CN110982705A - Fermentation process of schizochytrium limacinum - Google Patents
Fermentation process of schizochytrium limacinum Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 79
- 230000004151 fermentation Effects 0.000 title claims abstract description 79
- 241000003595 Aurantiochytrium limacinum Species 0.000 title claims abstract description 37
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000001963 growth medium Substances 0.000 claims abstract description 22
- 239000000843 powder Substances 0.000 claims abstract description 22
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 18
- 241000233671 Schizochytrium Species 0.000 claims abstract description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 10
- 241000195493 Cryptophyta Species 0.000 claims abstract description 8
- 238000011081 inoculation Methods 0.000 claims abstract description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 16
- 239000008103 glucose Substances 0.000 claims description 16
- 239000007787 solid Substances 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 9
- 229940041514 candida albicans extract Drugs 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 238000011218 seed culture Methods 0.000 claims description 9
- 235000002639 sodium chloride Nutrition 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- 239000012138 yeast extract Substances 0.000 claims description 9
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 239000002609 medium Substances 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- 241000047245 Schizochytrium sp. SLTW3 Species 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 4
- 230000001133 acceleration Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 239000001103 potassium chloride Substances 0.000 claims description 3
- 235000011164 potassium chloride Nutrition 0.000 claims description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 3
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 3
- 229910021529 ammonia Inorganic materials 0.000 claims description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- 239000002028 Biomass Substances 0.000 abstract description 10
- 235000014113 dietary fatty acids Nutrition 0.000 abstract description 7
- 239000000194 fatty acid Substances 0.000 abstract description 7
- 229930195729 fatty acid Natural products 0.000 abstract description 7
- 150000004665 fatty acids Chemical class 0.000 abstract description 7
- 238000009360 aquaculture Methods 0.000 abstract description 6
- 244000144974 aquaculture Species 0.000 abstract description 6
- 239000002994 raw material Substances 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000009776 industrial production Methods 0.000 abstract description 3
- 239000000306 component Substances 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 4
- 235000019198 oils Nutrition 0.000 description 4
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 235000011114 ammonium hydroxide Nutrition 0.000 description 3
- 238000005265 energy consumption Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 241000598397 Schizochytrium sp. Species 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000021323 fish oil Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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Abstract
The invention provides a fermentation process of schizochytrium limacinum, which comprises the following steps: (1) taking schizochytrium limacinum algae seeds, and preparing a seed solution; (2) inoculating the seed solution prepared in the step (1) into a culture medium, wherein the inoculation amount of the seed solution is 2-3%; (3) fermenting at 25-28 deg.C for 48-56 h. The schizochytrium limacinum algae powder prepared by the fermentation process has the protein content of 45-50%, the fatty acid content of 25-35% and the DHA content of 10-15%, and is more suitable for aquaculture compared with the algae powder in the prior art. In addition, the fermentation process can obtain higher schizochytrium biomass and DHA content in shorter fermentation time by controlling the feeding time of the carbon source and the nitrogen source. Meanwhile, the fermentation process greatly reduces the cost of the nitrogen source in the raw materials and the total fermentation cost. In addition, the process of feeding the carbon source and the nitrogen source is easy to operate, is suitable for large-scale fermentation of schizochytrium limacinum, and is beneficial to industrial production.
Description
Technical Field
The invention relates to a fermentation process of schizochytrium limacinum.
Background
Schizotocytum limacinum is a marine fungus, and has the characteristics of rapid growth and high oil content, wherein more than 90% of total oil is unsaturated fatty acid DHA. The DHA in the schizochytrium limacinum powder is completely consistent with the DHA in the fish oil in form, and the EPA content is lower, so that the schizochytrium limacinum powder is easier to be absorbed by a human body.
Schizochytrium limacinum is one of the main strains for industrially producing DHA at present, and a plurality of patents on the aspect of utilizing schizochytrium limacinum for fermentation exist, and relate to the fields of strain improvement, culture medium selection, fermentation process optimization and the like. At present, CN106636235, CN104031843 and CN105018539 are main patents on schizochytrium fermentation process research for obtaining high-yield biomass and DHA, wherein the CN106636235 patent adopts a split tank fermentation method to ensure that the biomass of the schizochytrium reaches 120-180g/L, the oil content reaches 45-60 percent, and the DHA accounts for 40-50 percent of the oil content; although the method of split-tank fermentation can obtain higher schizochytrium biomass and grease, the split-tank fermentation also has some disadvantages, such as longer fermentation time, difficult operation in actual production and higher risk of bacterial contamination in the fermentation process. The low-temperature stress fermentation process disclosed in CN105018539 can enable the biomass of schizochytrium limacinum to reach 150-one (120-150 g/L) and DHA to reach 40-50g/L, and the fermentation process also has the defects of long fermentation time and energy consumption increase caused by low-temperature fermentation. CN104031843 discloses a fermentation method of schizochytrium limacinum, wherein the biomass of the schizochytrium limacinum is 180-200g/L, and the DHA yield is 50-65g/L, which is a fermentation process reporting that the maximum biomass and DHA content of the schizochytrium limacinum are achieved by fermentation.
At present, the nutrient indexes of the algae powder obtained by fermenting schizochytrium limacinum in China are generally 20-25% of protein content, 50-60% of fatty acid content and 25-30% of DHA content; the content of the protein, the content of the fatty acid and the content of the DHA of the schizochytrium limacinum powder which is suitable for aquaculture is 45-50%, 25-35% and 10-15%. Therefore, the nutrient structure components of the algae powder obtained by fermenting schizochytrium in the prior art are not suitable for aquaculture. Therefore, there is a need to research the schizochytrium algae powder suitable for aquaculture.
Disclosure of Invention
In order to solve the problems, the invention provides a fermentation process of schizochytrium limacinum.
The invention provides schizochytrium limacinum which is a preservation number preserved by China center for type culture Collection: schizochytrium sp.SLTW3 with CCTCC No. M2015591. The preservation date of the schizochytrium limacinum is as follows: in 2015, 08 at 10 months, the preservation addresses are: wuhan university in Wuhan, China.
The invention also provides a fermentation process of schizochytrium limacinum, which comprises the following steps:
(1) taking schizochytrium limacinum algae seeds, and preparing a seed solution;
(2) inoculating the seed solution prepared in the step (1) into a culture medium, wherein the inoculation amount of the seed solution is 2-3%;
(3) fermenting at 25-28 deg.C for 48-56 h.
Further, in the step (1), the schizochytrium algae species is deposited by China center for type culture Collection under the accession number: schizochytrium sp.SLTW3 with CCTCC No. M2015591.
Further, in the step (1), the seed liquid is prepared by the following method: scribing schizochytrium limacinum in a solid culture medium, and culturing at 25-28 deg.C for 36-48h to obtain single colony; inoculating single colony in the seed culture medium, and performing shaking culture at 28 deg.C and 180r/min for 16-24 hr;
preferably, the inoculating single colony inoculates 1-2 single colonies per 50mL of seed medium.
Further, the components in the solid medium are as follows: 5g/L of glucose, 1g/L of yeast extract powder, 1g/L of peptone, 1g/L of sea salt and 17g/L of agar; the pH of the solid medium is 6.0;
the seed culture medium comprises the following components: 20-30g/L of glucose, 5-10g/L of yeast extract powder, 5-10g/L of peptone and 15-25g/L of sea salt; the pH of the seed culture medium is 6.0-7.0.
Further, in the step (2), the components in the culture medium are as follows: 60-90g/L of glucose, 10-20g/L of corn steep liquor, 5-10g/L of yeast extract powder, 5-10g/L of peptone, 15-25g/L of sea salt, 2-3g/L of magnesium sulfate, 2-3g/L of monopotassium phosphate, 0.5-1.5g/L of potassium chloride and 1-3g/L of ammonium sulfate;
and/or, in the step (3), the pH value during the fermentation is 6.0-7.0;
preferably, in step (3), the pH is adjusted using ammonia.
Further, in the step (3), the dissolved oxygen is 20-30% before the fermentation is carried out for 24 hours, and the dissolved oxygen is 0-5% after the fermentation is carried out for 24 hours and 24 hours;
and/or, in the step (3), feeding a nitrogen source and a carbon source during the fermentation culture for 24-48 h.
Further, the nitrogen source flow acceleration rate is 62 g/h; the carbon source flow acceleration rate is 78 g/L;
preferably, the nitrogen source is corn steep liquor and the carbon source is glucose.
Further, in the step (3), the fermentation is carried out in a fermentation tank, and the ventilation volume of the fermentation tank is 0.7-1m3The tank pressure is 0.05MPa, and the rotating speed of the fermentation tank is 200-500 rpm.
Further, in the step (3), during fermentation, the glucose content is 10-20g/L when the fermentation lasts for 24-48 h.
The content of the protein of the schizochytrium limacinum powder prepared by the fermentation process of the schizochytrium limacinum is 45-50%, the content of fatty acid is 25-35%, and the content of DHA is 10-15%, so that the schizochytrium limacinum powder is more suitable for aquaculture compared with the schizochytrium limacinum powder in the prior art (the content of the protein of the schizochytrium limacinum powder in the prior art is 20-25%, the content of fatty acid is 50-60%, and the content of DHA is 25-30%). In addition, the fermentation process can obtain higher schizochytrium biomass and DHA content in shorter fermentation time by controlling the feeding time of the carbon source and the nitrogen source. Meanwhile, the fermentation process of the invention has low energy consumption, does not need to add phosphoric acid to adjust the pH, takes ammonia water with lower cost as a pH regulator and an inorganic nitrogen source, has simple culture medium components, and greatly reduces the cost of the nitrogen source in the raw materials and the total fermentation cost. In addition, the process of feeding the carbon source and the nitrogen source is easy to operate, is suitable for large-scale fermentation of schizochytrium limacinum, and is beneficial to industrial production.
Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Detailed Description
The raw materials and equipment used in the embodiment of the present invention are known products and obtained by purchasing commercially available products.
Example 1 fermentation Process of Schizochytrium sp
1. Reviving and preparing schizochytrium limacinum seed liquid
The Schizochytrium sp (Schizochytrium sp. SLTW 3. of CCTCC No: M2015591) preserved in a glycerol tank at-80 deg.C is streaked and cultured on a plate filled with a solid culture medium, and the single colony is obtained by culturing in a solid culture box at 25-28 deg.C for 36-48 h.
Inoculating 1-2 single colonies into a 250mL triangular flask containing 50mL seed culture medium, and performing shake culture at 28 deg.C and 180r/min for 16-24h to obtain seed solution.
The solid medium had the following composition (g/L): glucose 5, yeast extract powder 1, peptone 1, sea salt 1 and agar 17; the pH of the solid medium is 6.0;
the seed culture medium comprises the following components (g/L): 20-30 parts of glucose, 5-10 parts of yeast extract powder, 5-10 parts of peptone and 15-25 parts of sea salt; the pH of the seed culture medium is 6.0-7.0.
2. The invention relates to a fermentation process of schizochytrium limacinum
Inoculating 2-3% of schizochytrium limacinum seed liquid into a 20L fermentation tank filled with 12L of sterilization culture medium, and controlling the whole fermentation temperature at 25-28 ℃; and regulating the pH value of the fermentation to be 6.0-7.0 by using ammonia water in the whole process, and when the pH value is higher than 7.0, regulating and controlling are not needed.
The medium composition in the fermenter was as follows (g/L): 60-90 parts of glucose, 10-20 parts of corn steep liquor, 5-10 parts of yeast extract, 5-10 parts of peptone, 15-25 parts of sea salt, 2-3 parts of magnesium sulfate, 2-3 parts of monopotassium phosphate, 0.5-1.5 parts of potassium chloride and 1-3 parts of ammonium sulfate.
The ventilation capacity of the fermentation tank is 0.7-1m3H; the tank pressure is 0.05 MPa; the rotation speed is 200-500 rpm. Before 24h of fermentation, the dissolved oxygen is controlled at 20-30%, and after 24h and 24h, the dissolved oxygen is controlled at 0-5%. In the fermentation process, dissolved oxygen is used as a control index, and the ventilation volume, the tank pressure and the stirring speed are adjusted. Corn steep liquor (nitrogen source) and glucose (carbon source) are fed during 24-48h of fermentation culture, the feeding rate of the corn steep liquor is controlled to be about 62g/h, the feeding rate of the glucose is controlled to be about 78g/L, and the glucose content in a fermentation tank is kept to be 10-20g/L between 24-48 h. After 48h of fermentation, the obtained schizochytrium biomass is 140-140 g/L, the protein content is 45-50%, the total fatty acid content is 25-35%, and the DHA content is 10-15%.
In conclusion, the schizochytrium powder prepared by the fermentation process of the schizochytrium has the protein content of 45-50%, the fatty acid content of 25-35% and the DHA content of 10-15%, and is more suitable for aquaculture compared with the schizochytrium powder in the prior art. In addition, the fermentation process can obtain higher schizochytrium biomass and DHA content in shorter fermentation time by controlling the flow and the adding time of the carbon source and the nitrogen source. Meanwhile, the fermentation process of the invention has low energy consumption, does not need to add phosphoric acid to adjust the pH, takes ammonia water with lower cost as a pH regulator and an inorganic nitrogen source, has simple culture medium components, and greatly reduces the nitrogen source cost in the raw materials and the total fermentation cost. In addition, the process of feeding the carbon source and the nitrogen source is easy to operate, is suitable for large-scale fermentation of schizochytrium limacinum, and is beneficial to industrial production.
Claims (10)
1. A schizochytrium limacinum, characterized in that: it is a preservation number preserved by China center for type culture Collection: schizochytrium sp.SLTW3 of M2015591, CCTCC No.
2. A fermentation process of schizochytrium limacinum is characterized in that: the method comprises the following steps:
(1) taking schizochytrium limacinum algae seeds, and preparing a seed solution;
(2) inoculating the seed solution prepared in the step (1) into a culture medium, wherein the inoculation amount of the seed solution is 2-3%;
(3) fermenting at 25-28 deg.C for 48-56 h.
3. The fermentation process of claim 2, wherein: in the step (1), the schizochytrium algae species is preserved by China center for type culture Collection with a preservation number: schizochytrium sp.SLTW3 with CCTCC No. M2015591.
4. The fermentation process of claim 2, wherein: in the step (1), the seed liquid is prepared by the following method: streaking schizochytrium limacinum in a solid culture medium, and culturing at 25-28 deg.C for 36-48h to obtain single colony; inoculating single colony in the seed culture medium, and performing shaking culture at 28 deg.C and 180r/min for 16-24 hr;
preferably, the inoculating single colony inoculates 1-2 single colonies per 50mL of seed medium.
5. The fermentation process of claim 4, wherein: the solid culture medium comprises the following components: 5g/L of glucose, 1g/L of yeast extract powder, 1g/L of peptone, 1g/L of sea salt and 17g/L of agar; the pH of the solid medium is 6.0;
the seed culture medium comprises the following components: 20-30g/L of glucose, 5-10g/L of yeast extract powder, 5-10g/L of peptone and 15-25g/L of sea salt; the pH of the seed culture medium is 6.0-7.0.
6. The fermentation process of claim 2, wherein: in the step (2), the components in the culture medium are as follows: 60-90g/L of glucose, 10-20g/L of corn steep liquor, 5-10g/L of yeast extract powder, 5-10g/L of peptone, 15-25g/L of sea salt, 2-3g/L of magnesium sulfate, 2-3g/L of monopotassium phosphate, 0.5-1.5g/L of potassium chloride and 1-3g/L of ammonium sulfate;
and/or, in the step (3), the pH value during the fermentation is 6.0-7.0;
preferably, in step (3), the pH is adjusted using ammonia.
7. The fermentation process of claim 2, wherein: in the step (3), the dissolved oxygen is 20-30% before fermentation for 24 hours, and the dissolved oxygen is 0-5% after fermentation for 24 hours and 24 hours;
and/or, in the step (3), feeding a nitrogen source and a carbon source during the fermentation culture for 24-48 h.
8. The fermentation process of claim 7, wherein: the nitrogen source flow acceleration rate is 62 g/h; the carbon source flow acceleration rate is 78 g/L;
preferably, the nitrogen source is corn steep liquor and the carbon source is glucose.
9. The fermentation process of claim 2, wherein: in the step (3), the fermentation is carried out in a fermentation tank, and the ventilation volume of the fermentation tank is 0.7-1m3The tank pressure is 0.05MPa, and the rotating speed of the fermentation tank is 200-500 rpm.
10. The fermentation process of claim 2, wherein: in the step (3), in the fermentation, the glucose content is 10-20g/L when the fermentation is carried out for 24-48 h.
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| CN111303988A (en) * | 2020-04-17 | 2020-06-19 | 珀莱雅化妆品股份有限公司 | Preparation method and application of sterol-rich algae oil |
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| CN111303988A (en) * | 2020-04-17 | 2020-06-19 | 珀莱雅化妆品股份有限公司 | Preparation method and application of sterol-rich algae oil |
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