume of deionized water, stirring at the temperature of 21-26 ℃ for 1 hour at the speed of 80-220rpm to obtain an initial mixture, measuring by using a pH meter, and adjusting the pH of the initial mixture to be neutral by using a 1M citric acid solution; and preparing the mixture with the well-mixed pH into round granules with the diameter of 2-5 mm by using a non-heating granulator, and culturing for 1 week at the temperature of 30 ℃ to obtain the microsphere carrier.
9. The microbial microsphere carrier for the treatment of various environmental pollutants according to claim 1, wherein: the pH of the 1M citric acid solution was 4.2.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3993498A (en) * | 1974-04-08 | 1976-11-23 | Cullinan Holdings Limited | Granular carrier material |
| CN103667152A (en) * | 2013-12-19 | 2014-03-26 | 云南晋企生物科技有限公司 | Universal microorganism bacterial agent |
| CN104876675A (en) * | 2015-04-02 | 2015-09-02 | 新疆友合生物科技有限公司 | Organic fertilizer with attapulgite as microbial carrier, and preparation method thereof |
| CN105462880A (en) * | 2015-12-10 | 2016-04-06 | 南平正大欧瑞信生物科技开发有限公司 | Preparation method of sewage processing complex bacterium |
-
2019
- 2019-11-23 CN CN201911159886.3A patent/CN110982809A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3993498A (en) * | 1974-04-08 | 1976-11-23 | Cullinan Holdings Limited | Granular carrier material |
| CN103667152A (en) * | 2013-12-19 | 2014-03-26 | 云南晋企生物科技有限公司 | Universal microorganism bacterial agent |
| CN104876675A (en) * | 2015-04-02 | 2015-09-02 | 新疆友合生物科技有限公司 | Organic fertilizer with attapulgite as microbial carrier, and preparation method thereof |
| CN105462880A (en) * | 2015-12-10 | 2016-04-06 | 南平正大欧瑞信生物科技开发有限公司 | Preparation method of sewage processing complex bacterium |
Non-Patent Citations (1)
| Title |
|---|
| 王辉: "凹凸棒石微生物固定化载体的研制", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
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