CN110960268B

CN110960268B - Tissue minimally invasive sampler for livestock

Info

Publication number: CN110960268B
Application number: CN202010008598.4A
Authority: CN
Inventors: 李孝文; 樊士冉; 刘聪; 张志勇; 樊铭玉
Original assignee: Xiajin New Hope Liuhe Agriculture And Animal Husbandry Co ltd; Beijing New Hope Liuhe Biotechnology Industry Group Co ltd; Shandong New Hope Liuhe Group Co Ltd; New Hope Liuhe Co Ltd
Current assignee: Xiajin New Hope Liuhe Agriculture And Animal Husbandry Co ltd; Beijing New Hope Liuhe Biotechnology Industry Group Co ltd; Shandong New Hope Liuhe Group Co Ltd; New Hope Liuhe Co Ltd
Priority date: 2020-01-06
Filing date: 2020-01-06
Publication date: 2023-04-21
Anticipated expiration: 2040-01-06
Also published as: CN110960268A

Abstract

The invention discloses a tissue minimally invasive sampler for livestock, and belongs to the technical field of special equipment for animal husbandry. The technical proposal is as follows: the tissue minimally invasive sampler for the livestock comprises a needle cylinder, push rods penetrating through two ends of the needle cylinder, sealing heads arranged at the tail end of the needle cylinder and movably connected to the corresponding ends of the push rods, and trocars sleeved at the head end of the needle cylinder and in sliding fit with the push rods along the central line direction of the push rods. The beneficial effects of the invention are as follows: the livestock tissue minimally invasive sampler provided by the invention has the advantages of high sampling efficiency, short sampling time, less bleeding of living tissues, simple structure and operation, ingenious design, good adaptability, time and labor saving and high application value.

Description

Tissue minimally invasive sampler for livestock

Technical Field

The invention relates to the technical field of special equipment for animal husbandry, in particular to a tissue minimally invasive sampler for livestock.

Background

In the African swine fever situation, the pig industry is faced with a great challenge. Early diagnosis and accurate elimination in the non-pestilence disease-causing pig group are important for controlling and purifying the African swine fever, and epidemic losses can be effectively reduced. At present, pigs only sample and collect nose and anus swabs, oral liquid and the like for early non-pestilence diagnosis, but false positives or false negatives often occur, the early diagnosis of epidemic situation is affected, and the rejection time is delayed. The lymphoid organ is the preferred site of virus implantation, and can detect the non-pestilence pathogen earlier and more reliably.

However, the collection of the lymph node tissues of the living pigs is difficult in the past, and the application and popularization of the detection and diagnosis modes of the lymph node pathogens of the living pigs are affected. The development of a rapid and effective tissue sampling tool is a subject to be solved urgently.

Disclosure of Invention

In order to solve the problems of difficult collection of living pig lymph node tissues and the like, the invention provides the livestock tissue minimally invasive sampler which is efficient in sampling, short in sampling time, less in bleeding of living tissues, simple in structure and operation, ingenious in design, good in adaptability, time-saving and labor-saving, and high in application value.

In order to achieve the aim, the invention provides a tissue minimally invasive sampler for livestock, which comprises a needle cylinder, push rods penetrating through two ends of the needle cylinder, sealing heads movably connected to corresponding ends of the push rods, sleeve needles sleeved on corresponding ends of the push rods and in sliding fit with the push rods along the central line direction of the push rods, wherein the sealing heads are arranged at the tail ends of the needle cylinder;

further, the trocar comprises a needle stem, a needle bolt arranged at one end part of the needle stem, a needle point arranged at the other end of the needle stem, a sampling hole arranged on the side wall of the needle stem and close to the needle point, and a barb arranged at the sampling hole and close to the end part of the needle point.

When sampling, the end part of the push rod, which is close to the head end of the needle cylinder, is arranged at one side of the sampling hole, which is far away from the needle point, the sampler pierces the trocar from the needle point into the tissue to be sampled, and then the trocar is pulled out, and at the moment, sampling substances are left in the sampling hole due to the existence of the barb; during inspection, the end part of the push rod, which is close to the tail end of the needle cylinder, is pressed until the end part of the push rod, which is close to the head end of the needle cylinder, reaches the front end position of the needle passing point, and in the pushing process of the push rod, sampling substances reserved in the sampling hole are pushed out of the designated container by the needle point, so that the method has the effects of simplicity in operation, high efficiency in sampling, short sampling time and less bleeding of living tissues.

Further, the cross section of push rod with trocar sliding fit tip, with needle stalk inner wall's cross section phase-match guarantees that the sampling substance that remains in the sampling hole can once only be released completely, improves sampling efficiency.

Further, the livestock tissue minimally invasive sampler further comprises a rebound structure arranged between the needle cylinder and the push rod, so that the push rod can be conveniently restored after the sampling substance is pushed out, namely, the end part of the push rod, which is close to the head end of the needle cylinder, returns to one side of the sampling hole, which is far away from the needle point, and the sampling efficiency is further improved.

Furthermore, the end of the push rod, which is close to the tail end of the needle cylinder, is provided with a clamping ring, and the rebound structure comprises a spring arranged between the clamping ring and the needle cylinder, so that the structure is simple and the operation is convenient.

Further, the snap ring is located in the needle cylinder, and comprises a first snap ring close to the tail end of the needle cylinder, the first snap ring and the end socket form a limiting relationship, when the push rod is prevented from being rebounded, the push rod is separated from the trocar to be matched with the needle, and/or the push rod is ejected out of the needle cylinder, and a second snap ring close to the head end of the needle cylinder is used for conveniently providing a supporting surface for the expansion and contraction of the spring, and the spring is sleeved on the push rod and is located between the second snap ring and the head end of the needle cylinder.

Further, the push rod comprises a base rod and a guide rod which are sequentially connected along the direction of the central line, and the clamping ring arranged on the guide rod, so that two ends of the push rod can be rapidly distinguished, and the sampler can be rapidly installed.

Further, the cross section size of the base rod is larger than that of the guide rod, so that the rigidity of the push rod is ensured, and bending deformation is prevented during pressing.

Furthermore, the clamping ring is connected with the push rod in a clamping way, so that the sealing head is convenient to install.

Further, the livestock tissue minimally invasive sampler further comprises a plug arranged between the needle cylinder and the trocar, and the trocar is prevented from being separated from the head end of the needle cylinder when the needle is pulled out.

Further, the plug is in threaded connection with the head end of the needle cylinder.

Furthermore, the head end of the needle cylinder is provided with a nipple, the center line of the nipple is coincident with the center line of the needle cylinder, on one hand, the needle sleeve is convenient to connect, and on the other hand, the guide rod can be guided in the installation process, so that the guide rod is in quick fit with the needle sleeve.

The beneficial effects of the invention are as follows: (1) The animal tissue minimally invasive sampler provided by the invention is simple to operate and efficient in sampling, and solves the problem of difficult traditional sampling;

(2) By using the animal tissue minimally invasive sampler provided by the invention, the sampling time is short, the bleeding of living tissues is less, and the pollution of pathogens to the environment is effectively reduced;

(3) The animal tissue minimally invasive sampler provided by the invention has the advantages of simple structure, high reliability, good adaptability, strong maintainability and the like, and has a simple use process and extremely high application value.

(4) The livestock tissue minimally invasive sampler provided by the invention has ingenious design, and fully combines the use scenes during the design, so that the matching performance is higher, the problem of difficult collection of the living pig lymph node tissue in the past is solved to a great extent, and the labor and time cost are saved, so that the livestock tissue minimally invasive sampler has obvious advantages.

Drawings

Fig. 1 is a schematic view of the external appearance structure of embodiment 1 of the present invention;

FIG. 2 is a schematic view of the structure of embodiment 1 of the present invention taken along the center line of the cylinder;

FIG. 3 is a schematic view of a push rod according to embodiment 2 of the present invention;

FIG. 4 is a schematic structural view of a snap ring according to embodiment 2 of the present invention;

FIG. 5 is a schematic view of another snap ring according to embodiment 2 of the present invention;

FIG. 6 is a schematic diagram showing the relationship between the seal head and the push rod in embodiment 3 of the present invention;

FIG. 7 is a schematic diagram showing the relationship between the seal head and the push rod in embodiment 4 of the present invention;

FIG. 8 is a schematic view showing the appearance of the structure of the present invention in examples 5-6;

FIG. 9 is a schematic structural view of a limiting plate in embodiment 6 of the present invention;

fig. 10 is a schematic structural diagram of a plug in embodiment 6 of the present invention;

fig. 11 is a schematic view showing the appearance structure of

embodiments

5 and 7 of the present invention;

FIG. 12 is a schematic view of a limiting plate according to embodiment 7 of the present invention;

fig. 13 is a schematic structural diagram of a plug in embodiment 7 of the present invention;

fig. 14 is a left side view of the stop collar of embodiments 5-7 of the present invention.

Wherein, the reference numerals are as follows: 1. a push rod; 1-1, a base rod; 1-1-1, a connecting groove; 1-1-2, a pressing part; 1-2, a guide rod; 1-3, snap ring; 1-3-a, a first snap ring; 1-3-b, a second snap ring; 1-3-1, a ring body; 1-3-2, a clamping part; 1-3-3, a missing part; 2. a seal head; 2-1, oblong through holes; 3. a spring; 4. a needle cylinder; 4-1, nipple; 5. a trocar; 5-1, a pintle; 5-2, needle stems; 5-3, a needle point; 5-4, sampling holes; 5-5, barbs; 6. a plug; 6-1, a chute; 6-2, a limit groove; 7. a limit sleeve; 7-1, a slot; 7-2, an arc limiting end; 8. a limiting piece; 8-a, a first limiting piece; 8-a-1, a first card; 8-a-2, a first limiting block; 8-a-3, a first sync block; 8-a-4, a threaded column; 8-b, a second limiting piece; 8-b-1, a second card; 8-b-2, a second limiting block; 8-b-3, a second sync block; 9. a fastener.

Detailed Description

In order to clearly illustrate the technical characteristics of the scheme, the scheme is explained below through a specific embodiment.

Example 1

Referring to fig. 1 to 2, the embodiment of the invention provides a tissue minimally invasive sampler for livestock, which comprises a needle cylinder 4 with a nipple 4-1 at the head, a push rod 1 penetrating through two ends of the needle cylinder 4 and composed of a base rod 1-1, a guide rod 1-2 and a clamping ring 1-3, a seal head 2 sleeved on the base rod 1-1 and slidingly matched with the base rod 1-1, a seal head 2 arranged at the tail end of the needle cylinder 4, a sleeve needle 5 arranged at the end of the nipple 4-1, a sleeve needle 5 sleeved on the base rod 1-1, a spring 3 arranged between the head end of the needle cylinder 4 and the clamping ring 1-3, and a plug 6 arranged between the needle cylinder 4 and the sleeve needle 5;

the trocar 5 comprises a needle stem 5-2, a needle bolt 5-1 arranged at one end of the needle stem 5-2, a needle tip 5-3 arranged at the other end of the needle stem 5-2, a sampling hole 5-4 arranged on the side wall of the needle stem 5-2 and close to the needle tip 5-3, and a barb 5-5 arranged at the end of the sampling hole 5-4 and close to the needle tip 5-3.

Preferably, the clamping ring 1-3 is positioned in the needle cylinder 4 and comprises a first clamping ring 1-3-a close to the tail end of the needle cylinder 4 and a second clamping ring 1-3-b close to the head end of the needle cylinder 4, and the spring 3 is sleeved on the push rod 1 and positioned between the second clamping ring 1-3-b and the head end of the needle cylinder 4;

preferably, the tail end of the needle cylinder 4 is in threaded connection or interference fit with the seal head 2;

preferably, the cross section of the guide rod 1-2 is matched with the cross section of the inner wall of the needle stalk 5-2, or the cross section of the end of the guide rod 1-2 away from the base rod 1-1 is matched with the cross section of the inner wall of the needle stalk 5-2;

preferably, the needle stem 5-2 penetrates through the plug 6, the needle bolt 5-1 is clamped with one end of the plug 6, and the other end of the plug 6 is in threaded connection or clamping connection with the head end of the needle cylinder 4;

example 2

Referring to fig. 3 to 5, on the basis of embodiment 1, the other is the same as embodiment 1, except that embodiment 1: the base rod 1-1 is connected with the clamping ring 1-3 in a clamping way;

two connecting grooves 1-1-1 are formed in the outer circumferential surface of the base rod 1-1 along the circumferential direction, wherein one connecting groove 1-1 is close to a pressing part 1-1-2 of the base rod 1-1, and the other connecting groove 1-1 is close to a guide rod 1-2;

preferably, the snap ring 1-3 comprises a ring body 1-3-1 and an elastic clamping part 1-3-2 arranged on the inner wall of the ring body 1-3-1; the inner diameter of the ring body 1-3-1 is slightly larger than the outer diameter of the base rod 1-1, and the clamping part 1-3-2 is matched with the connecting groove 1-1-1; in a further preferred embodiment, the ring body 1-3-1 is made of a rigid material;

in another preferred scheme, the clamping ring 1-3 comprises an elastic ring body 1-3-1, the elastic ring body 1-3-1 is arranged on the ring body 1-3-1 along the radial direction, and the missing parts 1-3-3 penetrating through the inner wall and the outer wall of the ring body 1-3-1; the inner diameter of the ring body 1-3-1 is slightly smaller than the outer diameter of the base rod 1-1, and the inner wall of the ring body 1-3-1 is matched with the connecting groove 1-1-1; the clamping ring 1-3 can be installed at the connecting groove 1-1-1 or detached by applying outward force on two sides of the missing part 1-3-3.

Example 3

Referring to fig. 6, on the basis of example 1, the other is the same as example 1, except that example 1: the base rod 1-1 is fixedly connected with the snap ring 1-3, and the pressing part 1-1-2 positioned at the end part of the base rod 1-1 is detachably connected with the base rod 1-1.

Example 4

Referring to fig. 7, on the basis of example 1, the other is the same as example 1, except that example 1: the base rod 1-1 is fixedly connected with the clamping ring 1-3, the shape of the clamping ring 1-3 is set to be oblong, and the sealing head 2 is provided with an oblong through hole 2-1 matched with the clamping ring 1-3.

When the push rod 1 or the seal head 2 is disassembled and assembled, the clamping ring 1-3 is matched with the long round through hole 2-1; when in use, the push rod 1 or the seal head 2 is rotated, so that the clamping ring 1-3 and the seal head 2 can realize a limiting relationship.

Example 5

Referring to fig. 8 to 14, on the basis of embodiment 1, the other is the same as embodiment 1, except that embodiment 1: a chute 6-1 is formed in the circumferential surface of the outer side of the plug 6 along the direction parallel to the central line of the plug 6, a plurality of limit grooves 6-2 communicated with the chute 6-1 are formed in the circumferential direction, and at least one end of the chute 6-1 extends to the end face of the plug 6;

the livestock tissue minimally invasive sampler further comprises a limiting component arranged on the plug 6 and/or the needle cylinder 4, and the limiting component is preferably arranged on the plug 6; the exposed length of the needle tip 5-3 can be controlled, thereby precisely controlling the penetration depth at the time of sampling.

The limiting assembly comprises a limiting sleeve 7 and a limiting piece 8 arranged on the limiting sleeve 7;

the limit piece 8 is matched with the chute 6-1 and/or the limit groove 6-2;

preferably, the limit sleeve 7 is in a cylindrical shape with an inner diameter matched with the maximum outer diameter of the plug 6; one end of the cylindrical limiting sleeve 7 is provided with an inward-bent arc limiting end 7-2, and the end surface of the other end is provided with a slot 7-1 along the direction parallel to the central line of the slot; the limiting piece 8 is inserted into the slot 7-1, and the limiting piece 8 and the slot 7-1 are in sliding fit with each other;

the limiting piece 8 is provided with a limiting block, the limiting block is in sliding fit with the sliding groove 6-1 to limit and adjust the limiting assembly, and the limiting block is clamped with the limiting groove 6-2 to position the limiting assembly.

Example 6

Referring to fig. 8, 9, 10, 14, on the basis of example 5, the other is the same as example 5, except that example 5: the limiting assembly comprises a limiting sleeve 7, a first limiting piece 8-a arranged on the limiting sleeve 7, and a fastening piece 9 arranged between the limiting sleeve 7 and the first limiting piece 8-a; the length of the first limit piece 8-a is smaller than that of the slot 7-1;

the first limiting piece 8-a comprises a first plugboard 8-a-1, a first limiting piece 8-a-2 arranged on one side surface of the first plugboard 8-a-1, and a threaded column 8-a-4 arranged on the other side surface of the first plugboard 8-a-1 and used for connecting a fastener 9;

preferably, the first plugboard 8-a-1 is made of transparent materials, so that the first plugboard 8-a-1 is convenient to install, and the first limiting block 8-a-2 is matched with the chute 6-1 quickly, the limiting groove 6-2 is found quickly, the first limiting block 8-a-2 is buckled with the chute, and the adjustment efficiency is further improved;

preferably, the first limiting piece 8-a further comprises a first synchronizing block 8-a-3 corresponding to the first limiting piece 8-a-2 arranged on the side surface of the first plugboard 8-a-1 provided with the threaded column 8-a-4, so that the position of the first limiting piece 8-a-2 can be determined more intuitively;

preferably, the number of the limit grooves 6-2 is one.

When the sampling tool is used, the limiting sleeve 7 is sleeved on the plug 6 and rotated until the slot 7-1 is close to the chute 6-1, the first plugboard 8-a-1 is inserted into the slot 7-1, the first limiting block 8-a-2 slides along the chute 6-1 to the limiting groove 6-2, the first limiting block 8-a-2 is buckled with the limiting groove 6-2, the position of the limiting sleeve 7 is adjusted, the distance from the needle point 5-3 to the arc limiting end 7-2 is proper, and the fastening piece is fastened, so that the sampling operation can be performed.

Example 7

Referring to fig. 11 to 14, on the basis of embodiment 5, the other is the same as embodiment 5, except that embodiment 5: the limiting piece 8 is arranged as a second limiting piece 8-b, the length dimension of the second limiting piece 8-b is the same as that of the slot 7-1, and at least one group of opposite side surfaces are clung to the corresponding inner wall of the slot 7-1;

the second limiting piece 8-b comprises a second plugboard 8-b-1 and a plurality of second limiting blocks 8-b-2 which are uniformly arranged on one side surface of the second plugboard 8-b-1;

preferably, the second insert plate 8-b-1 is made of transparent material, and the function and effect of the first insert plate 8-a-1 are similar to those of the transparent material in embodiment 6;

preferably, the second limiting piece 8-b is arranged on the other side surface of the first plugboard 8-b-1, and the second synchronizing blocks 8-b-3 corresponding to the second limiting pieces 8-b-2 respectively have similar actions and effects as those of the first synchronizing blocks 8-a-3 corresponding to the first limiting pieces 8-a-2 in the embodiment 6;

preferably, limit grooves 6-2 are formed in two sides of the sliding groove 6-1, so that limit precision of the limit sleeve 7 is improved;

preferably, limit grooves 6-2 on two sides of the sliding groove 6-1 are mutually staggered;

preferably, the distances between two adjacent limit grooves 6-2 on the same side are equal;

preferably, the distance between two adjacent limit grooves 6-2 on one side is equal to the distance between two adjacent limit grooves 6-2 on the other side;

preferably, the distance between two adjacent second limiting blocks 8-b-2 is equal to the distance between the first limiting groove 6-2 and the second limiting groove 6-2 on the same side, and the adjustment continuity is improved.

When the sampling device is used, the second plugboard 8-b-1 is inserted into the slot 7-1, the slot 7-1 is closely aligned with the chute 6-1, then the limiting sleeve 7 is sleeved on the plug 6, the second limiting block 8-b-2 slides along the chute 6-1, the position of the limiting sleeve 7 is adjusted, the distance from the needle point 5-3 to the arc limiting end 7-2 is proper, the limiting sleeve 7 is rotated relatively, and the second limiting block 8-b-2 is buckled with the limiting groove 6-2 at the corresponding position, so that sampling operation can be performed.

The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.

Claims

1. The tissue minimally invasive sampler for the livestock is characterized by comprising a needle cylinder (4), push rods (1) penetrating through two ends of the needle cylinder (4), a seal head (2) arranged at the tail end of the needle cylinder (4), a sleeve needle (5) arranged at the head end of the needle cylinder (4) and sleeved at the end part of the push rod (1) and in sliding fit with the push rod (1) along the central line direction of the push rod (1);

the trocar (5) comprises a needle stem (5-2), a pintle (5-1) arranged at one end of the needle stem (5-2), a needle point (5-3) arranged at the other end of the needle stem (5-2), a sampling hole (5-4) arranged on the side wall of the needle stem (5-2) and close to the needle point (5-3), and a barb (5-5) arranged at the end of the sampling hole (5-4) and close to the needle point (5-3);

the cross section of the sliding fit end part of the push rod (1) and the trocar (5) is matched with the cross section of the inner wall of the needle stalk (5-2);

the livestock tissue minimally invasive sampler also comprises a rebound structure arranged between the needle cylinder (4) and the push rod (1);

the push rod (1) comprises a base rod (1-1) and a guide rod (1-2) which are sequentially connected along the central line direction of the push rod (1), and a clamping ring (1-3) arranged on the guide rod (1-2); the clamping ring (1-3) is positioned in the needle cylinder (4) and comprises a first clamping ring (1-3-a) close to the tail end of the needle cylinder (4) and a second clamping ring (1-3-b) close to the head end of the needle cylinder (4);

the rebound structure comprises a spring (3) sleeved on the push rod (1) and arranged between the second clamping ring (1-3-b) and the head end of the needle cylinder (4);

the cross section of the guide rod (1-2) is matched with the cross section of the inner wall of the needle stem (5-2), or the cross section of the end part of the guide rod (1-2) away from the base rod (1-1) is matched with the cross section of the inner wall of the needle stem (5-2);

the livestock tissue minimally invasive sampler further comprises a plug (6) arranged between the needle cylinder (4) and the trocar (5);

the base rod (1-1) is fixedly connected with the clamping ring (1-3), the clamping ring (1-3) is in an oblong shape, and the sealing head (2) is provided with an oblong through hole (2-1) matched with the clamping ring (1-3);

a chute (6-1) is formed in the outer circumferential surface of the plug (6) along the direction parallel to the central line of the plug (6), a plurality of limit grooves (6-2) communicated with the chute (6-1) are formed in the circumferential direction, and at least one end of the chute (6-1) extends to the end face of the plug (6); the animal tissue minimally invasive sampler also comprises a limiting component arranged on the plug (6);

the limiting assembly comprises a limiting sleeve (7), a first limiting piece (8-a) arranged on the limiting sleeve (7), and a fastening piece (9) arranged between the limiting sleeve (7) and the first limiting piece (8-a);

the limiting sleeve (7) is arranged in a cylindrical shape with the inner diameter matched with the maximum outer diameter of the plug (6); one end of the cylindrical limit sleeve (7) is provided with an inward-bent arc limit end (7-2), and the end surface of the other end is provided with a slot (7-1) along the direction parallel to the cylindrical central line;

the length of the first limiting piece (8-a) is smaller than that of the slot (7-1); the first limiting piece (8-a) comprises a first inserting plate (8-a-1), a first limiting block (8-a-2) arranged on one side surface of the first inserting plate (8-a-1), and a threaded column (8-a-4) arranged on the other side surface of the first inserting plate (8-a-1) and used for connecting the fastening piece (9);

the first plugboard (8-a-1) is made of transparent materials;

the first limiting piece (8-a) further comprises a first synchronous block (8-a-3) which is arranged on the side surface of the first plugboard (8-a-1) provided with the threaded column (8-a-4) and corresponds to the first limiting piece (8-a-2);

when the sampling device is used, the limiting sleeve (7) is sleeved on the plug (6) and rotated until the slot (7-1) is close to the sliding groove (6-1) to align, the first inserting plate (8-a-1) is inserted into the slot (7-1), the first limiting block (8-a-2) slides along the sliding groove (6-1) to the limiting groove (6-2), the first limiting block (8-a-2) is buckled with the limiting groove (6-2), the position of the limiting sleeve (7) is adjusted until the distance from the needle tip (5-3) to the arc limiting end (7-2) is proper, and the fastening piece is fastened to sample the sampling device.

2. The animal tissue minimally invasive sampler according to claim 1, characterized in that the snap ring (1-3) is in clamping connection with the push rod (1).

3. The animal tissue minimally invasive sampler according to any of claims 1-2, characterized in that the head end of the needle cylinder (4) is provided with a nipple (4-1).