CN110982697A

CN110982697A - Cell culture device for biotechnology research and development

Info

Publication number: CN110982697A
Application number: CN202010137570.0A
Authority: CN
Inventors: 于淑莉
Original assignee: Guangzhou Zagene Technology Co ltd
Current assignee: Guangzhou Zagene Technology Co ltd
Priority date: 2020-03-03
Filing date: 2020-03-03
Publication date: 2020-04-10
Anticipated expiration: 2040-03-03
Also published as: CN110982697B

Abstract

The invention discloses a cell culture device for biotechnology research and development, which comprises an incubator, frame plates, an air exchange module and a sterilization filter liquid box, wherein a plurality of layers of frame plates are arranged at the upper part in the incubator and used for placing cell culture dishes, the sterilization filter liquid box is arranged at the bottom of the incubator, the air exchange module is arranged on the outer surface of the side surface of the incubator, fresh air is introduced into the air exchange module from the outside and enters the sterilization filter liquid box, the air exchange module also absorbs internal gas from the top in the incubator and exhausts the internal gas to the outside, and sterilization liquid is injected into the sterilization filter liquid box. The air exchange module comprises a first filter screen, a second filter screen, a first fan, a second fan, an air inlet pipe, a fresh air pipe, an air return pipe, an exhaust pipe and a path change valve group, wherein the path change valve group is provided with four interfaces which are respectively connected with the air inlet pipe, the exhaust pipe, the first filter screen and the second filter screen; the first filter screen and the second filter screen are alternately used for filtering, and the backflow gas cleaning filter screen keeps long-term high efficiency.

Description

Cell culture device for biotechnology research and development

Technical Field

The invention relates to the field of cell culture, in particular to a cell culture device for biotechnology research and development.

Background

Cell culture is an important and widely applied technology in the field of biotechnology, and many scientific experiments and researches require appropriate cell culture.

In the process of cell culture, cells also have metabolism, metabolites are discharged in time and affect the growth environment of the cells, many gas environments for cell culture use air components, but the operation of dust removal and sterilization is needed, otherwise, dust and bacteria fall on cell strains and affect the growth of the cells, so that how to perform efficient air replacement, sterilization and dust removal of the cell incubator is the design key point of the cell culture device, in addition, many cell incubators are very large, and because the growth environment of the cells generally needs to be 'greenhouse conditions' higher than the air temperature, if the gas in the incubator is directly discharged out of the device in the ventilation process, a large amount of heat is lost, a high-power heater is needed to be additionally used for heating operation of fresh clean air, and the energy utilization is low.

Disclosure of Invention

The invention aims to provide a cell culture device for biotechnology research, which solves the problems in the prior art.

In order to achieve the purpose, the invention provides the following technical scheme:

the utility model provides a cell breeding device is used in biotechnology research, includes incubator, frame plate, the module of taking a breath and the filtrate case that disinfects, and upper portion is equipped with a plurality of layers of frame plate and is used for placing the cell culture ware in the incubator, and incubator bottom sets up the filtrate case that disinfects, and the module of taking a breath sets up the side outward appearance at the incubator, and the module of taking a breath introduces fresh air from the external world and gets into the filtrate case that disinfects, and the module of taking a breath still absorbs inside gas from the top in the incubator and arranges to the external world, and the filtrate incasement that disinfects is annotated.

The cell culture dish placed in the incubator is a final use component of the invention, the incubator provides a culture environment for the incubator, including a gas environment, a temperature environment and the like, the ventilation module sucks external air into the module and then discharges the air into the sterilization filtrate box, the air is filtered and sterilized by solution in the sterilization filtrate box and then enters the incubator, the original air in the incubator is discharged out of the incubator from the top and is discharged out of the device through the ventilation module, so that the stable establishment of the gas environment is completed, a sterile environment which is consistent with the content of external gas components is maintained, and the normal growth of cells is ensured.

Furthermore, the air exchange module comprises a first filter screen, a second filter screen, a first fan, a second fan, an air inlet pipe, a fresh air pipe, an air return pipe, an exhaust pipe and a path change valve group, wherein the path change valve group is provided with four interfaces which are respectively connected with the air inlet pipe, the exhaust pipe, the first filter screen and the second filter screen;

the other side of the first filter screen is connected with a first fan, the other end of the second filter screen is connected with a second fan, the other end of the first fan is divided into two branches which are respectively connected with a fresh air pipe and a return air pipe, the other end of the second fan is also divided into two branches which are respectively connected with the fresh air pipe and the return air pipe, one end of the fresh air pipe, which is far away from the first fan, is a fresh air inlet, one end of the return air pipe, which is far away from the first fan, is a return air inlet, the fresh air inlet extends into the sterilization filtrate tank, the return air inlet is positioned at the upper part of the incubator, one-way valves facing the fresh air inlet are arranged on the two branches of the fresh air pipe, which are respectively connected with the first fan and the second fan, and one-way valves facing;

one end of the air inlet pipe, which is far away from the route changing valve group, is an air inlet, the air inlet enters the route changing valve group through a pipeline and then is respectively connected with the first filter screen and the second filter screen through two branches, and check valves facing the first filter screen and the second filter screen are respectively arranged in the route changing valve group and on the two branches from the air inlet to the first filter screen and the second filter screen; the exhaust pipe is kept away from the one end of the route changing valve group and is an exhaust port, the exhaust port enters the route changing valve group through a pipeline and then is connected with the first filter screen and the second filter screen through two branch circuits respectively, and one-way valves departing from the first filter screen and the second filter screen are arranged in the route changing valve group and on the two branch circuits from the exhaust port to the first filter screen and the second filter screen respectively.

If the sterilizing filter box provides filtering operation after the outside air enters the device, the filtrate can be quickly consumed and polluted, so that the filter is frequently replaced, the sterilizing filter box mainly plays a role in sterilizing, the main filtering process is completed by the first filter screen and the second filter screen, the filtering load of the sterilizing filter box is reduced, the frequent replacement is not needed, the arrangement of the two filter screens can help the device to carry out the flushing operation of the filter screens, namely, one filter screen is used as a filtering part to carry out the filtering function, the other filter screen can be flushed by gas flowing reversely, the filter screens filter dust particles which are basically from the outside atmosphere and only exist on one side of the switching valve group connected with the first filter screen and the second filter screen, the other sides of the two filter screens are in contact with the gas from the incubator, the air in the incubator does not contain dust, the metabolites of cells and the like belong to nano-level small molecules, therefore, the dust-proof air-purifying device can easily penetrate through the filter screen to be discharged to the outside, when the first fan is used as an induced draft fan (sucking air from the outside and blowing the air into the incubator), the second fan is stopped or used as an air outlet fan, one end of the first fan is connected to the air inlet under the two flow paths of the two fans, the first filter screen is used as a filtering part to perform filtering function, the dust of the outside air is isolated at one side of the first filter screen connected with the road changing valve group, the outside air reaches the fresh air port from a branch on the fresh air pipe after passing through the first fan and then is discharged into the sterilizing filter box, and the dust is discharged in the sterilizing filter box in the form of bubbles, at the moment, most of the dust is isolated, and only a few of the dust which can penetrate through the first filter screen enters the sterilizing filter box and is contacted with filtrate after being discharged in the form of bubbles, the fine dust is adhered to the filtrate again for secondary filtration, and the gas is contacted with the sterilizing liquid while the secondary filtration is carried out, so that the disinfection and sterilization process is completed, and the bubbles emerging from the sterilizing filtrate tank are dust-free and sterile clean gas;

dirty gas in the incubator enters the return air pipe from the return air inlet, and high-pressure air is arranged on the outlet of the first fan, so that a branch of the return air inlet leading to the first fan is closed due to the relation of the one-way valve and can only reach one end of the second fan through one branch, then the branch passes through the second fan and is discharged to the path change valve group through the second filter screen, in the path change valve group, the air passing through the second filter screen is discharged into the exhaust pipe through the branch where the one-way valve pointing to the exhaust pipe is located, the gas discharge process is completed, and in the process, when the exhaust gas passes through the second filter screen, dust and impurities accumulated before the second filter screen can be taken away, so that the purpose of cleaning the second filter screen is achieved;

after the operation process is carried out for a period of time, the operation logic is switched, under the logic, the first fan is used as an air outlet fan, the second fan is used as an induced draft fan, at the moment, in the path changing valve group, the branch is switched, the branch with the air inlet connected with the second fan is used as an air inlet branch, the second filter screen is used as a filtering component, the branch from the first fan to the exhaust pipe is used as an air outlet branch, and the first filter screen is not used as a filtering component any more and is cleaned by air from the incubator;

the two filter screens for alternate filtration can play a role in filtration without maintenance for a long time.

Furthermore, a wire mesh plate is arranged at the upper layer of the water body in the sterilization filtrate tank, the wire mesh plate is horizontally arranged, the aperture of the wire mesh is smaller than one millimeter, and the wire mesh plate is positioned above the fresh air port.

After the gas is blown out from the fresh air port, large-size bubbles possibly exist, the large-size bubbles are not fully contacted with the filtrate, the filtering and sterilizing effects are not obvious, and after the wire mesh plate is added, the large-size bubbles reach the lower surface of the wire mesh plate, are cut into a cluster of fine bubbles by the wire mesh plate, are fully contacted with the sterilizing liquid, and the sterilizing and secondary filtering operations are completed.

Furthermore, the exhaust pipe extends from the path changing valve group, then bends to enter the sterilization filtrate tank, and then extends to be exhausted to the outside atmosphere, and a differential pressure valve is arranged on the exhaust pipe and on a section from the sterilization filtrate tank to the air outlet.

The pressure difference valve has a pipeline flow resistance, so that a section of pipeline in front of the pressure difference valve on the exhaust pipe has a pressure which is one level higher than that of an exhaust port (external atmospheric pressure), the higher pressure is approximately equal to the flow resistance of the pressure difference valve, the higher pressure needs to be pressurized by the first fan or the second fan to cause pressure rise in the front half section of the exhaust pipe, after the pressure rise, the gas temperature rises, the gas exhausted from the exhaust pipe is the air from the incubator due to the requirement, the incubator is a 'greenhouse' environment with the temperature higher than the external atmosphere, if the incubator is a large-scale incubator, if the gas heat is directly exhausted out of the incubator, a large amount of heat is wasted, and additional heat supply is needed to heat the air entering the incubator from the outside, so the heat recovery process is best performed, the heat recovery process of the invention carries out heat recovery through the exhaust pipe and the pressure difference valve, after the gas in the exhaust pipe is compressed and heated, the exhaust pipe is bent to enter the sterilization filtrate box, the temperature is transferred to the solution, and the solution heats fresh air, so that heat recovery is completed.

As optimization, one section of the exhaust pipe, which is positioned in the sterilization filtrate box, is provided with a coil pipe, and the outer surface of the coil pipe is provided with fins. The coil and the fins on the surface of the coil enhance the heat exchange effect.

Furthermore, the differential pressure valve is a self-control valve, the position on the air inlet pipe, which is close to the air inlet, and the position on the exhaust pipe, which is close to the exhaust port, are respectively connected to two ends of a temperature difference meter, the temperature difference meter detects the temperature difference between the air inlet and the exhaust port and controls the differential pressure valve, and the larger the temperature difference between the two is, the larger the flow resistance of the differential pressure valve is caused by adjusting the differential pressure valve.

One section gas before the differential pressure valve in the exhaust pipe flows and carries out the heat transfer when advancing the rose box that disinfects, and the gas temperature after the heat transfer drops slightly, then when carrying out pressure reduction, gaseous inflation in differential pressure valve rear, the temperature can reduce voluntarily, if reduce to outdoor atmospheric temperature, so whole device just can establish the balance with external heat: the continuous gas entering and exiting the incubator, therefore, the total amount of gas is not changed, then, the temperature of the inlet and outlet are consistent, and the temperature in the incubator can be kept at a stable level, wherein the heat generation caused by the heat generation and the flow resistance caused by the work of the fan is not considered, the heat is negligible compared with the incubator with a large volume and the air flow with a larger flow rate, or the heat is basically considered to be balanced with the heat dissipation of the outer surface of the device, and if the temperature is to be stabilized very accurately, a heater is also required to be added in the incubator.

Preferably, the first fan and the second fan are bidirectional axial fans. The bidirectional axial flow fans can flow in two directions, and when one fan is used as a draught fan, the other fan is used as an air outlet fan.

As optimization, a stirring blade is arranged in the sterilization filtrate tank. The solution in the sterilization filtrate tank is stirred and turned by the stirring blade and is fully contacted with fresh air for sterilization. The bubbles of fresh air can be further refined.

Compared with the prior art, the invention has the beneficial effects that: the invention carries out the ventilation operation of the incubator through the ventilation module, not only can maintain high-efficiency filtering capacity for a long time, but also can maintain stable disinfection and sterilization effects, the incubator is sucked by a first fan or a second fan as a draught fan, fresh air in a sterilization filtrate box is injected through a fresh air port, most dust is filtered when the incubator passes through a first filter screen or a second filter screen, the bubbles which are blown out are cut into a cluster of small bubbles by a stirring paddle and a wire mesh plate, the small bubbles are fully contacted with sterilization liquid for sterilization and secondary filtration, the original dirty gas is filled into the incubator to be replaced, the original gas enters a return air pipe from a return air port, the dirty gas in the return air pipe is discharged to an exhaust pipe by the first fan or the second fan as an air outlet machine, the filter screen is cleaned when the discharged gas passes through the filter screen, the exhaust pipe can operate without maintenance for a long time, and the gas in the exhaust pipe is compressed by a pressure difference valve, the temperature rises, transfers heat to the solution in the sterilization filtrate box, and the solution self expands and cools at the rear of the differential pressure valve, and the temperature of the solution keeps the same with the temperature of the outside air when entering the device when the air outlet is arranged, so that the heat of the original gas in the incubator is fully recovered, the energy is fully utilized, and the waste is avoided.

Drawings

In order that the present invention may be more readily and clearly understood, a more particular description of the invention briefly described above will be rendered by reference to specific embodiments that are illustrated in the appended drawings.

FIG. 1 is a schematic diagram of the general structure of the present invention;

FIG. 2 is a schematic external view of the present invention;

FIG. 3 is a schematic view of the ventilation module according to the present invention;

FIG. 4 is a first schematic view illustrating the operation of the ventilation module according to the present invention;

fig. 5 is a second schematic view illustrating the operation of the ventilation module according to the present invention.

In the figure: 1-incubator, 2-frame plate, 3-ventilation module, 301-air inlet, 302-fresh air inlet, 303-air return inlet, 304-air outlet, 31-first filter screen, 32-second filter screen, 33-first fan, 34-second fan, 35-air inlet pipe, 36-fresh air pipe, 37-air return pipe, 38-air exhaust pipe, 381-coil pipe, 382-differential pressure valve, 39-path-changing valve group, 4-sterilization filtrate tank and 5-wire mesh plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in fig. 1 and 2, a cell culture device for biotechnology research and development, including incubator 1, frame plate 2, module of taking a breath 3 and the filtrate case 4 that disinfects, upper portion is equipped with a plurality of layers of frame plate 2 and is used for placing the cell culture ware in incubator 1, incubator 1 bottom sets up the filtrate case 4 that disinfects, module of taking a breath 3 sets up the side outward appearance at incubator 1, module of taking a breath 3 introduces fresh air from the external world and gets into filtrate case 4 that disinfects, module of taking a breath 3 still draws inside gas from the top in incubator 1 and arranges to the external world, disinfect in the filtrate case 4 and have the liquid that disinfects.

The cell culture dish placed in the incubator 1 is a final use component of the invention, the incubator 1 provides a culture environment for the incubator, including a gas environment, a temperature environment and the like, the ventilation module 3 sucks external air into the module, then the air is discharged into the sterilization filtrate box 4, the air is filtered and sterilized by a solution in the sterilization filtrate box 4, then the air enters the incubator 1, dirty air in the incubator 1 is replaced, fresh air floats out of a liquid in the sterilization filtrate box 4 and enters an internal environment of the incubator 1, original air in the incubator 1 is discharged out of the incubator 1 from the top and is discharged out of the device through the ventilation module 3, and therefore stable establishment of the gas environment is completed, a sterile environment which is consistent with the content of external gas components is maintained, and normal growth of cells is guaranteed.

As shown in fig. 3, the air exchange module 3 includes a first filter screen 31, a second filter screen 32, a first fan 33, a second fan 34, an air inlet pipe 35, a fresh air pipe 36, an air return pipe 37, an air exhaust pipe 38, and a route change valve group 39, where the route change valve group 39 has four interfaces respectively connected to the air inlet pipe 35, the air exhaust pipe 38, the first filter screen 31, and the second filter screen 32;

the other side of the first filter screen 31 is connected with a first fan 33, the other end of the second filter screen 32 is connected with a second fan 34, the other end of the first fan 33 is divided into two branches which are respectively connected with a fresh air pipe 36 and a return air pipe 37, the other end of the second fan 34 is also divided into two branches which are respectively connected with the fresh air pipe 36 and the return air pipe 37, one end of the fresh air pipe 36 far away from the first fan 33 is a fresh air inlet 302, one end of the return air pipe 37 far away from the first fan 33 is a return air inlet 303, the fresh air inlet 302 extends into the sterilization filtrate box 4, the return air inlet 303 is positioned at the upper part in the incubator 1, one-way valves facing the fresh air inlet 302 are respectively arranged on the two branches which are respectively connected with the first fan 33 and the second fan 34 on the fresh air pipe 36, and one-way valves facing the return air inlet 303 are respectively arranged on the two branches;

one end of the air inlet pipe 35, which is far away from the route changing valve group 39, is an air inlet 301, the air inlet 301 enters the route changing valve group 39 through a pipeline and then is connected with the first filter screen 31 and the second filter screen 32 through two branches, and check valves facing the first filter screen 31 and the second filter screen 32 are arranged in the route changing valve group 39 and on two branches from the air inlet 301 to the first filter screen 31 and the second filter screen 32 respectively; the end of the exhaust pipe 38 away from the change valve group 39 is an exhaust port 304, the exhaust port 304 enters the change valve group 39 via a pipeline and then is connected with the first filter screen 31 and the second filter screen 32 through two branches, and check valves departing from the first filter screen 31 and the second filter screen 32 are arranged in the change valve group 39 and on two branches from the exhaust port 304 to the first filter screen 31 and the second filter screen 32.

If the sterilizing filter box provides a filtering operation after the outside air enters the device, the filtrate can be quickly consumed and polluted, so that the sterilizing filter box is frequently replaced, the main function of the sterilizing filter box is sterilization, the main process of the filtering is completed by the first filter screen 31 and the second filter screen 32, the filtering load of the sterilizing filter box 4 is reduced, and the frequent replacement is not needed, as shown in fig. 3, the arrangement of the two filter screens can help the device to carry out the washing operation of the filter screens, namely, one filter screen is used as a filtering part to carry out the filtering function, the other filter screen can be washed by gas flowing reversely, the filter screens filter dust particles which are basically from the outside atmosphere, only exist on one side of the first filter screen 31 and the second filter screen 32 connected with the switching valve group 39, the other sides of the two filter screens contact the gas from the incubator 1, and the air in the incubator 1 has no dust, since metabolites of cells and the like belong to nano-scale small molecules, they can easily pass through a filter screen and be discharged to the outside, and the principle of shunting in a flow path can be seen from fig. 4 and 5: as shown in fig. 4, when the first fan 33 is used as a draught fan (sucking air from the outside and blowing it into the incubator 1), the second fan 34 is either stopped or used as an air-out fan, and under the two flow paths of the two fans, one end of the first fan 31 is connected to the air inlet 301, the first filter 31 performs a filtering function as a filtering component, dust of the outside air is isolated at one side of the first filter 31 connected to the route-changing valve set 39, and after the outside air passes through the first fan 33, the outside air reaches the fresh air inlet 304 from a branch on the fresh air pipe 36, and then is discharged into the sterilizing filter box 4, and is discharged in the form of bubbles in the sterilizing filter box 4, at this time, most of the dust is isolated, and only a very small part of the dust which can pass through the first filter 31 enters the sterilizing filter box 4, and is discharged in the form of bubbles and then contacts with the filtrate, the fine dust is adhered to the filtrate again for secondary filtration, and the gas is contacted with the sterilizing liquid while the secondary filtration is carried out, so that the disinfection and sterilization process is completed, and the bubbles emitted from the sterilizing filtrate tank 4 are dust-free and sterile clean gas; the solution in the sterilizing filtrate tank 4 does not need to be replaced frequently, and the replacement period is determined according to the amount of fine dust and bacteria in the use environment and the time for which the sterilizing solution can maintain the sterilizing effect.

Dirty gas in the incubator 1 enters the return air pipe 37 from the return air inlet 303, and because high-pressure air is arranged at the outlet of the first fan 33, the branch of the return air inlet 303 leading to the first fan 33 is closed due to the relation of the one-way valve, and can only reach one end of the second fan 34 through one branch, and then passes through the second fan 34 and is discharged to the path change valve group 39 through the second filter screen 32, in the path change valve group 39, the air passing through the second filter screen 32 is discharged into the exhaust pipe 38 through the branch of the one-way valve pointing to the exhaust pipe 38, so that the gas discharge process is completed, in the process, when the exhaust gas passes through the second filter screen 32, dust and impurities accumulated before passing through the second filter screen 32 can be taken away, and the purpose of cleaning the second filter screen 32 is achieved;

after the operation process shown in fig. 4 is performed for a period of time, the operation logic shown in fig. 5 is switched to, under the logic, the first fan 33 serves as an air outlet fan, the second fan 34 serves as an induced draft fan, at this time, in the route changing valve set 39, the branch is switched, namely, the branch of the air inlet 301 connected with the second fan 34 serves as an air inlet branch, the second filter screen 32 serves as a filter component, the branch from the first fan 33 to the exhaust pipe 38 serves as an air outlet branch, and the first filter screen 31 no longer serves as a filter component but is flushed and cleaned by air from the incubator 1;

As shown in fig. 1, a wire mesh plate 5 is arranged at an upper layer position of a water body in the sterilization filtrate tank 4, the wire mesh plate 5 is horizontally arranged, the aperture of the wire mesh is less than one millimeter, and the wire mesh plate 5 is positioned above the fresh air port 302.

After the gas emerges from the fresh air inlet 304, large-size bubbles may exist, the large-size bubbles are not fully contacted with the filtrate, the filtering and sterilizing effects are not significant, and after the gas is added into the wire mesh plate 5, the large-size bubbles reach the lower surface of the wire mesh plate 5, are cut into clusters of fine bubbles by the wire mesh plate 5, and are fully contacted with the sterilizing liquid, so that the operations of sterilizing and secondary filtering are completed.

As shown in fig. 1 and 3, the exhaust pipe 38 extends from the path switching valve set 39, bends to enter the sterilizing filtrate tank 4, and then extends to the outside atmosphere, and a differential pressure valve 382 is disposed on the exhaust pipe 38 and a section of the sterilizing filtrate tank 4 to the air outlet 304.

The presence of the pressure difference valve 382 has a flow resistance of the piping, so that the exhaust pipe 38 has a pressure one step higher than the exhaust port 304 (the external atmospheric pressure) on the section of the piping in front of the pressure difference valve 382, the higher pressure is approximately equal to the flow resistance of the pressure difference valve 382, the higher pressure needs to be pressurized by the first blower 33 or the second blower 34 to cause a pressure rise in the first half section of the exhaust pipe 38, after the pressure rise, the temperature of the gas rises, the gas exhausted from the exhaust pipe 38 is the air from the incubator 1, the incubator 1 is a "greenhouse" environment due to the requirement, the temperature is higher than the external atmosphere, if the incubator 1 is a large-scale incubator, if the gas heat in the part is directly exhausted out of the apparatus, a great amount of heat is wasted, and an additional heat supply is needed to heat the air from the outside into the incubator 1, so that the heat recovery process is preferably performed, the heat recovery of the invention is carried out by the exhaust pipe 38 and the differential pressure valve 382, the gas in the exhaust pipe 38 is compressed and heated, then the gas is bent and enters the sterilization filtrate tank 4, the temperature is transferred to the solution, and the solution heats the fresh air, thereby completing the heat recovery.

As shown in fig. 1 and 3, the exhaust pipe 38 is provided with a coil 381 at a section thereof located in the sterilizing filtrate tank 4, and the coil 381 is provided with fins on an outer surface thereof. The coil 381 and its surface fins enhance the heat exchange effect.

The differential pressure valve 382 is an automatic control valve, a position on the air inlet pipe 35 close to the air inlet 301 and a position on the air outlet pipe 38 close to the air outlet 304 are respectively connected to two ends of a temperature difference meter, the temperature difference meter detects the temperature difference between the air inlet 301 and the air outlet 304 and controls the differential pressure valve 382, and the larger the temperature difference between the air inlet 301 and the air outlet 304, the larger the flow resistance of the differential pressure valve 382 is.

One section of gas before the differential pressure valve 382 in the exhaust pipe 38 exchanges heat when flowing into the sterilization filter box 4, the temperature of the gas after heat exchange is slightly reduced, then the temperature can be spontaneously reduced when pressure reduction and gas expansion are carried out behind the differential pressure valve 382, if the temperature is reduced to the temperature of the outdoor atmosphere, then the balance can be established between the whole device and the external heat: the continuous intake and discharge of the gases into and out of the incubator 1, so that the total amount of the gases is constant, and then the temperatures at the inlet and outlet are uniform, so that the temperature in the incubator 1 can be maintained at a stable level, wherein the heat generation caused by the heat generation and flow resistance due to the work of the fan is not considered, and the heat is negligible compared to the large volume of the incubator 1 and the air flow with a large flow rate, or basically considered to be balanced with the heat dissipation of the outer surface of the apparatus, and if the temperature is stabilized very precisely, a heater is required to be added into the incubator 1.

The first fan 33 and the second fan 34 are bidirectional axial flow fans. The bidirectional axial flow fans can flow in two directions, and when one fan is used as a draught fan, the other fan is used as an air outlet fan.

A stirring blade is arranged in the sterilization filtrate tank 4. The solution in the sterilization filtrate tank 4 is stirred and turned by the stirring blade and is fully contacted with fresh air for sterilization. The bubbles of fresh air can be further refined.

The main use process of the device is as follows: outside air enters the device from the air inlet 301, is sucked by the first fan 33 or the second fan 34 as one of the induced draft fans, is injected into the sterilization filtrate box 4 through the fresh air inlet 302, when passing through the first filter screen 31 or the second filter screen 32, most of dust is filtered, the bubbles generated are cut into a cluster of small bubbles by the stirring blades and the wire mesh plate 5, the small bubbles are fully contacted with the sterilization liquid to perform sterilization and secondary filtration, the sterilization filtrate is filled into the incubator 1 to replace original dirty gas, the original gas enters the return air pipe 37 from the return air inlet 303, the dirty gas in the return air pipe 37 is discharged to the exhaust pipe 38 by the first fan 33 or the second fan 34 as one of the air outlet machines, when the discharged gas passes through the filter screens, the filter screens are cleaned, the filter screens can operate without maintenance for a long time, the gas in the exhaust pipe is compressed by the pressure difference valve 382, the temperature is raised, heat is transferred to the solution in the sterilization filtrate box 4, the temperature of the air is reduced by the expansion of the air itself behind the differential pressure valve 382, and the temperature of the air at the air outlet 304 is kept the same as that of the outside air entering the device, thereby stabilizing the temperature environment in the incubator 1.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. A cell culture apparatus for biotechnology research, characterized in that: the cell cultivation device comprises an incubator (1), frame plates (2), a ventilation module (3) and a sterilization filtrate box (4), wherein a plurality of layers of frame plates (2) are arranged at the upper part in the incubator (1) and used for placing cell cultivation dishes, the sterilization filtrate box (4) is arranged at the bottom of the incubator (1), the ventilation module (3) is arranged on the outer surface of the side surface of the incubator (1), fresh air is introduced into the ventilation module (3) from the outside and enters the sterilization filtrate box (4), the ventilation module (3) also sucks internal gas from the top in the incubator (1) and discharges the internal gas to the outside, and sterilization liquid is injected into the sterilization filtrate box (4);

the air exchange module (3) comprises a first filter screen (31), a second filter screen (32), a first fan (33), a second fan (34), an air inlet pipe (35), a fresh air pipe (36), an air return pipe (37), an air outlet pipe (38) and a path change valve group (39), wherein the path change valve group (39) is provided with four interfaces which are respectively connected with the air inlet pipe (35), the air outlet pipe (38), the first filter screen (31) and the second filter screen (32);

the other side of the first filter screen (31) is connected with a first fan (33), the other end of the second filter screen (32) is connected with a second fan (34), the other end of the first fan (33) is divided into two branches which are respectively connected with a fresh air pipe (36) and a return air pipe (37), the other end of the second fan (34) is also divided into two branches which are respectively connected with the fresh air pipe (36) and the return air pipe (37), one end, far away from the first fan (33), of the fresh air pipe (36) is a fresh air inlet (302), one end, far away from the first fan (33), of the return air pipe (37) is a return air inlet (303), the fresh air inlet (302) extends into the sterilization filtrate box (4), the return air inlet (303) is positioned at the upper part in the incubator (1), and two branches, connected with the first fan (33) and the second fan (34) respectively, on the fresh air pipe (36) are provided with one-way valves facing the fresh air inlet (302, two branches of the air return pipe (37) which are respectively connected with the first fan (33) and the second fan (34) are respectively provided with a one-way valve facing the air return opening (303);

one end, far away from the route changing valve group (39), of the air inlet pipe (35) is an air inlet (301), the air inlet (301) enters the route changing valve group (39) through a pipeline and then is connected with the first filter screen (31) and the second filter screen (32) through two branches, and one-way valves facing the first filter screen (31) and the second filter screen (32) are arranged in the route changing valve group (39) and on the two branches from the air inlet (301) to the first filter screen (31) and the second filter screen (32) respectively; exhaust pipe (38) keep away from one end of valve bank of changing a way (39) and be gas vent (304), gas vent (304) are connected first filter screen (31) and second filter screen (32) through two branch roads respectively after getting into valve bank of changing a way (39) via the pipeline, in valve bank of changing a way (39), gas vent (304) set up the check valve who deviates from first filter screen (31) and second filter screen (32) respectively on two branches of first filter screen (31) and second filter screen (32).

2. A cell culture apparatus for biotechnological research according to claim 1 characterised in that: the sterilization filter liquor box is characterized in that a wire mesh plate (5) is arranged at the upper layer of a water body in the sterilization filter liquor box (4), the wire mesh plate (5) is horizontally arranged, the aperture of a wire mesh is smaller than one millimeter, and the wire mesh plate (5) is positioned above the fresh air port (302).

3. A cell culture apparatus for biotechnological research according to claim 1 characterised in that: the exhaust pipe (38) extends from the path changing valve group (39), then bends to enter the sterilization filtrate tank (4), and then extends to be exhausted to the outside atmosphere, and a differential pressure valve (382) is arranged on the exhaust pipe (38) and on a section from the sterilization filtrate tank (4) to the air outlet (304).

4. A cell culture apparatus for biotechnological research according to claim 3 characterised in that: the section of the exhaust pipe (38) located in the sterilization filtrate box (4) is provided with a coil pipe (381), and fins are arranged on the outer surface of the coil pipe (381).

5. A cell culture apparatus for biotechnological research according to claim 3 characterised in that: the differential pressure valve (382) is an automatic control valve, the positions of the air inlet (301) and the exhaust pipe (38) on the air inlet pipe (35) and the exhaust port (304) are respectively connected to two ends of a temperature difference meter, the temperature difference meter detects the temperature difference between the air inlet (301) and the exhaust port (304) and controls the differential pressure valve (382), and the larger the temperature difference between the two is, the larger the flow resistance of the differential pressure valve (382) is.

6. A cell culture apparatus for biotechnological research according to claim 1 characterised in that: the first fan (33) and the second fan (34) are bidirectional axial fans.

7. A cell culture apparatus for biotechnological research according to claim 1 characterised in that: and a stirring blade is arranged in the sterilization filtrate tank (4).