CN110982686A - Microorganism observation test card, observation system and observation method - Google Patents
Microorganism observation test card, observation system and observation method Download PDFInfo
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- CN110982686A CN110982686A CN201911378941.8A CN201911378941A CN110982686A CN 110982686 A CN110982686 A CN 110982686A CN 201911378941 A CN201911378941 A CN 201911378941A CN 110982686 A CN110982686 A CN 110982686A
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- 244000005700 microbiome Species 0.000 title claims abstract description 66
- 238000012360 testing method Methods 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 11
- 239000012530 fluid Substances 0.000 claims abstract description 102
- 230000000813 microbial effect Effects 0.000 claims description 9
- 230000002906 microbiologic effect Effects 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 230000000877 morphologic effect Effects 0.000 claims description 3
- 230000008859 change Effects 0.000 abstract description 7
- 239000010408 film Substances 0.000 abstract description 6
- 239000010409 thin film Substances 0.000 abstract description 5
- 239000012528 membrane Substances 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 description 4
- 238000010191 image analysis Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract
The invention discloses a microorganism observation test card, which comprises a transparent main card body and a transparent film, wherein the end surface of the main card body is provided with a fluid introduction port, the upper surface of the main card body is provided with a plurality of fluid channels, a plurality of observation grooves and a plurality of sample pools, the fluid channels, the observation grooves and the sample pools are sequentially communicated, and the depth of the observation grooves is smaller than that of the sample pools; a membrane may cover the upper surface of the main card body and seal the fluid channel, the observation well and the sample cell. Because the depth of the observation groove is smaller than that of the sample cell, the observation groove can be fully squeezed by the fluid, and the depth of the fluid in the observation groove is small, so that the influence of the main card body, the thin film and the fluid with the overlarge depth on light rays can be reduced, and the light rays can easily pass through the observation groove and the fluid in the observation groove. The invention also discloses a microorganism observation system and an observation method, and the microorganism information acquisition device can be used for conveniently and rapidly observing the change of the microorganism sample.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a microorganism observation test card and an observation system.
Background
The culture and identification of microorganisms are of great significance for the research of microbial species, the research of clinical medicine and the like, and currently, a test card suitable for analyzing samples in an automatic instrument exists in the field of microbial detection. The automatic instrument has long time for culturing and detecting samples, and cannot quickly obtain results, but the change of microorganism samples in the fluid can be seen in short time through microscope observation, so that the results can be obtained, such as whether the microorganisms reproduce, the reproduction speed is high, and the forms are changed.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. Therefore, the invention provides a microorganism observation test card which is convenient for directly observing the change of a microorganism sample through a microscope.
The invention also provides a microorganism observation system with the microorganism observation test card and a microorganism observation method.
The microorganism observation test card comprises a transparent main card body and a transparent film, wherein a fluid introduction port is formed in the end face of the main card body, a plurality of fluid channels, a plurality of observation grooves and a plurality of sample pools are formed in the upper surface of the main card body, the fluid channels, the observation grooves and the sample pools are communicated in sequence, and the depth of each observation groove is smaller than that of each sample pool; the thin film may cover an upper surface of the main card body and seal the fluid channel, the observation groove, and the sample cell.
The microorganism observation test card provided by the embodiment of the invention has at least the following beneficial effects: the fluid reaches the sample cell after passing through fluid introduction port, fluid passage, observation groove in proper order, because the degree of depth of observation groove is than the degree of depth of sample cell is little, can be crowded to fill just by the fluid in the observation groove the degree of depth of fluid in the observation groove is little, is favorable to reducing the influence of the too big fluid of degree of depth self to light, and light can pass easily the observation groove with the fluid in the observation groove, can directly observe with the microscope the fluid of observation groove department, can observe out to have in the fluid whether microorganism sample or microorganism sample take place slight change.
According to some embodiments of the invention, a positioning protrusion is arranged in the observation groove, and the upper surface of the positioning protrusion is lower than the upper surface of the main card body.
According to some embodiments of the invention, the positioning protrusion has a smooth surface.
According to some embodiments of the invention, the fluid channel comprises at least one main channel and a plurality of distribution channels, one end of the main channel is communicated with the fluid introduction port, one end of the distribution channel is communicated with the main channel, and the other end is communicated with the observation tank.
According to some embodiments of the invention, a side of the sample cell remote from the observation groove is communicated with a bubble collecting groove.
According to some embodiments of the invention, the sample wells are distributed in a matrix on the upper surface of the main card body.
According to some embodiments of the invention, the number of sample wells is 60-140.
According to some embodiments of the invention, the sample cell is oblong in cross-section.
The microorganism observation system according to the second aspect of the present invention includes a microorganism information collection device including a microscope, and the microorganism observation test card described above. The test card is used for loading a sample, and the change of the microorganism sample in the fluid can be conveniently and quickly known through the matching of the microorganism information acquisition device and the test card.
According to a method for observing microorganisms of an embodiment of the third aspect of the present invention, the above-mentioned microorganism observation system is used, and the method comprises the steps of preparing a fluid containing or suspected of containing a microorganism sample; filling fluid into the test card: covering a film on the upper surface of the test card, connecting a conduit on the fluid introduction port, placing the test card, the conduit and the fluid in a negative pressure device, filling the fluid into the conduit, the fluid channel, the observation groove and the sample cell in sequence by the negative pressure device, and filling the observation groove and the sample cell with the fluid; collecting microorganism image information: and (3) placing the test card carrying the fluid in an incubation device, incubating the fluid, placing the test card in a microorganism information acquisition device, and acquiring morphological information of the microorganism, so as to analyze whether the microorganism exists and the propagation and growth conditions of the microorganism. The observation system and the observation method are matched for use, so that the information such as the existence of microorganisms in the fluid, the propagation and growth conditions of the microorganisms and the like can be quickly and conveniently known.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a schematic view of a microbiological observation test card according to an embodiment of the present invention;
FIG. 2 is a schematic cross-sectional view of the microbiological observation test card shown in FIG. 1.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number.
In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.
Referring to fig. 1 to 2, a microorganism observation test card includes a transparent main card body 100 and a transparent film, a fluid inlet 200 is provided on an end surface of the main card body 100, a plurality of fluid channels 300, a plurality of observation grooves 400 and a plurality of sample wells 500 are provided on an upper surface of the main card body 100, the fluid channels 300, the observation grooves 400 and the sample wells 500 are sequentially communicated, a depth of the observation grooves 400 is smaller than a depth of the sample wells 500, and a bottom of the observation grooves 400 is lower than an upper surface of the main card body 100; a thin film may cover the upper surface of the main card body 100 and seal the fluid channel 300, the observation tank 400, and the sample cell 500.
When the test card is used, the thin film covers the upper surface of the main card body 100, and seals the fluid channel 300, the observation groove 400 and the sample cell 500, so that the fluid introduction port 200, the fluid channel 300, the observation groove 400 and the sample cell 500 form a communication network, fluid enters the test card through the fluid introduction port 200, and then the fluid channel 300, the observation groove 400 and the sample cell 500 are sequentially filled with the fluid, because the depth of the observation groove 400 is smaller than that of the sample cell 500, the observation groove 400 can be filled with the fluid, and the depth of the fluid in the observation groove 400 is smaller, which is beneficial to reducing the influence of the fluid with too large depth on the light, the light can easily pass through the fluid in the observation groove 400 and the observation groove 400, the fluid in the observation groove 400 is observed by a microscope, and whether a microorganism sample exists in the fluid or not or the microorganism sample generates slight change, and can be observed. .
Referring to fig. 2, a positioning protrusion 410 is disposed in the observation groove 400, and an upper surface of the positioning protrusion 410 is lower than an upper surface of the main card body 100. When observing with a microscope, the positioning protrusion 410 can be just corresponding to the lower part of the objective lens to improve the speed of adjusting the position of the observed sample at the stage, and the smaller the height difference between the upper surface of the positioning protrusion 410 and the upper surface of the main card body 100, the smaller the depth of the fluid at the observation groove 400, and the more beneficial the light can pass through the fluid. In some embodiments, the width of the observation groove 400 is equal to the width of the sample cell 500, the outlines of the observation groove 400 and the sample cell 500 form an oval on the upper surface of the main card body 100, and the width of the positioning protrusion 410 is smaller than the width of the observation groove 400.
In some embodiments of the present invention, the surface of the positioning protrusion 410 is flat and smooth. The positioning protrusion 410 is in a column shape, and the diameter of the positioning protrusion 410 is smaller than the width of the observation groove 400, and further, the diameter of the positioning protrusion 410 is preferably half of the width of the observation groove 400. When the observation groove 400 is opened, the positioning protrusion 410 may be opened at the same time. Of course, the positioning protrusion 410 may have other shapes, such as a square body.
In some embodiments of the present invention, the fluid channel 300 includes at least one main channel 310 and a plurality of distribution channels 320, one end of the main channel 310 communicates with the fluid introduction port 200, one end of the distribution channels 320 communicates with the main channel 310, and the other end communicates with the observation tank 400. The fluid introduced into the fluid introduction port 200 is divided into a plurality of sub-streams after entering the main channel 310, and each sub-stream enters one of the distribution channels 320 and then enters the observation tank 400 and the sample cell 500. The width of the main channel 310 is greater than the width of the dispensing channel 320, so that the main channel 310 has a greater fluid transfer capacity than the dispensing channel 320, thereby satisfying the requirement of a plurality of dispensing channels 320 and increasing the fluid filling speed of the test card. In some embodiments, the sample wells 500 are divided into two groups, one group is provided with two main channels 310, and the two main channels 310 correspond to the two groups of sample wells 500, so as to further increase the speed of conveying fluid into the sample wells 500.
In some embodiments of the present invention, a side of the sample cell 500 away from the observation groove 400 is connected to a bubble collecting groove 600. When fluid is filled, the fluid inlet 200 is communicated with the conduit to draw air out of the test card, then the free end of the conduit is immersed into the fluid test tube, and finally the fluid in the test tube is filled into the test card under the action of external air pressure, in the process, residual gas in the fluid channel 300, the observation groove 400 and the sample cell 500 is collected in the bubble collecting groove 600, and bubbles in the observation groove 400 are avoided. In some embodiments, the bubble collection tank 600 is egg-shaped.
In some embodiments of the present invention, the sample wells 500 are distributed on the upper surface of the main card body 100 in a matrix, and the ordered arrangement of the sample wells 500 is beneficial to marking or distinguishing each sample well 500, and is beautiful. The number of the sample pools 500 is 60-140, a large amount of sample data can be obtained by cultivating or observing samples once, a plurality of test cards are not needed, and the workload of personnel is reduced. Referring to fig. 1, in the present embodiment, the number of sample cells 500 is eight rows by twelve columns.
In some embodiments of the present invention, the main card body 100 is a square body with a shape specification and is convenient to take. A transition surface is arranged between two adjacent end surfaces of the main card body 100, so that the upper surface and the lower surface of the main card body 100 can be identified conveniently. The main card body 100 and the film are made of transparent materials and have good light transmittance.
In some embodiments of the present invention, the cross section of the sample cell 500 is oblong, and the length direction of the oblong is consistent with the row direction of each row of sample cells 500, so that the arrangement of the sample cells 500 is more orderly and compact, and the space of the test card can be fully utilized.
The invention also provides a microorganism observation system, which comprises a microorganism information acquisition device and the microorganism observation test card in any embodiment, wherein the microorganism information acquisition device comprises a microscope. The fluid sample is filled in the test card, the test card is placed on a microscope stage, and a microscope is adjusted, so that people can observe the change of the sample through the microscope. The microorganism information acquisition device also comprises an image analysis system, and the image analysis system analyzes the acquired fluid image information so as to know whether microorganisms exist in the fluid, whether the microorganisms reproduce, the speed of the reproduction, whether the form is changed and the like.
The invention also provides a microorganism observation method, which adopts the microorganism observation system and comprises the following steps of preparing a fluid containing or suspected to contain a microorganism sample; filling fluid into the test card: covering a film on the upper surface of the test card, connecting a conduit to the fluid inlet 200, placing the test card, the conduit and the fluid in a negative pressure device, filling the fluid into the conduit, the fluid channel 300, the observation tank 400 and the sample cell 500 in sequence by the negative pressure device, and filling the observation tank 400 and the sample cell 500 with the fluid; collecting microorganism image information: and (3) placing the test card carrying the fluid in an incubation device, incubating the fluid, placing the test card in a microorganism information acquisition device, and acquiring microorganism morphological information so as to analyze whether microorganisms exist and the growth condition of the microorganisms. The observation system and the observation method are matched for use, so that the information such as the existence of microorganisms in the fluid, the propagation and growth conditions of the microorganisms and the like can be quickly and conveniently known.
In some embodiments, filling the fluid into the test card comprises the steps of covering the top surface of the test card with a thin film, attaching a conduit to the fluid inlet 200, placing the test card, conduit and fluid into a negative pressure chamber of a negative pressure device, with the free end of the conduit extending into a beaker or tube containing the fluid, evacuating the negative pressure chamber, and drawing air from the fluid inlet 200, fluid channel 300, observation well 400 and sample cell 500, thereby creating a negative pressure within the test card, and then returning the negative pressure chamber to atmospheric pressure, whereby the fluid can be expressed into the test card by the atmosphere.
After the microorganism information acquisition device acquires the image information of the fluid, the image information can be directly presented to people for direct observation, and the growth and reproduction conditions and the forms of the microorganisms can also be analyzed and judged by using an image analysis system.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention.
Claims (10)
1. A microbiological observation test card comprising:
the end face of the main card body is provided with a fluid inlet, the upper surface of the main card body is provided with a plurality of fluid channels, a plurality of observation grooves and a plurality of sample pools, the fluid channels, the observation grooves and the sample pools are sequentially communicated, and the depth of the observation grooves is smaller than that of the sample pools;
and the transparent film can cover the upper surface of the main card body and seal the fluid channel, the observation groove and the sample pool.
2. The microbial observation test card of claim 1, wherein: a positioning bulge is arranged in the observation groove, and the upper surface of the positioning bulge is lower than the upper surface of the main card body.
3. The microbial observation test card of claim 2, wherein: the surface of the positioning bulge is flat and smooth.
4. The microbial observation test card of any one of claims 1 to 3, wherein: the fluid passage includes at least one main passage and a plurality of distribution passages, one end of the main passage communicates with the fluid introduction port, one end of the distribution passage communicates with the main passage, and the other end communicates with the observation tank.
5. The microbial observation test card of any one of claims 1 to 3, wherein: and one side of the sample pool, which is far away from the observation groove, is communicated with a bubble collecting groove.
6. The microbial observation test card of claim 1, wherein: the sample pools are distributed on the upper surface of the main card body in a matrix manner.
7. The microbial observation test card of claim 1 or 6, wherein: the number of the sample pools is 60-140.
8. The microbial observation test card of claim 1, wherein: the cross section of the sample cell is oblong.
9. A microbial observation system, comprising: comprising a microbiological information collection device including a microscope and a microbiological observation test card according to any one of claims 1 to 8.
10. A method for observing a microorganism, comprising the step of using the system for observing a microorganism according to claim 9,
preparing a fluid containing or suspected of containing a sample of a microorganism;
filling fluid into the test card: covering a film on the upper surface of the test card, connecting a conduit on the fluid introduction port, placing the test card, the conduit and the fluid in a negative pressure device, filling the fluid into the conduit, the fluid channel, the observation groove and the sample cell in sequence by the negative pressure device, and filling the observation groove and the sample cell with the fluid;
collecting microorganism image information: and (3) placing the test card carrying the fluid in an incubation device, incubating the fluid, placing the test card in a microorganism information acquisition device, and acquiring microorganism morphological information so as to analyze whether microorganisms exist and the propagation and growth conditions of the microorganisms.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911378941.8A CN110982686A (en) | 2019-12-27 | 2019-12-27 | Microorganism observation test card, observation system and observation method |
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| CN201911378941.8A CN110982686A (en) | 2019-12-27 | 2019-12-27 | Microorganism observation test card, observation system and observation method |
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2019
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Address after: 519041 No. 19 Golden Coast Road, San Zao Town, Jin Wan District, Zhuhai, Guangdong Applicant after: Zhuhai deer Bioengineering Co.,Ltd. Address before: 519041 No. 19 Golden Coast Road, San Zao Town, Jin Wan District, Zhuhai, Guangdong Applicant before: ZHUHAI DL MEDICAL BIOTECH Co.,Ltd. |
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| CB02 | Change of applicant information |